New biotechnology最新文献

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tiRNA: An efficient and controllable gene silencing technology via translation inhibition. tiRNA:一种有效、可控的翻译抑制基因沉默技术。
IF 4.9 2区 生物学
New biotechnology Pub Date : 2025-11-25 Epub Date: 2025-08-06 DOI: 10.1016/j.nbt.2025.07.010
Bei Xia, Jiajing Cai, Zhilin He, Qubo Zhu
{"title":"tiRNA: An efficient and controllable gene silencing technology via translation inhibition.","authors":"Bei Xia, Jiajing Cai, Zhilin He, Qubo Zhu","doi":"10.1016/j.nbt.2025.07.010","DOIUrl":"10.1016/j.nbt.2025.07.010","url":null,"abstract":"<p><p>RNA-targeted therapies have emerged as a revolutionary breakthrough in biomedicine recently, offering unprecedented precision in regulating gene expression. Among these, steric blocking oligonucleotides (SBOs) represent a unique class of therapeutics that function through a steric blocking mechanism, allowing for reversible effects without RNA degradation. However, designing SBOs is challenging due to the lack of universal guidelines and the need for detailed analysis of mRNA/pre-mRNA characteristics, key site distributions, and RNA-binding protein interactions. To address this, we developed a novel aptamer-based technology called translation inhibition RNA (tiRNA), which inhibits mRNA translation by linking an eIF4G-targeting aptamer to a reverse complementary sequence of the target gene's 5'-UTR. This approach leverages the typical translation initiation mechanism of mRNA and employs a straightforward design strategy to specifically target and inhibit the translation of selected mRNAs without affecting others or causing RNA degradation. The efficacy of tiRNA is comparable to that of siRNA, providing precision, safety, and controllability for treating diseases linked to protein overexpression. Moreover, the effects of tiRNA can be reversed using a specially designed neutralizing strand, restoring normal mRNA translation and enhancing treatment controllability and personalization. This method holds significant potential for applications in cancer, gene therapy, and other fields requiring precise regulation of protein expression.</p>","PeriodicalId":19190,"journal":{"name":"New biotechnology","volume":" ","pages":"177-190"},"PeriodicalIF":4.9,"publicationDate":"2025-11-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144799697","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Site-specifically immobilized d-amino acid dehydrogenase for the synthesis of d-phenylalanine. 定点固定化d-氨基酸脱氢酶合成d-苯丙氨酸。
IF 4.9 2区 生物学
New biotechnology Pub Date : 2025-09-30 DOI: 10.1016/j.nbt.2025.09.006
Krisztina Boros, András-Ernő Iszlai, Laura-Edit Barabás, László Csaba Bencze, Monica Ioana Toşa
{"title":"Site-specifically immobilized d-amino acid dehydrogenase for the synthesis of d-phenylalanine.","authors":"Krisztina Boros, András-Ernő Iszlai, Laura-Edit Barabás, László Csaba Bencze, Monica Ioana Toşa","doi":"10.1016/j.nbt.2025.09.006","DOIUrl":"https://doi.org/10.1016/j.nbt.2025.09.006","url":null,"abstract":"<p><p>Aromatic d-amino acids (d-AAs) have gained increasing attention as chiral building blocks, with biocatalytic procedures emerging as powerful methods for their asymmetric synthesis. d-Amino acid dehydrogenases (DAADH), developed by protein engineering from meso-diaminopimelate dehydrogenases, step out as highly efficient biocatalysts for the reductive amination-based production of d-AAs. Enzyme immobilization allows the recovery and reuse of biocatalysts, while also enhances their operational stability, which is essential for industrial applications. Since the immobilization of DAADHs have been less explored, we targeted to further progress within the covalent immobilization of DAADH from Ureibacillus thermosphaericus, by exploring its site-specific, covalent immobilization. The individual replacement of several surficial Ser residues to Cys at positions 2, 58, 92, 185, 192 and 317 of UtDAADH, allowed their site-specific immobilization onto the maleimide-functionalized Purolite® ECR8415F methacrylic support. The highest specific activity values, ~0.078 and ~0.083 U/mg provided by immobilization through Cys2 and Cys192, respectively, showed 2.1- and 2.2-fold higher values compared to the covalently, but non-specifically immobilized UtDAADH. The recyclability of the immobilized preparations was tested among 10 reductive amination-cycles of phenylpyruvate and based on the retained conversion and specific activities, UtDAADH immobilized through S2C was the best-performing biocatalyst, maintaining 65-70% conversion and 50% of the initial activity after the 10<sup>th</sup> cycle. After the assessment of optimal enzyme/substrate ratio, the immobilized S2C UtDAADH was tested in three consecutive 200 mg-scale reaction, providing the enantiopure d-Phe with complete conversions and excellent >88% isolation yields, supporting its synthetic applicability.</p>","PeriodicalId":19190,"journal":{"name":"New biotechnology","volume":" ","pages":""},"PeriodicalIF":4.9,"publicationDate":"2025-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145213212","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Effect of salinity on the composition of a seawater-adapted strain of Scenedesmus almeriensis 盐度对一株适应海水环境的绿僵菌组成的影响
IF 4.9 2区 生物学
New biotechnology Pub Date : 2025-09-25 DOI: 10.1016/j.nbt.2025.09.008
Elia Rivera-Sánchez , Silvia Villaró-Cos , María Salinas-García , Francisco Javier Alarcón , Marco García-Vaquero , Lucie K. Tintrop , Daniel Kurpan , Tomás Lafarga
{"title":"Effect of salinity on the composition of a seawater-adapted strain of Scenedesmus almeriensis","authors":"Elia Rivera-Sánchez ,&nbsp;Silvia Villaró-Cos ,&nbsp;María Salinas-García ,&nbsp;Francisco Javier Alarcón ,&nbsp;Marco García-Vaquero ,&nbsp;Lucie K. Tintrop ,&nbsp;Daniel Kurpan ,&nbsp;Tomás Lafarga","doi":"10.1016/j.nbt.2025.09.008","DOIUrl":"10.1016/j.nbt.2025.09.008","url":null,"abstract":"<div><div>The main objective of this study was to evaluate the potential adaptation to seawater of the freshwater strain <em>Scenedesmus almeriensis</em> and to evaluate the effect of salinity on its growth, morphology, and biochemical composition. Incorporating low seawater concentrations into the culture medium (up to 103 mM NaCl) resulted in an increase in biomass productivity from 0.15 to 0.22 g·L<sup>-¹</sup> ·day<sup>-¹</sup> and an increase in the maximum specific growth rate from 0.14 to 0.26 day<sup>-¹</sup>. This was attributed to the presence of micronutrients in the seawater and adaptive responses to stress. Despite a lower biomass productivity (0.11 g·L<sup>−1</sup>·day<sup>-¹</sup>), <em>Scenedesmus almeriensis</em> was able to grow well (0.15 day<sup>−1</sup>) in a medium formulated with only seawater and commercial fertilisers. Cell morphology was significantly affected, with a 150 % increase in cell perimeter and an increase in roundness from 61.5 % (freshwater) to 95.8 % (seawater). The use of seawater also affected the chemical composition of the biomass. Seawater favoured the synthesis of specific fatty acids that have nutritional and industrial value, including polyunsaturated fatty acids. The protein content was slightly reduced under saline conditions but remained at 40 % (which is high compared to other biomasses). The results highlight the potential of seawater as a sustainable and cost-effective substitute for freshwater to produce <em>Scenedesmus almeriensis</em>. Future studies will validate the production of these strains at the large scale and identify potential industrial uses for the biomass produced.</div></div>","PeriodicalId":19190,"journal":{"name":"New biotechnology","volume":"90 ","pages":"Pages 114-121"},"PeriodicalIF":4.9,"publicationDate":"2025-09-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145154160","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
The diversity and keystone species of cucumber rhizosphere microbiome: Unveiling their role in driving cucumber growth and microbial communities 黄瓜根际微生物群落的多样性和关键物种:揭示其在黄瓜生长和微生物群落中的作用
IF 4.9 2区 生物学
New biotechnology Pub Date : 2025-09-24 DOI: 10.1016/j.nbt.2025.09.007
Yaping Yin , Raja Asad Ali Khan , Liping Li , Yinggu Wu , Chun Li , Yuejian Li , Sen Ren , Jing Zhang , Weiwei Wang , Runmao Lin , Manman Zhang , Xiaojun Liu , Jumei Hou , Genyun Liang
{"title":"The diversity and keystone species of cucumber rhizosphere microbiome: Unveiling their role in driving cucumber growth and microbial communities","authors":"Yaping Yin ,&nbsp;Raja Asad Ali Khan ,&nbsp;Liping Li ,&nbsp;Yinggu Wu ,&nbsp;Chun Li ,&nbsp;Yuejian Li ,&nbsp;Sen Ren ,&nbsp;Jing Zhang ,&nbsp;Weiwei Wang ,&nbsp;Runmao Lin ,&nbsp;Manman Zhang ,&nbsp;Xiaojun Liu ,&nbsp;Jumei Hou ,&nbsp;Genyun Liang","doi":"10.1016/j.nbt.2025.09.007","DOIUrl":"10.1016/j.nbt.2025.09.007","url":null,"abstract":"<div><h3>Background</h3><div>Studying the interrelationship between agroecosystems and the microbiome is essential in achieving sustainable agricultural development. In particular, the relationship between the rhizosphere microbiome and crops is critical to the health and stability of agroecosystems. This study evaluated the diversity and keystone species of the cucumber rhizosphere microbiome and investigated their contributing role in cucumber growth and microbial community regulation. A total of 108 soil samples were collected from the rhizosphere and bulk soil of cucumber crops in different locations and under different cultivation modes in a high-yield area. These samples were analyzed to study the interrelationships between different cultivation modes and crop rhizosphere microorganisms.</div></div><div><h3>Results</h3><div>The results revealed significant regional differences in microbial communities due to microhabitat (rhizosphere and bulk soil) and geographic location. Common bacteria such as <em>Pseudomonas</em>, <em>Sphingomonas</em>, <em>Lysobacter</em> and <em>Rhodanobacter</em> were the most abundant and showed strong correlations with several other bacteria through co-occurrence networking. In addition, <em>Flavobacterium</em> sp. and <em>Bacillus</em> sp. were identified as keystone species for the rhizobia community structure. Synthetic microbial communities and pot experiments confirmed that these two keystone species could enrich the soil microbime and promote cucumber growth. <em>Flavobacterium</em> sp. may promote cucumber growth by regulating the rhizosphere microbial community while <em>Bacillus</em> sp. may influence cucumber growth by stabilizing the rhizosphere microbial biomass, further enhancing nutrient cycling efficiency and improving system stability, which in turn affected the agroecosystem.</div></div><div><h3>Conclusions</h3><div>Our study demonstrates that specific keystone species are important in shaping the cucumber rhizosphere microbiome and plant growth. These results provide insights into agroecosystem-microbe interrelationships and provide a potential basis for optimizing agricultural management strategies.</div></div>","PeriodicalId":19190,"journal":{"name":"New biotechnology","volume":"90 ","pages":"Pages 134-144"},"PeriodicalIF":4.9,"publicationDate":"2025-09-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145154725","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
How food wastes can be converted into new products: European legislation and analysis of enzymatic hydrolysis 如何将食物垃圾转化为新产品:欧洲立法和酶水解分析。
IF 4.9 2区 生物学
New biotechnology Pub Date : 2025-09-20 DOI: 10.1016/j.nbt.2025.09.005
Laura Esposito, Francesca Accardo, Barbara Prandi, Tullia Tedeschi
{"title":"How food wastes can be converted into new products: European legislation and analysis of enzymatic hydrolysis","authors":"Laura Esposito,&nbsp;Francesca Accardo,&nbsp;Barbara Prandi,&nbsp;Tullia Tedeschi","doi":"10.1016/j.nbt.2025.09.005","DOIUrl":"10.1016/j.nbt.2025.09.005","url":null,"abstract":"<div><div>The generated food waste has a significant economic and environmental impact. Since most of this is considered nutrient-rich substrate, it can be reduced or converted to avoid negative effects. In recent years, new technologies have increasingly focused on this aim by extracting and recovering valuable components for the formulation of new products. Herein, this review analyses food waste management strategies and emerging scientific advancements. Among these, enzymatic hydrolysis represents a promising sustainable alternative to traditional chemical extraction. Unlike chemical methods, it operates under mild conditions, reducing energy consumption and harmful by-products, while efficiently recovering fibres, proteins, phenolic compounds, and other biomolecules of interest from food waste. Furthermore, its effectiveness can be significantly enhanced when combined with other techniques. However, most of these applications are currently at the laboratory scale, and a thorough assessment of the potential benefits and the feasibility at an industrial level is required. In this context, integrating enzymatic hydrolysis within circular economy models can further improve resource efficiency by promoting waste valorisation in industrial applications. This approach aligns with sustainable development goals, fostering the creation of bio-based products and reducing dependence on non-renewable resources. Despite facing challenges such as regulatory constraints and the need for scalable, cost-effective processes, the development of innovative and sustainable practices can bring significant economic, social, and environmental benefits.</div></div>","PeriodicalId":19190,"journal":{"name":"New biotechnology","volume":"90 ","pages":"Pages 122-133"},"PeriodicalIF":4.9,"publicationDate":"2025-09-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145125093","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Prenylation of diverse indole derivatives by the fungal aromatic prenyltransferase RePT 真菌芳香戊烯基转移酶RePT对多种吲哚衍生物的戊烯化反应。
IF 4.9 2区 生物学
New biotechnology Pub Date : 2025-09-16 DOI: 10.1016/j.nbt.2025.09.002
Pimvisuth Chunkrua, Mirjam A. Kabel, Jean-Paul Vincken, Willem J.H. van Berkel, Wouter J.C. de Bruijn
{"title":"Prenylation of diverse indole derivatives by the fungal aromatic prenyltransferase RePT","authors":"Pimvisuth Chunkrua,&nbsp;Mirjam A. Kabel,&nbsp;Jean-Paul Vincken,&nbsp;Willem J.H. van Berkel,&nbsp;Wouter J.C. de Bruijn","doi":"10.1016/j.nbt.2025.09.002","DOIUrl":"10.1016/j.nbt.2025.09.002","url":null,"abstract":"<div><div>Prenylation is a widespread natural modification of compounds that serves to functionalize and often enhance the bioactivity of plant and microbial secondary metabolites, including indole derivatives. In this study, we aimed to expand the library of prenylated indoles using RePT, a fungal (i.e. <em>Rasamsonia emersonii</em>) aromatic prenyltransferase from the dimethylallyl tryptophan synthase (DMATS) family. Previous work showed that RePT readily <em>C</em>7- and <em>N</em>1-prenylated <span>l</span>-tryptophan, and <em>O</em>-prenylated <span>l</span>-tyrosine and a number of phenolic stilbenes. Here, we investigated its regioselectivity further with 23 indole substrates, including tryptophan derivatives with varying <em>C</em>4-<em>C</em>7 substituents and several <em>C</em>3-substituted indoles. High conversion was observed primarily with fluorinated tryptophans and unsubstituted indole. Product analysis by UHPLC-PDA-ESI-MS<sup>n</sup> and NMR revealed that RePT mainly catalyzed either normal prenylation at <em>C</em>7 or reverse prenylation at <em>N</em>1 on a series of halogenated tryptophans. The regioselectivity observed for several substrates was strongly influenced by the position of the halogen substituent, particularly fluorine, which displayed its characteristic <em>ortho-</em>/<em>para-</em>directing effect. In the absence of the amino acid moiety, RePT’s regioselectivity in some cases shifted from its typical preference, leading to prenylation at alternative positions such as <em>C</em>3 and <em>C</em>6. These findings showcase the versatility of RePT for modifying diverse indole derivatives and demonstrate, for the first time, halogen-induced steering of the regioselectivity of DMATS to facilitate synthesis of bioactive prenylated compounds and intermediates.</div></div>","PeriodicalId":19190,"journal":{"name":"New biotechnology","volume":"90 ","pages":"Pages 88-96"},"PeriodicalIF":4.9,"publicationDate":"2025-09-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145086626","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Expanding the quorum sensing toolbox: Promoter libraries and hybrid promoter for dynamic genetic circuits 扩展群体感应工具箱:动态遗传电路的启动子库和混合启动子。
IF 4.9 2区 生物学
New biotechnology Pub Date : 2025-09-12 DOI: 10.1016/j.nbt.2025.09.004
Jasmine De Baets, Irene Parmentier, Brecht De Paepe, Marjan De Mey
{"title":"Expanding the quorum sensing toolbox: Promoter libraries and hybrid promoter for dynamic genetic circuits","authors":"Jasmine De Baets,&nbsp;Irene Parmentier,&nbsp;Brecht De Paepe,&nbsp;Marjan De Mey","doi":"10.1016/j.nbt.2025.09.004","DOIUrl":"10.1016/j.nbt.2025.09.004","url":null,"abstract":"<div><div>The interesting features of quorum sensing systems make them very appealing for synthetic biology applications. The first steps have already been taken in characterizing these systems to accelerate their successful implementation. In this work, we explore the next step, tuning. Multiple strategies exist for tuning genetic circuits and quorum sensing systems in general, where the most straightforward solution is to vary the expression level of the synthase or transcription factor. However, these tuning possibilities can be broadened by expanding the variety of quorum sensing-regulated promoters. Here, we expand the range of tuning possibilities by constructing promoter libraries for P<sub>lasI</sub> (LasI/LasR system) and P<sub>esaR/PesaS</sub> (EsaI/EsaR system), enabling direct modulation of downstream gene expression levels. Next, the quorum sensing synthetic biology toolbox was further expanded with the creation of a new-to-nature genetic part, the hybrid promoter, which requires two autoinducers for activation, namely 3-oxo-hexanoyl- and 3-oxo-dodecanoyl-homoserine lactone. This promoter was optimized and applied to design a multistage genetic switch capable of creating a growth stage followed by two sequential production stages. Additionally, the circuit was optimized by incorporating the new promoter variants created in this study. This complex genetic circuit demonstrates the true potential of these quorum sensing systems for achieving various cell-density related dynamic circuits.</div></div>","PeriodicalId":19190,"journal":{"name":"New biotechnology","volume":"90 ","pages":"Pages 97-113"},"PeriodicalIF":4.9,"publicationDate":"2025-09-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145058526","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
The Filterprep: A simple and efficient approach for high-yield, high-purity plasmid DNA purification Filterprep:一种简单有效的高产、高纯度质粒DNA纯化方法
IF 4.9 2区 生物学
New biotechnology Pub Date : 2025-09-12 DOI: 10.1016/j.nbt.2025.09.003
Yu-Hsuan Cheng , Yu-Jiu Wu , Yung-Chun Shih , Yu-Qian Lin , Yu-Wei Chang , Yu-Heng Wu , En-Wei Hu , Ren-Hsuan Ku , Cheng-Mu Wu , Shao-Chi Wu , Ting-Yu Yeh , Chung-Te Chang
{"title":"The Filterprep: A simple and efficient approach for high-yield, high-purity plasmid DNA purification","authors":"Yu-Hsuan Cheng ,&nbsp;Yu-Jiu Wu ,&nbsp;Yung-Chun Shih ,&nbsp;Yu-Qian Lin ,&nbsp;Yu-Wei Chang ,&nbsp;Yu-Heng Wu ,&nbsp;En-Wei Hu ,&nbsp;Ren-Hsuan Ku ,&nbsp;Cheng-Mu Wu ,&nbsp;Shao-Chi Wu ,&nbsp;Ting-Yu Yeh ,&nbsp;Chung-Te Chang","doi":"10.1016/j.nbt.2025.09.003","DOIUrl":"10.1016/j.nbt.2025.09.003","url":null,"abstract":"<div><div>Plasmid DNA purification remains a fundamental yet resource-intensive step in molecular biology and biotechnology. Conventional protocols often yield plasmids with suboptimal purity, while commercial kits, though efficient, are costly and use proprietary formulations with limited transparency for troubleshooting or customization. Here, we present Filterprep, a hybrid method combining classical ethanol precipitation with a single spin-column cleanup step, enabling rapid recovery of high-purity plasmid DNA with yields notably higher than those obtained using commercial kits, in approximately 40 min, with yields up to 5-fold greater than those of representative commercial midiprep kits under matched conditions. Filterprep offers a transparent, cost-effective, and scalable alternative, simplifying workflows and reducing hands-on time without compromising DNA quality. The purified plasmids are compatible with a variety of downstream applications, including mammalian cell transfection, protein expression, and reporter assays, while remaining stable for 12 months at −20 °C. With its balance of efficiency and practicality, Filterprep is well suited for high-throughput laboratories and resource-limited settings.</div></div>","PeriodicalId":19190,"journal":{"name":"New biotechnology","volume":"90 ","pages":"Pages 77-87"},"PeriodicalIF":4.9,"publicationDate":"2025-09-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145060003","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Effect of culture medium composition and reuse on the growth of Porphyridium cruentum 培养基组成及重复使用对黄斑紫菜生长的影响
IF 4.9 2区 生物学
New biotechnology Pub Date : 2025-09-11 DOI: 10.1016/j.nbt.2025.09.001
Florencia Cáceres-Ferroni , María Salinas-García , Silvia Villaró-Cos , Elia Rivera-Sánchez , Tomás Lafarga
{"title":"Effect of culture medium composition and reuse on the growth of Porphyridium cruentum","authors":"Florencia Cáceres-Ferroni ,&nbsp;María Salinas-García ,&nbsp;Silvia Villaró-Cos ,&nbsp;Elia Rivera-Sánchez ,&nbsp;Tomás Lafarga","doi":"10.1016/j.nbt.2025.09.001","DOIUrl":"10.1016/j.nbt.2025.09.001","url":null,"abstract":"<div><div>The aim of this study was to develop a fertiliser-based culture medium to reduce production costs and to enhance the sustainability of producing <em>Porphyridium cruentum</em>, a red marine microalga of commercial interest. Additionally, the impact of water recirculation on microalgal growth was assessed. Overall, the results indicated that the nitrogen source significantly affected biomass growth, with sodium nitrate supporting higher biomass productivity (0.23 g·L<sup>−1</sup>·day<sup>−1</sup>), surpassing sodium nitrite (0.18 g·L<sup>−1</sup>·day<sup>−1</sup>) and ammonium chloride (0.14 g·L<sup>−1</sup>·day<sup>−1</sup>). Urea had a negative impact on growth. A N:P molar ratio of 20:1 increased biomass productivity by approximately 24 % compared to the lower ratio studied (8:1) while also reducing phosphorus demand. The optimal medium composition was: 1.75 g·L<sup>−1</sup> NaNO<sub>3</sub>, 0.23 g·L<sup>−1</sup> K<sub>2</sub>HPO<sub>4</sub>·3 H<sub>2</sub>O, 0.04 g·L<sup>−1</sup> CaCl<sub>2</sub>, 0.49 g·L<sup>−1</sup> MgSO<sub>4</sub>·7 H<sub>2</sub>O, and 24.0 mg·L<sup>−1</sup> Karentol®. Lastly, water reutilisation negatively impacted biomass concentration, promoting the accumulation of extracellular organic carbon and bacteria as well as increasing the viscosity and turbidity of the culture.</div></div>","PeriodicalId":19190,"journal":{"name":"New biotechnology","volume":"90 ","pages":"Pages 65-76"},"PeriodicalIF":4.9,"publicationDate":"2025-09-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145045397","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Proximity-based site-specific labeling of a native IgG Fab fragment by a fusion microbial transglutaminase-protein G variant 融合微生物转谷氨酰胺酶蛋白G变体对天然IgG Fab片段的邻近位点特异性标记
IF 4.9 2区 生物学
New biotechnology Pub Date : 2025-08-21 DOI: 10.1016/j.nbt.2025.08.004
Koki Murozono , Riko Nishioka , Yoshirou Kawaguchi , Michio Kimura , Noriho Kamiya
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