NPJ Science of Food最新文献

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Cell-based, cell-cultured, cell-cultivated, cultured, or cultivated. What is the best name for meat, poultry, and seafood made directly from the cells of animals? 细胞的,细胞培养的,细胞培养的,培养的或培养的直接由动物细胞制成的肉、家禽和海鲜的最佳名称是什么?
IF 6.4 1区 农林科学
NPJ Science of Food Pub Date : 2023-12-06 DOI: 10.1038/s41538-023-00234-x
William K. Hallman, William K. Hallman II, Eileen E. Hallman
{"title":"Cell-based, cell-cultured, cell-cultivated, cultured, or cultivated. What is the best name for meat, poultry, and seafood made directly from the cells of animals?","authors":"William K. Hallman, William K. Hallman II, Eileen E. Hallman","doi":"10.1038/s41538-023-00234-x","DOIUrl":"10.1038/s41538-023-00234-x","url":null,"abstract":"To be sold in the United States, meat, poultry, and seafood products made from cultured cells must be labeled with a “common or usual name” to help consumers understand what they are purchasing. The terms “Cultured,” “Cultivated,” “Cell-Cultured,” “Cell-Cultivated,” “Cell-Based” and a control (without a common or usual name) were tested using an online experiment. Two regulatory criteria were assessed: that the term distinguishes the novel products from conventional products, and appropriately signals allergenicity. Three consumer acceptance criteria were assessed: that the term is seen as appropriate, does not disparage the novel or conventional products, nor elicit perceptions that the products are unsafe, unhealthy, or not nutritious. Each term was shown on packages of frozen Beef Filets, Beef Burgers, Chicken Breasts, Chicken Burgers, Atlantic Salmon Fillets, and Salmon Burgers. A representative sample of 4385 Americans (18 + ) were randomly assigned to view a single product with a single term or the control. Consumers’ ability to distinguish tested terms from conventional products differed by product category. “Cultured” and “Cultivated” failed to adequately differentiate the novel products from “Wild-Caught and Farm-Raised” salmon products. “Cultivated” failed to differentiate the novel Beef Filet product from “Grass-Fed” Beef Filets. “Cell-Cultured,” “Cell-Cultivated,” and “Cell-Based” each signaled that the products were different from conventional products across the proteins, and signaled allergenicity, meeting the two key regulatory criteria. They were not significantly different on most consumer perception measures. However, “Cell-Cultured” may have slightly better consumer acceptance across the novel beef, chicken, and salmon products, recommending its universal adoption.","PeriodicalId":19367,"journal":{"name":"NPJ Science of Food","volume":null,"pages":null},"PeriodicalIF":6.4,"publicationDate":"2023-12-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10700563/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138499024","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Sustainable healthy diet modeling for a plant-based dietary transitioning in the United States 美国植物性饮食转型的可持续健康饮食模型。
IF 6.4 1区 农林科学
NPJ Science of Food Pub Date : 2023-11-28 DOI: 10.1038/s41538-023-00239-6
Raphael Aidoo, Vincent Abe-Inge, Ebenezer M. Kwofie, Jamie I. Baum, Stan Kubow
{"title":"Sustainable healthy diet modeling for a plant-based dietary transitioning in the United States","authors":"Raphael Aidoo, Vincent Abe-Inge, Ebenezer M. Kwofie, Jamie I. Baum, Stan Kubow","doi":"10.1038/s41538-023-00239-6","DOIUrl":"10.1038/s41538-023-00239-6","url":null,"abstract":"The potential environmental and nutritional benefits of plant-based dietary shifts require thorough investigation to outline suitable routes to achieve these benefits. Whereas dietary consumption is usually in composite forms, sustainable healthy diet assessments have not adequately addressed composite diets. In this study, we build on available data in the Food4HealthyLife calculator to develop 3 dietary concepts (M) containing 24 model composite diet scenarios (S) assessed for their environmental and nutritional performances. The Health Nutritional Index (HENI) and Food Compass scoring systems were used for nutritional quality profiling and estimates of environmental impact were derived from previously reported midpoint impact values for foods listed in the What We Eat in America database. The diets were ranked using the Kruskal‒Wallis nonparametric test, and a dual-scale data chart was employed for a trade-off analysis to identify the optimal composite diet scenario. The results showcased a distinct variation in ranks for each scenario on the environment and nutrition scales, describing an inherent nonlinear relationship between environmental and nutritional performances. However, trade-off analysis revealed a diet with 10% legumes, 0.11% red meat, 0.28% processed meat and 2.81% white meat could reduce global warming by 54.72% while yielding a diet quality of 74.13 on the Food Compass Scoring system. These observations provide an interesting forecast of the benefits of transitioning to an optimal plant- and animal-based dieting pattern, which advances global nutritional needs and environmental stewardship among consumers.","PeriodicalId":19367,"journal":{"name":"NPJ Science of Food","volume":null,"pages":null},"PeriodicalIF":6.4,"publicationDate":"2023-11-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10684880/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138452059","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
An integrated model for pre- and post-harvest aflatoxin contamination in maize 玉米采前与采后黄曲霉毒素污染综合模型研究。
IF 6.4 1区 农林科学
NPJ Science of Food Pub Date : 2023-11-18 DOI: 10.1038/s41538-023-00238-7
Richard O.J.H. Stutt, Matthew D. Castle, Peter Markwell, Robert Baker, Christopher A. Gilligan
{"title":"An integrated model for pre- and post-harvest aflatoxin contamination in maize","authors":"Richard O.J.H. Stutt, Matthew D. Castle, Peter Markwell, Robert Baker, Christopher A. Gilligan","doi":"10.1038/s41538-023-00238-7","DOIUrl":"10.1038/s41538-023-00238-7","url":null,"abstract":"Aflatoxin contamination caused by colonization of maize by Aspergillus flavus continues to pose a major human and livestock health hazard in the food chain. Increasing attention has been focused on the development of models to predict risk and to identify effective intervention strategies. Most risk prediction models have focused on elucidating weather and site variables on the pre-harvest dynamics of A. flavus growth and aflatoxin production. However fungal growth and toxin accumulation continue to occur after harvest, especially in countries where storage conditions are limited by logistical and cost constraints. In this paper, building on previous work, we introduce and test an integrated meteorology-driven epidemiological model that covers the entire supply chain from planting to delivery. We parameterise the model using approximate Bayesian computation with monthly time-series data over six years for contamination levels of aflatoxin in daily shipments received from up to three sourcing regions at a high-volume maize processing plant in South Central India. The time series for aflatoxin levels from the parameterised model successfully replicated the overall profile, scale and variance of the historical aflatoxin datasets used for fitting and validation. We use the model to illustrate the dynamics of A. flavus growth and aflatoxin production during the pre- and post-harvest phases in different sourcing regions, in short-term predictions to inform decision making about sourcing supplies and to compare intervention strategies to reduce the risks of aflatoxin contamination.","PeriodicalId":19367,"journal":{"name":"NPJ Science of Food","volume":null,"pages":null},"PeriodicalIF":6.4,"publicationDate":"2023-11-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10657429/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138047730","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Composition of gut microbiota involved in alleviation of dexamethasone-induced muscle atrophy by whey protein 肠道微生物群的组成与乳清蛋白减轻地塞米松诱导的肌肉萎缩有关。
IF 6.4 1区 农林科学
NPJ Science of Food Pub Date : 2023-11-01 DOI: 10.1038/s41538-023-00235-w
JinLing Qiu, Yixing Cheng, Yang Deng, Guangxu Ren, Jiaqi Wang
{"title":"Composition of gut microbiota involved in alleviation of dexamethasone-induced muscle atrophy by whey protein","authors":"JinLing Qiu, Yixing Cheng, Yang Deng, Guangxu Ren, Jiaqi Wang","doi":"10.1038/s41538-023-00235-w","DOIUrl":"10.1038/s41538-023-00235-w","url":null,"abstract":"Skeletal muscle atrophy is a condition associated with increased morbidity and mortality. While the concept of the gut-muscle axis has been proposed, the role of gut microbiota in dexamethasone (DEX)-induced skeletal muscle atrophy remains largely unknown, limiting its clinical applications. In this study, we found that administration of DEX caused a shift in the gut microbiota of mice, characterized by an increased ratio of Firmicutes/Bacteroidota and a reduction in alpha diversity. We also identified 480 new operational taxonomic units (OTUs), while 1168 specific OTUs were lost. Our Spearman correlation analysis revealed 28 key taxonomic genera of bacteria that were positively or negatively associated with skeletal muscle strength and weight (r: −0.881 to 0.845, p < 0.05). Moreover, supplementation with whey protein reshaped the gut microbiota structure in DEX-treated mice, making it more similar to that of the control group. Importantly, we further utilized a stepwise regression model to identify two enterotypes capable of predicting skeletal muscle function and weight. Notably, Ileibacterium and Lachnospiraceae_UCG-001 played significant roles in predicting both skeletal muscle function and weight. Our findings suggest that DEX causes shifts in the gut microbiota, which can be reversed by whey protein intervention. The enterotypes identified by our stepwise regression models predict muscle function and weight, underscoring the potential role of gut microbiota in modulating muscle atrophy and emphasizing the therapeutic opportunities of microbiota-altering interventions.","PeriodicalId":19367,"journal":{"name":"NPJ Science of Food","volume":null,"pages":null},"PeriodicalIF":6.4,"publicationDate":"2023-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10618183/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"71425540","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Postprandial glycemic and lipidemic effects of black rice anthocyanin extract fortification in foods of varying macronutrient compositions and matrices 在不同常量营养成分和基质的食物中强化黑米花青素提取物对餐后血糖和血脂的影响。
IF 6.4 1区 农林科学
NPJ Science of Food Pub Date : 2023-11-01 DOI: 10.1038/s41538-023-00233-y
Sean Jun Leong Ou, Dimeng Yang, Hanny Putri Pranata, E Shyong Tai, Mei Hui Liu
{"title":"Postprandial glycemic and lipidemic effects of black rice anthocyanin extract fortification in foods of varying macronutrient compositions and matrices","authors":"Sean Jun Leong Ou, Dimeng Yang, Hanny Putri Pranata, E Shyong Tai, Mei Hui Liu","doi":"10.1038/s41538-023-00233-y","DOIUrl":"10.1038/s41538-023-00233-y","url":null,"abstract":"Anthocyanin (ACN) fortification of commonly consumed foods is significant as a dietary strategy against the development of metabolic complications by delivering ACNs at high doses. However, its bioactivity and translated metabolic effects in the presence of varying food matrices and macro-constituents is particularly unclear. This end-to-end study investigates the metabolic effects of black rice ACN extract (BRAE) fortification—from in-vitro enzyme inhibitory activities and digestibility, to downstream in vivo impacts on GI, postprandial glycemia and lipidemia. The in vivo effects were investigated in two separate crossover randomised controlled trials (RCT) of 24 healthy participants each—the first RCT determined the postprandial blood glucose, insulin, and ACN bioavailability to a starch-rich single food over 2 h, while the second RCT determined the postprandial blood glucose, insulin, lipid panel, and lipoprotein particles and subfractions to a starch- and fat-rich composite meal over 4 h. In-vitro findings confirmed the inhibitory activities of major black rice ACNs on carbohydrases (p = 0.0004), lipases (p = 0.0002), and starch digestibility (p < 0.0001). in vivo, a 27-point mean GI reduction of wheat bread was observed with BRAE fortification, despite a non-significant attenuation in postprandial glycemia. Conversely, there were no differences in postprandial glycemia when fortified bread was consumed as a composite meal, but acute lipid profiles were altered: (1) improved plasma HDL-c, ([0.0140 mmol/L, 95% CI: (0.00639, 0.0216)], p = 0.0028), Apo-A1 ([0.0296 mmol/L, 95% CI: (0.00757, 0.0515)], p = 0.0203), and Apo-B ([0.00880 mmol/L, 95% CI: (0.00243, 0.0152)], p = 0.0185), (2) modified LDL and HDL subfractions (p < 0.05), and (3) remodelled lipid distributions in HDL and LDL particles. This end-to-end study indicates the potential of ACN fortification in GI reduction and modulating postprandial lipoprotein profiles to starch- and fat-rich composite meals.","PeriodicalId":19367,"journal":{"name":"NPJ Science of Food","volume":null,"pages":null},"PeriodicalIF":6.4,"publicationDate":"2023-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10620212/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"71425541","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Author Correction: Applying federated learning to combat food fraud in food supply chains 作者更正:应用联合学习打击食品供应链中的食品欺诈。
IF 6.4 1区 农林科学
NPJ Science of Food Pub Date : 2023-10-19 DOI: 10.1038/s41538-023-00232-z
Anand Gavai, Yamine Bouzembrak, Wenjuan Mu, Frank Martin, Rajaram Kaliyaperumal, Johan van Soest, Ananya Choudhury, Jaap Heringa, Andre Dekker, Hans J. P. Marvin
{"title":"Author Correction: Applying federated learning to combat food fraud in food supply chains","authors":"Anand Gavai, Yamine Bouzembrak, Wenjuan Mu, Frank Martin, Rajaram Kaliyaperumal, Johan van Soest, Ananya Choudhury, Jaap Heringa, Andre Dekker, Hans J. P. Marvin","doi":"10.1038/s41538-023-00232-z","DOIUrl":"10.1038/s41538-023-00232-z","url":null,"abstract":"","PeriodicalId":19367,"journal":{"name":"NPJ Science of Food","volume":null,"pages":null},"PeriodicalIF":6.4,"publicationDate":"2023-10-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10587136/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49680362","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Lipid complexation reduces rice starch digestibility and boosts short-chain fatty acid production via gut microbiota 脂质络合降低了大米淀粉的消化率,并通过肠道微生物群促进短链脂肪酸的产生。
IF 6.4 1区 农林科学
NPJ Science of Food Pub Date : 2023-10-18 DOI: 10.1038/s41538-023-00230-1
Yi Shen, Zengxu An, Zongyao Huyan, Xiaoli Shu, Dianxing Wu, Ning Zhang, Nicoletta Pellegrini, Josep Rubert
{"title":"Lipid complexation reduces rice starch digestibility and boosts short-chain fatty acid production via gut microbiota","authors":"Yi Shen, Zengxu An, Zongyao Huyan, Xiaoli Shu, Dianxing Wu, Ning Zhang, Nicoletta Pellegrini, Josep Rubert","doi":"10.1038/s41538-023-00230-1","DOIUrl":"10.1038/s41538-023-00230-1","url":null,"abstract":"In this study, two rice varieties (RS4 and GZ93) with different amylose and lipid contents were studied, and their starch was used to prepare starch-palmitic acid complexes. The RS4 samples showed a significantly higher lipid content in their flour, starch, and complex samples compared to GZ93. The static in vitro digestion highlighted that RS4 samples had significantly lower digestibility than the GZ93 samples. The C∞ of the starch-lipid complex samples was found to be 17.7% and 18.5% lower than that of the starch samples in GZ93 and RS4, respectively. The INFOGEST undigested fractions were subsequently used for in vitro colonic fermentation. Short-chain fatty acids (SCFAs) concentrations, mainly acetate, and propionate were significantly higher in starch-lipid complexes compared to native flour or starch samples. Starch-lipid complexes produced a distinctive microbial composition, which resulted in different gene functions, mainly related to pyruvate, fructose, and mannose metabolism. Using Model-based Integration of Metabolite Observations and Species Abundances 2 (MIMOSA2), SCFA production was predicted and associated with the gut microbiota. These results indicated that incorporating lipids into rice starch promotes SCFA production by modulating the gut microbiota selectively.","PeriodicalId":19367,"journal":{"name":"NPJ Science of Food","volume":null,"pages":null},"PeriodicalIF":6.4,"publicationDate":"2023-10-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10584848/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49680363","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Spatially localised expression of the glutamate decarboxylase gadB in Escherichia coli O157:H7 microcolonies in hydrogel matrices 谷氨酸脱羧酶gadB在水凝胶基质中大肠杆菌O157:H7微菌落中的空间定位表达。
IF 6.4 1区 农林科学
NPJ Science of Food Pub Date : 2023-10-14 DOI: 10.1038/s41538-023-00229-8
Cédric Saint Martin, Nelly Caccia, Maud Darsonval, Marina Gregoire, Arthur Combeau, Grégory Jubelin, Florence Dubois-Brissonnet, Sabine Leroy, Romain Briandet, Mickaël Desvaux
{"title":"Spatially localised expression of the glutamate decarboxylase gadB in Escherichia coli O157:H7 microcolonies in hydrogel matrices","authors":"Cédric Saint Martin, Nelly Caccia, Maud Darsonval, Marina Gregoire, Arthur Combeau, Grégory Jubelin, Florence Dubois-Brissonnet, Sabine Leroy, Romain Briandet, Mickaël Desvaux","doi":"10.1038/s41538-023-00229-8","DOIUrl":"10.1038/s41538-023-00229-8","url":null,"abstract":"Functional diversity within isogenic spatially organised bacterial populations has been shown to trigger emergent community properties such as stress tolerance. Considering gadB gene encoding a key glutamate decarboxylase involved in E. coli tolerance to acidic conditions, we investigated its expression in hydrogels mimicking the texture of some structured food matrices (such as minced meat or soft cheese). Taking advantage of confocal laser scanning microscopy combined with a genetically-engineered dual fluorescent reporter system, it was possible to visualise the spatial patterns of bacterial gene expression from in-gel microcolonies. In E. coli O157:H7 microcolonies, gadB showed radically different expression patterns between neutral (pH 7) or acidic (pH 5) hydrogels. Differential spatial expression was determined in acidic hydrogels with a strong expression of gadB at the microcolony periphery. Strikingly, very similar spatial patterns of gadB expression were further observed for E. coli O157:H7 grown in the presence of L. lactis. Considering the ingestion of contaminated foodstuff, survival of E. coli O157:H7 to acidic stomachal stress (pH 2) was significantly increased for bacterial cells grown in microcolonies in acidic hydrogels compared to planktonic cells. These findings have significant implications for risk assessment and public health as they highlight inherent differences in bacterial physiology and virulence between liquid and structured food products. The contrasting characteristics observed underscore the need to consider the distinct challenges posed by these food types, thereby emphasising the importance of tailored risk mitigation strategies.","PeriodicalId":19367,"journal":{"name":"NPJ Science of Food","volume":null,"pages":null},"PeriodicalIF":6.4,"publicationDate":"2023-10-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10576782/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41207320","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Awakening the natural capability of psicose production in Escherichia coli 唤醒大肠杆菌产生灵菌素的天然能力。
IF 6.4 1区 农林科学
NPJ Science of Food Pub Date : 2023-10-14 DOI: 10.1038/s41538-023-00231-0
Jayce E. Taylor, Dileep Sai Kumar Palur, Angela Zhang, Jake N. Gonzales, Augustine Arredondo, Timothy A. Coulther, Amiruddin Bin Johan Lechner, Elys P. Rodriguez, Oliver Fiehn, John Didzbalis, Justin B. Siegel, Shota Atsumi
{"title":"Awakening the natural capability of psicose production in Escherichia coli","authors":"Jayce E. Taylor, Dileep Sai Kumar Palur, Angela Zhang, Jake N. Gonzales, Augustine Arredondo, Timothy A. Coulther, Amiruddin Bin Johan Lechner, Elys P. Rodriguez, Oliver Fiehn, John Didzbalis, Justin B. Siegel, Shota Atsumi","doi":"10.1038/s41538-023-00231-0","DOIUrl":"10.1038/s41538-023-00231-0","url":null,"abstract":"Due to the rampant rise in obesity and diabetes, consumers are desperately seeking for ways to reduce their sugar intake, but to date there are no options that are both accessible and without sacrifice of palatability. One of the most promising new ingredients in the food system as a non-nutritive sugar substitute with near perfect palatability is D-psicose. D-psicose is currently produced using an in vitro enzymatic isomerization of D-fructose, resulting in low yield and purity, and therefore requiring substantial downstream processing to obtain a high purity product. This has made adoption of D-psicose into products limited and results in significantly higher per unit costs, reducing accessibility to those most in need. Here, we found that Escherichia coli natively possesses a thermodynamically favorable pathway to produce D-psicose from D-glucose through a series of phosphorylation-epimerization-dephosphorylation steps. To increase carbon flux towards D-psicose production, we introduced a series of genetic modifications to pathway enzymes, central carbon metabolism, and competing metabolic pathways. In an attempt to maximize both cellular viability and D-psicose production, we implemented methods for the dynamic regulation of key genes including clustered regularly interspaced short palindromic repeats inhibition (CRISPRi) and stationary-phase promoters. The engineered strains achieved complete consumption of D-glucose and production of D-psicose, at a titer of 15.3 g L-1, productivity of 2 g L-1 h-1, and yield of 62% under test tube conditions. These results demonstrate the viability of whole-cell catalysis as a sustainable alternative to in vitro enzymatic synthesis for the accessible production of D-psicose.","PeriodicalId":19367,"journal":{"name":"NPJ Science of Food","volume":null,"pages":null},"PeriodicalIF":6.4,"publicationDate":"2023-10-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10576766/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41207319","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Effects of sodium nitrite reduction, removal or replacement on cured and cooked meat for microbiological growth, food safety, colon ecosystem, and colorectal carcinogenesis in Fischer 344 rats 亚硝酸钠还原、去除或替代对Fischer 344大鼠腌制和烹饪肉类的微生物生长、食品安全、结肠生态系统和结直肠癌的影响。
IF 6.4 1区 农林科学
NPJ Science of Food Pub Date : 2023-10-07 DOI: 10.1038/s41538-023-00228-9
Françoise Guéraud, Charline Buisson, Aurélie Promeyrat, Nathalie Naud, Edwin Fouché, Valérie Bézirard, Jacques Dupuy, Pascale Plaisancié, Cécile Héliès-Toussaint, Lidwine Trouilh, Jean-Luc Martin, Sabine Jeuge, Eléna Keuleyan, Noémie Petit, Laurent Aubry, Vassilia Théodorou, Bastien Frémaux, Maïwenn Olier, Giovanna Caderni, Tina Kostka, Gilles Nassy, Véronique Santé-Lhoutellier, Fabrice Pierre
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