NPJ Vaccines最新文献_第4页

DNA vaccine targeting betacoronavirus spike protein blocks neuroinvasion and neuroinflammation in swine via dual antiviral-immunomodulatory action. 靶向乙型冠状病毒刺突蛋白的DNA疫苗通过抗病毒-免疫双重调节作用阻断猪的神经侵袭和神经炎症。

IF 6.5 1区医学

NPJ Vaccines Pub Date : 2025-08-07 DOI: 10.1038/s41541-025-01247-1

Huabo Yu, Junchao Shi, Guoce Fu, Zezhao Cao, Ruizhao Qiu, Tianqi Zhao, Jing Zhang, Yungang Lan, Jiyu Guan, Kui Zhao, Feng Gao, Wenqi He, Zi Li

{"title":"DNA vaccine targeting betacoronavirus spike protein blocks neuroinvasion and neuroinflammation in swine via dual antiviral-immunomodulatory action.","authors":"Huabo Yu, Junchao Shi, Guoce Fu, Zezhao Cao, Ruizhao Qiu, Tianqi Zhao, Jing Zhang, Yungang Lan, Jiyu Guan, Kui Zhao, Feng Gao, Wenqi He, Zi Li","doi":"10.1038/s41541-025-01247-1","DOIUrl":"10.1038/s41541-025-01247-1","url":null,"abstract":"Porcine hemagglutinating encephalomyelitis virus (PHEV), a neurotropic betacoronavirus, causes fatal neurological disease in piglets, yet no licensed vaccines exist. Here, we developed a DNA vaccine encoding the receptor-binding domain (RBD) of PHEV spike protein fused to IgG1 Fc, adjuvanted with GEL01 (RBD + GEL01). Immunization in mice and piglets elicited robust neutralizing antibodies (titers up to 1:446 and 1:147, respectively) and Th1-biased cellular immunity. The vaccine restricted viral neuroinvasion, reducing brain viral loads by >90% and confining PHEV to discrete olfactory and cortical regions. Vaccinated animals exhibited preserved motor coordination, cognitive function, and minimal neuropathology. Transcriptomic analysis revealed suppression of proinflammatory mediators (e.g., Cxcl2, Saa3) and enhanced neural repair pathways, highlighting dual virological control and immunomodulatory mechanisms. As the first DNA vaccine against PHEV, the RBD + GEL01 candidate offers scalable protection against neurotropic coronaviruses by dual antiviral-immunomodulatory strategy, underscoring its potential to mitigate economic and zoonotic risks.","PeriodicalId":19335,"journal":{"name":"NPJ Vaccines","volume":"10 1","pages":"187"},"PeriodicalIF":6.5,"publicationDate":"2025-08-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12331951/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144799757","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Evaluation of highly conserved Burkholderia pseudomallei outer membrane proteins as protective antigens against respiratory melioidosis. 高度保守的假马利氏伯克氏菌外膜蛋白作为呼吸道类鼻疽病保护抗原的评价。

IF 6.5 1区医学

NPJ Vaccines Pub Date : 2025-08-06 DOI: 10.1038/s41541-025-01246-2

Alexander J Badten, Susana Oaxaca-Torres, Ritwika S Basu, Matthieu G Gagnon, Alfredo G Torres

{"title":"Evaluation of highly conserved Burkholderia pseudomallei outer membrane proteins as protective antigens against respiratory melioidosis.","authors":"Alexander J Badten, Susana Oaxaca-Torres, Ritwika S Basu, Matthieu G Gagnon, Alfredo G Torres","doi":"10.1038/s41541-025-01246-2","DOIUrl":"10.1038/s41541-025-01246-2","url":null,"abstract":"Burkholderia pseudomallei (Bpm), the etiological agent of melioidosis, lacks approved vaccines. However, several candidates have demonstrated protection in animal models. Interestingly, some of these vaccines can induce cross-protective immunity against the closely related species B. mallei. This led us to search the Bpm proteome for antigens that are highly conserved in more distantly related pathogenic Burkholderia species, which could potentially serve as components of a pan-Burkholderia vaccine. We identified three proteins, OmpA1, OmpA2, and Pal, which were coupled to an immunogenic gold nanoparticle (AuNP) platform. Intranasal immunization with these vaccines resulted in the induction of robust Th1/Th2-balanced responses and mucosal immunity, and the AuNP-OmpA1 and AuNP-OmpA2 vaccinated animals were significantly protected from a lethal Bpm respiratory challenge. Serum antibodies were highly cross-reactive to B. mallei and partially cross-reactive to B. multivorans and B. cenocepacia, indicating that the antigens contain highly conserved epitopes that can be incorporated in a pan-Burkholderia vaccine.","PeriodicalId":19335,"journal":{"name":"NPJ Vaccines","volume":"10 1","pages":"186"},"PeriodicalIF":6.5,"publicationDate":"2025-08-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12328632/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144794977","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Priming VRC01-precursor B cells with non-envelope immunogens disfavors boosting with HIV-1 envelope. 用非包膜免疫原引发vrc01前体B细胞不利于HIV-1包膜增强。

IF 6.5 1区医学

NPJ Vaccines Pub Date : 2025-08-05 DOI: 10.1038/s41541-025-01235-5

Andrew Wilcox-King, Yu-Hsin Wan, Samuel C Scharffenberger, Crystal B Chhan, Amelia R Davis, Leah J Homad, Emilie Seydoux, Kellie J MacPhee, Latha Kallur Siddaramaiah, Mariane Melo, Pia Dosenovic, Darrell J Irvine, Ollivier Hyrien, Leonidas Stamatatos, Andrew T McGuire

{"title":"Priming VRC01-precursor B cells with non-envelope immunogens disfavors boosting with HIV-1 envelope.","authors":"Andrew Wilcox-King, Yu-Hsin Wan, Samuel C Scharffenberger, Crystal B Chhan, Amelia R Davis, Leah J Homad, Emilie Seydoux, Kellie J MacPhee, Latha Kallur Siddaramaiah, Mariane Melo, Pia Dosenovic, Darrell J Irvine, Ollivier Hyrien, Leonidas Stamatatos, Andrew T McGuire","doi":"10.1038/s41541-025-01235-5","DOIUrl":"10.1038/s41541-025-01235-5","url":null,"abstract":"VRC01-class antibodies are a genetically restricted class of antibodies capable of potently neutralizing diverse strains of HIV-1. Unmutated VRC01 precursors fail to recognize recombinant HIV-1 Envelope (Env) proteins, which necessitated the development of germline targeting vaccine immunogens capable of initiating VRC01-class B cell response. Among these, we developed an anti-idiotypic monoclonal antibody (ai-mAb)-derived VRC01 class targeting immunogen. Because it is distinct from Env, we speculated that the ai-mAb will selectively engage naive VRC01 class B cells while limiting B cell responses directed at off-target epitopes on Env during prime-boost regimens. Here, we evaluated the serum and B cell responses to ai-mAb prime/Env boost, and Env-prime/Env boost regimens in a murine adoptive transfer model where VRC01 precursor B cells are present at physiological levels. We found that the Env-Env regimen led to the greatest expansion of on-target VRC01 B cells, drove larger VRC01-class GC responses, and elicited higher titers of circulating antibodies despite also eliciting substantial off-target Env-specific responses. Single-cell sorting experiments revealed that the ai-mAb was driving off-track somatic mutations. IgG transfer experiments demonstrated that circulating off-target antibodies provide a positive feedback mechanism that potentiates on-target B cell responses. Collectively, the results suggest that non-Env immunogens are not ideal for priming VRC01-class B cells, where sequential boosting with Env will be required to drive maturation of neutralizing breadth.","PeriodicalId":19335,"journal":{"name":"NPJ Vaccines","volume":"10 1","pages":"185"},"PeriodicalIF":6.5,"publicationDate":"2025-08-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12325944/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144789629","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Matrix-M adjuvant triggers inflammasome activation and enables antigen cross-presentation through induction of lysosomal membrane permeabilization. 基质- m佐剂触发炎性小体活化，并通过诱导溶酶体膜渗透使抗原交叉递呈。

IF 6.5 1区医学

NPJ Vaccines Pub Date : 2025-08-05 DOI: 10.1038/s41541-025-01243-5

Behdad Zarnegar, Berit Carow, Jens Eriksson, Eva Spennare, Pontus Öhlund, Eray Akpinar, Emelie Bringeland, Ingrid Lekberg Osterman, Lena Lundqvist, Johanna Antti, Niklas Handin, Per-Henrik Helgesson, Johan Bankefors, Karin Lövgren Bengtsson, Mikael E Sellin, Anna-Karin E Palm, Linda Stertman, Carolina Lunderius Andersson

{"title":"Matrix-M adjuvant triggers inflammasome activation and enables antigen cross-presentation through induction of lysosomal membrane permeabilization.","authors":"Behdad Zarnegar, Berit Carow, Jens Eriksson, Eva Spennare, Pontus Öhlund, Eray Akpinar, Emelie Bringeland, Ingrid Lekberg Osterman, Lena Lundqvist, Johanna Antti, Niklas Handin, Per-Henrik Helgesson, Johan Bankefors, Karin Lövgren Bengtsson, Mikael E Sellin, Anna-Karin E Palm, Linda Stertman, Carolina Lunderius Andersson","doi":"10.1038/s41541-025-01243-5","DOIUrl":"10.1038/s41541-025-01243-5","url":null,"abstract":"Matrix-M® adjuvant, containing saponins, delivers a potent adjuvant effect and good safety profile. Given that Matrix-M is composed of Matrix-A and Matrix-C particles, comprising different saponin fractions, understanding their distinct roles can provide deeper insight into the mechanism of action of Matrix-M and guide future applications. Here, we demonstrate that the antigen and Matrix-M, Matrix-A, or Matrix-C colocalize in lysosomes following uptake by bone marrow-derived dendritic cells. Matrix-M, Matrix-A, and Matrix-C induce lysosomal membrane permeabilization (LMP), but Matrix-C shows the highest LMP potential. LMP is required for interleukin (IL)-1β and IL-18 secretion in vitro. In vivo, a robust adjuvant effect of Matrix-M, Matrix-A, and Matrix-C is observed, both in the presence and absence of the NLRP3 inflammasome. LMP induced by Matrix-M, as well as Matrix-A and Matrix-C, also enables antigen cross-presentation. Thus, Matrix-induced LMP explains the capability of Matrix-M-adjuvanted protein vaccines to induce CD8+ T-cell responses.","PeriodicalId":19335,"journal":{"name":"NPJ Vaccines","volume":"10 1","pages":"184"},"PeriodicalIF":6.5,"publicationDate":"2025-08-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12325594/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144789628","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Overview of vaccines for adults authorized, recommended, and implemented in the European Union. 欧盟批准、推荐和实施的成人疫苗概述

IF 6.5 1区医学

NPJ Vaccines Pub Date : 2025-08-04 DOI: 10.1038/s41541-025-01242-6

Jade Pattyn, Odile Launay, Robert Steffen, Birgit Weinberger, Giovanni Gabutti, Alojz Ihan, Thomas Weinke, Ligita Jancoriene, Paolo Bonanni, Pierre Van Damme

引用次数: 0

Elevated alanine transaminase in liver transplant recipients after BNT162b2 vaccination: a cohort study. 接种BNT162b2疫苗后肝移植受者丙氨酸转氨酶升高：一项队列研究

IF 6.5 1区医学

NPJ Vaccines Pub Date : 2025-08-02 DOI: 10.1038/s41541-025-01233-7

Jacob Siewertsen Bergmann, Sebastian Rask Hamm, Louise Bering, Christian Ross Pedersen, Ask Bock, Safura-Luise Heidari, Gerda Elisabeth Villadsen, Annette Dam Fialla, Gro Linno Willemoe, Peter Holland-Fischer, Susanne Dam Nielsen

{"title":"Elevated alanine transaminase in liver transplant recipients after BNT162b2 vaccination: a cohort study.","authors":"Jacob Siewertsen Bergmann, Sebastian Rask Hamm, Louise Bering, Christian Ross Pedersen, Ask Bock, Safura-Luise Heidari, Gerda Elisabeth Villadsen, Annette Dam Fialla, Gro Linno Willemoe, Peter Holland-Fischer, Susanne Dam Nielsen","doi":"10.1038/s41541-025-01233-7","DOIUrl":"10.1038/s41541-025-01233-7","url":null,"abstract":"Liver transplant (LTx) recipients risk severe COVID-19. Vaccination reduces this risk. However, there may be side effects, including elevated alanine transaminase (ALT) which could lead to increased use of liver biopsy. We aimed to describe prevalence and relative incidence of elevated ALT 90 days before and after BNT162b2 vaccination in LTx recipients. Furthermore, we aimed to describe changes in prevalence of liver biopsies before and after BNT162b2 vaccination. We included 393 LTx recipients from The Danish Comorbidity in Liver Transplant Recipients (DACOLT) study. We calculated prevalence of elevated ALT and liver biopsies before and after each BNT162b2 vaccine dose. We used self-control case series (SCCS) analysis to investigate whether vaccination was associated with higher relative incidence of elevated ALT. Prevalence of elevated ALT, around each vaccine dose, was comparable. We did not find higher relative incidence of elevated ALT after vaccination. The prevalence of liver biopsies around vaccination was comparable.","PeriodicalId":19335,"journal":{"name":"NPJ Vaccines","volume":"10 1","pages":"181"},"PeriodicalIF":6.5,"publicationDate":"2025-08-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12317978/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144768825","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Impact of COVID-19 vaccination coverage on global disability burden of Guillain-Barré syndrome. COVID-19疫苗接种覆盖率对格林-巴- <s:1>综合征全球残疾负担的影响

IF 6.5 1区医学

NPJ Vaccines Pub Date : 2025-08-02 DOI: 10.1038/s41541-025-01239-1

Xīn Gào, Chen Zhao, Junting Yang, Ziming Yang, Jingnan Feng, Siyan Zhan, Dongsheng Fan, Zhike Liu

引用次数: 0

Modulation of oral vaccine efficacy by the gut microbiota. 肠道菌群对口服疫苗效力的调节。

IF 6.5 1区医学

NPJ Vaccines Pub Date : 2025-08-01 DOI: 10.1038/s41541-025-01240-8

Yingying Hou, Jinqi Li, Yue Wu

引用次数: 0

How Rwanda mounted a research response with an investigational vaccine just ten days into a Marburg outbreak. 卢旺达是如何在马尔堡疫情爆发10天后，用一种研究性疫苗开展研究应对的。

IF 6.5 1区医学

NPJ Vaccines Pub Date : 2025-08-01 DOI: 10.1038/s41541-025-01224-8

Sabin Nsanzimana, Noella Bigirimana, Richard Hatchett, Sue Bailey, Natacha Butera, Yvan Butera, Jakob P Cramer, Amy Finan, Caroline M Forkin, Adam M Hacker, Sibomana Hassan, Vicky Leamy, Vincent Mutabazi, Julien Nyombayire, Eric Remera, Edson Rwagasore, Eduardo Tedeschi, Kelly L Warfield, Nicole Lurie

引用次数: 0

Vaccination-induced neutralizing antibodies in immunocompetent hosts correlate with protection against rickettsiae. 免疫能力强的宿主中疫苗诱导的中和抗体与对立克次体的保护相关。

IF 6.5 1区医学

NPJ Vaccines Pub Date : 2025-08-01 DOI: 10.1038/s41541-025-01228-4

Loka Reddy Velatooru, Jessica Plante, Chen Yi Chu, Garrett Cutchin, Bing Zhu, Nicole Burkhardt, Carsen Roach, Yingzi Cong, Shahid Karim, Yong-Fang Kuo, David H Walker, Ulrike Munderloh, Rong Fang

{"title":"Vaccination-induced neutralizing antibodies in immunocompetent hosts correlate with protection against rickettsiae.","authors":"Loka Reddy Velatooru, Jessica Plante, Chen Yi Chu, Garrett Cutchin, Bing Zhu, Nicole Burkhardt, Carsen Roach, Yingzi Cong, Shahid Karim, Yong-Fang Kuo, David H Walker, Ulrike Munderloh, Rong Fang","doi":"10.1038/s41541-025-01228-4","DOIUrl":"10.1038/s41541-025-01228-4","url":null,"abstract":"We previously demonstrated that a single-dose immunization with a live-attenuated Rickettsia parkeri mutant 3A2 confers complete protection against murine spotted fever rickettsioses. In this study, we investigated whether vaccination-elicited serum antibodies serve as immune correlates of protection against rickettsiae. Immunization of immunocompetent C3H/HeN mice with 3A2 induced a robust and durable level of IgG antibody response, predominantly comprising IgG2a, IgG3, and IgG2b subclasses, and was associated with a significant expansion of CD19+ CD45R+ IgDlow plasma cells. Compared to mock controls, passive transfer of immune sera from 3A2-immunized mice protected C3H-SCID mice from R. parkeri challenge via multiple inoculation routes, including i.v., i.d. and i.d. plus tick saliva. Strikingly, serum antibodies of 3A2-immunized mice significantly reduced the number of R. parkeri plaques in vitro, indicating direct neutralizing activity. Collectively, our findings demonstrate that vaccination-induced serum IgG antibodies correlate with protection against rickettsial infection and possess direct neutralizing effects on rickettsiae.","PeriodicalId":19335,"journal":{"name":"NPJ Vaccines","volume":"10 1","pages":"180"},"PeriodicalIF":6.5,"publicationDate":"2025-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12316887/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144765113","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0