One Health最新文献

{"title":"Guidelines for addressing disease risks in wildlife trade","authors":"William B. Karesh ,&nbsp;Tiggy Grillo ,&nbsp;Catherine Machalaba ,&nbsp;Helen Roberts ,&nbsp;François Diaz ,&nbsp;Sophie Muset ,&nbsp;Keith Hamilton","doi":"10.1016/j.onehlt.2025.100998","DOIUrl":"10.1016/j.onehlt.2025.100998","url":null,"abstract":"<div><div>Wildlife trade for commercial and subsistence purposes has a long history and economic, cultural and religious value, but also presents potential trade-offs, including for human and animal (domestic and wild) health, animal welfare, species conservation, and ecosystems. Understanding and balancing the many dimensions of trade requires a holistic approach. To assist in decision making, the World Organisation for Animal Health (WOAH) undertook a process to develop draft guidelines, convening a multi-sectoral ad hoc group, reinforced with expertise from a WOAH Collaborating Centre and a peer review process. The draft guidelines were piloted through a workshop series in Bangkok, Thailand, informing their refinement and finalization. The Guidelines for Addressing Disease Risks in Wildlife Trade, published in May 2024, present a high-level framework to assess risk and identify risk-management strategies for wildlife trade. The Guidelines are structured by four main sections, stakeholder engagement and system mapping; risk analysis (the stages of hazard identification, risk assessment, risk management, and risk communication); monitoring and evaluation; and tools and guidance. Rather than being prescriptive, the Guidelines can be a basis for advancing context-specific approaches to addressing disease risk in the wildlife trade using a One Health lens, supported by workshops and multi-sectoral collaboration to strengthen risk analysis competencies and implementation. This paper describes the process of developing the Guidelines and summarizes their content and anticipated use.</div></div>","PeriodicalId":19577,"journal":{"name":"One Health","volume":"20 ","pages":"Article 100998"},"PeriodicalIF":4.1,"publicationDate":"2025-02-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143550667","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Metabarcoding analysis of the microbiota in flocks naturally infected by Coxiella burnetii: First description of the global microbiota in domestic small ruminants","authors":"R. Toledo-Perona ,&nbsp;Á. Gómez-Martín ,&nbsp;A. Contreras ,&nbsp;M. Toquet ,&nbsp;J.J. Quereda ,&nbsp;A. Esnal ,&nbsp;P. González-Torres ,&nbsp;J. Gomis","doi":"10.1016/j.onehlt.2025.100996","DOIUrl":"10.1016/j.onehlt.2025.100996","url":null,"abstract":"<div><div>This study investigates Q fever in sheep and goats, key reservoirs for human infection, by metabarcoding and comparing it with q-PCR and serology. Samples from 26 small ruminants (aborted and normal-delivery) and six males across three Q fever-affected herds were analyzed. In sheep herds, seropositivity was 50 and 80 % respectively, with <em>Coxiella (C.) burnetii</em> shedding detected vaginally in the second herd. In goats, 100 % seropositivity and 90 % <em>C. burnetii</em> detection were observed, with nasal and vaginal samples showing the highest detection rates. Metabarcoding revealed significant differences in alpha diversity, with greater richness in blood and evenness in milk from normal-delivery sheep and higher evenness in faeces from aborted sheep. Beta diversity showed distinct vaginal microbiota in normal-delivery females compared to aborted ones. Firmicutes was the most abundant phylum observed. Dominant genera included: <em>Moraxella</em> (nasal), <em>Mycoplasma</em> (blood), <em>Streptococcus</em> (milk), <em>Ureaplasma</em> (vaginal and preputial), <em>Rikenellaceae RC9 gut group</em> (faeces). Significant differences in bacterial composition, including infertility-linked vaginal pathogens, were found across female groups in all herds in the anatomical locations studied, revealing new species and tropisms. Moreover, taxonomic analysis identified <em>C. burnetii</em> in vaginal, milk and environmental samples. This first report of <em>C. burnetii</em> in the caprine nasal cavity suggests an underestimated tropism that may improve Q fever diagnosis. These findings underscore the need for herd-wide Q fever control measures, including males and normal-delivery females. Our findings contribute to new insights into the pathogen's impact on small ruminant microbiota and a novel approach to studying infectious diseases in this sector.</div></div>","PeriodicalId":19577,"journal":{"name":"One Health","volume":"20 ","pages":"Article 100996"},"PeriodicalIF":4.1,"publicationDate":"2025-02-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143480511","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Structure, conservation and health implications of urban wild meat value chains: A case study of Lagos, Nigeria","authors":"Samuel N. Akpan ,&nbsp;Pim van Hooft ,&nbsp;Anise N. Happi ,&nbsp;Ralph Buij ,&nbsp;Frank van Langevelde ,&nbsp;Elizabeth A.J. Cook ,&nbsp;James M. Hassell ,&nbsp;Dawn M. Zimmerman ,&nbsp;Sherrill P. Masudi ,&nbsp;Christian T. Happi ,&nbsp;Lian F. Thomas","doi":"10.1016/j.onehlt.2025.100992","DOIUrl":"10.1016/j.onehlt.2025.100992","url":null,"abstract":"<div><div>Urban wild meat value chains represent a vital source of protein and livelihood intertwined with culture and complex market systems. Against the backdrop of escalating zoonotic disease concerns linked to wild meat, this research aimed to unravel the structure, governance, species composition and actor characteristics of the wild meat value chain in Lagos, Africa's most populated city. We employed a cross-sectional study design incorporating qualitative and quantitative approaches. Interviews (<em>n</em> = 22) were conducted and structured questionnaires were administered to participants (<em>n</em> = 257) across 15 sites in Lagos. Participants' activities were also observed, and field notes were taken. Descriptive statistics and inductive thematic approach were used for data analysis. Findings revealed five main value chain nodes: hunter, processor, wholesale, retail and consumer; and five major geographical areas from which wild meat flowed into the city. Governance structures showed a blend of informal and formal mechanisms sustained through trust, cultural beliefs and informal regulations. The main motivation was income (88.1 %), and period of optimum activity was at night times (53 %) during the dry season (≥ 62 %). Women (62.2 %) dominated the value chain, prevalent at the wholesaler (61.7 %), processor (89.7 %) and retailer (83 %) nodes. Hunters (40 %) were able to supply wild meat directly to consumers, and the large-scale retailers (26 %) were responsible for exportation of wild meat. A total of 35 species were traded in the value chain, and the most commonly traded species were: grasscutters, duikers, porcupines, and giant-pouched rats (≥90.7 % participants). Pottos, buffalos, tortoises and house snakes were the least traded (≤ 7.0 % participants). This study provides an understanding of Lagos wildlife trade from a value chain perspective, paving the way for interventions to address wildlife conservation challenges and spillover risks. Species traded in the value chain have been globally reported as reservoirs of zoonotic pathogens, representing a source of zoonotic spillover risks to actors. Also, the ability of hunters to supply wild meat directly to consumers signals an increase in the speed of zoonotic pathogen spread, and portends a greater risk for public health. Formal governance should be integrated into the value chain to aid effective monitoring and regulation. Conservation and public health interventions should be node-specific and gender-sensitive, targeting the dry seasons when actors' activities peak, and risks of human-wild meat contact are greater. There is need for the re-assessment of the conservation statuses of West Africa's wildlife species to reflect current realities foisted by wild meat trade.</div></div>","PeriodicalId":19577,"journal":{"name":"One Health","volume":"20 ","pages":"Article 100992"},"PeriodicalIF":4.1,"publicationDate":"2025-02-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143429723","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Systematic review on evaluation tools applicable to One Health surveillance systems: A call for adapted methodology","authors":"Sarah Mediouni ,&nbsp;Claire Ndione ,&nbsp;E. Jane Parmley ,&nbsp;Thomas G. Poder ,&nbsp;Hélène Carabin ,&nbsp;Cécile Aenishaenslin","doi":"10.1016/j.onehlt.2025.100995","DOIUrl":"10.1016/j.onehlt.2025.100995","url":null,"abstract":"<div><div>Developing and implementing effective surveillance programs for infectious diseases (ID) and antimicrobial resistance (AMR) requires the integration of information across relevant disciplines and sectors. Yet, establishing and sustaining collaboration at each step of the surveillance process, and modalities to translate integrated surveillance results into actions, are not well understood. This systematic review was designed to map and explore peer-reviewed tools that were either designed or used for evaluation of integrated surveillance systems for ID or AMR, and to identify the limitations of these tools and remaining methodological or knowledge gaps. A systematic search was conducted using keywords related to: “Evaluation”, “Surveillance” and “One Health” in four databases (Medline, Embase, Web of Science and CAB abstract) up to the 28th of October 2022. Articles were selected if they presented an evaluation tool for integrated surveillance systems for ID or AMR (methodological study) or an application of such a tool (case study). All selected articles went through a quality check using the MetaQAT tool. Of 25 articles retrieved, 13 presented a methodological study, while 12 described a case study. Three main types of evaluation were identified through 17 tools: theoretical, process and impact evaluations. Both methodological and case study papers predominantly considered organizational and operational aspects in their evaluation. Although costs and/or impacts were discussed in some case studies, only one article reported an economic impact analysis. Evaluation of One Health integration and multisectoral collaboration was included in four methodological and four case study articles. One major challenge identified in this systematic review is the lack of clear guidance and standardized criteria for the comprehensive evaluation of complex integrated surveillance systems. To overcome this, it is essential to develop, validate, and apply methodologies adapted to these evaluation needs.</div></div>","PeriodicalId":19577,"journal":{"name":"One Health","volume":"20 ","pages":"Article 100995"},"PeriodicalIF":4.1,"publicationDate":"2025-02-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143453975","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The VALUE of antibiotic stewardship for companion animals: Understanding appropriate antibiotic prescribing for pet cats and dogs in veterinary clinics in Singapore","authors":"Huiling Guo ,&nbsp;Zoe Jane-Lara Hildon ,&nbsp;Lok Hang Wong ,&nbsp;Timothy Chua ,&nbsp;Boon Han Teo ,&nbsp;Angela Chow","doi":"10.1016/j.onehlt.2025.100994","DOIUrl":"10.1016/j.onehlt.2025.100994","url":null,"abstract":"<div><h3>Background</h3><div>Understanding the factors influencing antibiotic prescribing for pets can inform future interventions to prevent development and spillover of antimicrobial resistance (AMR) from pets to other animals, humans and the wider environment.</div></div><div><h3>Material and methods</h3><div>We conducted interviews with 19 veterinarians (January<img>July 2022) to explore factors influencing antibiotic prescribing for cats and dogs. Thematic analysis was performed using a VALUE model and themes were segmented by consultation touchpoints.</div></div><div><h3>Results</h3><div>We observed that veterinary clinics in Singapore heavily prioritised business viability. Existing antibiotic stewardship efforts driven by individual veterinarians were often justified as meeting pet owner's satisfaction instead. National guidelines being loosely followed, but AMR-related values and practices were mostly aligned to those of key decision-making veterinarians. Open discussions on antibiotic prescribing amongst different veterinary professionals and shared decision-making (SDM) with pet owners were common. Audits were welcomed by veterinarians but resource limitations were a major concern.</div></div><div><h3>Conclusion</h3><div>Recommendations to support veterinarians in prescribing antibiotics appropriately for cats and dogs range from formalising antibiotic stewardship as a clinic value, providing collective training for all veterinary professionals, continuing SDM with pet owners, automating tracking of meaningful indicators for monitoring and evaluation, and setting up a feedback system to inform behaviour change.</div></div>","PeriodicalId":19577,"journal":{"name":"One Health","volume":"20 ","pages":"Article 100994"},"PeriodicalIF":4.1,"publicationDate":"2025-02-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143421623","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Mapping socioeconomic factors driving antimicrobial resistance in humans: An umbrella review","authors":"Gunnar Ljungqvist ,&nbsp;Robin van Kessel ,&nbsp;Elias Mossialos ,&nbsp;Victoria Saint ,&nbsp;Jelena Schmidt ,&nbsp;Alexander Mafi ,&nbsp;Alison Shutt ,&nbsp;Anuja Chatterjee ,&nbsp;Esmita Charani ,&nbsp;Michael Anderson","doi":"10.1016/j.onehlt.2025.100986","DOIUrl":"10.1016/j.onehlt.2025.100986","url":null,"abstract":"<div><h3>Introduction</h3><div>Antimicrobial resistance (AMR) is one of the biggest public health challenges of our time. National Action Plans have failed so far to effectively address socioeconomic drivers of AMR, including the animal and environmental health dimensions of One Health.</div></div><div><h3>Objective</h3><div>To map what socioeconomic drivers of AMR exist in the literature with quantitative evidence.</div></div><div><h3>Methods</h3><div>An umbrella review was undertaken across Medline, Embase, Global Health, and Cochrane Database of Systematic Reviews, supplemented by a grey literature search on Google Scholar. Review articles demonstrating a methodological search strategy for socioeconomic drivers of AMR were included. Two authors extracted drivers from each review article which were supported by quantitative evidence. Drivers were grouped thematically and summarised narratively across the following three layers of society: People &amp; Public, System &amp; Environment, and Institutions &amp; Policies.</div></div><div><h3>Results</h3><div>The search yielded 6300 articles after deduplication, with 23 review articles included. 27 individual thematic groups of drivers were identified. The People &amp; Public dimensions contained the following themes: age, sex, ethnicity, migrant status, marginalisation, sexual behaviours, socioeconomic status, educational attainment, household composition, maternity, personal hygiene, lifestyle behaviours. System &amp; Environment yielded the following themes: household transmission, healthcare occupation, urbanicity, day-care attendance, environmental hygiene, regional poverty, tourism, animal husbandry, food supply chain, water contamination, and climate. Institutions &amp; Policies encompassed poor antibiotic quality, healthcare financing, healthcare governance, and national income. Many of these contained bidirectional quantitative evidence, hinting at conflicting pathways by which socioeconomic factors drive AMR.</div></div><div><h3>Conclusion</h3><div>This umbrella review maps socioeconomic drivers of AMR with quantitative evidence, providing a macroscopic view of the complex pathways driving AMR. This will help direct future research and action on socioeconomic drivers of AMR.</div></div>","PeriodicalId":19577,"journal":{"name":"One Health","volume":"20 ","pages":"Article 100986"},"PeriodicalIF":4.1,"publicationDate":"2025-02-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143421625","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comprehensive analysis of West Nile Virus transmission: Environmental, ecological, and individual factors. An umbrella review","authors":"Carlos Adrián Vargas Campos ,&nbsp;Selene García-Pérez ,&nbsp;Jordi Figuerola ,&nbsp;Josué Martínez-de la Puente ,&nbsp;Irene Polo ,&nbsp;Belén Rodríguez-de-Fonseca ,&nbsp;Sofía Fernández-Álvarez ,&nbsp;Víctor Galván Fraile ,&nbsp;Marta Martín-Rey ,&nbsp;Marina Lacasaña ,&nbsp;Clara Bermúdez-Tamayo","doi":"10.1016/j.onehlt.2025.100984","DOIUrl":"10.1016/j.onehlt.2025.100984","url":null,"abstract":"<div><h3>Background</h3><div>West Nile Virus (WNV) exemplifies the complexities of managing vector-borne diseases, expanding globally due to human activities and ecological changes. Originating from Africa and transmitted by <em>Culex</em> mosquitoes, WNV is now reported across multiple continents. The aim of this study was to identify the environmental, ecological, and individual factors influencing WNV transmission.</div></div><div><h3>Methods</h3><div>An umbrella review was conducted. Comprehensive searches were performed in PubMed, Scopus, Web of Science, Embase, and LILACS. Inclusion criteria were reviews involving WNV transmission agents (reservoirs, vectors, hosts) and associative analyses between environmental, ecological, or individual factors and WNV transmission. Data extraction and quality appraisal were performed using templates and the AMSTAR 2 tool.</div></div><div><h3>Results</h3><div>From 404 retrieved studies, 23 systematic reviews and meta-analyses were included. Almost 70 % were low or critically low quality. The co-occurrence network highlighted emerging research on climate change and environmental factors. Temperature, precipitation, and land use significantly influence WNV transmission. Warmer temperatures enhance mosquito populations and viral replication, while extreme weather events like droughts increase mosquito-human contact. Climate change significantly contributes to WNV dynamics by altering temperature and precipitation patterns, enhancing vector proliferation, and extending transmission seasons. Ecological factors such as higher avian diversity, vegetation indexes, and distribution of mosquito species can impact WNV transmission significantly. Education and income levels influence preventive behaviors and infection risk, with lower socioeconomic status linked to higher WNV risk. Certain occupational groups are also at elevated risk of WNV infection.</div></div><div><h3>Conclusion</h3><div>Environmental factors like temperature and precipitation critically affect WNV transmission by influencing mosquito behavior and avian reservoir dynamics. Socio-economic status and education levels significantly impact individual preventive behaviors and infection risk. Multifactorial influences on infection risk make necessary integrated surveillance systems and public health strategies. Longitudinal studies with One Health approaches are necessary to better understand WNV dynamics and reduce WNV transmission.</div></div>","PeriodicalId":19577,"journal":{"name":"One Health","volume":"20 ","pages":"Article 100984"},"PeriodicalIF":4.1,"publicationDate":"2025-02-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143429654","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Zoonotic tuberculosis in Catalonia, Spain: Phylogenetic insights into Mycobacterium bovis and M. caprae transmission at the human-livestock interface","authors":"Bernat Pérez de Val ,&nbsp;Enric Vidal ,&nbsp;Tod Stuber ,&nbsp;Jose Luís Sáez ,&nbsp;María Teresa Tórtola","doi":"10.1016/j.onehlt.2025.100993","DOIUrl":"10.1016/j.onehlt.2025.100993","url":null,"abstract":"<div><div>This genomic epidemiology study analyzed <em>Mycobacterium bovis</em> and <em>M. caprae</em> isolates from animals and humans in Catalonia, Spain, between 2005 and 2023. Of 42 human tuberculosis (TB) cases, five were phylogenetically linked to livestock outbreaks, suggesting zoonotic transmission. The findings specifically indicate goats as a primary source of <em>M. caprae</em> transmission to humans. The study also revealed unexpected genetic diversity of mycobacteria within the same outbreak or even within the same patient, highlighting the complexity of tuberculosis transmission dynamics. The results underscore the value of genomic surveillance in understanding zoonotic TB transmission at the human-livestock interface and its role in informing effective control strategies.</div></div>","PeriodicalId":19577,"journal":{"name":"One Health","volume":"20 ","pages":"Article 100993"},"PeriodicalIF":4.1,"publicationDate":"2025-02-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143421624","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Prevalence, antibiotic resistance, resistance and virulence determinants of Campylobacter jejuni in China: A systematic review and meta-analysis","authors":"Zhao Li ,&nbsp;Hua Cai ,&nbsp;Biyao Xu ,&nbsp;Qingli Dong ,&nbsp;Kai Jia ,&nbsp;Zijie Lin ,&nbsp;Xiang Wang ,&nbsp;Yangtai Liu ,&nbsp;Xiaojie Qin","doi":"10.1016/j.onehlt.2025.100990","DOIUrl":"10.1016/j.onehlt.2025.100990","url":null,"abstract":"<div><div><em>Campylobacter jejuni</em> (<em>C. jejuni</em>) is recognized as a serious food contaminant that extensively results in foodborne diseases. Numerous studies have been conducted on the prevalence and antibiotic resistance of <em>C. jejuni</em>, but there is a lack of comprehensive analysis of published data. This study provides a comprehensive overview of the epidemiology, antibiotic resistance, and virulence determinants of <em>C. jejuni</em> in China through a systematic review and meta-analysis. The prevalence levels of <em>C. jejuni</em> from low to high were the humans (5.2 %, 95 % CI: 4.2–6.4 %), foods (12.5 %, 95 % CI: 9.7–15.6 %), animals (15.4 %, 95 % CI: 13.2–17.6 %), and environment (17.8 %, 95 % CI: 9.7–27.7 %), respectively. Furthermore, <em>C. jejuni</em> exhibits high resistance rates to antibiotics such as cefoperazone, nalidixic acid, ciprofloxacin, cefradine, and tetracycline. The overall multi-drug resistance rate (MDR) of <em>C. jejuni</em> was 72.8 % (95 % CI: 62.4–82.2 %), indicating a serious problem with MDR. The resistance of <em>C. jejuni</em> to most antibiotics has increased in the last 20 years. Among the main resistance determinants of <em>C. jejuni</em>, <em>gyrA</em>_T86I and <em>tet</em>(O) had a higher pooled prevalence of 94.8 % (95 % CI: 88.7–99.0 %) and 79.0 % (95 % CI: 66.9–89.2 %), respectively. Furthermore, the high prevalence of virulence-related genes was shown in <em>C. jejuni</em>, such as adhesion (<em>cadF</em>, <em>racR</em>), invasion (<em>ciaB</em>, <em>iamA</em>, <em>ceuE</em>), and toxin (<em>cdtB, cdtC</em>). In summary, <em>C. jejuni</em> has a high prevalence with regional characteristics, and antibiotic resistance of this bacterium especially animal sources remains a serious problem in China. Comprehensive monitoring and control measures for this pathogen are urgently needed to ensure food safety and public health.</div></div>","PeriodicalId":19577,"journal":{"name":"One Health","volume":"20 ","pages":"Article 100990"},"PeriodicalIF":4.1,"publicationDate":"2025-02-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143395533","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Potentially pathogenic free-living amoebae at very high altitude: Detection by multiplex qPCR in the Northern Altiplano fascioliasis hyperendemic area in Bolivia","authors":"Patricia Pérez-Pérez ,&nbsp;Patricio Artigas ,&nbsp;María Reyes-Batlle ,&nbsp;Elizabeth Córdoba-Lanús ,&nbsp;Rubén L. Rodríguez-Expósito ,&nbsp;Pablo F. Cuervo ,&nbsp;Angélica Domínguez-de-Barros ,&nbsp;Omar García-Pérez ,&nbsp;M. Adela Valero ,&nbsp;Alejandra De Elías ,&nbsp;René Anglés ,&nbsp;Santiago Mas-Coma ,&nbsp;José E. Piñero ,&nbsp;M. Dolores Bargues ,&nbsp;Jacob Lorenzo-Morales","doi":"10.1016/j.onehlt.2025.100985","DOIUrl":"10.1016/j.onehlt.2025.100985","url":null,"abstract":"<div><div>Free-living amoebae (FLA), which are frequently found in the environment, include opportunistic pathogenic genera/species such as <em>Acanthamoeba</em> spp., <em>Balamuthia mandrillaris</em>, <em>Naegleria fowleri</em> and <em>Vermamoeba vermiformis</em>. These pathogenic FLA are causative agents of amoebic encephalitis and keratitis in the case of <em>Acanthamoeba</em> genus and <em>V. vermiformis</em>. In addition, amoebic infections are often related to contamination of domestic and recreational water sources. This study aimed to identify potentially pathogenic FLA in the hyperendemic area of human fascioliasis in a very-high-altitude area (3800–4100 m a.s.l.) of Bolivia and examine whether an association between both pathogens could be established from the environmental point of view. A total of 55 samples (28 soil and 27 water samples) were collected from various locations in the Northern Altiplano of Bolivia. Samples were processed by multiplex qPCR to detect the four pathogenic FLA genera/species. All samples were positive for the presence of <em>V. vermiformis</em>, followed by <em>Acanthamoeba</em> spp. which was positive in 18 soil and 10 water samples. In contrast, <em>B. mandrillaris</em> was only detected in soil sources, whereas <em>N. fowleri</em> was not detected in any of the samples. The coexistence and diverse distribution of multiple FLA species in many locations at such a high altitude is worth mentioning and indicates a potential risk of coinfections. These findings suggest that FLA surveillance is a crucial factor to be considered when implementing preventive measures and improving public health in fascioliasis hyperendemic areas.</div></div>","PeriodicalId":19577,"journal":{"name":"One Health","volume":"20 ","pages":"Article 100985"},"PeriodicalIF":4.1,"publicationDate":"2025-02-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143403322","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}