Neuroreport最新文献_第2页

Effect of deep brain stimulation for lateral hypothalamic area on memory decline and hippocampal neurofilaments expression dysfunctions in aged rats. 下丘脑外侧区深部脑刺激对老年大鼠记忆衰退和海马神经丝表达功能障碍的影响。

IF 1.6 4区医学

Neuroreport Pub Date : 2025-05-14 Epub Date: 2025-04-11 DOI: 10.1097/WNR.0000000000002162

Somaya Saad, Ahmed F Abouelnaga, Marwa Abass, Walaa Obydah, Nedaa A Kiwan, Omar Abd-Alhakem Ammar, Osama A Abulseoud, Abdelaziz M Hussein

{"title":"Effect of deep brain stimulation for lateral hypothalamic area on memory decline and hippocampal neurofilaments expression dysfunctions in aged rats.","authors":"Somaya Saad, Ahmed F Abouelnaga, Marwa Abass, Walaa Obydah, Nedaa A Kiwan, Omar Abd-Alhakem Ammar, Osama A Abulseoud, Abdelaziz M Hussein","doi":"10.1097/WNR.0000000000002162","DOIUrl":"10.1097/WNR.0000000000002162","url":null,"abstract":"Objective: Studying the effect of deep brain stimulation (DBS) in the lateral hypothalamic area (LHA) in young and aging rats regarding memory changes, hippocampal neuronal dystrophy, and neurofilament expression.Methods: Thirty-six male Sprague-Dawley rats were divided into two main groups: adult young ( n = 18, 8 weeks old) and aged ( n = 18, 24 months old). Each main group was further subdivided into three equal subgroups ( n = 6) including control, sham, and DBS. DBS of LHA was conducted using high-frequency electric currents (130 Hz) for 1.5 h with 5-min breaks every 30 min for five consecutive days. Assessment of working memory was done using passive avoidance test (PAT). Then, the brain was dissected and hippocampal neuronal dystrophic damage was assessed as well as immunohistochemical examination of neurofilaments (NF68, NF200) expression.Results: Aging rats had progressive hippocampal neuronal degeneration and downregulation of heavy and light chain neurofilaments, that was associated with progressive decline in working memory. Nevertheless, activation of DBS in the LHA enhanced memory function as it increased latency to entry in PAT ( P < 0.001) compared to old normal and sham groups. Dystrophic damage score significantly decreased with DBS ( P < 0.001) in the hippocampal CA1, CA3, and dentate gyrus regions. Moreover, DBS upregulated hippocampal NF68, NF200 expression ( P < 0.001) in both young and old rats. We also found a significant positive correlation between working memory and NFs expression and a negative correlation between dystrophic damage score and NFs expression.Conclusions: DBS in the LHA may have a neuroprotective effect in aging rats as it enhanced the working memory and decreased hippocampal neuronal dystrophy. This protective effect may be caused by the upregulation of neurofilaments.","PeriodicalId":19213,"journal":{"name":"Neuroreport","volume":" ","pages":"402-411"},"PeriodicalIF":1.6,"publicationDate":"2025-05-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143973439","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Astrocytic mitochondria modulate poststroke splenic immune responses, promote interleukin 10 production, and mediate neuroprotection after intracerebral hemorrhage. 星形细胞线粒体调节脑卒中后脾免疫反应，促进白细胞介素10的产生，并介导脑出血后的神经保护。

IF 1.6 4区医学

Neuroreport Pub Date : 2025-05-13 DOI: 10.1097/WNR.0000000000002175

Ryosuke Tashiro, Yuki Kitamura, Jesus Bautista-Garrido, Guanghua Sun, Gab Seok Kim, Jaroslaw Aronowski, Joo Eun Jung

{"title":"Astrocytic mitochondria modulate poststroke splenic immune responses, promote interleukin 10 production, and mediate neuroprotection after intracerebral hemorrhage.","authors":"Ryosuke Tashiro, Yuki Kitamura, Jesus Bautista-Garrido, Guanghua Sun, Gab Seok Kim, Jaroslaw Aronowski, Joo Eun Jung","doi":"10.1097/WNR.0000000000002175","DOIUrl":"https://doi.org/10.1097/WNR.0000000000002175","url":null,"abstract":"We recently demonstrated that systemically transplanted astrocytic mitochondria enter the intracerebral hemorrhage (ICH)-affected brain, where they protect the neurons by mitigating oxidative damage via upregulation of the manganese superoxide dismutase (Mn-SOD), ultimately contributing to functional recovery after ICH in mice. Although our previous study clearly demonstrated the beneficial effects of mitochondria within the brain, the effect of transferred mitochondria on the peripheral system was not yet studied. Thus, here, we studied the impact of astrocytic mitochondria transfer on post-ICH recovery and modulation of systemic immune responses. We used the autologous blood injection model for the mouse ICH surgery. Mice subjected to ICH received astrocytic mitochondria intravenously at 1 h, 7, and 14 days post-ICH onset, and the splenic immune responses of these mice were analyzed at 21 days. An ICH-like injury was induced in vitro using primary cultured neurons treated with recombinant interleukin-10, and cell viability, reactive oxygen species levels, and gene expressions were analyzed. We demonstrate that systemic transplantation of astrocytic mitochondria increases the population of splenic B cells, production of interleukin-10 by B cells, and plasma interleukin-10 levels in mice after ICH. Furthermore, in the ICH-like injury in vitro, exogenous interleukin-10 (to model spleen-mediated interleukin-10 increase) upregulated Mn-SOD expression in the cultured neurons and promoted neuronal survival and neuroplasticity-related gene expressions, suggesting interleukin-10 role in cytoprotection and repair/recovery under ICH-like condition. Thus, systemic transfer of astrocytic mitochondria modulates post-ICH peripheral immune responses, which may participate in functional recovery.","PeriodicalId":19213,"journal":{"name":"Neuroreport","volume":" ","pages":""},"PeriodicalIF":1.6,"publicationDate":"2025-05-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144079166","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Temporal integration and stream segregation because of differences in base tones. 由于基音差异造成的时间整合和流隔离。

IF 1.6 4区医学

Neuroreport Pub Date : 2025-05-13 DOI: 10.1097/WNR.0000000000002173

Ken Suzutani, Tetsuya Shiga, Hiroshi Hoshino, Ayaka Arakawa, Yuhei Mori, Kazuko Kanno, Yuichi Takahashi, Tomohiro Wada, Aya Sato, Shuntaro Itagaki, Itaru Miura, Hirooki Yabe

{"title":"Temporal integration and stream segregation because of differences in base tones.","authors":"Ken Suzutani, Tetsuya Shiga, Hiroshi Hoshino, Ayaka Arakawa, Yuhei Mori, Kazuko Kanno, Yuichi Takahashi, Tomohiro Wada, Aya Sato, Shuntaro Itagaki, Itaru Miura, Hirooki Yabe","doi":"10.1097/WNR.0000000000002173","DOIUrl":"https://doi.org/10.1097/WNR.0000000000002173","url":null,"abstract":"Humans live surrounded by many sounds and have the ability to filter out various background sounds. It involves stream segregation and temporal integration. When the reference frequency is set to 3000 Hz, stream segregation will precede temporal integration when the frequency difference is 1000 Hz or larger; however, there is no report that examines whether the threshold of frequency difference at which stream segregation occurs before temporal integration is similar to different base tones. We created 10 blocks of tone sequences in which high tones and low tones were alternated with a constant stimulus onset asynchrony of 120 ms. In the first group (group A), the frequency of base tones was fixed at 3000 Hz. In the second group (group B), the frequency of base tones was fixed at 2000 Hz. The frequency of pair tones was set at 3000, 2750, 2500, 2250, and 2000 Hz in each block, respectively presented with six blocks of alternating high tones and low tones. In group A, the mean voltage in response to omission was significantly lower than the mean voltage in response to opposite tones except in the fifth block. On the other hand, in group B, the mean voltage in response to omission was significantly lower than the mean voltage in response to opposite tones in all blocks. Our results indicated that whether a temporal window of integration or stream segregation is preferred depends on the base tone's Hz.","PeriodicalId":19213,"journal":{"name":"Neuroreport","volume":" ","pages":""},"PeriodicalIF":1.6,"publicationDate":"2025-05-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144079184","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Reticulon 4 reflects endoplasmic reticulum stress in arginine vasopressin neurons. 网状4反映精氨酸加压素神经元内质网应激。

IF 1.6 4区医学

Neuroreport Pub Date : 2025-05-12 DOI: 10.1097/WNR.0000000000002174

Yohei Kawaguchi, Yuichi Hodai, Satoshi Naito, Yuichi Kondo, Tetsuro Tsumura, Takashi Miyata, Tomoko Kobayashi, Mariko Sugiyama, Takeshi Onoue, Shintaro Iwama, Hidetaka Suga, Ryoichi Banno, Hiroshi Arima, Daisuke Hagiwara

{"title":"Reticulon 4 reflects endoplasmic reticulum stress in arginine vasopressin neurons.","authors":"Yohei Kawaguchi, Yuichi Hodai, Satoshi Naito, Yuichi Kondo, Tetsuro Tsumura, Takashi Miyata, Tomoko Kobayashi, Mariko Sugiyama, Takeshi Onoue, Shintaro Iwama, Hidetaka Suga, Ryoichi Banno, Hiroshi Arima, Daisuke Hagiwara","doi":"10.1097/WNR.0000000000002174","DOIUrl":"https://doi.org/10.1097/WNR.0000000000002174","url":null,"abstract":"Arginine vasopressin (AVP) is synthesized in the magnocellular supraoptic nucleus and paraventricular nuclei of the hypothalamus, where AVP neurons function under a consistently high demand for AVP production. AVP neurons are subject to endoplasmic reticulum (ER) stress even under basal conditions, and this ER stress is further exacerbated when AVP production increases due to dehydration. Reticulon (RTN) is essential for ER formation and stabilization and plays a critical role in membrane morphogenesis within the ER. This study aimed to investigate the expression of RTN family members in hypothalamic AVP neurons. Fluorescence immunohistochemistry and in-situ hybridization were employed to examine the expression of RTN family members in hypothalamic AVP neurons of adult male mice. Water deprivation and treatment with a chemical chaperone 4-phenylbutyric acid were used to increase and decrease the ER stress of AVP neurons, respectively. Among the RTN family members, only RTN4 was found to be expressed in hypothalamic AVP neurons. RTN4 was colocalized with ER organelle markers, including immunoglobulin heavy chain binding protein and calnexin. Furthermore, RTN4 expression increased during ER stress induced by water deprivation. On the other hand, increased RTN4 expression by water deprivation was attenuated by 4-phenylbutyric acid treatment. Our results suggest that RTN4 expression in AVP neurons is closely associated with ER stress caused by increased protein production in neuroendocrine cells.","PeriodicalId":19213,"journal":{"name":"Neuroreport","volume":" ","pages":""},"PeriodicalIF":1.6,"publicationDate":"2025-05-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144079181","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Tetrandrine mediates autophagy via sirtuin 3/adenosine 5-monophosphate-activated protein kinase/mammalian target of rapamycin signal pathway to attenuate early brain injury after subarachnoid hemorrhage. 粉防己碱通过sirtuin 3/腺苷5-单磷酸活化蛋白激酶/雷帕霉素信号通路的哺乳动物靶点介导自噬，减轻蛛网膜下腔出血后早期脑损伤。

IF 1.6 4区医学

Neuroreport Pub Date : 2025-05-12 DOI: 10.1097/WNR.0000000000002171

Wenliang Wang, Yang Li, Yuan Li, Yan-Meng Zhao, Jia-Bei Ye, Tao Qian

{"title":"Tetrandrine mediates autophagy via sirtuin 3/adenosine 5-monophosphate-activated protein kinase/mammalian target of rapamycin signal pathway to attenuate early brain injury after subarachnoid hemorrhage.","authors":"Wenliang Wang, Yang Li, Yuan Li, Yan-Meng Zhao, Jia-Bei Ye, Tao Qian","doi":"10.1097/WNR.0000000000002171","DOIUrl":"https://doi.org/10.1097/WNR.0000000000002171","url":null,"abstract":"Early brain injury (EBI) is the main cause of poor outcomes in patients with subarachnoid hemorrhage (SAH). Tetrandrine (Tet) is the root of Stephania tetrandra S Moore extract that has been shown to promote neuronal survival and regulate a variety of signaling pathways; however, the mechanism through which it exerts neuroprotective effects in patients with SAH is unknown. This investigation was to examine Tet's effect on EBI in SAH rats. We divided the rats into four groups. The effects of Tet treatment on the pathological changes of neurons in rat brains were evaluated, as well as autophagy-related and signaling pathway proteins. We found that Tet had a neuroprotective effect on EBI after SAH, as evidenced by the fact that Tet ameliorated SAH-mediated neurologic impairment and neuronal morphological damage and reduced brain water content, neuronal apoptosis rate, and neuronal cell loss. Tet decreased the LC3II/LC3I ratio, elevated P62 protein expression, and inhibited autophagosome production after SAH. Tet may have increased sirtuin 3 (SIRT3) expression, decreased adenosine 5-monophosphate-activated protein kinase (AMPK) phosphorylation, and increased phosphor-mammalian target of rapamycin (mTOR) levels, all of which may have occurred particularly via SIRT3/AMPK/mTOR signaling pathway activation; However, this trend can be reversed by 3-(1H-1,2,3-triazol-4-yl) pyridine (SIRT3 inhibitors). Tet exerts neuroprotective effects by inhibiting autophagy, this may be associated with SIRT3's inhibitory effect on the AMPK/mTOR signaling pathway. This inhibition could function as a potential mechanism for the neuroprotective effects observed in patients suffering from SAH.","PeriodicalId":19213,"journal":{"name":"Neuroreport","volume":" ","pages":""},"PeriodicalIF":1.6,"publicationDate":"2025-05-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144079192","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Alterations in static and dynamic functional network connectivity in chronic low back pain: a resting-state network functional connectivity and machine learning study. 慢性腰痛的静态和动态功能网络连接的改变：静息状态网络功能连接和机器学习研究。

IF 1.6 4区医学

Neuroreport Pub Date : 2025-05-07 Epub Date: 2025-04-09 DOI: 10.1097/WNR.0000000000002158

Hao Liu, Xin Wan

{"title":"Alterations in static and dynamic functional network connectivity in chronic low back pain: a resting-state network functional connectivity and machine learning study.","authors":"Hao Liu, Xin Wan","doi":"10.1097/WNR.0000000000002158","DOIUrl":"https://doi.org/10.1097/WNR.0000000000002158","url":null,"abstract":"Low back pain (LBP) is a prevalent pain condition whose persistence can lead to changes in the brain regions responsible for sensory, cognitive, attentional, and emotional processing. Previous neuroimaging studies have identified various structural and functional abnormalities in patients with LBP; however, how the static and dynamic large-scale functional network connectivity (FNC) of the brain is affected in these patients remains unclear. Forty-one patients with chronic low back pain (cLBP) and 42 healthy controls underwent resting-state functional MRI scanning. The independent component analysis method was employed to extract the resting-state networks. Subsequently, we calculate and compare between groups for static intra- and inter-network functional connectivity. In addition, we investigated the differences between dynamic functional network connectivity and dynamic temporal metrics between cLBP patients and healthy controls. Finally, we tried to distinguish cLBP patients from healthy controls by support vector machine method. The results showed that significant reductions in functional connectivity within the network were found within the DMN,DAN, and ECN in cLBP patients. Significant between-group differences were also found in static FNC and in each state of dynamic FNC. In addition, in terms of dynamic temporal metrics, fraction time and mean dwell time were significantly altered in cLBP patients. In conclusion, our study suggests the existence of static and dynamic large-scale brain network alterations in patients with cLBP. The findings provide insights into the neural mechanisms underlying various brain function abnormalities and altered pain experiences in patients with cLBP.","PeriodicalId":19213,"journal":{"name":"Neuroreport","volume":"36 7","pages":"364-377"},"PeriodicalIF":1.6,"publicationDate":"2025-05-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144014809","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Dancers' resistance to vertigo cannot be explained by a generalized suppression of vestibular signaling. 舞者对眩晕的抵抗不能用前庭信号的普遍抑制来解释。

IF 1.6 4区医学

Neuroreport Pub Date : 2025-05-07 Epub Date: 2025-04-09 DOI: 10.1097/WNR.0000000000002160

Karina Moïn-Darbari, Daniel Paromov, Benoit-Antoine Bacon, Maxime Maheu, François Champoux

{"title":"Dancers' resistance to vertigo cannot be explained by a generalized suppression of vestibular signaling.","authors":"Karina Moïn-Darbari, Daniel Paromov, Benoit-Antoine Bacon, Maxime Maheu, François Champoux","doi":"10.1097/WNR.0000000000002160","DOIUrl":"https://doi.org/10.1097/WNR.0000000000002160","url":null,"abstract":"From a vestibular perspective, it is remarkable that dancers are often performing challenging tasks such as pirouettes, and yet manage to do so without falling. Some have suggested that dancers' resistance to vertigo may be explained by a generalized suppression of vestibular signaling. Here, we aimed to test this hypothesis by examining the impact of galvanic vestibular stimulation (GVS) on postural control in dancers. A total of 38 participants were recruited for this study and were divided into two groups: 19 dancers and 19 healthy controls. Postural control was assessed at baseline and during GVS. As expected, dancers exhibited better postural control, as assessed by a decrease in sway area, when compared to the control group in the baseline condition. However, contrary to expectations, dancers did not differ from controls during GVS. This confirms that dancers' resistance to vertigo cannot be explained by a generalized suppression of vestibular signaling. Rather, dancers may have developed a more accurate body representation due to top-down modulation of subcortical neuronal networks and may only be able to modify vestibular input during active movement.","PeriodicalId":19213,"journal":{"name":"Neuroreport","volume":"36 7","pages":"378-381"},"PeriodicalIF":1.6,"publicationDate":"2025-05-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144006202","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Correlation between basal ganglia nuclei and cortical gray matter volume changes in different motor subtypes of Parkinson's disease. 帕金森病不同运动亚型基底神经节核与皮质灰质体积变化的相关性

IF 1.6 4区医学

Neuroreport Pub Date : 2025-05-07 Epub Date: 2025-04-09 DOI: 10.1097/WNR.0000000000002155

Jun Lu, Xinyue Huangfu, Yumei Zhang, Yan Nan, Minhai Wang, Weilan Zhang, Chengwei Wang

{"title":"Correlation between basal ganglia nuclei and cortical gray matter volume changes in different motor subtypes of Parkinson's disease.","authors":"Jun Lu, Xinyue Huangfu, Yumei Zhang, Yan Nan, Minhai Wang, Weilan Zhang, Chengwei Wang","doi":"10.1097/WNR.0000000000002155","DOIUrl":"https://doi.org/10.1097/WNR.0000000000002155","url":null,"abstract":"Explore the correlation between basal ganglia nuclei and cortical gray matter volume changes in tremor-dominant and postural instability-gait difficulty (PIGD) Parkinson's disease subtypes for Parkinson's disease diagnosis and individualized treatment. High-resolution 3D-T1WI MRI data from 35 tremor-dominant and 30 PIGD patientsand 35 healthy controls were analyzed. Voxel-based morphometry identified gray matter volume differences. Automated basal ganglia segmentation quantified subcortical volumes, followed by multivariate analysis of covariance and Spearman correlation analyses. Compared with healthy control, patients with PIGD exhibited severe gray matter loss (P < 0.0001), while tremor-dominant showed nonsignificant reductions. Subcortically, different basal ganglia volumes were atrophied in the tremor-dominant and PIGD groups compared with the healthy control (P < 0.05). PIGD demonstrated greater left putamen atrophy than tremor-dominant (P < 0.05). Spearman correlation analysis revealed that the volume of the right globus pallidus was positively correlated with that of the left medial and lateral cingulate gyrus in patients with tremor-dominant (r = 0.35, P = 0.04); and between the left globus pallidus volume and the right superior temporal gyrus volume in patients with PIGD (r = 0.47, P = 0.01). Compared with the tremor-dominant subtype, the PIGD subtype exhibits more severe GM atrophy, with different basal ganglia volume changes across subtypes. These altered anatomical features and the correlation between degeneration of the basal ganglia region and cortical gray matter changes may provide insights into the differential functional changes in patients with different motor subtypes and help to elucidate the underlying pathologic mechanisms.","PeriodicalId":19213,"journal":{"name":"Neuroreport","volume":"36 7","pages":"342-349"},"PeriodicalIF":1.6,"publicationDate":"2025-05-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143972826","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Long noncoding RNA MALAT1 promotes angiogenesis through the caveolin-1/VEGF pathway after cerebral ischemic injury. 长链非编码RNA MALAT1在脑缺血损伤后通过caveolin-1/VEGF通路促进血管生成。

IF 1.6 4区医学

Neuroreport Pub Date : 2025-05-07 Epub Date: 2025-04-09 DOI: 10.1097/WNR.0000000000002157

Yao Lin, Qiongyi Pang, Yuanxi Shi, Xiang Chen, Fengxia Tu

{"title":"Long noncoding RNA MALAT1 promotes angiogenesis through the caveolin-1/VEGF pathway after cerebral ischemic injury.","authors":"Yao Lin, Qiongyi Pang, Yuanxi Shi, Xiang Chen, Fengxia Tu","doi":"10.1097/WNR.0000000000002157","DOIUrl":"https://doi.org/10.1097/WNR.0000000000002157","url":null,"abstract":"The long noncoding RNA metastasis-associated lung adenocarcinoma transcript 1 (MALAT1) might protect against cerebral ischemic injury. This study explored MALAT1's function in ischemic stroke and whether it acts through the caveolin-1/vascular endothelial growth factor (VEGF) pathway. A mouse model of middle cerebral artery occlusion/reperfusion (MCAO/R) and a human brain microvascular endothelial cell (HBMEC) model of oxygen-glucose deprivation/reoxygenation (OGD/R) were established. Lentiviral vectors for MALAT1 knockdown, caveolin-1 knockdown, and MALAT1 overexpression were used for gene regulation studies. Neurological deficits, endothelial cell proliferation, cell apoptosis, cell viability, in vitro angiogenesis, cell migration, and the expression of related gene and protein were evaluated using the Zea Longa five-point scale, VEGF receptor 2/CD34 double immunofluorescence, TdT-mediated dUTP nick end labeling staining, cell counting kit-8 assay, tube formation assay, transwell assay, quantitative real time PCR, and western blot. In mouse MCAO/R model and HBMEC OGD/R model, the expression levels of MALAT1, caveolin-1, and VEGF were significantly upregulated compared to the control group. In vivo, downregulation of MALAT1 expression exacerbated cerebral ischemic injury as manifested by severe neurological deficits, larger infarct volume, increased apoptosis, decreased numbers of VEGF receptor 2+/CD34+ endothelial progenitor cells, increased cell apoptosis, and the downregulation of caveolin-1 and VEGF. Conversely, overexpression of MALAT1 partially reversed the inhibition of cell migration and tubule formation by caveolin-1 gene downregulation, and restored in the expression of caveolin-1 and VEGF. MALAT1 promotes angiogenesis after cerebral ischemic injury, likely in part via the caveolin-1/VEGF pathway. Thus, MALAT1 may serve as a potential therapeutic target for ischemic stroke.","PeriodicalId":19213,"journal":{"name":"Neuroreport","volume":"36 7","pages":"350-363"},"PeriodicalIF":1.6,"publicationDate":"2025-05-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144021596","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Code conflict in an event file task is reflected by aperiodic neural activity. 事件文件任务中的代码冲突通过非周期神经活动反映出来。

IF 1.6 4区医学

Neuroreport Pub Date : 2025-05-07 Epub Date: 2025-04-09 DOI: 10.1097/WNR.0000000000002156

Jimin Yan, Lorenza Colzato, Bernhard Hommel

{"title":"Code conflict in an event file task is reflected by aperiodic neural activity.","authors":"Jimin Yan, Lorenza Colzato, Bernhard Hommel","doi":"10.1097/WNR.0000000000002156","DOIUrl":"https://doi.org/10.1097/WNR.0000000000002156","url":null,"abstract":"We investigated the relationship between aperiodic electroencephalography (EEG) activity and code conflict, hypothesizing that the former might serve as an indicator of the latter. We analyzed EEG and behavioral outcomes of a sample performing the event file task, which assesses code conflict in co-occurring or temporally overlapping stimulus and response features. To quantify aperiodic activity, we employed the fitting oscillations & one-over-f algorithm. The behavioral results revealed a typical partial-repetition cost effect, indicating that performance is impaired if the stimulus repeats while the response alternates, or vice versa. This suggests that the previously combined shape and response were stored in an event file and retrieved when any one of these components was repeated. Notably, this effect was also evident in the aperiodic exponent, which was lower for partial repetitions than for full repetitions or alternations, implying increased cortical noise, a higher excitatory E/I ratio, and noisier decision-making processes. The scalp distribution of this effect aligns with its sensorimotor characteristics. Thus, we interpret these findings as promising preliminary evidence that the aperiodic exponent may serve as a valuable neural marker of code conflict.","PeriodicalId":19213,"journal":{"name":"Neuroreport","volume":"36 7","pages":"337-341"},"PeriodicalIF":1.6,"publicationDate":"2025-05-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144021594","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0