{"title":"Phenolic composition, volatile profile, and in vitro antioxidative potential of hydroethanolic flower extracts of Sambucus nigra L. as a valuable food ingredient","authors":"Jaroslawa Rutkowska , Monika Godlewska , Agata Antoniewska-Krzeska , Agnieszka Nawirska-Olszańska","doi":"10.1016/j.nfs.2025.100248","DOIUrl":"10.1016/j.nfs.2025.100248","url":null,"abstract":"<div><div>Elderberry (<em>Sambucus nigra</em> L.) flowers contain a variety of nutrients and bioactive compounds, and are appreciated in traditional medicine for the treatment of infections. This study aimed to investigate the proper ethanol concentration (20–80 %) in hydroethanolic solvent to obtain <em>Sambucus nigra</em> L. flower extracts rich in bioactive compounds (phenolic and volatiles) and their corresponding antioxidative potential. Extraction yield ranged from 4.8 % to 24.5 % in water and 70 % ethanol extracts, respectively. A higher number of phenolic compounds (41) were identified in hydroethanolic extracts than in water one (31). Hydroethanolic extracts distinguished from water extract with a variety and content of flavonoids (15–19, and 9 compounds, respectively) and a lower number of phenolic acids (10–15 and 20, respectively). The contents of kaempferol dihexoside 1, rutin, and caffeoylquinic acid dominated in the phenolic profile of hydroethanolic extracts. The concentration of ethanol, 20 % and 40 % resulted in efficient extraction of volatiles, 28 and 20 compounds. The l-isoleucine, methyl ester, β-ocimene, citronellol, and citronellyl acetate were assayed as the most important aroma contributors identified in all variant extracts. The enhancement of ethanol in solvent (from 20 % to 80 %) resulted in a 2.5-fold increase in the scavenging activity of DPPH<sup>•</sup>, a 2-fold increase in the scavenging ABTS<sup>•+</sup> radicals, and about a 2-fold increase in reducing power activity measured by FRAP. Results of the study indicate that using a hydroethanolic solvent with 80 % ethanol concentration is the most suitable to obtain <em>Sambucus nigra</em> L. flowers extract rich in phenolic compounds and high antioxidative potential.</div></div>","PeriodicalId":19294,"journal":{"name":"NFS Journal","volume":"41 ","pages":"Article 100248"},"PeriodicalIF":3.3,"publicationDate":"2025-09-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145236306","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
NFS JournalPub Date : 2025-08-21DOI: 10.1016/j.nfs.2025.100242
Ulfah Dwi Kurniawati , Chi Heung Cho , Young Sung Jung , Mingyeong Kim , Ho-Young Park , Sang-Hoon Lee
{"title":"Protective effects of immature Citrus nippokoreana fruit extract on TNF-α/IFN-γ-induced intestinal epithelial barrier disruption","authors":"Ulfah Dwi Kurniawati , Chi Heung Cho , Young Sung Jung , Mingyeong Kim , Ho-Young Park , Sang-Hoon Lee","doi":"10.1016/j.nfs.2025.100242","DOIUrl":"10.1016/j.nfs.2025.100242","url":null,"abstract":"<div><div>This study investigated the protective effects of immature <em>Citrus nippokoreana</em> fruit extract (CFE) against tumor necrosis factor (TNF)-α/interferon (IFN)-γ-induced barrier disruption in human intestinal epithelial cells. CFE demonstrated anti-inflammatory effects by suppressing the increased production of the proinflammatory cytokines interleukin (IL)-1β, IL-6, IL-8, and monocyte chemoattractant protein (MCP)-1. Moreover, CFE maintained barrier integrity, which was evidenced by its ability to prevent reductions in trans-epithelial electrical resistance (TEER) and increments in fluorescein isothiocyanate (FITC)-dextran permeability. The protective activity of CFE was supported by the preservation of the expression and localization of the tight junction proteins zonula occludens (ZO)-1, occludin, and claudin-1. CFE prevented nuclear factor (NF)-κB activation and translocation, myosin light chain kinase (MLCK) upregulation, and myosin light chain II (MLC-2) phosphorylation. The presence of flavonoids, namely didymin, sinensetin, eriocitrin, naringin, narirutin, and hesperidin, was confirmed in CFE. These findings indicate that CFE can serve as a protective agent for maintaining intestinal barrier integrity by suppressing inflammation and preserving tight junctions.</div></div>","PeriodicalId":19294,"journal":{"name":"NFS Journal","volume":"40 ","pages":"Article 100242"},"PeriodicalIF":3.3,"publicationDate":"2025-08-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144893312","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
NFS JournalPub Date : 2025-07-19DOI: 10.1016/j.nfs.2025.100241
Ahmadullah Zahir , Shazia Akhter
{"title":"Exploring the pros and cons of plant-based egg alternatives – A review","authors":"Ahmadullah Zahir , Shazia Akhter","doi":"10.1016/j.nfs.2025.100241","DOIUrl":"10.1016/j.nfs.2025.100241","url":null,"abstract":"<div><div>The global population is projected to surpass 10 billion by 2050, intensifying the demand for agricultural food production to nourish this expanding number. Amidst rising climate change, pollution, and global crises, the increasing need for plant-based food items, including plant-based eggs, presents a viable path for environmental protection and health enhancement, fostering the development and adoption of plant-based egg alternatives. The food industry's growing interest in these egg substitutes is influenced by several factors, including consumer preference, reduced allergen content, improved food safety, healthier nutritional profiles, greater ease of handling and storage, enhanced functionality, cost-effectiveness with reduced price volatility, and a focus on environmental sustainability. These alternatives are commonly derived from various plant-based ingredients, including mung beans, chickpeas, soy, and various starches. Despite the market's expansion, significant challenges for researchers include ensuring the versatility and superior rheological and nutritional properties of these substitutes. Additionally, it is crucial to consider antinutritional factors and allergens present in the plant compounds used for egg alternatives when developing these products. This review will assess the advantages and disadvantages of plant-based egg substitutes, examining key aspects, including nutritional value, environmental impact, taste and texture, cost, and their effect on consumer acceptance and overall health.</div></div>","PeriodicalId":19294,"journal":{"name":"NFS Journal","volume":"40 ","pages":"Article 100241"},"PeriodicalIF":4.1,"publicationDate":"2025-07-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144680382","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
NFS JournalPub Date : 2025-07-11DOI: 10.1016/j.nfs.2025.100240
Benno F. Zimmermann , Eva Maria Daniels , Marieke Katharina Leurs , Linus Rode , Ute Schweiggert-Weisz
{"title":"Comparing methods for the analysis of peptides from in vitro digestion for evaluating the digestibility of plant proteins","authors":"Benno F. Zimmermann , Eva Maria Daniels , Marieke Katharina Leurs , Linus Rode , Ute Schweiggert-Weisz","doi":"10.1016/j.nfs.2025.100240","DOIUrl":"10.1016/j.nfs.2025.100240","url":null,"abstract":"<div><div>In this article, two analytical topics and one nutritional topic are covered. 1. Analytical: the effects of formic acid and trifluoroacetic acid (TFA) at different concentrations in size exclusion chromatography (SEC) for peptide analysis were evaluated. 2. Analytical: three methods for the analysis of peptides after <em>in vitro</em> digestion according to the INFOGEST protocol were compared: photometric quantification after OPA derivatization, SDS–PAGE and SEC. 3. Nutritional: using the most suitable and optimized analytical method, the digestibility of nine protein concentrates from plants and two animal proteins was evaluated.</div><div>The results are as follows: 1. Alternative additives or low TFA concentrations compromise peak resolution. 2. When the digestibility of proteins is evaluated, it is necessary to determine the small peptides that are bioaccessible. This was possible only by SEC. 3. The digestibility of plant proteins – as determined by SEC after <em>in vitro</em> digestion – was found to be in the same range or higher than that of animal proteins.</div></div>","PeriodicalId":19294,"journal":{"name":"NFS Journal","volume":"40 ","pages":"Article 100240"},"PeriodicalIF":4.1,"publicationDate":"2025-07-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144662048","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"A comparative review on novel-assisted extraction techniques for retrieving protein from some potential plant sources","authors":"Paul Ndubuisi Anyiam , Suphat Phongthai , Passakorn Kingwascharapong , Jaksuma Pongsetkul , Wanli Zhang , Young Hoon Jung , Saroat Rawdkuen","doi":"10.1016/j.nfs.2025.100239","DOIUrl":"10.1016/j.nfs.2025.100239","url":null,"abstract":"<div><div>Global protein demand is rising as populations grow and consumers seek sustainable and nutritious alternatives. Plant-based proteins are favored for their consumer acceptance, lower environmental impact, and health benefits. However, the main challenge in extracting high-quality and functional plant proteins for food applications is to select the most suitable extraction method. Conventional protein extraction methods (acid-alkaline and salt extraction) have several drawbacks. Novel-assisted extraction techniques such as enzyme-assisted, ultrasound-assisted, microwave-assisted, high-pressure-assisted, and pulse electric field-assisted extractions, have proven effective in enhancing the yield of high-quality protein. This review examines recent data on novel protein extraction technologies from six promising plant protein sources under optimized conditions, focusing on their yield, protein quality, and functional properties, as well as mechanisms, drawbacks and future perspectives compared to conventional techniques. The final section explores recent trends in enriching foods with protein isolated from some potential plant sources, highlighting their ability to improve food quality and tackle protein deficiencies. The available data indicate that under optimized conditions, cell-disruptive techniques have the potential to improve protein recovery, and functional properties from plant sources, while minimizing environmental pollution. Given the recent surge in interest in alternative plant-based proteins, this review will offer insights and references for future research and development in plant-based protein processing.</div></div>","PeriodicalId":19294,"journal":{"name":"NFS Journal","volume":"40 ","pages":"Article 100239"},"PeriodicalIF":4.1,"publicationDate":"2025-06-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144518779","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
NFS JournalPub Date : 2025-06-13DOI: 10.1016/j.nfs.2025.100235
Santhoshani Warakaulle , Delphine Vincent , Basim Abu-Jdayil , Mutamed M. Ayyash , Afaf Kamal-Eldin
{"title":"Effects of plasmin on camel and bovine model cheeses: Protein degradation, texture, and rheology","authors":"Santhoshani Warakaulle , Delphine Vincent , Basim Abu-Jdayil , Mutamed M. Ayyash , Afaf Kamal-Eldin","doi":"10.1016/j.nfs.2025.100235","DOIUrl":"10.1016/j.nfs.2025.100235","url":null,"abstract":"<div><div>This study investigated the effects of plasmin addition on the physicochemical, textural, and rheological properties throughout the ripening process of cheeses made of camel and bovine milk. Model cheeses were produced with varying plasmin concentrations (0, 0.15, 0.3, and 0.6 U/100 mL milk) to assess changes in physicochemical attributes, casein hydrolysis, hardness, and rheological behavior. Three-way ANOVA demonstrated that the type of milk, plasmin level, ripening duration, and their interactions significantly influenced titratable acidity (%), pH, concentrations of free amino groups, and hardness (<em>p</em> < 0.05). Urea-PAGE analysis revealed changes in CM and BM cheeses during storage, most notably a reduction in the intensity of the β-casein band indicating progressive proteolysis. Ripening resulted in elevated concentrations of free amino groups in both camel and bovine milk cheeses (<em>p</em> < 0.05). The proteolysis of casein during ripening, subsequently leads to increased water retention, which contributes to softer cheese and reduction in both G´ and G´´ values over time.</div></div>","PeriodicalId":19294,"journal":{"name":"NFS Journal","volume":"40 ","pages":"Article 100235"},"PeriodicalIF":4.1,"publicationDate":"2025-06-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144307919","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Oxidative stability and physical properties of model cream-type O/W emulsions structured with candelilla wax and stored at room temperature","authors":"Iwona Szymańska , Anna Żbikowska , Sylwia Onacik-Gür , Małgorzata Kowalska","doi":"10.1016/j.nfs.2025.100233","DOIUrl":"10.1016/j.nfs.2025.100233","url":null,"abstract":"<div><div>Replacing animal-origin products with plant-based raw materials is an environmentally friendly approach and a recommended dietary shift due to its better lipid profile. This study aimed to evaluate the effects of storage time (28 days, 20 °C) on the oxidative stability and physical properties of model cream-type O/W (30/70 m/m) emulsions with candelilla wax-based oleogels (from rapeseed and linseed oils). These emulsions were compared with non-structured emulsions (0 % wax) and a palm oil-based emulsion. The emulsion's primary, secondary, and total degrees of lipid oxidative changes and the microstructure, rheological properties, and physical stability were examined. The oleogelation of the lipid phase in vegan emulsions increased oxidative changes during storage, particularly compared to the palm oil-based emulsion. However, the maximum permissible oxidation levels for refined vegetable oils were not exceeded. The emulsion containing 5 % wax showed the highest oxidative stability among the structured emulsions. Additionally, the changes in the physical parameters of all emulsions were reversible. Emulsions containing 3–5 % wax concentration demonstrated the greatest physical stability among all formulations analyzed. Conversely, 6 or 7 % wax significantly accelerated the emulsions' physical and oxidative destabilization. To preserve the high nutritional value of structured model cream-type emulsions, refrigerated storage and limiting candelilla wax usage to a maximum of 5 % as a stabilizer are suggested. Further research will focus on producing a food product and analyzing its quality and safety, explicitly considering the levels of volatile oxidation products.</div></div>","PeriodicalId":19294,"journal":{"name":"NFS Journal","volume":"39 ","pages":"Article 100233"},"PeriodicalIF":4.1,"publicationDate":"2025-06-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144241009","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
NFS JournalPub Date : 2025-05-23DOI: 10.1016/j.nfs.2025.100231
Anna Kasprzyk
{"title":"Comparison of the amino acid composition and biological value of protein in fallow deer meat from two farming systems","authors":"Anna Kasprzyk","doi":"10.1016/j.nfs.2025.100231","DOIUrl":"10.1016/j.nfs.2025.100231","url":null,"abstract":"<div><div>The primary aim was to determine the protein content, amino acid profile and the biological value of protein in the longissimus lumborum and the semimembranosus muscles of fallow deer originating from an organic system and a conventional system. This is the first study to analyse the amino acid profile and nutritional value of proteins in fallow deer meat from organic farming system. The muscles were collected from 24 fallow deer carcasses with equal proportions of sex and age. The results demonstrated that the follow deer from the organic feeding grounds exhibited higher exogenous and endogenous amino acid contents and nutritional value. A significant effect of the muscle type on the histidine content and the feeding ground × muscle interaction for glycine was noted. In the proteins from fallow deer muscle tissue the content of exogenous amino acids was higher than in the FAO/WHO protein standard. As data regarding fallow deer meat is limited, the results of this study constitute a valuable contribution to knowledge of the meat protein quality. The data presented in this study can be used by dieticians and by breeders. In light of the high quality and nutritional value of this raw material analysed in this paper, it would be advisable to increase its use in the human diet. This meat can be of particular importance in the diets of children and adolescents as well as the elderly and athletes whose protein requirements are elevated.</div></div>","PeriodicalId":19294,"journal":{"name":"NFS Journal","volume":"39 ","pages":"Article 100231"},"PeriodicalIF":4.1,"publicationDate":"2025-05-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144178769","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}