NFS JournalPub Date : 2025-05-06DOI: 10.1016/j.nfs.2025.100228
Anastassia Zeinatulina , Aleksei Kaleda , Maria Kuhtinskaja , Tiina Kriščiunaite
{"title":"Optimisation of extraction method for quantification of free fatty acids in oat-, pea- and faba-bean-based protein sources by liquid chromatography coupled with mass spectrometry","authors":"Anastassia Zeinatulina , Aleksei Kaleda , Maria Kuhtinskaja , Tiina Kriščiunaite","doi":"10.1016/j.nfs.2025.100228","DOIUrl":"10.1016/j.nfs.2025.100228","url":null,"abstract":"<div><div>Plant-based protein sources, including oat, pea, and faba bean, are widely used in milk and meat alternatives. However, these crops can be inherently bitter, which limits their use in some food products. Processing into flours, protein concentrates, and isolates may cause changes in chemical composition and influence bitterness. We present an optimised and validated LC-MS method using isopropanol:methanol (1:1, <em>v</em>/v) solvent extraction to determine selected bitter-tasting free fatty acids (FFAs). Accurate quantification of FFAs (linolenic, myristic, palmitic, linoleic, oleic, and stearic acids) was achieved using isotopically labelled oat flour extract as an internal standard. The total FFA content in whole grain oat flour, oat protein concentrate, pea flour and protein isolate, and faba bean flour and protein isolate ranged from 4.4 to 3841 mg/100 g dry weight. FFA analysis aids in assessing bitterness and selecting processing techniques to reduce these compounds in plant-based foods.</div></div>","PeriodicalId":19294,"journal":{"name":"NFS Journal","volume":"39 ","pages":"Article 100228"},"PeriodicalIF":4.1,"publicationDate":"2025-05-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143947311","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Encapsulation of mango peel bioactive compounds in milk, gum acacia, and maltodextrin improves its stability","authors":"Mahbuba Rahman Tripty, Asmaul Husna Nupur, Jannatul Ferdushi Jany, Maria Afroz Toma, Md. Anisur Rahman Mazumder","doi":"10.1016/j.nfs.2025.100227","DOIUrl":"10.1016/j.nfs.2025.100227","url":null,"abstract":"<div><div>There is an increased interest and need to make our economy more circular and our diets healthier and more sustainable. Mango peel is often discarded as waste after consuming mango pulp, despite its excellent source of bioactive compounds, especially phenolic compounds. The aim of this study was to develop encapsulated milk powder containing mango peel polyphenol (MPP) and assess the effects of MPP on the physicochemical properties, antioxidant activity, sensory and storage stability of milk powder. MPP was encapsulated with milk, gum acacia (4, 6 and 8 % <em>w</em><em>/v</em>), and maltodextrin (10 % <em>w/v</em>) using spray drying and cabinet drying. Nutritional analysis revealed that ripe mango peel powder provide total polyphenol of 64.37 ± 0.26 mg GAE/g, total flavonoid of 10.73 ± 0.11 (mg QuE/g), and antioxidant activity (DPPH) of 52.56 %. Regarding the physico-chemical characteristics of the MPP encapsulated spray-dried milk powder showed increases (<em>p</em> < 0.05) in bulk density, flowability, cohesiveness, hygroscopicity, and solubility, whereas cabinet-dried milk powder showed increases (<em>p</em> < 0.05) in moisture content and tapped density. Spray-dried milk powder had greater retention (p < 0.05) of color parameters and retained high phenolic compounds during storage at 4 or 25 °C for 60 days compared to cabinet-dried milk powder. No significant differences (<em>p</em> > 0.05) were observed between 4 % and 6 % <em>w</em>/<em>v</em> gum acacia concentrations in terms of encapsulation efficiency, physico-chemical properties and color retention. The results show that MPP polyphenol can be encapsulated by combining gum acacia (4 or 6 %), 10 % maltodextrin, and milk powder at 4 °C for longer shelf life.</div></div>","PeriodicalId":19294,"journal":{"name":"NFS Journal","volume":"39 ","pages":"Article 100227"},"PeriodicalIF":4.1,"publicationDate":"2025-04-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143902245","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
NFS JournalPub Date : 2025-03-01DOI: 10.1016/j.nfs.2025.100221
Junhyo Cho , Eun-Ho Lee , Young-Je Cho , Yeonhwa Park
{"title":"Jatrorrhizine, an alkaloid isolated from Phellodendron amurense, reduces melanogenesis in mouse B16F10 melanoma cells","authors":"Junhyo Cho , Eun-Ho Lee , Young-Je Cho , Yeonhwa Park","doi":"10.1016/j.nfs.2025.100221","DOIUrl":"10.1016/j.nfs.2025.100221","url":null,"abstract":"<div><div>Jatrorrhizine isolated from <em>Phellodendron amurense</em> has been reported as one of the major phenolic alkaloid compounds. Previous studies indicated that jatrorrhizine has potential health benefits. In this study, we used jatrorrhizine isolated from <em>P. amurense</em> to determine its effects on melanogenesis using B16F10 mouse melanoma cells. Jatrorrhizine at 20–100 μM inhibited α-melanocyte-stimulating hormone-induced melanin biosynthesis without cytotoxicity. Treatment with jatrorrhizine inhibited melanocortin 1 receptor, increased transforming growth factor-β1, and inhibited microphthalmia transcription factor, tyrosinase-related protein-1, tyrosinase-related protein-2, tyrosinase expression, and tyrosinase activity compared to the α-MSH-treated control. In addition, treatment with jatrorrhizine inhibited <em>RAB27A</em> and <em>MYO5A</em>, which are key components of melanosome transport. The current results suggest that jatrorrhizine has the potential to be used in functional cosmetics and beauty foods to improve skin tone. Further <em>in vivo</em> studies using jatrorrhizine would be needed to support the current observations.</div></div>","PeriodicalId":19294,"journal":{"name":"NFS Journal","volume":"38 ","pages":"Article 100221"},"PeriodicalIF":4.1,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143561885","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
NFS JournalPub Date : 2025-03-01DOI: 10.1016/j.nfs.2025.100222
Masayuki Yamaga , Masakatsu Nohara , Akihisa Hata , Takashi Ito , Kayo Furumoto , Naomi Ohta , Jiro Miyamae , Hiroko Tani , Ayanori Yamaki , Noboru Fujitani
{"title":"Tissue distribution and accumulation of cinnamic acid derivatives from Brazilian green propolis in mice","authors":"Masayuki Yamaga , Masakatsu Nohara , Akihisa Hata , Takashi Ito , Kayo Furumoto , Naomi Ohta , Jiro Miyamae , Hiroko Tani , Ayanori Yamaki , Noboru Fujitani","doi":"10.1016/j.nfs.2025.100222","DOIUrl":"10.1016/j.nfs.2025.100222","url":null,"abstract":"<div><div>Cinnamic acid derivatives, particularly prenylated derivatives, are characteristic components of Brazilian green propolis (BGP) and have been attracting attention for their health benefits. However, few reports have investigated their tissue distribution. This study aimed to elucidate the tissue distribution of cinnamic acid derivatives and examine the effects of prenylation by administering BGP to mice. The distribution level of <em>p</em>-coumaric acid was higher in the kidneys than in the liver or intestine. Artepillin C was detected at higher levels in the liver and intestine than in the kidney, and was also distributed in the brain, muscle, spleen, pancreas, and adipose tissue. These results suggested that prenylation enhanced the tissue uptake of cinnamic acid derivatives, which may facilitate the understanding of their functional mechanisms and promote their use in pharmaceuticals and functional foods.</div></div>","PeriodicalId":19294,"journal":{"name":"NFS Journal","volume":"38 ","pages":"Article 100222"},"PeriodicalIF":4.1,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143601374","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
NFS JournalPub Date : 2025-03-01DOI: 10.1016/j.nfs.2025.100223
Patryk Adamski, Arkadiusz Zakrzewski, Patryk Wiśniewski, Wioleta Chajęcka-Wierzchowska, Anna Zadernowska
{"title":"High-pressure processing (HPP) alters tetracycline resistance in Listeria monocytogenes: A phenotypic and genotypic study","authors":"Patryk Adamski, Arkadiusz Zakrzewski, Patryk Wiśniewski, Wioleta Chajęcka-Wierzchowska, Anna Zadernowska","doi":"10.1016/j.nfs.2025.100223","DOIUrl":"10.1016/j.nfs.2025.100223","url":null,"abstract":"<div><div>This study insights into the effects of high-pressure processing (HPP) on the phenotypic resistance and expression of tetracycline resistance genes (<em>tetA</em>_1, <em>tetA</em>_2, <em>tetA</em>_3, <em>tetC</em>, <em>tetR</em>) in <em>Listeria monocytogenes</em> strains. A total of 29 strains were tested for minimum inhibitory concentration values (MIC) of tetracycline before and after HPP treatment (200 MPa/5 min). Results showed a significant increase in MIC values post-treatment, with 7 % of the strains exhibiting resistance. Gene expression analysis revealed overexpression of <em>tetA</em>_1 and <em>tetA</em>_2 genes, while <em>tetA</em>_3, <em>tetC</em>, and <em>tetR</em> showed varied responses, suggesting a strain specific expression pattern. These findings indicate that HPP induced stress may influence the development of antibiotic resistance in L. <em>monocytogenes</em>, highlighting the importance of monitoring resistance genes in food safety protocols.</div></div>","PeriodicalId":19294,"journal":{"name":"NFS Journal","volume":"38 ","pages":"Article 100223"},"PeriodicalIF":4.1,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143609249","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
NFS JournalPub Date : 2025-02-04DOI: 10.1016/j.nfs.2025.100220
María Paula Polo Muñoz, Remigio Yamid Pismag Portilla, Jesús Eduardo Bravo Gomez, José Fernando Solanilla Duque, Diego Fernando Roa Acosta
{"title":"Instant quinoa powder: Effect of enzymatic hydrolysis and extrusion on its physicochemical and rheological properties","authors":"María Paula Polo Muñoz, Remigio Yamid Pismag Portilla, Jesús Eduardo Bravo Gomez, José Fernando Solanilla Duque, Diego Fernando Roa Acosta","doi":"10.1016/j.nfs.2025.100220","DOIUrl":"10.1016/j.nfs.2025.100220","url":null,"abstract":"<div><div>The combined effects of extrusion and enzymatic hydrolysis with a neutral protease (endoprotease) on quinoa germ flour have been studied in this work. A 2 × 3 factorial design with two temperature levels (60 °C - 90 °C - 100 °C and 60 °C - 100 °C - 120 °C) and three protease concentrations (0 %<em>w</em>/<em>v</em>, 0.6 %w/v, 1.2 %<em>w</em>/<em>v</em>). The hydrolyzed quinoa germ flours were characterized in terms of their physicochemical and rheological properties, antioxidant capacity by ABTS+, in-vitro digestibility of carbohydrates and protein, infrared spectroscopy, and their rheological behavior in aqueous dispersion at 12 % (<em>w</em>/w). The results showed that enzymatic hydrolysis and extrusion temperature influenced hydrolyzed quinoa germ flours properties. The highest protein digestion (65 mg leucine/g protein) was obtained with 0.6 % protease and 100 °C extrusion. Antioxidant activity increased with protease concentration but decreased with temperature. The solubility of hydrolyzed quinoa germ flours improved after enzymatic hydrolysis, while water absorption decreased. The treatment with 0.6 % protease and 100 °C extrusion produced high amounts of slowly digestible starch and RS, and enhanced protein digestibility compared to the other treatments. Infrared spectroscopy revealed changes in amide functional groups A, B, and I due to hydrolysis. The modification of these flours through enzymatic hydrolysis and extrusion after the tecno-functional properties. This research highlights the importance of understanding the interactions between different sources of proteins and how they contribute to the overall characteristics of the final product.</div></div>","PeriodicalId":19294,"journal":{"name":"NFS Journal","volume":"38 ","pages":"Article 100220"},"PeriodicalIF":4.1,"publicationDate":"2025-02-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143395806","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
NFS JournalPub Date : 2025-02-01DOI: 10.1016/j.nfs.2025.100218
Eszter Benes , Attila Gere
{"title":"Multicriteria decision making-based approach to classify loose-leaf teas","authors":"Eszter Benes , Attila Gere","doi":"10.1016/j.nfs.2025.100218","DOIUrl":"10.1016/j.nfs.2025.100218","url":null,"abstract":"<div><div>Near infrared spectra of 75 different loose-leaf teas were analyzed based on their oxidational state: white, green, matcha, oolong, black, dark and pu-erh. Different spectral transformations (MSC, SNV and derivatives) and seven supervised linear and non-linear chemometric methods were performed. Classification methods were ranked based on their model performance metrics. In the ranking of the models, multicriteria decision making (MCDM) methods have crucial role, of which sum of ranking differences (SRD) method was used. SNV preprocessing showed better performance compared to MSC and FD + SNV. Among the models, linear support vector machine (lSVM) gave satisfactory performance regardless of the preprocessing. lSVM used on SNV preprocessed data proved to be the far best model, with 83.3 % accuracy. However, it is important to note that there are no general rules regarding model performances and proper testing is always advised. For such, multicriteria decision making models (and especially SRD) is strongly advised.</div></div>","PeriodicalId":19294,"journal":{"name":"NFS Journal","volume":"38 ","pages":"Article 100218"},"PeriodicalIF":4.1,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143172499","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
NFS JournalPub Date : 2025-01-31DOI: 10.1016/j.nfs.2025.100219
Jonas Pospiech, Johanita Kruger, Jan Frank
{"title":"Plant-based burger patties are a source of bioaccessible iron and zinc","authors":"Jonas Pospiech, Johanita Kruger, Jan Frank","doi":"10.1016/j.nfs.2025.100219","DOIUrl":"10.1016/j.nfs.2025.100219","url":null,"abstract":"<div><h3>Background</h3><div>The consumption of plant-based meat alternatives has increased substantially in recent years. However, due to the presence of absorption inhibitors, such as phytate, oxalate, and condensed tannins, the uptake of zinc and iron from plant foods may be low. We therefore quantified minerals (Fe, Zn), inhibitors (phytate, oxalate, condensed tannins), and enhancers (ascorbic acid, carotenoids) of mineral absorption in pea protein-, soy protein-, soy flour-, and sunflower protein-based burger patties and the resulting bioaccessibility of the contained iron and zinc conducting <em>in vitro</em> digestion experiments.</div></div><div><h3>Results</h3><div>The soy flour-based burger had nearly complete bioaccessibility for zinc (105 ± 6 %) and iron (101 ± 11 %), while the three other plant burgers had significantly lower bioaccessibilities (zinc, 9–12 %; iron, 43–57 %). The phytate but not the oxalate and condensed tannin contents of the patties were negatively correlated with the bioaccessibility of iron and zinc (<em>p</em> < 0.05).</div></div><div><h3>Conclusion</h3><div>Plant-based meat alternatives may be an important source of bioaccessible iron and zinc, if they contain low amounts of phytate, and may contribute to an adequate supply of these minerals.</div></div>","PeriodicalId":19294,"journal":{"name":"NFS Journal","volume":"38 ","pages":"Article 100219"},"PeriodicalIF":4.1,"publicationDate":"2025-01-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143377906","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Potential plant proteins for functional food ingredients: Composition, utilization and its challenges","authors":"Paul Ndubuisi Anyiam , Suphat Phongthai , Lutz Grossmann , Young Hoon Jung , Samart Sai-Ut , Ekasit Onsaard , Saroat Rawdkuen","doi":"10.1016/j.nfs.2025.100216","DOIUrl":"10.1016/j.nfs.2025.100216","url":null,"abstract":"<div><div>Increasing demand for sustainable and health-conscious food choices has driven a heightened search for alternative protein sources. Plant-based proteins are particularly emphasized for their nutritional benefits, sustainability, and versatility. However, the heavy dependence on soybean has hindered the diversification of plant-based protein sources for global food security amidst population growth. This review underscores the potential of plant protein sources for their novelty as functional food ingredients due to protein quality. These alternatives are also rich in diverse bioactive compounds that offer significant health benefits, positioning them for future food and nutraceutical applications. This review paper explores various potential sources such as rice bran, mung bean, jack bean, moringa seed, and bambara nut, evaluating their suitability based on compositions and amino acid profiles, for use in food formulations. It addresses the robust growth of the plant-based food market and the challenges hindering the full utilization of plant proteins as viable alternatives in shaping the future foods. By addressing gaps in knowledge about emerging plant protein sources, this review aims to enhance sustainability in the food system. Further research and development of alternative plant-based proteins can diversify food offerings while promoting environmental and human health considerations.</div></div>","PeriodicalId":19294,"journal":{"name":"NFS Journal","volume":"38 ","pages":"Article 100216"},"PeriodicalIF":4.1,"publicationDate":"2025-01-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143173332","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
NFS JournalPub Date : 2025-01-23DOI: 10.1016/j.nfs.2025.100217
Ewa Korzeniewska , Ilona Gałązka-Czarnecka , Joanna Sekulska-Nalewajko , Jarosław Gocławski , Tomasz Dróźdż , Paweł Kiełbasa
{"title":"Assessment of changes in vitamin content and morphological characteristics in strawberries modified with a pulsed electric field using chromatography and optical coherence tomography","authors":"Ewa Korzeniewska , Ilona Gałązka-Czarnecka , Joanna Sekulska-Nalewajko , Jarosław Gocławski , Tomasz Dróźdż , Paweł Kiełbasa","doi":"10.1016/j.nfs.2025.100217","DOIUrl":"10.1016/j.nfs.2025.100217","url":null,"abstract":"<div><div>Vitamins B and C are crucial for maintaining human health, so it is important to develop effective methods to support their extraction from products of plant origin. The research conducted by the authors focused on modifying the content of these vitamins in dessert strawberries using a pulsed electric field (PEF) with an intensity of 1, 3 or 5 kV/cm. Experiments have shown that PEF treatment with an intensity of 1 kV/cm and 10 pulses for 10 s resulted in a significant improvement in vitamin content and extraction efficiency of vitamin C by 22 %, folic acid by 24 %, thiamine by more than 50 % and riboflavin by more than 60 %.</div><div>Optical coherence tomography imaging revealed texture changes in fruit pulp under the influence of PEF, related to the release of additional vitamins detected by chromatography methods.</div><div>Additionally, PEF treatment offers significant benefits in fruit processing, including improved dehydration characteristics, reduced time and energy required for freeze-drying, increased extraction efficiency, and potential improvements in bioactive compound content and antioxidant properties.</div><div>The research results may have practical applications in the analytical determination of vitamins C and B by improving the extraction efficiency. Moreover, the use of the PEF method can be important for producers of fruit products (including strawberries), as it enables them to improve the nutritional value of their products.</div></div>","PeriodicalId":19294,"journal":{"name":"NFS Journal","volume":"38 ","pages":"Article 100217"},"PeriodicalIF":4.1,"publicationDate":"2025-01-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143172498","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}