NPJ Science of Food最新文献_第6页

Integrated E-nose, volatilomics, and transcriptomics analysis of the aroma formation mechanism during pitaya fruit ripening. 综合电子鼻、挥发物学和转录组学分析火龙果成熟过程中香气形成机制。

IF 9.7 1区农林科学

NPJ Science of Food Pub Date : 2026-07-05 DOI: 10.1038/s41538-026-00981-7

Liangjie Ba, Xiaoling Long, Chenglin Luo, Sen Cao, Jianye Chen, Donglan Luo

{"title":"Integrated E-nose, volatilomics, and transcriptomics analysis of the aroma formation mechanism during pitaya fruit ripening.","authors":"Liangjie Ba, Xiaoling Long, Chenglin Luo, Sen Cao, Jianye Chen, Donglan Luo","doi":"10.1038/s41538-026-00981-7","DOIUrl":"https://doi.org/10.1038/s41538-026-00981-7","url":null,"abstract":"Pitaya fruit is of high nutritional and economic value, yet the molecular mechanisms governing its aroma formation during ripening remain poorly understood. This study employed an integrated approach combining E-nose, HS-SPME-GC-MS, and transcriptomics to investigate the formation and dynamic changes of volatile organic compounds (VOCs) and their underlying molecular mechanisms in \"Zihonglong\" pitaya from Guizhou Province, China. A total of 856 VOCs were tentatively identified, among which terpenoids, esters, and ketones were the predominant chemical groups. Based on relative odor activity values (rOAV > 1), 79 compounds were identified as key contributors to aroma perception. Transcriptomic analysis indicated that α-linolenic acid metabolism, terpenoid biosynthesis, and phenylpropanoid biosynthesis were closely associated with volatile formation during ripening. Weighted gene co-expression network analysis (WGCNA) further revealed two major regulatory modules, designated \"Turquoise\" and \"Blue\", which showed strong correlations with the accumulation patterns of characteristic aroma metabolites. Multivariate analysis of E-nose responses successfully distinguished unripe fruit from ripening stages, demonstrating its potential for rapid maturity screening. This study provides mechanistic insights into aroma evolution during pitaya ripening and supports the development of aroma-based quality evaluation strategies.","PeriodicalId":19367,"journal":{"name":"NPJ Science of Food","volume":" ","pages":""},"PeriodicalIF":9.7,"publicationDate":"2026-07-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148387869","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Sulfated polysaccharide from Gelidium crinale: modulation of angiogenesis and fibrosis in skin repair. 海参硫酸盐多糖：调节皮肤修复中的血管生成和纤维化。

IF 9.7 1区农林科学

NPJ Science of Food Pub Date : 2026-07-04 DOI: 10.1038/s41538-026-00977-3

Yi Liu, Haiyan Zheng, Yizhu Chen, Bomi Ryu, Zhong-Ji Qian

{"title":"Sulfated polysaccharide from Gelidium crinale: modulation of angiogenesis and fibrosis in skin repair.","authors":"Yi Liu, Haiyan Zheng, Yizhu Chen, Bomi Ryu, Zhong-Ji Qian","doi":"10.1038/s41538-026-00977-3","DOIUrl":"https://doi.org/10.1038/s41538-026-00977-3","url":null,"abstract":"Scar tissue formation is a common pathological outcome of skin repair after injury, characterized by excessive collagen deposition and closely associated with inflammation and angiogenesis. This study investigated the effects and related signaling changes of a food-derived sulfated polysaccharide from the red alga Gelidium crinale (GNP) on angiogenesis and scar formation using in vitro and in vivo models. Human umbilical vein endothelial cells (HUVECs) and human keloid fibroblasts (HKFs) were used to evaluate the biological activity of GNP, while an OSA-CMCS self-crosslinking hydrogel was employed as a delivery vehicle to improve local administration in a mouse wound-healing model. GNP significantly inhibited HUVEC migration, invasion, and tube formation, accompanied by reduced expression of angiogenesis-related factors, including PDGF, ANG-II, and HIF-1α, suggesting impaired angiogenic activation. In HKFs, GNP decreased TGF-β1 expression and modulated downstream fibrosis-related signaling, thereby suppressing collagen and fibronectin production. Moreover, incorporation of GNP into the OSA-CMCS hydrogel reduced inflammatory responses, angiogenesis, and collagen deposition in vivo. These findings demonstrate the potential of GNP as a natural anti-scar candidate and provide a promising strategy for the high-value utilization of Gelidium crinale polysaccharides.","PeriodicalId":19367,"journal":{"name":"NPJ Science of Food","volume":" ","pages":""},"PeriodicalIF":9.7,"publicationDate":"2026-07-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148387779","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Multi-omics profiling of high-carotenoid hybrid potato lines reveals coordinated metabolic reprogramming and associates with distinct tuber microbiota. 高类胡萝卜素杂交马铃薯系的多组学分析揭示了协调的代谢重编程，并与不同的块茎微生物群有关。

IF 9.7 1区农林科学

NPJ Science of Food Pub Date : 2026-07-04 DOI: 10.1038/s41538-026-00842-3

Bo Zhang, Yiming Zhong, Blaise Pascal Muvunyi, Tingting Xu, Jiawei Liu, Xingyao Xiong, Xu Cheng

{"title":"Multi-omics profiling of high-carotenoid hybrid potato lines reveals coordinated metabolic reprogramming and associates with distinct tuber microbiota.","authors":"Bo Zhang, Yiming Zhong, Blaise Pascal Muvunyi, Tingting Xu, Jiawei Liu, Xingyao Xiong, Xu Cheng","doi":"10.1038/s41538-026-00842-3","DOIUrl":"10.1038/s41538-026-00842-3","url":null,"abstract":"Potato is a critical staple crop, and enhancing its carotenoid content is a promising strategy to improve its nutritional value. However, the synergistic mechanisms underlying carotenoid accumulation, superior nutritional traits, and the role of the endophytic microbiome remain unclear. Using an integrated multi-omics strategy, we systematically analyzed two high-zeaxanthin/lutein hybrids and four commercial cultivars. The hybrids accumulated significantly higher levels of zeaxanthin, lutein, and minerals, while exhibiting superior processing traits (e.g., higher dry matter/starch, lower reducing sugars). Integrated metabolomic and transcriptomic profiling revealed a coordinated upregulation of carotenoid and phenylpropanoid biosynthesis, alongside enrichment of stress-responsive phenolic acids. Notably, the endophytic microbiome in high-carotenoid tubers was distinct, dominated by Firmicutes and Proteobacteria, with genera like Bacillus and Latilactobacillus positively correlating with carotenoid content. Weighted gene co-expression network analysis identified a core regulatory module containing key genes (e.g., CCD4, BCH2) and novel transcription factors. Our findings elucidate a synergistic network linking metabolism, gene regulation, and the endophytic microbiome that collectively is associated with carotenoid accumulation and tuber quality. This provides critical targets for breeding nutritionally enhanced potatoes with desirable agronomic performance, supporting nutritional security and sustainable agriculture.","PeriodicalId":19367,"journal":{"name":"NPJ Science of Food","volume":" ","pages":""},"PeriodicalIF":9.7,"publicationDate":"2026-07-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13338389/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148387781","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Lactiplantibacillus plantarum SLpl116 attenuates OVA-induced food allergy with ecological restoration of the gut microbiota and immune rebalancing. 植物乳杆菌SLpl116通过肠道菌群的生态恢复和免疫平衡来减轻ova诱导的食物过敏。

IF 9.7 1区农林科学

NPJ Science of Food Pub Date : 2026-07-04 DOI: 10.1038/s41538-026-00978-2

Soyeon Jin, You-Tae Kim, Jihye Yang, Ju-Hoon Lee

{"title":"Lactiplantibacillus plantarum SLpl116 attenuates OVA-induced food allergy with ecological restoration of the gut microbiota and immune rebalancing.","authors":"Soyeon Jin, You-Tae Kim, Jihye Yang, Ju-Hoon Lee","doi":"10.1038/s41538-026-00978-2","DOIUrl":"10.1038/s41538-026-00978-2","url":null,"abstract":"Gut dysbiosis is increasingly recognized as a key contributor to food allergy, yet probiotic strains capable of restoring allergic microbiota and rebalancing host immunity remain limited. Here, we identified Lactiplantibacillus plantarum SLpl116 through a multi-criteria screening pipeline integrating anti-allergic activity, safety, and processing stability, and evaluated its efficacy in a prophylactic ovalbumin (OVA)-induced murine food allergy model. SLpl116 significantly attenuated allergic symptoms, including diarrhea and hypothermia, and suppressed serum IgE, IgG1, OVA-specific immunoglobulins, and mucosal mast cell protease-1. It was also associated with suppression of Th2-related responses and enhancement of systemic Th1-associated signaling, indicating restoration of Th1/Th2 immune balance. Microbiome analysis showed that SLpl116 was associated with ecological restoration of the dysbiotic gut community, including suppression of allergy-associated taxa such as Alistipes finegoldii and Bacteroides and enrichment of beneficial commensals, particularly Lachnospiraceae. Correlation analysis supported an association between microbial reconfiguration and immune rebalancing, while PICRUSt2-based functional prediction suggested enriched butyrate-associated metabolic potential in the effective strain groups. Comparative genome-informed analysis further indicated that SLpl116 possessed distinctive phenotype-linked features, providing a plausible molecular rationale for its favorable phenotype. Together, these findings identify SLpl116 as a promising strain-level probiotic candidate associated with direct immune rebalancing and microbiome-associated ecological restoration.","PeriodicalId":19367,"journal":{"name":"NPJ Science of Food","volume":" ","pages":""},"PeriodicalIF":9.7,"publicationDate":"2026-07-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13558680/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148387818","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Targeting DNA methylation: molecular and in vivo evidence for quinoa and chia nanocapsules as epigenetic modulators in DMBA-treated rats. 靶向DNA甲基化：藜麦和奇亚纳米胶囊作为dba处理大鼠表观遗传调节剂的分子和体内证据。

IF 9.7 1区农林科学

NPJ Science of Food Pub Date : 2026-07-03 DOI: 10.1038/s41538-026-00946-w

Dalia M Mabrouk, Mohamed M Abd Elrahman, Tamer K Khatab, Aida I El Makawy

引用次数: 0

Next-generation structured lipid systems in plant-based meat: oleogels, emulsion architectures, and sensory optimization. 下一代植物性肉类结构脂质系统：油凝胶，乳液结构和感官优化。

IF 9.7 1区农林科学

NPJ Science of Food Pub Date : 2026-07-03 DOI: 10.1038/s41538-026-00950-0

Sadaqat Ali, Anand Kumar, Benish Ali, Gita Rani, Hammad Ali Fadlalmola, Saad Alghamdi, Asgar Ali, Li Zhang

引用次数: 0

Structure-property relationships in agar-soy protein emulsion gels: effects of oil type and droplet size. 琼脂-大豆蛋白乳液凝胶的结构-性质关系：油类型和液滴大小的影响。

IF 9.7 1区农林科学

NPJ Science of Food Pub Date : 2026-07-03 DOI: 10.1038/s41538-026-00966-6

Jiseon Lee, Minyeong Lee, Youling L Xiong, Mi-Jung Choi

{"title":"Structure-property relationships in agar-soy protein emulsion gels: effects of oil type and droplet size.","authors":"Jiseon Lee, Minyeong Lee, Youling L Xiong, Mi-Jung Choi","doi":"10.1038/s41538-026-00966-6","DOIUrl":"https://doi.org/10.1038/s41538-026-00966-6","url":null,"abstract":"This study investigated agar-soy protein isolate emulsion gels, focusing on the effects of oil type and droplet size on their structural organization and physicochemical properties. Emulsion gels were prepared using canola oil, rice bran oil, or eugenol in micro-sized and nano-sized forms, and their properties were evaluated in comparison with pork fat. Nano-sized emulsions produced finer, more homogeneous networks, while micro-sized emulsions showed higher mechanical strength. Rice bran oil nano-sized gels exhibited balanced strength, high water-holding capacity, and low syneresis, indicating enhanced network compactness and stability. These effects may be associated with differences in interfacial characteristics and droplet-matrix interactions depending on oil type. Eugenol-based gels showed structure-dependent behavior, where micro-sized gels enhanced hardness and freeze-thaw resistance, whereas nano-sized gels exhibited reduced structural integrity. Thermal analysis confirmed the absence of triglyceride melting transitions, although rice bran oil nano-sized gels showed the highest transition enthalpy. FTIR analysis suggested size-dependent differences in molecular organization within the gel network, reflecting variations in interfacial structuring and lipid domain distribution. These findings highlight the potential of combining droplet size control with oil phase characteristics to design structured emulsion gel systems for plant-based applications.","PeriodicalId":19367,"journal":{"name":"NPJ Science of Food","volume":" ","pages":""},"PeriodicalIF":9.7,"publicationDate":"2026-07-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148382396","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Integrated lipidomics and fatty acid positional distribution for nutritional assessment of nine mammalian milks. 综合脂质组学和脂肪酸位置分布对9种哺乳动物奶的营养评价。

IF 9.7 1区农林科学

NPJ Science of Food Pub Date : 2026-07-03 DOI: 10.1038/s41538-026-00954-w

Meiqing Chen, Songcui Lu, Lin Song, Jun Zhang, Yingjie Qi, Xiaowei Zhao, Nan Zheng, Yangdong Zhang, Jiaqi Wang

{"title":"Integrated lipidomics and fatty acid positional distribution for nutritional assessment of nine mammalian milks.","authors":"Meiqing Chen, Songcui Lu, Lin Song, Jun Zhang, Yingjie Qi, Xiaowei Zhao, Nan Zheng, Yangdong Zhang, Jiaqi Wang","doi":"10.1038/s41538-026-00954-w","DOIUrl":"https://doi.org/10.1038/s41538-026-00954-w","url":null,"abstract":"The nutritional value of milk fat depends on lipid and fatty acid composition. This study characterized the lipidomics and fatty acid positional distribution of milk from nine mammalian species: cow, buffalo, yak, goat, sheep, camel, mare, donkey, and human. Lipid molecular species were profiled by liquid chromatography-tandem mass spectrometry. Fatty acid positional distribution was determined using NH₂ and Si solid-phase extraction, selective sn-1/3 hydrolysis by Candida antarctica lipase B, and sn-2 monoacylglycerol quantification by gas chromatography-mass spectrometry. Ruminant milks showed high compositional similarity and differed from camel and monogastric milks. Compared to human milk, animal milks had higher triacylglycerols, phosphatidylcholines, phosphatidylethanolamines, and sphingomyelins, but lower free fatty acids and diacylglycerols. Short-chain fatty acids consistently occupied sn-1/3 positions, whereas medium-chain, long-chain, odd-chain, and branched-chain fatty acids favored sn-2 position. Monogastric milks exhibited sn-2 preference for palmitic acid and other saturated fatty acids and sn-1/3 preference for unsaturated fatty acids; ruminant and camel milks showed the reverse. Bray-Curtis analysis identified donkey milk as the closest lipidomic analog to human milk, followed by mare milk. These findings provide a systematic reference for evaluating mammalian milks and data for humanized infant formula lipid design.","PeriodicalId":19367,"journal":{"name":"NPJ Science of Food","volume":" ","pages":""},"PeriodicalIF":9.7,"publicationDate":"2026-07-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148382441","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Innovative magnetic effervescence‑assisted microextraction for ensuring food safety: rapid monitoring of pyrethroid residues in Chrysanthemum morifolium Ramat. 创新磁泡辅助微萃取确保食品安全：菊花中拟除虫菊酯残留的快速监测。

IF 9.7 1区农林科学

NPJ Science of Food Pub Date : 2026-07-03 DOI: 10.1038/s41538-026-00969-3

Gaotian Li, Menglei Wang, Zheyuan Xu, Hongqing Wang, Guofang Shen, Zhi Yang, Hailong Xiao, Jinhua Yan, Xuming Ji, Jiazhao Ruan

{"title":"Innovative magnetic effervescence‑assisted microextraction for ensuring food safety: rapid monitoring of pyrethroid residues in Chrysanthemum morifolium Ramat.","authors":"Gaotian Li, Menglei Wang, Zheyuan Xu, Hongqing Wang, Guofang Shen, Zhi Yang, Hailong Xiao, Jinhua Yan, Xuming Ji, Jiazhao Ruan","doi":"10.1038/s41538-026-00969-3","DOIUrl":"10.1038/s41538-026-00969-3","url":null,"abstract":"Ensuring the safety of widely consumed floral tea requires robust methods for monitoring pesticide residues. Herein, an innovative magnetic effervescence-assisted liquid-liquid extraction (MEALLE) method was developed for the rapid detection of pyrethroids in Chrysanthemum morifolium Ramat (C. morifolium). Effervescent tablets containing CO2/H+ donors, Fe3O4, and carboxylated multi-walled carbon nanotubes were prepared via one-pot grinding-and-pressing preparation. During extraction, the tablet was introduced into C. morifolium powder pre-moistened with acidified acetonitrile as the extracting solvent. By integrating effervescence, salting-out, and magnetic aggregation, this method simplified the sample preparation procedure, eliminated the need for vortexing and centrifugation, and reduced the total time to approximately 3 min. Coupled with gas chromatography-tandem mass spectrometry (GC-MS/MS), the method exhibited excellent linearity (R2 > 0.998), sensitivity (limits of quantification: 1.6-4.1 μg kg-1), accuracy (recovery: 73.9-108.7%), and precision (relative standard deviations < 5.7%). Application of the MEALLE method to commercial C. morifolium samples further validated its robustness and versatility. Overall, this approach provide a time-effective, equipment-independent solution for pesticide residue analysis that is independent of auxiliary equipment during the sample pretreatment, thereby serving as a valuable tool for ensuring the safety of processed floral products.","PeriodicalId":19367,"journal":{"name":"NPJ Science of Food","volume":" ","pages":""},"PeriodicalIF":9.7,"publicationDate":"2026-07-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13578300/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148382439","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Multi-omics reveals mechanism of combined L-cysteine and GABA in loquat postharvest quality. 多组学揭示了l -半胱氨酸和GABA联合作用对枇杷采后品质的影响机制。

IF 9.7 1区农林科学

NPJ Science of Food Pub Date : 2026-07-03 DOI: 10.1038/s41538-026-00940-2

Huifen Zhang, Jing Pu, Miao Wang, Ying Du, Xiaofu Tang, Jie Niu, Hongxia Wang, Xian Luo, Yongqing Wang, Qunxian Deng

{"title":"Multi-omics reveals mechanism of combined L-cysteine and GABA in loquat postharvest quality.","authors":"Huifen Zhang, Jing Pu, Miao Wang, Ying Du, Xiaofu Tang, Jie Niu, Hongxia Wang, Xian Luo, Yongqing Wang, Qunxian Deng","doi":"10.1038/s41538-026-00940-2","DOIUrl":"https://doi.org/10.1038/s41538-026-00940-2","url":null,"abstract":"Loquat is highly susceptible to postharvest deterioration, including browning and lignification, which limits its storage life. This study investigated the effects of combined L-cysteine (L-Cys) and γ-aminobutyric acid (GABA) treatment on postharvest quality maintenance of loquat fruit and elucidated the underlying molecular mechanisms through integrated transcriptomic and metabolomic analyses. Physiological assessments revealed that the combined treatment significantly delayed fruit softening, reduced weight loss and browning, suppressed decay incidence, and maintained higher levels of soluble solids, titratable acidity, and total phenolics. Integrated multi-omics analysis demonstrated that L-Cys+GABA treatment primarily modulated the phenylpropanoid metabolic pathway, leading to suppressed lignin biosynthesis through coordinated downregulation of key biosynthetic genes (COMT, CCR, LAC) and reduced accumulation of corresponding metabolites. These findings reveal that L-Cys+GABA combined treatment delays postharvest senescence in loquat fruit by targeting the phenylpropanoid pathway to inhibit lignification, providing a physiological and molecular basis for its application as an effective preservation strategy.","PeriodicalId":19367,"journal":{"name":"NPJ Science of Food","volume":" ","pages":""},"PeriodicalIF":9.7,"publicationDate":"2026-07-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148382428","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0