Pathogens最新文献

{"title":"Seroprevalence and Risk Factors for Hepatitis B, HIV, and Syphilis Among Survivors of Sexual Violence in the Eastern Cape Province, South Africa.","authors":"Oladele Vincent Adeniyi, Charity Masilela, Oyewole Christopher Durojaiye","doi":"10.3390/pathogens14030209","DOIUrl":"10.3390/pathogens14030209","url":null,"abstract":"<p><p>Understanding the prevalence of hepatitis B (HBV), human immunodeficiency virus (HIV), and syphilis among survivors of sexual violence in South Africa is crucial for guiding targeted healthcare interventions, despite the limited available data. This study aimed to investigate the prevalence of these infections and their associated risk factors in survivors from the Eastern Cape Province, South Africa. This retrospective cross-sectional study reviewed 1957 medical records of survivors of sexual violence who received care at two large healthcare facilities in the Eastern Cape Province of South Africa between January 2019 and December 2020. All survivors were screened for HBV, HIV, and syphilis infections. Logistic regression analysis was used to identify factors associated with HIV and syphilis infections. The overall seroprevalence rates for HBV, syphilis, and HIV were 0.7%, 4.9%, and 17.3%, respectively. Six individuals were co-infected with at least two of these infections. Predictors for HIV infection included age (age < 16: adjusted odds ratio [aOR] 0.05; 95% confidence interval [CI], 0.03-0.08 and ages 16-25: aOR 0.45; 95% CI, 0.34-0.59) and black race (aOR 4.78; 95% CI, 1.09-20.88). Predictors for syphilis infection were age (age < 16: aOR 0.05; 95% CI, 0.02-0.15 and ages 16-25: aOR 0.41; 95% CI, 0.25-0.66) and residing in an urban area (aOR 0.23; 95% CI, 0.10-0.50). Survivors of sexual violence are at increased risk of HBV, HIV, and syphilis. Urgent measures are needed to provide comprehensive screening, treatment, prevention, and education to address this critical public health issue.</p>","PeriodicalId":19758,"journal":{"name":"Pathogens","volume":"14 3","pages":""},"PeriodicalIF":3.3,"publicationDate":"2025-02-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11944787/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143730866","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Computational and Population-Based HLA Permissiveness to HIV Drug Resistance-Associated Mutations.","authors":"Rizwan Mahmud, Zoë Krullaars, Jolieke van Osch, David Rickett, Zabrina L Brumme, Kathryn S Hensley, Casper Rokx, Rob A Gruters, Jeroen J A van Kampen, Thibault Mesplède","doi":"10.3390/pathogens14030207","DOIUrl":"10.3390/pathogens14030207","url":null,"abstract":"<p><p>The presentation of HIV peptides by the human leukocyte antigen (HLA) complex to CD8+ cytotoxic T-cells (CTLs) is critical to limit viral pathogenesis. HIV can mutate to evade HLA-restricted CTL responses and resist antiretroviral drugs, raising questions about how it balances these evolutionary pressures. Here, we used a computational approach to assess how drug resistance-associated mutations (RAMs) affect the binding of HIV-1 subtype B or C peptides to the most prevalent HLA alleles in US, European, and South African populations. We predict RAMs that may be favored in certain populations and report the under-representation of Y181C in people expressing HLA-B*57:01. This finding agreed with our computational predictions when Y181C was at the major anchor site P2, suggesting the potential relevance of our approach. Overall, our findings lay out a conceptual framework to study the implications of HLA alleles on the emergence of HIV RAMs at the individual and population levels.</p>","PeriodicalId":19758,"journal":{"name":"Pathogens","volume":"14 3","pages":""},"PeriodicalIF":3.3,"publicationDate":"2025-02-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11944876/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143731007","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Whole-Genome Sequencing of Extended-Spectrum β-Lactamase-Producing <i>Klebsiella pneumoniae</i> Isolated from Human Bloodstream Infections.","authors":"Carolina Sabença, Rani Rivière, Eliana Costa, Sara Sousa, Manuela Caniça, Vanessa Silva, Gilberto Igrejas, Carmen Torres, Patrícia Poeta","doi":"10.3390/pathogens14030205","DOIUrl":"10.3390/pathogens14030205","url":null,"abstract":"<p><p><i>Klebsiella pneumoniae</i> is a Gram-negative bacterium commonly associated with bloodstream infections (BSIs), which can lead to severe clinical outcomes, especially in immunocompromised individuals or patients with underlying health conditions. The increasing prevalence of <i>K. pneumoniae</i> that produces extended-spectrum β-lactamases (ESBL) poses a significant challenge for treatment and infection control, necessitating a swift diagnostic approach and tailored antimicrobial therapy to improve patient outcomes. A total of 32 <i>K. pneumoniae</i> isolates were recovered from BSIs from December 2021 to August 2022. Whole-genome sequencing (WGS) was performed on the 14 ESBL-producing isolates. All ESBL isolates carried the <i>bla</i>_CTX-M-15 gene, together with other β-lactamase-encoding genes (<i>bla</i>_TEM-1, <i>bla</i>_SHV-28, <i>bla</i>_SHV-26, or <i>bla</i>_OXA-1). Three of the isolates also carried the <i>bla</i>_KPC-3 gene. Resistance genes to quinolones, sulfonamides, tetracycline, aminoglycosides, and chloramphenicol were also detected. We can conclude that the presence of ESBL-producing isolates among <i>K. pneumoniae</i> of BSIs raises concerns, since these enzymes limit the available treatment options, and future research must include studies on alternative therapies for dealing with resistant bacterial infections and developing new approaches to disease treatment.</p>","PeriodicalId":19758,"journal":{"name":"Pathogens","volume":"14 3","pages":""},"PeriodicalIF":3.3,"publicationDate":"2025-02-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11944550/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143730971","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of Live and Peptide-Based Antimicrobiota Vaccines on <i>Ixodes ricinus</i> Fitness, Microbiota, and Acquisition of Tick-Borne Pathogens.","authors":"Apolline Maitre, Lourdes Mateos-Hernandez, Myriam Kratou, Natalia Egri, Jennifer Maye, Manel Juan, Adnan Hodžić, Dasiel Obregón, Lianet Abuin-Denis, Elianne Piloto-Sardinas, Andrea C Fogaça, Alejandro Cabezas-Cruz","doi":"10.3390/pathogens14030206","DOIUrl":"10.3390/pathogens14030206","url":null,"abstract":"<p><p>This study explored the effects of antimicrobiota vaccines on the acquisition of <i>Borrelia</i> and <i>Rickettsia</i>, and on the microbiota composition of <i>Ixodes ricinus</i> ticks. Using a murine model, we investigated the immunological responses to live <i>Staphylococcus epidermidis</i> and multi-antigenic peptide (MAP) vaccines. Immunized mice were infected with either <i>Borrelia afzelii</i> or <i>Rickettsia helvetica</i>, and subsequently infested with pathogen-free <i>I. ricinus</i> nymphs. We monitored the tick feeding behavior, survival rates, and infection levels. Additionally, we employed comprehensive microbiota analyses, including the alpha and beta diversity assessments and microbial co-occurrence network construction. Our results indicate that both live <i>S. epidermidis</i> and MAP vaccines elicited significant antibody responses in mice, with notable bactericidal effects against <i>S. epidermidis</i>. The vaccination altered the feeding patterns and fitness of the ticks, with the Live vaccine group showing a higher weight and faster feeding time. Microbiota analysis revealed significant shifts in the beta diversity between vaccine groups, with distinct microbial networks and taxa abundances observed. Notably, the MAP vaccine group exhibited a more robust and complex network structure, while the Live vaccine group demonstrated resilience to microbial perturbations. However, the effects of antimicrobiota vaccination on <i>Borrelia</i> acquisition appeared taxon-dependent, as inferred from our results and previous findings on microbiota-driven pathogen refractoriness. <i>Staphylococcus</i>-based vaccines altered the microbiota composition but had no effect on <i>B. afzelii</i> infection, and yielded inconclusive results for <i>R. helvetica</i>. In contrast, previous studies suggest that <i>E. coli</i>-based microbiota modulation can induce a pathogen-refractory state, highlighting the importance of both bacterial species and peptide selection in shaping microbiota-driven pathogen susceptibility. However, a direct comparison under identical experimental conditions across multiple taxa is required to confirm this taxon-specific effect. These findings suggest that antimicrobiota vaccination influences tick fitness and microbiota assembly, but its effects on pathogen transmission depend on the bacterial taxon targeted and the selected peptide epitopes. This research provides insights into the need for strategic bacterial taxon selection to enhance vaccine efficacy in controlling tick-borne diseases.</p>","PeriodicalId":19758,"journal":{"name":"Pathogens","volume":"14 3","pages":""},"PeriodicalIF":3.3,"publicationDate":"2025-02-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11945021/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143731096","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Deciphering Host-Pathogen Interactions: Role of <i>Cryptosporidium</i> in Tumorigenesis.","authors":"Shakeel Hussain, Qurrat Ul Ain, Muhammad Aamir, Khalid M Alsyaad, Ahmed Ezzat Ahmed, Jude G Zakai, Haytham Ahmed Zakai, Yongzhong Hou","doi":"10.3390/pathogens14030208","DOIUrl":"10.3390/pathogens14030208","url":null,"abstract":"<p><p><i>Cryptosporidium</i>, a protozoan parasite affecting the gastrointestinal system, is primarily known for causing diarrhea, especially in those with weakened immune systems. However, there is increasingly persuasive evidence that it may be directly involved in tumorigenesis. This review examines some of the potential mechanisms through which <i>Cryptosporidium</i> infections can induce cancer, specifically chronic inflammation, manipulation of the immune system, and alteration of cell signaling pathways. Persistent inflammation with immune system changes due to chronic infection, particularly among immunocompromised hosts, leads to a microenvironment that facilitates tumorigenesis. <i>Cryptosporidium</i> manipulates important cellular pathways such as PI3K, NF-κB, Wnt, and p38/MAPK to promote cell survival, regulate immune responses, and foster tissue remodeling, all of which contribute to a tumor-friendly microenvironment. Moreover, <i>Cryptosporidium</i> virulence factors such as ROP1, sPLA2, and microRNAs disrupt host cellular stability and significantly alter host cellular gene expression, which also exacerbates inflammation and tissue damage. Epidemiological data have indicated higher rates of <i>Cryptosporidium</i> infection in cancer patients, especially patients with gastrointestinal cancers. This, among other observations, raises the possibility that the infection may be connected to cancer progression. In animal models, especially studies with <i>C. parvum</i>-challenged rodents, chronic inflammation, immune repression, and genetic mutations related to neoplasia have been reported. While this has provided us with valuable information, we still have a long way to go to fully understand the long-term ramifications of <i>Cryptosporidium</i> infection. These cover aspects such as the contribution of latent infections and the genetic diversity of <i>Cryptosporidium</i> strains in cancer. Further investigation is urgently needed to understand the molecular processes by which <i>Cryptosporidium</i> might contribute to carcinogenesis and explore potential strategies for therapy and prevention especially among immunocompromised populations.</p>","PeriodicalId":19758,"journal":{"name":"Pathogens","volume":"14 3","pages":""},"PeriodicalIF":3.3,"publicationDate":"2025-02-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11946830/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143731023","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"First Molecular Detection of <i>Rickettsia conorii</i> and <i>Rickettsia helvetica</i> in Ticks from Dogs in Luxembourg.","authors":"Guilherme Moreira, Rafaela S S Moreira, Floriane André das Neves, Vanessa Swiontek, Patrícia F Barradas, Sara Gomes-Gonçalves, João R Mesquita","doi":"10.3390/pathogens14020204","DOIUrl":"10.3390/pathogens14020204","url":null,"abstract":"<p><p>Vector-borne diseases, particularly those caused by <i>Rickettsia</i> species, pose a significant public health threat in Europe. Despite extensive research on tick-borne pathogens in various European countries, Luxembourg has yet not been studied for <i>Rickettsia</i> spp. in ticks infesting domestic animals. This study aimed to fill this gap by investigating the presence of <i>Rickettsia</i> spp. in <i>Ixodes ricinus</i> ticks collected from domestic dogs in Luxembourg between April 2023 and April 2024. A total of 61 ticks were examined using molecular techniques, including PCR amplification of the outer membrane protein B (<i>ompB</i>), outer membrane protein A (<i>ompA</i>), and citrate synthase (<i>gltA</i>) genes. Results revealed the presence of <i>R. helvetica</i> and <i>R. conorii</i> subsp. <i>raoultii</i>, with 4.9% of ticks testing positive for <i>Rickettsia</i> spp. Phylogenetic analysis confirmed the high genetic identity of the sequences obtained with previously described strains from Europe and Asia. This study highlights the potential risk of emerging tick-borne diseases in Luxembourg and emphasizes the need for ongoing surveillance to better understand the spread of <i>Rickettsia</i> spp. in Europe, particularly as climate change may facilitate the expansion of tick populations and their associated pathogens.</p>","PeriodicalId":19758,"journal":{"name":"Pathogens","volume":"14 2","pages":""},"PeriodicalIF":3.3,"publicationDate":"2025-02-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11858352/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143503024","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Fatal Toxoplasmosis in Red Kangaroos (<i>Macropus rufus</i>) in East China.","authors":"Haiyan Gong, Quan Wang, Yinghong Jin, Suoping Qiu, Zhaoguo Chen, Xiangan Han, Zongyan Chen, Wei Jiang","doi":"10.3390/pathogens14020202","DOIUrl":"10.3390/pathogens14020202","url":null,"abstract":"<p><strong>Background: </strong>As a ubiquitous apicomplexan parasite, <i>Toxoplasma gondii</i> causes huge economic losses and poses a great threat to the health of animals, including humans, worldwide. In some kangaroo species, <i>T. gondii</i> can be fatal. To date, little information is available on <i>T. gondii</i> infection in the red kangaroos in east China. At a zoo in east China, thirteen red kangaroos consecutively developed clinical signs from July to November 2016, resulting in the deaths of seven, three of which were analyzed in this study.</p><p><strong>Methods: </strong>In the present study, ascitic fluid, blood and samples from the brain, heart, liver, spleen, lung, kidney, and mesenteric lymph nodes of three dead red kangaroos were collected. The pathogen was explored through microscopic observation, nested PCR, immunofluorescence antibody test (IFAT), hematoxylin-eosin (HE) staining, and immunohistochemistry (IHC) staining, respectively. Meanwhile, the potential source of the infection was also investigated by testing the blood of stray cats in the zoo for <i>T. gondii</i> using nested PCR.</p><p><strong>Results: </strong>Three dead red kangaroos were subjected to a necropsy, and organisms resembling <i>T. gondii</i> were detected in their ascitic fluids under microscope. This infection was further confirmed by a nested PCR assay, which resulted in a successful amplification and sequencing of the 433 bp fragment of the <i>T. gondii</i> 5.8S rRNA gene in all the dissected tissues, including heart, liver, spleen, lung, kidney, lymph nodes, cecum, and brain, as well as in body fluids (blood and ascitic fluid). Furthermore, the tachyzoites were observed in the heart, liver, spleen, lymph nodes, cecum, and brain through IFAT and HE staining. Administration of classic drugs (sulfadiazine and pyrimethamine) against <i>T. gondii</i> significantly alleviated the clinical signs of the sick kangaroos. The possible source of this infection was traced to a native stray cat, as <i>T. gondii</i> DNA was detected in its blood.</p><p><strong>Conclusions: </strong>In the present study, lethal <i>T. gondii</i> infection in red kangaroos has been described for the first time in east China, highlighting the necessity and urgency for close and long-term surveillance of this parasite infection in captive animals. The same strain of <i>T. gondii</i> detected in kangaroos as that found in stray cats wandering in the same area emphasizes the importance of controlling stray cat populations to mitigate the risk of <i>Toxoplasma</i> transmission to other animals.</p>","PeriodicalId":19758,"journal":{"name":"Pathogens","volume":"14 2","pages":""},"PeriodicalIF":3.3,"publicationDate":"2025-02-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11858611/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143503119","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Contrasting Effect of Curcumin on Hepatitis B Virus Replication According to the Hepatoma Cell Line.","authors":"María Mercedes Elizalde, Pedro Fuentes, Diego Chiappetta, Diego Martín Flichman","doi":"10.3390/pathogens14020203","DOIUrl":"10.3390/pathogens14020203","url":null,"abstract":"<p><p>In recent decades, considerable advances have been achieved in the treatment of chronic hepatitis B. However, the currently available drugs have shortcomings. In this context, several natural compounds have been proposed as potential agents to improve either the outcome of antiviral treatment or the progression of chronic infection, with curcumin being one of the most evaluated compounds due to its pleiotropic antiviral activity. The aim of this study was to characterize the effect and mechanism of curcumin on hepatitis B virus (HBV) replication in two different experimental models. Treatment of HepG22.15 and HBV-transfected Huh7 cells with curcumin revealed that the phytochemical differentially modulated HBV replication in both cell lines. In HepG22.15 cells, the addition of curcumin had no effect on viral DNA, pregenomic RNA (pgRNA), and e antigen (HBeAg) levels, while it decreased Precore RNA and s antigen (HBsAg) levels. Conversely, in Huh-7 cells, curcumin significantly increased viral progeny more than tenfold, as well as HBV RNAs and viral antigens. Furthermore, the analysis of the cellular mechanisms associated with the modulation of viral replication revealed that in Huh-7 cells, curcumin-induced cell cycle arrest in the G2/M phase and the modulation of genes involved in proliferation, cell cycle progression, and apoptosis, whereas no changes in cell cycle progression and gene expression were observed in HepG22.15 cells. In conclusion, curcumin elicits a differential cellular response in two hepatoma cell lines, which, in the case of Huh-7 cells, would provide an optimal cellular setting that enhances HBV replication. Therefore, the antiviral effect of this phytochemical remains controversial.</p>","PeriodicalId":19758,"journal":{"name":"Pathogens","volume":"14 2","pages":""},"PeriodicalIF":3.3,"publicationDate":"2025-02-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11858270/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143502843","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Canine Adenoviruses in Wildlife: Role in At-Risk Species Conservation and Interface with Domestic Animals.","authors":"Andrea Balboni, Martina Magliocca, Lorenza Urbani, Mara Battilani","doi":"10.3390/pathogens14020200","DOIUrl":"10.3390/pathogens14020200","url":null,"abstract":"<p><p>Canine adenovirus type 1 (CAdV-1) and type 2 (CAdV-2) are well known pathogens of domestic dogs but are little investigated in wild animals. The few available studies about CAdV-1 in wild animals show that it circulates in various species and that transmission of the virus in the interface between wildlife and domestic animals is a frequent event. Furthermore, wild animals are usually subject to asymptomatic infections, but cases of serious and fatal diseases have been documented, with possible effects on the conservation of the species. In contrast, CAdV-2 infection was reported only recently and sporadically in some wild animals, with few data regarding its pathogenic role in these species. However, the real prevalence of these viruses in wildlife is still uncertain due to the use of serological tests that are largely unable to distinguish antibodies against CAdV-1 and CAdV-2. This review, reporting all the data currently available on CAdV-1 and CAdV-2 infection in wild animals, highlights the importance of these pathogens for wildlife conservation and their role in the potential transmission of the infection to domestic dogs.</p>","PeriodicalId":19758,"journal":{"name":"Pathogens","volume":"14 2","pages":""},"PeriodicalIF":3.3,"publicationDate":"2025-02-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11858118/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143502617","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Characterization of Fungal Pathogens Causing Blueberry Fruit Rot Disease in China.","authors":"Yueyan Zhou, Wei Zhang, Linna Wu, Pengzhao Chen, Xinghong Li, Guangqin Wen, Khanobporn Tangtrakulwanich, Kandawatte Wedaralalage Thilini Chethana, Fatimah Al-Otibi, Kevin D Hyde, Jiye Yan","doi":"10.3390/pathogens14020201","DOIUrl":"10.3390/pathogens14020201","url":null,"abstract":"<p><p>Blueberry has been a burgeoning fruit in China in recent years, but its perishable nature places a constant strain on industrial development. To determine the pathogens infecting blueberry fruits, diseased samples were collected from Guizhou and Fujian Provinces. Isolates from the samples were identified by morphological characterization and phylogenetic analyses. Pathogenicity assays were conducted on fresh blueberry fruits using spore suspensions. Sixteen isolates were identified as seven species, namely, <i>Botryosphaeria dothidea</i>, <i>Botrytis cinerea</i>, <i>Cladosporium guizhouense</i>, <i>Colletotrichum fioriniae</i>, <i>Diaporthe anacardii</i>, <i>Fusarium annulatum</i>, and <i>Neopestalotiopsis surinamensis</i>, and their pathogenicity on blueberry fruits were confirmed following Koch's postulates. The current study reported <i>Cladosporium guizhouense</i>, <i>Fusarium annulatum</i>, and <i>Neopestalotiopsis surinamensis</i> for the first time on blueberry. The study (1) demonstrated that fruit rot disease results from a mixed infection of multiple pathogens; and (2) expanded the understanding of causal agents of blueberry fruit rot during the growth stage, highlighting their potential as latent pathogens that contribute to post-harvest losses. Relevant results provide a reference for the etiological research and disease management in blueberry fruit diseases.</p>","PeriodicalId":19758,"journal":{"name":"Pathogens","volume":"14 2","pages":""},"PeriodicalIF":3.3,"publicationDate":"2025-02-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11858039/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143502560","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}