New biotechnology最新文献

Systematic identification of genomic hotspots for high-yield protein production in CHO cells

IF 4.5 2区生物学

New biotechnology Pub Date : 2025-04-12 DOI: 10.1016/j.nbt.2025.04.006

Minouk Lee , Sung-Hyuk Han , Dongseok Kim , Seongtae Yun , Jinho Yeom , Minji Kyeong , Seo-Young Park , Dong-Yup Lee

{"title":"Systematic identification of genomic hotspots for high-yield protein production in CHO cells","authors":"Minouk Lee , Sung-Hyuk Han , Dongseok Kim , Seongtae Yun , Jinho Yeom , Minji Kyeong , Seo-Young Park , Dong-Yup Lee","doi":"10.1016/j.nbt.2025.04.006","DOIUrl":"10.1016/j.nbt.2025.04.006","url":null,"abstract":"<div><div>The efficient and stable production of therapeutic proteins in Chinese hamster ovary (CHO) cells hinges on robust cell line development (CLD). Traditional methods relying on random transgene integration often result in clonal variability, requiring extensive and resource-intensive screening. To address this limitation, we established a systematic, multiomics-driven framework that integrates 202 RNA-sequencing datasets and whole-genome sequencing data to identify genomic “hotspot” loci for precise and high-yield transgene integration. From an initial pool of 20 candidate loci, 5 top-performing hotspots were validated using site-specific integration in CHO-DG44 cells via the CRISPR/Cas9 system with Recombinase-mediated cassette exchange (RMCE). These genomic hotspots achieved 2.2- to 15.0-fold higher relative specific productivity compared to previously known controls (<em>Fer1L4</em> and <em>Locus1</em> sites), across multiple therapeutic proteins, including a lysosomal storage disorder-related enzyme and an Immunoglobulin G (IgG)-related monoclonal antibody (mAb) expression. This study offers a transformative approach to CLD, achieving significant improvements in productivity, genomic stability, and efficiency, as well as paving the way for enhanced biopharmaceutical manufacturing.</div></div>","PeriodicalId":19190,"journal":{"name":"New biotechnology","volume":"88 ","pages":"Pages 61-72"},"PeriodicalIF":4.5,"publicationDate":"2025-04-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143829313","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Valorization of waste cooking oil for bioproduction of industrially-relevant metabolites in Ashbya gossypii

IF 4.5 2区生物学

New biotechnology Pub Date : 2025-04-07 DOI: 10.1016/j.nbt.2025.04.002

Javier Martín-González , Javier-Fernando Montero-Bullón , Gloria Muñoz-Fernández , Rubén M. Buey , Alberto Jiménez

{"title":"Valorization of waste cooking oil for bioproduction of industrially-relevant metabolites in Ashbya gossypii","authors":"Javier Martín-González , Javier-Fernando Montero-Bullón , Gloria Muñoz-Fernández , Rubén M. Buey , Alberto Jiménez","doi":"10.1016/j.nbt.2025.04.002","DOIUrl":"10.1016/j.nbt.2025.04.002","url":null,"abstract":"<div><div>Waste cooking oil (WCO) is a byproduct of culinary processes, which undergoes degradation due to high temperatures during frying and cooking. Beyond its detrimental effects on health, including potential carcinogenic effects, WCO poses a significant environmental threat, emphasizing the need for urgent recycling efforts. Valorization of WCO as a carbon source for microbial fermentations emerges as a feasible alternative in a bioeconomy context. The aim of the present work is to explore the ability of <em>Ashbya gossypii</em>, a natural overproducer of riboflavin that is currently used in the industrial production of the vitamin, to exploit WCO for the production of industrially relevant metabolites such as riboflavin, folates, biolipids and monoterpenes. Our results demonstrate that WCO is an effective carbon source for <em>A. gossypii</em> bioproduction of riboflavin, folates and biolipids, reaching among the highest titers described so far in flask fermentation: riboflavin titer (312.5 mg/L) increased 4.8-fold compared to glucose-based medium; folate production reached 7.6 mg/L; and the intracellular lipids were above 80 % of the cell dry weight. In contrast, the production of the monoterpenes limonene and sabinene was not improved with the utilization of WCO. Taken together, our results present a proof-of-principle for the implementation of a novel bioprocess for the valorization of WCO using the industrial fungus <em>A. gossypii</em>.</div></div>","PeriodicalId":19190,"journal":{"name":"New biotechnology","volume":"88 ","pages":"Pages 32-38"},"PeriodicalIF":4.5,"publicationDate":"2025-04-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143791937","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Screening assay for polyester hydrolyzing microorganisms using fluorescence-labeled poly(butylene adipate) 利用荧光标记聚己二酸丁二醇酯筛选聚酯水解微生物的检测方法。

IF 4.5 2区生物学

New biotechnology Pub Date : 2025-04-05 DOI: 10.1016/j.nbt.2025.03.007

Bettina Semler , Karin Binder , Doris Ribitsch , Alessandro Pellis , Georg M. Guebitz

{"title":"Screening assay for polyester hydrolyzing microorganisms using fluorescence-labeled poly(butylene adipate)","authors":"Bettina Semler , Karin Binder , Doris Ribitsch , Alessandro Pellis , Georg M. Guebitz","doi":"10.1016/j.nbt.2025.03.007","DOIUrl":"10.1016/j.nbt.2025.03.007","url":null,"abstract":"<div><div>Despite recent advances, there is still a demand for more efficient enzymes hydrolyzing synthetic polymers. Automated high throughput screening strategies of microorganisms from different environments could yield novel enzymes but require specific methods for detection of polymer hydrolysis in complex matrices. Here, 5-carboxy-fluorescein (5-FAM) was covalently coupled to poly(butylene adipate) (PBA) and blended at 1 %, 5 % and 10 % w/w concentrations with non-labeled PBA. Hydrolysis of PBA by the Thc_Cut1 cutinase from <em>Thermobifida cellulosilytica</em> was confirmed via quantification of the released monomers 1,4-butanediol and adipic acid, weight loss and FTIR analysis. Upon incubation with Thc_Cut1, hydrolysis of all three fluorescent labeled PBA blends lead to a clear fluorescence increase of up to 4000 RFU while no signal change was detected for the blank and for heat-inactivated enzyme (signal below 500 RFU). In a next step, as a model organism <em>Pichia pastoris</em> expressing the identical cutinase was cultivated in the presences of labeled PBA. Despite the complex matrix, a fluorescence increase of up to 500 RFU was observed for <em>P. pastoris</em> expressing the enzyme while no significant signal change was seen for the control strain (lacking Thc_Cut1 expression). Likewise, extracellular enzymes from the fungi <em>Fusarium solani</em> and <em>Alternaria alternata</em> hydrolyzed labeled PBA leading to fluorescence increases of 1328 and 1187 RFU. This indicates that 5-FAM covalently coupled to polymers could be used for development of simple and high throughput screening platforms to identify polymer decomposing microorganisms and enzymes.</div></div>","PeriodicalId":19190,"journal":{"name":"New biotechnology","volume":"88 ","pages":"Pages 39-45"},"PeriodicalIF":4.5,"publicationDate":"2025-04-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143803799","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A distinct autofluorescence distribution pattern marks enzymatic deconstruction of plant cell wall

IF 4.5 2区生物学

New biotechnology Pub Date : 2025-04-05 DOI: 10.1016/j.nbt.2025.04.001

Solmaz Hossein Khani , Khadidja Ould Amer , Noah Remy , Berangère Lebas , Anouck Habrant , Ali Faraj , Grégoire Malandain , Gabriel Paës , Yassin Refahi

{"title":"A distinct autofluorescence distribution pattern marks enzymatic deconstruction of plant cell wall","authors":"Solmaz Hossein Khani , Khadidja Ould Amer , Noah Remy , Berangère Lebas , Anouck Habrant , Ali Faraj , Grégoire Malandain , Gabriel Paës , Yassin Refahi","doi":"10.1016/j.nbt.2025.04.001","DOIUrl":"10.1016/j.nbt.2025.04.001","url":null,"abstract":"<div><div>Achieving an economically viable transformation of plant cell walls into bioproducts requires a comprehensive understanding of enzymatic deconstruction. Microscale quantitative analysis offers a relevant approach to enhance our understanding of cell wall hydrolysis, but becomes challenging under high deconstruction conditions. This study comprehensively addresses the challenges of quantifying the impact of extensive enzymatic deconstruction on plant cell wall at microscale. Investigation of highly deconstructed spruce wood provided spatial profiles of cell walls during hydrolysis with remarkable precision. A distinct cell wall autofluorescence distribution pattern marking enzymatic hydrolysis along with an asynchronous impact of hydrolysis on cell wall structure, with cell wall volume reduction preceding cell wall accessible surface area decrease, were revealed. This study provides novel insights into enzymatic deconstruction of cell wall at under-investigated cell scale, and a robust computational pipeline applicable to diverse biomass species and pretreatment types for assessing hydrolysis impact and efficiency.</div></div>","PeriodicalId":19190,"journal":{"name":"New biotechnology","volume":"88 ","pages":"Pages 46-60"},"PeriodicalIF":4.5,"publicationDate":"2025-04-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143803784","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Enhancing 2,3-butanediol and acetoin production from brewer's spent grain hemicellulosic hydrolysate through bacterial co-cultivation

IF 4.5 2区生物学

New biotechnology Pub Date : 2025-03-24 DOI: 10.1016/j.nbt.2025.03.006

Juan C. López-Linares , Erlinda Rama , María Teresa García-Cubero , Mónica Coca , Caroline L. Perez , Celina K. Yamakawa , Giuliano Dragone , Solange I. Mussatto

{"title":"Enhancing 2,3-butanediol and acetoin production from brewer's spent grain hemicellulosic hydrolysate through bacterial co-cultivation","authors":"Juan C. López-Linares , Erlinda Rama , María Teresa García-Cubero , Mónica Coca , Caroline L. Perez , Celina K. Yamakawa , Giuliano Dragone , Solange I. Mussatto","doi":"10.1016/j.nbt.2025.03.006","DOIUrl":"10.1016/j.nbt.2025.03.006","url":null,"abstract":"<div><div>This study evaluated bacterial co-cultivation as a strategy to mitigate brewer’s spent grain (BSG) hemicellulosic hydrolysate toxicity, aiming to enhance 2,3-butanediol (2,3-BDO) and acetoin production through fermentation. Co-culture of <em>Paenibacillus polymyxa</em> with <em>Pseudomonas alloputida</em> or <em>Rhodococcus</em> sp. was assessed using synthetic medium and BSG hydrolysate. Attention was given to removing inhibitory compounds, including lignin-derived phenolics, hydroxymethylfurfural, furfural, and acetic acid, through microbial detoxification during co-cultivation. Various fermentation temperatures (30, 34, and 37 °C) and initial cell concentrations (OD<sub>600</sub> of 0.05 and 0.1) were tested. Both <em>P. polymyxa</em> and <em>Rhodococcus</em> sp. effectively removed inhibitory compounds present in the medium. Co-cultures with <em>Rhodococcus</em> sp. exhibited higher sugar consumption rates (1.01 vs 0.88 g/L·h) than <em>P. polymyxa</em> monoculture, efficiently utilizing glucose, xylose, and arabinose, producing 2,3-BDO and acetoin. In co-culture with <em>Rhodococcus</em> sp., concentration (3.7 g/L), yield (0.14 g/g) and productivity (0.10 g/L·h) of 2,3-BDO at 34 °C considerably surpassed that of the <em>P. polymyxa</em> monoculture, with an increase of up to 48 %. These findings highlight the potential of co-cultures, especially with <em>Rhodococcus</em> sp., to alleviate inhibitory compound impacts when using complex media for fermentation. This study represents the first exploration of 2,3-BDO and acetoin production from BSG hemicellulosic hydrolysates using co-cultures.</div></div>","PeriodicalId":19190,"journal":{"name":"New biotechnology","volume":"88 ","pages":"Pages 22-31"},"PeriodicalIF":4.5,"publicationDate":"2025-03-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143705413","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Biodegradable zein/PEG nanofibers incorporated with natural antimicrobial compounds for eco-friendly food packaging 加入天然抗菌化合物的可生物降解 Zein/PEG 纳米纤维用于生态友好型食品包装。

IF 4.5 2区生物学

New biotechnology Pub Date : 2025-03-18 DOI: 10.1016/j.nbt.2025.03.005

Pavel Pleva , Lucie Bartošová , Magda Janalíková , Martina Polášková , Alena Opálková Šišková , Lucie Matošková , Ondřej Krejčí , Jana Sedlaříková

{"title":"Biodegradable zein/PEG nanofibers incorporated with natural antimicrobial compounds for eco-friendly food packaging","authors":"Pavel Pleva , Lucie Bartošová , Magda Janalíková , Martina Polášková , Alena Opálková Šišková , Lucie Matošková , Ondřej Krejčí , Jana Sedlaříková","doi":"10.1016/j.nbt.2025.03.005","DOIUrl":"10.1016/j.nbt.2025.03.005","url":null,"abstract":"<div><div>Nanofibrous zein/PEG based membranes incorporated with natural antimicrobial compounds were fabricated by electrospinning method. Structural and thermal analysis of prepared nanofibers revealed that the applied processing technique did not significantly affect the structure of pristine zein polymer. Morphological characterization showed a higher degree of polydispersity in the fibers modified with eugenol, thymol, nisin, or their combinations, and an average fiber diameter in the range from 300 to 390 nm. Nanofibrous samples with eugenol and thymol prevented the growth of <em>Escherichia coli</em> and <em>Staphylococcus aureus</em>, while the nisin or its mixtures with phenols proved a high antibacterial effect against Gram-positive <em>Listeria ivanovii</em>. Zein/PEG membranes with bioactive molecules significantly eliminated biofilm formation, with the most pronounced effect of zein/PEG/Eug/Thy combination. Biodegradability testing of bioactive membranes revealed no significant slowdown of degradation process in comparison to control sample. Zein/PEG hydrophilic nanofibers enriched with phenol/nisin combinations demonstrated a high potential for development of sustainable packaging to improve the shelf-life and quality of foods.</div></div>","PeriodicalId":19190,"journal":{"name":"New biotechnology","volume":"88 ","pages":"Pages 12-21"},"PeriodicalIF":4.5,"publicationDate":"2025-03-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143670328","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

How the past is shaping the future of life science: The influence of automation and AI on biology

IF 4.5 2区生物学

New biotechnology Pub Date : 2025-03-15 DOI: 10.1016/j.nbt.2025.03.004

Carrie Cizauskas, Erika DeBenedictis, Pete Kelly

{"title":"How the past is shaping the future of life science: The influence of automation and AI on biology","authors":"Carrie Cizauskas, Erika DeBenedictis, Pete Kelly","doi":"10.1016/j.nbt.2025.03.004","DOIUrl":"10.1016/j.nbt.2025.03.004","url":null,"abstract":"<div><div>Automation has been a transformative force for many industries, including manufacturing and chemistry. While the term traditionally referred to mechanical operations to produce physical objects, the definition has since expanded: 1) it can now mean both physical and/or information automation; and 2) it can now produce physical and/or conceptual outputs. While automation has yet to fully revolutionize life science research, much of which still relies on manual processes, we show that biology automation is the ultimate mixture of the concepts listed above – it involves automation of physical and data processing, and production of physical samples as well as conceptual data outputs. Here, we explore the history of automation and what it can — and cannot — teach us about the future of automated life science experimentation. We examine the current state of automated biology, its major successes, and the remaining barriers to wider adoption. Unlike in other fields, however, automation is reaching broader integration in life science at a time when both biology and AI are reaching their adolescence. At The Align Foundation, we are anticipating this change and hoping to leverage this inflection as a way to accelerate and democratize research. We anticipate that this novel combination of automation, AI, and life science learning will impact the trajectory of biological research, including the design and execution of high-throughput experiments and the analysis of resulting large-scale data.</div></div>","PeriodicalId":19190,"journal":{"name":"New biotechnology","volume":"88 ","pages":"Pages 1-11"},"PeriodicalIF":4.5,"publicationDate":"2025-03-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143649593","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Enzyme-assisted extraction and product profiling of fucosylated xyloglucan from industrial citrus pectin residues

IF 4.5 2区生物学

New biotechnology Pub Date : 2025-03-11 DOI: 10.1016/j.nbt.2025.03.003

Tessa L. Biel-Nielsen , Jimmy J.P. Sejberg , Anne S. Meyer , Jesper Holck

{"title":"Enzyme-assisted extraction and product profiling of fucosylated xyloglucan from industrial citrus pectin residues","authors":"Tessa L. Biel-Nielsen , Jimmy J.P. Sejberg , Anne S. Meyer , Jesper Holck","doi":"10.1016/j.nbt.2025.03.003","DOIUrl":"10.1016/j.nbt.2025.03.003","url":null,"abstract":"<div><div>Industrial pectin production from citrus peels involves extended, multi-step acidic extraction at elevated temperatures. Pectin quality can be improved by reducing the extraction time or number of steps, but this comes at the expense of yield. A decrease in pectin yield revenue may be compensated for by developing new usage of the partially depectinized citrus residues. One option is to capitalize on the fucosylated xyloglucans present in the residues. Thus, the aim of this work was to maximize gentle extraction of fucosylated xyloglucan from partially depectinized citrus peel residues of orange and lemon and examine the effects of multi-step extraction on pectin quality. To maintain the structural integrity of the xyloglucan, an enzyme-assisted approach was used to release the xyloglucan from the fibrous pectin extraction residues, with a focus on enzymatic cellulose degradation. The performance of four commercial cellulase preparations were compared (Cellic CTec2, Cellic CTec3, ENZECO CE3, and ENZECO Glucanase PF) through a multivariate approach. All cellulase preparations, being multicomponent enzyme blends, induced partial degradation of xyloglucan simultaneously with the cellulose depolymerization; the lowest dosage of Cellic CTec2 (100 µL/g DM, 40 °C, pH 7.0) resulted in the highest yields of solubilized fucosylated xyloglucans of 53 % and 39 % from the depectinized lemon and orange residues, respectively. Following the enzymatic extraction, membrane filtration outperformed alcohol precipitation in separating the solubilized xyloglucan oligosaccharides (< 2 kDa) from the simultaneously released high molecular weight rhamnogalacturonan I-like structures (500–10 kDa). The data provide new options for improved valorization of industrial depectinized citrus residues.</div></div>","PeriodicalId":19190,"journal":{"name":"New biotechnology","volume":"87 ","pages":"Pages 93-104"},"PeriodicalIF":4.5,"publicationDate":"2025-03-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143625482","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Exploring simultaneous production of poly(3-hydroxybutyrate) and exopolysaccharides in cyanobacteria-rich microbiomes

IF 4.5 2区生物学

New biotechnology Pub Date : 2025-03-11 DOI: 10.1016/j.nbt.2025.02.008

Beatriz Altamira-Algarra , Joan García , Cristiana A.V. Torres , Maria A.M. Reis , Eva Gonzalez-Flo

{"title":"Exploring simultaneous production of poly(3-hydroxybutyrate) and exopolysaccharides in cyanobacteria-rich microbiomes","authors":"Beatriz Altamira-Algarra , Joan García , Cristiana A.V. Torres , Maria A.M. Reis , Eva Gonzalez-Flo","doi":"10.1016/j.nbt.2025.02.008","DOIUrl":"10.1016/j.nbt.2025.02.008","url":null,"abstract":"<div><div>The aim of this study was to explore the viability of the dual production of poly(3-hydroxybutyrate) (PHB) and exopolysaccharides (EPS) by seven microbiomes rich in cyanobacteria. Our initial experiments involved to screen for EPS-producing candidates and examine the impact of salinity and acetate on EPS synthesis. Salinity's known influence on EPS production and acetate's role in enhancing PHB production guided our parameter selection. Surprisingly, neither the introduction of an external carbon source (acetate) nor exposure to an abiotic stressor (salt) significantly altered EPS synthesis rates, which ranged from 25 to 150 mg·L<sup>−1</sup>, or its composition, with glucose being the dominant sugar component. Scaling up to a 3 L photobioreactor, we achieved simultaneous biopolymer production, reaching up to 205 mg·L<sup>−1</sup> EPS and 87 mg·L<sup>−1</sup> PHB. Additionally, the presence of uronic acid in the EPS facilitated biomass flocculation, streamlining the separation process, and potentially reducing associated time and costs.</div></div>","PeriodicalId":19190,"journal":{"name":"New biotechnology","volume":"87 ","pages":"Pages 82-92"},"PeriodicalIF":4.5,"publicationDate":"2025-03-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143625484","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

OncoFlow: A multiplexed microfluidic platform for personalized drug sensitivity assessment

IF 4.5 2区生物学

New biotechnology Pub Date : 2025-03-10 DOI: 10.1016/j.nbt.2025.03.002

Matan Krasner , Efrat Barbiro-Michaely , Ulrike Bening Abu-Shach , Amir Onn , Limor Broday , Doron Gerber

{"title":"OncoFlow: A multiplexed microfluidic platform for personalized drug sensitivity assessment","authors":"Matan Krasner , Efrat Barbiro-Michaely , Ulrike Bening Abu-Shach , Amir Onn , Limor Broday , Doron Gerber","doi":"10.1016/j.nbt.2025.03.002","DOIUrl":"10.1016/j.nbt.2025.03.002","url":null,"abstract":"<div><div>While biomarker-guided treatments and NGS-based approaches are refining precision medicine, they are not universally applicable. The gap between the genomic characterization of tumors and their functional behavior is becoming increasingly evident. There is an escalating demand for functional assays that can customize cancer treatments for individual patients and bridge this gap. We have developed OncoFlow, an integrated microfluidic platform that automates viability assays. This platform customizes treatment options by assessing the functional responses of a patient's tumor cells to a specific drug panel. This study specifically addressed non-small cell lung adenocarcinoma (NSCLC) in patients presenting pleural effusion. We used the NCI-H2228 adenocarcinoma cell line, which harbors the EML4-ALK fusion oncogene, to develop and fine-tune the viability assay. Cells cultivated in microfluidic chambers were treated with various concentrations of the tyrosine kinase inhibitors alectinib and crizotinib, and the cytotoxic effects were measured. The results were consistent with those from conventional cell culture methods, thereby validating the assay's reliability. Next, pleural effusion samples from six NSCLC patients, four of them harboring the EML4-ALK rearrangement were tested with alectinib and crizotinib using the OncoFlow system. Monitoring and analysis of cell viability showed varied sensitivities to crizotinib, while all samples exhibited resistance to alectinib. These findings underscore OncoFlow's potential to enhance physician decision-making and customize treatment plans, ultimately improving patient outcomes.</div></div>","PeriodicalId":19190,"journal":{"name":"New biotechnology","volume":"87 ","pages":"Pages 105-111"},"PeriodicalIF":4.5,"publicationDate":"2025-03-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143616485","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0