One Health最新文献

{"title":"Capacity building for zoonosis control in China: Assessing needs and gaps by the one health lens","authors":"Ne Qiang ,&nbsp;Tianyun Li ,&nbsp;Lijun Jia ,&nbsp;Xiaoxi Zhang ,&nbsp;Xinyu Feng ,&nbsp;Kun Yin ,&nbsp;Zelin Zhu ,&nbsp;Jinjun Ran ,&nbsp;Robert Bergquist ,&nbsp;Lefei Han ,&nbsp;Xiao-Nong Zhou","doi":"10.1016/j.onehlt.2025.101231","DOIUrl":"10.1016/j.onehlt.2025.101231","url":null,"abstract":"<div><h3>Background</h3><div>Traditional sector-specific approaches are inadequate to effectively address zoonosis control at the human-animal-environment interface, whereas the One Health approach promotes multisectoral collaboration and integrated solutions. To apply this approach, competence enhancement is essential, yet there is insufficient understanding of China's capacity-building under the One Health framework, hindering targeted development of the workforce needed.</div></div><div><h3>Objective</h3><div>To assess the gaps and needs of capacity building for zoonosis control in China.</div></div><div><h3>Methods</h3><div>From December 2023 to April 2024, field investigations were conducted in two Chinese provinces with purposively sampled participants from various administrative sectors. Semi-structured interviews (40–60 minutes) were conducted, with a One Health Capacity Building Framework developed to guide both the design and analysis. Interview recordings were transcribed and analyzed through iterative thematic analysis. A unified codebook was developed through weekly consensus meetings, and higher-order themes were identified under expert supervision. The final themes were mapped onto the framework and visualized using a heatmap to identify the most prominent gaps and needs.</div></div><div><h3>Results</h3><div>In total, 77 participants were engaged, spanning the human health (44.2 %), animal health (18.2 %), environmental management (20.8 %) and multidisciplinary coordination (16.8 %) sectors. China's One Health capacity building revealed three challenges: (i) Structural fragmentation with divided responsibilities and inadequate cross-sectoral governance mechanisms; (ii) Resource disparities showing severe township-level shortages and persistent funding gaps for critical projects; and (iii) Knowledge deficits including insufficient research on emerging pathogens and training programs lacking practical collaboration components. Critical needs focused on strengthening the grass-root infrastructure, sustaining One Health training programs, and enhancing problem-solving capabilities.</div></div><div><h3>Conclusion</h3><div>The current status and needs in capacity building for zoonosis prevention in China were identified. The results would provide evidence-based recommendations to optimize prevention and control of China's zoonotic disease and advance global health security through strengthened One Health implementation.</div></div>","PeriodicalId":19577,"journal":{"name":"One Health","volume":"21 ","pages":"Article 101231"},"PeriodicalIF":4.5,"publicationDate":"2025-10-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145265194","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Serological evidence of Q fever in Tibetan sheep in Qinghai Province, China","authors":"Xiaomin Wu ,&nbsp;Chao Chen ,&nbsp;Te Ba ,&nbsp;Lin Liang","doi":"10.1016/j.onehlt.2025.101233","DOIUrl":"10.1016/j.onehlt.2025.101233","url":null,"abstract":"<div><div>Q fever, caused by the obligate intracellular bacterium <em>Coxiella burnetii</em> (<em>C. burnetii</em>), is a global zoonotic disease. No data is available about <em>C. burnetii</em> infection in Tibetan sheep in Qinghai, China. In this study, 1043 Tibetan sheep serum samples (421 females and 622 males) were collected from 8 regions in Qinghai Province and examined for antibodies against <em>C. burnetii</em> using a commercial ELISA Kit. The overall seroprevalence of <em>C. burnetii</em> infection was 5.9 % (62/1043, 95 % CI 4.5–7.4). Interestingly, <em>C. burnetii</em> infection rates in Tibetan sheep showed no statistically significant correlations with age, geographical region, and gender (<em>P</em> &gt; 0.05). The results of the present survey support the widespread presence of <em>C. burnetii</em> in Tibetan sheep in Qinghai, also providing baseline data for improving livestock management, preventing and controlling Q fever in large ruminants in the region. Further molecular epidemiological investigations are warranted to understand the epidemiology of <em>C. burnetii</em> transmission in Qinghai, China.</div></div>","PeriodicalId":19577,"journal":{"name":"One Health","volume":"21 ","pages":"Article 101233"},"PeriodicalIF":4.5,"publicationDate":"2025-10-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145265197","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Preliminary molecular detection and characterization of Bartonella in small mammals from Malaysian oil palm ecosystems","authors":"Auni Atikah AbdulHalim ,&nbsp;Nurul Aini Husin ,&nbsp;Muhammad Rasul Abdullah Halim ,&nbsp;Muhammad Haiqal Syarriman AbdulRahim ,&nbsp;Siti Nur Athirah Azman ,&nbsp;Shih Keng Loong ,&nbsp;Norhidayu Sahimin ,&nbsp;Sazaly AbuBakar ,&nbsp;Kittipong Chaisiri ,&nbsp;Serge Morand ,&nbsp;Zubaidah Ya'cob","doi":"10.1016/j.onehlt.2025.101234","DOIUrl":"10.1016/j.onehlt.2025.101234","url":null,"abstract":"<div><div>Close contact between humans and wildlife in agricultural landscapes, particularly rodents in oil palm plantations, increases the risk of zoonotic pathogen transmission, with <em>Bartonella</em> spp. emerging as important but understudied cause of febrile illness. The study investigates the presence and genetic characterization of <em>Bartonella</em> in small mammals from Malaysian oil palm plantations to assess the zoonotic risk. A total of 297 animals were trapped and examined, representing five species: <em>Rattus tanezumi</em> R3 mitotype, <em>Rattus tiomanicus</em>, <em>Rattus exulans</em>, <em>Callosciurus notatus</em>, and <em>Tupaia glis</em>. The identification of three <em>Rattus</em> group was further validated using cytochrome <em>c</em> oxidase subunit I (COI) gene analysis. DNA was extracted from the spleen and screened for <em>Bartonella</em> and <em>Rickettsia</em> using conventional PCR targeting the <em>gltA</em> gene followed by sequencing and phylogenetic analysis of positive samples for confirmation and diversity assessment. Molecular screening revealed a 2 % (6/297) prevalence of <em>Bartonella</em> species in five <em>R. tanezumi</em> R3 mitotype and one <em>R. tiomanicus</em>. Conversely, no <em>Rickettsia</em> DNA was amplified from all of the collected samples. All <em>Bartonella</em>-positive samples phylogenetically clustered with <em>Bartonella phoceensis</em> strain 16120, a genotype not currently associated with human disease. However, its detection in predominant rodent reservoir species underscores the utility of a One Health framework. Sustained surveillance using integrated approaches is essential to monitor and mitigate potential zoonotic risks emerging from evolving agricultural landscapes.</div></div>","PeriodicalId":19577,"journal":{"name":"One Health","volume":"21 ","pages":"Article 101234"},"PeriodicalIF":4.5,"publicationDate":"2025-10-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145265196","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Global distribution and genetic diversity of orthoebolaviruses: Mapping and evolutionary analysis","authors":"Nuo Cheng ,&nbsp;Run-Ze Ye ,&nbsp;Yu-Yu Li ,&nbsp;Kandeh Bassie Kargbo ,&nbsp;Li-Li Ren ,&nbsp;Wu-Chun Cao","doi":"10.1016/j.onehlt.2025.101236","DOIUrl":"10.1016/j.onehlt.2025.101236","url":null,"abstract":"<div><div><em>Orthoebolavirus</em> genus (Family: <em>Filoviridae</em>) poses an ongoing threat to both human and animal health. To better understand and address this persistent threat, we conducted a comprehensive analysis. Through a systematic investigation of orthoebolaviruses' global geographic distribution, phylogenetic relationships, and detailed examination of nonsynonymous amino acid mutations in Zaire ebolavirus (EBOV) from both human and non-human primate (NHP) hosts, we have made several key findings. Our results demonstrate that the viruses in the genus <em>Orthoebolavirus</em> primarily circulate in Africa and Asia, with a possible trend of geographic expansion to other regions. Phylogenetic analysis revealed significant evolutionary divergence among the six viral species across different hosts and temporal scales. Most notably, we identified 73 statistically significant amino acid substitution sites (all <em>p</em> &lt; 0.0001) in EBOV from both human and NHP hosts. Collectively, this multifaceted investigation offers valuable insights that will inform future research on orthoebolaviruses' pathogenesis and intervention strategies.</div></div>","PeriodicalId":19577,"journal":{"name":"One Health","volume":"21 ","pages":"Article 101236"},"PeriodicalIF":4.5,"publicationDate":"2025-10-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145265195","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evaluation of the effects of exposure to heavy metals in rescue dogs and their role as environmental biomonitors after the Brumadinho disaster, Minas Gerais, Brazil","authors":"Fabiana Sperb Volkweis ,&nbsp;Paula Rohr ,&nbsp;Bruno Stéfano Lima Dallago ,&nbsp;Henrique C.S. Silveira ,&nbsp;Andrigo Barboza De Nardi","doi":"10.1016/j.onehlt.2025.101223","DOIUrl":"10.1016/j.onehlt.2025.101223","url":null,"abstract":"<div><div>In January 2019, a mining dam collapsed in Brumadinho, Minas Gerais, Brazil. Numerous professionals from fire brigades from several states in Brazil and trained dogs worked to rescue the victims of this tragedy and maintained direct contact with the dam's tailings. To understand the consequences generated by this exposure, a cross-sectional observational study, heavy metals, cytokines and clinical signs were correlated, was carried out with the aim of identifying biological biomarkers and the presence of metals in trained dogs. Hair and serum samples from 30 rescue dogs from the Military Fire Brigade were used to analyze heavy metals, trace elements and cytokines. Among these, 20 dogs were exposed to tailings and 10 were not involved in mining disasters. Cadmium metal obtained a significant result (<em>p</em> = 0.013) when compared with the exposure to waste and a negative correlation (-0.58) with IL-15 (<em>p</em> = 0.01). The clinical signs that presented significant values were dermatological (<em>p</em> = 0.011) and locomotor (<em>p</em> = 0.003). Of the exposed dogs (6/20) 30 %, had neoplasms in the skin and reproductive system. The dogs in the exposed group showed a positive correlation (0.49) between Lead and the number of hours worked (<em>p</em> = 0.02) and a positive correlation (0.48) with Chromium (<em>p</em> = 0.03). The correlation between clinical signs, presence of metals and inflammatory cytokines indicates that dogs can be a promising tool for characterizing long-term occupational exposures and highlights the importance of biomonitoring the health of firefighters who suffer constant environmental exposures.</div></div>","PeriodicalId":19577,"journal":{"name":"One Health","volume":"21 ","pages":"Article 101223"},"PeriodicalIF":4.5,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145265789","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Potential spillover investigated by metagenome sequencing in Laikipia, Kenya reveals tick-borne pathogens and a novel bunyavirus","authors":"Maureen Kamau ,&nbsp;Koray Ergunay ,&nbsp;Brian P. Bourke ,&nbsp;Janerose Mutura ,&nbsp;Rashid Lebunge ,&nbsp;Griphin Ochieng ,&nbsp;Kimita Gathii ,&nbsp;John Waitumbi ,&nbsp;Beth Mutai ,&nbsp;James Hassell ,&nbsp;Michael E. von Fricken ,&nbsp;Dawn Zimmerman ,&nbsp;Suzan Murray ,&nbsp;Le Jiang ,&nbsp;Hsiao-Mei Liao ,&nbsp;John P. Grieco ,&nbsp;Emily G. McDermott ,&nbsp;Nicole L. Achee ,&nbsp;Yvonne-Marie Linton","doi":"10.1016/j.onehlt.2025.101226","DOIUrl":"10.1016/j.onehlt.2025.101226","url":null,"abstract":"<div><div>Tick-borne infections continue to present a global public health threat, and require a One Health approach for successful mitigation. We conducted cross-sectional tick screening utilizing an agnostic metagenomic screening strategy based on nanopore sequencing (NS), in an area spanning a range of habitats with intensified human-livestock-wildlife interactions in central Kenya. We further used targeted amplification by polymerase chain reaction (PCR) and sequence independent single primer amplification (SISPA) for confirmation and genome characterization, as necessary. We initially screened 44 ticks across pooled and individual samples belonging to seven species. Tick-associated bacteria—including spotted fever <em>Rickettsia</em> (13.3 %) and <em>Coxiella</em>-like endosymbionts, <em>Francisella turcica</em> and <em>Francisella opportunistica</em>, and tick-associated <em>Borrelia</em>—were detected in 86.6 % of samples<em>.</em> Viruses were detected in 93.3 % of samples, where Jingmen tick virus (JMTV) was observed as the most prevalent virus, detectable in 80 % of samples. A follow-up specific PCR confirmed JMTV virus detection in 75 %, associated with viral read abundance in NS. A complete JMTV genome was assembled from an <em>Amblyomma sparsum</em> tick, that displayed conserved motifs of putative structural and replication proteins. Maximum likelihood analyses placed the virus genome within a distinct clade in the proposed East African-Asian JMTV lineage. We further investigated a virus contig generated during the initial screening, with limited identities to Volzhskoe tick virus (VSTV). Subsequent NS and targeted PCR screening in an additional collection of 650 ticks from 11 species revealed a JMTV prevalence of 12.3 % in <em>Amblyomma, Hyalomma</em> and <em>Rhipicephalus</em> species. Follow-up NS and SISPA generated viral genomic segments, encoding a putative replicase and glycoprotein precursor. Pairwise comparisons and phylogeny indicated a novel virus—herein named as the Mpala tick virus—which is related to but distinct from VSTV and placed among unclassified members of <em>Bunyaviricetes</em>. In conclusion, our approach provides an effective strategy to detect a wide range of tick-borne bacteria and viruses, facilitating identification of opportunistic or endosymbiotic bacteria as well as novel viruses.</div></div>","PeriodicalId":19577,"journal":{"name":"One Health","volume":"21 ","pages":"Article 101226"},"PeriodicalIF":4.5,"publicationDate":"2025-09-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145265199","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Toxoplasma gondii in owned and stray dogs from a Northwestern region of São Paulo State, Brazil: Seroprevalence and geospatial distribution from a One Health perspective","authors":"Fernando Henrique Antunes Murata ,&nbsp;Jessica Priscilla Barboza ,&nbsp;Fernanda Follis Tasso ,&nbsp;Tainara Souza Pinho ,&nbsp;Tiago Henrique ,&nbsp;Janine Fusco Alves ,&nbsp;FAMERP Toxoplasma Research Group ,&nbsp;Carlos Alexandre Guimarães de Souza ,&nbsp;Daniel Abrahão ,&nbsp;Ubirajara Leoncy de Lavor ,&nbsp;Luiz Carlos de Mattos ,&nbsp;Chunlei Su ,&nbsp;Cinara Cássia Brandão","doi":"10.1016/j.onehlt.2025.101222","DOIUrl":"10.1016/j.onehlt.2025.101222","url":null,"abstract":"<div><h3>Background</h3><div><em>Toxoplasma gondii</em> is a coccidian parasite capable of infecting all warm-blooded animals, including humans. While most infections are asymptomatic, severe manifestations can occur in immunocompromised individuals and during pregnancy. In dogs, <em>T. gondii</em> infection is not only of clinical significance but also of considerable epidemiological importance, given their close contact with humans and their potential role as sentinels for environmental contamination.</div></div><div><h3>Methods</h3><div>Peripheral blood samples were collected from owned and stray dogs at the Zoonosis Control Center in São José do Rio Preto, northwestern São Paulo, Brazil. The presence of anti-<em>T. gondii</em> antibodies was assessed using the modified agglutination test (MAT). Risk factors were analyzed based on individual animal data and the geospatial distribution of positive cases.</div></div><div><h3>Results</h3><div>Anti-<em>T. gondii</em> antibodies were detected in 132 out of 662 (19.9 %) owned dogs and 52 out of 211 (24.6 %) stray dogs, with an overall seroprevalence of 21.1 %. Among the risk factors analyzed, age and area were significantly associated with seroprevalence in stray dogs. Geospatial analysis identified 34 hotspots and 144 coldspots throughout the city.</div></div><div><h3>Discussion</h3><div>This is the first study to assess the prevalence and spatial distribution of <em>T. gondii</em> in dogs in this region, confirming broad environmental dissemination of the parasite. The findings highlight the importance of dogs as indicators of environmental contamination and reinforce the need for integrated control strategies under a One Health approach, particularly in high-risk areas for humans and animals.</div></div>","PeriodicalId":19577,"journal":{"name":"One Health","volume":"21 ","pages":"Article 101222"},"PeriodicalIF":4.5,"publicationDate":"2025-09-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145219257","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Associations between the 2022 global mpox outbreak and multifaceted factors: A multi-geographical retrospective study","authors":"Yuxi Ge ,&nbsp;Yifei Wang ,&nbsp;Ziqin Zhou ,&nbsp;Zhirui Zhang ,&nbsp;Yunyu Tian ,&nbsp;Yun Feng ,&nbsp;Peiyi Wu ,&nbsp;Yuxin Wang ,&nbsp;Ziyan Liu ,&nbsp;Bingying Li ,&nbsp;Zengmiao Wang","doi":"10.1016/j.onehlt.2025.101224","DOIUrl":"10.1016/j.onehlt.2025.101224","url":null,"abstract":"<div><div>Unlike prior travel-related mpox cases, the 2022 outbreak of mpox virus (MPXV) clade IIb outside endemic regions posed a significant global health threat. Despite growing recognition of the One Health relevance of mpox, the factors driving this unprecedented outbreak and their quantitative effects remain not fully understood. This study aims to identify key factors across various geographical scales to inform future mpox mitigation policies within One Health framework. We built logistic regression models to assess the association of 18 covariates—including socioeconomic, demographic, and human behaviors (e.g., urbanization, sexual behavior, immunity, mobility and contact intensity) —with mpox outbreak probability in the United States, England, Brazil, and globally. We also examined temporal trends over the past decade. Our analysis revealed positive associations between mpox cases and urbanization rates (United States: <em>R</em> = 0.43; England: <em>R</em> = 0.25; Brazil: <em>R</em> = 0.52; all <em>P</em> &lt; 0.05) and the proportion of lesbian, gay, bisexual, and transgender (LGBT) individuals (United States: <em>R</em> = 0.39; England: <em>R</em> = 0.72; both <em>P</em> &lt; 0.05; Brazil: <em>R</em> = 0.26, <em>P</em> = 0.18). Conversely, smallpox vaccination coverage showed a negative association with mpox cases (United States: <em>R</em> = -0.25, <em>P</em> = 0.08; England: <em>R</em> = -0.52). Similar trends were observed globally. Mpox outbreak probability increased globally over the past decade. Our findings highlight the role of long-term human behavior changes in MPXV clade IIb outbreaks. From a One Health perspective, these results suggest that ongoing attention to behavioral factors, alongside ecological and social contexts, may help improve understanding and prediction of outbreak dynamics.</div></div>","PeriodicalId":19577,"journal":{"name":"One Health","volume":"21 ","pages":"Article 101224"},"PeriodicalIF":4.5,"publicationDate":"2025-09-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145219254","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Accessibility of veterinary health information and its association with the uptake of veterinary drug residue contaminated products among farmers in Bugesera District, Rwanda","authors":"Severin Munyampuhwe ,&nbsp;Maurice B. Silali ,&nbsp;Celestin Munyaneza ,&nbsp;Ferdinand Bizimana ,&nbsp;Ephrem Sibomana","doi":"10.1016/j.onehlt.2025.101219","DOIUrl":"10.1016/j.onehlt.2025.101219","url":null,"abstract":"<div><div>Veterinary drug residue contaminated products result from extensive use of veterinary drugs in farm animals and poor adherence to the drug withdrawal period. The uptake of veterinary drug residue contaminated products poses human health risks, including antimicrobial resistance, cancer, and birth defects, among others. This study aims to determine the accessibility of veterinary health information and the relationship of this information with livestock farmers' knowledge, attitude and practices (KAP) regarding the uptake of veterinary drug residue contaminated products in Bugesera District. A mixed-methods, cross-sectional study was conducted among 236 farmers supplying meat and milk in Bugesera District. Data were collected through surveys, interviews, and Focus Group Discussions. Descriptive statistics, including frequencies and percentages, were used to summarize the quantitative data. Fisher's exact test and crosstabulations were employed for inferential analysis. A 95 % confidence interval was applied, and statistical significance was considered at a <em>p</em> value ≤0.05. The strength of association was measured using the odds ratio (OR). Qualitative Data were analyzed using content analysis. The results showed that 65.68 % of respondents accessed veterinary health information on the uptake of veterinary drug residue contaminated products through training on meat and milk safety and 88.56 %, and 11.44 % through veterinary service delivery from private public partnerships. Inferential analysis showed that access to veterinary health information was strongly associated with the non-uptake of veterinary drug residue contaminated milk where trained respondents were 65 % less likely to deliver veterinary drug residue contaminated milk to Milk Collection Centers (MCCs), (OR = 0.35; 95 % CI = 0.18-0.68). The study found a need for more comprehensive public private partnerships in veterinary health education, as well as the implementation of other protective measures, such as improved methods of destroying condemned carcasses, to prevent and control drug residues within the One Health ecosystem.</div></div>","PeriodicalId":19577,"journal":{"name":"One Health","volume":"21 ","pages":"Article 101219"},"PeriodicalIF":4.5,"publicationDate":"2025-09-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145157432","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Freezing stress and meat juice model alter the biofilm formation ability, gene expression, and disinfectant resistance in Salmonella serotypes","authors":"Ata Kaboudari,&nbsp;Javad Aliakbarlu","doi":"10.1016/j.onehlt.2025.101220","DOIUrl":"10.1016/j.onehlt.2025.101220","url":null,"abstract":"<div><div><em>Salmonella</em>, with its ability to survive under various environmental stress conditions, can pose a potential threat to public health, food safety, and environmental contamination by increasing its capacity to form biofilms and resist antimicrobial agents. This study aimed to investigate the effects of different meat juice models (cattle, sheep and goat meat juice) and freezing time (0–30 days) on the biofilm formation ability, <em>AdrA</em> and <em>BapA</em> genes expression, and disinfectant resistance in <em>Salmonella</em> serotypes. Biofilm formation ability was measured by colony counting method, the gene expression level was evaluated using real-time quantitative polymerase chain reaction (qPCR), and resistance to acetic acid (AA) and benzalkonium chloride (BAC) was measured by determining the minimum inhibitory concentration (MIC). The most significant increase in biofilm formation ability was observed in <em>Salmonella</em> Typhimurium (<em>S</em>. Typhimurium) in goat meat juice model. <em>Salmonella</em> Enteritidis (<em>S.</em> Enteritidis) in the cattle meat juice model exhibited a 12-fold increase in <em>AdrA</em> gene expression. Up-regulation of <em>AdrA</em> expression (up to 11-fold) was also determined in <em>S</em>. Typhimurium in goat meat juice model. Meanwhile, <em>BapA</em> gene was up-regulated up to 4.5-fold in <em>S</em>. Typhimurium in cattle meat juice. However, goat meat juice had no significant effect on the expression of <em>BapA</em> gene in <em>Salmonella</em>. The frozen sheep meat juice significantly increased the resistance of <em>Salmonella</em> to AA and BAC. The results indicated that <em>Salmonella</em> serotypes respond significantly to the freezing period and type of meat juice, which can be useful in controlling and eliminating <em>Salmonella</em> in the food industry.</div></div>","PeriodicalId":19577,"journal":{"name":"One Health","volume":"21 ","pages":"Article 101220"},"PeriodicalIF":4.5,"publicationDate":"2025-09-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145158046","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}