NPJ Science of Food最新文献_第5页

Key metabolic pathways of the aging process of Citri Grandis Exocarpium and its identified potential metabolites associated with antioxidant activity. 柑桔衰老过程的关键代谢途径及其与抗氧化活性相关的潜在代谢物。

IF 6.3 1区农林科学

NPJ Science of Food Pub Date : 2025-04-24 DOI: 10.1038/s41538-025-00411-0

Xiaoting Zhu, Jun Li, Gengsheng Xiao, Jijun Wu, Yuanshan Yu, Jing Wen, Zhibin Bu, Xiaowei Chen, Wai San Cheang, Yujuan Xu, Manqin Fu

{"title":"Key metabolic pathways of the aging process of Citri Grandis Exocarpium and its identified potential metabolites associated with antioxidant activity.","authors":"Xiaoting Zhu, Jun Li, Gengsheng Xiao, Jijun Wu, Yuanshan Yu, Jing Wen, Zhibin Bu, Xiaowei Chen, Wai San Cheang, Yujuan Xu, Manqin Fu","doi":"10.1038/s41538-025-00411-0","DOIUrl":"https://doi.org/10.1038/s41538-025-00411-0","url":null,"abstract":"Citri Grandis Exocarpium (CGE), is renowned for its dual use in medicine and food, being well-known for its beneficial antioxidant and anti-inflammatory effects. However, the changes of chemical composition and antioxidant activity of CGE during the storage (aging) process are still unclear. In this study, We examined the changes in metabolites of CGE samples aged for 1 year (CG1), 3 years (CG3) and 5 years (CG5), mainly through comprehensive metabolomics techniques. The results indicated that analysis of CGE extracts by ultra-high-performance liquid chromatography-quadrupole/time of flight mass spectrometry (UHPLC-QTOF-MS/MS) resulted in the detection of 1249 metabolites. A total of 57 differential metabolites were identified as potential markers for distinguishing CGEs of different aging years by chemometrics methods, including naringin, among others. The KEGG annotation, enrichment and topology analysis revealed that the biosynthesis of flavonoids was a key metabolic pathway during the aging process of CEG, involving 14 flavonoids determined as differential metabolites. Total flavonoids, naringin, naringenin, butin, 7',4-dihydroxyflavone, and phlorizin have been identified as potential metabolites associated with antioxidant activity. These results clarified the effects of aging time on the flavonoids accumulation and antioxidant activity of CGE, and provided a scientific basis for understanding the mechanism of CGE aging process.","PeriodicalId":19367,"journal":{"name":"NPJ Science of Food","volume":"9 1","pages":"57"},"PeriodicalIF":6.3,"publicationDate":"2025-04-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12022338/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144032578","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Identification of ice-binding proteins from Raphanus sativus and application in frozen dough. 萝卜冰结合蛋白的鉴定及其在冷冻面团中的应用。

IF 6.3 1区农林科学

NPJ Science of Food Pub Date : 2025-04-24 DOI: 10.1038/s41538-025-00420-z

Shu-Heng Zhao, Jing Tan, Wei Zhang, Yan Zhou, Yi-Qiu Ning, Yue Sun, Jun-Wei Zhao, De-Ming Jiang, Xiao-Fang Li

{"title":"Identification of ice-binding proteins from Raphanus sativus and application in frozen dough.","authors":"Shu-Heng Zhao, Jing Tan, Wei Zhang, Yan Zhou, Yi-Qiu Ning, Yue Sun, Jun-Wei Zhao, De-Ming Jiang, Xiao-Fang Li","doi":"10.1038/s41538-025-00420-z","DOIUrl":"https://doi.org/10.1038/s41538-025-00420-z","url":null,"abstract":"Cryopreservation is a widely employed method for processing and preserving food. However, conventional antifreeze agents often hard to mitigate the mechanical damage caused by ice recrystallization during freeze-thaw cycles. In this study, two ice-binding proteins (IBPs), COR15B and COR47, were identified from Raphanus sativus using bioinformatics and molecular biology techniques. Both IBPs exhibited significant ice recrystallization inhibition (IRI) and ice crystal morphology modification activity. A novel, high-yield Bacillus subtilis expression system was developed for the heterologous production of these IBPs, achieving approximately 50 μg/mL through response surface optimization. These proteins, even when used at thousandths of the ratio, retained their IRI activity. Notably, the heterologously expressed IBPs significantly reduced freeze-induced damage in flour-based products and improved yeast survival and fermentative capacity during repeated freeze-thaw cycles. These results highlight the considerable potential of radish-derived IBPs as cryoprotectants for enhancing food storage stability.","PeriodicalId":19367,"journal":{"name":"NPJ Science of Food","volume":"9 1","pages":"58"},"PeriodicalIF":6.3,"publicationDate":"2025-04-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12022278/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144021017","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Long-term GABA supplementation mitigates anxiety by modulating complement and neuroinflammatory pathways. 长期补充GABA可通过调节补体和神经炎症途径减轻焦虑。

IF 6.3 1区农林科学

NPJ Science of Food Pub Date : 2025-04-24 DOI: 10.1038/s41538-025-00423-w

Jiyi Xu, Ziyu Ge, Han Wang, Chenhui Zhang, Jinjie Xu, Ying Li, Xiangyun Yang, Ling Zhang, Zhanjiang Li, Zhe Liu, Gang Wang, Jing Du

{"title":"Long-term GABA supplementation mitigates anxiety by modulating complement and neuroinflammatory pathways.","authors":"Jiyi Xu, Ziyu Ge, Han Wang, Chenhui Zhang, Jinjie Xu, Ying Li, Xiangyun Yang, Ling Zhang, Zhanjiang Li, Zhe Liu, Gang Wang, Jing Du","doi":"10.1038/s41538-025-00423-w","DOIUrl":"https://doi.org/10.1038/s41538-025-00423-w","url":null,"abstract":"Anxiety disorders are among the most prevalent mental health conditions, often linked with neuroinflammation and imbalances in neurotransmitter systems. This study examined the anxiolytic effects of oral GABA in chronic restraint stress (CRS) mice. Mice were divided into control, CRS, and two GABA-treated groups (10 mg/kg, 20 mg/kg). After 14 days of administration, anxiety-like behaviors were assessed using elevated-plus maze and open-field tests. GABA levels in the prefrontal cortex were quantified via ELISA, while anti-inflammatory cytokines were measured using an antibody array. Proteomic analysis of the hippocampus identified differentially expressed proteins, validated through Parallel Reaction Monitoring and immunoblotting. Results showed that GABA significantly alleviated anxiety-like behaviors, increased GABA levels in the prefrontal cortex, and elevated anti-inflammatory factors IL-10 and TGF-β1. Proteomic analysis and validation revealed GABA reversed complement dysregulation (C3, C4b, Cfh, Cfi). These findings suggest GABA alleviates anxiety by modulating immune homeostasis and complement activation, highlighting its therapeutic potential.","PeriodicalId":19367,"journal":{"name":"NPJ Science of Food","volume":"9 1","pages":"60"},"PeriodicalIF":6.3,"publicationDate":"2025-04-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12022253/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144034732","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Utilizing drip metabolites and predictive modeling for non-destructive freshness assessment in pork loin. 利用滴漏代谢物和预测模型对猪里脊肉进行无损新鲜度评价。

IF 6.3 1区农林科学

NPJ Science of Food Pub Date : 2025-04-23 DOI: 10.1038/s41538-025-00421-y

Hyun-Jun Kim, Hye-Jin Kim, Heesang Hong, Minwoo Choi, Azfar Ismail, Daye Mun, Younghoon Kim, Gap-Don Kim, Cheorun Jo

{"title":"Utilizing drip metabolites and predictive modeling for non-destructive freshness assessment in pork loin.","authors":"Hyun-Jun Kim, Hye-Jin Kim, Heesang Hong, Minwoo Choi, Azfar Ismail, Daye Mun, Younghoon Kim, Gap-Don Kim, Cheorun Jo","doi":"10.1038/s41538-025-00421-y","DOIUrl":"https://doi.org/10.1038/s41538-025-00421-y","url":null,"abstract":"This study validated the use of pork drip metabolites for non-destructive freshness prediction. The pork loin was vacuum-packaged and stored for 27 days at 4 °C. The pH, drip loss, total aerobic bacterial counts (TAB), microbial composition and drip metabolites were examined. LASSO and Random Forest (RF) were selected and used for variable selection, while Ridge regression and Support Vector Regression were utilized to develop predictive models. Validation was performed using leave-one-out cross-validation. LASSO and RF selected 13 and 10 metabolites, respectively. The metabolites selected by each method were trained using Ridge regression and SVR. Each of the four trained models achieved R2 values of over 0.9. In the validation step, the model trained by Ridge regression using drip metabolites selected through LASSO showed the lowest RMSE value of 0.283 log CFU/g. Therefore, selected drip metabolites can be used to predict TAB and microbial composition of pork loin through mathematical modeling.","PeriodicalId":19367,"journal":{"name":"NPJ Science of Food","volume":"9 1","pages":"55"},"PeriodicalIF":6.3,"publicationDate":"2025-04-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12019311/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144032191","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Defining the role of processing in food classification systems-the IUFoST formulation & processing approach. 定义加工在食品分类系统中的作用- IUFoST的制定和加工方法。

IF 6.3 1区农林科学

NPJ Science of Food Pub Date : 2025-04-23 DOI: 10.1038/s41538-025-00395-x

Lilia Ahrné, Hongda Chen, Christiani J Henry, Hyun-Sook Kim, Barbara Schneeman, Erich J Windhab

引用次数: 0

Regulatory effects of Poria cocos polysaccharides on gut microbiota and metabolites: evaluation of prebiotic potential. 茯苓多糖对肠道菌群和代谢物的调节作用：益生元潜力的评价。

IF 6.3 1区农林科学

NPJ Science of Food Pub Date : 2025-04-22 DOI: 10.1038/s41538-025-00416-9

Xu Zhou, Yangyou Li, Yuhan Yang, Li Wei, Chen Wang, Jiahui Xu, Jiajia Song, Shijian Liu, Junying Bai, Huayi Suo

{"title":"Regulatory effects of Poria cocos polysaccharides on gut microbiota and metabolites: evaluation of prebiotic potential.","authors":"Xu Zhou, Yangyou Li, Yuhan Yang, Li Wei, Chen Wang, Jiahui Xu, Jiajia Song, Shijian Liu, Junying Bai, Huayi Suo","doi":"10.1038/s41538-025-00416-9","DOIUrl":"https://doi.org/10.1038/s41538-025-00416-9","url":null,"abstract":"Dietary polysaccharides have long been recognized for their capacity to modulate gut microbiota composition and metabolic activities, making them promising resources for functional food development. In this study, we investigated the effects of Poria cocos (P. cocos) polysaccharides on the structure and metabolism of human gut microbiota using an in vitro fecal fermentation model. Our results revealed that P. cocos polysaccharides were readily utilized by the gut microbiota, as evidenced by a significant decrease in pH and an increase in SCFAs concentrations. Notably, the relative abundance of beneficial bacteria (e.g., Lactobacillus and Bifidobacterium) increased, whereas that of potentially pathogenic taxa (e.g., Escherichia-Shigella and Bilophila) decreased. Furthermore, P. cocos polysaccharides enhanced the production of key microbial metabolites, significantly upregulating compounds such as L-cystine and etelcalcetide. Collectively, these findings underscore the beneficial role of P. cocos polysaccharides in promoting intestinal health and highlight their potential as prebiotics in the functional food industry.","PeriodicalId":19367,"journal":{"name":"NPJ Science of Food","volume":"9 1","pages":"53"},"PeriodicalIF":6.3,"publicationDate":"2025-04-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12015419/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144029916","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effects of RBX oleogel and heat-moisture-treated rice flour in food matrices on digestibility and microbiota. RBX油凝胶和热湿处理米粉对食品基质消化率和微生物群的影响。

IF 6.3 1区农林科学

NPJ Science of Food Pub Date : 2025-04-22 DOI: 10.1038/s41538-025-00418-7

Tidarat Norsuwan, Thanutchaporn Kumrungsee, Noriyuki Yanaka, Tomoka Nagao, Masubon Thongngam

{"title":"Effects of RBX oleogel and heat-moisture-treated rice flour in food matrices on digestibility and microbiota.","authors":"Tidarat Norsuwan, Thanutchaporn Kumrungsee, Noriyuki Yanaka, Tomoka Nagao, Masubon Thongngam","doi":"10.1038/s41538-025-00418-7","DOIUrl":"https://doi.org/10.1038/s41538-025-00418-7","url":null,"abstract":"Recently, oleogel and heat-moisture-treated (HMT) modified starch have gained much attention as a potential margarine replacement and a low-digestible starch, respectively. To date, most studies have investigated oleogel and HMT starch as individual components, while information regarding their physiological properties as a food matrix form is scarce. Here, we demonstrated that the HMT starch-oleogel food matrix exhibited the lowest plasma lipid and glucose levels, but high lipid and fecal excretion in mice, indicating that the food matrix possibly lowered lipid and carbohydrate digestibility. The resistant starch (RS) content was markedly decreased in the food matrix, suggesting other factors, such as lipid barriers and gel viscosity, in lowering the food-matrix digestibility. Roseburia, Adlercreutzia, and rc4-4 were enriched, while Bifidobacterium and Clostridium were reduced in the food matrix group. The present study provides insights into the in vivo physiological properties and the health benefits of oleogel and HMT starch in food matrix forms.","PeriodicalId":19367,"journal":{"name":"NPJ Science of Food","volume":"9 1","pages":"54"},"PeriodicalIF":6.3,"publicationDate":"2025-04-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12015495/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144032097","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A unique spontaneously immortalised cell line from pig with enhanced adipogenic capacity. 一种独特的自发永生化的猪细胞系，具有增强的脂肪生成能力。

IF 6.3 1区农林科学

NPJ Science of Food Pub Date : 2025-04-20 DOI: 10.1038/s41538-025-00413-y

Thomas Thrower, Susanna E Riley, Seungmee Lee, Cristina L Esteves, F Xavier Donadeu

{"title":"A unique spontaneously immortalised cell line from pig with enhanced adipogenic capacity.","authors":"Thomas Thrower, Susanna E Riley, Seungmee Lee, Cristina L Esteves, F Xavier Donadeu","doi":"10.1038/s41538-025-00413-y","DOIUrl":"https://doi.org/10.1038/s41538-025-00413-y","url":null,"abstract":"Cultivated meat promises to address some of the pressing challenges associated with large-scale production of animals for food. An important limitation to realising such promise is the lack of readily available cell lines that can be expanded robustly for scale-up culture while maintaining the capacity to differentiate into tissues of interest, namely fat and muscle. Here, we report a porcine mesenchymal stem cell line (FaTTy) which, uniquely, upon spontaneously immortalisation acquired enhanced adipogenic efficiency, close to 100%, that has now been maintained for over 200 population doublings. FaTTy is able to differentiate with high efficiency in both 2D and 3D contexts and produces mature adipocytes upon prolonged differentiation. Moreover, FaTTy adipocytes display fatty acid profiles largely similar to native pig fat but with higher monounsaturated-to-saturated ratios. FaTTy displays minor aneuploidy, characterised by lack of Y chromosome, and lacks typical genetic or functional properties of tumorigenic cells. These highly distinctive characteristics, together with its non-genetically modified nature, make FaTTy a very attractive, potentially game-changing resource for food manufacturing, and particularly cultivated meat.","PeriodicalId":19367,"journal":{"name":"NPJ Science of Food","volume":"9 1","pages":"52"},"PeriodicalIF":6.3,"publicationDate":"2025-04-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12010005/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143991809","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Advancements in delivery systems for dietary polyphenols in enhancing radioprotection effects: challenges and opportunities. 膳食多酚输送系统在增强辐射防护效果方面的进展：挑战与机遇。

IF 6.3 1区农林科学

NPJ Science of Food Pub Date : 2025-04-14 DOI: 10.1038/s41538-025-00419-6

Yuxuan Lu, Kai Wang, Lin Hu

{"title":"Advancements in delivery systems for dietary polyphenols in enhancing radioprotection effects: challenges and opportunities.","authors":"Yuxuan Lu, Kai Wang, Lin Hu","doi":"10.1038/s41538-025-00419-6","DOIUrl":"https://doi.org/10.1038/s41538-025-00419-6","url":null,"abstract":"Radiotherapy, a widely employed cancer treatment, often triggers diverse inflammatory responses such as radiation enteritis, pulmonary injury, pelvic inflammation, dermatitis, and osteitis. Dietary polyphenols have recently emerged as promising agents for mitigating radiation-induced inflammation. However, their clinical application faced challenges related to variable bioavailability, individual pharmacokinetics, optimal dosing, and limited clinical evidence. Current researches revealed the efficacy of bioactive small molecule polyphenols in addressing radiation-induced inflammation. In this review, along with a comprehensive examination of the etiology and categories of radiation-induced inflammatory conditions, the diversity of polyphenols and elucidating their anti-inflammatory mechanisms are explored. This study emphasizes the recent progresses in delivery systems for dietary polyphenols, aiming to enhance radioprotection effects. The optimized utilization of polyphenols, with a theoretical framework and reference guide, is of paramount relevance. Through diverse delivery mechanisms, the more effective and safer radioprotective strategies become achievable. This endeavor aspires to contribute to breakthroughs in the dietary polyphenols' application, significantly enhancing human health protection during radiotherapy. These comprehensive insights presented here also support (pre)-clinical practices in navigating the complexities of utilizing dietary polyphenols for radioprotection, fostering advancements in the field and improving patient outcomes.","PeriodicalId":19367,"journal":{"name":"NPJ Science of Food","volume":"9 1","pages":"51"},"PeriodicalIF":6.3,"publicationDate":"2025-04-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11997175/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144029903","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Food culture and cell-culture: technical, ethical and social frontiers. 食物培养和细胞培养：技术、伦理和社会前沿。

IF 6.3 1区农林科学

NPJ Science of Food Pub Date : 2025-04-11 DOI: 10.1038/s41538-025-00417-8

Sghaier Chriki, William Hallman, Jean-François Hocquette, Marie-Pierre Ellies-Oury, Masami Takeuchi

引用次数: 0