Open Biology最新文献_第4页

High-throughput functional analysis provides novel insight into type VII secretion in Staphylococcus aureus. 高通量功能分析为金黄色葡萄球菌的 VII 型分泌提供了新的视角。

IF 4.5 3区生物学

Open Biology Pub Date : 2024-08-01 Epub Date: 2024-08-14 DOI: 10.1098/rsob.240060

Yaping Yang, Aaron A Scott, Holger Kneuper, Felicity Alcock, Tracy Palmer

{"title":"High-throughput functional analysis provides novel insight into type VII secretion in Staphylococcus aureus.","authors":"Yaping Yang, Aaron A Scott, Holger Kneuper, Felicity Alcock, Tracy Palmer","doi":"10.1098/rsob.240060","DOIUrl":"10.1098/rsob.240060","url":null,"abstract":"Successful colonization by the opportunistic pathogen Staphylococcus aureus depends on its ability to interact with other microorganisms. Staphylococcus aureus strains harbour a T7b subtype of type VII secretion system (T7SSb), a protein secretion system found in a wide variety of Bacillota, which functions in bacterial antagonism and virulence. Assessment of T7SSb activity in S. aureus has been hampered by low secretion activity under laboratory conditions and the lack of a sensitive assay to measure secretion. Here, we have utilized NanoLuc binary technology to develop a simple assay to monitor protein secretion via detection of bioluminescence. Fusion of the 11 amino acid NanoLuc fragment to the conserved substrate EsxA permits its extracellular detection upon supplementation with the large NanoLuc fragment and luciferase substrate. Following miniaturization of the assay to 384-well format, we use high-throughput analysis to demonstrate that T7SSb-dependent protein secretion differs across strains and growth temperature. We further show that the same assay can be used to monitor secretion of the surface-associated toxin substrate TspA. Using this approach, we identify three conserved accessory proteins required to mediate TspA secretion. Co-purification experiments confirm that all three proteins form a complex with TspA.","PeriodicalId":19629,"journal":{"name":"Open Biology","volume":"14 8","pages":"240060"},"PeriodicalIF":4.5,"publicationDate":"2024-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11322744/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141976295","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A feather star is born: embryonic development and nervous system organization in the crinoid Antedon mediterranea. 羽毛之星的诞生：地中海蚁蜥的胚胎发育和神经系统组织。

IF 4.5 3区生物学

Open Biology Pub Date : 2024-08-01 Epub Date: 2024-08-21 DOI: 10.1098/rsob.240115

Silvia Mercurio, Giacomo Gattoni, Giorgio Scarì, Miriam Ascagni, Benedetta Barzaghi, Maurice R Elphick, Jenifer C Croce, Michael Schubert, Elia Benito-Gutiérrez, Roberta Pennati

{"title":"A feather star is born: embryonic development and nervous system organization in the crinoid Antedon mediterranea.","authors":"Silvia Mercurio, Giacomo Gattoni, Giorgio Scarì, Miriam Ascagni, Benedetta Barzaghi, Maurice R Elphick, Jenifer C Croce, Michael Schubert, Elia Benito-Gutiérrez, Roberta Pennati","doi":"10.1098/rsob.240115","DOIUrl":"10.1098/rsob.240115","url":null,"abstract":"Crinoids belong to the Echinodermata, marine invertebrates with a highly derived adult pentaradial body plan. As the sister group to all other extant echinoderms, crinoids occupy a key phylogenetic position to explore the evolutionary history of the whole phylum. However, their development remains understudied compared with that of other echinoderms. Therefore, the aim here was to establish the Mediterranean feather star (Antedon mediterranea) as an experimental system for developmental biology. We first set up a method for culturing embryos in vitro and defined a standardized staging system for this species. We then optimized protocols to characterize the morphological and molecular development of the main structures of the feather star body plan. Focusing on the nervous system, we showed that the larval apical organ includes serotonergic, GABAergic and glutamatergic neurons, which develop within a conserved anterior molecular signature. We described the composition of the early post-metamorphic nervous system and revealed that it has an anterior signature. These results further our knowledge on crinoid development and provide new techniques to investigate feather star embryogenesis. This will pave the way for the inclusion of crinoids in comparative studies addressing the origin of the echinoderm body plan and the evolutionary diversification of deuterostomes.","PeriodicalId":19629,"journal":{"name":"Open Biology","volume":"14 8","pages":"240115"},"PeriodicalIF":4.5,"publicationDate":"2024-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11336682/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142009177","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Molecular basis of urostyle development in frogs: genes and gene regulation underlying an evolutionary novelty. 蛙类尿道发育的分子基础：一种进化新现象背后的基因和基因调控。

IF 4.5 3区生物学

Open Biology Pub Date : 2024-08-01 Epub Date: 2024-08-28 DOI: 10.1098/rsob.240111

Gayani Senevirathne, Neil H Shubin

{"title":"Molecular basis of urostyle development in frogs: genes and gene regulation underlying an evolutionary novelty.","authors":"Gayani Senevirathne, Neil H Shubin","doi":"10.1098/rsob.240111","DOIUrl":"10.1098/rsob.240111","url":null,"abstract":"Evolutionary novelties entail the origin of morphologies that enable new functions. These features can arise through changes to gene function and regulation. One key novelty is the fused rod at the end of the vertebral column in anurans, the urostyle. This feature is composed of a coccyx and a hypochord, both of which ossify during metamorphosis. To elucidate the genetic basis of these features, we used laser capture microdissection of these tissues and did RNA-seq and ATAC-seq at three developmental stages in tadpoles of Xenopus tropicalis. RNA-seq reveals that the coccyx and hypochord have two different molecular signatures. Neuronal (TUBB3) and muscle markers (MYH3) are upregulated in coccygeal tissues, whereas T-box genes (TBXT, TBXT.2), corticosteroid stress hormones (CRCH.1) and matrix metallopeptidases (MMP1, MMP8 and MMP13) are upregulated in the hypochord. ATAC-seq reveals potential regulatory regions that are observed in proximity to candidate genes that regulate ossification identified from RNA-seq. Even though an ossifying hypochord is only present in anurans, this ossification between the vertebral column and the notochord resembles a congenital vertebral anomaly seen prenatally in humans caused by an ectopic expression of the TBXT/TBXT.2 gene. This work opens the way to functional studies that can elucidate anuran bauplan evolution.","PeriodicalId":19629,"journal":{"name":"Open Biology","volume":"14 8","pages":"240111"},"PeriodicalIF":4.5,"publicationDate":"2024-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11349433/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142081206","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Exploring the midgut physiology of the non-haematophagous mosquito Toxorhynchites theobaldi. 探索非食血蚊Toxorhynchites theobaldi的中肠生理学。

IF 4.5 3区生物学

Open Biology Pub Date : 2024-07-01 Epub Date: 2024-07-03 DOI: 10.1098/rsob.230437

Renata C Barbosa, Raquel S M Godoy, Priscila G Ferreira, Tiago A O Mendes, Marcelo Ramalho-Ortigão, José M C Ribeiro, Gustavo F Martins

{"title":"Exploring the midgut physiology of the non-haematophagous mosquito Toxorhynchites theobaldi.","authors":"Renata C Barbosa, Raquel S M Godoy, Priscila G Ferreira, Tiago A O Mendes, Marcelo Ramalho-Ortigão, José M C Ribeiro, Gustavo F Martins","doi":"10.1098/rsob.230437","DOIUrl":"10.1098/rsob.230437","url":null,"abstract":"Toxorhynchites mosquitoes have an exclusively phytophagous feeding habit as adults, which leads to significant differences in their morphophysiology compared with haematophagous mosquitoes. However, the molecular mechanisms of digestion in this mosquito are not well understood. In this study, RNA sequencing of the posterior midgut (PMG) of the mosquito Toxorhynchites theobaldi was undertaken, highlighting its significance in mosquito digestion. Subsequently, a comparison was made between the differential gene expression of the PMG and that of the anterior midgut. It was found that the most abundant proteases in the PMG were trypsin and chymotrypsin, and the level of gene expression for enzymes essential for digestion (such as serine protease, α-amylase and pancreatic triacylglycerol lipase) and innate immune response (including catalase, cecropin-A2 and superoxide dismutase) was like that of haematophagous mosquitoes. Peritrophin-1 was detected in the entire midgut, with an elevated expression level in the PMG. Based on our findings, it is hypothesized that a non-haematophagic habit might have been exhibited by the ancestor of Tx. theobaldi, and this trait may have been retained. This study represents a pioneering investigation at the molecular level of midgut contents in a non-haematophagous mosquito. The findings offer valuable insights into the evolutionary aspects of feeding habits in culicids.","PeriodicalId":19629,"journal":{"name":"Open Biology","volume":"14 7","pages":"230437"},"PeriodicalIF":4.5,"publicationDate":"2024-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11286187/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141492872","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Glutamate transporters are involved in direct inhibitory synaptic transmission in the vertebrate retina. 谷氨酸转运体参与脊椎动物视网膜的直接抑制性突触传递。

IF 4.5 3区生物学

Open Biology Pub Date : 2024-07-01 Epub Date: 2024-07-31 DOI: 10.1098/rsob.240140

Stephanie Niklaus, Stella M K Glasauer, Peter Kovermann, Kulsum F Farshori, Lucia Cadetti, Simon Früh, Nicolas N Rieser, Matthias Gesemann, Jingjing Zang, Christoph Fahlke, Stephan C F Neuhauss

{"title":"Glutamate transporters are involved in direct inhibitory synaptic transmission in the vertebrate retina.","authors":"Stephanie Niklaus, Stella M K Glasauer, Peter Kovermann, Kulsum F Farshori, Lucia Cadetti, Simon Früh, Nicolas N Rieser, Matthias Gesemann, Jingjing Zang, Christoph Fahlke, Stephan C F Neuhauss","doi":"10.1098/rsob.240140","DOIUrl":"10.1098/rsob.240140","url":null,"abstract":"In the central nervous system of vertebrates, glutamate serves as the primary excitatory neurotransmitter. However, in the retina, glutamate released from photoreceptors causes hyperpolarization in post-synaptic ON-bipolar cells through a glutamate-gated chloride current, which seems paradoxical. Our research reveals that this current is modulated by two excitatory glutamate transporters, EAAT5b and EAAT7. In the zebrafish retina, these transporters are located at the dendritic tips of ON-bipolar cells and interact with all four types of cone photoreceptors. The absence of these transporters leads to a decrease in ON-bipolar cell responses, with eaat5b mutants being less severely affected than eaat5b/eaat7 double mutants, which also exhibit altered response kinetics. Biophysical investigations establish that EAAT7 is an active glutamate transporter with a predominant anion conductance. Our study is the first to demonstrate the direct involvement of post-synaptic glutamate transporters in inhibitory direct synaptic transmission at a central nervous system synapse.","PeriodicalId":19629,"journal":{"name":"Open Biology","volume":"14 7","pages":"240140"},"PeriodicalIF":4.5,"publicationDate":"2024-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11288666/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141856143","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Human EWS-FLI protein levels and neomorphic functions show a complex, function-specific dose-response relationship in Drosophila. 果蝇体内人类 EWS-FLI 蛋白水平和新形态功能显示出复杂的、功能特异性剂量反应关系。

IF 4.5 3区生物学

Open Biology Pub Date : 2024-07-01 Epub Date: 2024-07-17 DOI: 10.1098/rsob.240043

Serena Mahnoor, Cristina Molnar, Diego Velázquez, Jose Reina, Salud Llamazares, Jan Peter Heinen, Jaume Mora, Cayetano Gonzalez

{"title":"Human EWS-FLI protein levels and neomorphic functions show a complex, function-specific dose-response relationship in Drosophila.","authors":"Serena Mahnoor, Cristina Molnar, Diego Velázquez, Jose Reina, Salud Llamazares, Jan Peter Heinen, Jaume Mora, Cayetano Gonzalez","doi":"10.1098/rsob.240043","DOIUrl":"10.1098/rsob.240043","url":null,"abstract":"Ewing sarcoma (EwS) is a cancer that arises in the bones and soft tissues, typically driven by the Ewing's sarcoma breakpoint region 1-Friend leukemia virus integration 1 (EWS-FLI) oncogene. Implementation of genetically modified animal models of EwS has proved difficult largely owing to EWS-FLI's high toxicity. The EWS-FLI1FS frameshift variant that circumvents toxicity but is still able to perform key oncogenic functions provided the first study model in Drosophila. However, the quest for Drosophila lines expressing full-length, unmodified EWS-FLI remained open. Here, we show that EWS-FLI1FS's lower toxicity is owed to reduced protein levels caused by its frameshifted C-terminal peptide, and report new strategies through which we have generated Drosophila lines that express full-length, unmodified EWS-FLI. Using these lines, we have found that the upregulation of transcription from GGAA-microsatellites (GGAAμSats) presents a positive linear correlation within a wide range of EWS-FLI protein concentrations. In contrast, rather counterintuitively, GGAAμSats-independent transcriptomic dysregulation presents relatively minor differences across the same range, suggesting that GGAAμSat-dependent and -independent transcriptional upregulation present different kinetics of response with regards to changing EWS-FLI protein concentration. Our results underpin the functional relevance of varying EWS-FLI expression levels and provide experimental tools to investigate, in Drosophila, the effect of the EWS-FLI 'high' and 'low' states that have been reported and are suspected to be important for EwS in humans.","PeriodicalId":19629,"journal":{"name":"Open Biology","volume":"14 7","pages":"240043"},"PeriodicalIF":4.5,"publicationDate":"2024-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11251760/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141627242","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The CoREST complex regulates multiple histone modifications temporal-specifically in clock neurons. CoREST复合体在时钟神经元中调节多种组蛋白修饰的时间特异性。

IF 4.5 3区生物学

Open Biology Pub Date : 2024-07-01 Epub Date: 2024-07-10 DOI: 10.1098/rsob.230355

Pengfei Lv, Zhangwu Zhao, Yukinori Hirano, Juan Du

{"title":"The CoREST complex regulates multiple histone modifications temporal-specifically in clock neurons.","authors":"Pengfei Lv, Zhangwu Zhao, Yukinori Hirano, Juan Du","doi":"10.1098/rsob.230355","DOIUrl":"10.1098/rsob.230355","url":null,"abstract":"Epigenetic regulation is important for circadian rhythm. In previous studies, multiple histone modifications were found at the Period (Per) locus. However, most of these studies were not conducted in clock neurons. In our screen, we found that a CoREST mutation resulted in defects in circadian rhythm by affecting Per transcription. Based on previous studies, we hypothesized that CoREST regulates circadian rhythm by regulating multiple histone modifiers at the Per locus. Genetic and physical interaction experiments supported these regulatory relationships. Moreover, through tissue-specific chromatin immunoprecipitation assays in clock neurons, we found that the CoREST mutation led to time-dependent changes in corresponding histone modifications at the Per locus. Finally, we proposed a model indicating the role of the CoREST complex in the regulation of circadian rhythm. This study revealed the dynamic changes of histone modifications at the Per locus specifically in clock neurons. Importantly, it provides insights into the role of epigenetic factors in the regulation of dynamic gene expression changes in circadian rhythm.","PeriodicalId":19629,"journal":{"name":"Open Biology","volume":"14 7","pages":"230355"},"PeriodicalIF":4.5,"publicationDate":"2024-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11285899/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141564011","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Unravelling nicotinic receptor and ligand features underlying neonicotinoid knockdown actions on the malaria vector mosquito Anopheles gambiae. 揭示新烟碱类药物对疟疾病媒冈比亚按蚊的抑制作用所依赖的烟碱受体和配体特征。

IF 4.5 3区生物学

Open Biology Pub Date : 2024-07-01 Epub Date: 2024-07-24 DOI: 10.1098/rsob.240057

Ryo Ito, Masaki Kamiya, Koichi Takayama, Sumito Mori, Rei Matsumoto, Mayuka Takebayashi, Hisanori Ojima, Shota Fujimura, Haruki Yamamoto, Masayuki Ohno, Makoto Ihara, Toshihide Okajima, Atsuko Yamashita, Fraser Colman, Gareth J Lycett, David B Sattelle, Kazuhiko Matsuda

{"title":"Unravelling nicotinic receptor and ligand features underlying neonicotinoid knockdown actions on the malaria vector mosquito Anopheles gambiae.","authors":"Ryo Ito, Masaki Kamiya, Koichi Takayama, Sumito Mori, Rei Matsumoto, Mayuka Takebayashi, Hisanori Ojima, Shota Fujimura, Haruki Yamamoto, Masayuki Ohno, Makoto Ihara, Toshihide Okajima, Atsuko Yamashita, Fraser Colman, Gareth J Lycett, David B Sattelle, Kazuhiko Matsuda","doi":"10.1098/rsob.240057","DOIUrl":"10.1098/rsob.240057","url":null,"abstract":"With the spread of resistance to long-established insecticides targeting Anopheles malaria vectors, understanding the actions of compounds newly identified for vector control is essential. With new commercial vector-control products containing neonicotinoids under development, we investigate the actions of 6 neonicotinoids (imidacloprid, thiacloprid, clothianidin, dinotefuran, nitenpyram and acetamiprid) on 13 Anopheles gambiae nicotinic acetylcholine receptor (nAChR) subtypes produced by expression of combinations of the Agα1, Agα2, Agα3, Agα8 and Agβ1 subunits in Xenopus laevis oocytes, the Drosophila melanogaster orthologues of which we have previously shown to be important in neonicotinoid actions. The presence of the Agα2 subunit reduces neonicotinoid affinity for the mosquito nAChRs, whereas the Agα3 subunit increases it. Crystal structures of the acetylcholine binding protein (AChBP), an established surrogate for the ligand-binding domain, with dinotefuran bound, shows a unique target site interaction through hydrogen bond formation and CH-N interaction at the tetrahydrofuran ring. This is of interest as dinotefuran is also under trial as the toxic element in baited traps. Multiple regression analyses show a correlation between the efficacy of neonicotinoids for the Agα1/Agα2/Agα8/Agβ1 nAChR, their hydrophobicity and their rate of knockdown of adult female An. gambiae, providing new insights into neonicotinoid features important for malaria vector control.","PeriodicalId":19629,"journal":{"name":"Open Biology","volume":"14 7","pages":"240057"},"PeriodicalIF":4.5,"publicationDate":"2024-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11265914/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141752300","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Pseudogenization of NK3 homeobox 2 (Nkx3.2) in monotremes provides insight into unique gastric anatomy and physiology. 单孔类动物NK3同源染色体2（Nkx3.2）的假基因化为独特的胃解剖学和生理学提供了启示。

IF 4.5 3区生物学

Open Biology Pub Date : 2024-07-01 Epub Date: 2024-07-03 DOI: 10.1098/rsob.240071

Jackson Dann, Zhipeng Qu, Linda Shearwin-Whyatt, Rachel van der Ploeg, Frank Grützner

{"title":"Pseudogenization of NK3 homeobox 2 (Nkx3.2) in monotremes provides insight into unique gastric anatomy and physiology.","authors":"Jackson Dann, Zhipeng Qu, Linda Shearwin-Whyatt, Rachel van der Ploeg, Frank Grützner","doi":"10.1098/rsob.240071","DOIUrl":"10.1098/rsob.240071","url":null,"abstract":"The enzymatic breakdown and regulation of food passage through the vertebrate antral stomach and pyloric sphincter (antropyloric region) is a trait conserved over 450 million years. Development of the structures involved is underpinned by a highly conserved signalling pathway involving the hedgehog, bone morphogenetic protein and Wingless/Int-1 (Wnt) protein families. Monotremes are one of the few vertebrate lineages where acid-based digestion has been lost, and this is consistent with the lack of genes for hydrochloric acid secretion and gastric enzymes in the genomes of the platypus (Ornithorhynchus anatinus) and short-beaked echidna (Tachyglossus aculeatus) . Furthermore, these species feature unique gastric phenotypes, both with truncated and aglandular antral stomachs and the platypus with no pylorus. Here, we explore the genetic underpinning of monotreme gastric phenotypes, investigating genes important in antropyloric development using the newest monotreme genomes (mOrnAna1.pri.v4 and mTacAcu1) together with RNA-seq data. We found that the pathway constituents are generally conserved, but surprisingly, NK3 homeobox 2 (Nkx3.2) was pseudogenized in both platypus and echidna. We speculate that the unique sequence evolution of Grem1 and Bmp4 sequences in the echidna lineage may correlate with their pyloric-like restriction and that the convergent loss of gastric acid and stomach size genotypes and phenotypes in teleost and monotreme lineages may be a result of eco-evolutionary dynamics. These findings reflect the effects of gene loss on phenotypic evolution and further elucidate the genetic control of monotreme stomach anatomy and physiology.","PeriodicalId":19629,"journal":{"name":"Open Biology","volume":"14 7","pages":"240071"},"PeriodicalIF":4.5,"publicationDate":"2024-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11285752/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141492922","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Recent progress in dendritic pruning of Drosophila C4da sensory neurons. 果蝇 C4da 感觉神经元树突修剪的最新进展

IF 4.5 3区生物学

Open Biology Pub Date : 2024-07-01 Epub Date: 2024-07-24 DOI: 10.1098/rsob.240059

Menglong Rui

引用次数: 0