EmsB Microsatellite Analysis of Echinococcus multilocularis Specimens Isolated from Belgian Patients with Alveolar Echinococcosis and from Animal Hosts.

IF 3.3 3区 医学 Q2 MICROBIOLOGY
Sabrina Egrek, Jenny Knapp, Rosalie Sacheli, Khalid El Moussaoui, Philippe Léonard, Eva Larranaga Lapique, Laurence Millon, Sara Engelskirchen, Olivier Detry, Annick Linden, Marie-Pierre Hayette
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Abstract

Alveolar echinococcosis (AE), caused by Echinococcus multilocularis (E. multilocularis), is a severe parasitic zoonosis that is potentially fatal for humans. The parasite is primarily transmitted by wildlife, with red foxes acting as definitive hosts and rodents as intermediate hosts, while humans can become accidental but dead-end hosts. The aim of this study is to use EmsB typing on E. multilocularis isolates from human AE cases and local animals such as foxes and rodents. In this study, retrospective EmsB typing was performed on 39 samples, including 11 tissue samples from 10 patients, 18 fecal swabs from foxes, and 10 tissue samples from rodents. A dendrogram was created to determine the EmsB profiles present. The results showed that all the rodent samples were associated with the EmsB P1 profile (10/10), while the human and fox samples shared the EmsB profile P1 (5/11 humans and 8/18 foxes), a profile near P4 (2/11 humans and 3 foxes), and a profile near P8 (1/11 humans and 1/18 foxes). The study demonstrates that the same EmsB profiles circulate among humans and animals, confirming that wildlife reservoirs play a key role in transmission.

比利时肺泡棘球蚴病和动物宿主多房棘球蚴标本的EmsB微卫星分析。
肺泡棘球蚴病(AE)是由多房棘球绦虫(E. multilocularis)引起的一种严重的寄生虫性人畜共患病,对人类具有潜在的致命性。这种寄生虫主要通过野生动物传播,红狐是最终宿主,啮齿动物是中间宿主,而人类可能成为偶然的死角宿主。本研究的目的是利用EmsB分型方法对从人类AE病例和当地动物(如狐狸和啮齿动物)分离的多室e分离株进行分型。本研究对39份样本进行了回顾性EmsB分型,其中包括来自10名患者的11份组织样本、来自狐狸的18份粪便拭子和来自啮齿动物的10份组织样本。创建了一个树状图来确定存在的EmsB配置文件。结果表明,所有啮齿动物样本均具有EmsB P1基因(10/10),而人类和狐狸样本具有EmsB P1基因(5/11人和8/18狐狸)、P4基因(2/11人和3只狐狸)和P8基因(1/11人和1/18狐狸)。该研究表明,相同的EmsB谱在人类和动物之间传播,证实野生宿主在传播中起关键作用。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Pathogens
Pathogens Medicine-Immunology and Allergy
CiteScore
6.40
自引率
8.10%
发文量
1285
审稿时长
17.75 days
期刊介绍: Pathogens (ISSN 2076-0817) publishes reviews, regular research papers and short notes on all aspects of pathogens and pathogen-host interactions. There is no restriction on the length of the papers. Our aim is to encourage scientists to publish their experimental and theoretical research in as much detail as possible. Full experimental and/or methodical details must be provided for research articles.
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