Open Biology最新文献_第4页

NudC moonlights in ribosome biogenesis and homeostasis in polyploid cells of Drosophila melanogaster. NudC在黑腹果蝇多倍体细胞核糖体生物发生和体内平衡中起着重要作用。

IF 3.6 3区生物学

Open Biology Pub Date : 2026-06-03 DOI: 10.1098/rsob.260066

Duoduo Shi, Yuko Shimada-Niwa, Naoki Okamoto, Akira Nakamura, Yuya Ohhara, Yuichi Shichino, Shintaro Iwasaki, Wei Sun, Ryusuke Niwa

{"title":"NudC moonlights in ribosome biogenesis and homeostasis in polyploid cells of Drosophila melanogaster.","authors":"Duoduo Shi, Yuko Shimada-Niwa, Naoki Okamoto, Akira Nakamura, Yuya Ohhara, Yuichi Shichino, Shintaro Iwasaki, Wei Sun, Ryusuke Niwa","doi":"10.1098/rsob.260066","DOIUrl":"10.1098/rsob.260066","url":null,"abstract":"Ribosomes, the cellular machinery responsible for protein synthesis, are fundamental across all kingdoms of life. Disruption of ribosome biogenesis (RiBi) can cause severe ribosomopathies, underscoring the need for precise regulatory mechanisms. In this study, we identified a role for the gene nuclear distribution C, dynein complex regulator (NudC) in RiBi within polyploid cells of Drosophila melanogaster larvae. Depletion of NudC in polyploid salivary gland cells led to a significant reduction in ribosome abundance, accompanied by the loss of ribosome-binding sites on the rough endoplasmic reticulum and impaired translation. These defects are linked to decreased ribosomal RNA levels. Notably, NudC knockdown also triggered a homeostatic response, characterized by increased transcription and translation of ribosome biogenesis factors and ribosomal proteins. This response is similar to that observed in cells with defective ribosomal activity, suggesting that the ribosomal impairment triggers transcriptional feedback to maintain ribosome function. Meanwhile, NudC-deficient cells exhibited chromosome abnormalities, JNK signalling activation and autophagy-resembling defects from ribosome dysfunction. Finally, our findings suggest that the role of NudC in RiBi is independent of its established function in dynein regulation, indicating its moonlighting role in RiBi. Together, these results uncover a new, fundamental function for NudC in maintaining RiBi and homeostasis in polyploid cells.","PeriodicalId":19629,"journal":{"name":"Open Biology","volume":"16 6","pages":""},"PeriodicalIF":3.6,"publicationDate":"2026-06-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148150741","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Pan-resistome and genomic plasticity analysis of antibiotic resistance in Aeromonas: new vaccine targets for A. caviae, A. veronii and A. hydrophila through reverse vaccinology. 气单胞菌抗生素耐药的泛抵抗组和基因组可塑性分析：通过反向疫苗学研究对A. caviae、A. veronii和A. hydroophila的新疫苗靶点。

IF 3.1 3区生物学

Open Biology Pub Date : 2026-05-27 DOI: 10.1098/rsob.250423

Caio Luigi Antunes Moura Tristão, Andrei Giacchetto Felice, Ligia Carolina da Silva Prado, Mateus Mattiuzi, Gisele Veneron, Pedro Henrique Marques, Siomar de Castro Soares

{"title":"Pan-resistome and genomic plasticity analysis of antibiotic resistance in Aeromonas: new vaccine targets for A. caviae, A. veronii and A. hydrophila through reverse vaccinology.","authors":"Caio Luigi Antunes Moura Tristão, Andrei Giacchetto Felice, Ligia Carolina da Silva Prado, Mateus Mattiuzi, Gisele Veneron, Pedro Henrique Marques, Siomar de Castro Soares","doi":"10.1098/rsob.250423","DOIUrl":"10.1098/rsob.250423","url":null,"abstract":"Aeromonas species are globally significant pathogens. However, the mechanisms driving their antimicrobial resistance patterns remain unclear. This study addresses the spread of resistance genes in the Aeromonas genus through a large-scale genomic analysis of all complete Aeromonas genomes in the RefSeq database. The emergence of next-generation genomic sequencing enabled the sequencing, assembling and annotation of numerous genomes with a description and characterization of the genomic plasticity and the pan-resistome, through bioinformatics programmes, of each species in the Aeromonas genus, and revealed species-specific patterns of resistance determinants. Leveraging these genomic insights, we applied a reverse vaccinology approach with a subtractive genomic workflow to select novel in silico vaccine targets for the three main pathogens: A. veronii, A. hydrophila and A. caviae. These protein candidates offer a potential alternative to prevent the spread of antibiotic resistance genes. Our findings underscore that continuous genomic surveillance is essential for monitoring established and emerging pathogens. While further in vitro and in vivo validation is pending, this work provides a robust framework for understanding Aeromonas resistance and developing new strategies to protect public and environmental health.","PeriodicalId":19629,"journal":{"name":"Open Biology","volume":"16 5","pages":""},"PeriodicalIF":3.1,"publicationDate":"2026-05-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148033880","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Investigating how changes in the levels of kinesins impact neuronal health in Drosophila and human iPSC-derived neurons AD model. 在果蝇和人类ipsc衍生的神经元AD模型中，研究运动蛋白水平的变化如何影响神经元健康。

IF 3.1 3区生物学

Open Biology Pub Date : 2026-05-13 DOI: 10.1098/rsob.250319

Deepthy Francis, Francesco Paonessa, Victoria L Hewitt, Maria Southall, Isabel Peset, Alexander J Whitworth, Frederick J Livesey, Caroline C G Fabre, Isabel M Palacios

{"title":"Investigating how changes in the levels of kinesins impact neuronal health in Drosophila and human iPSC-derived neurons AD model.","authors":"Deepthy Francis, Francesco Paonessa, Victoria L Hewitt, Maria Southall, Isabel Peset, Alexander J Whitworth, Frederick J Livesey, Caroline C G Fabre, Isabel M Palacios","doi":"10.1098/rsob.250319","DOIUrl":"10.1098/rsob.250319","url":null,"abstract":"Alzheimer's disease (AD) is the leading cause of dementia and the most common neurodegenerative disorder. Understanding the molecular pathology of AD may help identify new ways to reduce neuronal damage. In the past decades, Drosophila has become a powerful tool in modelling mechanisms underlying human diseases. Here, we investigate how the expression of the human 42-residue β-amyloid (Aβ) carrying the E22G pathogenic 'Arctic' mutation (Aβ42Arc) affects axonal health and behaviour in Drosophila. We find that Aβ42Arc flies present aberrant neurons, with altered axonal transport of mitochondria and aberrant terminal boutons at neuromuscular junctions. We demonstrate that the motor proteins kinesin-1 and kinesin-3 are essential for the correct development of neurons in Drosophila larvae and in human induced pluripotent stem cell-derived cortical neurons. We then show that the overexpression of kinesin-1 or kinesin-3 restores the correct number and morphology of boutons in Aβ42Arc-expressing neurons and rescues neuronal function measured by negative geotaxis locomotor behavioural assay. We therefore provide new evidence towards understanding the mechanisms of axonal transport defects in AD, and our results support the idea that kinesins should be considered as potential drug targets to help reduce dementia-associated disorders.","PeriodicalId":19629,"journal":{"name":"Open Biology","volume":"16 5","pages":""},"PeriodicalIF":3.1,"publicationDate":"2026-05-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147932055","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Macrophages warrant Mauthner cell axon regrowth by preventing late-stage hyperglycemia in zebrafish. 巨噬细胞通过预防斑马鱼晚期高血糖而促进毛特纳细胞轴突再生。

IF 3.6 3区生物学

Open Biology Pub Date : 2026-05-06 DOI: 10.1098/rsob.250156

Jing Bai, Siting Lai, Yubei Huang, Jixiang Liu, Zhujuan Huang, Jin Xu

{"title":"Macrophages warrant Mauthner cell axon regrowth by preventing late-stage hyperglycemia in zebrafish.","authors":"Jing Bai, Siting Lai, Yubei Huang, Jixiang Liu, Zhujuan Huang, Jin Xu","doi":"10.1098/rsob.250156","DOIUrl":"https://doi.org/10.1098/rsob.250156","url":null,"abstract":"Axonal regeneration in the central nervous system is imperative for functional restoration following spinal cord injury (SCI). Myeloid cells are key regulators of axonal regeneration, yet their roles are not fully revealed. SCI perturbs glucose metabolism; however, its precise impact on axonal regeneration remains undefined. Moreover, whether myeloid cells orchestrate glucose metabolic responses to facilitate regeneration is unclear. Here, using the zebrafish Mauthner cell axon transection model, we demonstrate that following SCI, myeloid cell deficiency leads to a late-stage glucose surge, which leads to impaired axonal regeneration. We further identify glucagon signalling as a critical molecular determinant of this metabolic dysregulation and show that targeted mutations in gcga or its receptors (gcgra, gcgrb) rescue the axonal regeneration defects caused by myeloid cell deficiency. Finally, cell-depletion experiments demonstrated that macrophages are responsible for the late-stage hyperglycemia and defective axon regeneration of Mauthner cells. These findings suggest that glucose metabolism plays a critical role in macrophage-warranted axon regeneration in the spinal cord, positioning glucose homeostasis as a potential therapeutic target for enhancing axon regeneration and recovery.","PeriodicalId":19629,"journal":{"name":"Open Biology","volume":"16 5","pages":""},"PeriodicalIF":3.6,"publicationDate":"2026-05-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147841388","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Is the alpha-amylase paralogue Amyrel dispensable in Drosophila melanogaster? 在黑腹果蝇中，α -淀粉酶副淀粉酶是可有可无的吗？

IF 3.1 3区生物学

Open Biology Pub Date : 2026-04-29 DOI: 10.1098/rsob.250411

Jean-Luc Da Lage, Magalie Bonneau, Céline Moreno, Arnaud Le Rouzic

{"title":"Is the alpha-amylase paralogue Amyrel dispensable in Drosophila melanogaster?","authors":"Jean-Luc Da Lage, Magalie Bonneau, Céline Moreno, Arnaud Le Rouzic","doi":"10.1098/rsob.250411","DOIUrl":"10.1098/rsob.250411","url":null,"abstract":"Divergent duplicated gene copies are considered to get new or variant function or regulation through sub- or neofunctionalization. In Drosophila and other flies (Muscomorpha), the alpha-amylase paralogue Amyrel is known to have peculiar enzymological properties compared with the classical enzyme Amy. Yet, its real function in fly biology is unclear. Here, we show that Amyrel and Amy share similar regulation patterns such as sugar downregulation and midgut-specific expression in Drosophila melanogaster. Most regulatory information lies within 500 bp of the upstream sequence, as enhanced green fluorescent protein expression under the Amyrel promoter mimics Amyrel expression quite well. To get an insight into Amyrel function, we knocked out the gene using CRISPR-Cas9. Setting a competition experiment between wild-type (wt) and null alleles over 40 generations, we estimated the selective advantage of the wt to be 2%. However, Amyrel-null mutant lines exhibited no clear defect in several life history traits. Interestingly, while Amyrel had very low expression in young adults, it was significantly upregulated in females aged two months; however, lifespan was not affected. Overall, we were able to document substantial functional and regulatory differences between the Amyrel copy and the regular amylase, and we showed that carrying an Amyrel gene conferred a competitive fitness advantage.","PeriodicalId":19629,"journal":{"name":"Open Biology","volume":"16 4","pages":""},"PeriodicalIF":3.1,"publicationDate":"2026-04-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147777835","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Shaping the microvascular network: insights into skeletal muscle angiogenesis. 塑造微血管网络：对骨骼肌血管生成的洞察。

IF 3.1 3区生物学

Open Biology Pub Date : 2026-04-29 DOI: 10.1098/rsob.250183

Thomas Gustafsson, Emmanuel Nwadozi, Andrea Tryfonos, Tara L Haas

{"title":"Shaping the microvascular network: insights into skeletal muscle angiogenesis.","authors":"Thomas Gustafsson, Emmanuel Nwadozi, Andrea Tryfonos, Tara L Haas","doi":"10.1098/rsob.250183","DOIUrl":"10.1098/rsob.250183","url":null,"abstract":"Angiogenesis, the formation of new blood vessels from pre-existing vasculature, is a complex and tightly regulated biological process that plays a fundamental role in both physiological and pathological tissue remodeling by facilitating the delivery of oxygen and nutrients. Over recent decades, extensive research has identified a wide array of factors that regulate the balance between endothelial cell quiescence and activation. This review discusses the cellular events and molecular mechanisms that regulate angiogenesis within skeletal muscle, considering dynamic interactions with the extracellular matrix and highlighting the critical involvement of multiple resident and infiltrating cell types-including myofibres, satellite cells, fibro-adipogenic progenitors, immune cells and pericytes. The current understanding of these regulatory networks is examined in both healthy muscle tissue as part of the phenotype changes that occur during exercise and in pathological conditions that affect skeletal muscle angiogenesis. Particular attention is given to introduce data of emerging high-resolution techniques, especially omics-based approaches such as single-cell RNA sequencing (scRNA-seq) of skeletal muscle tissue. These methodologies hold significant promise for elucidating cell-type-specific roles and intercellular interactions that drive angiogenic processes in both physiological and disease contexts. Despite substantial progress, the precise mechanisms governing angiogenesis in skeletal muscle remain only partially understood.","PeriodicalId":19629,"journal":{"name":"Open Biology","volume":"16 5","pages":""},"PeriodicalIF":3.1,"publicationDate":"2026-04-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147974469","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Oocyst: knowns and unknowns about the lengthiest life stage of the malaria parasite. 卵囊：已知和未知的疟疾寄生虫最长的生命阶段。

IF 3.1 3区生物学

Open Biology Pub Date : 2026-04-22 DOI: 10.1098/rsob.260009

Alexander J Bailey, Dina Vlachou, George K Christophides

{"title":"Oocyst: knowns and unknowns about the lengthiest life stage of the malaria parasite.","authors":"Alexander J Bailey, Dina Vlachou, George K Christophides","doi":"10.1098/rsob.260009","DOIUrl":"10.1098/rsob.260009","url":null,"abstract":"The oocyst is the longest life stage of Plasmodium, the causative agent of malaria, one of the most persistent and devastating infectious diseases of humankind. Following ingestion during blood feeding, parasites reproduce sexually and traverse the mosquito midgut epithelium to differentiate into oocysts on the basal lamina, where they undergo prolonged development, ultimately giving rise to thousands of sporozoites capable of infecting a new human host. Oocyst formation represents a severe population bottleneck, resulting in the lowest parasite numbers observed across the parasite life cycle. Given its extended duration and pronounced numerical vulnerability, it is striking that the oocyst remains one of the least explored stages of Plasmodium development. Major gaps persist in our understanding of the molecular and cellular processes governing oocyst growth and differentiation, including transcriptional and epigenetic regulation, nutrient acquisition and metabolic remodelling, cell cycle control and interactions with the mosquito immune system and physiology. Recent technological advances and renewed interest in mosquito-stage biology provide an opportunity to dissect these processes at unprecedented resolution. In this review, we synthesize knowledge of oocyst biology, highlight key unresolved questions and discuss how deeper insight into this critical stage could inform the development of next-generation transmission-blocking strategies and accelerate progress towards malaria elimination.","PeriodicalId":19629,"journal":{"name":"Open Biology","volume":"16 4","pages":""},"PeriodicalIF":3.1,"publicationDate":"2026-04-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147777766","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Unveiling karyoskeletal dynamics in chromatin organization and gene regulation in haematopoietic cell physiology and pathology. 揭示染色质组织中的核骨骼动力学和造血细胞生理病理中的基因调控。

IF 3.1 3区生物学

Open Biology Pub Date : 2026-04-22 DOI: 10.1098/rsob.260011

Mohd Kamran, Nicolas Nassar, Jose A Cancelas

{"title":"Unveiling karyoskeletal dynamics in chromatin organization and gene regulation in haematopoietic cell physiology and pathology.","authors":"Mohd Kamran, Nicolas Nassar, Jose A Cancelas","doi":"10.1098/rsob.260011","DOIUrl":"10.1098/rsob.260011","url":null,"abstract":"Chromatin organization and gene regulation are essential for maintaining homeostatic haematopoiesis, the tightly regulated process by which haematopoietic stem and progenitor cells generate diverse blood lineages. Growing evidence identifies karyoskeletal proteins, particularly nuclear actin and its associated scaffolding networks, as central regulators of nuclear function. These proteins influence transcription through interactions with chromatin-remodelling complexes, shape three-dimensional genome architecture, facilitate DNA repair and modulate polycomb-mediated gene repression, thereby governing blood cell identity and lineage commitment. Dysregulation of nuclear structural proteins contributes to haematological malignancies, including leukaemia and myelodysplastic syndromes. In this review, we synthesize current understanding of how karyoskeletal proteins integrate structural and regulatory roles to control chromatin dynamics and gene expression in the lympho-haematopoietic system. We highlight recent insights into their roles in three-dimensional genome organization, epigenetic regulation, polycomb-dependent repression and stemness in both normal and pathological contexts and discuss how nuclear actin modulates these processes in cancer pathogenesis. Collectively, this review underscores how emerging concepts in nuclear architecture reveal karyoskeletal proteins as pivotal determinants of transcriptional regulation and haematopoietic function.","PeriodicalId":19629,"journal":{"name":"Open Biology","volume":"16 4","pages":""},"PeriodicalIF":3.1,"publicationDate":"2026-04-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147777820","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

tRNA modification landscapes in streptococci: shared losses and clade-specific adaptations. 链球菌中的tRNA修饰景观：共同损失和支系特异性适应。

IF 3.1 3区生物学

Open Biology Pub Date : 2026-04-15 DOI: 10.1098/rsob.250445

Ho-Ching Tiffany Tsui, Chi-Kong Chan, Yifeng Yuan, Roba Elias, Jingjing Sun, Virginie Marchand, Marshall Jaroch, Guangxin Sun, Irfan Manzoor, Ana Kutchuashvili, Grazyna Leszczynska, Kinda Seaton, Yuri Motorin, Kelly Rice, Manal Swairjo, Peter C Dedon, Malcolm E Winkler, Valérie de Crécy-Lagard

{"title":"tRNA modification landscapes in streptococci: shared losses and clade-specific adaptations.","authors":"Ho-Ching Tiffany Tsui, Chi-Kong Chan, Yifeng Yuan, Roba Elias, Jingjing Sun, Virginie Marchand, Marshall Jaroch, Guangxin Sun, Irfan Manzoor, Ana Kutchuashvili, Grazyna Leszczynska, Kinda Seaton, Yuri Motorin, Kelly Rice, Manal Swairjo, Peter C Dedon, Malcolm E Winkler, Valérie de Crécy-Lagard","doi":"10.1098/rsob.250445","DOIUrl":"10.1098/rsob.250445","url":null,"abstract":"tRNA modifications are central to bacterial translational control. Here, we integrated genetics, mass spectrometry, epitranscriptomics and comparative genomics to map the tRNA modification genes of the Gram-positive pathogens Streptococcus mutans and Streptococcus pneumoniae. Both species show a marked loss of modifications dependent on Fe-S enzymes, consistent with a broader trend of Fe-S enzyme reduction in Streptococcus central metabolism. In addition, the D, m1A, m7G, t6A and i6A modifications were mapped in S. pneumoniae tRNAs, and we confirmed that a unique DusB1 enzyme is responsible for the insertion of all the detectable D modifications. We uncovered differences in queuosine (Q) metabolism: while S. mutans synthesizes Q de novo, S. pneumoniae instead salvages preQ₁ and accumulates the epoxy-Q precursor, a strategy shared with multiple other streptococci as revealed by analysis of Q pathways in 1599 sequenced streptococcal genomes. Comparative essentiality profiling of modification genes revealed notable differences, including the essentiality of the N⁶-threonylcarbamoyladenosine (t⁶A) synthesis enzyme TsaE in S. pneumoniae but not in S. mutans, which was confirmed by genetic studies. We found that suppressor mutations in asnS encoding asparaginyl-tRNA synthetase (AsnRS) restored viability to ∆tsaE mutants, albeit with reduced growth. Our finding highlights the functional importance of modifications in the recognition of tRNAs by aminoacyl-tRNA synthetases.","PeriodicalId":19629,"journal":{"name":"Open Biology","volume":"16 4","pages":""},"PeriodicalIF":3.1,"publicationDate":"2026-04-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147691442","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Mechanical bone loading effects on morphology and mechanobiology in the coronal suture of Crouzon mice. 机械骨负荷对Crouzon小鼠冠状缝合形态和力学生物学的影响。

IF 3.1 3区生物学

Open Biology Pub Date : 2026-04-15 DOI: 10.1098/rsob.250387

Miranda Steacy, Marius Didziokas, Tengyang Qiu, Damith S Chathuranga, Ce Liang, Ali Alazmani, Dale Moulding, Oliver Gardner, Mehran Moazen, Erwin Pauws

{"title":"Mechanical bone loading effects on morphology and mechanobiology in the coronal suture of Crouzon mice.","authors":"Miranda Steacy, Marius Didziokas, Tengyang Qiu, Damith S Chathuranga, Ce Liang, Ali Alazmani, Dale Moulding, Oliver Gardner, Mehran Moazen, Erwin Pauws","doi":"10.1098/rsob.250387","DOIUrl":"10.1098/rsob.250387","url":null,"abstract":"Craniosynostosis is a congenital condition characterized by the premature fusion of the craniofacial sutures. The Crouzon mouse (Fgfr2cC342Y/+) is a well-established model of this condition which shows premature fusion of the coronal suture. Our group has recently shown that postnatal, cyclic loading can potentially rescue the coronal suture and normalize skull morphology in Crouzon mice. This study aimed to investigate the underlying biological mechanism of the treatment. Wild-type (WT) and Crouzon (MUT) mice underwent in vivo loading sessions. Loading did not significantly affect skull shape. The patency across the coronal suture did not change between treated and untreated MUT animals. Orientation and coherence of the coronal suture collagen fibres were statistically different when comparing WT untreated with MUT untreated and WT treated with MUT treated. Treatment increases the number of proliferative cells in both the WT and MUT sutures compared to their untreated counterparts. The mechanobiological mechanisms driving the differences need further investigation into molecular mechanotransduction pathways. Understanding the biological principles affected during bone loading, a more refined cyclical bone loading protocol can be developed and refined for potential clinical use.","PeriodicalId":19629,"journal":{"name":"Open Biology","volume":"16 4","pages":""},"PeriodicalIF":3.1,"publicationDate":"2026-04-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147691451","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0