One Health最新文献

{"title":"Rapid serotype-independent differential detection of biofilm-positive and biofilm-negative Salmonella using Fourier transform infrared biotyping","authors":"Asmita Shrestha,&nbsp;Smriti Shringi,&nbsp;Devendra H. Shah","doi":"10.1016/j.onehlt.2025.101004","DOIUrl":"10.1016/j.onehlt.2025.101004","url":null,"abstract":"<div><div>Foodborne illnesses caused by <em>Salmonella</em> represent a global one health challenge, with biofilm-forming strains exhibiting enhanced public health risks due to their ability to persist due to resistance to antimicrobial agents, disinfectants, and environmental stresses. While food-safety and public health investigation primarily focus on <em>Salmonella</em> identification and source tracing, they often overlook the biofilm-forming capacity of isolates, limiting their predictive value for risks posed by biofilm producing <em>Salmonella</em>. This study assessed fourier transform infrared (FTIR) biotyping for rapid serotype-independent differentiatial detection of biofilm-positive (BFP) from biofilm-negative (BFN) <em>Salmonella</em>. A total of 270 <em>Salmonella</em> strains representing 12 common serotypes were classified using three conventional biofilm assays (congo red and coomassie brilliant blue agar test, calcofluor test, and tube test) into true BFP (<em>n</em> = 80), true BFN (<em>n</em> = 64), and uncertain (<em>n</em> = 59) biofilm producers. Biofilm production for each group was also assessed with a microtiter plate assay. FTIR biotyping was applied to a subset of 115 strains (61 BFP, 54 BFN). Using spectral windows of 1180–1050 cm⁻¹ and 1400–1200 cm⁻¹, FTIR biotyping accurately differentiated BFP from BFN strains with 93.4 % sensitivity, 83.3 % specificity, and 88.6 % overall accuracy. FTIR biotyping differentiated 59 strains with uncertain biofilm status into BFN (<em>n</em> = 45) and BFP (<em>n</em> = 14). FTIR biotyping provides a rapid, sensitive and specific method for detection of biofilm-forming <em>Salmonella</em> strains. Incorporating FTIR biotyping for biofilm detection in current <em>Salmonella</em> surveillance and source-tracing protocols can enhance food safety risk assessments and improve <em>Salmonella</em> outbreak prevention.</div></div>","PeriodicalId":19577,"journal":{"name":"One Health","volume":"20 ","pages":"Article 101004"},"PeriodicalIF":4.1,"publicationDate":"2025-03-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143579565","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Assessing risk factors for foodborne illness in restaurants in Seoul","authors":"Joowon Lee,&nbsp;Sooyoung Huh","doi":"10.1016/j.onehlt.2025.101009","DOIUrl":"10.1016/j.onehlt.2025.101009","url":null,"abstract":"<div><div>Restaurants are the most common locations for foodborne infectious disease outbreaks in Korea, accounting for almost half of the annual outbreaks nationwide. Although significant efforts have been made to reduce the incidence of foodborne illnesses, factors contributing to these outbreaks have not been adequately elucidated. The purpose of this study was to assess the risk factors for foodborne illnesses in restaurants in Seoul using the recommendations of the U.S. Food and Drug Administration. We used data from a survey conducted by the Seoul Metropolitan Government as part of a 2024 foodborne infectious disease control project. In the survey, of all registered restaurants in Seoul, 886 were selected and invited to participate using a systematic sampling method, and 400 voluntarily participated. The results showed that 10.3 % of restaurants had employee health policies for managing ill food workers, 8.3 % had a food hygiene certificate granted by the government, and 37.5 % provided paid sick leave. Additionally, the provision rate of guidance for handwashing and glove use was found to be low. Therefore, restaurants in Seoul were considered at an increased risk of foodborne infectious diseases. The study findings highlight the gaps in foodborne infectious disease control systems in restaurants in Seoul. Science-based customized approaches are required for controlling foodborne illnesses.</div></div>","PeriodicalId":19577,"journal":{"name":"One Health","volume":"20 ","pages":"Article 101009"},"PeriodicalIF":4.1,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143550668","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Integrated opisthorchiasis control through the EcoHealth/one health approach: 15 years of success and experiences with the Lawa model","authors":"Banchob Sripa ,&nbsp;Sirikachorn Tangkawattana ,&nbsp;Mingkwan Sangnikul","doi":"10.1016/j.onehlt.2025.101006","DOIUrl":"10.1016/j.onehlt.2025.101006","url":null,"abstract":"<div><div><em>Opisthorchis viverrini</em> infection remains a major health problem in Northeast Thailand and the Mekong region impacting over 12 million and causing bile duct cancer. Using an EcoHealth/One Health approach at Lawa Lake in Thailand, our integrated control program achieved a substantial reduction in liver fluke prevalence from 60 % to &lt;5 % over 15 years. Key interventions included chemotherapy, collaboratively designed health education, ecosystem modification, and community participation. Infections in intermediate hosts, <em>Bithynia</em> snails and Cyprinoid fish, are now undetectable. Improved community knowledge resulted in healthier practices. The “Lawa Model”, a recognized model for liver fluke control, is now a training hub being scaled up in Thailand and the Mekong region. This program demonstrates how One Health strategies can address complex health and ecological challenges and aligns with WHO recommendations. The success of the Lawa Model demonstrates the efficacy of integrated One Health interventions against endemic parasitic diseases.</div></div>","PeriodicalId":19577,"journal":{"name":"One Health","volume":"20 ","pages":"Article 101006"},"PeriodicalIF":4.1,"publicationDate":"2025-02-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143550670","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Interactions between microplastics and microbiota in a One Health perspective","authors":"Anna Caterina Procopio ,&nbsp;Alessio Soggiu ,&nbsp;Andrea Urbani ,&nbsp;Paola Roncada","doi":"10.1016/j.onehlt.2025.101002","DOIUrl":"10.1016/j.onehlt.2025.101002","url":null,"abstract":"<div><div>Microplastics are recognised as ubiquitous pollutants as they are now found in all terrestrial and marine ecosystems. The interactions between microbiota and microplastics are an issue of fundamental importance in studying and maintaining global health. Microplastics alter the structures and functions of microbial communities, resulting in adverse health effects. A comprehensive understanding of these effects through interdisciplinary research is essential to mitigate pollution and protect the health of ecosystems. The review aims to explore these interactions within a One Health framework. Indeed, a deeper understanding of the processes involved in the interaction between microbiota and microplastics could pave the way for new and promising strategies to mitigate the harmful effects of microplastics on ecosystems and human health.</div></div>","PeriodicalId":19577,"journal":{"name":"One Health","volume":"20 ","pages":"Article 101002"},"PeriodicalIF":4.1,"publicationDate":"2025-02-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143550671","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Challenges and opportunities for AMR research in the ASEAN following the One Health approach","authors":"Harish Kumar Tiwari ,&nbsp;Daniel K.Y. Tan ,&nbsp;Chhe Chinda ,&nbsp;Duong Nu Tra My ,&nbsp;Ha Thi Thu Hoang ,&nbsp;Khao Keonam ,&nbsp;Luu Quynh Huong ,&nbsp;Ly Chanvatanak ,&nbsp;Mot Virak ,&nbsp;Nguyen Thuy Tram ,&nbsp;Nittakone Soulinthone ,&nbsp;Pham Duc Phuc ,&nbsp;Thi Thu Hoai Nguyen ,&nbsp;Vu Thi Thu Tra ,&nbsp;Justin Beardsley","doi":"10.1016/j.onehlt.2025.101001","DOIUrl":"10.1016/j.onehlt.2025.101001","url":null,"abstract":"<div><h3>Background</h3><div>Antimicrobial resistance (AMR) has emerged as a significant global challenge and Southeast Asia with rapid economic and population growth faces substantial challenge in dealing with emerging infectious diseases and antimicrobial resistance. Here we present the recommendations of a workshop that explored the challenges and opportunities for One Health approach towards AMR research in three countries of AEAN, namely, Cambodia, Laos, and Vietnam.</div></div><div><h3>Methods</h3><div>A workshop was organised in Hanoi, Vietnam in August 2023, involving participants involved in AMR research across varied sectors from three participating countries to prioritise the strategies that can be implemented in the region to fructify the One Health approach to tackle AMR. A modified Delphi approach was used to prioritise the top 10 Global Priority Research Questions for the region as developed by the Quadripartite (FAO, WHO, WOAH and UNEP). An iterative process was adopted to map priorities according to their impact and feasibility of application.</div></div><div><h3>Results</h3><div>Collaborative initiatives, such as a common platform for listing the research goals, a web-based surveillance mechanism, and an enhanced AMR awareness curricula were identified as the steps forward. A consensus statement highlighting the critical needs for improved technical and infrastructure capacity, collaboration between sectors, increased funding, and systematic data analysis was drafted.</div></div><div><h3>Discussion</h3><div>The participating countries have National Action Plans guided by the World Health Organization's Global Action Plan on AMR, but limited collaboration between human health and other sectors has impeded the benefits that One Health approach may achieve in the region. The recommendations include the need for improved technical and infrastructure capacity, and data collection across One Health sectors, besides increasing awareness at multiple levels.</div></div><div><h3>Conclusion</h3><div>A collaborative and coordinated effort to apply One Health initiatives for tackling AMR in the ASEAN region is imperative. The workshop formulated a roadmap for future direction by identifying priorities aimed at enhancing collaboration, addressing infrastructure gaps, and contributing to an effective intervention in the fight against AMR in the region.</div></div>","PeriodicalId":19577,"journal":{"name":"One Health","volume":"20 ","pages":"Article 101001"},"PeriodicalIF":4.1,"publicationDate":"2025-02-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143550669","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Insights and future directions: Applying the One Health approach in international agricultural research for development to address food systems challenges","authors":"Hung Nguyen-Viet ,&nbsp;Steven Lâm ,&nbsp;Silvia Alonso ,&nbsp;Fred Unger ,&nbsp;Arshnee Moodley ,&nbsp;Bernard Bett ,&nbsp;Eric M. Fèvre ,&nbsp;Theodore Knight-Jones ,&nbsp;Siobhan M. Mor ,&nbsp;Ha Thi Thanh Nguyen ,&nbsp;Delia Grace","doi":"10.1016/j.onehlt.2025.101007","DOIUrl":"10.1016/j.onehlt.2025.101007","url":null,"abstract":"<div><div>For more than 15 years, the International Livestock Research Institute (ILRI) has been striving to understand and address One Health challenges at the intersection of livestock, humans, and the environment. We present an overview of ILRI One Health projects implemented with partners across Asia and Africa, reflecting on key learnings and future directions for One Health research and food systems transformation. Drawing on a review of peer-reviewed and grey literature, we analyzed processes and outcomes of ILRI-led and supported initiatives using a realist evaluation framework (context, mechanisms, outcomes), and present insights within select One Health topic areas such as zoonoses, food safety, antimicrobial resistance. Our findings emphasize the need for stronger cross-sectoral collaboration, greater engagement with policymakers to translate research findings into actionable strategies, and the development of adaptable and context-specific interventions.</div></div>","PeriodicalId":19577,"journal":{"name":"One Health","volume":"20 ","pages":"Article 101007"},"PeriodicalIF":4.1,"publicationDate":"2025-02-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143550672","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evaluation of a modified IDEXX method for antimicrobial resistance monitoring of extended Beta-lactamases-producing Escherichia coli in impacted waters near the U.S.-Mexico border","authors":"Karina Jimenez ,&nbsp;Yuwei Kong ,&nbsp;Yuhui Zhang ,&nbsp;Drew Ferketic ,&nbsp;Sana K. Nagori ,&nbsp;Julie Yang ,&nbsp;Anastasia A. Yulo ,&nbsp;Brianna Kramer ,&nbsp;Ofelia G. Prado ,&nbsp;Taylor Cason ,&nbsp;Renee Chowdhry ,&nbsp;Angela Kemsley ,&nbsp;Leopoldo Mendoza Espinosa ,&nbsp;Joshua A. Steele ,&nbsp;John Griffith ,&nbsp;Jennifer A. Jay","doi":"10.1016/j.onehlt.2025.100997","DOIUrl":"10.1016/j.onehlt.2025.100997","url":null,"abstract":"<div><div>As part of a One Health approach, the World Health Organization (WHO) has deemed extended beta-lactamases-producing <em>Escherichia coli</em> (ESBL-Ec) as an appropriate proxy for antimicrobial resistance (AMR) in human, animal, and environmental samples. Traditional methods for ESBL-Ec quantification involve a labor-intensive process of membrane filtration, culturing in the presence and absence of antibiotics, and colony confirmation. The emerging modified IDEXX method utilizes IDEXX Colilert-18 test kits, recognized by the USEPA for the enumeration of total coliforms and <em>E. coli</em> in water samples, modified with cefotaxime for measurement of ESBL-Ec in environmental samples. However, this method has yet to be validated for ocean or sewage-contaminated water and has not been compared against the plate-based method with mTEC for surface water. In this study, ESBL-Ec in ocean and river waters of the Tijuana River Estuary were analyzed by three methods: membrane filtration using mTEC plates (as outlined in USEPA Method 1603), membrane filtration using TBX plates (as outlined in the WHO Tricycle Protocol), and Colilert-18 spiked with cefotaxime (Hornsby et al., 2023). Levels of ESBL-Ec were elevated in the Tijuana River Estuary and nearby ocean samples, as high as 2.2 × 10⁶ CFU/100 mL. The modified IDEXX method correlated with membrane filtration methods using selective mTEC (<em>r</em> = 0.967, <em>p</em> &lt; 0.001, <em>n</em> = 14) and TBX (<em>r</em> = 0.95, p &lt; 0.001, n = 14) agars. These results indicate that the modified IDEXX method can be used as a more accessible alternative to the traditional culturing methods as a screening tool for antibiotic resistance in urban aquatic environments. Advantages of the IDEXX-based method including portability, lower Biosafety Level requirements, fewer dilutions to stay within the dynamic range, greater ease of maintaining sterility during analysis, and less required staff training are discussed. Future studies into the validity of the modified IDEXX method compared to qPCR and metagenomic sequencing are needed.</div></div>","PeriodicalId":19577,"journal":{"name":"One Health","volume":"20 ","pages":"Article 100997"},"PeriodicalIF":4.1,"publicationDate":"2025-02-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143563593","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Seroprevalence of influenza A H1N1 and influenza D viruses in ruminants in Qatar","authors":"Hadeel T. Zedan ,&nbsp;Tasnim Alziftawi ,&nbsp;Abrar Abdalla ,&nbsp;Hadi M. Yassine","doi":"10.1016/j.onehlt.2025.101005","DOIUrl":"10.1016/j.onehlt.2025.101005","url":null,"abstract":"<div><h3>Background</h3><div>Influenza is among the most common viruses affecting humans and many animals worldwide. While influenza A (IAV) and D (IDV) viruses are associated with respiratory disease in humans and animals, respectively, their prevalence in the Middle East is unknown.</div></div><div><h3>Methods</h3><div>Here, we assessed the seroprevalence of IDV and IAV/H1N1 in 331 ruminants (including camels, sheep, cattle, and goats) in Qatar. Sera samples were collected from ruminants in different farms and titrated by hemagglutination inhibition (HAI) assay.</div></div><div><h3>Results</h3><div>We showed a high prevalence of IDV in all ruminants, ranging between 55 and 84 %, with the highest rates seen in sheep and cattle. The rates were much lower for IAV, ranging between 6 and 8 %, but were not detectable in goats. HAI titers of IDV-seropositive samples ranged between 20 and 2560, whereas IAV titers ranged between 20 and 640.</div></div><div><h3>Conclusions</h3><div>Our study provides the first serological evidence of IDV and IAV/H1N1 in ruminants in Qatar. These results underscore the need for further investigation into the role of ruminants in influenza virus transmission.</div></div>","PeriodicalId":19577,"journal":{"name":"One Health","volume":"20 ","pages":"Article 101005"},"PeriodicalIF":4.1,"publicationDate":"2025-02-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143518946","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Mpox alert: COVID-19's hard-earned lessons put to the test","authors":"Babatope O. Adebiyi","doi":"10.1016/j.onehlt.2025.101003","DOIUrl":"10.1016/j.onehlt.2025.101003","url":null,"abstract":"","PeriodicalId":19577,"journal":{"name":"One Health","volume":"20 ","pages":"Article 101003"},"PeriodicalIF":4.1,"publicationDate":"2025-02-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143579566","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Going batty: US bat imports raise concerns for species conservation and human health","authors":"Mayla Tarango ,&nbsp;Jonathan E. Kolby ,&nbsp;Orion L.B. Goodman ,&nbsp;C. Jane Anderson ,&nbsp;Jen Tinsman ,&nbsp;Jason Kirkey ,&nbsp;Amanda Liew ,&nbsp;Marshall Jones ,&nbsp;Cadhla Firth ,&nbsp;Jamie K. Reaser","doi":"10.1016/j.onehlt.2025.100999","DOIUrl":"10.1016/j.onehlt.2025.100999","url":null,"abstract":"<div><div>The relationship between bats (order: Chiroptera) and humans is complex, highly contextualized across the globe, and dynamic. We assessed the United States (US) importation of live bats, as well as bat-derived products, from bat conservation and human health (zoonotic disease) perspectives. From 2015 to 2023, at least 284 bat species were imported into the US from at least 106 countries of origin, predominantly Indonesia. Scientific use was the most frequently declared import purpose. According to the IUCN Red List, thirty-seven of these bat species are extinction vulnerable. An investigation of US-based e-commerce platforms for live bats and bat-derived products located ads for 28 bat species for sale, including one for which trade is prohibited. Considering zoonotic spillover risk, we identified host-pathogen associations between 106 reported bat species imports and 45 zoonotic RNA viruses, representing 11 viral families. Twenty-five of the 45 viruses for which we found bat associations are listed as pathogens of concern by the World Health Organization or by US federal agencies. Rabies is the most hosted virus among bat species in our studies, followed by Dengue virus. We discuss the implications of our findings, as well as make recommendations for improving the science and policy necessary to mitigate trade-driven risks to bat and human populations. There is a need for ongoing assessments of bat population viability, as well as pathogen surveillance along the bat trade pathway. Greater attention by regulatory agencies is warranted to mitigate bat conservation and zoonotic pathogen risks apparent in e-commerce pathways. The Convention on the International Trade in Endangered Species of Fauna and Flora (CITES) and other multilateral environmental agreements could bring this issue to the forefront of member countries as part of the emerging global agenda at the conservation-zoonotic disease interface.</div></div>","PeriodicalId":19577,"journal":{"name":"One Health","volume":"20 ","pages":"Article 100999"},"PeriodicalIF":4.1,"publicationDate":"2025-02-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143510179","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}