Neuroreport最新文献_第2页

Lipid droplets deposition in perihematoma tissue is associated with neurological dysfunction after intracerebral hemorrhage.

IF 1.6 4区医学

Neuroreport Pub Date : 2025-03-19 Epub Date: 2025-02-26 DOI: 10.1097/WNR.0000000000002136

Zhangze Wu, Quan Zhao, Ziqi Hu, Dongsheng Jiao

{"title":"Lipid droplets deposition in perihematoma tissue is associated with neurological dysfunction after intracerebral hemorrhage.","authors":"Zhangze Wu, Quan Zhao, Ziqi Hu, Dongsheng Jiao","doi":"10.1097/WNR.0000000000002136","DOIUrl":"10.1097/WNR.0000000000002136","url":null,"abstract":"Secondary brain injury following intracerebral hemorrhage (ICH) significantly reduces patients' quality of life due to impaired neurological function. Lipid droplets are implicated in secondary brain injury in various central nervous system diseases. Thus, the role and mechanisms of lipid droplets in secondary brain injury post-ICH require further investigation. We analyzed the changes of genes related to lipid metabolism in brain tissue of ICH mice. Lipid droplets around the hematoma were detected by BODIPY staining. Mice received intraperitoneal injections of Triacsin C (10 mg/kg, once daily) after ICH. Subsequently, neuronal damage was evaluated using TUNEL and Nissl staining, and ethological tests assessed sensorimotor function. After ICH, notable changes occurred in lipid metabolism pathways and genes (Plin2, Ucp2, Apoe), and a large number of lipid droplets accumulated around the hematoma. Triacsin C significantly reduced lipid droplets deposition, decreased neuronal damage, and improved sensory and motor functions. Peripheral administration to prevent lipid droplets formation can greatly reduce nerve damage and enhance nerve function. Our findings indicate that targeting lipid droplets could be a promising treatment for ICH.","PeriodicalId":19213,"journal":{"name":"Neuroreport","volume":" ","pages":"239-246"},"PeriodicalIF":1.6,"publicationDate":"2025-03-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143458784","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Electroacupuncture preconditioning alleviates cortical neuronal injury in cerebral ischemia-reperfusion by rebalancing HES1 and NF-κB expression.

IF 1.6 4区医学

Neuroreport Pub Date : 2025-03-05 Epub Date: 2025-02-12 DOI: 10.1097/WNR.0000000000002142

Yegui Zhang, Jisheng Wang, Shi Huang, Min Liu, Yingdi Zhao, Zhiliang Xu, Xiuling Zhu

{"title":"Electroacupuncture preconditioning alleviates cortical neuronal injury in cerebral ischemia-reperfusion by rebalancing HES1 and NF-κB expression.","authors":"Yegui Zhang, Jisheng Wang, Shi Huang, Min Liu, Yingdi Zhao, Zhiliang Xu, Xiuling Zhu","doi":"10.1097/WNR.0000000000002142","DOIUrl":"10.1097/WNR.0000000000002142","url":null,"abstract":"The roles of Hairy Enhancer of Split 1 (HES1) and nuclear factor kappa B (NF-κB) in neuronal injury following cerebral ischemia have been well established. However, the relationship between electroacupuncture preconditioning-induced neuroprotection and these signaling pathways remains unclear. This study aimed to investigate the effects of electroacupuncture pretreatment on the expression of HES1 and NF-κB in the cortex of rats subjected to cerebral ischemia/reperfusion and to explore the underlying mechanisms. A total of 36 male Sprague-Dawley rats were randomized into three groups: sham, model, and electroacupuncture pretreatment ( n = 12 per group). Cerebral ischemia/reperfusion was induced using the middle cerebral artery occlusion (MCAO) method. Rats in the electroacupuncture group received daily electroacupuncture stimulation at 'Baihui' and 'Dazhui' acupoints 1 week prior to MCAO. Neurological function scores, 2,3,5-triphenyltetrazolium chloride staining for cerebral infarction, Nissl staining for neuronal cell survival, and immunohistochemical detection of HES1 and NF-κB expression in the cerebral cortex were assessed. Compared to the sham group, the model group exhibited significant neurological deficits, extensive cerebral infarction, decreased neuronal survival, and increased expression of HES1 and NF-κB ( P < 0.01). In contrast, electroacupuncture pretreatment significantly improved neurological function scores ( P < 0.01), reduced cerebral infarction ( P < 0.01), increased neuronal survival ( P < 0.05), and decreased the expression of HES1 and NF-κB ( P < 0.01). These findings suggest that electroacupuncture preconditioning may mitigate brain injury in cerebral ischemia/reperfusion rats by downregulating the expression of HES1 and NF-κB, thereby reducing inflammatory damage and enhancing cerebral ischemia tolerance.","PeriodicalId":19213,"journal":{"name":"Neuroreport","volume":" ","pages":"230-237"},"PeriodicalIF":1.6,"publicationDate":"2025-03-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143458778","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Gamma-aminobutyric acid and glutamate/glutamine associations with tinnitus and hearing loss.

IF 1.6 4区医学

Neuroreport Pub Date : 2025-03-05 Epub Date: 2025-02-12 DOI: 10.1097/WNR.0000000000002137

Rafay Ali Khan, Zepeng Wang, Ruiyang Zhao, Gibbeum Kim, Fan Lam, Fatima Tazeena Husain

{"title":"Gamma-aminobutyric acid and glutamate/glutamine associations with tinnitus and hearing loss.","authors":"Rafay Ali Khan, Zepeng Wang, Ruiyang Zhao, Gibbeum Kim, Fan Lam, Fatima Tazeena Husain","doi":"10.1097/WNR.0000000000002137","DOIUrl":"10.1097/WNR.0000000000002137","url":null,"abstract":"The present study aimed to evaluate the association between concentrations of the metabolites gamma-aminobutyric acid (GABA) and glutamate/glutamine (Glx), which have predominantly inhibitory and excitatory effects on neural function, respectively, in adults with tinnitus and hearing loss, those with only hearing loss, and controls with neither condition. Metabolite concentrations in all three participant groups were assessed via magnetic resonance spectroscopic imaging in auditory and fronto-parietal regions. The concentration of a third metabolite, creatine (Cre) was also acquired. Ratios of GABA/Cre, Glx/Cre, and Glx/GABA were compared across six manually delineated regions of interest (ROIs). Neither GABA/Cre nor Glx/Cre showed significant group differences in any of the six ROIs. For the Glx/GABA ratio, group-level differences were seen only in the right auditory cortex, where the control group had a significantly larger ratio than the group with tinnitus and hearing loss. While results largely did not replicate previous human work in this area, we cannot exclude the possibility of a neurochemical mechanism underlying any causal relationship between hearing loss and tinnitus, particularly given the finding of altered balance in excitatory/inhibitory metabolites in the right auditory cortex. In the context of previous work, the right auditory cortex is highlighted as a particular region of interest for further investigation. Methodological differences in human studies and inconsistent findings in animal studies have thus far impeded the field's ability to gain direct insight into the relationship between tinnitus and hearing loss, and so we make some suggestions to help design future studies.","PeriodicalId":19213,"journal":{"name":"Neuroreport","volume":" ","pages":"196-201"},"PeriodicalIF":1.6,"publicationDate":"2025-03-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143458781","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Dynamic and static brain functional abnormalities in autism patients at different developmental stages.

IF 1.6 4区医学

Neuroreport Pub Date : 2025-03-05 Epub Date: 2025-02-12 DOI: 10.1097/WNR.0000000000002139

Haonan Li, Mingxing Han, Shaoting Tang, Yaqian Yang

{"title":"Dynamic and static brain functional abnormalities in autism patients at different developmental stages.","authors":"Haonan Li, Mingxing Han, Shaoting Tang, Yaqian Yang","doi":"10.1097/WNR.0000000000002139","DOIUrl":"10.1097/WNR.0000000000002139","url":null,"abstract":"To date, most studies on autism spectrum disorder (ASD) have focused on specific age ranges, while the mechanisms underlying the entire developmental process of autism patients remain unclear. The aim of this study was to investigate the alterations in brain function in autistic individuals at different developmental stages by resting-state functional MRI (rs-fMRI). We obtained rs-fMRI data from 173 ASD and 178 typical development (TD) individuals in Autism Brain Imaging Data Exchange, spanning child, adolescent, and adult groups. We characterized local brain activity using the amplitude of low-frequency fluctuations (ALFFs), regional homogeneity (ReHo), dynamic ALFF (dALFF), and dynamic ReHo (dReHo) metrics. Pearson correlation analyses were conducted on relationships between Autism Diagnostic Observation Schedule scores and activity measures in abnormal brain regions. We found abnormal ALFF values in the medial and lateral orbitofrontal gyrus and right insula cortex with ASD compared with the TD group. In addition, compared with adolescents with ASD, we found that adults with ASD exhibited an increase in dReHo values in the posterior lateral frontal lobe. We also found that changes in ALFF were associated with the severity of autism. We found abnormal activity in multiple brain regions in individuals with autism and correlated it with clinical characteristics. Our results may provide some help for further exploring the age-related neurobiological mechanisms of ASD patients.","PeriodicalId":19213,"journal":{"name":"Neuroreport","volume":" ","pages":"202-210"},"PeriodicalIF":1.6,"publicationDate":"2025-03-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11867798/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143458777","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Enhanced cerebellar climbing fiber-Purkinje cell synaptic transmission via corticotropin-releasing factor receptor 2 during the chronic phase of spinal cord injury mice.

IF 1.6 4区医学

Neuroreport Pub Date : 2025-03-05 Epub Date: 2025-02-12 DOI: 10.1097/WNR.0000000000002141

Wen-Cai Weng, Ying-Han Xu, De-Lai Qiu, Chun-Ping Chu

{"title":"Enhanced cerebellar climbing fiber-Purkinje cell synaptic transmission via corticotropin-releasing factor receptor 2 during the chronic phase of spinal cord injury mice.","authors":"Wen-Cai Weng, Ying-Han Xu, De-Lai Qiu, Chun-Ping Chu","doi":"10.1097/WNR.0000000000002141","DOIUrl":"10.1097/WNR.0000000000002141","url":null,"abstract":"Spinal cord injury (SCI) causes interruption of external information input from the spinal cord to the cerebellum. We here investigated the effect of SCI on mouse cerebellar climbing fiber-Purkinje cell (CF-PC) synaptic transmission. The SCI was caused at T10 using 6-week-old ICR mice. Mice recovered 4 weeks after surgery, the spontaneous complex spike (CS) activity of PC was recorded using cell-attached recording and whole-cell recording method in urethane-anesthetized mice cerebellar Crus II. The CF-PC excitatory postsynaptic currents (EPSCs) were evoked by paired electrical stimulation of CF in cerebellar slices to evaluate the changes of CF-PC synaptic transmission and paired-pulse ratio (PPR). The results showed that the number of spikelets, duration of spontaneous CS, and pause of simple spike firing were significantly increased in SCI than that in sham group. Application of a nonselective corticotropin-releasing factor receptor (CRF-R) antagonist significantly decreased spontaneous CS activity in SCI group but not in sham group. The enhanced CS activity in SCI mice was significantly decreased by a selective CRF-R2 antagonist but not a specific CRF-R1 antagonist. The amplitude of CF-PC EPSC1 was large accompanied by a lower PPR in SCI group than that in sham group. Blockade of CRF-R2 antagonist significantly decreased the amplitude of EPSC1 and increased PPR in SCI group. SCI induces enhancement of the spontaneous CS activity and CF-PC synaptic transmission via CRF-R2 in mouse cerebellar cortex, which suggests that remodeling of CF-PC synaptic transmission occurred in cerebellar cortex after SCI.","PeriodicalId":19213,"journal":{"name":"Neuroreport","volume":" ","pages":"223-229"},"PeriodicalIF":1.6,"publicationDate":"2025-03-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143458780","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

CB1 receptor coupling to extracellular regulated kinase via multiple Gαi/o isoforms.

IF 1.6 4区医学

Neuroreport Pub Date : 2025-03-05 Epub Date: 2025-02-12 DOI: 10.1097/WNR.0000000000002138

Boyd R Rorabaugh, Daniel J Morgan

{"title":"CB1 receptor coupling to extracellular regulated kinase via multiple Gαi/o isoforms.","authors":"Boyd R Rorabaugh, Daniel J Morgan","doi":"10.1097/WNR.0000000000002138","DOIUrl":"10.1097/WNR.0000000000002138","url":null,"abstract":"Cannabinoid type 1 receptors (CB 1 Rs) play important roles in regulating neurotransmitter release, synaptic plasticity, cell differentiation, and survival. CB 1 R is coupled via pertussis toxin (PTX)-sensitive Gαi/o proteins to the activation of extracellular regulated kinase (ERK) signaling. However, there are multiple Gαi/o isoforms, and it is unknown which of these isoforms is responsible for CB 1 R-induced phosphorylation of ERK. The purpose of this study was to determine which Gαi/o isoform(s) couple CB 1 R to ERK phosphorylation. HEK293 cells stably expressing the mouse CB 1 R (CB 1 R-HEK cells) were transfected with either pcDNA3.1 or pcDNA3.1 encoding PTX-insensitive mutants of Gαo, Gαi1, Gαi2, or Gαi3. PTX was used to inactivate endogenous Gαi/o isoforms before cells were treated with vehicle, delta-9-tetrahydrocannabinol (∆ 9 -THC), or CP55940 and ERK phosphorylation was measured by western blotting. CP55940 induced robust phosphorylation of ERK in cells transfected with vector alone. This effect was completely abolished by PTX treatment. CP55940-induced ERK phosphorylation was rescued by expression of PTX-insensitive forms of Gαo, Gαi1, Gαi2, or Gαi3, indicating that the CB1 receptor can couple to ERK phosphorylation through each of these Gαi/o isoforms. Consistent with its actions as a partial agonist, ∆ 9 -THC induced nominal (two to four-fold) increases in ERK phosphorylation that did not reach statistical significance except in cells transfected with PTX-insensitive Gαi3. These data demonstrate that CB 1 R can couple to ERK phosphorylation through Gαo, Gαi1, Gαi2, or Gαi3 when stimulated with CP55940 (full agonist). However, ∆ 9 -THC (partial agonist)-induced ERK activation might require high levels of Gαi3 expression.","PeriodicalId":19213,"journal":{"name":"Neuroreport","volume":" ","pages":"191-195"},"PeriodicalIF":1.6,"publicationDate":"2025-03-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11952984/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143458776","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Time-course changes in videofluoroscopic findings during developmental and aging stages in mice.

IF 1.6 4区医学

Neuroreport Pub Date : 2025-03-05 Epub Date: 2025-02-12 DOI: 10.1097/WNR.0000000000002140

Hiyori Takahashi, Jun Suzuki, Ryoukichi Ikeda, Takuma Hisaoka, Yasutoshi Koshiba, Jun Ohta, Ai Hirano-Kawamoto, Yukio Katori

{"title":"Time-course changes in videofluoroscopic findings during developmental and aging stages in mice.","authors":"Hiyori Takahashi, Jun Suzuki, Ryoukichi Ikeda, Takuma Hisaoka, Yasutoshi Koshiba, Jun Ohta, Ai Hirano-Kawamoto, Yukio Katori","doi":"10.1097/WNR.0000000000002140","DOIUrl":"10.1097/WNR.0000000000002140","url":null,"abstract":"Videofluoroscopic swallow study methodology in mice has recently been established; however, details of developmental and age-related changes remain unclear. This study aimed to reveal the changes in swallowing associated with the entire lifespan of mice using the videofluoroscopic swallow study methodology. We tested six age groups of male C57BL/6J mice (4, 8, 16, 54, 77, and 104 weeks old; n = 5 for each group, respectively). The videofluoroscopic swallow study analyzed the bolus area, pharyngeal transit time, interswallow interval, lick-swallow ratio, and lick rate. Significant age differences were found in the bolus area and lick rate. The bolus area increased significantly as the mice grew older. There was a significant increase in the lick rate from 4 to 8 weeks ( P < 0.001) and then a significant decrease from 8 to 104 weeks ( P < 0.01). No significant difference was observed between the various stages in the lick-swallow ratio, interswallow interval, or pharyngeal transit time. There was a significant positive correlation between the body weight and bolus area. This study revealed the detailed changes in swallowing at each life stage from swallowing maturation to old age in the mice. Our research may serve as a guide for future studies on swallowing in humans.","PeriodicalId":19213,"journal":{"name":"Neuroreport","volume":" ","pages":"211-216"},"PeriodicalIF":1.6,"publicationDate":"2025-03-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143458805","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The effects of test on associative memory depend on semantic relevancy levels: evidence of event-related potentials.

IF 1.6 4区医学

Neuroreport Pub Date : 2025-03-05 Epub Date: 2025-02-12 DOI: 10.1097/WNR.0000000000002143

Wenting Geng, Yan Wang, Xintong Liu, Chunping Yan, Yunbing Zhang, Tian Gao

{"title":"The effects of test on associative memory depend on semantic relevancy levels: evidence of event-related potentials.","authors":"Wenting Geng, Yan Wang, Xintong Liu, Chunping Yan, Yunbing Zhang, Tian Gao","doi":"10.1097/WNR.0000000000002143","DOIUrl":"10.1097/WNR.0000000000002143","url":null,"abstract":"This study aims to investigate how the level of semantic relevance influences the effects of test on associative memory through bottom-up cognitive processes. This study manipulated the levels of semantic relevancy using synonym and nonsynonym pairs in a three-phase testing effect paradigm (an initial encoding phase, a cued-recall test as retrieval practice, and a final test phase) to explore the effects of testing effect and semantic relevancy on associative recognition by bottom-up processes using the event-related potential (ERP). The behavioral results suggested that the test practice (relative to the restudy) significantly facilitated memory retrieval in high semantic relevancy pairs during the final test phase. The ERP results showed that the testing effect tended toward right laterality over time in the retrieval practice phase. Moreover, test practice (relative to the restudy) significantly enhanced FN400 for high semantic relevancy and intact pairs and significantly enhanced FN400 and late positive complex for high semantic relevancy and rearranged pairs in the final test phase. These results suggest that the test practice provided a greater boost in memory encoding and retrieval for high semantic relevancy items. Specifically, the effects of test on associative memory is modulated by the level of semantic relevancy.","PeriodicalId":19213,"journal":{"name":"Neuroreport","volume":" ","pages":"217-222"},"PeriodicalIF":1.6,"publicationDate":"2025-03-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143458790","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Diabetes may contribute to cognitive impairment in Parkinson's disease via damaging white matter tracts. 糖尿病可能通过破坏白质束导致帕金森病的认知障碍。

IF 1.6 4区医学

Neuroreport Pub Date : 2025-02-05 Epub Date: 2024-12-02 DOI: 10.1097/WNR.0000000000002123

Zhihui Liu, Jiahao Wei, Huahua Su, Yuchen She, Junyi Wang, Ying Liu, Lijuan Mo, Changhong Tan, Xi Liu, Lifen Chen

{"title":"Diabetes may contribute to cognitive impairment in Parkinson's disease via damaging white matter tracts.","authors":"Zhihui Liu, Jiahao Wei, Huahua Su, Yuchen She, Junyi Wang, Ying Liu, Lijuan Mo, Changhong Tan, Xi Liu, Lifen Chen","doi":"10.1097/WNR.0000000000002123","DOIUrl":"10.1097/WNR.0000000000002123","url":null,"abstract":"Parkinson's disease with dementia (PDD) severely affects the quality of life of patients with Parkinson's disease (PD) in the later stages. Recently, PD patients with diabetes were found to have a higher risk of cognitive decline and developing dementia with a faster progression, but the underlying mechanism remains unclear. Diabetes-related white matter damage may partially explain the mechanism by which diabetes participates in PDD. Seventy PD patients were included. PD patients underwent diffusion tensor imaging from The Second Affiliated Hospital of Chongqing Medical University were collected and were divided into four groups: PD with diabetes without dementia, PD with dementia without diabetes, PD without dementia and diabetes. Tract-based spatial statistics analysis and region-of-interest-based analysis were performed. Factorial analysis with diabetes and dementia taken as the main effects was performed, and the differences between the white matter fibers of PD patients from the four groups were also analyzed. The interaction between diabetes and dementia in the damage of white matter in PD patients was also analyzed. We found that both diabetes and dementia were found to be related to the damage in internal capsule, corona radiata, and thalamic radiation of the PD patients. There is an interaction between diabetes and dementia in the white matter damage of PD patients. Both diabetes and dementia were found to be related to the damage in internal capsule, corona radiata, and thalamic radiation of the PD patients. Diabetes may participate in cognitive decline in PD patients via damaging cognition-related white matter tracts.","PeriodicalId":19213,"journal":{"name":"Neuroreport","volume":" ","pages":"71-80"},"PeriodicalIF":1.6,"publicationDate":"2025-02-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142813803","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

TRIM37 exacerbates cerebral ischemic injury by regulating the PPARγ/NF-κB pathway. TRIM37通过调控PPARγ/NF-κB通路加重脑缺血损伤。

IF 1.6 4区医学

Neuroreport Pub Date : 2025-02-05 Epub Date: 2024-12-06 DOI: 10.1097/WNR.0000000000002126

Guixin Shi, Linyan Fu, Hua Xiao, Fang Cao

{"title":"TRIM37 exacerbates cerebral ischemic injury by regulating the PPARγ/NF-κB pathway.","authors":"Guixin Shi, Linyan Fu, Hua Xiao, Fang Cao","doi":"10.1097/WNR.0000000000002126","DOIUrl":"10.1097/WNR.0000000000002126","url":null,"abstract":"Ischemic stroke is the primary cause of mortality for individuals with disability worldwide. Tripartite motif 37 (TRIM37) plays multiple regulatory roles in various cellular processes. Our research aimed to investigate the effects of TRIM37 on the progression of ischemic stroke and its related mechanisms. Primary rat brain microvascular endothelial cells (BMECs) were treated with oxygen-glucose deprivation and reoxygenation (OGD/R) and then transduced with pShuttle-H1-TRIM37 shRNA plasmid, pShuttle-CMV-TRIM37 plasmid, or corresponding negative controls. The effects of TRIM37 were also explored in middle cerebral artery occlusion surgery-induced rat brain damage in vivo . Factor VIII staining showed the successful isolation of the primary BMECs. The OGD/R procedure significantly inhibited the cell viability and upregulated the TRIM37 expression in a time-dependent manner. In addition, OGD/R evidently increased the cell permeability, elevated the tumor necrosis factor alpha and intercellular adhesion molecule 1 levels, and upregulated the nuclear expression of nuclear factor (NF)-κB, but downregulated the peroxisome proliferator-activated receptors γ (PPARγ), zonula occludens-1, and cytoplasmic NF-κB expressions, which were reversed by TRIM37 knockdown. Furthermore, TRIM37 interacted with PPARγ and promoted its ubiquitination. The effects on cell permeability and inflammation induced by TRIM37 overexpression were reversed by PPARγ agonist. TRIM37 knockdown also inhibited middle cerebral artery occlusion-induced rat brain damage in vitro . TRIM37 may be a potential therapeutic target for ischemic stroke, and the knockdown of TRIM37 may improve brain injury by regulating the PPARγ/NF-κB pathway to modulate the inflammatory response.","PeriodicalId":19213,"journal":{"name":"Neuroreport","volume":" ","pages":"105-115"},"PeriodicalIF":1.6,"publicationDate":"2025-02-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142813888","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0