从Hirami柠檬(Citrus reticulata vara . depressa)农业废弃物中提取的精油促进C2C12骨骼肌细胞线粒体生物发生

IF 4.1 Q2 FOOD SCIENCE & TECHNOLOGY
Chung-Hsuan Wang , Ying-Hao Yeo , Chia-Hsin Lin , Mo-rong Xu , Sheng-Yang Wang
{"title":"从Hirami柠檬(Citrus reticulata vara . depressa)农业废弃物中提取的精油促进C2C12骨骼肌细胞线粒体生物发生","authors":"Chung-Hsuan Wang ,&nbsp;Ying-Hao Yeo ,&nbsp;Chia-Hsin Lin ,&nbsp;Mo-rong Xu ,&nbsp;Sheng-Yang Wang","doi":"10.1016/j.nfs.2025.100232","DOIUrl":null,"url":null,"abstract":"<div><div>With increasing awareness of the circular economy, extracting valuable components from agricultural waste offers opportunities to create high-value products. <em>Citrus reticulata</em> var. depressa (CD), endemic to Taiwan, produces sour fruits mainly for juice, leaving significant peel waste. In this study, we extract essential oil (CDEO) from CD peels and examines its effects on skeletal muscle energy metabolism. ATP measurements and mitochondrial staining show that CDEO enhances ATP content, mitochondrial membrane potential, and mass in C<sub>2</sub>C<sub>12</sub> cells. RT-PCR and Western blot analyses indicate that CDEO activates the p-AMPK/SIRT1/PGC-1α pathway and upregulates Nrf1, Nrf2, and TFAM expression. Among the identified constituents, <em>γ</em>-terpinene was found to be the major bioactive compound, capable of promoting ATP production and mitochondrial biogenesis. Future studies should further investigate mitochondrial function and evaluate its anti-fatigue potential using in vivo models.</div></div>","PeriodicalId":19294,"journal":{"name":"NFS Journal","volume":"39 ","pages":"Article 100232"},"PeriodicalIF":4.1000,"publicationDate":"2025-05-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Essential oil extracted from Hirami lemon (Citrus reticulata var. depressa) agricultural waste promotes mitochondrial biogenesis in C2C12 skeletal muscle cells\",\"authors\":\"Chung-Hsuan Wang ,&nbsp;Ying-Hao Yeo ,&nbsp;Chia-Hsin Lin ,&nbsp;Mo-rong Xu ,&nbsp;Sheng-Yang Wang\",\"doi\":\"10.1016/j.nfs.2025.100232\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><div>With increasing awareness of the circular economy, extracting valuable components from agricultural waste offers opportunities to create high-value products. <em>Citrus reticulata</em> var. depressa (CD), endemic to Taiwan, produces sour fruits mainly for juice, leaving significant peel waste. In this study, we extract essential oil (CDEO) from CD peels and examines its effects on skeletal muscle energy metabolism. ATP measurements and mitochondrial staining show that CDEO enhances ATP content, mitochondrial membrane potential, and mass in C<sub>2</sub>C<sub>12</sub> cells. RT-PCR and Western blot analyses indicate that CDEO activates the p-AMPK/SIRT1/PGC-1α pathway and upregulates Nrf1, Nrf2, and TFAM expression. Among the identified constituents, <em>γ</em>-terpinene was found to be the major bioactive compound, capable of promoting ATP production and mitochondrial biogenesis. Future studies should further investigate mitochondrial function and evaluate its anti-fatigue potential using in vivo models.</div></div>\",\"PeriodicalId\":19294,\"journal\":{\"name\":\"NFS Journal\",\"volume\":\"39 \",\"pages\":\"Article 100232\"},\"PeriodicalIF\":4.1000,\"publicationDate\":\"2025-05-23\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"NFS Journal\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S2352364625000215\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q2\",\"JCRName\":\"FOOD SCIENCE & TECHNOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"NFS Journal","FirstCategoryId":"1085","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S2352364625000215","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"FOOD SCIENCE & TECHNOLOGY","Score":null,"Total":0}
引用次数: 0

摘要

随着循环经济意识的增强,从农业废弃物中提取有价值的成分为创造高价值产品提供了机会。台湾特有种柑橘(Citrus reticulata var. depressa, CD)产酸果,主要为榨汁用,果皮浪费严重。在这项研究中,我们从CD皮中提取精油(CDEO),并研究其对骨骼肌能量代谢的影响。ATP测量和线粒体染色显示,CDEO提高了C2C12细胞的ATP含量、线粒体膜电位和质量。RT-PCR和Western blot分析表明,CDEO激活p-AMPK/SIRT1/PGC-1α通路,上调Nrf1、Nrf2和TFAM的表达。在鉴定的成分中,γ-萜烯被发现是主要的生物活性化合物,能够促进ATP的产生和线粒体的生物发生。未来的研究应进一步研究线粒体功能,并利用体内模型评估其抗疲劳潜力。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Essential oil extracted from Hirami lemon (Citrus reticulata var. depressa) agricultural waste promotes mitochondrial biogenesis in C2C12 skeletal muscle cells

Essential oil extracted from Hirami lemon (Citrus reticulata var. depressa) agricultural waste promotes mitochondrial biogenesis in C2C12 skeletal muscle cells
With increasing awareness of the circular economy, extracting valuable components from agricultural waste offers opportunities to create high-value products. Citrus reticulata var. depressa (CD), endemic to Taiwan, produces sour fruits mainly for juice, leaving significant peel waste. In this study, we extract essential oil (CDEO) from CD peels and examines its effects on skeletal muscle energy metabolism. ATP measurements and mitochondrial staining show that CDEO enhances ATP content, mitochondrial membrane potential, and mass in C2C12 cells. RT-PCR and Western blot analyses indicate that CDEO activates the p-AMPK/SIRT1/PGC-1α pathway and upregulates Nrf1, Nrf2, and TFAM expression. Among the identified constituents, γ-terpinene was found to be the major bioactive compound, capable of promoting ATP production and mitochondrial biogenesis. Future studies should further investigate mitochondrial function and evaluate its anti-fatigue potential using in vivo models.
求助全文
通过发布文献求助,成功后即可免费获取论文全文。 去求助
来源期刊
NFS Journal
NFS Journal Agricultural and Biological Sciences-Food Science
CiteScore
11.10
自引率
0.00%
发文量
18
审稿时长
29 days
期刊介绍: The NFS Journal publishes high-quality original research articles and methods papers presenting cutting-edge scientific advances as well as review articles on current topics in all areas of nutrition and food science. The journal particularly invites submission of articles that deal with subjects on the interface of nutrition and food research and thus connect both disciplines. The journal offers a new form of submission Registered Reports (see below). NFS Journal is a forum for research in the following areas: • Understanding the role of dietary factors (macronutrients and micronutrients, phytochemicals, bioactive lipids and peptides etc.) in disease prevention and maintenance of optimum health • Prevention of diet- and age-related pathologies by nutritional approaches • Advances in food technology and food formulation (e.g. novel strategies to reduce salt, sugar, or trans-fat contents etc.) • Nutrition and food genomics, transcriptomics, proteomics, and metabolomics • Identification and characterization of food components • Dietary sources and intake of nutrients and bioactive compounds • Food authentication and quality • Nanotechnology in nutritional and food sciences • (Bio-) Functional properties of foods • Development and validation of novel analytical and research methods • Age- and gender-differences in biological activities and the bioavailability of vitamins, minerals, and phytochemicals and other dietary factors • Food safety and toxicology • Food and nutrition security • Sustainability of food production
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:604180095
Book学术官方微信