Pathogens最新文献

{"title":"Clinical Outcomes and Virulence Factors of Shiga Toxin-Producing <i>Escherichia coli</i> (STEC) from Southern Alberta, Canada, from 2020 to 2022.","authors":"Heather Glassman, Vivien Suttorp, Theron White, Kim Ziebell, Ashley Kearney, Kyrylo Bessonov, Vincent Li, Linda Chui","doi":"10.3390/pathogens13100822","DOIUrl":"https://doi.org/10.3390/pathogens13100822","url":null,"abstract":"<p><p>Shiga toxin-producing <i>Escherichia coli</i> (STEC) can cause severe clinical disease in humans, particularly in young children. Recent advances have led to greater availability of sequencing technologies. We sought to use whole genome sequencing data to identify the presence or absence of known virulence factors in all clinical isolates submitted to our laboratory from Southern Alberta dated 2020-2022 and correlate these virulence factors with clinical outcomes obtained through chart review. Overall, the majority of HUS and hospitalizations were seen in patients with O157:H7 serotypes, and HUS cases were primarily in young children. The frequency of virulence factors differed between O157:H7 and non-O157 serotypes. Within the O157:H7 cases, certain virulence factors, including <i>espP</i>, <i>espX1</i>, and <i>katP</i>, were more frequent in HUS cases. The number of samples was too low to determine statistical significance.</p>","PeriodicalId":19758,"journal":{"name":"Pathogens","volume":null,"pages":null},"PeriodicalIF":3.3,"publicationDate":"2024-09-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11510307/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142505387","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Virus-Specific Nanobody-Chimeras Degrade the Human Cytomegalovirus US28 Protein in CD34+ Cells.","authors":"Emma Poole, Janika Schmitt, Stephen C Graham, Bernard T Kelly, John Sinclair","doi":"10.3390/pathogens13100821","DOIUrl":"https://doi.org/10.3390/pathogens13100821","url":null,"abstract":"<p><p>After primary infection, human cytomegalovirus (HCMV) establishes lifelong persistence, underpinned by latent carriage of the virus with spontaneous reactivation events. In the immune-competent, primary infection or reactivation from latency rarely causes disease. However, HCMV can cause significant disease in immune-compromised individuals such as immune-suppressed transplant patients. Latency, where the viral genome is carried in the absence of the production of infectious virions, can be established in undifferentiated cells of the myeloid lineage. A number of stimuli can cause virus reactivation from latency to occur, beginning with the induction of viral immediate-early (IE) lytic gene expression. The suppression of viral IE gene expression to establish and maintain latent infection is known to result from a balance of viral and cellular factors. One key viral factor involved in this is the G protein-coupled receptor US28. Recently, we have shown that US28 is targeted for degradation by a modified nanobody (PCTD-Vun100bv) based on the novel PACTAC (PCSK9-antibody clearance-targeting chimeras) approach for targeted protein degradation. Furthermore, we have shown that this PCTD-Vun100bv-induced degradation of US28 results in IE gene expression in experimentally latently infected CD14+ monocytes. However, HCMV also establishes latency in CD34+ bone marrow cells, the progenitors of CD14+ cells. Here, we show that PCTD-Vun100bv also causes US28 degradation in these CD34+ primary cells, again resulting in the induction of viral IE gene expression. Additionally, we show that PCTD-Vun100bv can target US28 in naturally latently infected CD14+ monocytes from an HCMV-seropositive donor, allowing these latently infected cells to be killed by HCMV-specific cytotoxic T cells from that same donor. These observations support the view that targeting US28 for degradation during natural latency could be a tractable 'shock-and-kill' strategy to target the latent HCMV reservoir in myeloid cells.</p>","PeriodicalId":19758,"journal":{"name":"Pathogens","volume":null,"pages":null},"PeriodicalIF":3.3,"publicationDate":"2024-09-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11510245/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142505444","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Toxicity of Common Acaricides, Disinfectants, and Natural Compounds against Eggs of <i>Rhipicephalus annulatus</i>.","authors":"Samar M Ibrahium, Abdel-Azeem S Abdel-Baki, Sahar M Gadelhaq, Shawky M Aboelhadid, Hesham A Mahran, Saleh Al-Quraishy, Abdulrahman Reyad, Asmaa A Kamel","doi":"10.3390/pathogens13100824","DOIUrl":"https://doi.org/10.3390/pathogens13100824","url":null,"abstract":"<p><p>Ticks pose a significant threat due to their ability to lay thousands of eggs, which can persist in the environment for extended periods. While the impact of various compounds on adult and larval ticks has been studied, research on their efficacy against tick eggs is limited. This study evaluated the ovicidal activity of commercial acaricides, disinfectants, and natural products against <i>Rhipicephalus annulatus</i> eggs using the egg hatch assay (EHA). Deltamethrin and cypermethrin caused a non-significant inhibition of hatching (IH%), even at concentrations higher than the recommended levels. By contrast, the acaricides chlorpyrifos, phoxim, and amitraz significantly inhibited hatching at all tested concentrations. Ivermectin also demonstrated significant IH% at various concentrations but did not fully inhibit the hatching process. Among the disinfectants tested, Virkon-S^®, TH4, and Chlorox showed insignificant effects, whereas formalin achieved an IH% of only 34.1% at a high concentration of 200 mg/mL. Natural products, carvacrol and thymol, exhibited significant ovicidal activity, with a significant IH%. In a semi-field application, phoxim (0.5 mg/mL) and deltamethrin (0.05 mg/mL) were sprayed on tick eggs on pasture soil from a farm. The results indicated that phoxim-treated eggs had a 40% IH%, while deltamethrin-treated eggs showed only an 8.79% IH%. In conclusion, the acaricides amitraz, phoxim, and chlorpyrifos, as well as the natural products carvacrol and thymol, caused significant toxicity to <i>R. annulatus</i> eggs.</p>","PeriodicalId":19758,"journal":{"name":"Pathogens","volume":null,"pages":null},"PeriodicalIF":3.3,"publicationDate":"2024-09-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11510607/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142505439","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Advances in the Search for SARS-CoV-2 M^pro and PL^pro Inhibitors.","authors":"Marcel Arruda Diogo, Augusto Gomes Teixeira Cabral, Renata Barbosa de Oliveira","doi":"10.3390/pathogens13100825","DOIUrl":"https://doi.org/10.3390/pathogens13100825","url":null,"abstract":"<p><p>SARS-CoV-2 is a spherical, positive-sense, single-stranded RNA virus with a large genome, responsible for encoding both structural proteins, vital for the viral particle's architecture, and non-structural proteins, critical for the virus's replication cycle. Among the non-structural proteins, two cysteine proteases emerge as promising molecular targets for the design of new antiviral compounds. The main protease (M^pro) is a homodimeric enzyme that plays a pivotal role in the formation of the viral replication-transcription complex, associated with the papain-like protease (PL^pro), a cysteine protease that modulates host immune signaling by reversing post-translational modifications of ubiquitin and interferon-stimulated gene 15 (ISG15) in host cells. Due to the importance of these molecular targets for the design and development of novel anti-SARS-CoV-2 drugs, the purpose of this review is to address aspects related to the structure, mechanism of action and strategies for the design of inhibitors capable of targeting the M^pro and PL^pro. Examples of covalent and non-covalent inhibitors that are currently being evaluated in preclinical and clinical studies or already approved for therapy will be also discussed to show the advances in medicinal chemistry in the search for new molecules to treat COVID-19.</p>","PeriodicalId":19758,"journal":{"name":"Pathogens","volume":null,"pages":null},"PeriodicalIF":3.3,"publicationDate":"2024-09-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11510351/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142505324","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Is Short-Read 16S rRNA Sequencing of Oral Microbiome Sampling a Suitable Diagnostic Tool for Head and Neck Cancer?","authors":"Kenny Yeo, Fangmeinuo Wu, Runhao Li, Eric Smith, Peter-John Wormald, Rowan Valentine, Alkis James Psaltis, Sarah Vreugde, Kevin Fenix","doi":"10.3390/pathogens13100826","DOIUrl":"https://doi.org/10.3390/pathogens13100826","url":null,"abstract":"<p><p>The oral microbiome, studied by sampling the saliva or by oral rinse, has been long thought to have diagnostic capacity for head and neck cancers (HNC). However, previous reports on the HNC oral microbiome provide inconsistent results. The aim of this study is to consolidate these datasets and determine the oral microbial composition between HNC patients to healthy and premalignant individuals. We analyzed 16 published head and neck cancer (HNC) short-read 16S rRNA sequencing datasets, specifically targeting the V3V4, V4 and V4V5 regions. These datasets included saliva and oral rinse samples from donors with HNC, as well as from healthy and premalignant donors. Differences in diversities and microbial abundance were determined. HNC saliva displayed lower alpha diversity than healthy donors. In contrast, the opposite trend was observed for oral rinse samples. Beta diversity scores were largely similar across different patient types. Similar oral phyla were detected for all samples, but proportions were largely dependent on sample type (i.e., saliva or oral rinse) and primer set utilized for 16S rRNA sequencing. <i>Neisseria</i>, <i>Leptotrichia</i> and <i>Megasphaera</i> were elevated in healthy saliva, while <i>Mycoplasma</i> was elevated in HNC saliva. Oral rinse and saliva displayed similar enrichment for <i>Fusobacterium</i>, while <i>Veillonella</i>, <i>Alloprevotella</i>, and <i>Campylobacter</i> showed conflicting results. The sparse partial least squares discriminant analysis model performed effectively in discriminating HNC from healthy or premalignant patients using V3V4 saliva (AUC = 0.888) and V3V4 oral rinse (AUC = 0.928), while poor discriminative capacity was observed for V4 saliva (AUC = 0.688). In conclusion, our meta-analysis highlighted the limitations of 16S rRNA sequencing, particularly due to variations across study batches, primer sets (i.e., V3V4, V4), and sample types. Hence, caution should be exercised when interpreting 16S rRNA sequencing results across studies, especially when different primer sets and sample types are used.</p>","PeriodicalId":19758,"journal":{"name":"Pathogens","volume":null,"pages":null},"PeriodicalIF":3.3,"publicationDate":"2024-09-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11510575/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142505424","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The Ubiquitous Wilt-Inducing Pathogen <i>Fusarium oxysporum</i>-A Review of Genes Studied with Mutant Analysis.","authors":"Edan Jackson, Josh Li, Thilini Weerasinghe, Xin Li","doi":"10.3390/pathogens13100823","DOIUrl":"https://doi.org/10.3390/pathogens13100823","url":null,"abstract":"<p><p><i>Fusarium oxysporum</i> is one of the most economically important plant fungal pathogens, causing devastating Fusarium wilt diseases on a diverse range of hosts, including many key crop plants. Consequently, <i>F. oxysporum</i> has been the subject of extensive research to help develop and improve crop protection strategies. The sequencing of the <i>F. oxysporum</i> genome 14 years ago has greatly accelerated the discovery and characterization of key genes contributing to <i>F. oxysporum</i> biology and virulence. In this review, we summarize important findings on the molecular mechanisms of <i>F. oxysporum</i> growth, reproduction, and virulence. In particular, we focus on genes studied through mutant analysis, covering genes involved in diverse processes such as metabolism, stress tolerance, sporulation, and pathogenicity, as well as the signaling pathways that regulate them. In doing so, we hope to present a comprehensive review of the molecular understanding of <i>F. oxysporum</i> that will aid the future study of this and related species.</p>","PeriodicalId":19758,"journal":{"name":"Pathogens","volume":null,"pages":null},"PeriodicalIF":3.3,"publicationDate":"2024-09-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11510031/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142505457","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Performance of Fujifilm Dengue NS1 Antigen Rapid Diagnosis Kit Compared to Quantitative Real-Time Polymerase Chain Reaction.","authors":"Mya Myat Ngwe Tun, Merveille Kapandji, Atsuhiko Wada, Ko Yamamoto, Shyam Prakash Dumre, Khine Mya Nwe, Htin Lin, Yuki Takamatsu, Kyaw Zin Thant, Hlaing Myat Thu, Takeshi Urano, Basu Dev Pandey, Kouichi Morita","doi":"10.3390/pathogens13090818","DOIUrl":"https://doi.org/10.3390/pathogens13090818","url":null,"abstract":"<p><p>Dengue is a viral infection caused by the dengue virus (DENV), transmitted to humans through the bite of infected <i>Aedes</i> mosquitoes. About half of the world's population is now at risk of dengue, which represents a global public health concern, especially in tropical and subtropical countries. Early detection of the viral infection is crucial to manage the disease; hence, effective rapid diagnostic tests are essential. In this study, we evaluated the performance between the new Fujifilm Dengue non-structural antigen diagnosis kit (FF NS1 kit) and the SD Bioline NS1 antigen test kit (SD NS1 kit) against the quantitative real-time polymerase chain reaction (qRT-PCR) assays. The 140 acute serum samples collected from the Yangon General Hospital and Yangon Children's Hospital, Myanmar, from 2017 to 2019 were characterised by the three assays. With the qRT-PCR as the standard, the FF NS1 kit and the SD NS1 kit exhibited sensitivity of 94.3% and 88.6%, respectively, and specificity of 100% in both kits. Moreover, the positivity rates of the FF NS1 kit and the SD NS1 kit were 97.5% and 95% in primary infection and 90% and 80% in secondary infection, respectively. Our overall results suggest that the FF NS1 kit is reliable and accurate for detecting DENV infection.</p>","PeriodicalId":19758,"journal":{"name":"Pathogens","volume":null,"pages":null},"PeriodicalIF":3.3,"publicationDate":"2024-09-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11434953/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142351480","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Prospective Analysis of Safety and Efficacy of Tenofovir Alafenamide Fumarate (TAF) in European Real-World Patients with Chronic Hepatitis B: A Single-Centre Real-Word Cohort Study.","authors":"Balazs Fülöp, Janett Fischer, Magdalena Hahn, Albrecht Böhlig, Madlen Matz-Soja, Thomas Berg, Florian van Bömmel","doi":"10.3390/pathogens13090820","DOIUrl":"https://doi.org/10.3390/pathogens13090820","url":null,"abstract":"<p><p><b>Background:</b> Tenofovir alafenamide (TAF) is a novel prodrug of tenofovir for the treatment of chronic hepatitis B (CHB) that has shown a favourable renal safety profile while offering suppression of HBV DNA similar to tenofovir disoproxil fumarate (TDF). We aimed to study changes in markers of HBV replication and renal function in a real-world setting in European patients. <b>Methods:</b> In our prospective single-arm, non-interventional observational study, HBeAg-positive and HBeAg-negative patients with chronic HBV mono-infection receiving TAF as their first or following line treatment were enrolled. HBV DNA, HBsAg, markers of bone metabolism, and renal function were determined at baseline and every consecutive 3 months. <b>Results:</b> A total of 50 patients (70% male) were included. The mean duration of TAF treatment was 18 (3-36) months. In 20 patients with detectable HBV DNA at baseline, median serum levels of HBV DNA log₁₀ changed from 2.33 (0.766-6.47) to 1.04 IU/mL at the end of observation and became undetectable in 11 patients. Median HBsAg log₁₀ decreased from 3.37 (0.88-5.10) to 2.39 (1.52-4.19) IU/mL. During the entire observation period, the renal function parameters remained stable in patients with normal renal function and even in those with renal dysfunction. Mild adverse events were reported by 14 patients (28%). <b>Conclusions:</b> TAF was a safe and effective treatment, also in patients with decreased renal function.</p>","PeriodicalId":19758,"journal":{"name":"Pathogens","volume":null,"pages":null},"PeriodicalIF":3.3,"publicationDate":"2024-09-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11434708/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142351482","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Oral Microbiota and the Risk of Gastrointestinal Cancers-A Narrative Literature Review.","authors":"Kinga Knop-Chodyła, Anna Kochanowska-Mazurek, Zuzanna Piasecka, Aneta Głaz, Ewelina Weronika Wesołek-Bielaska, Kinga Syty, Alicja Forma, Jacek Baj","doi":"10.3390/pathogens13090819","DOIUrl":"https://doi.org/10.3390/pathogens13090819","url":null,"abstract":"<p><p>The human body is colonized by trillions of microorganisms in a symbiotic relationship. The oral cavity represents one of the most abundant microbial habitats in our body. Advances in sequencing techniques provide a more detailed understanding of the oral microbiota and how imbalances between bacteria, the phenomenon of dysbiosis, can affect not only the development of dental caries or inflammation within the oral cavity but also systemic diseases and cancers in distant locations. This narrative review evaluates the relationship between oral microbiota and its impact on gastrointestinal cancers. Using the keywords \"oral microbiota 'AND' gastrointestinal cancers\", the PubMed Web of Science and Scopus databases were searched for articles published between 2014 and 2024. Based on the review, the relationship between oral microbiota and oral, esophageal, gastric, colorectal, hepatocellular, and pancreatic cancers was described. Potential oncogenic mechanisms exploited by the microbiota such as the production of pro-inflammatory cytokines, induction of abnormal immune responses, and disruption of cell metabolic pathways were assessed. Further research and a thorough understanding of the impact of the oral microbiota on the development of cancers of the gastrointestinal tract may play a key role in their prevention, diagnosis, and treatment in the future.</p>","PeriodicalId":19758,"journal":{"name":"Pathogens","volume":null,"pages":null},"PeriodicalIF":3.3,"publicationDate":"2024-09-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11434710/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142351478","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Patient Experiences and Perceptions with Infections Due to Multidrug-Resistant Organisms: A Systematic Review.","authors":"Mousa M AlRawashdeh, Angela Ishak, Ahmed Al-Bunnia, Aris P Agouridis, Theodore Lytras, Nikolaos Spernovasilis, Constantinos Tsioutis","doi":"10.3390/pathogens13090817","DOIUrl":"https://doi.org/10.3390/pathogens13090817","url":null,"abstract":"<p><p>Infections by multidrug-resistant organisms (MDROs) pose significant public health challenges, including increased mortality rates, healthcare costs, and significant impacts on the quality of life for patients. Utilizing a systematic review methodology adhering to PRISMA guidelines, we performed a comprehensive search across three databases, identifying 20 relevant studies that investigated the psychological effects of infections due to MDROs on hospitalized adults. The primary outcomes examined included depression, anxiety, and other psychosocial impacts, while secondary outcomes included patient and caregiver understanding of the infection. Findings revealed consistent associations between contact isolation due to MDRO infections and heightened levels of depression and anxiety among patients, although evidence regarding the impact on anger was mixed. Other psychological aspects, such as feelings of stigmatization and reduced healthcare provider interactions, were also recorded. The current systematic review highlights the importance of addressing these psychological effects through holistic, patient-centered care approaches, emphasizing the need for better communication and comprehensive education for both patients and healthcare providers. Our findings suggest that mitigating the psychological burden of MDROs can enhance overall patient care and outcomes and call for further research to optimize care strategies for patients hospitalized for infections due to MDROs.</p>","PeriodicalId":19758,"journal":{"name":"Pathogens","volume":null,"pages":null},"PeriodicalIF":3.3,"publicationDate":"2024-09-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11435282/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142351479","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}