One Health最新文献

{"title":"Fish are poor sentinels for surveillance of riverine antimicrobial resistance","authors":"Faina Tskhay ,&nbsp;Christoph Köbsch ,&nbsp;Alan X. Elena ,&nbsp;Johan Bengtsson-Palme ,&nbsp;Thomas U. Berendonk ,&nbsp;Uli Klümper","doi":"10.1016/j.onehlt.2025.101026","DOIUrl":"10.1016/j.onehlt.2025.101026","url":null,"abstract":"<div><div>Effective surveillance of antimicrobial resistance (AMR) in the environment is crucial for assessing the human and animal health risk of AMR pollution. Wastewater treatment plants (WWTPs) are one of the main sources of AMR pollutants discharged into water bodies. One important factor for assessing the risks associated with such pollution is the colonization potential of the resistant bacteria (ARB) and resistance genes (ARGs) from the environment into human or animal microbiomes upon exposure. This study explores whether fish can act as sentinels for surveillance of AMR pollution in general and specifically the human colonization potential of ARB in rivers impacted by WWTP effluents. Two riverine fish species, Brown trout, and European bullhead, were sampled up- and downstream a German WWTP. The two fish species were chosen due to their different lifestyles: Trout are mainly actively swimming in the water phase, while bullheads are sedentary and river sediment-associated. The bacterial microbiomes and resistomes of fish gills, skin, and feces were compared with those of the respective river water and sediment up- and downstream of the WWTP. Microbiomes of both fish mirrored the changes in river water and sediment downstream of the WWTP, with significant shifts in bacterial community composition, particularly an increase in Proteobacteria and Verrucomicrobia. However, increases in ARG abundances observed in water and sediment downstream of the WWTP were not reflected in any of the fish-associated resistomes. This indicates that while the fish microbiome is sensitive to environmental changes, resistomes of poikilothermic animals such as fish are less responsive to colonization by ARB originating from WWTPs and may not serve as effective sentinels for assessing AMR pollution and colonization risks in freshwater environments. This study highlights the complexity of using wildlife as indicators for environmental AMR pollution and suggests that other species are better suited for surveillance efforts.</div></div>","PeriodicalId":19577,"journal":{"name":"One Health","volume":"20 ","pages":"Article 101026"},"PeriodicalIF":4.1,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143746737","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"One Health approach to hemotropic mycoplasmas (hemoplasmas): Molecular detection in quilombola communities and their dogs in Brazil","authors":"Louise Bach Kmetiuk ,&nbsp;Paul Shaw ,&nbsp;Ashley Wallington ,&nbsp;Giovanni Kalempa Panazzolo ,&nbsp;Orlei José Domingues ,&nbsp;Jobin Jose Kattoor ,&nbsp;Mathew Johnson ,&nbsp;Lendro Meneguelli Biondo ,&nbsp;Rebecca Wilkes ,&nbsp;Fabiano Borges Figueiredo ,&nbsp;Giovani Marino Fávero ,&nbsp;Joanne B. Messick ,&nbsp;Andrea Pires dos Santos ,&nbsp;Alexander Welker Biondo","doi":"10.1016/j.onehlt.2025.101024","DOIUrl":"10.1016/j.onehlt.2025.101024","url":null,"abstract":"<div><div>Hemoplasmas (hemotropic mycoplasmas) are obligatory red blood cell bacteria that may infect and cause anemia in several mammalian species. IAccordingly, the present study assessed four quilombola communities during six on-field expeditions between December 2021 and March 2022 in the central-eastern Paraná State, southern Brazil. Overall, 12/208 (5.8 %) quilombola individuals were positive for hemoplasma infection by qPCR. Results of 16S ribosomal RNA gene sequencing confirmed Mycoplasma heamocanis infection in two human samples. In addition, hemoplasma infection was detected by qPCR in 19/100 (19.0 %) dogs including 16/19 (84.2 %) dogs for Candidatus Mycoplasma haematoparvum, 7/19 (3.7 %) for <em>M. haemocanis</em>, and 4/19 (21.0 %) for both. To the author's knowledge, this is the first One Health approach study of hemoplasma detection in quilombola individuals along with their dogs as vulnerable populations worldwide, with M. heamocanis detection in both human and companion animals.</div></div>","PeriodicalId":19577,"journal":{"name":"One Health","volume":"20 ","pages":"Article 101024"},"PeriodicalIF":4.1,"publicationDate":"2025-03-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143848557","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Microbiome-based surveillance of zoonotic tick-borne pathogens from urban wild boars in Barcelona, 2022–2023","authors":"Laura Carrera-Faja ,&nbsp;Elmira Ghadamnan ,&nbsp;Iris Sarmiento ,&nbsp;Jordi Manuel Cabrera-Gumbau ,&nbsp;Mariette Viladomat Jasso ,&nbsp;Josep Estruch ,&nbsp;Daniel Borràs ,&nbsp;Jaime Martínez-Urtaza ,&nbsp;Johan Espunyes ,&nbsp;Oscar Cabezón","doi":"10.1016/j.onehlt.2025.101022","DOIUrl":"10.1016/j.onehlt.2025.101022","url":null,"abstract":"<div><div>Incursions of wild animals into urban areas amplify the potential risks of zoonotic disease transmission by increasing contact between humans and animal reservoirs. Monitoring the presence of pathogens in these animals is crucial for assessing zoonotic risks but remains challenging due to the vast array of known and unknown pathogens harboured by animals. Microbiome-based approaches provide an efficient and comprehensive alternative for monitoring microbial communities and scanning the whole spectrum of bacterial pathogens. In this study, we applied this innovative conceptual framework to implement a sentinel monitoring system for investigating zoonotic tick-borne bacteria in three tick species sampled from wild boars in the Metropolitan Area of Barcelona (MAB). Using Nanopore sequencing of the full length 16 s rRNA gene, we demonstrated a fast and cost-effective approach for microbiome analysis. Our findings revealed the presence of two pathogenic genera widely documented in ticks, encompassing five species: <em>Rickettsia massiliae</em> and <em>R. slovaca</em>, both previously detected in the area, and for the first time, <em>Francisella tularensis</em> —the causative agent of tularemia— as well as <em>F. hispaniensis</em> and <em>Diplorickettsia massiliensis</em>, potentially emerging patogens. Finally, our results showed distinct bacterial compositions across the tick species examined. This study highlights the sensitivity and comprehensiveness of microbiome-based surveillance of tick-borne pathogens, enabling the early detection of emerging and low-abundance bacterial species that might otherwise go unnoticed with less sensitive techniques. Such proactive detection efforts are crucial for facilitating early identification and implementing prevention strategies to mitigate zoonotic risks.</div></div>","PeriodicalId":19577,"journal":{"name":"One Health","volume":"20 ","pages":"Article 101022"},"PeriodicalIF":4.1,"publicationDate":"2025-03-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143725533","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Sustainable control and integrated management through a one health approach to mitigate vector-borne disease","authors":"Jing Ni ,&nbsp;Jinna Wang ,&nbsp;Wenrong Zhang ,&nbsp;Enfu Chen ,&nbsp;Yi Gao ,&nbsp;Jimin Sun ,&nbsp;Wenzhong Huang ,&nbsp;Jun Xia ,&nbsp;Weiming Zeng ,&nbsp;Junxiang Guo ,&nbsp;Zhenyu Gong","doi":"10.1016/j.onehlt.2025.101018","DOIUrl":"10.1016/j.onehlt.2025.101018","url":null,"abstract":"<div><div>Vector-borne diseases have a huge negative impact globally. WHO has proposed the concepts of “sustainable control” and “integrated management” to control vector organisms. It formally proposed the “Global Response to Vector Control 2017-2030” in 2017. Combining this concept with the One Health (OH) concept, China has proposed a scalable approach to ----- Four Pest-Free village, aiming to reduce the incidence of vector-borne diseases by reducing vector density through the use of environmentally friendly treatments, thereby reducing the incidence of vector-borne diseases and achieving human, animal and environmental health. The results of the study show that the “Four Pest-Free Village” has a significant effect on controlling vector populations, transforming the rural landscape, increasing the area of wetlands and reducing the incidence of vector-borne diseases.</div><div>The response of Zhejiang Province's Four Pest-Free Village to vector-borne diseases, particularly mosquito-borne diseases, provides a proof of concept that a multisectoral effort involving community members with the concept of One Health and the GVCR as proposed by the WHO can be effective in responding to outbreaks of zoonotic diseases while also protecting the national economy and reducing national financial support for infectious diseases.</div></div>","PeriodicalId":19577,"journal":{"name":"One Health","volume":"20 ","pages":"Article 101018"},"PeriodicalIF":4.1,"publicationDate":"2025-03-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143746736","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Integrating environment into One Health approaches: Embracing the complexity","authors":"Christina Lynggaard ,&nbsp;Jan F. Gogarten ,&nbsp;Pikka Jokelainen","doi":"10.1016/j.onehlt.2025.101021","DOIUrl":"10.1016/j.onehlt.2025.101021","url":null,"abstract":"","PeriodicalId":19577,"journal":{"name":"One Health","volume":"20 ","pages":"Article 101021"},"PeriodicalIF":4.1,"publicationDate":"2025-03-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143746738","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Serological evaluation of Crimean-Congo Hemorrhagic fever in humans with High-Risk professional exposure and in residual sera collected in 2022-2023 across Corsica (France)","authors":"Paloma Kiwan ,&nbsp;Morena Gasparine ,&nbsp;Dorine Decarreaux ,&nbsp;Lisandru Capai ,&nbsp;Shirley Masse ,&nbsp;Miša Korva ,&nbsp;Tatjana Avšič-Županc ,&nbsp;Jean Canarelli ,&nbsp;Marie-Helene Simeoni ,&nbsp;Xavier de Lamballerie ,&nbsp;Remi Charrel ,&nbsp;Alessandra Falchi","doi":"10.1016/j.onehlt.2025.101020","DOIUrl":"10.1016/j.onehlt.2025.101020","url":null,"abstract":"<div><h3>Background</h3><div>The Crimean-Congo hemorrhagic fever virus (CCHFV) is a tick-borne pathogen known to cause severe viral hemorrhagic fever.</div></div><div><h3>Aim</h3><div>We aimed to evaluate the potential circulation of CCHFV in Corsica through a serosurvey, including anonymized residual sera (RS) and high-risk individuals exposed to animals and/or tick bites due to occupational activities.</div></div><div><h3>Methods</h3><div>This cross-sectional study involved two groups: anonymized RS from medical biology laboratories and sera from high-risk individuals (slaughterhouse workers, veterinary professionals, animal farmers, and rangers) collected across Corsica during 2022–2023. Antibodies targeting the CCHFV viral nucleoprotein were detected using a double-antigen ELISA. ELISA-positive samples underwent neutralizing antibody testing. Sociodemographic and epidemiological data were collected using a structured questionnaire in the high-risk group.</div></div><div><h3>Results</h3><div>Total anti-CCHFV seropositivity was of 0.08 % (<em>n</em> = 2) [95 % Confidence Interval (CI): 0.06–0.09] in RS and of 0.50 % (<em>n</em> = 1) [95 % CI: 0.43–0.56] in high-risk groups (<em>p</em> &lt; 0.01). Lifetime tick-bites was reported by 65.9 % (<em>n</em> = 118) of respondents, with higher rates among farmers (Odds Ratio (OR) = 3.4; 95 % CI 1.4–8.5) and participants with &gt;10 years of occupational exposure (OR = 3.8; 95 % CI 1.7–8.5).</div></div><div><h3>Conclusions</h3><div>This study provides initial evidence of human exposure to CCHFV in Corsica, with rates consistent with those observed in other Western European regions. Our results indicate a risk of CCHF among the Corsican population, particularly among farmers and slaughterhouse workers. Continuous surveillance and public education are essential to mitigate this risk, especially among these targeted groups and healthcare professionals, ensuring prompt diagnosis and prevention of potential outbreaks.</div></div>","PeriodicalId":19577,"journal":{"name":"One Health","volume":"20 ","pages":"Article 101020"},"PeriodicalIF":4.1,"publicationDate":"2025-03-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143714785","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Teaching inequity in vector-borne diseases management through a socioscientific issue framework","authors":"Nicole A. Scavo ,&nbsp;Sarah Poor ,&nbsp;Francisco C. Ferreira ,&nbsp;Yuexun Tian ,&nbsp;Julia Gonzalez ,&nbsp;Jordan Salomon","doi":"10.1016/j.onehlt.2025.101019","DOIUrl":"10.1016/j.onehlt.2025.101019","url":null,"abstract":"<div><div>The distribution and management of vector-borne diseases (VBD) are affected by a social and cultural environment, including forms of systemic oppression such as racism and classism. University classes that cover these diseases usually focus on teaching biological concepts while skimming over other social, economic, and cultural factors that influence them. With no current lesson plan widely available to implement, we present a lesson plan intended to be delivered at the end of courses related to disease ecology to emphasize inequity issues that impact VBD management while also serving as a capstone for the biological content taught in the class. The lesson is based on the demographic and disease risk data from local contexts to provide effective place-based and socioscientific issues instruction. The lesson is designed as a role-playing simulation where groups of students are given separate disease outbreaks to combat with specific budgets. Therefore, each group has unequal resources to manage the disease depending on their specific local context. The simulation is followed by a discussion about equity in disease management. To make this activity broadly applicable, the lesson plan has been written in four languages (English, Portuguese, Spanish, and Mandarin) according to a diversity of corresponding locations (Texas-USA, Brazil, Spain, and China), which are included in the supplementary materials. Incorporating this lesson into curricula across multiple disciplines is an important step for developing future entomologists, parasitologists, epidemiologists, and other One Health professionals to address disparities in VBD control and help drive the field forward equitably.</div></div>","PeriodicalId":19577,"journal":{"name":"One Health","volume":"20 ","pages":"Article 101019"},"PeriodicalIF":4.1,"publicationDate":"2025-03-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143714872","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Detection and characterization of Langya virus in Crocidura lasiura (the Ussuri white-toothed shrew), Republic of Korea","authors":"Augustine Natasha ,&nbsp;Sarah E. Pye ,&nbsp;Kyungmin Park ,&nbsp;Shivani Rajoriya ,&nbsp;Intae Yang ,&nbsp;Jieun Park ,&nbsp;Haryo Seno Pangestu ,&nbsp;Jongwoo Kim ,&nbsp;Yeonsu Oh ,&nbsp;Carolina B. López ,&nbsp;Jin-Won Song ,&nbsp;Won-Keun Kim","doi":"10.1016/j.onehlt.2025.101017","DOIUrl":"10.1016/j.onehlt.2025.101017","url":null,"abstract":"<div><div>Langya virus (LayV) is the only documented zoonotic agent within the shrew borne <em>Parahenipavirus</em> genus. Other <em>Parahenipavirus</em> species, including Gamak virus and Daeryeong virus, have been discovered in the Republic of Korea, highlighting the prevalence of this genus in the region. We retrospectively analyzed metagenomic next-generation sequencing of two <em>Crocidura lasiura</em> (the Ussuri white-toothed shrew) kidney samples from 2017, followed by paramyxovirus screening of 24 kidney samples from the same species collected in 2023. The LayV positivity rate was 12.5 % (3 of 24). Amplicon-based sequencing was subsequently developed to obtain the complete viral sequences. Five complete genomes of Langya virus Korea (LayV KOR) were identified: two from 2017 samples and three from 2023 samples. LayV KOR exhibited approximately 80 % and 95.5 % homology at the nucleotide and amino acid levels, respectively. Phylogenetic analysis underscored the close relationship between LayV KOR and LayV from China. This study represents the first detection of LayV complete sequences in shrews outside of China.</div></div>","PeriodicalId":19577,"journal":{"name":"One Health","volume":"20 ","pages":"Article 101017"},"PeriodicalIF":4.1,"publicationDate":"2025-03-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143685946","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"DNA metabarcoding analysis revealed a silent prevalence of environmental pathogenic Leptospira in urban area of Okinawa Island, Japan","authors":"Yukuto Sato ,&nbsp;Yuiko Hiyajo ,&nbsp;Taisei Tengan ,&nbsp;Tsurua Yoshida ,&nbsp;Yoichiro Uchima ,&nbsp;Michinari Tokeshi ,&nbsp;Kaori Tsurui-Sato ,&nbsp;Claudia Toma","doi":"10.1016/j.onehlt.2025.101016","DOIUrl":"10.1016/j.onehlt.2025.101016","url":null,"abstract":"<div><h3>Objective</h3><div>Human activities, such as agriculture, environmental manipulation, and city development, have impacted the distribution of flora, fauna, and microbes (including potential human pathogens) at the global level. This study focused on the bacterial genus <em>Leptospira</em>, an organism causing leptospirosis that is prevalent in tropical and subtropical regions. We hypothesized that although only a few cases of leptospirosis have been reported in the urban region of main island of Okinawa Prefecture (Okinawa Island, OKI), Japan, <em>Leptospira</em> is present in these regions.</div></div><div><h3>Methods</h3><div>Thirty-four samples were collected from rivers in urban OKI and rural Ishigaki Island (ISG) and analyzed to determine the distribution of <em>Leptospira</em> and mammals using environmental DNA (eDNA) metabarcoding. High-throughput sequencing analysis was performed to sequence the polymerase chain reaction products of partial leptospiral 16S rRNA and vertebrate mitochondrial 12S rRNA genes from 16 and 18 river samples of OKI and ISG, respectively, including the waters collected from <em>Leptospira</em>-endemic areas in ISG.</div></div><div><h3>Results</h3><div><em>Leptospira noguchii</em> and <em>L. interrogans</em>-related, two <em>Leptospira</em> species of the P1+ clade that are pathogenic to humans and mammals, were repeatedly detected in OKI and ISG, supporting our hypothesis. The sequence numbers of the five <em>Leptospira</em> species of P1– and P2 clades showed significant correlations with those of cattle (<em>Bos taurus</em>) in OKI; however, the potential host animals for P1+ species remain unclear. The total number of leptospiral sequences obtained from the ISG samples was correlated with the distance from the mountainous woodlands.</div></div><div><h3>Conclusion</h3><div>The pathogenic P1+ <em>Leptospira</em> was distributed in urban OKI, in addition to rural ISG. The factors correlated with leptospiral detection, that is, cattle eDNAs and the distance from mountainous forests in OKI and ISG, respectively, suggest the silent prevalence of <em>Leptospira</em> in urban and developing regions related to human activities. The findings of the present study provide insights into public health in cities with respect to climate change and possible flood damage.</div></div>","PeriodicalId":19577,"journal":{"name":"One Health","volume":"20 ","pages":"Article 101016"},"PeriodicalIF":4.1,"publicationDate":"2025-03-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143705125","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Molecular evidence of host range expansion of Enterocytozoon bieneusi in captive wildlife at Beijing Zoo, China","authors":"Zhiyang Pei ,&nbsp;Bowen Zhang ,&nbsp;Yongqiang He ,&nbsp;Qianming Zhao ,&nbsp;Fuchang Yu ,&nbsp;Ting Jia ,&nbsp;Zhenjie Zhang ,&nbsp;Meng Qi","doi":"10.1016/j.onehlt.2025.101011","DOIUrl":"10.1016/j.onehlt.2025.101011","url":null,"abstract":"<div><div><em>Enterocytozoon bieneusi</em> has become a significant public health concern due to its ability to infect both humans and animals on a global scale. However, there is a paucity of epidemiological data regarding this pathogen in captive wildlife. In this study, 445 fecal samples were collected from captive animals at Beijing Zoo and analyzed using PCR targeting the internal transcribed spacer (ITS) of the nuclear ribosomal DNA of <em>E. bieneusi</em>. The overall prevalence was determined to be 7.0 % (31/445), with infection rates of 7.1 % (23/322) in mammals and 6.5 % (8/123) in Aves. Genotypic characterization 12 distinct genotypes, including 11 known genotypes belonging to Groups 1 (EbpA, PigEBITS7, D, HND-1, SC02, CM16, MJ13), 2 (BEB6), 7 (CM 4), and 13 (CHB1, CHK1), along with one novel genotype (BJZ-1) in Group 1. The detection of zoonotic genotypes from Groups 1 and 2 underscores the potential for cross-species transmission between captive wildlife and humans. Notably, this study presents the first molecular identification of <em>E. bieneusi</em> in caracals, polar bears, giant anteaters, and grey herons, thereby significantly expanding the known host range of this pathogen. These findings emphasise the necessity of implementing One Health surveillance strategies to monitor and mitigate zoonotic risks at the human-animal interface.</div></div>","PeriodicalId":19577,"journal":{"name":"One Health","volume":"20 ","pages":"Article 101011"},"PeriodicalIF":4.1,"publicationDate":"2025-03-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143654540","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}