New biotechnology最新文献

Biodegradable Zein/PEG Nanofibers Incorporated with Natural Antimicrobial Compounds for Eco-Friendly Food Packaging.

IF 4.5 2区生物学

New biotechnology Pub Date : 2025-03-18 DOI: 10.1016/j.nbt.2025.03.005

Pavel Pleva, Lucie Bartošová, Magda Janalíková, Martina Polášková, Alena Opálková Šišková, Lucie Matošková, Ondřej Krejčí, Jana Sedlaříková

{"title":"Biodegradable Zein/PEG Nanofibers Incorporated with Natural Antimicrobial Compounds for Eco-Friendly Food Packaging.","authors":"Pavel Pleva, Lucie Bartošová, Magda Janalíková, Martina Polášková, Alena Opálková Šišková, Lucie Matošková, Ondřej Krejčí, Jana Sedlaříková","doi":"10.1016/j.nbt.2025.03.005","DOIUrl":"https://doi.org/10.1016/j.nbt.2025.03.005","url":null,"abstract":"<p><p>Nanofibrous zein/PEG based membranes incorporated with natural antimicrobial compounds were fabricated by electrospinning method. Structural and thermal analysis of prepared nanofibers revealed that the applied processing technique did not significantly affect the structure of pristine zein polymer. Morphological characterization showed a higher degree of polydispersity in the fibers modified with eugenol, thymol, nisin, or their combinations, and an average fiber diameter in the range from 300 to 390nm. Nanofibrous samples with eugenol and thymol prevented the growth of Escherichia coli and Staphylococcus aureus, while the nisin or its mixtures with phenols proved a high antibacterial effect against Gram-positive Listeria ivanovii. Zein/PEG membranes with bioactive molecules significantly eliminated biofilm formation, with the most pronounced effect of zein/PEG/Eug/Thy combination. Biodegradability testing of bioactive membranes revealed no significant slowdown of degradation process in comparison to control sample. Zein/PEG hydrophilic nanofibers enriched with phenol/nisin combinations demonstrated a high potential for development of sustainable packaging to improve the shelf-life and quality of foods.</p>","PeriodicalId":19190,"journal":{"name":"New biotechnology","volume":" ","pages":""},"PeriodicalIF":4.5,"publicationDate":"2025-03-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143670328","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

How the past is shaping the future of life science: The influence of automation and AI on biology.

IF 4.5 2区生物学

New biotechnology Pub Date : 2025-03-15 DOI: 10.1016/j.nbt.2025.03.004

Carrie Cizauskas, Erika DeBenedictis, Pete Kelly

{"title":"How the past is shaping the future of life science: The influence of automation and AI on biology.","authors":"Carrie Cizauskas, Erika DeBenedictis, Pete Kelly","doi":"10.1016/j.nbt.2025.03.004","DOIUrl":"10.1016/j.nbt.2025.03.004","url":null,"abstract":"<p><p>Automation has been a transformative force for many industries, including manufacturing and chemistry. While the term traditionally referred to mechanical operations to produce physical objects, the definition has since expanded: 1) it can now mean both physical and/or information automation; and 2) it can now produce physical and/or conceptual outputs. While automation has yet to fully revolutionize life science research, much of which still relies on manual processes, we show that biology automation is the ultimate mixture of the concepts listed above - it involves automation of physical and data processing, and production of physical samples as well as conceptual data outputs. Here, we explore the history of automation and what it can - and cannot - teach us about the future of automated life science experimentation. We examine the current state of automated biology, its major successes, and the remaining barriers to wider adoption. Unlike in other fields, however, automation is reaching broader integration in life science at a time when both biology and AI are reaching their adolescence. At The Align Foundation, we are anticipating this change and hoping to leverage this inflection as a way to accelerate and democratize research. We anticipate that this novel combination of automation, AI, and life science learning will impact the trajectory of biological research, including the design and execution of high-throughput experiments and the analysis of resulting large-scale data.</p>","PeriodicalId":19190,"journal":{"name":"New biotechnology","volume":" ","pages":"1-11"},"PeriodicalIF":4.5,"publicationDate":"2025-03-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143649593","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Enzyme-assisted extraction and product profiling of fucosylated xyloglucan from industrial citrus pectin residues

IF 4.5 2区生物学

New biotechnology Pub Date : 2025-03-11 DOI: 10.1016/j.nbt.2025.03.003

Tessa L. Biel-Nielsen , Jimmy J.P. Sejberg , Anne S. Meyer , Jesper Holck

{"title":"Enzyme-assisted extraction and product profiling of fucosylated xyloglucan from industrial citrus pectin residues","authors":"Tessa L. Biel-Nielsen , Jimmy J.P. Sejberg , Anne S. Meyer , Jesper Holck","doi":"10.1016/j.nbt.2025.03.003","DOIUrl":"10.1016/j.nbt.2025.03.003","url":null,"abstract":"<div><div>Industrial pectin production from citrus peels involves extended, multi-step acidic extraction at elevated temperatures. Pectin quality can be improved by reducing the extraction time or number of steps, but this comes at the expense of yield. A decrease in pectin yield revenue may be compensated for by developing new usage of the partially depectinized citrus residues. One option is to capitalize on the fucosylated xyloglucans present in the residues. Thus, the aim of this work was to maximize gentle extraction of fucosylated xyloglucan from partially depectinized citrus peel residues of orange and lemon and examine the effects of multi-step extraction on pectin quality. To maintain the structural integrity of the xyloglucan, an enzyme-assisted approach was used to release the xyloglucan from the fibrous pectin extraction residues, with a focus on enzymatic cellulose degradation. The performance of four commercial cellulase preparations were compared (Cellic CTec2, Cellic CTec3, ENZECO CE3, and ENZECO Glucanase PF) through a multivariate approach. All cellulase preparations, being multicomponent enzyme blends, induced partial degradation of xyloglucan simultaneously with the cellulose depolymerization; the lowest dosage of Cellic CTec2 (100 µL/g DM, 40 °C, pH 7.0) resulted in the highest yields of solubilized fucosylated xyloglucans of 53 % and 39 % from the depectinized lemon and orange residues, respectively. Following the enzymatic extraction, membrane filtration outperformed alcohol precipitation in separating the solubilized xyloglucan oligosaccharides (< 2 kDa) from the simultaneously released high molecular weight rhamnogalacturonan I-like structures (500–10 kDa). The data provide new options for improved valorization of industrial depectinized citrus residues.</div></div>","PeriodicalId":19190,"journal":{"name":"New biotechnology","volume":"87 ","pages":"Pages 93-104"},"PeriodicalIF":4.5,"publicationDate":"2025-03-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143625482","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Exploring simultaneous production of poly(3-hydroxybutyrate) and exopolysaccharides in cyanobacteria-rich microbiomes

IF 4.5 2区生物学

New biotechnology Pub Date : 2025-03-11 DOI: 10.1016/j.nbt.2025.02.008

Beatriz Altamira-Algarra , Joan García , Cristiana A.V. Torres , Maria A.M. Reis , Eva Gonzalez-Flo

{"title":"Exploring simultaneous production of poly(3-hydroxybutyrate) and exopolysaccharides in cyanobacteria-rich microbiomes","authors":"Beatriz Altamira-Algarra , Joan García , Cristiana A.V. Torres , Maria A.M. Reis , Eva Gonzalez-Flo","doi":"10.1016/j.nbt.2025.02.008","DOIUrl":"10.1016/j.nbt.2025.02.008","url":null,"abstract":"<div><div>The aim of this study was to explore the viability of the dual production of poly(3-hydroxybutyrate) (PHB) and exopolysaccharides (EPS) by seven microbiomes rich in cyanobacteria. Our initial experiments involved to screen for EPS-producing candidates and examine the impact of salinity and acetate on EPS synthesis. Salinity's known influence on EPS production and acetate's role in enhancing PHB production guided our parameter selection. Surprisingly, neither the introduction of an external carbon source (acetate) nor exposure to an abiotic stressor (salt) significantly altered EPS synthesis rates, which ranged from 25 to 150 mg·L<sup>−1</sup>, or its composition, with glucose being the dominant sugar component. Scaling up to a 3 L photobioreactor, we achieved simultaneous biopolymer production, reaching up to 205 mg·L<sup>−1</sup> EPS and 87 mg·L<sup>−1</sup> PHB. Additionally, the presence of uronic acid in the EPS facilitated biomass flocculation, streamlining the separation process, and potentially reducing associated time and costs.</div></div>","PeriodicalId":19190,"journal":{"name":"New biotechnology","volume":"87 ","pages":"Pages 82-92"},"PeriodicalIF":4.5,"publicationDate":"2025-03-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143625484","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

OncoFlow: A multiplexed microfluidic platform for personalized drug sensitivity assessment.

IF 4.5 2区生物学

New biotechnology Pub Date : 2025-03-10 DOI: 10.1016/j.nbt.2025.03.002

Matan Krasner, Efrat Barbiro-Michaely, Ulrike Bening Abu-Shach, Amir Onn, Limor Broday, Doron Gerber

{"title":"OncoFlow: A multiplexed microfluidic platform for personalized drug sensitivity assessment.","authors":"Matan Krasner, Efrat Barbiro-Michaely, Ulrike Bening Abu-Shach, Amir Onn, Limor Broday, Doron Gerber","doi":"10.1016/j.nbt.2025.03.002","DOIUrl":"10.1016/j.nbt.2025.03.002","url":null,"abstract":"<p><p>While biomarker-guided treatments and NGS-based approaches are refining precision medicine, they are not universally applicable. The gap between the genomic characterization of tumors and their functional behavior is becoming increasingly evident. There is an escalating demand for functional assays that can customize cancer treatments for individual patients and bridge this gap. We have developed OncoFlow, an integrated microfluidic platform that automates viability assays. This platform customizes treatment options by assessing the functional responses of a patient's tumor cells to a specific drug panel. This study specifically addressed non-small cell lung adenocarcinoma (NSCLC) in patients presenting pleural effusion. We used the NCI-H2228 adenocarcinoma cell line, which harbors the EML4-ALK fusion oncogene, to develop and fine-tune the viability assay. Cells cultivated in microfluidic chambers were treated with various concentrations of the tyrosine kinase inhibitors alectinib and crizotinib, and the cytotoxic effects were measured. The results were consistent with those from conventional cell culture methods, thereby validating the assay's reliability. Next, pleural effusion samples from six NSCLC patients, four of them harboring the EML4-ALK rearrangement were tested with alectinib and crizotinib using the OncoFlow system. Monitoring and analysis of cell viability showed varied sensitivities to crizotinib, while all samples exhibited resistance to alectinib. These findings underscore OncoFlow's potential to enhance physician decision-making and customize treatment plans, ultimately improving patient outcomes.</p>","PeriodicalId":19190,"journal":{"name":"New biotechnology","volume":" ","pages":"105-111"},"PeriodicalIF":4.5,"publicationDate":"2025-03-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143616485","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Applicability of a laccase from the eucalypt wood endophytic fungus Hormonema sp. CECT-13092 for advanced bioethanol production 桉树木材内生真菌 Hormonema sp. CECT-13092 的漆酶在高级生物乙醇生产中的适用性。

IF 4.5 2区生物学

New biotechnology Pub Date : 2025-03-07 DOI: 10.1016/j.nbt.2025.03.001

Luisa García-Fuentevilla, María E. Eugenio, Raquel Martín-Sampedro, David Ibarra

{"title":"Applicability of a laccase from the eucalypt wood endophytic fungus Hormonema sp. CECT-13092 for advanced bioethanol production","authors":"Luisa García-Fuentevilla, María E. Eugenio, Raquel Martín-Sampedro, David Ibarra","doi":"10.1016/j.nbt.2025.03.001","DOIUrl":"10.1016/j.nbt.2025.03.001","url":null,"abstract":"<div><div>This work studies, for the first time, the potential of a laccase from the endophyte fungus <em>Hormonema</em> sp. CECT-13092, compared to a laccase from the saprophyte fungus <em>Trametes villosa</em>, for delignification and detoxification of steam-exploded eucalypt to improve subsequent bioethanol production. Regarding laccase delignification, the use of <em>Hormonema</em> sp. and <em>T. villosa</em> laccases did not show evidence of delignification of steam-exploded material, and rather low glucose and xylose recoveries were obtained during saccharification assays of laccase-treated samples compared with their respective controls. With regard to laccase detoxification, the reduction of the total phenolic inhibitors content presents in steam-exploded material by both laccases (phenols removal of 47 % and 60 % by <em>Hormonema</em> sp. and <em>T. villosa</em> laccases<em>,</em> respectively), triggered the fermentation by <em>Saccharomyces cerevisiae</em> of laccase-treated samples when 0.2 g L<sup>−1</sup> of inoculum was used during a simultaneous saccharification and fermentation (SSF) process. Moreover, when the inoculum was increased from 0.2 to 1.0 g L<sup>−1</sup>, both laccases shortened the yeast lag phase during the SSF process. Then, faster glucose consumption and ethanol production rates (ethanol productivity values of 0.25 and 0.28 g L<sup>−1</sup> h<sup>−1</sup> for <em>T. villosa</em> and <em>Hormonema</em> sp. laccases, respectively, compared to 0.02 g L<sup>−1</sup> h<sup>−1</sup> for control samples) were noticed. This fact proves the high potential of this new entophytic fungal laccase for bioethanol production enhancement, comparable to commercial laccases.</div></div>","PeriodicalId":19190,"journal":{"name":"New biotechnology","volume":"87 ","pages":"Pages 60-71"},"PeriodicalIF":4.5,"publicationDate":"2025-03-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143586427","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Utilization of lactose and whey permeate for the sustainable production of polyhydroxyalkanoates by Hydrogenophaga pseudoflava DSM1034

IF 4.5 2区生物学

New biotechnology Pub Date : 2025-03-03 DOI: 10.1016/j.nbt.2025.02.009

Lorenzo Favaro , Sergio Casella , Elettra Parro , Giuliana Franzosi , Valentina Rodighiero , Marina Basaglia

{"title":"Utilization of lactose and whey permeate for the sustainable production of polyhydroxyalkanoates by Hydrogenophaga pseudoflava DSM1034","authors":"Lorenzo Favaro , Sergio Casella , Elettra Parro , Giuliana Franzosi , Valentina Rodighiero , Marina Basaglia","doi":"10.1016/j.nbt.2025.02.009","DOIUrl":"10.1016/j.nbt.2025.02.009","url":null,"abstract":"<div><div>The utilization of agri-food wastes holds significant importance from both environmental and economic standpoints. Whey permeate, a by-product of cheese manufacturing with high lactose content, could be considered a promising substrate for microbial growth to yield value-added products. Whey permeate was therefore investigated as a potential feedstock for the production of polyhydroxyalkanoates (PHAs) by <em>Hydrogenophaga pseudoflava</em> DSM1034. Although the ability of this microorganism to grow and accumulate PHAs on whey permeate has been previously demonstrated, the PHAs yields were notably low. Therefore, a meticulous inoculum optimization was performed, taking into consideration adaptation to lactose-containing substrates, initial bacterial concentration, and growth kinetics. In media containing lactose as a carbon source, the lag phase, exceeding 40 hours in non-optimized conditions, decreased to only 4 hours. In whey permeate, a cell dry weight (CDW) of 5.49 ± 0.08 g/L was obtained. PHAs fraction in CDW and PHAs yield were 52.48 ± 3.14 % of CDW and 0.144 g/g of consumed sugar, respectively. Furthermore, these results were achieved in flask without hydrolysis of lactose contained in the permeate. Moreover, no galactose remained unutilized in the spent broth demonstrating that <em>H. pseudoflava</em> DSM1034 metabolized both sugars contained in lactose. Additionally, in culture media containing mixtures of glucose and galactose, microbial growth was lower compared to growth on comparable amounts of lactose. This study underscores the potential of whey permeate as a valuable resource for the production of PHAs, offering a sustainable solution for the utilization of dairy industry by-products, mitigating its environmental impact and promoting sustainable resource utilization.</div></div>","PeriodicalId":19190,"journal":{"name":"New biotechnology","volume":"87 ","pages":"Pages 72-81"},"PeriodicalIF":4.5,"publicationDate":"2025-03-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143567767","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

From the lab to the field and closer to the market: Production of the biopolymer cyanophycin in plants

IF 4.5 2区生物学

New biotechnology Pub Date : 2025-02-28 DOI: 10.1016/j.nbt.2025.02.006

Jana Huckauf , Ursula Weisenfeld , Inge Broer

{"title":"From the lab to the field and closer to the market: Production of the biopolymer cyanophycin in plants","authors":"Jana Huckauf , Ursula Weisenfeld , Inge Broer","doi":"10.1016/j.nbt.2025.02.006","DOIUrl":"10.1016/j.nbt.2025.02.006","url":null,"abstract":"<div><div>A range of studies has investigated the production of biopolymers in plants but a comprehensive assessment of feasibility and environmental safety and consumer acceptance is lacking. This review delivers such an assessment. It describes the establishment of the production in tobacco and potato, the analysis of lead events in the greenhouse and in the field, the establishment and upscaling of effective isolation processes and storage conditions, taking the cyanobacterial storage peptide cyanophycin (CGP) as an example. The paper lists several industrial and medical applications of CGP and its building blocks Arg-Asp-dipeptides. This production is especially interesting because the CGP content can exceed 10 % of the dry weight (dw) in the greenhouse and still deliver 4 g per plant in the field. Furthermore, risk assessment of CGP production in potatoes <em>in vitro</em>, <em>in vivo</em>, in the greenhouse, and in the field showed no relevant differences concerning environment or consumer safety compared with the near isogenic control. A consumer choice analysis in four European countries showed a preference for biodegradable CGP in food-wrapping materials over conventional plastic wrapping. Although data on economic feasibility is lacking, CGP as a renewable, biodegradable and CO<sub>2</sub>-neutrally produced compound, is preferable over fossil fuels in many applications.</div></div>","PeriodicalId":19190,"journal":{"name":"New biotechnology","volume":"87 ","pages":"Pages 29-38"},"PeriodicalIF":4.5,"publicationDate":"2025-02-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143520650","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Microalgal biorefineries in sustainable biofuel production and other high-value products

IF 4.5 2区生物学

New biotechnology Pub Date : 2025-02-27 DOI: 10.1016/j.nbt.2025.02.007

Swapnamoy Dutta , Sampriti Kataki , Ishita Banerjee , Cheryl Bernice Pohrmen , Krishna Kumar Jaiswal , Amit K. Jaiswal

{"title":"Microalgal biorefineries in sustainable biofuel production and other high-value products","authors":"Swapnamoy Dutta , Sampriti Kataki , Ishita Banerjee , Cheryl Bernice Pohrmen , Krishna Kumar Jaiswal , Amit K. Jaiswal","doi":"10.1016/j.nbt.2025.02.007","DOIUrl":"10.1016/j.nbt.2025.02.007","url":null,"abstract":"<div><div>Microalgae has been emerging as a promising solution against the backdrop of the global need for sustainable, eco-friendly alternatives. This review article analyses the use of photosynthetic microalgae as an important resource for sustainable biofuel and high value bioproduct production, emphasizing the potential of self-sustaining microalgae biorefineries. A closed-loop, integrated multi-product producing microalgal biorefinery approach could significantly reduce the indicated negative environmental and energy impact from standalone microalgal biofuel generation. The economic feasibility of these biorefineries is linked to their recovery rate, improved by integrating various unit operations as well as multiple product dimensions under optimal conditions, enhancing resource recovery, process efficiency, and profitability. This approach ensures profitability and ubiquitous implementation of microalgal biorefineries, offering a sustainable solution to market demands. In conclusion, making microalgae biorefineries a major player in sustainable bioeconomy underscores the necessity of interdisciplinary research to surmount current challenges and completely realize their advantages.</div></div>","PeriodicalId":19190,"journal":{"name":"New biotechnology","volume":"87 ","pages":"Pages 39-59"},"PeriodicalIF":4.5,"publicationDate":"2025-02-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143537474","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Microfluidic method for rapidly determining the protein and lipid yield of microalgae

IF 4.5 2区生物学

New biotechnology Pub Date : 2025-02-21 DOI: 10.1016/j.nbt.2025.02.001

Shubhanvit Mishra , Ting-Yu Pan , Yi-Ju Liu , Chi-Shuo Chen , Da-Jeng Yao

{"title":"Microfluidic method for rapidly determining the protein and lipid yield of microalgae","authors":"Shubhanvit Mishra , Ting-Yu Pan , Yi-Ju Liu , Chi-Shuo Chen , Da-Jeng Yao","doi":"10.1016/j.nbt.2025.02.001","DOIUrl":"10.1016/j.nbt.2025.02.001","url":null,"abstract":"<div><div>Microalgae are a promising source of green energy. They produce valuable bioproducts, such as proteins and lipids, and remove atmospheric carbon. In this study, we developed a microfluidic chip for culturing and screening for microalgae with high protein and lipid contents. Over 30 microalgae cultures can be grown in parallel in hanging drops on the chip and separately subjected to various experimental treatments, such as random mutations or different light levels. The microfluidic chip has a simple design and was fabricated from optically transparent polymethyl methacrylate; it could be easily operated without complex equipment (e.g., a syringe pump). The design was experimentally validated by culturing Cyanidium sp. and performing in-situ fluorescein measurements of its protein content after various ultraviolet and illumination treatments; significant increases in protein yield were observed for selected mutants (approximately 36 %) and further increased with optimized illumination (2500 lux; approximately 35 %). Moreover, Botryococcus braunii cultured in hanging drops that were separately immobilized using a biocompatible agarose gel for in-situ Raman spectroscopy measurements that rapidly determined the lipid composition. Our developed single-drop Raman spectroscopy method not only quantified the lipid content but also revealed its detailed chemical composition. In summary, the designed microfluidic chip is suitable for in-situ fluorescent assays and Raman microspectroscopy and is a compelling solution for high-throughput screening of algal lipids and proteins, reducing the labor required for breeding mutant algae and optimizing algal cultivation.</div></div>","PeriodicalId":19190,"journal":{"name":"New biotechnology","volume":"87 ","pages":"Pages 20-28"},"PeriodicalIF":4.5,"publicationDate":"2025-02-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143482671","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0