One Health最新文献

{"title":"Asymmetric association between meteorological factors and human infections with hemorrhagic fever with renal syndrome: A 16-year ecological trend study in Shaanxi, China","authors":"Chenlu Xue ,&nbsp;Bingjie Zhang ,&nbsp;Yanyan Li ,&nbsp;Xinxiao Li ,&nbsp;Chunjie Xu ,&nbsp;Yongbin Wang","doi":"10.1016/j.onehlt.2024.100895","DOIUrl":"10.1016/j.onehlt.2024.100895","url":null,"abstract":"<div><h3>Objective</h3><p>Hemorrhagic fever with renal syndrome (HFRS) continues to pose a significant threat to global health. This study aimed to investigate both the long- and short-term asymmetric impacts of variations in meteorological variables on HFRS.</p></div><div><h3>Methods</h3><p>The reported monthly HFRS incidence data from Shaanxi between 2004 and 2019, along with corresponding meteorological data, were collected to conduct an ecological trend analysis. Subsequently, the autoregressive distributed lag (ARDL) and nonlinear ARDL (NARDL) models were used to examine the long- and short-term asymmetric effects of climate variables on HFRS incidence.</p></div><div><h3>Results</h3><p>Overall, a reduction in HFRS incidence was observed in Shaanxi from 2004 to 2019, with an average annual percentage change of −0.498 % (95 %<em>CI</em> -13.247 % to 12.602 %). HFRS incidence peaked in December and reached its lowest point in March each year. A 1 mm increase in aggregate precipitation (AP) was associated with a 4.3 % rise in HFRS incidence, while a 1 mm decrease contributed to a 3.7 % increase, indicating a long-term asymmetric impact (Wald long-term asymmetry test [WLT] = 9.072, <em>P</em> = 0.003). In the short term, a 1 % decrease in mean relative humidity (MRH) led to a 5.7 % decline in HFRS incidence (Wald short-term asymmetry test [WSR] = 5.978, <em>P</em> = 0.015). Additionally, changes in meteorological variables showed varied effects: ΔMWV(+) at a 1-month lag had a significant positive short-term effect on HFRS; ΔMRH(+) at a 3-month lag, ΔAP(+) at a 2-month lag, ΔAP(−) at a 1-month lag, ΔASH(+) at a 1-month lag, and ΔASH(−) at a 3-month lag all exhibited strong negative short-term impacts on HFRS incidence.</p></div><div><h3>Conclusions</h3><p>Weather variability plays a significant role in influencing HFRS incidence, with both long- and short-term asymmetric and/or symmetric effects. Utilizing the NARDL model through a One Health lens offers promising opportunities for enhancing HFRS control measures.</p></div>","PeriodicalId":19577,"journal":{"name":"One Health","volume":"19 ","pages":"Article 100895"},"PeriodicalIF":4.1,"publicationDate":"2024-09-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2352771424002210/pdfft?md5=9d9590707b153e6e29a8adf4859e0df1&pid=1-s2.0-S2352771424002210-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142242637","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Waste management and disease spread potential: A case study of SARS-CoV-2 in garbage dumping sites in Bangkok and its vicinity","authors":"Anuwat Wiratsudakul ,&nbsp;Ladawan Sariya ,&nbsp;Weena Paungpin ,&nbsp;Sarin Suwanpakdee ,&nbsp;Tatiyanuch Chamsai ,&nbsp;Siriporn Tangsudjai ,&nbsp;Benjaporn Bhusri ,&nbsp;Peerawat Wongluechai ,&nbsp;Kanittha Tonchiangsai ,&nbsp;Walasinee Sakcamduang ,&nbsp;Witthawat Wiriyarat ,&nbsp;Nareerat Sangkachai","doi":"10.1016/j.onehlt.2024.100894","DOIUrl":"10.1016/j.onehlt.2024.100894","url":null,"abstract":"<div><p>During the coronavirus disease 2019 (COVID-19) pandemic, hospitals and households have used personal protective equipment (PPE), such as masks and gloves. Some of these potentially infectious materials were discarded with other household wastes in garbage dumping sites. Thus, this study aimed to detect the presence of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) in contaminated wastes, environments, and mammals scavenging around these sites. From September to October 2022, we visited three garbage dumping sites located in Bangkok, Nakhon Pathom, and Nonthaburi provinces of Thailand. Oral, nasal, rectal swabs, and blood samples were collected from small mammals, stray dogs, and cats. Masks, gloves, soil, and water samples from the sites were additionally collected. Of the 582 samples collected from 238 animals, none tested positive for SARS-CoV-2 in the virus isolation, real-time reverse-transcription polymerase chain reaction, and neutralizing antibody detection. However, one sample (1.18 %; 1/85) from a rat (<em>Rattus</em> spp.) captured in Nonthaburi was serologically positive in the indirect enzyme-linked immunosorbent assay. The surveillance of coronaviruses in rats is strongly encouraged because rats may harbor different zoonotic pathogens, including unknown potentially zoonotic coronaviruses. Moreover, two face mask samples (4.65 %; 2/43) collected from the dumping site in Nakhon Pathom tested positive for SARS-CoV-2 by real-time RT-PCR. To reduce environmental contamination, detecting the SARS-CoV-2 viral genome in contaminated face masks highlights the critical need for proper waste management in households and communities in Thailand. Thus, to minimize exposure and prevent onward transmission, waste management personnel, including garbage dump staff and waste pickers, should be equipped with appropriate PPE and receive regular training on safe handling and disposal.</p></div>","PeriodicalId":19577,"journal":{"name":"One Health","volume":"19 ","pages":"Article 100894"},"PeriodicalIF":4.1,"publicationDate":"2024-09-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2352771424002209/pdfft?md5=b03cfa666c1331b7ec4816dddbff21b0&pid=1-s2.0-S2352771424002209-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142267351","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Threatened by many complex food security problems - agriculture, academic, and government professionals seek new one health research partnerships","authors":"Gregory C. Gray ,&nbsp;Thang Nguyen-Tien","doi":"10.1016/j.onehlt.2024.100890","DOIUrl":"10.1016/j.onehlt.2024.100890","url":null,"abstract":"<div><p>With modern international trade and the rapid movement of people, animals, and food products, today's risks to food security are increasing worldwide. It is clear that human health, animal health, environmental health, and agriculture are inextricable linked. Food security problems in one sector often adversely impact multiple other sectors. Food security threats are often complex with many factors influencing the emergence of new threats. As multiple US governmental agencies share responsibilities for food security threat mitigation, we need to find new ways for the numerous food security scientific disciplines and agencies to forge new effective research collaborations in meeting these threats. This special issue of the journal <em>One Health</em> seeks to give examples of such research. It represents a collection of scientific reports from oral and poster presentations delivered at the April 21–23, 2024, “International Symposium on One Health Research: Improving Food Security and Resilience” in Galveston, Texas.</p></div>","PeriodicalId":19577,"journal":{"name":"One Health","volume":"19 ","pages":"Article 100890"},"PeriodicalIF":4.1,"publicationDate":"2024-09-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2352771424002167/pdfft?md5=922b4ef528f0a238c370d46ee06e89e9&pid=1-s2.0-S2352771424002167-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142173118","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The genomic diversity and antimicrobial resistance of Non-typhoidal Salmonella in humans and food animals in Northern India","authors":"Jaspreet Mahindroo ,&nbsp;Duy Pham Thanh ,&nbsp;Harpreet Kaur ,&nbsp;To Nguyen Thi Nguyen ,&nbsp;Trang Hoang Thu Nguyen ,&nbsp;Megan E. Carey ,&nbsp;Ritu Verma ,&nbsp;Balvinder Mohan ,&nbsp;Siddhartha Thakur ,&nbsp;Stephen Baker ,&nbsp;Neelam Taneja","doi":"10.1016/j.onehlt.2024.100892","DOIUrl":"10.1016/j.onehlt.2024.100892","url":null,"abstract":"<div><h3>Introduction</h3><p>Non-typhoidal <em>Salmonella</em> (NTS) serovars are the leading global cause of gastroenteritis and have established reservoirs in food animals.</p></div><div><h3>Gap statement</h3><p>Due to a lack of surveillance, there is limited information on the distribution of NTS serovars in India.</p></div><div><h3>Aim</h3><p>Here, we investigated the epidemiology, sequence types, serovar distribution, phylogenetic relatedness, and antimicrobial resistance patterns of NTS in humans and animals across a large geographic area in Northern India.</p></div><div><h3>Methodology</h3><p>We collected stool samples from patients with diarrhea who presented to 14 laboratories in Chandigarh and from five states in India (Punjab, Haryana, Uttarakhand, Himachal Pradesh, and Rajasthan). We sequenced the genomes and analyzed 117 NTS organisms isolated from humans and animals. Minimum inhibitory concentrations (MICs) were estimated using a Vitek2 system.</p></div><div><h3>Results</h3><p>The prevalence of NTS in participants presenting to our study with diarrhea was 1.28 %, affecting all age groups. All NTS caused moderate to severe diarrhea. We found a high diversity of serovars with considerable serovar and sequence types (STs) overlap and phylogenetic closeness between isolates from human infections and food animals. We report serovars such as <em>S.</em> Agona, <em>S.</em> Bareilly, <em>S.</em> Kentucky, <em>S.</em> Saintpaul, and <em>S.</em> Virchow, causing human infections from north India for the first time. Among the different food-producing animals, pigs appeared to be a key source of human infections. Twenty-eight percent (28 %) of the NTS isolates were multi-drug resistant (MDR), and human isolates showed a higher proportion of resistance. A higher level of contamination of meat samples in our study (8.4 %) potentially suggests a close association of NTS serovars with the food chain and high transmission risk in north India.</p></div><div><h3>Conclusions</h3><p>This study provides information on AMR genes and plasmid replicons associated with different serovars and highlights the role of food animals in AMR dissemination in our region.</p></div>","PeriodicalId":19577,"journal":{"name":"One Health","volume":"19 ","pages":"Article 100892"},"PeriodicalIF":4.1,"publicationDate":"2024-09-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2352771424002180/pdfft?md5=b9f5651cd23e2607064dcde664e46f98&pid=1-s2.0-S2352771424002180-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142267284","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Epidemiology and evolution of human-origin H10N5 influenza virus","authors":"Zhaoxia Yuan ,&nbsp;Jiahao Zhang ,&nbsp;Danli Jiang ,&nbsp;Guangyu Huang ,&nbsp;Wenbao Qi","doi":"10.1016/j.onehlt.2024.100893","DOIUrl":"10.1016/j.onehlt.2024.100893","url":null,"abstract":"<div><p>H10 subtype avian influenza viruses were endemic in wild and domestic avian species worldwide. Strikingly, it frequently crossed the species barrier to infect mammalian hosts. Human infection with H10N3 and H10N8 were reported previously. Recently, a 63-year-old woman from Anhui province of China who died from a mixed infection of H3N2 and H10N5 influenza viruses, which have drawn widespread public health attention. Here, we perform the evolutionary dynamics of H10N5 influenza viruses of bird- and human-origin worldwide, and found that wild bird-origin H10N5 influenza viruses from China did not cluster together with human-origin H10N5 influenza viruses, while grouped together with LPAIV gene pools circulating in wild birds that derived from other Eurasian countries. Human-derived H10N5 virus is a novel reassortant, which frequently reassorted with wild bird-derived influenza viruses, and in turn, spillover into humans. Collectively, our results suggested that H10 subtype influenza viruses continuously pose threat to public health.</p></div>","PeriodicalId":19577,"journal":{"name":"One Health","volume":"19 ","pages":"Article 100893"},"PeriodicalIF":4.1,"publicationDate":"2024-09-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2352771424002192/pdfft?md5=cb4ba74b582f8e9ea11f0d51f9fd94dd&pid=1-s2.0-S2352771424002192-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142267354","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Catastrophic floods and antimicrobial resistance: Interconnected threats with wide-ranging impacts","authors":"João Pedro Rueda Furlan ,&nbsp;Fábio Parra Sellera ,&nbsp;Nilton Lincopan ,&nbsp;Daniela Debone ,&nbsp;Simone Georges El Khouri Miraglia ,&nbsp;Ronan Adler Tavella","doi":"10.1016/j.onehlt.2024.100891","DOIUrl":"10.1016/j.onehlt.2024.100891","url":null,"abstract":"","PeriodicalId":19577,"journal":{"name":"One Health","volume":"19 ","pages":"Article 100891"},"PeriodicalIF":4.1,"publicationDate":"2024-09-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2352771424002179/pdfft?md5=3d19433eecc2846d0c4bfd06ea8e7c8d&pid=1-s2.0-S2352771424002179-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142161834","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Application of compound poisson model to estimate underreported risk of non-communicable diseases in underdeveloped areas","authors":"Hongli Wan ,&nbsp;Wenhui Zhu ,&nbsp;Jingmin Yan ,&nbsp;Xinyue Han ,&nbsp;Jie Yu ,&nbsp;Qiang Liao ,&nbsp;Tao Zhang","doi":"10.1016/j.onehlt.2024.100889","DOIUrl":"10.1016/j.onehlt.2024.100889","url":null,"abstract":"<div><h3>Background</h3><p>Hypertension and diabetes are major components of non-communicable diseases (NCDs), with a substantial number of patients residing in underdeveloped areas. Limited medical resources in these areas often results in underreporting of disease prevalence, masking the true extent of diseases. Taking the underdeveloped Liangshan Yi Autonomous Prefecture in China as an example, this study aimed to correct the underreported prevalence of hypertension and type 2 diabetes so as to provide inspiration for the allocation of medical resources in such areas.</p></div><div><h3>Methods</h3><p>Assuming the true number of patients in each area follows a Poisson distribution, we applied a Compound Poisson Model based on Clustering of Data Quality (CPM-CDQ) to estimate the potential true prevalence of hypertension and diabetes, as well as the registration rate of existing patients. Specifically, a hierarchical clustering approach was utilized to group the counties based on the data quality, and then the registration rate of the cluster with the best data quality was used as a priori information for the model. The model parameters were estimated by the maximum likelihood method. Sensitivity analyses were performed to test the robustness of the model.</p></div><div><h3>Results</h3><p>The estimated prevalence of hypertension in the entire Liangshan Prefecture from 2018 to 2020 ranged from 24.59 % to 25.28 %, and for diabetes, it ranged from 4.95 % to 8.42 %. The registration rates for hypertension and diabetes were 14.10 % to 24.59 % and 15.98 % to 29.12 %, respectively. Additionally, the accuracy of clustering the counties with the best data quality had a significant impact on the performance of the model.</p></div><div><h3>Conclusion</h3><p>Liangshan Prefecture is experiencing a significant high prevalence of hypertension and diabetes, accompanied by a concerningly low registration rate. The CPM-CDQ proved useful for assessing underreporting risks and facilitating targeted interventions for NCDs control and prevention, particularly in underdeveloped areas.</p></div>","PeriodicalId":19577,"journal":{"name":"One Health","volume":"19 ","pages":"Article 100889"},"PeriodicalIF":4.1,"publicationDate":"2024-09-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2352771424002155/pdfft?md5=ae450be66848a4babd3f97828e590b79&pid=1-s2.0-S2352771424002155-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142228488","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Unveiling spatial patterns of West Nile virus emergence in northern Greece, 2010–2023","authors":"Anastasia Angelou ,&nbsp;Lea Schuh ,&nbsp;Nikolaos I. Stilianakis ,&nbsp;Spiros Mourelatos ,&nbsp;Ioannis Kioutsioukis","doi":"10.1016/j.onehlt.2024.100888","DOIUrl":"10.1016/j.onehlt.2024.100888","url":null,"abstract":"<div><p>The Region of Central Macedonia (RCM) in Northern Greece recorded the highest number of human West Nile virus (WNV) infections in Greece, despite considerable local mosquito control actions. We examined spatial patterns and associations of mosquito levels, infected mosquito levels, and WNV human cases (WNVhc) across the municipalities of this region over the period 2010–2023 and linked it with climatic characteristics. We combined novel entomological and available epidemiological and climate data for the RCM, aggregated at the municipality level and used Local and Global Moran's I index to assess spatial associations of mosquito levels, infected mosquito levels, and WNVhc. We identified areas with strong interdependencies between adjacent municipalities in the Western part of the region. Furthermore, we employed a Generalized Linear Mixed Model to first, identify the factors driving the observed levels of mosquitoes, infected mosquitoes and WNVhc and second, estimate the influence of climatic features on the observed levels. This modeling approach indicates a strong dependence of the mosquito levels on the temperatures in winter and spring and the total precipitation in early spring, while virus circulation relies on the temperatures of late spring and summer. Our findings highlight the significant influence of climatic factors on mosquito populations (∼60 % explained variance) and the incidence of WNV human cases (∼40 % explained variance), while the unexplained ∼40 % of the variance suggests that targeted interventions and enhanced surveillance in identified hot-spots can enhance public health response.</p></div>","PeriodicalId":19577,"journal":{"name":"One Health","volume":"19 ","pages":"Article 100888"},"PeriodicalIF":4.1,"publicationDate":"2024-09-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2352771424002143/pdfft?md5=4c50a78df74334f1a8e87cd7b5cd4e5d&pid=1-s2.0-S2352771424002143-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142152008","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Emergence of multidrug-resistant Providencia rettgeri clone in food-producing animals: A public health threat","authors":"Tiago Barcelos Valiatti ,&nbsp;Fernanda Fernandes Santos ,&nbsp;Francisco Ozório Bessa-Neto ,&nbsp;Ruanita Veiga ,&nbsp;Simone Simionatto ,&nbsp;Gleyce Hellen de Almeida Souza ,&nbsp;Márcia Soares Mattos Vaz ,&nbsp;Antônio Carlos Campos Pignatari ,&nbsp;Rodrigo Cayô ,&nbsp;Ana Cristina Gales ,&nbsp;Guarani Network","doi":"10.1016/j.onehlt.2024.100887","DOIUrl":"10.1016/j.onehlt.2024.100887","url":null,"abstract":"<div><p>The occurrence of carbapenemases encoding genes in <em>Providencia rettgeri</em> is a critical public health concern since this species has intrinsic resistance to several antimicrobials, including polymyxins. The identification of this multidrug-resistant (MDR) pathogen outside the hospital setting has become increasingly frequent, and raises an alert for the global health agencies, as they indicate a possible spread of such pathogens. Herein, we described three MDR <em>P. rettgeri</em> isolates carrying a diversity of antimicrobial resistance genes (ARGs) isolated from stool samples of swine and bovine in Brazil. Molecular analysis revealed that all isolates belonged to the same clone. The whole genome sequencing (WGS) of a representative isolate (PVR-188) was performed by MiSeq Illumina® platform, while the assembling and annotation was achieved using SPAdes and Prooka, respectively. The WGS analyses indicated the presence of ARGs that confer resistance to β-lactams (<em>bla</em>_NDM-1, <em>bla</em>_CTX-M-2), quinolones (<em>qnrD1</em>), aminoglycosides (<em>aadA</em>2, <em>aadA1</em>, <em>aph(3′)-Via</em>), phenicol (<em>catB2</em>), sulfonamides (<em>sul1</em>, <em>sul2</em>), and trimethoprim (<em>dfrA12</em>, <em>dfrA1</em>). The presence of three plasmid replicons (Col3M, IncQ1, and IncT) was detected, but no phage sequences were found. The phylogenetic analyses confirmed the genomic relationship of the PVR-188 with <em>P. rettgeri</em> isolates recovered from animals and humans in the USA and Malaysia. In conclusion, we report the occurrence of MDR <em>P. rettgeri</em> clone colonizing the gut microbiota of food-producing animals in Brazil, revealing the spread of this pathogen beyond hospital boundaries.</p></div>","PeriodicalId":19577,"journal":{"name":"One Health","volume":"19 ","pages":"Article 100887"},"PeriodicalIF":4.1,"publicationDate":"2024-09-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2352771424002131/pdfft?md5=9370bc9f90f29b4a6f158b046957bbe0&pid=1-s2.0-S2352771424002131-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142242638","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Multi-drug resistant (MDR) and extended-spectrum β-lactamase (ESBL) producing Escherichia coli isolated from slaughtered pigs and slaughterhouse workers in Yaoundé, Cameroon","authors":"Moise Matakone ,&nbsp;Raspail Carrel Founou ,&nbsp;Luria Leslie Founou ,&nbsp;Brice Davy Dimani ,&nbsp;Patrice Landry Koudoum ,&nbsp;Marie Christine Fonkoua ,&nbsp;Yap Boum-II ,&nbsp;Hortense Gonsu ,&nbsp;Michel Noubom","doi":"10.1016/j.onehlt.2024.100885","DOIUrl":"10.1016/j.onehlt.2024.100885","url":null,"abstract":"<div><p>Antimicrobial resistance (AMR) in the food chain remains a global public health concern for both humans and animals. This study aimed to determine the prevalence, resistance profiles, and clonal relatedness of multidrug-resistant (MDR) and extended-spectrum β-lactamases- producing <em>Escherichia coli</em> (ESBL-<em>Ec</em>) isolated from slaughtered pigs and slaughterhouse workers in Yaoundé, Cameroon.</p><p>A cross-sectional study was conducted over four months, from February to May 2023 in two selected pig's slaughterhouse markets in Yaoundé. Rectal swabs were collected from 375 pigs at four time points and pooled per three according to gender, origin, and abattoirs leading to 125 pooled samples. Seven faecal samples from 60 contacted exposed workers were collected. Samples were cultured on CHROMagar™ ESBL medium, dark pink to reddish colonies were considered <em>E. coli</em>. Resistance genes including <em>bla</em>_CTX-M, <em>bla</em>_SHV and <em>bla</em>_TEM were detected using the polymerase chain reaction (PCR) while ERIC-PCR was used to assess the genetic relatedness between isolates.</p><p>The prevalence of ESBL-<em>Ec</em> was elevated among exposed workers (71.4 %; <em>n</em> = 5/7) and pigs (70.4 %; <em>n</em> = 88/125). Overall, ESBL-<em>Ec</em> exhibited high resistance to cefuroxime (100 %, <em>n</em> = 105/105), cefotaxime (100 %, n = 105/105), amoxicillin-clavulanic acid (98.1 %, <em>n</em> = 103/105), cefixime (92.4 %, <em>n</em> = 97/105), tetracycline (86.7 %, <em>n</em> = 91/105) and sulfamethoxazole-trimethoprim (81.9 %, <em>n</em> = 86/105). However, these isolates showed good susceptibility to gentamicin (3.8 %, <em>n</em> = 4/105), chloramphenicol (8.6 %, n = 9/105), and fosfomycin (14.3 %, <em>n</em> = 15/105)<em>.</em> All human isolates and 75.8 % (<em>n</em> = 75/99) of pig isolates were multi-drug resistant. The <em>bla</em>_CTX-M was the most prevalent resistance gene among exposed workers (100 %, <em>n</em> = 6/6) and pigs (80.8 %, <em>n</em> = 80/99) followed by <em>bla</em>_TEM (33.3 % each). High clonal relatedness of ESBL-<em>Ec</em> strains was observed among pig and human isolates across slaughterhouses.</p><p>This study showed that the gastrointestinal tract of pigs might be an important reservoir of MDR and ESBL-<em>Ec</em> in Yaoundé, Cameroon and these resistant bacteria might be circulating between sources, especially humans. Heightening awareness on appropriate antibiotic use in humans and animals as well as implementing stringent biosecurity and food safety measures are imperative to prevent the emergence and spread of AMR in the country.</p></div>","PeriodicalId":19577,"journal":{"name":"One Health","volume":"19 ","pages":"Article 100885"},"PeriodicalIF":4.1,"publicationDate":"2024-08-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2352771424002118/pdfft?md5=8973df5d1680e663d9738be6ad6989c3&pid=1-s2.0-S2352771424002118-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142098466","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}