IF 1.6 4区医学

Neuroreport Pub Date : 2025-06-04 Epub Date: 2025-05-14 DOI: 10.1097/WNR.0000000000002167

Zhifeng Wang, Yujiang Xi, Junfeng Lan, Jiao Yang, Ting Shi, Shuangfeng Xu, Liwei Xing, Pan Pan, Jian Wang

{"title":"Effects of electroacupuncture on neural function and the expression of inflammation-related proteins NLRP3/caspase-1 in rats with ischemic stroke.","authors":"Zhifeng Wang, Yujiang Xi, Junfeng Lan, Jiao Yang, Ting Shi, Shuangfeng Xu, Liwei Xing, Pan Pan, Jian Wang","doi":"10.1097/WNR.0000000000002167","DOIUrl":"10.1097/WNR.0000000000002167","url":null,"abstract":"This study aimed to investigate the neuroprotective effects of electroacupuncture (EA) at the Baihui and Dazhui acupoints in a rat model of ischemia-reperfusion injury. Ninety-six male Sprague-Dawley rats were randomly assigned to four groups ( n = 24 per group): Sham, middle cerebral artery occlusion (MCAO), MCAO+EA, and MCAO+MCC950. The MCAO model was induced using filament embolization. Neurological function was assessed using Zea Longa scores on days 1 and 7 posttreatment, while cerebral infarction volume was measured using 2,3,5-triphenyltetrazolium chloride staining. Gene expression levels of NLRP3 and GSDMD were quantified by RT-qPCR, and protein expressions of NLRP3, GSDMD, caspase-1, IL-1β, and IL-18 were evaluated via Western blotting, immunohistochemistry, immunofluorescence staining, and ELISA. On day 1, compared with the MCAO group, the MCAO+EA and MCAO+MCC950 groups exhibited significantly reduced mRNA and protein expressions of NLRP3 and GSDMD ( P < 0.05), as well as decreased levels of caspase-1, IL-1β, and IL-18 ( P < 0.05). However, no significant differences were observed in neurological deficit scores or cerebral infarction volume. By day 7, both the MCAO+EA and MCAO+MCC950 groups showed significant improvements in neurological function ( P < 0.05), reductions in cerebral infarction volume ( P < 0.05), and further decreases in the expression of NLRP3, GSDMD, caspase-1, IL-1β, and IL-18 ( P < 0.05). EA at the Baihui and Dazhui acupoints alleviates neurological deficits in ischemic stroke rats by inhibiting the NLRP3/caspase-1 inflammatory pathway and reducing the expression of apoptosis-related proteins such as NLRP3, GSDMD, caspase-1, IL-1β, and IL-18.","PeriodicalId":19213,"journal":{"name":"Neuroreport","volume":" ","pages":"456-466"},"PeriodicalIF":1.6,"publicationDate":"2025-06-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12080364/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144079170","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Electroacupuncture promotes functional recovery after spinal cord injury in rats by regulating P2X4R/p38 MAPK signaling pathway and suppressing inflammatory responses. 电针通过调节P2X4R/p38 MAPK信号通路，抑制炎症反应，促进大鼠脊髓损伤后功能恢复。

IF 1.6 4区医学

Neuroreport Pub Date : 2025-06-04 Epub Date: 2025-05-14 DOI: 10.1097/WNR.0000000000002163

Xiang Wang, Yimin Gao, Jianzhong Huo

{"title":"Electroacupuncture promotes functional recovery after spinal cord injury in rats by regulating P2X4R/p38 MAPK signaling pathway and suppressing inflammatory responses.","authors":"Xiang Wang, Yimin Gao, Jianzhong Huo","doi":"10.1097/WNR.0000000000002163","DOIUrl":"10.1097/WNR.0000000000002163","url":null,"abstract":"This study aimed to investigate whether electroacupuncture can modulate the purinergic P2X4 receptor (P2X4R)/p38 mitogen-activated protein kinase (MAPK) pathway, thereby reducing inflammatory responses and facilitating functional recovery in a rat model of spinal cord injury (SCI). The SCI model was developed in female rats. The electroacupuncture intervention began on the seventh day after modeling, mainly Jiaji, Dazhui, and Mingmen. Sensory function was evaluated via the mechanical withdrawal threshold (MWT) and thermal withdrawal latency (TWL), while motor function was measured using the Basso, Beattie, and Bresnahan (BBB) scoring system and footprint analysis. To analyze the protein expression related to the P2X4R/p38 MAPK signaling pathways, methods such as immunohistochemistry, immunofluorescence analysis, quantitative real-time PCR, and western blotting were utilized. To evaluate the levels of inflammatory cytokines, ELISAs were utilized. Additionally, after hematoxylin and eosin staining, histological alterations in spinal cord tissue were investigated. The results showed that MWT, TWL, and BBB scores were decreased, while P2X4R, phosphorylated-p38 MAPK, and phosphorylated nuclear factor κB p65 expression levels were increased, tumor necrosis factor-α, interleukin (IL)-1β, and IL-6 levels were elevated, and histopathological damage was more pronounced after SCI. However, electroacupuncture treatment effectively reversed these pathological changes. We demonstrate that electroacupuncture can alleviate SCI in rats by inhibiting the activation of the P2X4R/p38 MAPK pathway and reducing inflammatory response.","PeriodicalId":19213,"journal":{"name":"Neuroreport","volume":" ","pages":"443-455"},"PeriodicalIF":1.6,"publicationDate":"2025-06-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143983492","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Inhibition of autophagy in the amygdala ameliorates anxiety-like behaviors induced by morphine-protracted withdrawal in male mice. 抑制杏仁核自噬可改善雄性小鼠吗啡持久戒断诱导的焦虑样行为。

IF 1.6 4区医学

Neuroreport Pub Date : 2025-06-04 Epub Date: 2025-04-23 DOI: 10.1097/WNR.0000000000002166

Shuang Han, Chenchen Zhu, Dengjun Min, Zicheng Li

{"title":"Inhibition of autophagy in the amygdala ameliorates anxiety-like behaviors induced by morphine-protracted withdrawal in male mice.","authors":"Shuang Han, Chenchen Zhu, Dengjun Min, Zicheng Li","doi":"10.1097/WNR.0000000000002166","DOIUrl":"10.1097/WNR.0000000000002166","url":null,"abstract":"Objective: Morphine withdrawal triggers a range of negative affective states, wherein anxiety is typically common, significantly contributing to the morphine relapse. To date, the exact mechanism underlying morphine withdrawal-induced anxiety has remained unclear. Previous studies have proposed that autophagy is involved in the pathogenesis of morphine addiction and anxiety; however, the possible relationship between autophagy and morphine withdrawal-induced anxiety has not been explored before. In this study, we aimed to reveal the potential role of autophagy in anxiety-like behaviors elicited by protracted morphine withdrawal, and which brain region is involved.Methods: We established the model mice of anxiety by chronic intermittent escalating-dose morphine administration for 7 days and then withdrawing for 4 days. Anxious behaviors were detected using the Open field test and the Elevated plus maze test. Western blot was performed to measure the change of autophagy-associated proteins (ATG5, Beclin-1, LC3) in different brain regions.Results: Our results showed that intraperitoneal injection of an autophagy inhibitor 3-Methyladenine attenuated protracted morphine withdrawal-induced anxiety-like behaviors in male mice. Moreover, protracted morphine withdrawal predominantly promoted autophagy in the amygdala, rather than other related brain regions, suggesting the crucial involvement of amygdala in autophagy-mediated anxiety after morphine withdrawal. We further validated that 3-Methyladenine can effectively reduce autophagy-associated protein levels in the relevant brain region.Conclusion: These findings indicated that protracted morphine withdrawal-elicited autophagy in the amygdala contributes to the anxiety-like behaviors and may have implications for the future treatment of this disorder.","PeriodicalId":19213,"journal":{"name":"Neuroreport","volume":" ","pages":"487-496"},"PeriodicalIF":1.6,"publicationDate":"2025-06-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12084013/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144007081","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Network centrality alterations in patients with moyamoya disease after combined revascularization surgery: a resting-state fMRI study. 烟雾病患者联合血运重建术后网络中心性的改变：静息状态fMRI研究

IF 1.6 4区医学

Neuroreport Pub Date : 2025-06-04 Epub Date: 2025-05-14 DOI: 10.1097/WNR.0000000000002154

Yuanyuan Wang, Jian Li, Feng Lin, Junwei Gao, Zihe Xu, Jie Liu, Xianjun Zeng

{"title":"Network centrality alterations in patients with moyamoya disease after combined revascularization surgery: a resting-state fMRI study.","authors":"Yuanyuan Wang, Jian Li, Feng Lin, Junwei Gao, Zihe Xu, Jie Liu, Xianjun Zeng","doi":"10.1097/WNR.0000000000002154","DOIUrl":"10.1097/WNR.0000000000002154","url":null,"abstract":"Objective: This study aimed to explore alterations in brain network characteristics among patients with moyamoya disease (MMD) before and after combined revascularization surgery (CRS).Methods: This investigation enrolled 20 MMD patients alongside 20 age- and sex-matched healthy controls (HCs). All participants underwent MRI scans. Additionally, MMD patients were subjected to comprehensive clinical assessments. Degree centrality (DC) analysis was utilized to assess the connectivity features of the entire brain network. The study also examined correlations between DC values in MMD patients before- (pre-CRS) and after-CRS (post-CRS) and various clinical indicators.Results: Compared with HCs, pre-CRS MMD patients showed abnormal DC values in multiple brain regions, mainly including the cerebellum, frontal lobe, temporal lobe, and cingulate gyrus. One year after CRS treatment, the DC values of the bilateral cerebellum posterior lobe showed a reverse increase. In addition, the DC value of the right cerebellum posterior lobe in pre-CRS MMD patients was positively correlated with the Montreal cognitive assessment scores.Conclusions: CRS treatment can effectively improve the functional network damage of the bilateral cerebellum posterior lobes caused by MMD, and it is expected to provide a new neuroimaging marker for the evaluation of CRS treatment efficacy.","PeriodicalId":19213,"journal":{"name":"Neuroreport","volume":" ","pages":"435-442"},"PeriodicalIF":1.6,"publicationDate":"2025-06-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144028694","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Mendelian randomization combined with single-cell sequencing data analysis of chemokines and chemokine receptors and key genes and molecular mechanisms associated with epilepsy. 孟德尔随机化结合单细胞测序数据分析趋化因子和趋化因子受体与癫痫相关的关键基因和分子机制。

IF 1.6 4区医学

Neuroreport Pub Date : 2025-06-04 Epub Date: 2025-04-29 DOI: 10.1097/WNR.0000000000002168

Lin-Ming Zhang, Tao Zeng, Bing-Ran Zhang, Qiu-Juan Zhang, Shu-Ji Gao, Yan-Lin Zhu, Ming-Wei Liu

{"title":"Mendelian randomization combined with single-cell sequencing data analysis of chemokines and chemokine receptors and key genes and molecular mechanisms associated with epilepsy.","authors":"Lin-Ming Zhang, Tao Zeng, Bing-Ran Zhang, Qiu-Juan Zhang, Shu-Ji Gao, Yan-Lin Zhu, Ming-Wei Liu","doi":"10.1097/WNR.0000000000002168","DOIUrl":"10.1097/WNR.0000000000002168","url":null,"abstract":"Objective: To explore the functions and potential regulatory mechanisms of chemokine and chemokine receptor (CCR)-related genes in epilepsy.Methods: CCRs were identified as candidate genes and their causal relationship with epilepsy was rigorously evaluated via Mendelian randomization analysis. Subsequently, single-cell RNA sequencing (scRNA-seq) data were analyzed to identify and classify cell clusters into distinct types based on cellular annotation. Differential expression analysis was conducted to pinpoint key genes by overlapping the candidate gene set with differentially expressed genes (DEGs). Furthermore, potential therapeutic drugs for epilepsy were predicted, offering novel avenues for disease management and treatment.Results: In total, 6395 DEGs were identified across the six cell clusters. After their intersection, CCRL2, XCL2, CXCR5, CXCL1, and CX3CR1 were pinpointed as key genes. Microglia, T cells, B cells, and macrophages have been emerged as critical cells. Furthermore, CXCL1 was regulated by hsa-miR-570-3p and hsa-miR-532-5p. Notably, CXCR5, CXCL1, and CX3CR1 were associated with 27 drug compounds. This comprehensive study leveraged scRNA-seq and transcriptomic data to elucidate the roles of CCR-related genes in epilepsy. Notably, CCRL2, XCL2, CXCR5, CXCL1,and CX3CR1 were identified as key genes implicated in epilepsy, whereas microglia, T cells, B cells, and macrophages were recognized as critical contributors to the development of epilepsy.Conclusions: Regulating the expression of CCRL2, XCL2, CXCR5, CXCL1, and CX3CR1, along with the activity of these immune cells may offer therapeutic potential for the alleviation of epilepsy.","PeriodicalId":19213,"journal":{"name":"Neuroreport","volume":" ","pages":"467-486"},"PeriodicalIF":1.6,"publicationDate":"2025-06-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12080367/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144064290","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

AAV-mediated transgene delivery targeting spiral ganglion nonsensory cells. aav介导的螺旋神经节非感觉细胞的转基因传递。

IF 1.6 4区医学

Neuroreport Pub Date : 2025-06-04 Epub Date: 2025-05-14 DOI: 10.1097/WNR.0000000000002172

Joshua S Lin, Nhi V Nguyen, Seiji B Shibata

{"title":"AAV-mediated transgene delivery targeting spiral ganglion nonsensory cells.","authors":"Joshua S Lin, Nhi V Nguyen, Seiji B Shibata","doi":"10.1097/WNR.0000000000002172","DOIUrl":"10.1097/WNR.0000000000002172","url":null,"abstract":"In-situ neuronal reprogramming in the cochlea through gene therapy offers an avenue to restore hearing loss caused by neuronal damage. One possible source of neuronal conversion is the nonspiral ganglion cells (NSGCs), which include satellite cells, Schwann cells, and otic mesenchyme cells. A major obstacle for this approach is the vector-mediated transgene delivery toward NSGCs. Herein, we sought to assess the transduction profile of adeno-associated virus (AAV) serotypes with peripheral glial cell tropism in the murine inner ear. AAV-1, AAV-DJ, and AAV-PHP.eB with a cytomegalovirus promoter-driven enhanced green flourescent protein (eGFP) reporter were injected into CBA/CaJ neonatal mice via the posterior semicircular canal. One week postinjection, the cochlear tissue was collected for immunohistochemistry in whole-mount and mid-modiolar sections to assess the colocalization of eGFP within the NSGCs in the osseous spiral lamina and Rosenthal's canal. The contralateral ear served as an internal control. Auditory brain responses (ABRs) were recorded 30 days postinjection to assess for hearing loss. AAV-1 and AAV-DJ demonstrated 30-32% transduction efficacy of Pou3f4 immunopositive otic mesenchyme cells, whereas transduction efficacy of Sox2 or Sox10 positive Schwann cells and satellite cells was 0.8-1.82% for all serotypes. At 30 days postinjection, ABR thresholds in the injected mice were comparable to those of the noninjected control. We were able to transduce otic mesenchyme cells among SGNCs in the spiral ganglion region, whereas transduction of Schwann cells and satellite cells continues to pose challenges with AAV-1, AAV-DJ, and AAV-PHP.eB serotypes.","PeriodicalId":19213,"journal":{"name":"Neuroreport","volume":" ","pages":"497-503"},"PeriodicalIF":1.6,"publicationDate":"2025-06-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12080363/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144079165","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Sex differences in seizure presentation in a Dravet syndrome mouse model. Dravet综合征小鼠模型中癫痫表现的性别差异。

IF 1.6 4区医学

Neuroreport Pub Date : 2025-05-14 Epub Date: 2025-04-01 DOI: 10.1097/WNR.0000000000002159

Sheryl Anne D Vermudez, Rui Lin, Gabrielle E McGinty, Yongho Choe, Amanda Liebhardt, Benjamin Hui, Ella Lubbers, Sameer C Dhamne, Mustafa Q Hameed, Alexander Rotenberg

{"title":"Sex differences in seizure presentation in a Dravet syndrome mouse model.","authors":"Sheryl Anne D Vermudez, Rui Lin, Gabrielle E McGinty, Yongho Choe, Amanda Liebhardt, Benjamin Hui, Ella Lubbers, Sameer C Dhamne, Mustafa Q Hameed, Alexander Rotenberg","doi":"10.1097/WNR.0000000000002159","DOIUrl":"10.1097/WNR.0000000000002159","url":null,"abstract":"Objectives: Dravet syndrome is an epileptic encephalopathy mostly because of haploinsufficiency of the SCN1A voltage-gated sodium channel subunit. Disease presentation (i.e. severe seizures and early life mortality) is faithfully modeled in mice haploinsufficient in Scn1a ( Scn1a+/ - ). However, the characterization of sex differences in mortality and seizure morbidity is limited. Given the reliance of mouse models for studying disease pathophysiology and for the development of novel treatments, we tested whether disease presentation differed in juvenile and adult female and male Scn1a+/ - mice.Methods: Mortality and seizure morbidity were quantified in juvenile and adult female and male Scn1a+/ - animals on an F1 hybrid C57 × 129S6 background.Results: Mortality was significantly higher in female Scn1a+/ - mice compared with males regardless of age, and juvenile female Scn1a+/ - mice had a significantly lower febrile seizure threshold than age-matched Scn1a+/ - males. Conversely, long-term video EEG recordings revealed that adult male Scn1a+/ - mice had significantly more frequent and longer spontaneous seizures than adult females. Adult female Scn1a+/- mice, however, were significantly more hyperactive, which may indicate sleep impairment.Conclusion: The phenotypic presentation of Scn1a+/ - mice is sex-dependent which may have translational implications for understanding basic pathophysiological mechanisms as well as therapeutic drug discovery in Dravet syndrome.","PeriodicalId":19213,"journal":{"name":"Neuroreport","volume":" ","pages":"383-388"},"PeriodicalIF":1.6,"publicationDate":"2025-05-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143983493","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Changes in topological properties of brain structural covariance networks and alertness in temporal lobe epilepsy with and without focal to bilateral tonic-clonic seizures. 颞叶癫痫伴或不伴局灶性至双侧强直-阵挛性发作时脑结构协方差网络拓扑特性和警觉性的变化。

IF 1.6 4区医学

Neuroreport Pub Date : 2025-05-14 Epub Date: 2025-05-07 DOI: 10.1097/WNR.0000000000002164

Chuanyong Qu, Zexiang Chen, Shujun Su, Cuimi Luo, Ligen Fan, Yuting Sun, Jinou Zheng

{"title":"Changes in topological properties of brain structural covariance networks and alertness in temporal lobe epilepsy with and without focal to bilateral tonic-clonic seizures.","authors":"Chuanyong Qu, Zexiang Chen, Shujun Su, Cuimi Luo, Ligen Fan, Yuting Sun, Jinou Zheng","doi":"10.1097/WNR.0000000000002164","DOIUrl":"10.1097/WNR.0000000000002164","url":null,"abstract":"This study investigated brain structural covariance network (SCN) topological changes and alertness in temporal lobe epilepsy (TLE) with and without focal to bilateral tonic-clonic seizures (FBTCS). Seventy-eight subjects, including 32 TLE patients with FBTCS (TLE-FBTCS), 46 TLE patients without FBTCS (TLE-FS), and 42 healthy controls (HCs), underwent the Attention Network Test to assess alertness and volumetric MRI scans. SCNs were constructed and analyzed using graph theory. Results showed that TLE-FS patients had lower total cerebral volume than HCs, and the lowest volume was observed in the TLE-FBTCS group. Compared to HCs and TLE-FBTCS patients, TLE-FS patients exhibited increased small-worldness, normalized clustering coefficient, global efficiency, and modularity, but decreased normalized characteristic shortest path length and assortativity. Specific brain regions, such as the hippocampus, thalamus, and superior temporal sulcus, showed changes in nodal clustering coefficients and efficiency in TLE-FS patients. Further analysis revealed decreased intrinsic/phasic alertness in TLE-FBTCS patients. Correlation analysis indicated that SCN topological properties were associated with alertness in TLE-FS patients but not in TLE-FBTCS patients. These findings suggest that TLE-FS and TLE-FBTCS patients show different changes in SCN integration and segregation, with TLE-FS alertness linked to SCN topological properties, providing insights into TLE's neuropathological mechanisms.","PeriodicalId":19213,"journal":{"name":"Neuroreport","volume":" ","pages":"421-434"},"PeriodicalIF":1.6,"publicationDate":"2025-05-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144045989","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Multimodal neuroimaging alterations and host genetic associations in patients with rhegmatogenous retinal detachment: a transcriptomic-neuroimaging study. 孔源性视网膜脱离患者的多模态神经影像学改变和宿主遗传关联：一项转录组神经影像学研究。

IF 1.6 4区医学

Neuroreport Pub Date : 2025-05-14 Epub Date: 2025-04-11 DOI: 10.1097/WNR.0000000000002161

Yu Ji, Yi-Chong Duan, Lin Zhou, Hua Chai, Hao-Yu Yuan, Zhuo-Er Dong, Li-Li Yao, Xiao-Rong Wu

{"title":"Multimodal neuroimaging alterations and host genetic associations in patients with rhegmatogenous retinal detachment: a transcriptomic-neuroimaging study.","authors":"Yu Ji, Yi-Chong Duan, Lin Zhou, Hua Chai, Hao-Yu Yuan, Zhuo-Er Dong, Li-Li Yao, Xiao-Rong Wu","doi":"10.1097/WNR.0000000000002161","DOIUrl":"10.1097/WNR.0000000000002161","url":null,"abstract":"Previous neuroimaging studies have identified functional and structural changes in the gray matter of rhegmatogenous retinal detachment (RRD) patients, yet the genetic mechanisms behind these alterations remain unclear. We employed multimodal imaging to investigate gray matter alterations in RRD patients. A transcriptome-neuroimaging spatial correlation analysis, integrating gene expression data from the Allen Human Brain Atlas, identified genes linked to functional stability changes. We followed this with gene enrichment, protein-protein interaction (PPI) network mapping, and expression profiling. RRD patients showed distinct, sustained dynamic balance within the default mode network functionally, and a significant reduction in gray matter volume in the visual network region structurally, compared with healthy controls. Transcriptome-neuroimaging correlation analysis revealed a spatial link between functional and structural changes and the expression profiles of 165 genes involved in membrane organization, neurodegeneration, phagocytosis, and calcium signaling. These genes form a highly interconnected PPI network, centered around key hub genes. Tissue- and cell-specific expression analysis highlighted a distinct gene expression pattern, especially in D1 receptor-positive cells in the caudate-putamen. Our findings indicate alterations in gray matter function and structure in RRD patients, particularly in regions involved in visual and cognitive processing. Transcriptomic neuroimaging analysis reveals that these changes are linked to the expression of multiple genes, shedding light on potential genetic mechanisms underlying RRD-associated gray matter modifications and offering new insights for treatment and prognosis.","PeriodicalId":19213,"journal":{"name":"Neuroreport","volume":" ","pages":"389-401"},"PeriodicalIF":1.6,"publicationDate":"2025-05-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143972645","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Stimulus-preceding negativity and P3 reflecting relative amounts of attention allocation between primary and secondary tasks. 刺激前负性和P3反映了主要和次要任务之间的相对注意力分配量。

IF 1.6 4区医学

Neuroreport Pub Date : 2025-05-14 Epub Date: 2025-04-11 DOI: 10.1097/WNR.0000000000002165

Yasunori Kotani, Atsushi Horai, Yoshimi Ohgami, Lucian Gheorghe

{"title":"Stimulus-preceding negativity and P3 reflecting relative amounts of attention allocation between primary and secondary tasks.","authors":"Yasunori Kotani, Atsushi Horai, Yoshimi Ohgami, Lucian Gheorghe","doi":"10.1097/WNR.0000000000002165","DOIUrl":"10.1097/WNR.0000000000002165","url":null,"abstract":"Objective: This study examined event-related potentials, particularly P3, in two attention states: a 'focused attention state', wherein attention was fully allocated to a primary task, and a 'poor attention state', wherein attention was disrupted by a secondary task, to elucidate attention allocation mechanisms during multitasking.Methods: P3 was recorded under two conditions. The focus condition (focused attention state) involved only the primary task. The split-attention condition (poor attention state) included both primary and secondary tasks. Stimulus-preceding negativity (SPN) evoked by the secondary task was also recorded in the split-attention condition.Results: P3 amplitude was significantly higher in the focus condition than in the split-attention condition. Importantly, a novel finding revealed a negative correlation between SPN amplitude for the secondary task and P3 amplitude for the primary task, suggesting a trade-off in attention allocation. Source analysis of the P3 revealed that regions associated with attention shifting and inhibitory control were active during the focus condition. In contrast, regions linked to impaired cognitive-sensory integration and increased impulsivity were prominent during the split-attention condition.Conclusion: This study highlights a new finding: SPN and P3 amplitudes are interrelated, reflecting relative attention allocation between tasks. In poor attention states, impaired attentional shifting and inhibitory control led to reduced P3 amplitude for the primary task and heightened SPN activity for the secondary task.","PeriodicalId":19213,"journal":{"name":"Neuroreport","volume":" ","pages":"412-420"},"PeriodicalIF":1.6,"publicationDate":"2025-05-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12043257/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144012786","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

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