Pathogens最新文献_第10页

From Culture-Negative to DNA-Positive: The Molecular Revolution in Infective Endocarditis Diagnosis. 从培养阴性到dna阳性：感染性心内膜炎诊断的分子革命。

IF 3.3 3区医学

Pathogens Pub Date : 2025-05-23 DOI: 10.3390/pathogens14060518

Myeongji Kim, Madiha Fida, Omar M Abu Saleh, Nischal Ranganath

{"title":"From Culture-Negative to DNA-Positive: The Molecular Revolution in Infective Endocarditis Diagnosis.","authors":"Myeongji Kim, Madiha Fida, Omar M Abu Saleh, Nischal Ranganath","doi":"10.3390/pathogens14060518","DOIUrl":"10.3390/pathogens14060518","url":null,"abstract":"Infective endocarditis (IE) remains a diagnostic challenge, particularly in cases where microbiological diagnosis is not established. Advances in molecular diagnostics have expanded the ability to identify causative pathogens beyond traditional culture-based methods. This review explores the role of molecular assays, including pathogen-specific PCR, multiplex PCR, broad-range PCR, and shotgun metagenomic sequencing, in diagnosing IE. These molecular techniques enhance pathogen detection, especially in patients with prior antibiotic exposure, and improve diagnostic accuracy in culture-negative IE. Broad-range PCR assays and metagenomic sequencing offer the untargeted detection of a wide spectrum of organisms. Despite their advantages, limitations such as availability, interpretation challenges, and a lack of antimicrobial susceptibility testing remain. A multimodal approach integrating molecular diagnostics with conventional methods is essential to optimize patient management. Further research is needed to refine diagnostic algorithms and improve cost-effectiveness in clinical practice.","PeriodicalId":19758,"journal":{"name":"Pathogens","volume":"14 6","pages":""},"PeriodicalIF":3.3,"publicationDate":"2025-05-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12196431/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144485439","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Legionella spp. in a Dental Office-Current State of Knowledge. 军团菌在牙科诊所的现状。

IF 3.3 3区医学

Pathogens Pub Date : 2025-05-22 DOI: 10.3390/pathogens14060512

Jolanta Szymańska

引用次数: 0

Novel Insight into Culicoides (Diptera: Ceratopogonidae) Host Preferences and the First Evidence of Avian Haemosporidian Parasites in Biting Midges in Slovakia. 对库蠓（双翅目：蠓科）寄主偏好的新认识和斯洛伐克蠓中禽嗜血虫寄生虫的首个证据。

IF 3.3 3区医学

Pathogens Pub Date : 2025-05-22 DOI: 10.3390/pathogens14060515

Nikola Janošková, Andrea Schreiberová, Ľuboš Korytár, Lenka Minichová, Alica Kočišová

{"title":"Novel Insight into Culicoides (Diptera: Ceratopogonidae) Host Preferences and the First Evidence of Avian Haemosporidian Parasites in Biting Midges in Slovakia.","authors":"Nikola Janošková, Andrea Schreiberová, Ľuboš Korytár, Lenka Minichová, Alica Kočišová","doi":"10.3390/pathogens14060515","DOIUrl":"10.3390/pathogens14060515","url":null,"abstract":"Culicoides biting midges (Diptera: Ceratopogonidae) are important vectors of avian haemosporidian parasites. Understanding their host preferences is crucial for elucidating transmission routes of vector-borne pathogens. In Slovakia, such knowledge is limited, particularly in forested wetlands. This study aimed to identify Culicoides species, their host preferences, and haemosporidian parasites in a wetland ecosystem at the Bird Ringing Station in Drienovec. Midges were collected in 2022 using UV light traps at two sites. In total, 2344 Culicoides individuals of 19 species were collected. Host blood was identified and DNA subsequently extracted from 36 engorged females, revealing feeding on three mammal and five bird species. The most frequently identified host was roe deer (Capreolus capreolus), predominantly fed upon by Culicoides obsoletus (Meigen 1818). Notably, avian haemosporidian DNA was detected for the first time in Slovakia in three Culicoides females. In two Culicoides alazanicus Dzhafarov 1961 individuals, DNA of Haemoproteus asymmetricus (TUPHI01) and Plasmodium matutinum (LINN1) was confirmed, both associated with avian blood from Turdus sp. One Culicoides festivipennis Kieffer 1914 female carried Haemoproteus tartakovskyi (HAWF1) and fed on Coccothraustes coccothraustes. These findings highlight the potential role of local Culicoides species in transmitting avian pathogens and underscore the importance of monitoring their ecology.","PeriodicalId":19758,"journal":{"name":"Pathogens","volume":"14 6","pages":""},"PeriodicalIF":3.3,"publicationDate":"2025-05-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12196307/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144485456","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Mycobacterium fortuitum Infection Mimicking an Odontogenic Abscess: Report of a Pediatric Case with Relevant Literature Analysis. 模拟牙源性脓肿的运气分枝杆菌感染：1例儿科病例报告并相关文献分析。

IF 3.3 3区医学

Pathogens Pub Date : 2025-05-22 DOI: 10.3390/pathogens14060513

Giuseppe Barile, Luisa Limongelli, Marta Forte, Tommaso Corsalini, Saverio Capodiferro, Massimo Corsalini

{"title":"Mycobacterium fortuitum Infection Mimicking an Odontogenic Abscess: Report of a Pediatric Case with Relevant Literature Analysis.","authors":"Giuseppe Barile, Luisa Limongelli, Marta Forte, Tommaso Corsalini, Saverio Capodiferro, Massimo Corsalini","doi":"10.3390/pathogens14060513","DOIUrl":"10.3390/pathogens14060513","url":null,"abstract":"Non-tuberculous mycobacteria (NTM) are saprophytes of both soil and water that may cause infection with a high risk of dissemination, mainly in immunocompromised patients. Most NTM infections occur in the lungs, while uncommon localizations are the skin, soft tissues, musculoskeletal apparatus, and lymphatic system. The possible relationship between NTM infections and dental procedures is still unclear. The authors reported a rare manifestation of NTM infection occurring in a 6-year-old girl who developed sub-mandibular swelling related to a necrotic tooth, thus mimicking an abscess of odontogenic origin. Fine-needle aspiration biopsy of the sub-mandibular swelling and the following microbiologic investigation showed infection sustained by the Mycobacterium fortuitum complex. After the medical and surgical treatment, the patient completely recovered after 8 months. A review of the relevant literature was carried out to deepen the clinical and microbiological aspects of such a rare occurrence.","PeriodicalId":19758,"journal":{"name":"Pathogens","volume":"14 6","pages":""},"PeriodicalIF":3.3,"publicationDate":"2025-05-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12195853/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144485375","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Discovery and Genomic Characterisation of Novel Papillomaviruses in Australian Wild Birds. 澳大利亚野生鸟类新型乳头瘤病毒的发现和基因组特征。

IF 3.3 3区医学

Pathogens Pub Date : 2025-05-22 DOI: 10.3390/pathogens14060514

Subir Sarker, Vasilli Kasimov, Md Mizanur Rahaman, Babu Kanti Nath, Martina Jelocnik

{"title":"Discovery and Genomic Characterisation of Novel Papillomaviruses in Australian Wild Birds.","authors":"Subir Sarker, Vasilli Kasimov, Md Mizanur Rahaman, Babu Kanti Nath, Martina Jelocnik","doi":"10.3390/pathogens14060514","DOIUrl":"10.3390/pathogens14060514","url":null,"abstract":"Papillomaviruses are small, circular DNA viruses that infect epithelial and mucosal cells, which have co-evolved with their hosts over time. While certain mammalian papillomaviruses-especially those linked to disease-are well studied, there is limited knowledge about papillomaviruses associated with avian species. In this study, we identified two avian papillomaviruses from eye/choana swabs of the sacred kingfisher (Todiramphus sanctus) and the little corella (Cacatua sanguinea), collected in Queensland, Australia. The genomes of these viruses, designated as todiramphus sanctus papillomavirus 1 (TsPV1) and cacatua sanguinea papillomavirus 1 (CsPV1), were found to be 7883 and 7825 base pairs in length, respectively. The TsPV1 and CsPV1 genomes exhibited the highest nucleotide sequence identity (>56%) with papillomavirus genomes previously sequenced from mallards or wild ducks in the United States, followed by those from black-legged kittiwakes and Atlantic puffins (>54%) in Newfoundland, Canada. Both TsPV1 and CsPV1 share approximately a 65% nucleotide sequence identity in the L1 gene with anas platyrhynchos papillomavirus 3 (AplaPV3), indicating that they represent novel avian papillomaviruses. Notably, the two genomes in this study were nearly identical (99.69%), and their L1 proteins shared 100% sequence identity. Phylogenetic analysis positioned TsPV1 and CsPV1 within a clade of avian papillomaviruses associated with closely related avian hosts, including the mallard, African grey parrot, common chaffinch, and Atlantic canary. These findings underscore the importance of further research on studying additional Australian bird species longitudinally, which will help to establish potential disease associations and ecological impacts of previously unrecognised and novel papillomaviruses in Australian wild birds.","PeriodicalId":19758,"journal":{"name":"Pathogens","volume":"14 6","pages":""},"PeriodicalIF":3.3,"publicationDate":"2025-05-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12196351/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144485426","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Development of the Roe Deer-Fascioloides magna Association over Time. 鹿-大片形虫协会随时间的发展。

IF 3.3 3区医学

Pathogens Pub Date : 2025-05-22 DOI: 10.3390/pathogens14060516

Anja France Noëlle Renée Buet, Miljenko Bujanić, Krešimir Krapinec, Ivica Bošković, Anđelko Gašpar, Dean Konjević

{"title":"Development of the Roe Deer-Fascioloides magna Association over Time.","authors":"Anja France Noëlle Renée Buet, Miljenko Bujanić, Krešimir Krapinec, Ivica Bošković, Anđelko Gašpar, Dean Konjević","doi":"10.3390/pathogens14060516","DOIUrl":"10.3390/pathogens14060516","url":null,"abstract":"The trematode Fascioloides magna is originally a parasite of North American deer species. Upon its arrival to Europe, F. magna met new intermediate and final hosts. Depending on the type of host, the clinical picture, pathological findings, epidemiology and outcome can vary significantly. As an aberrant host, it was long believed that the roe deer (Capreolus capreolus) fails to develop pseudocysts, and therefore the infected animal dies before the parasite can mature and start to produce eggs. In this study, 676 roe deer livers were collected in Croatia during the hunting years of four consecutive years (2019-2023) in Bjelovar-Bilogora County (BB), and 184 livers were collected from Zagreb County (ZG) in the hunting year 2022/2023. Livers were analysed macroscopically and on a cut surface for lesions and any developmental stage of F. magna according to a standard protocol. The mean prevalence of infected livers during the whole study period was 12.86% in BB and 3.8% in ZG. No pseudocysts were detected in samples from ZG, while there was an increasing trend of pseudocyst presence over time in BB. The occurrence of pseudocysts in infected livers showed a rapid increase after the hunting season 2019/2020, before becoming constant (at approx. 40%). The odds of finding pseudocysts ranged between 2.7 (OR = 2.7317, CI 95% 0.3108 to 24.0095, p = 0.365) and 2.9 (OR = 2.9167, CI 95% 0.3163 to 26.8924, p = 0.345) times higher in later years compared to 2019/2020. Similarly, an increasing trend (though less pronounced) was observed in the numbers of livers simultaneously containing pseudocysts and fluke migratory stages. The results indicate a potential change in the roe deer-F. magna association, where an increasing number of roe deer are forming pseudocysts and can survive even multiple infections.","PeriodicalId":19758,"journal":{"name":"Pathogens","volume":"14 6","pages":""},"PeriodicalIF":3.3,"publicationDate":"2025-05-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12195886/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144485424","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Broad Antifungal Spectrum of the Pore-Forming Peptide C14R Against Cryptococcus and Candida Species from the WHO Fungal Priority Pathogens List. 成孔肽C14R对WHO真菌重点病原体清单中隐球菌和念珠菌的广谱抗真菌作用

IF 3.3 3区医学

Pathogens Pub Date : 2025-05-22 DOI: 10.3390/pathogens14060511

Carolina Firacative, Norida Vélez, Ann-Kathrin Kissmann, Daniel Alpízar-Pedraza, Jan-Christoph Walter, Ludger Ständker, Frank Rosenau

{"title":"Broad Antifungal Spectrum of the Pore-Forming Peptide C14R Against Cryptococcus and Candida Species from the WHO Fungal Priority Pathogens List.","authors":"Carolina Firacative, Norida Vélez, Ann-Kathrin Kissmann, Daniel Alpízar-Pedraza, Jan-Christoph Walter, Ludger Ständker, Frank Rosenau","doi":"10.3390/pathogens14060511","DOIUrl":"10.3390/pathogens14060511","url":null,"abstract":"The World Health Organization (WHO) prioritized 19 fungal species based on the significant impact of these pathogens on human health, including the emergence of antifungal resistance, which highlights the necessity of finding new antifungal therapies. Among these novel therapeutic approaches, the antimicrobial pore-forming peptide C14R has shown to be promising against Candida albicans and Candida auris. In this study, the antifungal in vitro efficacy of C14R was assessed against six additional species from the WHO priority list, Cryptococcus neoformans, Cryptococcus gattii, Candida glabrata, Candida tropicalis, Candida parapsilosis and Candida krusei, as well as against Candida dubliniensis. This study shows that C14R has good antifungal activity against several clinical isolates of the studied species, with MIC values between 0.8476 and 10.88 µg/mL. Most notably, some of the studied isolates are resistant to commonly used antifungal drugs but are susceptible to the peptide. C14R showed, moreover, its capacity to disrupt Cryptococcus capsules, beyond its already proven capacity to disrupt plasma membranes, and its antifungal activity was not affected depending on the serotype or species assessed. The inclusion of basidiomycete and ascomycete yeasts allowed us to display the broad-spectrum potential of C14R, highlighting it as a promising candidate as an antifungal agent.","PeriodicalId":19758,"journal":{"name":"Pathogens","volume":"14 6","pages":""},"PeriodicalIF":3.3,"publicationDate":"2025-05-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12196350/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144485407","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Evaluation of the Standard M10 MTB/NTM Molecular Test for the Rapid Identification of Tuberculous and Nontuberculous Mycobacteria in Liquid Cultures. 液体培养物中结核和非结核分枝杆菌快速鉴定标准M10 MTB/NTM分子检测方法的评价

IF 3.3 3区医学

Pathogens Pub Date : 2025-05-22 DOI: 10.3390/pathogens14060517

Sara Caldrer, Alberta Carrara, Andrea Ragusa, Lavinia Nicolini, Elena Pomari, Cristina Mazzi, Fabio Formenti, Francesca Perandin

{"title":"Evaluation of the Standard M10 MTB/NTM Molecular Test for the Rapid Identification of Tuberculous and Nontuberculous Mycobacteria in Liquid Cultures.","authors":"Sara Caldrer, Alberta Carrara, Andrea Ragusa, Lavinia Nicolini, Elena Pomari, Cristina Mazzi, Fabio Formenti, Francesca Perandin","doi":"10.3390/pathogens14060517","DOIUrl":"10.3390/pathogens14060517","url":null,"abstract":"Since 2013, the World Health Organization has recommended the use of rapid molecular tests as the initial diagnostic step for Mycobacterium tuberculosis (MTB) infection to enhance the control of tuberculosis. In recent years, the prevalence of infections by non-tuberculous mycobacteria (NTM) in humans has also risen, particularly in countries with low tuberculosis incidence, such as Italy. Therefore, the rapid differentiation between NTM and Mycobacterium tuberculosis complex is crucial for timely therapeutic decisions. This study evaluates a new rapid molecular assay, Standard M10 MTB/NTM, designed to detect MTB, NTM, or co-detection in Mycobacteria Growth Indicator Tube cultures from different biological matrices. The assay was validated using 100 positive and 50 negative liquid mycobacteria cultures, already confirmed by specific real-time PCR and Sanger sequencing. Following optimization of assay conditions for culture sample processing and assessment of potential interference, Standard M10 demonstrated excellent sample stability, high specificity, and good sensitivity, identifying all 50 MTB and 49 NTM samples. Some limitations included the non-detection of M. celatum in one case and false positive results (MTB co-infection) in two NTB cases. Nevertheless, overall, the adoption of this test could be considered for laboratory management to enable rapid and effective sample targeting for subsequent diagnostic evaluation and treatment decision-making.","PeriodicalId":19758,"journal":{"name":"Pathogens","volume":"14 6","pages":""},"PeriodicalIF":3.3,"publicationDate":"2025-05-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12195744/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144485435","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Prospective Analysis of Clinicopathologic Correlates of At-Home Feline Infectious Peritonitis Treatment Using GS-441524. GS-441524在家中治疗猫感染性腹膜炎临床病理相关性的前瞻性分析。

IF 3.3 3区医学

Pathogens Pub Date : 2025-05-21 DOI: 10.3390/pathogens14050507

Kelly Larson, Emma Hart, Rosa Negash, Wendy Novicoff, Nicole Jacque, Samantha Evans

{"title":"Prospective Analysis of Clinicopathologic Correlates of At-Home Feline Infectious Peritonitis Treatment Using GS-441524.","authors":"Kelly Larson, Emma Hart, Rosa Negash, Wendy Novicoff, Nicole Jacque, Samantha Evans","doi":"10.3390/pathogens14050507","DOIUrl":"10.3390/pathogens14050507","url":null,"abstract":"Feline Infectious Peritonitis (FIP) is caused by a systemic feline coronavirus (FCoV). Prior to June 2024, compounded FIP treatment was unavailable for prescription by veterinarians in the United States, leading to many cat owners obtaining treatment through unlicensed \"black market\" sources. We hypothesized that clinicopathologic data could provide insight on prognostic indicators for the treatment of FIP with GS-441524. This study used data gathered via surveys from 126 cat owners who used \"black market\" GS-441524 for their cats. We compared bloodwork parameters over twelve weeks of treatment. None of the clinicopathologic correlates, when analyzed via two-sample t-tests, produced statistically significant results between cured, deceased, and relapsed groups. Within cats considered cured, it was observed that hematocrit (HCT) and white blood cell (WBC) values were within normal limits by the 2-6-week period. Cats who died during the study had lower HCT and higher WBC values within the 2-6-week period. Trends were also seen in A/G and total bilirubin (T-BIL), with deceased patients showing a higher A/G ratio and lower value than those in the cured group. Overall, these data demonstrate a lack of traditional clinicopathologic parameters which are consistently predictive of FIP therapy success. Other predictors of outcome with antiviral therapy should be pursued.","PeriodicalId":19758,"journal":{"name":"Pathogens","volume":"14 5","pages":""},"PeriodicalIF":3.3,"publicationDate":"2025-05-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12114938/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144160916","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A Complete Transfer Learning-Based Pipeline for Discriminating Between Select Pathogenic Yeasts from Microscopy Photographs. 从显微镜照片中鉴别病原酵母菌的完整迁移学习管道。

IF 3.3 3区医学

Pathogens Pub Date : 2025-05-21 DOI: 10.3390/pathogens14050504

Ryan A Parker, Danielle S Hannagan, Jan H Strydom, Christopher J Boon, Jessica Fussell, Chelbie A Mitchell, Katie L Moerschel, Aura G Valter-Franco, Christopher T Cornelison

{"title":"A Complete Transfer Learning-Based Pipeline for Discriminating Between Select Pathogenic Yeasts from Microscopy Photographs.","authors":"Ryan A Parker, Danielle S Hannagan, Jan H Strydom, Christopher J Boon, Jessica Fussell, Chelbie A Mitchell, Katie L Moerschel, Aura G Valter-Franco, Christopher T Cornelison","doi":"10.3390/pathogens14050504","DOIUrl":"10.3390/pathogens14050504","url":null,"abstract":"Pathogenic yeasts are an increasing concern in healthcare, with species like Candida auris often displaying drug resistance and causing high mortality in immunocompromised patients. The need for rapid and accessible diagnostic methods for accurate yeast identification is critical, especially in resource-limited settings. This study presents a convolutional neural network (CNN)-based approach for classifying pathogenic yeast species from microscopy images. Using transfer learning, we trained the model to identify six yeast species from simple micrographs, achieving high classification accuracy (93.91% at the patch level, 99.09% at the whole image level) and low misclassification rates across species, with the best performing model. Our pipeline offers a streamlined, cost-effective diagnostic tool for yeast identification, enabling faster response times in clinical environments and reducing reliance on costly and complex molecular methods.","PeriodicalId":19758,"journal":{"name":"Pathogens","volume":"14 5","pages":""},"PeriodicalIF":3.3,"publicationDate":"2025-05-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12114329/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144160778","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0