One Health最新文献

{"title":"Molecular detection and characterization of human bocavirus in environmental waters in Thailand from 2020 to 2022","authors":"Kattareeya Kumthip ,&nbsp;Pattara Khamrin ,&nbsp;Arpaporn Yodmeeklin ,&nbsp;Hiroshi Ushijima ,&nbsp;Niwat Maneekarn","doi":"10.1016/j.onehlt.2025.101092","DOIUrl":"10.1016/j.onehlt.2025.101092","url":null,"abstract":"<div><div>Human bocavirus (HBoV) is a globally recognized pathogen associated with respiratory and gastrointestinal infections in infants and young children. Its detection in both clinical and environmental water samples has raised concerns about potential waterborne transmission. This study investigated the prevalence and genetic diversity of HBoV in environmental water sources in Chang Mai, Thailand, from July 2020 to December 2022. A total of 600 water samples from various environmental sources were analyzed using PCR assay. Nucleotide sequencing and phylogenetic analysis were performed to identify HBoV genotypes and assess their genetic characteristics. HBoV was detected in 32 of 600 samples (5.3 %), representing a notable decline in the detection rates during the COVID-19 pandemic. Among the sample types, wastewater showed highest detection rate (23.3 %), followed by irrigation water (1.2 %), and river water (0.8 %). No HBoV was detected in environmental reservoir water samples. All four known HBoV genotypes (HBoV1-HBoV4) were identified, with HBoV2 being the most prevalent genotype (19 strains; 59.4 %), followed by HBoV3 (6 strains; 18.8 %), HBoV1 (5 strains; 15.6 %), and HBoV4 (2 strains; 6.2 %). No clear seasonal pattern of HBoV contamination was observed. Phylogenetic analysis revealed a close genetic relationship between environmental and human-associated HBoV strains, suggesting an interactive transmission of HBoV between human and the environment. This study highlights the prevalence and genetic diversity of HBoV in environmental waters during the COVID-19 pandemic and underscores the need for continued surveillance to a better understanding its environmental dynamics and potential public health implications.</div></div>","PeriodicalId":19577,"journal":{"name":"One Health","volume":"20 ","pages":"Article 101092"},"PeriodicalIF":4.1,"publicationDate":"2025-05-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144167415","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Impact and lag effects of meteorology, air pollution and their interaction on HFRS: A case study of cities in Central China","authors":"Wentao Kuang ,&nbsp;Yuxi He ,&nbsp;Piaoyi Luo ,&nbsp;Liuyi Fu ,&nbsp;Han Ni ,&nbsp;Jin Zhao ,&nbsp;Shanghui Yi ,&nbsp;Wenting Zha ,&nbsp;Yuan Lv","doi":"10.1016/j.onehlt.2025.101088","DOIUrl":"10.1016/j.onehlt.2025.101088","url":null,"abstract":"<div><h3>Objective</h3><div>China has the highest incidence of hemorrhagic fever with renal syndrome (HFRS) worldwide. This study examines the influences of meteorology, air pollution, and their interplay on HFRS cases, aiming to guide prevention and control strategies under varying meteorological conditions.</div></div><div><h3>Methods</h3><div>Data on daily HFRS cases, meteorology, and air pollutants in Hunan, 2012–2021, were collected. The effects of the mixed effects of meteorological and air pollutants on the incidence of HFRS were comprehensively evaluated by constructing a distributed hysteresis linear model (DLNM) and Bayesian nuclear machine regression (BKMR).</div></div><div><h3>Results</h3><div>(1) The results of the DLNM revealed that when the wind speed (WS) = 1.9 m/s, HFRS had the lowest risk of onset (RR = 0.25). The risk of HFRS was highest on day 21 in the high WS group (RR = 1.12). (2) The results of the BKMR showed that, under medium- and low-temperature conditions, relative humidity (RH), sulfur dioxide (SO₂), and ozone (O₃) had the greatest impact (Posterior inclusion probabilities (PIP) = 1.0). (3) The combined effects of meteorological factors and atmospheric pollutants were positively correlated with the incidence of HFRS under medium and high temperature conditions, while the opposite was true for medium and low temperatures.</div></div><div><h3>Conclusion</h3><div>Meteorological factors and air pollutants have a lag effect on the incidence of HFRS. The combined effects of meteorological factors and air pollutants promote the pathogenesis and spread of HFRS. The higher the level of exposure to other studied factors at moderate and high temperatures, the greater the risk of HFRS. Thus, HFRS should be focused on and precautions should be specified in medium and high temperature environments.</div></div>","PeriodicalId":19577,"journal":{"name":"One Health","volume":"20 ","pages":"Article 101088"},"PeriodicalIF":4.1,"publicationDate":"2025-05-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144147260","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Detection of two zoonotic pathogens, Seoul orthohantavirus and pathogenic Leptospira, in rats of Bamako, Mali (2021−2023)","authors":"J. Garona ,&nbsp;A. Berard ,&nbsp;C. Tatard ,&nbsp;A. Kwasiborski ,&nbsp;P. Gauthier ,&nbsp;S. Ag Atteynine ,&nbsp;V. Hourdel ,&nbsp;A. Eusebe ,&nbsp;C. Diagne ,&nbsp;V. Caro ,&nbsp;C. Brouat ,&nbsp;N. Charbonnel ,&nbsp;V. Sauvage ,&nbsp;L. Granjon ,&nbsp;G. Castel","doi":"10.1016/j.onehlt.2025.101085","DOIUrl":"10.1016/j.onehlt.2025.101085","url":null,"abstract":"<div><div><em>Seoul orthohantavirus</em> (SEOV) and pathogenic leptospires, two zoonotic agents causing similar symptoms in humans, were investigated in rat populations across several neighborhoods in Bamako, Mali. SEOV seroprevalence in brown rats (<em>Rattus norvegicus</em>) reached 14.8 %, while no infection was detected in black rats (<em>Rattus rattus)</em>. Pathogenic leptospires were found in 8.7 % of brown rats, with significant inter-neighborhood variations, while only one black rat tested positive. Viral genetic analyses suggested that SEOV strains circulating in Bamako may result from a reassortment between two SEOV lineages. These findings highlight the widespread distribution of SEOV and the localized presence of pathogenic leptospires in Bamako, emphasizing the role of brown rats as reservoirs. These results can guide municipal authorities in implementing rodent control and prevention strategies to mitigate associated public health risks in Bamako.</div></div>","PeriodicalId":19577,"journal":{"name":"One Health","volume":"20 ","pages":"Article 101085"},"PeriodicalIF":4.1,"publicationDate":"2025-05-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144147185","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"High prevalence and diversity of Leptospira pathogenic serogroups in pigs, cows and free roaming dogs from undeserved rural communities in the coastal region of Ecuador","authors":"Solon Alberto Orlando ,&nbsp;Naomi Mora-Jaramillo ,&nbsp;Ariana León-Sosa ,&nbsp;Fabiola Jiménez Valenzuela ,&nbsp;Joselyn Calderon ,&nbsp;Ariana Rivera ,&nbsp;Emma Matamba ,&nbsp;Ericka Sanchez ,&nbsp;Gabriela Macias ,&nbsp;Gabriela Martinez ,&nbsp;Ana Piña ,&nbsp;Juana Huartanga ,&nbsp;Vicente Solis ,&nbsp;Katherine Paez ,&nbsp;Patricia Sumoy Velez ,&nbsp;Manuel Gonzalez ,&nbsp;Angel Sebastián Rodríguez-Pazmiño ,&nbsp;Miguel Angel García-Bereguiain","doi":"10.1016/j.onehlt.2025.101083","DOIUrl":"10.1016/j.onehlt.2025.101083","url":null,"abstract":"<div><div>Leptospirosis is a zoonotic disease with global distribution but endemic to tropical regions that affects animal production systems. In Ecuador, leptospirosis is endemic with records of human outbreaks dated from more than one century ago, and where the role of animal reservoirs in the transmission dynamics is not well understood. In this study, we carried out a serological surveillance by Microagglutination Test with a panel of 24 serovars for 545 animals represented by pigs and cows, and free roaming dogs from undeserved rural communities in the province of Guayas (Coastal Region of Ecuador). Our results underscore a wide diversity of pathogenic serogroups with high prevalence across animal species including Bataviae, Australis, Pomoma, Canicola, Djasiman or Grippotyphosa. Moreover, a high seroprevalence for antibodies against <em>Leptospira</em> was found across all the species analyzed, with values of 87 % (CI95 %: 70,6–100) in dogs, 66.7 % (CI95 %: 53,6-79,7) in cows, and 100 % (CI95 %: 80,4–100) in pigs. Our results confirm previous finding about the complex epidemiology of leptospirosis in Ecuador, comprising multiples serogroups and animal reservoirs. Therefore, we strongly recommend incorporating an integrative One Health approach for the surveillance and prevention of leptospirosis in Ecuador.</div></div>","PeriodicalId":19577,"journal":{"name":"One Health","volume":"20 ","pages":"Article 101083"},"PeriodicalIF":4.1,"publicationDate":"2025-05-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144124047","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Roadmap for mainstreaming integrated pest management (IPM) into a climate smart one-health (CS-OH) framework","authors":"Henri E.Z. Tonnang ,&nbsp;Ghislain T. Tepa-Yotto ,&nbsp;Bonoukpoè Mawuko Sokame ,&nbsp;Jeannette K. Winsou ,&nbsp;Manuele Tamò ,&nbsp;Rousseau F. Djouaka","doi":"10.1016/j.onehlt.2025.101084","DOIUrl":"10.1016/j.onehlt.2025.101084","url":null,"abstract":"<div><div>Climate change presents significant challenges to agricultural sustainability, particularly in integrated pest management (IPM). To address these challenges, we propose a holistic and interdisciplinary conceptual framework, climate-smart One-Health (CS-OH), which integrates ecological, environmental, and socio-economic factors, considering human, animal, soil, and water health within the environment. This paper introduces a roadmap for Climate-Smart OH IPM, combining One-Health (OH) principles with climate-smart agriculture to promote sustainable pest management amid climate change. The roadmap utilizes Systems Thinking (ST) &amp; System Dynamics (SD) methodologies to comprehend complex interactions in climate-affected agricultural systems. Additionally, we provide a step-by-step implementation of Digital Twin (DT) IPM, employing virtual models and real-time data for dynamic pest management. The roadmap's significance lies in optimizing resource allocation, fostering biodiversity, mitigating climate impact, and preventing zoonotic diseases. Furthermore, promoting adaptive pest management practices enhances agricultural system resilience. Through adopting this transformative roadmap, stakeholders can actively contribute to sustainable pest management and foster a healthier world for all.</div></div>","PeriodicalId":19577,"journal":{"name":"One Health","volume":"20 ","pages":"Article 101084"},"PeriodicalIF":4.1,"publicationDate":"2025-05-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144134310","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Prioritising intervention areas for antimicrobial resistance in Nigeria's human and animal health sectors using a mixed-methods approach","authors":"Oche A. Awulu ,&nbsp;Akinbowale Jenkins ,&nbsp;Babatunde A. Balogun ,&nbsp;Emelda E. Chukwu ,&nbsp;Folorunso O. Fasina ,&nbsp;Abiodun Egwuenu ,&nbsp;Oyinlola O. Oduyebo ,&nbsp;Tajudeen A. Bamidele ,&nbsp;Simeon Cadmus ,&nbsp;Mabel K. Aworh ,&nbsp;Adewole A. Adekola ,&nbsp;Andrew P. Desbois ,&nbsp;Kennedy F. Chah ,&nbsp;Lucy A. Brunton","doi":"10.1016/j.onehlt.2025.101082","DOIUrl":"10.1016/j.onehlt.2025.101082","url":null,"abstract":"<div><div>A One Health approach is essential to prioritise intervention areas to tackle antimicrobial resistance (AMR). This study aimed to identify and evaluate critical drivers and antimicrobial stewardship (AMS) challenges within Nigeria's human and animal health sectors.</div><div>Human (22) and animal (33) health professionals in Nigeria were asked via an online questionnaire to rank priority pathogens, AMS challenges, and AMR drivers identified by subject matter experts. Descriptive statistics and the Fisher's exact test were used to evaluate differences in rankings between sectors. Subsequently, a scoping literature review of peer-reviewed research and grey literature was conducted to evaluate the evidence supporting the rankings.</div><div><em>Salmonella</em> spp. (28.5 %) and <em>Escherichia coli</em> (28.2 %) were selected as the top-ranked priority pathogens for AMR. The Fisher's exact test showed a significant association (<em>p</em> = 0.049) between profession and ranking of <em>Salmonella</em>, which was ranked higher by animal health professionals than their human health counterparts. Priority AMS challenges in both human and animal health sectors were “ease of access to over-the-counter antimicrobials” (14.9 % and 20.1 %, respectively) and “lack of awareness of AMR/AMS” (14.1 % and 20.4 %, respectively). “Lack of infection prevention and control (IPC)” (24.5 %) was the highest-ranked AMR driver across sectors. Differences were observed between the rankings human and animal health professionals gave to the challenge of access to veterinary expertise (<em>p</em> = 0.011), as medical doctors ranked this component higher than veterinarians. “Lack of IPC” (<em>p</em> = 0.022) and “environmental degradation” (<em>p</em> = 0.048) were ranked higher by medical doctors than veterinarians. Conversely, “unsanitary processes in the abattoir(s)” was ranked higher among veterinarians (<em>p</em> = 0.032). Of the 84 articles reviewed, 62 supported the rankings of AMS challenges in both sectors, while 24 captured relevant AMR drivers.</div><div>Our findings underscore the need for a One Health approach in Nigeria to improve AMS and curb AMR.</div></div>","PeriodicalId":19577,"journal":{"name":"One Health","volume":"20 ","pages":"Article 101082"},"PeriodicalIF":4.1,"publicationDate":"2025-05-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144167414","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Development and application of web-based and mobile tools for risk-driven rabies control in Thailand","authors":"Weerapong Thanapongtharm ,&nbsp;Worapan Kusakunniran ,&nbsp;Krittanat Sutassananon ,&nbsp;Chawapat Youngjitikornkun ,&nbsp;Hathaithip Wannakee ,&nbsp;Anuwat Wiratsudakul","doi":"10.1016/j.onehlt.2025.101078","DOIUrl":"10.1016/j.onehlt.2025.101078","url":null,"abstract":"<div><div>Rabies is a life-threatening zoonotic disease, with most human cases resulting from dog-mediated transmission. In Thailand, upon the detection of an index canine rabies case, local veterinary authorities are mandated to implement ring vaccination within a 5-km radius. However, the effectiveness of this fixed control zone may vary depending on local conditions. This study developed a spatial-epidemic model to simulate rabies outbreaks in four provinces representing different regions of Thailand: Chiang Rai, Surin, Chonburi, and Songkhla. The model comprised two key components—dog movement analysis and rabies transmission simulation—and was integrated into user-friendly web-based and mobile applications. The simulated outbreak areas comprehensively covered observed outbreak locations. Designed with intuitive interfaces, the applications are accessible to local authorities without technical expertise. Additionally, users can define custom control radii if they prefer to follow traditional ring vaccination protocols. While the model's accuracy depends on the quality of the input—particularly precise dog population data—it offers a more efficient alternative to conventional fixed-radius approaches. Our customized polygonal control boundaries enable more effective resource allocation, representing a significant advancement in rabies outbreak management.</div></div>","PeriodicalId":19577,"journal":{"name":"One Health","volume":"20 ","pages":"Article 101078"},"PeriodicalIF":4.1,"publicationDate":"2025-05-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144108151","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Epidemiological and molecular characterization of Enterobius vermicularis cases in Mersin, Turkey: An analysis based on mitochondrial DNA cox1 gene sequences","authors":"Harun Gülbudak ,&nbsp;Seda Tezcan Ülger ,&nbsp;Taylan Bozok ,&nbsp;Gönül Aslan","doi":"10.1016/j.onehlt.2025.101079","DOIUrl":"10.1016/j.onehlt.2025.101079","url":null,"abstract":"<div><div><em>Enterobius vermicularis</em> is among the most common intestinal helminths, especially in children. Mitochondrial <em>cox</em>1 gene analyses revealed three genotypes (A, B, C). We aimed to evaluate the epidemiological characteristics and <em>cox</em>1 genotypes of <em>E. vermicularis</em> cases diagnosed at Mersin University Hospital. Between January 2017 and December 2023, 1496 samples were examined using the cellophane tape method, and 75 positives were identified. Epidemiological data were retrospectively analyzed. DNA was isolated from 30 positive samples via the phenol–chloroform method, and nested PCR products of the <em>cox</em>1 gene were sequenced. Phylogenetic analyses were performed using GenBank sequences. In a sample of patients with gastrointestinal signs, the positivity rate for <em>E. vermicularis</em> was 5 % (75/1496). Among the positive cases, 93.3 % were pediatric, with a mean age of 8.7 ± 7.7 years, and the highest prevalence (9 %) was observed in the 5–10 age group. The most commonly reported symptoms included abdominal pain (49.3 %), perianal itching (40 %), and nocturnal drooling (33.3 %). Molecular analysis revealed that all isolates belonged to genotype B, and eight haplotypes were identified. Six haplotypes were novel, while two haplotypes were identical to sequences previously reported from Iran, Iraq, and Greece. Although the prevalence of <em>E. vermicularis</em> in our region was relatively low, it remains a significant public health concern, especially among school-aged children. Genotype B, widely reported in Europe and the Middle East, was dominant in our study population. Moreover, haplotypes in this study were identical or closely related to those from neighboring countries. Further studies from different regions of Turkey are needed for a more comprehensive epidemiological assessment.</div></div>","PeriodicalId":19577,"journal":{"name":"One Health","volume":"20 ","pages":"Article 101079"},"PeriodicalIF":4.1,"publicationDate":"2025-05-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144089249","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evaluation of country preparedness for Nipah and avian influenza zoonotic viral threats in Bangladesh","authors":"Md Mustafizur Rahman ,&nbsp;Tristan Burgess ,&nbsp;Jeffrey C. Mariner ,&nbsp;Elizabeth Gold ,&nbsp;Jonathon D. Gass","doi":"10.1016/j.onehlt.2025.101075","DOIUrl":"10.1016/j.onehlt.2025.101075","url":null,"abstract":"<div><div>Nipah and avian influenza viruses (NiV and AIV) are priority zoonotic pathogens in Bangladesh and are also important globally because of their pandemic potential. To understand current strengths, areas for improvement, and opportunities to enhance preparedness for NiV and AIV in Bangladesh, as part of the USAID STOP Spillover program, 47 relevant stakeholders were surveyed, and two country leads of the primary surveillance systems were interviewed. Data was collected focusing on four different areas: research projects, surveillance systems, laboratories, and outbreak risk management systems. Despite progress in recent years, our study reveals significant gaps in preparedness, detection, and response capacities across sectors and highlights the importance of prioritizing extended and targeted surveillance, biosafety solutions for laboratories, and early event detection for resilient defenses against these viruses using a One Health framework.</div></div>","PeriodicalId":19577,"journal":{"name":"One Health","volume":"20 ","pages":"Article 101075"},"PeriodicalIF":4.1,"publicationDate":"2025-05-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144108098","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Indoor endotoxin and house dust mite concentrations in the canine homologue of human atopic dermatitis","authors":"Yunji Song ,&nbsp;Yeji Kim ,&nbsp;Jihyun Kim ,&nbsp;Ha-Jung Kim","doi":"10.1016/j.onehlt.2025.101076","DOIUrl":"10.1016/j.onehlt.2025.101076","url":null,"abstract":"<div><h3>Background</h3><div>Household endotoxin exposure and house dust mite (HDM) allergens have been recently gaining attention for their immunological potential to affect allergic symptoms. Although dogs are spontaneous models of human atopic dermatitis (AD), there are few studies exploring their relationship. The aim of this study is to analyze the effects of HDM and endotoxin concentrations on AD in dogs and compare their immunological profiles with humans.</div></div><div><h3>Methods</h3><div>We graded the canine AD Extent and Severity Index-4 (CADESI-4), Pruritus Visual Analogue Scale (PVAS), and transepidermal water loss (TEWL) in dogs with (<em>n</em> = 42) and without AD (controls; <em>n</em> = 18). Serum immunoglobulin E (IgE), interleukin (IL)-4, IL-31, and IL-33 levels were measured. Dog owners were required to answer residential environmental questionnaires and measure the average indoor relative humidity (RH) and temperature for 48 h. Dust samples were collected from the floor and analyzed for HDM allergens including <em>Dermatophagoides farinae</em> (<em>Der f1</em>) and <em>D. pteronyssinus (Der p1</em>) allergen and endotoxin.</div></div><div><h3>Results</h3><div>Both the <em>Der f1</em> concentration and endotoxin exposure levels from the floor dust had an inverse association with TEWL in dogs with AD (<em>P</em> &lt; 0.05) but not in controls. Furthermore, the endotoxin concentration was negatively associated with the IL-4 level. No significant difference was found in endotoxin exposure and the <em>Der f1</em> concentration between dogs with AD and controls.</div></div><div><h3>Conclusions</h3><div>Indoor HDM exposure is associated with decreased TEWL, and endotoxin concentrations may contribute to the mitigation of CAD symptoms.</div></div>","PeriodicalId":19577,"journal":{"name":"One Health","volume":"20 ","pages":"Article 101076"},"PeriodicalIF":4.1,"publicationDate":"2025-05-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144071841","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}