Neuropeptides最新文献_第7页

CDNF overexpression prevents motor-cognitive dysfunction by intrastriatal CPP-based delivery system in a Parkinson's disease animal model 在帕金森病动物模型中，CDNF过表达通过基于CPP的三段内递送系统预防运动认知功能障碍。

IF 2.9 3区医学

Neuropeptides Pub Date : 2023-10-11 DOI: 10.1016/j.npep.2023.102385

Sheila A. Villa-Cedillo , Daniel Matta-Yee-Chig , Adolfo Soto-Domínguez , Humberto Rodríguez-Rocha , Aracely García-García , Carlos R. Montes-de-Oca-Saucedo , María de Jesús Loera-Arias , Jesús Valdés , Odila Saucedo-Cárdenas

{"title":"CDNF overexpression prevents motor-cognitive dysfunction by intrastriatal CPP-based delivery system in a Parkinson's disease animal model","authors":"Sheila A. Villa-Cedillo , Daniel Matta-Yee-Chig , Adolfo Soto-Domínguez , Humberto Rodríguez-Rocha , Aracely García-García , Carlos R. Montes-de-Oca-Saucedo , María de Jesús Loera-Arias , Jesús Valdés , Odila Saucedo-Cárdenas","doi":"10.1016/j.npep.2023.102385","DOIUrl":"10.1016/j.npep.2023.102385","url":null,"abstract":"<div><p><span><span><span><span>Parkinson's disease<span><span> (PD) is characterized by the loss of dopaminergic neurons in the </span>substantia nigra pars compact (SNpc), and no effective </span></span>treatment<span> has yet been established to prevent PD. Neurotrophic factors<span>, such as cerebral dopamine neurotrophic factor (CDNF), have shown a neuroprotective effect on dopaminergic neurons. Previously, we developed a cell-penetrating-peptide-based delivery system that includes Asn194Lys mutation in the rabies virus glycoprotein-9R peptide (mRVG9R), which demonstrated a higher delivery rate than the wild-type. In this study, using a mouse PD-like model, we evaluated the intrastriatal mRVG9R-KP-CDNF gene therapy through motor and </span></span></span>cognitive tests<span> and brain cell analysis. The mRVG9R-KP-CDNF complex was injected into the striatum on days 0 and 20. To induce the PD-like model, mice were intraperitoneally administered Paraquat (PQ) twice a week for 6 weeks. Our findings demonstrate that mRVG9R-KP-CDNF gene therapy effectively protects brain cells from PQ toxicity and prevents motor and </span></span>cognitive dysfunction<span> in mice. We propose that the mRVG9R-KP-CDNF complex inhibits astrogliosis<span><span> and microglia activation, safeguarding dopaminergic neurons and </span>oligodendrocytes from PQ-induced damage. This study presents an efficient CDNF delivery system, protecting neurons and glia in the </span></span></span>nigrostriatal pathway<span> from PQ-induced damage, which is known to lead to motor and cognitive dysfunction in neurodegenerative diseases such as PD.</span></p></div>","PeriodicalId":19254,"journal":{"name":"Neuropeptides","volume":"102 ","pages":"Article 102385"},"PeriodicalIF":2.9,"publicationDate":"2023-10-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41207242","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Apelin receptor antagonist (ML221) treatment has a stimulatory effect on the testicular proliferation, antioxidants system and steroidogenesis in adult mice Apelin受体拮抗剂(ML221)对成年小鼠睾丸增生、抗氧化系统和甾体生成具有刺激作用

IF 2.9 3区医学

Neuropeptides Pub Date : 2023-10-01 DOI: 10.1016/j.npep.2023.102354

Milirani Das, Guruswami Gurusubramanian, Vikas Kumar Roy

{"title":"Apelin receptor antagonist (ML221) treatment has a stimulatory effect on the testicular proliferation, antioxidants system and steroidogenesis in adult mice","authors":"Milirani Das, Guruswami Gurusubramanian, Vikas Kumar Roy","doi":"10.1016/j.npep.2023.102354","DOIUrl":"10.1016/j.npep.2023.102354","url":null,"abstract":"<div><p><span><span><span>The expression of apelin<span> and its receptor (APJ) has been shown in the hypothalamus-pituitary-testicular axis. It has also been suggested apelin and APJ are neuropeptide factors. The presence of apelin and APJ in the </span></span>seminiferous tubules and </span>interstitium<span> might be predicted to act as a local regulator of testicular activity, although the function is not well known in the mice testis. In the present study, we have investigated the effects of APJ, antagonist, ML221 on the </span></span>gonadotropin<span><span> levels, testicular steroidogenesis, proliferation, </span>apoptosis<span> and antioxidant system. Our results showed that inhibition of APJ by ML221 increased the sperm concentration, circulating testosterone, FSH, LH levels and intra-testicular testosterone concentration. Furthermore, ML221 treatment<span><span><span> stimulates the germ cell proliferation and antioxidant system in the testis. The expression of BCL2, </span>AR<span><span> was up-regulated whereas, the expression of BAX and active caspase3 was down-regulated after ML221 treatment. Immunohistocehmical analysis of AR also showed increase abundance in the spermatogonia, primary </span>spermatocytes<span> and Leydig cells of 150 μg/kg dose group. These findings suggest that in adult testis, the apelin system might have an inhibitory role in germ cell proliferation and a stimulatory role in apoptosis. It might also be suggested that the apelin system could be involved in the disposal mechanism for damaged germ cells during </span></span></span>spermatogenesis via the down-regulation of AR.</span></span></span></p></div>","PeriodicalId":19254,"journal":{"name":"Neuropeptides","volume":"101 ","pages":"Article 102354"},"PeriodicalIF":2.9,"publicationDate":"2023-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10166597","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Anandamide improves food intake and orexinergic neuronal activity in the chronic sleep deprivation induction model in rats by modulating the expression of the CB1 receptor in the lateral hypothalamus Anandamide通过调节下丘脑外侧CB1受体的表达，改善大鼠慢性睡眠剥夺诱导模型中的食物摄入和食欲能神经元活性

IF 2.9 3区医学

Neuropeptides Pub Date : 2023-10-01 DOI: 10.1016/j.npep.2023.102336

Rafie Belali , Seyyed Ali Mard , Seyed Esmaeil Khoshnam , Kowsar Bavarsad , Alireza Sarkaki , Yaghoob Farbood

{"title":"Anandamide improves food intake and orexinergic neuronal activity in the chronic sleep deprivation induction model in rats by modulating the expression of the CB1 receptor in the lateral hypothalamus","authors":"Rafie Belali , Seyyed Ali Mard , Seyed Esmaeil Khoshnam , Kowsar Bavarsad , Alireza Sarkaki , Yaghoob Farbood","doi":"10.1016/j.npep.2023.102336","DOIUrl":"10.1016/j.npep.2023.102336","url":null,"abstract":"<div><p><span><span><span>Sleep deprivation alters orexinergic neuronal activity in the lateral </span>hypothalamus (LH), which is the main regulator of sleep-wake, arousal, appetite, and energy regulation processes. </span>Cannabinoid receptor<span><span> (CBR) expression in this area is involved in modulating the function of orexin<span> neurons. In this study, we investigated the effects of endocannabinoid </span></span>anandamide<span> (AEA) administration on improving food intake and appetite by modulating the activity of orexin neurons and CB1R expression after chronic sleep deprivation. Adult male Wistar rats<span><span> (200–250 g) were randomly divided into three groups: control + vehicle (Control), chronic sleep deprivation + vehicle (SD), and chronic sleep deprivation +20 mg/kg AEA (SD + A). For SD induction, the rats were kept in a sleep deprivation device for 18 h (7 a.m. to 1 a.m.) daily for 21 days. Weight gain, food intake, the electrical power of orexin neurons, CB1R mRNA expression in hypothalamus, CB1R protein expression in the LH, TNF-α, IL-6, IL-4 levels and </span>antioxidant activity in hypothalamus were measured after SD induction. Our results showed that AEA administration significantly improved food intake (</span></span></span></span><em>p</em> < 0.01), Electrical activity of orexin neurons (<em>p</em> < 0.05), CB1R expression in the hypothalamus (p < 0.05), and IL-4 levels (<em>p</em> < 0.05). AEA also reduced mRNA expression of OX1R and OX2R (<em>p</em><span> < 0.01 and p < 0.05 respectively), also IL-6 and TNF-α (p < 0.01) and MDA<span><span> level (p < 0.05) in hypothalamic tissue. As a consequence, AEA modulates orexinergic system function and improves food intake by regulating the expression of the </span>CB1 receptor in the LH in sleep deprived rats.</span></span></p></div>","PeriodicalId":19254,"journal":{"name":"Neuropeptides","volume":"101 ","pages":"Article 102336"},"PeriodicalIF":2.9,"publicationDate":"2023-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10519350","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Hippocampal pituitary adenylate cyclase–activating polypeptide mediates rapid antidepressant-like effects of Yueju pill 海马垂体腺苷酸环化酶激活多肽介导粤菊丸快速抗抑郁样作用

IF 2.9 3区医学

Neuropeptides Pub Date : 2023-10-01 DOI: 10.1016/j.npep.2023.102350

Li Ren , Hailou Zhang , Weiwei Tao , Wenda Xue , Yin Chen , Zhilu Zou , Xiaoyan Guo , Qinqin Shen , Wei Wang , Haitang Jiang , Juanjuan Tang , Quansheng Feng , Gang Chen

{"title":"Hippocampal pituitary adenylate cyclase–activating polypeptide mediates rapid antidepressant-like effects of Yueju pill","authors":"Li Ren , Hailou Zhang , Weiwei Tao , Wenda Xue , Yin Chen , Zhilu Zou , Xiaoyan Guo , Qinqin Shen , Wei Wang , Haitang Jiang , Juanjuan Tang , Quansheng Feng , Gang Chen","doi":"10.1016/j.npep.2023.102350","DOIUrl":"10.1016/j.npep.2023.102350","url":null,"abstract":"<div><p>Yueju pill, a classic Chinese Medicine formulated, was recently found to produce rapid antidepressant-like effects in a PKA-CREB signaling-dependent manner. In our study, we found that the Yueju pill induced a remarkable increase in PACAP. The intracerebroventricular injection of PACAP agonist induced a rapid antidepressant-like effect; conversely, the intrahippocampal infusion of a PACAP antagonist reversed the antidepressant response of the Yueju pill. Mice with hippocampal PACAP knockdown via viral-mediated RNAi displayed depression-like behavior. PACAP knockdown also blunted the antidepressant effect of the Yueju pill. PACAP knockdown resulted in down-regulated CREB and expression of the synaptic protein PSD95 at both baselines and after administration of the Yueju pill. However, administration of the Yueju pill in the knockdown mice promoted PACAP and PKA levels. Chronically stressed mice showed deficient hippocampal PACAP-PKA-CREB signaling and depression-like behavior, which were reversed by a single dose of the Yueju pill. In this study, we demonstrated that the up-regulation of PACAP induced activating of PKA-CREB signaling would play a part in the rapid antidepressant-like effects of the Yueju pill. We also identified iridoids fraction of <em>Gardenia jasminoides</em> Ellis (GJ-IF), a vital component of the Yueju pill, was identified to recapitulate rapid antidepressant-like behavior through increased hippocampal PACAP expression of the Yueju pill. The promotion of hippocampal PACAP may collectively represent a novel mechanism of rapid antidepressant-like effect.</p></div>","PeriodicalId":19254,"journal":{"name":"Neuropeptides","volume":"101 ","pages":"Article 102350"},"PeriodicalIF":2.9,"publicationDate":"2023-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10156663","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Oxytocin as neuro-hormone and neuro-regulator exert neuroprotective properties: A mechanistic graphical review 催产素作为神经激素和神经调节剂发挥神经保护作用:一个机制的图形回顾

IF 2.9 3区医学

Neuropeptides Pub Date : 2023-10-01 DOI: 10.1016/j.npep.2023.102352

Roya Kamrani-Sharif , A. Wallace Hayes , Mina Gholami , Mahsa Salehirad , Maryam Allahverdikhani , Majid Motaghinejad , Enzo Emanuele

{"title":"Oxytocin as neuro-hormone and neuro-regulator exert neuroprotective properties: A mechanistic graphical review","authors":"Roya Kamrani-Sharif , A. Wallace Hayes , Mina Gholami , Mahsa Salehirad , Maryam Allahverdikhani , Majid Motaghinejad , Enzo Emanuele","doi":"10.1016/j.npep.2023.102352","DOIUrl":"10.1016/j.npep.2023.102352","url":null,"abstract":"<div><h3>Background</h3><p><span>Neurodegeneration is progressive cell loss in specific neuronal populations, often resulting in clinical consequences with significant medical, societal, and economic implications. Because of its antioxidant, anti-inflammatory, and anti-apoptotic properties, oxytocin has been proposed as a potential </span>neuroprotective and neurobehavioral therapeutic agent, including modulating mood disturbances and cognitive enchantment.</p></div><div><h3>Methods</h3><p><span>Literature searches were conducted using the following databases Web of Science, PubMed, Elsevier Science Direct, Google Scholar, the Core Collection, and Cochrane from January 2000 to February 2023 for articles dealing with oxytocin neuroprotective properties in preventing or treating neurodegenerative disorders and diseases with a focus on </span>oxidative stress, inflammation, and apoptosis/cell death.</p></div><div><h3>Results</h3><p><span><span>The neuroprotective effects of oxytocin appears to be mediated by its anti-inflammatory properties, inhibition of neuro inflammation, activation of several antioxidant enzymes, inhibition of oxidative stress and </span>free radical formation, activation of free radical scavengers, prevent of mitochondrial dysfunction, and inhibition of </span>apoptosis.</p></div><div><h3>Conclusion</h3><p>Oxytocin acts as a neuroprotective agent by preventing neuro-apoptosis, neuro-inflammation, and neuronal oxidative stress, and by restoring mitochondrial function.</p></div>","PeriodicalId":19254,"journal":{"name":"Neuropeptides","volume":"101 ","pages":"Article 102352"},"PeriodicalIF":2.9,"publicationDate":"2023-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10163227","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Role and therapeutic target of P2X2/3 receptors in visceral pain P2X2/3受体在内脏疼痛中的作用及治疗靶点

IF 2.9 3区医学

Neuropeptides Pub Date : 2023-10-01 DOI: 10.1016/j.npep.2023.102355

Hong-mei Luo , Jia-rong Ye , Fan-qin Pu , Hong-liang Luo , Wen-jun Zhang

引用次数: 1

Bee venom ameliorates oxidative stress and histopathological changes of hippocampus, liver and testis during status epileptics 蜂毒可改善癫痫状态时海马、肝脏和睾丸的氧化应激和组织病理学改变

IF 2.9 3区医学

Neuropeptides Pub Date : 2023-10-01 DOI: 10.1016/j.npep.2023.102368

Esraa K. Aly , Hanan S. Mahmoud , Dalal Hussien M. Alkhalifah , Gaber M.G. Shehab , Abdelaziz S.A. Abuelsaad , Eman S. Abdel-Rehiem , Manal Abdul-Hamid

{"title":"Bee venom ameliorates oxidative stress and histopathological changes of hippocampus, liver and testis during status epileptics","authors":"Esraa K. Aly , Hanan S. Mahmoud , Dalal Hussien M. Alkhalifah , Gaber M.G. Shehab , Abdelaziz S.A. Abuelsaad , Eman S. Abdel-Rehiem , Manal Abdul-Hamid","doi":"10.1016/j.npep.2023.102368","DOIUrl":"10.1016/j.npep.2023.102368","url":null,"abstract":"<div><p><span><span><span>The unrelenting progression of neurodegenerative diseases<span><span> has a negative impact on affected individuals, their families, and society. Recurrent epileptic seizures are the hallmark of epilepsy, and treating it effectively remains difficult. Clarify and understanding effects of the </span>antiepileptic drugs<span><span><span> (AEDs) in epilepsy by comparing the therapeutic effects between rats receiving valproic acid (VPA) and </span>Bee venom<span> (BV) was aimed throughout the present study. Four male Wistar rat<span> groups were included: control, epileptic group receiving pilocarpine<span> (PILO), epileptic group treated with VPA and BV respectively. Cognitive functions were assessed by evaluating latency time in hot plate, </span></span></span></span>despair<span> swim test, grooming, rearing and ambulation frequency in the open field. BV has ameliorative effect on electrolytes balancing, assured by decreasing lipid peroxidation<span><span>, nitric oxide and increasing </span>catalase, </span></span></span></span></span>superoxide dismutase and </span>glutathione peroxidase<span><span> activities. BV enhanced restoration of liver functions indicated by alanine transaminase (ALT) and </span>aspartate transaminase (AST), total proteins, and albumin; hormonal parameters total and </span></span>free testosterone<span><span>, follicle stimulating hormone<span> (FSH) and Luteinizing hormone<span> (LH) were preserved by BV with great recovery of hippocampus, liver and testicular </span></span></span>histopathology<span> and ultrastructure comparing with the epileptic rats. The present findings suggested that BV and its active components offer fresh options for controlling epilepsy and prospective methods via minimize or manage the severe consequences.</span></span></p></div>","PeriodicalId":19254,"journal":{"name":"Neuropeptides","volume":"101 ","pages":"Article 102368"},"PeriodicalIF":2.9,"publicationDate":"2023-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10520891","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

FGF-18 alleviates memory impairments and neuropathological changes in a rat model of Alzheimer's disease FGF-18减轻阿尔茨海默病大鼠模型的记忆障碍和神经病理改变

IF 2.9 3区医学

Neuropeptides Pub Date : 2023-10-01 DOI: 10.1016/j.npep.2023.102367

Arzuhan Cetindag Ciltas , Sebahattin Karabulut , Bilal Sahin , Ahmet Kemal Filiz , Fatih Yulak , Mustafa Ozkaraca , Ozhan Karatas , Ali Cetin

{"title":"FGF-18 alleviates memory impairments and neuropathological changes in a rat model of Alzheimer's disease","authors":"Arzuhan Cetindag Ciltas , Sebahattin Karabulut , Bilal Sahin , Ahmet Kemal Filiz , Fatih Yulak , Mustafa Ozkaraca , Ozhan Karatas , Ali Cetin","doi":"10.1016/j.npep.2023.102367","DOIUrl":"10.1016/j.npep.2023.102367","url":null,"abstract":"<div><p><span><span>Alzheimer's disease (AD) is a multifactorial pathology marked by </span>amyloid beta<span><span> (Aβ) accumulation, tau hyperphosphorylation, and progressive cognitive decline. Previous studies show that </span>fibroblast growth factor 18<span> (FGF18) exerts a neuroprotective<span> effect in experimental models of neurodegeneration; however, how it affects AD pathology remains unknown. This study aimed to ascertain the impact of FGF18 on the behavioral and neuropathological changes in the rat model of sporadic AD induced by intracerebroventricular (ICV) injection of </span></span></span></span>streptozotocin<span><span> (STZ). The rats were treated with FGF18 (0.94 and 1.88 pmol, ICV) on the 15th day after STZ injection. Their cognitive function was assessed in the Morris water maze and </span>passive avoidance<span><span><span> tests for 5 days from the 16th to the 21st days. Aβ levels and histological signs of neurotoxicity were detected using the enzyme-linked immunosorbent assay (ELISA) assay and histopathological analysis of the brain, respectively. FGF18 mildly ameliorated the STZ-induced </span>cognitive impairment; the Aβ accumulation was reduced; and the neuronal damage including </span>pyknosis<span> and apoptosis was alleviated in the rat brain. This study highlights the promising therapeutic potential for FGF18 in managing AD.</span></span></span></p></div>","PeriodicalId":19254,"journal":{"name":"Neuropeptides","volume":"101 ","pages":"Article 102367"},"PeriodicalIF":2.9,"publicationDate":"2023-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10164331","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Protective effect of TNIP2 on the inflammatory response of microglia after spinal cord injury in rats TNIP2对脊髓损伤后小胶质细胞炎症反应的保护作用

IF 2.9 3区医学

Neuropeptides Pub Date : 2023-10-01 DOI: 10.1016/j.npep.2023.102351

Jiawei Fu , Chunshuai Wu , Guanhua Xu , Jinlong Zhang , Jiajia Chen , Chu Chen , Hongxiang Hong , Pengfei Xue , Jiawei Jiang , Jiayi Huang , Chunyan Ji , Zhiming Cui

{"title":"Protective effect of TNIP2 on the inflammatory response of microglia after spinal cord injury in rats","authors":"Jiawei Fu , Chunshuai Wu , Guanhua Xu , Jinlong Zhang , Jiajia Chen , Chu Chen , Hongxiang Hong , Pengfei Xue , Jiawei Jiang , Jiayi Huang , Chunyan Ji , Zhiming Cui","doi":"10.1016/j.npep.2023.102351","DOIUrl":"10.1016/j.npep.2023.102351","url":null,"abstract":"<div><h3>Background</h3><p>Spinal cord injury<span> (SCI) is a devastating disease that can lead to tissue loss and neurological dysfunction. TNIP2 is a negative regulator of NF-κB signaling due to its capacity to bind A20 and suppress inflammatory cytokines-induced NF-κB activation. However, the anti-inflammatory role of TNIP2 in SCI remains unclear. Our study's intention was to evaluate the effect of TNIP2 on the inflammatory response of microglia after spinal cord injury in rats.</span></p></div><div><h3>Methods</h3><p><span><span>HE </span>staining<span> and Nissl staining were performed on day 3 following SCI to analyze the histological changes. To further investigate the functional changes of TNIP2 after SCI, we performed immunofluorescence staining experiments. The effect of </span></span>LPS<span> on TNIP2 expression in BV2 cells was examined by western blot. The levels of TNF-α, IL-1β, and IL-6 in spinal cord tissues of rats with SCI and in BV2 cells with LPS were measured by using qPCR.</span></p></div><div><h3>Results</h3><p><span>TNIP2 expression was closely associated with the pathophysiology of SCI in rats, and TNIP2 was involved in regulating functional changes in microglia. TNIP2 expression was increased during SCI in rats and that overexpression of TNIP2 inhibited M1 polarization and pro-inflammatory cytokine production in microglia, which might ultimately protect against inflammatory responses through the </span>MAPK<span> and NF-κB signaling pathways.</span></p></div><div><h3>Conclusions</h3><p>The present study provides evidence for a role of TNIP2 in the regulation of inflammation in SCI and suggests that induction of TNIP2 expression alleviated the inflammatory response of microglia.</p></div>","PeriodicalId":19254,"journal":{"name":"Neuropeptides","volume":"101 ","pages":"Article 102351"},"PeriodicalIF":2.9,"publicationDate":"2023-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10249146","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Restraint stress potentiates sensitivity to the antinociceptive effect of morphine through orexin receptors in the ventral tegmental area 约束应激通过腹侧被盖区的食欲素受体增强了对吗啡抗感觉作用的敏感性

IF 2.9 3区医学

Neuropeptides Pub Date : 2023-10-01 DOI: 10.1016/j.npep.2023.102353

Sajad Mazaheri , Morteza Zendehdel , Abbas Haghparast

{"title":"Restraint stress potentiates sensitivity to the antinociceptive effect of morphine through orexin receptors in the ventral tegmental area","authors":"Sajad Mazaheri , Morteza Zendehdel , Abbas Haghparast","doi":"10.1016/j.npep.2023.102353","DOIUrl":"10.1016/j.npep.2023.102353","url":null,"abstract":"<div><p><span><span>Orexin<span> signaling in the ventral tegmental area (VTA) plays a critical role in stress and </span></span>addictive behaviors<span>. On the other hand, exposure to stress potentiates behavioral sensitization to drugs<span> of abuse such as morphine. This study aimed to elucidate the role of orexin receptors within the VTA in restraint stress (</span></span></span><em>RS</em><span><span>)-induced morphine sensitization. Adult male albino Wistar rats underwent </span>stereotaxic surgery<span>, and two stainless steel guide cannulae<span> were bilaterally implanted into the VTA. Different doses of SB334867<span> or TCS OX2 29 as orexin-1 (OX1) and orexin-2 (OX2) receptor antagonists were microinjected into the VTA five min before exposure to RS, respectively. A duration of three hours was considered for applying the RS, and 10 min after RS exposure, animals received a subcutaneous injection of an ineffective dose of morphine (1 mg/kg) for three consecutive days followed by a five-day drug/stress-free period. On the ninth day, the tail-flick test evaluated the sensitivity to the antinociceptive effects of morphine. The results demonstrated that the sole application of RS or morphine (1 mg/kg) could not induce morphine sensitization; however, concurrent application of RS and morphine could induce morphine sensitization. Besides, intra-VTA administration of OX1 R or OX2 R antagonists before paired administration of morphine and RS blocked morphine sensitization. The role of OX1 R and OX2 R in the induction of stress-induced morphine sensitization was almost identical. This study provides new insight into the role of orexin signaling in the VTA in the potentiation of morphine sensitization induced by RS and morphine co-administration.</span></span></span></span></p></div>","PeriodicalId":19254,"journal":{"name":"Neuropeptides","volume":"101 ","pages":"Article 102353"},"PeriodicalIF":2.9,"publicationDate":"2023-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10165769","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0