One Health最新文献

{"title":"A review of risk factors at the human-animal-environmental interface of garbage dumps that are driving current and emerging zoonotic diseases","authors":"Nareerat Sangkachai ,&nbsp;Bruce Gummow ,&nbsp;Orachun Hayakijkosol ,&nbsp;Sarin Suwanpakdee ,&nbsp;Anuwat Wiratsudakul","doi":"10.1016/j.onehlt.2024.100915","DOIUrl":"10.1016/j.onehlt.2024.100915","url":null,"abstract":"<div><div>An increasing trend in zoonotic and emerging infectious diseases (EIDs) has been observed worldwide. Most EID outbreaks originate from wildlife, and these outbreaks often involve pathogen–host–environment interaction. Garbage dumps act as an interface between humans, animals, and the environment, from which EIDs could arise. Therefore, this review considers the presence of important pathogens associated with animals and vectors at garbage dumps from a One Health perspective, looking at animal, human, and environmental factors that play a role. A narrative review was performed focusing on four key points, including garbage dumps, animals, waste pickers, zoonoses and EIDs. Articles addressing the presence of terrestrial animals, insects in garbage dumps, and infectious diseases among waste pickers were included in this study. There were 345 relevant articles covering 395 species of terrestrial animals and insects, consisting of 4 species of amphibians, 180 species of birds, 84 species of insects, 114 species of mammals, and 13 species of reptiles. Furthermore, 97 articles (28.12 %) addressed pathogens found in those populations. About half of the articles were interested in bacterial diseases (52.58 %), followed by parasitic diseases (30.93 %) and viral diseases (30.93 %). Zoonotic pathogens were described in 53.6 % of all articles, while 19.59 % focused on drug-resistant microbes, 13.40 % on rodent-borne diseases, and 7.21 % on vector-borne diseases. Garbage dumps would play a role in the emergence of diseases. The relevant factors at garbage dumps that may increase the risk of disease emergence include increased animal populations and density, increased vector population, newly evolved strains of pathogens, increased interaction between humans, domestic animals, wildlife, and vectors, and socio-economic factors. Therefore, sustainable waste management will reduce waste generation, and improve waste collection, and disposal which helps reduce the emergence of new diseases.</div></div>","PeriodicalId":19577,"journal":{"name":"One Health","volume":"19 ","pages":"Article 100915"},"PeriodicalIF":4.1,"publicationDate":"2024-10-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142446514","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Assessment of genetic diversity, tissue tropism, and antigenic properties of Grimsö betacoronavirus in Swedish bank voles (Clethrionomys glareolus)","authors":"Santiago Fernández Morente ,&nbsp;Jinlin Li ,&nbsp;Anishia Wasberg ,&nbsp;Inês R. Faria ,&nbsp;Elin Economou Lundeberg ,&nbsp;Bo Settergren ,&nbsp;Åke Lundkvist ,&nbsp;Jiaxin Ling","doi":"10.1016/j.onehlt.2024.100911","DOIUrl":"10.1016/j.onehlt.2024.100911","url":null,"abstract":"<div><div>Zoonotic coronaviruses can transmit over species barriers and infect humans. To understand the zoonotic potential of a betacoronavirus, Grimsö virus (GRIV), we investigated the geographic distribution and tissue tropism of GRIV in Swedish bank voles (<em>Clethrionomys glareolus</em>), and the antigenicity of the nucleocapsid (N) protein. We screened the lung tissues from animals collected in the southern Sweden by RT-PCR with primers targeting the spike gene. Seven out of 74 animals were found to be positive. They are genetically close to GRIV from Grimsö, central Sweden. Positive rodents were studied for the tissue distribution of GRIV and GRIV RNA was mainly found in the respiratory tract. After three attempts of virus isolation were failed, we successfully established a Vero E6 cell line that stably expressed GRIV N protein, which has no cross-reactivity with patient serum containing antibodies against SARS-CoV-2, or with MERS-CoV. However, a low level of cross-reactivity to common cold coronaviruses was found, likely HCoV-OC43 or HCoV-HKU1, probably due to shared linear epitopes. With the high prevalence and the suggested respiratory transmission route, GRIV may have a high potential for spillover and cross-species transmission, and future serological screening of GRIV infections in domestic animals or humans will be needed.</div></div>","PeriodicalId":19577,"journal":{"name":"One Health","volume":"19 ","pages":"Article 100911"},"PeriodicalIF":4.1,"publicationDate":"2024-10-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142432265","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A one health study on phylogenetics and risk of pathogenic intestinal parasites at a ranch in Inner Mongolia","authors":"Ziran Mo ,&nbsp;Jingwei Quan ,&nbsp;Bin Xu ,&nbsp;Huixia Yu ,&nbsp;Junyan Li ,&nbsp;Xiaoping Luo ,&nbsp;Qimuge Wuyun ,&nbsp;Jian Li ,&nbsp;Wenbin Yang ,&nbsp;Wei Hu","doi":"10.1016/j.onehlt.2024.100912","DOIUrl":"10.1016/j.onehlt.2024.100912","url":null,"abstract":"<div><div><em>Cryptosporidium</em>, <em>Giardia duodenalis</em>, and <em>Enterocytozoon bieneusi</em> are widespread zoonotic pathogens causing gastrointestinal diseases in humans and various animal species. Inner Mongolia, a major beef production region in China, there is a notable absence of comprehensive research on intestinal parasitism. Thus, timely and comprehensive diagnosis is essential to mitigate disease spread and minimize economic losses in the livestock industry. In this study, we collected fecal samples from cattle and humans, as well as soil and water samples, and all samples were tested for pathogenic intestinal protozoa at the Simmental cattle ranch in Wengniute, Chifeng City, Inner Mongolia. Among the 393 samples tested, 76/371 (20.5 %) cattle, 6/11 (54.5 %) ranch workers, 1/7 (14.3 %) water, and 2/4 (50 %) soil samples were positive. Factors affecting the infection rate of intestinal protozoa were examined. Results showed that the infection rate was higher in June than in January, higher in calves than in adults, and higher in diarrheal calves than in healthy calves. Additionally, the infection rate of intestinal protozoa was higher in pathogen-contaminated water source sheds than in uncontaminated sheds. Genetic and evolutionary analyses revealed that the prevalent <em>E. bieneusi</em> subtypes are predominantly J, I, and BEB4, while the <em>G. duodenalis</em> subtypes are assemblages B and E. The <em>Cryptosporidium</em> species identified were <em>C. bovis</em>, <em>C. andersoni</em>, <em>C. parvum</em>, <em>C. ryanae</em>, and <em>C. suis</em>, with <em>C. parvum</em> being a notable zoonotic pathogen. The pathogen sequences from humans, cattle, water, and soil showed 99–100 % similarity, suggesting possible transmission or contamination between animals and the environment. This study contributes to the One Health approach by addressing the gap in research on intestinal protozoa in Inner Mongolia. It provides important data for other ranches in the region to understand the prevalence of such pathogens and develop effective control measures. Using the concept of One Health to analyze the spatiotemporal distribution of intestinal protozoa in pastures is of great significance for maintaining public health.</div></div>","PeriodicalId":19577,"journal":{"name":"One Health","volume":"19 ","pages":"Article 100912"},"PeriodicalIF":4.1,"publicationDate":"2024-10-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142432267","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Antimicrobial resistance in Escherichia coli and Staphylococcus aureus at human-animal interfaces on Chongming Island, Shanghai: A One Health perspective","authors":"Chao Lv ,&nbsp;Jun Leng ,&nbsp;Minjian Qian ,&nbsp;Bingqing Sun ,&nbsp;HuiPing Ye ,&nbsp;Min Li ,&nbsp;Nan Zhou ,&nbsp;Zile Cheng ,&nbsp;Yiwen Chen ,&nbsp;Xiaokui Guo ,&nbsp;Jun Shang ,&nbsp;Li Zhang ,&nbsp;Yongzhang Zhu","doi":"10.1016/j.onehlt.2024.100910","DOIUrl":"10.1016/j.onehlt.2024.100910","url":null,"abstract":"<div><div>Antimicrobial resistance (AMR) is a significant concern within the One Health framework due to its ability to spread across multiple interfaces. Phenotypic data remains the primary type for AMR surveillance, but exploring association across multiple interfaces poses certain challenges. In this study, AMR phenotypic data of clinical and food animal <em>E. coli</em> and <em>S. aureus</em> from Chongming Island over the past five years were analyzed to determine key characteristics of AMR and explore its association at the human-animal interface.</div><div>The clinical <em>E. coli</em> isolates showed significant resistance to penicillins (83.92 %), cephems (63.05 %), fluoroquinolones (62.21 %), and tetracyclines (57.77 %), while <em>S. aureus</em> exhibited high resistance to penicillinase-labile penicillins (90.89 %), macrolides (51.51 %), penicillinase-stable penicillins (43.96 %), and lincosamides (43.55 %). Extended-spectrum β-lactamase (ESBL)-producing <em>E. coli</em> isolates accounted for 53.26 % (1398/2526), while methicillin-resistant <em>Staphylococcus aureus</em> (MRSA) prevalence was 43.81 % (435/993). Notably, there has been an increase in the proportion of <em>E. coli</em> isolates resistant to 8 to 12 antimicrobial classes, and in the proportion of <em>S. aureus</em> isolates resistant to 5 to 9 classes. Certain multi-drug resistance (MDR) phenotypes were first identified in food animal isolates and later emerged in clinical settings. Meanwhile, several MDR phenotypes were shared between the two interfaces, with 44 identified in <em>E. coli</em> and 12 in <em>S. aureus</em>. Further co-occurrence analysis in <em>E. coli</em> and <em>S. aureus</em> identified several co-occurrence phenotypic pairs or clusters, potentially mediated by a single plasmid or multiple plasmids within a bacterium, indicating potential associations at the human-animal interface.</div><div>To summarize, a heightened prevalence of MDR in clinical <em>E. coli</em> and <em>S. aureus</em> has been observed, with some MDR profiles appearing in food animals before emerging in clinical settings. The co-occurrence of phenotypic pairs or clusters underscores the potential for AMR association and transmission between humans and food animals. Within the One Health framework, integrating genomic data into AMR monitoring is a crucial next step.</div></div>","PeriodicalId":19577,"journal":{"name":"One Health","volume":"19 ","pages":"Article 100910"},"PeriodicalIF":4.1,"publicationDate":"2024-10-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142432272","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Identification of common spatial and temporal trends in the epidemiology of cattle bovine tuberculosis and human extrapulmonary and drug-resistant tuberculosis in Malawi","authors":"Alfred Ngwira ,&nbsp;Samuel Manda ,&nbsp;Esron Daniel Karimuribo ,&nbsp;Sharadhuli Iddi Kimera","doi":"10.1016/j.onehlt.2024.100905","DOIUrl":"10.1016/j.onehlt.2024.100905","url":null,"abstract":"<div><h3>Background</h3><div>Identification of common spatial disease trends between cattle bovine tuberculosis (BTB) and human extrapulmonary tuberculosis (EPTB) and drug-resistant tuberculosis (DRTB) can support integrated disease control and monitoring programmes. We employed the recently developed multivariate disease mapping methods to examine whether the diseases exhibited any spatial correlation.</div></div><div><h3>Methods</h3><div>A retrospective study of cattle BTB and human EPTB and DRTB cases from 2018 to 2022 was conducted. Bivariate shared spatiotemporal components models were fitted to a) cattle BTB and human EPTB and b) cattle BTB and human DRTB at the district level in Malawi, with cattle density, human density and climatic variables as independent variables.</div></div><div><h3>Results</h3><div>Disease specific spatial effects were higher in the southern half of the country, while the shared spatial effects were more dominant in both the south and western parts of the country. The shared temporal effects showed constant trends, while disease specific temporal effects showed an increasing pattern for cattle BTB and a constant pattern for human EPTB and DRTB. The predicted disease incidence pattern for all forms of TB in the period without data showed a constant pattern over the years. Cattle density was positively associated with cattle BTB (<span><math><mi>β</mi></math></span>: 0.022; 95% Credible Interval (CI): 0.004, 0.042). Human density was positively associated with human EPTB (<span><math><mi>β</mi></math></span>: 0.005; 95% CI: 0.001, 0.009).</div></div><div><h3>Conclusion</h3><div>Cattle BTB and human EPTB and DRTB have a common spatial pattern in the west and southern parts of Malawi. Integrated interventions targeting high-density areas for cattle and human may have positive impacts on cattle BTB and human EPTB and DRTB.</div></div>","PeriodicalId":19577,"journal":{"name":"One Health","volume":"19 ","pages":"Article 100905"},"PeriodicalIF":4.1,"publicationDate":"2024-10-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142437702","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Serological prevalence of Brucella spp. at the livestock-human interface in Jordan","authors":"Ehab A. Abu-Basha ,&nbsp;Zuhair Bani Ismail ,&nbsp;Lea Widemann ,&nbsp;Yasmin Daradkeh ,&nbsp;Omar Al-Omari ,&nbsp;Alaa Fahmawi ,&nbsp;Mais Lakaideh ,&nbsp;Belal Sha'fout ,&nbsp;Haia Mellhem ,&nbsp;Leen Al-Bayari ,&nbsp;Hani Talafha ,&nbsp;Zaidoun Hijazeen ,&nbsp;Bilal Al-Omari ,&nbsp;Jean DeMarco ,&nbsp;William B. Karesh","doi":"10.1016/j.onehlt.2024.100906","DOIUrl":"10.1016/j.onehlt.2024.100906","url":null,"abstract":"<div><div>Despite its endemic status in the Middle East, key knowledge gaps persist regarding the prevalence, transmission rate, and geographical distribution of both human and livestock brucellosis in Jordan. This study aimed to investigate the seroprevalence of human and livestock brucellosis as well as the incidence of brucellosis in humans in Jordan. A total of 500 human participants (202 exposed and 296 unexposed to livestock) were enrolled in the study. Sampling was conducted at baseline and 1.5 years later. Additionally, a total of 700 livestock were sampled, comprising 20 animals per taxa (camels, cattle, sheep, goats) per site, at both baseline (<em>N</em> = 350) and the 1.5-year follow-up (N = 350). Human participants were longitudinally followed, whereas livestock sampling was conducted opportunistically. Blood samples obtained from both humans and livestock at baseline and follow-up were tested for <em>Brucella</em> spp. serum antibodies using the Rose Bengal test (RBT) and complement fixation test (CFT). The overall seroprevalence of brucellosis in humans at baseline was 3.4 % (95 % CI: 2.0–5.4). Positive test results in humans were detected from all five sites with no significant regional variation observed. Seroprevalence was higher in individuals regularly exposed to livestock (6.1 %; 95 % CI: 3.5–9.9) compared to those not regularly exposed (0.80 %; 95 % CI: 0.10–2.9). Incidence of human brucellosis was 924 seropositives per 100,000 person-years, with all incident seropositives occurring in the livestock-exposed cohort. In livestock, the overall seroprevalence of brucellosis was 5.4 % (95 % CI: 3.5–8.3) at baseline compared to 2.6 % (95 % CI: 1.4–4.8) at follow-up. Seropositive livestock were detected at all sites apart from Al-Zarqa, and in all species apart from camels. In conclusion: Brucellosis burden was higher among humans regularly exposed to livestock, re-emphasizing the need for disease control in livestock populations to prevent primary infection in humans.</div></div>","PeriodicalId":19577,"journal":{"name":"One Health","volume":"19 ","pages":"Article 100906"},"PeriodicalIF":4.1,"publicationDate":"2024-10-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142432271","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A first insight into seropositivity and risk factors for Brucella spp. and Coxiella burnetii in free-roaming dogs in Ecuador","authors":"Angel Sebastian Rodriguez-Pazmiño ,&nbsp;Carla M. Brito ,&nbsp;Mauricio Salas-Rueda ,&nbsp;Solon Alberto Orlando ,&nbsp;Miguel Angel Garcia-Bereguiain","doi":"10.1016/j.onehlt.2024.100909","DOIUrl":"10.1016/j.onehlt.2024.100909","url":null,"abstract":"<div><div>Brucellosis and Q fever are two bacterial zoonoses caused by <em>Brucella</em> spp. and <em>Coxiella burnetii</em>, respectively. Dogs are reservoirs of these pathogens and play an important role in their spread. In this research, we determined the seroprevalence of antibodies against <em>Brucella</em> spp. and <em>C. burnetii</em> in free-roaming dogs from Ecuador and conducted a statistical analysis based on geographical variables. Serum samples were collected from 397 free-roaming dogs between November 2018 and May 2019 and analyzed with commercial ELISA tests for <em>Brucella</em> spp. and Q fever. An overall seroprevalence of 2.8 % (CI: 95 %, 0.0–6.2 %) and 1.8 % (CI: 95 %, 0.0–5.6 %) was found for <em>Brucella</em> spp. and <em>C. burnetii</em>, respectively. No statistical differences in seroprevalence values were found between geographical regions in Ecuador or between dogs from rural or urban settings, except for the association of <em>C. burnetii</em> infection with the Coastal Region. This is the first study of this kind in Ecuador and points out the need for a One Health approach for control and surveillance of zoonotic diseases like brucellosis and Q fever including feral and stray dogs as reservoirs to spread those pathogens to cattle, humans, or wildlife.</div></div>","PeriodicalId":19577,"journal":{"name":"One Health","volume":"19 ","pages":"Article 100909"},"PeriodicalIF":4.1,"publicationDate":"2024-10-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142432266","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Echinococcus granulosus complex infection in wild boar hunters and auxiliary hunting dogs","authors":"Mariaelisa Carbonara ,&nbsp;Francesco Buono ,&nbsp;Anna Morea ,&nbsp;Giovanni Sgroi ,&nbsp;Maria Paola Maurelli ,&nbsp;Francesco Locantore ,&nbsp;Paolo Trerotoli ,&nbsp;Francesca Indraccolo ,&nbsp;Angela Stufano ,&nbsp;Valentina Schino ,&nbsp;Nicola D'Alessio ,&nbsp;Vincenzo Veneziano ,&nbsp;Piero Lovreglio ,&nbsp;Domenico Otranto ,&nbsp;Roberta Iatta","doi":"10.1016/j.onehlt.2024.100908","DOIUrl":"10.1016/j.onehlt.2024.100908","url":null,"abstract":"<div><div>Cystic echinococcosis (CE) caused by <em>Echinococcus granulosus</em> sensu lato (s.l.) is a zoonotic neglected tropical disease endemic in Italy, which perpetuates in several intermediate hosts, including wild boars, and dogs as definitive hosts. People living in rural and livestock-raising areas are exposed to <em>E. granulosus</em> s.l. infection, as well as people leading outdoor activities in endemic regions. Therefore, this study was designed to assess the exposure to <em>Echinococcus</em> spp. in wild boar hunters, the role of their hunting dogs as parasite reservoirs, along with hunter's knowledge on the infection risk.</div><div>From December 2022 to May 2023, wild boar hunters (<em>n</em> = 122) from southern Italy were recruited on volunteer basis for blood and serum sampling and a questionnaire enquiring socio-demographic, anamnestic data and knowledge on CE was also filled out. Sera were tested for <em>Echinococcus</em> spp. IgG by a commercial enzyme-linked immunosorbent assay (Euroimmun ELISA®, Germany). In addition, faecal samples from their hunting dogs (<em>n</em> = 208) were screened for Taeniidae eggs by parasitological and molecular approaches.</div><div>Overall, six (4.9 %) hunters scored either positive or borderline for IgG anti-<em>Echinococcus</em> spp., of which one presented a calcified hepatic cyst at abdominal ultrasonography. In addition, 6.3 % Taeniidae prevalence was recorded in faecal samples (13/208) of hunting dogs, and <em>E. granulosus</em> sensu stricto (s.s.) was molecularly identified in two samples. The statistical analysis revealed the risk factors (odds ratio &gt; 1, <em>p</em> &lt; 0.05) associated with parasitic exposure, including the hunter geographical provenience, and the presence of animals around or in the house.</div><div>The <em>E. granulosus</em> s.l. exposure of hunters herein detected, coupled with the parasite molecular positivity of their hunting dogs and the limited awareness on <em>Echinococcus</em> spp. life cycle/infection risk, highlight the relevance to promote health surveillance and educational programs within the hunting category, for minimizing the cestode circulation in the wildlife-urban premises.</div></div>","PeriodicalId":19577,"journal":{"name":"One Health","volume":"19 ","pages":"Article 100908"},"PeriodicalIF":4.1,"publicationDate":"2024-10-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142432268","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Pan-azole resistance in clinical Aspergillus fumigatus isolates carrying TR34/L98H from birds and mammals in Belgium","authors":"Hanne Debergh ,&nbsp;Roel Haesendonck ,&nbsp;Nadine Botteldoorn ,&nbsp;An Martel ,&nbsp;Frank Pasmans ,&nbsp;Claude Saegerman ,&nbsp;Ann Packeu","doi":"10.1016/j.onehlt.2024.100907","DOIUrl":"10.1016/j.onehlt.2024.100907","url":null,"abstract":"<div><div>Aspergillosis causes significant health risks to both birds and mammals. The outcome of these infections is often poor due to delayed diagnosis and treatment failure. We investigated 152 cases of aspergillosis from birds and mammals in Belgium. Most samples originated from the taxonomic orders Artiodactyla (40.1 %) and Columbiformes (19.7 %). Five isolates (3.3 %) showed phenotypical resistance against at least one medical azole. Three of these isolates were pan-azole resistant bearing the TR34/L98H mutation. The predominance of this resistance mutation supports an environmental route for exposure and resistance selection, highlighting the importance of the One Health concept.</div></div>","PeriodicalId":19577,"journal":{"name":"One Health","volume":"19 ","pages":"Article 100907"},"PeriodicalIF":4.1,"publicationDate":"2024-10-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142432270","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Exploring AMR and virulence in Klebsiella pneumoniae isolated from humans and pet animals: A complement of phenotype by WGS-derived profiles in a One Health study in Egypt","authors":"Enas A. Soliman ,&nbsp;Alaa Saad ,&nbsp;Ashraf A. Abd El Tawab ,&nbsp;Fatma I. Elhofy ,&nbsp;Amira M. Rizk ,&nbsp;Manar Elkhayat ,&nbsp;Tamara Kozytska ,&nbsp;Majdil Ilyas ,&nbsp;Marwa Bassiouny ,&nbsp;Hanka Brangsch ,&nbsp;Mathias W. Pletz ,&nbsp;Heinrich Neubauer ,&nbsp;Lisa D. Sprague ,&nbsp;Gamal Wareth","doi":"10.1016/j.onehlt.2024.100904","DOIUrl":"10.1016/j.onehlt.2024.100904","url":null,"abstract":"<div><div><em>Klebsiella pneumoniae</em> is a ubiquitous nosocomial pathogen associated with various types of infections in hospitalized patients and different animal species. In the current study, 49 <em>Klebsiella</em> strains isolated from humans, dogs, and cats were investigated using NGS technology. MALDI-TOF failed to identify newly discovered <em>K. variicola</em> and <em>K. quasipneumoniae</em> isolates correctly. MLST analysis revealed different sequence types among <em>K. pneumoniae</em> isolates, and the most frequent STs were ST29, ST219, and ST37. Three ST23 that are generally known as hypervirulent type were identified but they lacked major discriminatory determinants for hypervirulent <em>K. pneumoniae</em> (hvKp). <em>K. pneumoniae</em> isolates showed high diversity, and several isolates from humans and animals were assigned to the same ST and were almost identical. Isolates from humans exhibited more pronounced resistance patterns compared to the animal isolates. High levels of resistance were observed for piperacillin, trimethoprim/sulfamethoxazole, and cephalosporins, and resistance to carbapenem compounds was only found in isolates of human origin. Three strains of human origin were extensively drug-resistant (XDR). A diverse range of resistance genes primarily confer resistance to beta-lactams., phenicol/quinolone, aminoglycoside, macrolide, sulfonamides, and fosfomycin were identified in silico<em>.</em> However, there were inconsistencies between the phenotypic characterization of isolates and the set of resistance genes detected in silico in this set of <em>Klebsiella</em> isolates. Further research using a larger number of isolates from various sources is necessary to fully comprehend the relationship between the presence of antimicrobial resistance determinants and phenotypic data. It is also necessary to monitor the spread of <em>K. pneumoniae</em> from a One Health perspective in Egypt.</div></div>","PeriodicalId":19577,"journal":{"name":"One Health","volume":"19 ","pages":"Article 100904"},"PeriodicalIF":4.1,"publicationDate":"2024-09-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142432269","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}