One Health最新文献

{"title":"Prevalence, antibiotic resistance, resistance and virulence determinants of Campylobacter jejuni in China: A systematic review and meta-analysis","authors":"Zhao Li ,&nbsp;Hua Cai ,&nbsp;Biyao Xu ,&nbsp;Qingli Dong ,&nbsp;Kai Jia ,&nbsp;Zijie Lin ,&nbsp;Xiang Wang ,&nbsp;Yangtai Liu ,&nbsp;Xiaojie Qin","doi":"10.1016/j.onehlt.2025.100990","DOIUrl":"10.1016/j.onehlt.2025.100990","url":null,"abstract":"<div><div><em>Campylobacter jejuni</em> (<em>C. jejuni</em>) is recognized as a serious food contaminant that extensively results in foodborne diseases. Numerous studies have been conducted on the prevalence and antibiotic resistance of <em>C. jejuni</em>, but there is a lack of comprehensive analysis of published data. This study provides a comprehensive overview of the epidemiology, antibiotic resistance, and virulence determinants of <em>C. jejuni</em> in China through a systematic review and meta-analysis. The prevalence levels of <em>C. jejuni</em> from low to high were the humans (5.2 %, 95 % CI: 4.2–6.4 %), foods (12.5 %, 95 % CI: 9.7–15.6 %), animals (15.4 %, 95 % CI: 13.2–17.6 %), and environment (17.8 %, 95 % CI: 9.7–27.7 %), respectively. Furthermore, <em>C. jejuni</em> exhibits high resistance rates to antibiotics such as cefoperazone, nalidixic acid, ciprofloxacin, cefradine, and tetracycline. The overall multi-drug resistance rate (MDR) of <em>C. jejuni</em> was 72.8 % (95 % CI: 62.4–82.2 %), indicating a serious problem with MDR. The resistance of <em>C. jejuni</em> to most antibiotics has increased in the last 20 years. Among the main resistance determinants of <em>C. jejuni</em>, <em>gyrA</em>_T86I and <em>tet</em>(O) had a higher pooled prevalence of 94.8 % (95 % CI: 88.7–99.0 %) and 79.0 % (95 % CI: 66.9–89.2 %), respectively. Furthermore, the high prevalence of virulence-related genes was shown in <em>C. jejuni</em>, such as adhesion (<em>cadF</em>, <em>racR</em>), invasion (<em>ciaB</em>, <em>iamA</em>, <em>ceuE</em>), and toxin (<em>cdtB, cdtC</em>). In summary, <em>C. jejuni</em> has a high prevalence with regional characteristics, and antibiotic resistance of this bacterium especially animal sources remains a serious problem in China. Comprehensive monitoring and control measures for this pathogen are urgently needed to ensure food safety and public health.</div></div>","PeriodicalId":19577,"journal":{"name":"One Health","volume":"20 ","pages":"Article 100990"},"PeriodicalIF":4.1,"publicationDate":"2025-02-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143395533","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Potentially pathogenic free-living amoebae at very high altitude: Detection by multiplex qPCR in the Northern Altiplano fascioliasis hyperendemic area in Bolivia","authors":"Patricia Pérez-Pérez ,&nbsp;Patricio Artigas ,&nbsp;María Reyes-Batlle ,&nbsp;Elizabeth Córdoba-Lanús ,&nbsp;Rubén L. Rodríguez-Expósito ,&nbsp;Pablo F. Cuervo ,&nbsp;Angélica Domínguez-de-Barros ,&nbsp;Omar García-Pérez ,&nbsp;M. Adela Valero ,&nbsp;Alejandra De Elías ,&nbsp;René Anglés ,&nbsp;Santiago Mas-Coma ,&nbsp;José E. Piñero ,&nbsp;M. Dolores Bargues ,&nbsp;Jacob Lorenzo-Morales","doi":"10.1016/j.onehlt.2025.100985","DOIUrl":"10.1016/j.onehlt.2025.100985","url":null,"abstract":"<div><div>Free-living amoebae (FLA), which are frequently found in the environment, include opportunistic pathogenic genera/species such as <em>Acanthamoeba</em> spp., <em>Balamuthia mandrillaris</em>, <em>Naegleria fowleri</em> and <em>Vermamoeba vermiformis</em>. These pathogenic FLA are causative agents of amoebic encephalitis and keratitis in the case of <em>Acanthamoeba</em> genus and <em>V. vermiformis</em>. In addition, amoebic infections are often related to contamination of domestic and recreational water sources. This study aimed to identify potentially pathogenic FLA in the hyperendemic area of human fascioliasis in a very-high-altitude area (3800–4100 m a.s.l.) of Bolivia and examine whether an association between both pathogens could be established from the environmental point of view. A total of 55 samples (28 soil and 27 water samples) were collected from various locations in the Northern Altiplano of Bolivia. Samples were processed by multiplex qPCR to detect the four pathogenic FLA genera/species. All samples were positive for the presence of <em>V. vermiformis</em>, followed by <em>Acanthamoeba</em> spp. which was positive in 18 soil and 10 water samples. In contrast, <em>B. mandrillaris</em> was only detected in soil sources, whereas <em>N. fowleri</em> was not detected in any of the samples. The coexistence and diverse distribution of multiple FLA species in many locations at such a high altitude is worth mentioning and indicates a potential risk of coinfections. These findings suggest that FLA surveillance is a crucial factor to be considered when implementing preventive measures and improving public health in fascioliasis hyperendemic areas.</div></div>","PeriodicalId":19577,"journal":{"name":"One Health","volume":"20 ","pages":"Article 100985"},"PeriodicalIF":4.1,"publicationDate":"2025-02-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143403322","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Analysis of antibiotic use and access to drugs among poultry farmers in Kenya","authors":"Dishon M. Muloi ,&nbsp;Mitchelle R. Kasudi ,&nbsp;Maurice K. Murungi ,&nbsp;Eugine Lusanji Ibayi ,&nbsp;Samuel Kahariri ,&nbsp;Charity Karimi ,&nbsp;Max Korir ,&nbsp;Bridgit Muasa ,&nbsp;Damaris Mwololo ,&nbsp;Romona Ndanyi ,&nbsp;Robert Ndungi ,&nbsp;Jane Njiru ,&nbsp;Ruth Omani ,&nbsp;Rose Owada ,&nbsp;Sylvia Omulo ,&nbsp;Allan Azegele ,&nbsp;Eric M. Fèvre","doi":"10.1016/j.onehlt.2025.100987","DOIUrl":"10.1016/j.onehlt.2025.100987","url":null,"abstract":"<div><div>Understanding access to and use of antibiotics in livestock production systems is critical for guiding antimicrobial stewardship programmes and animal health services. We analysed antibiotic use practices among smallholder-intensive poultry farms in Kenya and characterised access to veterinary supply chains by calculating travel time to drug stores.</div><div>Data were collected from 766 poultry farms across 15 Kenyan counties, representing all production types, between May 2021 and February 2022. We also collected antibiotic sales and geolocation data from 321 veterinary drug stores in Nakuru and Kilifi counties, representing areas with high and low-intensity poultry production, respectively. Using a machine learning framework, we predicted farm-level antibiotic use based on collected demographic and production traits. We also built geospatial models to characterise farmer travel time to drug stores with motorised transport.</div><div>Half of farms used antibiotics at least once in the last two months, mostly for self-administered therapeutic reasons. Random forest analysis predicted that farms using disinfectants in cleaning, keeping other poultry species, with rodents in the chicken house and vaccinating their birds had the highest likelihood of antibiotic use. 95.4 % of farmers lived within one hour of a veterinary drug store, with 40 % residing within 15 min.</div><div>Antibiotic use is integrated in smallholder poultry production, emphasising the need for prioritizing biosecurity, regulatory and socio-behavioural interventions, and economic incentives to enhance stewardship. Spatial maps suggests both risks and opportunities for antibiotic access and veterinary care.</div></div>","PeriodicalId":19577,"journal":{"name":"One Health","volume":"20 ","pages":"Article 100987"},"PeriodicalIF":4.1,"publicationDate":"2025-02-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143387305","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of chikungunya, Mayaro and Una virus coinfection on vector competence of Aedes aegypti mosquitoes","authors":"Tessa M. Visser ,&nbsp;Haidong D. Wang ,&nbsp;Sandra R. Abbo ,&nbsp;Chantal B.F. Vogels ,&nbsp;Constantianus J.M. Koenraadt ,&nbsp;Gorben P. Pijlman","doi":"10.1016/j.onehlt.2025.100991","DOIUrl":"10.1016/j.onehlt.2025.100991","url":null,"abstract":"<div><div>The mosquito-borne alphaviruses chikungunya (CHIKV), Mayaro (MAYV) and the lesser known Una (UNAV) are currently co-circulating in Latin America, sharing their geographical and ecological niche with the <em>Aedes aegypti</em> mosquito. The sylvatic MAYV cycle and the unknown cycle of UNAV could possibly spill over and become urban transmission cycles involving <em>Ae. aegypti</em>. Despite their potential impact on public health, we know little about arboviral coinfections in humans, animals or mosquitoes. Especially the effect of coinfections on transmission by <em>Ae. aegypti</em> mosquitoes is understudied. We investigated the vector competence of <em>Ae. aegypti</em> for single, dual, and triple exposures with UNAV, MAYV and CHIKV, provided simultaneously in an infectious blood meal. Mosquitoes were incubated for ten days at 28 °C and 70 % humidity. After RNA extractions from mosquito bodies and saliva, the presence and relative quantity of each virus in coinfected mosquitoes was determined. We show that <em>Ae. aegypti</em> can become infected with all three viruses simultaneously, and transmit at least two alphaviruses in a single mosquito bite after dual or triple infection. Additionally, we show for the first time that <em>Ae. aegypti</em> is a competent vector for UNAV, and that dual infections do not influence vector competence. In triple coinfections, however, the total viral load carried by mosquitoes decreases, lowering the transmission potential. Understanding how coinfections affect arbovirus biology and transmission of is essential for assessing public health risks. However, emerging <em>Ae. aegypti</em>-vectored arboviruses and coinfections are a One Health concern, as ecological and environmental changes will increasingly drive the geographic distributions of viruses, vectors, and hosts in the future.</div></div>","PeriodicalId":19577,"journal":{"name":"One Health","volume":"20 ","pages":"Article 100991"},"PeriodicalIF":4.1,"publicationDate":"2025-02-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143377248","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Disinfectants and one health review: The role of reactive oxygen species in the bactericidal activity of chlorine against Salmonella","authors":"Mohammed Aljuwayd ,&nbsp;Israa Abdullah Malli ,&nbsp;Elena G. Olson ,&nbsp;Steven C. Ricke ,&nbsp;Michael J. Rothrock Jr. ,&nbsp;Young Min Kwon","doi":"10.1016/j.onehlt.2025.100989","DOIUrl":"10.1016/j.onehlt.2025.100989","url":null,"abstract":"<div><div><em>Salmonella</em> are among the most common foodborne pathogens in humans, and they are associated with mild to severe diseases commonly referred to as salmonellosis. The genus resides in various animals' intestinal tracts, including humans. It is one of the most diverse genera of bacteria, including over 2500 serovars. Consumption of poultry products contaminated with <em>Salmonella</em> is a significant source of disease transmission in humans. Because of this food safety concern, the poultry industry and governments spend billions of dollars on <em>Salmonella</em> containment methods. However, a completely effective strategy is yet to be established. Chlorine has been commonly used as a disinfectant in the poultry industry. In humans, antibiotic therapy is the primary means for managing <em>Salmonella</em> infection. However, widespread use of both compounds at sub-inhibitory concentrations has allowed resistant strains to emerge and rapidly spread globally. Both antimicrobial compounds involve generating reactive oxygen species (ROS) as a bactericidal mechanism of action. However, ROS generation and its association with bacterial survival and growth inhibition have not been widely explored. Thus, a better understanding of ROS generation during antimicrobial treatments may help devise better <em>Salmonella</em> containment strategies.</div></div>","PeriodicalId":19577,"journal":{"name":"One Health","volume":"20 ","pages":"Article 100989"},"PeriodicalIF":4.1,"publicationDate":"2025-02-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143403323","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Disease outbreak in wildlife changes online sales of management items","authors":"Tomohiko Endo ,&nbsp;Shinya Uryu ,&nbsp;Keita Fukasawa ,&nbsp;Jiefeng Kang ,&nbsp;Takahiro Kubo","doi":"10.1016/j.onehlt.2025.100988","DOIUrl":"10.1016/j.onehlt.2025.100988","url":null,"abstract":"<div><div>Infectious diseases of wildlife cause human health hazards and economic losses. However, it is unclear how outbreaks affect human behaviour in relation to countermeasures against human–wildlife conflict. To explore the effects of infectious disease outbreaks among wild boars on countermeasure choices, we analysed online auction data before and after an outbreak of classical swine fever in wild boar. Online sales of boar traps decreased by 17 % after the outbreak, whereas sales of control items increased by 73 %. These results imply that infectious disease outbreaks in wildlife shift people's countermeasures from active to passive management. Since active trapping for the control of wildlife populations is essential to the avoidance of human–wildlife conflict, our findings show that outbreaks of infectious disease can aggravate conflict. Governments, farmers and hunters need to improve population control after outbreaks of infectious disease.</div></div>","PeriodicalId":19577,"journal":{"name":"One Health","volume":"20 ","pages":"Article 100988"},"PeriodicalIF":4.1,"publicationDate":"2025-02-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143322936","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Antimicrobial use regulations are associated with increased susceptibility among bovine Salmonella isolates from a U.S. surveillance system","authors":"Claudia Cobo-Angel ,&nbsp;Maya Craig ,&nbsp;Marwan Osman ,&nbsp;Kevin J. Cummings ,&nbsp;Casey L. Cazer","doi":"10.1016/j.onehlt.2025.100983","DOIUrl":"10.1016/j.onehlt.2025.100983","url":null,"abstract":"<div><div>Health authorities around the world have called to limit antimicrobial use in food-producing animals. In the United States, two recent regulatory actions have changed the use of antimicrobials in livestock, banning production uses in 2017 and restricting extra-label use of cephalosporins in 2012. This study aimed to assess the impact of the 2012 and 2017 regulations on antimicrobial use in cattle in the United States by analyzing 18,627 bovine <em>Salmonella</em> AMR susceptibility patterns using data from the National Antimicrobial Resistance Monitoring System (NARMS). Logistic regression was used to model the odds of being a susceptible isolate. Additionally, interval-censored accelerated failure time (AFT) models were used to analyze changes in minimum inhibitory concentrations (MICs) over time and by serotype. The most common serotypes were Montevideo (<em>n</em> = 3003), Anatum (<em>n</em> = 1394), Cerro (<em>n</em> = 1373), and Typhimurium (<em>n</em> = 1213). Susceptibility was highest for azithromycin (99 %), ciprofloxacin, gentamicin, and trimethoprim-sulfamethoxazole (all 98 %), and lowest for tetracycline (76 %), chloramphenicol (86 %), and ampicillin (85 %). Serotypes Typhimurium, Newport, and Dublin exhibited lower susceptibility compared to other serotypes. Susceptibility to all antimicrobials increased during the periods 2013–2017 and 2018–2022 compared to isolates before 2012, with a greater increase in 2018–2022. MICs decreased for most antimicrobials except for chloramphenicol and gentamicin, which showed increased median MIC for the periods 2013–2017 and 2018–2022, respectively. In conclusion, antimicrobial use restrictions appear correlated with a reduction in <em>Salmonella</em> AMR, although this effect cannot be untangled from the effect of time in this dataset.</div></div>","PeriodicalId":19577,"journal":{"name":"One Health","volume":"20 ","pages":"Article 100983"},"PeriodicalIF":4.1,"publicationDate":"2025-01-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143322937","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Profiles and predictors of access to human and veterinary healthcare in multispecies households","authors":"Jennifer W. Applebaum ,&nbsp;Courtney Dunn ,&nbsp;Shelby E. McDonald ,&nbsp;Kaylinn Escobar ,&nbsp;Erin K. King ,&nbsp;Rosalie Corona ,&nbsp;Megan K. Mueller","doi":"10.1016/j.onehlt.2025.100981","DOIUrl":"10.1016/j.onehlt.2025.100981","url":null,"abstract":"<div><div>This study extends a behavioral-ecological framework for healthcare access and utilization to explore patterns of healthcare and veterinary care access within pet-owning households in the United States. Using Latent Class Analysis, a person-centered analytic approach, we identified five subgroups of pet owners in a diverse national sample (<em>n</em> = 750), each characterized by unique patterns of perceived access and actual usage of both human and veterinary healthcare. The first subgroup, “Good access/unfair system” (27%) and the second subgroup, “Good access/fair system” (30%) reported high probabilities of good healthcare and veterinary care access and limited financial burden but differed in their perceptions of healthcare fairness. The third subgroup, “Good access/Medicare” (14%), primarily comprised of older adults with Medicare, reported good access to both human and veterinary care and limited financial burden, underscoring Medicare's role in stable healthcare access. The fourth subgroup, “Moderate access” (11%), characterized by financial barriers despite non-employer health insurance, showed a lower likelihood of recent dental and veterinary visits. The fifth subgroup, “Poor access” (18%), with the poorest healthcare access and highest probability of Medicaid or no insurance, highlighted significant inequalities in healthcare and veterinary care access. Sociodemographic and social environmental factors were associated with subgroup membership. For example, the groups with better access were likely to have low financial fragility (Good access/unfair system OR = 4.61, <em>p</em> <em>&lt;</em> 0.001), and those with poorer access were unlikely (Poor access OR = 0.14). Additionally, the groups with better access were less likely to experience discrimination (Good access/fair system OR = 0.58, <em>p</em> <em>&lt;</em> 0.001) and those with poorer access were more likely (Moderate and Poor access both ORs = 1.37, <em>p</em> <em>&lt;</em> 0.001). These findings emphasize the need for policies addressing factors such as economic inequality and discrimination to improve healthcare and veterinary care access and utilization. Tailored interventions at individual and systemic levels are also suggested (i.e., the Colorado “Peticaid” proposal and the Seattle One Health Clinic) to mitigate disparities and enhance healthcare and veterinary care access for vulnerable populations.</div></div>","PeriodicalId":19577,"journal":{"name":"One Health","volume":"20 ","pages":"Article 100981"},"PeriodicalIF":4.1,"publicationDate":"2025-01-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143175757","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Anti-phenolic glycolipid antibodies in Mycobacterium bovis infected cattle","authors":"Zijie Zhou ,&nbsp;Anouk van Hooij ,&nbsp;J. Hessel M. van Dijk ,&nbsp;Nina Musch ,&nbsp;Louise Pierneef ,&nbsp;Hamza Khalid ,&nbsp;Kees Franken ,&nbsp;Thomas Holder ,&nbsp;Neil Watt ,&nbsp;Anita L. Michel ,&nbsp;Jeroen D.C. Codée ,&nbsp;Martin Vordermeier ,&nbsp;Paul L.A.M. Corstjens ,&nbsp;Elisabeth M.D.L. van der Heijden ,&nbsp;Jayne C. Hope ,&nbsp;Annemieke Geluk","doi":"10.1016/j.onehlt.2025.100982","DOIUrl":"10.1016/j.onehlt.2025.100982","url":null,"abstract":"<div><div><em>Mycobacterium bovis</em>, the causative agent of bovine tuberculosis (bTB), causes significant financial losses in the agricultural industry. Additionally, <em>M. bovis</em> transmission from animals to humans can result in zoonotic TB, especially in low- and middle-income countries (LMICs), highlighting the need to enhance One Health surveillance to mitigate this threat.</div><div>Antibodies directed against a major mycobacterial cell wall component of <em>M. leprae</em>, phenolic glycolipid-I (PGL-I), have shown excellent performance in identifying <em>M. leprae</em> infection in humans and animals. In this study, we therefore investigated whether antibodies against <em>M. bovis</em> PGL similarly represent a useful biomarker for <em>M. bovis</em> infection in cattle.</div><div>Comparing sera from naturally <em>M. bovis</em>-infected and the single intradermal comparative cervical tuberculin test (SICCT)-negative cattle, we assessed the potential of <em>M. bovis</em> PGL antibodies to identify this mycobacterial infection. Our results show that serum levels of anti-<em>M. bovis</em> PGL IgG and -IgM in <em>M. bovis</em>-infected cattle were significantly higher than in the SICCT-negative cattle. The sensitivity for anti-<em>M. bovis</em> PGL IgM in infected animals was, however, moderate (44.9 %) and the false-positive rate was 6.3 % in SICCT-negative cattle. Notably, vaccination with BCG- or heat-killed <em>M. bovis</em> did not affect serum levels of anti-<em>M. bovis</em> PGL IgM in cattle. Moreover, none of the 57 anti-<em>M. bovis</em> PGL-seropositive cattle tested positive in the anti-<em>M. leprae</em> PGL-I assay. This study shows for the first time that anti <em>M. bovis</em> PGL antibodies can be detected in infected cattle: anti-<em>M. bovis</em> PGL IgM is a highly specific, but moderately sensitive biomarker for <em>M. bovis</em> infection in cattle, showing potential for differentiate infected from vaccinated animals (DIVA). It could be a valuable component in a multi-biomarker approach for diagnosing bTB.</div></div>","PeriodicalId":19577,"journal":{"name":"One Health","volume":"20 ","pages":"Article 100982"},"PeriodicalIF":4.1,"publicationDate":"2025-01-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143175756","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Shaping the human gut microbiota: The role of canine companionship, lifestyle choices, and Blastocystis sp.","authors":"Zuzana Pavlíčková ,&nbsp;Barbora Pafčo ,&nbsp;Vladislav Ilík ,&nbsp;Lee O'Brien Andersen ,&nbsp;Milan Jirků ,&nbsp;Kristýna Brožová ,&nbsp;David Modrý ,&nbsp;Oldřiška Kadlecová ,&nbsp;Christen Rune Stensvold ,&nbsp;Kateřina Jirků","doi":"10.1016/j.onehlt.2025.100979","DOIUrl":"10.1016/j.onehlt.2025.100979","url":null,"abstract":"<div><div>External factors affecting composition of the human gut microbiota have attracted considerable attention in recent years. Among these factors, habitat sharing with other humans and companion animals, especially dogs, is considered crucial together with the presence of intestinal protists. The Czech Republic, known for one of the highest rates of dog ownership in Europe, provides an ideal setting for studying such relationships.</div><div>Here, we investigated the impact of dog ownership and lifestyle factors (residing in cities versus villages) on the gut microbiota (specifically bacteriome). In addition, we also investigated the influence of the common gut protist <em>Blastocystis</em> sp. on the human gut microbiota. Fecal DNAs from 118 humans and 54 dogs were subject to 16S rRNA gene sequencing using the Illumina MiSeq platform.</div><div>Greater microbial diversity was observed in humans than in dogs. Owning a dog had no significant effect on the alpha and beta diversity of the human microbiota, although some bacterial genera were enriched in dog owners. In relation to lifestyle, urban dwellers had higher levels of <em>Akkermansia</em>, while people living in villages had a more diverse gut microbiota. The presence of <em>Blastocystis</em> sp. in humans correlated with specific microbial patterns, indicating an important role for this micro-eukaryote in the gut ecosystem.</div><div>These findings highlight the intricate relationship between specific factors and the gut microbiota composition and emphasize the need for more extensive research in this area.</div></div>","PeriodicalId":19577,"journal":{"name":"One Health","volume":"20 ","pages":"Article 100979"},"PeriodicalIF":4.1,"publicationDate":"2025-01-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143175755","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}