Characterization of Microbial Growth, Pathogen Presence, and Histamine Accumulation in Chilled Rainbow Trout and Mackerel Samples Collected from Romanian Markets.

IF 3.3 3区医学 Q2 MICROBIOLOGY

Pathogens Pub Date : 2025-06-11 DOI:10.3390/pathogens14060580

Vida Silviu, Alexandra Tabaran, Oana Lucia Crişan Reget, Mihaela Niculina Duma, Luciana Cătălina Panait, Sorin Daniel Dan

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引用次数: 0

Abstract

This study aimed to evaluate microbial growth, pathogen presence, and histamine production in rainbow trout and mackerel stored on flaked ice over their shelf life. A total of 72 fish samples (rainbow trout and mackerel) were analyzed across four storage intervals (day 1, 3, 9, 12/11) on flaked ice. TVC increased from 2.59 to 5.04 log cfu/g in rainbow trout and from 3.18 to 4.88 log cfu/g in mackerel over the storage period. Significant increases were observed in Pseudomonas, Aeromonas, and Enterobacteriaceae populations, especially after the ninth day. Microbial identification revealed spoilage-associated bacteria, such as Pseudomonas fluorescens and Aeromonas salmonicida, as well as opportunistic pathogens, including Francisella tularensis, Yersinia spp., and Chromobacterium violaceum. Histamine levels rose with storage time but remained below toxic thresholds (<200 mg/kg), peaking at 1.56 mg/kg in trout and 1.87 mg/kg in mackerel. A strong positive correlation was found between TVC and histamine levels (Pearson's r = 0.85 for trout, 0.82 for mackerel). Proper hygiene and storage are crucial, and consumption is recommended before day 9 of storage on flaked ice. Hygiene measures remain essential to minimize contamination risks and preserve product safety.

查看原文本刊更多论文

从罗马尼亚市场收集的冷冻虹鳟鱼和鲭鱼样品中微生物生长、病原体存在和组胺积累的特征。

本研究旨在评估虹鳟鱼和鲭鱼在冰片储存期间的微生物生长、病原体存在和组胺产生。在4个储存间隔（第1、3、9、12/11天）的薄片冰上，共分析了72种鱼类样本（虹鳟鱼和鲭鱼）。贮藏期间，虹鳟鱼的TVC从2.59 log cfu/g增加到5.04 log cfu/g，鲐鱼的TVC从3.18 log cfu/g增加到4.88 log cfu/g。假单胞菌、气单胞菌和肠杆菌科菌群显著增加，特别是在第9天之后。微生物鉴定发现了与腐败相关的细菌，如荧光假单胞菌和沙门氏菌气单胞菌，以及机会致病菌，包括土拉菌弗朗西斯菌、耶尔森氏菌和紫罗兰色杆菌。组胺含量随储存时间延长而上升，但仍低于中毒阈值(

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来源期刊

Pathogens Medicine-Immunology and Allergy

CiteScore

6.40

自引率

8.10%

发文量

1285

审稿时长

17.75 days

期刊介绍： Pathogens (ISSN 2076-0817) publishes reviews, regular research papers and short notes on all aspects of pathogens and pathogen-host interactions. There is no restriction on the length of the papers. Our aim is to encourage scientists to publish their experimental and theoretical research in as much detail as possible. Full experimental and/or methodical details must be provided for research articles.