Neuroreport最新文献_第2页

Predicting the effectiveness of binaural beats on working memory. 预测双耳节拍对工作记忆的影响

IF 1.6 4区医学

Neuroreport Pub Date : 2024-12-04 Epub Date: 2024-09-30 DOI: 10.1097/WNR.0000000000002101

Ahmad Zahid Rao, Muhammad Danish Mujib, Saad Ahmed Qazi, Ahmad O Alokaily, Ayesha Ikhlaq, Eraj Humayun Mirza, Ahmed Ali Aldohbeyb, Muhammad Abul Hasan

{"title":"Predicting the effectiveness of binaural beats on working memory.","authors":"Ahmad Zahid Rao, Muhammad Danish Mujib, Saad Ahmed Qazi, Ahmad O Alokaily, Ayesha Ikhlaq, Eraj Humayun Mirza, Ahmed Ali Aldohbeyb, Muhammad Abul Hasan","doi":"10.1097/WNR.0000000000002101","DOIUrl":"10.1097/WNR.0000000000002101","url":null,"abstract":"Working memory is vital for short-term information processing. Binaural beats can enhance working memory by improving attention and memory consolidation through neural synchronization. However, individual differences in cognitive and neuronal functioning affect effectiveness of binaural beats, necessitating personalized approaches. This study aimed to develop a machine learning model to predict binaural beats's effectiveness on working memory using electroencephalography. Sixty healthy participants underwent a 5-min electroencephalography recording, an initial working memory evaluation, 15 min of binaural beats stimulation, and a subsequent working memory evaluation using digit span tests of increasing difficulty. Recall accuracy and response times were measured. Differential scores from pre-evaluation and post-evaluation labeled participants as active or inactive to binaural beats stimulation. electroencephalography data, recorded using 14 electrodes, provided brain activity estimates across theta, alpha, beta, and gamma frequency bands, resulting in 56 features (14 channels × 4 bands) for the machine learning model. Several classifiers were tested to identify the most effective model. The weighted K-nearest neighbors model achieved the highest accuracy (90.0%) and area under the receiver operating characteristic curve (92.24%). Frontal and parietal electroencephalography channels in theta and alpha bands were crucial for classification. This study's findings offer significant clinical insights, enabling informed interventions and preventing resource inefficiency.","PeriodicalId":19213,"journal":{"name":"Neuroreport","volume":" ","pages":"1082-1089"},"PeriodicalIF":1.6,"publicationDate":"2024-12-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142470984","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Gray matter volume differences based on sex in first-episode drug-naive patients with major depressive disorder and its molecular analysis. 基于性别的重度抑郁障碍首发药物依赖者灰质体积差异及其分子分析。

IF 1.6 4区医学

Neuroreport Pub Date : 2024-12-04 Epub Date: 2024-10-10 DOI: 10.1097/WNR.0000000000002107

Duo Sun, Kang Xi, Runxu Yang, Jiangmin Chu, Mingjie Xu, Dafu Zhang, Yuqi Cheng

{"title":"Gray matter volume differences based on sex in first-episode drug-naive patients with major depressive disorder and its molecular analysis.","authors":"Duo Sun, Kang Xi, Runxu Yang, Jiangmin Chu, Mingjie Xu, Dafu Zhang, Yuqi Cheng","doi":"10.1097/WNR.0000000000002107","DOIUrl":"10.1097/WNR.0000000000002107","url":null,"abstract":"This study analyzed whether gray matter volume (GMV) differences exist between the sexes in patients with major depressive disorder (MDD) and explored the relationships between these differences and neurotransmitter systems. This study enrolled 190 first-episode drug-naive patients with MDD and 293 healthy controls. All participants underwent T1-weighted high-resolution MRI. The interaction between the diagnosis (healthy controls vs. MDD) and sex (male vs. female) regarding GMV alterations was analyzed. The JuSpace toolbox, which covers a wide range of neurotransmitter systems, was used to identify the relationship between MDD-induced and sex-induced GMV alterations and specific receptor/transporter proteins in the brain. Sex-specific GMV differences were observed in the healthy controls but not in MDD patients. Male healthy controls had a larger GMV in the bilateral parahippocampal, lingual, inferior occipital, fusiform, cerebellar subregions, and left inferior temporal than female healthy controls, but several subregions of the thalamus had a larger GMV in female healthy controls than in male healthy controls. Sex-induced GMV alterations were associated with 5-hydroxytryptamine receptor subtype 1a, cannabinoid receptor, and dopamine receptor ( P < 0.01, false discovery rate corrected). GMV differences were not detected in the main effect of diagnosis and the interaction of diagnosis and sex. Sex-specific GMV differences are associated with the spatial distribution of serotonin, dopamine, and cannabinoid neurotransmitter receptor systems. Sex-based physiological differences in the GMV may account for male and female susceptibility to and differences in the clinical symptoms of MDD.","PeriodicalId":19213,"journal":{"name":"Neuroreport","volume":" ","pages":"1117-1122"},"PeriodicalIF":1.6,"publicationDate":"2024-12-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142471001","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Aberrant dynamic functional and effective connectivity changes of the primary visual cortex in patients with retinal detachment via machine learning. 通过机器学习研究视网膜脱离患者初级视觉皮层的动态功能和有效连接性异常变化。

IF 1.6 4区医学

Neuroreport Pub Date : 2024-12-04 Epub Date: 2024-10-03 DOI: 10.1097/WNR.0000000000002100

Yu Ji, Yuan-Yuan Wang, Qi Cheng, Wen-Wen Fu, Ben-Liang Shu, Bin Wei, Qin-Yi Huang, Xiao-Rong Wu

{"title":"Aberrant dynamic functional and effective connectivity changes of the primary visual cortex in patients with retinal detachment via machine learning.","authors":"Yu Ji, Yuan-Yuan Wang, Qi Cheng, Wen-Wen Fu, Ben-Liang Shu, Bin Wei, Qin-Yi Huang, Xiao-Rong Wu","doi":"10.1097/WNR.0000000000002100","DOIUrl":"10.1097/WNR.0000000000002100","url":null,"abstract":"Objective: Previous neuroimaging studies have identified significant alterations in brain functional activity in retinal detachment (RD) patients, these investigations predominantly concentrated on local functional activity changes. The potential directional alterations in functional connectivity within the primary visual cortex (V1) in RD patients remain to be elucidated.Methods: In this study, we employed seed-based functional connectivity analysis along with Granger causality analysis to examine the directional alterations in dynamic functional connectivity (dFC) within the V1 region of patients diagnosed with RD. Finally, a support vector machine algorithm was utilized to classify patients with RD and healthy controls (HCs).Results: RD patients exhibited heightened dynamic functional connectivity (dFC) and dynamic effective connectivity (dEC) between the Visual Network (VN) and default mode network (DMN), as well as within the VN, compared to HCs. Conversely, dFC between VN and auditory network (AN) decreased, and dEC between VN and sensorimotor network (SMN) significantly reduced. In state 4, RD patients had higher frequency. Notably, variations in dFC originating from the left V1 region proved diagnostically effective, achieving an AUC of 0.786.Conclusion: This study reveals significant alterations in the connectivity between the VN and the default mode network in patients with RD. These changes may disrupt visual information processing and higher cognitive integration in RD patients. Additionally, alterations in the left V1 region and whole-brain dFC show promising potential in aiding the diagnosis of RD. These findings offer valuable insights into the neural mechanisms underlying visual and cognitive impairments associated with RD.","PeriodicalId":19213,"journal":{"name":"Neuroreport","volume":" ","pages":"1071-1081"},"PeriodicalIF":1.6,"publicationDate":"2024-12-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142471000","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Electroacupuncture alleviated chronic cerebral hypoperfusion damages via targeting aquaporin 4 to prevent amyloid-beta accumulation. 电针通过靶向水蒸发蛋白4防止淀粉样蛋白-β积聚减轻慢性脑灌注不足的损害

IF 1.6 4区医学

Neuroreport Pub Date : 2024-11-12 DOI: 10.1097/WNR.0000000000002114

Ying Zhang, Lei Wu, Kelin He, Yingying Cheng, Lu Li, Dexiong Han

{"title":"Electroacupuncture alleviated chronic cerebral hypoperfusion damages via targeting aquaporin 4 to prevent amyloid-beta accumulation.","authors":"Ying Zhang, Lei Wu, Kelin He, Yingying Cheng, Lu Li, Dexiong Han","doi":"10.1097/WNR.0000000000002114","DOIUrl":"https://doi.org/10.1097/WNR.0000000000002114","url":null,"abstract":"The present study aimed to investigate the therapeutic effects of electroacupuncture (EA) on chronic cerebral hypoperfusion (CCH). We first applied the Morris water maze approach to determine the effects of EA and TGN-020 [an inhibitor of aquaporin 4 (AQP4)] on the learning and memory ability of CCH rats. The hematoxylin and eosin, and Nissl staining were further used to investigate the effects of EA and TGN-020 on the neuropathological changes of the dentate gyrus. Next, the ELISA kits were adopted to determine the effects of EA and TGN-020 on the content of amyloid-beta (Aβ) in the cerebrospinal fluid of CCH rats. Finally, we respectively employed technologies of immunohistochemical staining, quantitative real-time PCR, and Western blot to further explore the effects of EA and TGN-020 on the mRNA expression level of amyloid precursor protein (APP) and AQP4 as well as the protein expression level of Aβ1-42 and AQP4 in the dentate gyrus of CCH rats. Our results indicated that EA not only enhanced the learning and memory abilities of CCH rats but also improved the neuropathological damages of CCH rats by upregulating the mRNA and protein expression level of AQP4 to reduce the accumulation of Aβ, especially for the reduction of the mRNA expression level of APP and the protein expression level of Aβ1-42, but TGN-020 effectively reversed the therapeutic effects mentioned above of EA. In summary, we proved that EA, as the activator of AQP4, prevents the accumulation of Aβ during the treatment of CCH.","PeriodicalId":19213,"journal":{"name":"Neuroreport","volume":" ","pages":""},"PeriodicalIF":1.6,"publicationDate":"2024-11-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142624955","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Altered static brain activity and functional connectivity after heat stroke. 中暑后大脑静态活动和功能连接的改变。

IF 1.6 4区医学

Neuroreport Pub Date : 2024-11-06 Epub Date: 2024-09-19 DOI: 10.1097/WNR.0000000000002096

Ting Yang, Xinyu Hou, Xu Li, Xiaoxin Chen, Zhengwei Qu, Wenzheng Zhang, Maoyang Wang, Jun Li

{"title":"Altered static brain activity and functional connectivity after heat stroke.","authors":"Ting Yang, Xinyu Hou, Xu Li, Xiaoxin Chen, Zhengwei Qu, Wenzheng Zhang, Maoyang Wang, Jun Li","doi":"10.1097/WNR.0000000000002096","DOIUrl":"10.1097/WNR.0000000000002096","url":null,"abstract":"This study aimed to investigate the alteration of brain function based on resting-state functional MRI in patients after heat stroke. This study included 10 cases of patients after heat stroke and 10 cases of healthy controls. Abnormal brain function was calculated using amplitude of low-frequency fluctuations (ALFF) and degree centrality analysis, as well as functional connectivity analysis based on regions of interest (ROI). Correlation analyses were performed to evaluate the association between brain function changes and clinical scales. Combining ALFF and degree centrality results, the decreased brain regions included the left cuneus and the right angular gyrus, while the increased brain regions included the right cerebellar_Crus1. Using the left cuneus with significant differences in ALFF and degree centrality as ROI, the functional connectivity results revealed decreased brain regions including bilateral lingual gyrus, bilateral postcentral cingulate gyrus, and left precentral gyrus. The degree centrality value of the right cerebellar_Crus1 was positively correlated with glasgow coma scale (GCS) scores ( r = 0.726, P = 0.027), and the functional connectivity value of the right posterior cingulate gyrus was positively correlated with GCS scores ( r = 0.717, P = 0.030). Heat stroke patients exhibit abnormal activity in multiple brain regions, which has important clinical significance for evaluating the severity of the disease.","PeriodicalId":19213,"journal":{"name":"Neuroreport","volume":" ","pages":"1035-1040"},"PeriodicalIF":1.6,"publicationDate":"2024-11-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142292214","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

pRb phosphorylation regulates the proliferation of supporting cells in gentamicin-damaged neonatal avian utricle: Erratum. pRb磷酸化调控庆大霉素损伤的新生禽类胞器支持细胞的增殖勘误。

IF 1.6 4区医学

Neuroreport Pub Date : 2024-11-06 Epub Date: 2024-09-25 DOI: 10.1097/WNR.0000000000002102

引用次数: 0

Can minor trauma cause asymptomatic cervical spinal cord compression leading to severe cervical spinal cord injury in rats? 轻微外伤会导致大鼠无症状性颈椎脊髓压迫，从而导致严重的颈椎脊髓损伤吗？

IF 1.6 4区医学

Neuroreport Pub Date : 2024-11-06 DOI: 10.1097/WNR.0000000000002113

Luchun Xu, Ting Wang, Zheng Cao, Yushan Gao, Guozheng Jiang, Yukun Ma, Jiawei Song, Yongdong Yang, Xing Yu

{"title":"Can minor trauma cause asymptomatic cervical spinal cord compression leading to severe cervical spinal cord injury in rats?","authors":"Luchun Xu, Ting Wang, Zheng Cao, Yushan Gao, Guozheng Jiang, Yukun Ma, Jiawei Song, Yongdong Yang, Xing Yu","doi":"10.1097/WNR.0000000000002113","DOIUrl":"https://doi.org/10.1097/WNR.0000000000002113","url":null,"abstract":"The study aimed to determine whether asymptomatic rats with cervical spinal cord compression (CSCC) experience more severe cervical spinal cord injury (SCI) compared with rats without CSCC under the same degree of minor trauma. Four weeks after the polyvinyl alcohol-polyacrylamide hydrogel was implanted into the C5 vertebral canal, asymptomatic rats were selected based on locomotor function score. Mild cervical SCI was subsequently established based on CSCC. The motor function, morphology, neuron loss, myelin destruction, nerve cell apoptosis, microglia activation, and neuroinflammation were evaluated after SCI. Under the same injury conditions, rats in the CSCC group exhibited more severe motor dysfunction compared with those without CSCC. Similarly, asymptomatic CSCC rats showed significant damage to spinal cord tissue, neurons, and myelin. Finally, compared with rats without CSCC, asymptomatic CSCC rats experienced increased nerve cell apoptosis, microglial activation, and neuroinflammation following the same SCI. In asymptomatic CSCC rats, the same degree of minor trauma resulted in more severe cervical SCI compared with rats without CSCC. This was evidenced by increased nerve cell apoptosis, microglial activation, neuron death, myelin destruction, and a strong neuroinflammatory response, leading to severe motor dysfunction.","PeriodicalId":19213,"journal":{"name":"Neuroreport","volume":" ","pages":""},"PeriodicalIF":1.6,"publicationDate":"2024-11-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142605777","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Adipose-derived stem cell transplantation enhances spinal cord regeneration by upregulating PGRN expression. 脂肪源性干细胞移植通过上调PGRN的表达增强脊髓再生。

IF 1.6 4区医学

Neuroreport Pub Date : 2024-11-06 Epub Date: 2024-09-12 DOI: 10.1097/WNR.0000000000002091

Qiongchi Zhang, Jingtao Wu, Dong Guo, Ning Ji, Weidong Liu, Xinyu Li, Hao Liu, Chengyi Zhang, Minchao Zhao, Haopeng Li, Hongxu Jin, Su'e Chang, Dong Wang

{"title":"Adipose-derived stem cell transplantation enhances spinal cord regeneration by upregulating PGRN expression.","authors":"Qiongchi Zhang, Jingtao Wu, Dong Guo, Ning Ji, Weidong Liu, Xinyu Li, Hao Liu, Chengyi Zhang, Minchao Zhao, Haopeng Li, Hongxu Jin, Su'e Chang, Dong Wang","doi":"10.1097/WNR.0000000000002091","DOIUrl":"10.1097/WNR.0000000000002091","url":null,"abstract":"This study aims to investigate the effect of adipose-derived stem cells (ADSCs) transplantation on progranulin (PGRN) expression and functional recovery in rats with spinal cord injury (SCI). ADSCs were isolated from the inguinal adipose tissue of rats. A SCI model was created, and ADSCs were injected into the injured area. Various techniques were used to assess the effects of ADSCs transplantation, including hematoxylin-eosin staining, Masson staining, immunofluorescence staining, electron microscopy, MRI, and motor function assessment. The potential mechanisms of ADSC transplantation were investigated using gene expression analysis and protein analysis. Finally, the safety of this therapy was evaluated through hematoxylin-eosin staining and indicators of liver and kidney damage in serum. PGRN expression increased in the injured spinal cord, and ADSCs transplantation further enhanced PGRN levels. The group that received ADSCs transplantation showed reduced inflammation, decreased scar formation, increased nerve regeneration, and faster recovery of bladder function. Importantly, motor function significantly improved in the ADSC transplantation group. ADSCs transplantation enhances functional regeneration in SCI by upregulating PGRN expression, reducing inflammation and scar formation, and promoting nerve regeneration and myelin repair. These findings suggest that ADSC transplantation is a potential therapy for SCI.","PeriodicalId":19213,"journal":{"name":"Neuroreport","volume":" ","pages":"1019-1029"},"PeriodicalIF":1.6,"publicationDate":"2024-11-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142292213","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A revisit to interactions of stimulus quality and semantic context on N400 in visual word recognition. 重新审视刺激质量和语义语境对视觉单词识别 N400 的交互作用。

IF 1.6 4区医学

Neuroreport Pub Date : 2024-11-06 Epub Date: 2024-09-25 DOI: 10.1097/WNR.0000000000002095

Rongmin Xiong, Yong Zhang, Wenyi Hu, Shixue Zhu, Zhongxuan Huang, Quanhong Wang

{"title":"A revisit to interactions of stimulus quality and semantic context on N400 in visual word recognition.","authors":"Rongmin Xiong, Yong Zhang, Wenyi Hu, Shixue Zhu, Zhongxuan Huang, Quanhong Wang","doi":"10.1097/WNR.0000000000002095","DOIUrl":"10.1097/WNR.0000000000002095","url":null,"abstract":"Much behavioral research has revealed interactive effects between stimulus quality and semantic priming in visual word recognition, practically in favor of the interactive activation model. However, the limited number of event-related brain potential (ERP) studies have yielded inconsistent results considering this interaction's impact on N400 amplitude. The current ERP study aimed to examine whether the joint effects of stimulus quality and semantic priming were specific to the lexical decision task. We used both behavioral measures and ERP recordings to evaluate the joint effects of stimulus degradation (i.e. highly vs. slightly degraded) and semantic priming (i.e. semantically related vs. unrelated) in a lexical decision task involving visual recognition of Chinese characters. The results showed significant degradation-by-priming interactions on response times and N400 amplitude ( P < 0.05), with larger semantic priming effects on slightly degraded targets. These converging behavioral and electrophysiological findings provide evidence in accordance with the interactive activation models of visual word recognition, in which the early-stage visual processing (i.e. degradation) cascades into the later-stage semantic processing (i.e. priming), thus yielding interactions observed in N400 amplitude.","PeriodicalId":19213,"journal":{"name":"Neuroreport","volume":" ","pages":"1030-1034"},"PeriodicalIF":1.6,"publicationDate":"2024-11-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142154692","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Specific mode electroacupuncture stimulation opens the blood-brain barrier of the infarcted border zone in rats during MCAO/R recovery via modulation of tight junction protein expression by VEGFA and NF-κB. 特定模式电针刺激通过调节 VEGFA 和 NF-κB 的紧密连接蛋白表达，在 MCAO/R 恢复期打开大鼠梗死边界区的血脑屏障。

IF 1.6 4区医学

Neuroreport Pub Date : 2024-11-06 Epub Date: 2024-09-19 DOI: 10.1097/WNR.0000000000002098

Kecheng Qian, Mengyuan Dai, Lin Gan, Qinyu Ye, Xingying Wu, Tianyu Qian, Congcong Ma, Xianming Lin

{"title":"Specific mode electroacupuncture stimulation opens the blood-brain barrier of the infarcted border zone in rats during MCAO/R recovery via modulation of tight junction protein expression by VEGFA and NF-κB.","authors":"Kecheng Qian, Mengyuan Dai, Lin Gan, Qinyu Ye, Xingying Wu, Tianyu Qian, Congcong Ma, Xianming Lin","doi":"10.1097/WNR.0000000000002098","DOIUrl":"10.1097/WNR.0000000000002098","url":null,"abstract":"The blood-brain barrier (BBB) strictly limits the entry of most exogenous therapeutic drugs into the brain, which brings great challenges to the drug treatment of refractory central diseases, including the treatment of ischemic stroke. Our previous studies have shown that specific mode electroacupuncture stimulation (SMES) can temporarily open the BBB, but with the mechanisms largely unknown. This study explored whether SMES opens the BBB in the infarcted border zone of rats during middle cerebral artery occlusion/reperfusion recovery, and whether this is related to p65 or vascular endothelial growth factor A (VEGFA) modulation of tight junction protein expression through in vivo and in vitro studies. Evans blue, FITC-dextran, mouse-derived nerve growth factor (NGF), and transendothelial electrical resistance values were used to evaluate the permeability of the BBB. Additionally, microvascular endothelial cells and astrocytes were utilized for in vitro study. Immunofluorescence, immunohistochemistry, western blot, and ELISA were employed to assess related protein expression. SMES significantly increased vascular permeability for Evans blue and NGF in the infarcted border zone, and increased the expression of VEGFA by activating p-p65, thereby reducing the expression of tight junction proteins Occludin and ZO-1. Correspondingly, oxygen glucose deprivation/reoxygenation activated p-p65 in and induced VEGFA secretion from astrocytes in vitro. Their conditioned medium reduced the expression of Occludin in bEnd.3 cells and increased the permeability of FITC-dextran. The mechanism of SMES opening infarcted border zone BBB is partly related to its actions on p65, VEGFA, and tight junction proteins.","PeriodicalId":19213,"journal":{"name":"Neuroreport","volume":" ","pages":"1052-1060"},"PeriodicalIF":1.6,"publicationDate":"2024-11-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142292216","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0