New biotechnology最新文献

筛选
英文 中文
PHB in cyanobacteria: Analyzing production through images processing and FT-IR techniques. 蓝藻中的PHB:通过图像处理和FT-IR技术分析生产。
IF 4.5 2区 生物学
New biotechnology Pub Date : 2025-07-17 DOI: 10.1016/j.nbt.2025.07.003
Paula Villar Sola, Juan Fernández Montenegro, Sandra Iglesias Moreira, Francisco Rodríguez Lorenzo, Philippe Vandervorst, Erika Pancorbo González, Miguel Placer Lorenzo, Inés Pérez Couñago, Santiago Muíños Landín, Luz Herrero Castilla, Julio Illade Quinteiro, Juan A Álvarez Rodríguez, Beatriz Altamira Algarra, Eva Gonzalez Flo, Joan García
{"title":"PHB in cyanobacteria: Analyzing production through images processing and FT-IR techniques.","authors":"Paula Villar Sola, Juan Fernández Montenegro, Sandra Iglesias Moreira, Francisco Rodríguez Lorenzo, Philippe Vandervorst, Erika Pancorbo González, Miguel Placer Lorenzo, Inés Pérez Couñago, Santiago Muíños Landín, Luz Herrero Castilla, Julio Illade Quinteiro, Juan A Álvarez Rodríguez, Beatriz Altamira Algarra, Eva Gonzalez Flo, Joan García","doi":"10.1016/j.nbt.2025.07.003","DOIUrl":"https://doi.org/10.1016/j.nbt.2025.07.003","url":null,"abstract":"<p><p>Cyanobacteria have gained significant attention in recent years due to their ability to produce a variety of valuable compounds. One such compound is polyhydroxybutyrate (PHB), a biodegradable polymer with immense potential in various industrial applications. Given that PHB is stored intracellularly, a dedicated process is needed to extract and measure the biopolymer content. Nevertheless, this process is time consuming and requires environmental hazardous chemicals, such as chloroform. In the present work, we present two complementary methods developed to analyze and quantify PHB production in cyanobacteria microbiomes. The first one consists in an image processing applied on images obtained from Transmission Electronic Microscope (TEM), that can potentially be applied to others type of microscope images as confocal, for qualitative assessment. In this case, a segmentation process allows differentiating PHB grains inside cyanobacteria cells. A metric is then established by computing pixels area taken up by PHB in the whole image and in cyanobacteria cells. A good correlation (higher than 0.65) is observed for all indicators as regard to PHB content. The second method relies on Fourier-transform infrared (FTIR) spectroscopy, as a non-destructive and rapid method to analyze PHB. Absorption peaks due to carbonyl, and Amide I and II group characteristics of monomer structure in PHB and cyanobacteria´s protein are observed. A correlation coefficient r<sup>2</sup> of 0.96 is reached with the linear regression. A comparison between the two techniques is presented and their main advantages for PHB production optimization are explained. ------------------------------------------------------------------------------------------------------------------------.</p>","PeriodicalId":19190,"journal":{"name":"New biotechnology","volume":" ","pages":""},"PeriodicalIF":4.5,"publicationDate":"2025-07-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144668091","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Conditional guide RNA deactivation by mRNA and small molecule triggers in Saccharomyces cerevisiae. 酿酒酵母菌mRNA和小分子触发器的条件引导RNA失活。
IF 4.5 2区 生物学
New biotechnology Pub Date : 2025-07-16 DOI: 10.1016/j.nbt.2025.07.004
Chenggang Xi, Stephen Chiu, William E Voje, James M Carothers, Tae Seok Moon
{"title":"Conditional guide RNA deactivation by mRNA and small molecule triggers in Saccharomyces cerevisiae.","authors":"Chenggang Xi, Stephen Chiu, William E Voje, James M Carothers, Tae Seok Moon","doi":"10.1016/j.nbt.2025.07.004","DOIUrl":"https://doi.org/10.1016/j.nbt.2025.07.004","url":null,"abstract":"<p><p>CRISPR interference (CRISPRi) technologies have revolutionized bioengineering by providing precise tools for gene expression modulation, enabling targeted gene perturbation and metabolic pathway optimization. Despite these advances, achieving dynamic control over gene expression by CRISPR-based regulation remains a challenge due to its inherently static nature. Utilizing toehold-mediated strand displacement and ligand-responsive ribozymes (aptazymes), this study introduces switchable guide RNAs (gRNAs) that facilitate tunable gene expression mediated by mRNA or small molecule signals. We demonstrate complete silencing of gRNA via strategically designed 5' or 3' extensions that impede the gRNA spacer or the dCas9 handle, with subsequent restoration of function through sequestration or cleavage of the obstructive sequence. The resulting toehold-embedded or aptazyme-embedded gRNAs can be deactivated by specific signals, including two full-length translatable mRNAs and two small molecule triggers, thereby lifting CRISPRi repression on targeted genes. This modular approach allows for gRNA-based biocomputing through multi-layer or multi-input genetic logic gates in Saccharomyces cerevisiae. Offering a versatile strategy for post-CRISPR regulation in response to environmental signals or cellular states, this methodology expands the toolkit in eukaryotic systems for reversible control of gene expression.</p>","PeriodicalId":19190,"journal":{"name":"New biotechnology","volume":" ","pages":""},"PeriodicalIF":4.5,"publicationDate":"2025-07-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144668090","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Model-based engineering of Bacillus methanolicus towards de novo polyamine bioproduction from methanol 甲醇芽孢杆菌以甲醇为原料重新生产多胺的模型工程。
IF 4.5 2区 生物学
New biotechnology Pub Date : 2025-07-11 DOI: 10.1016/j.nbt.2025.07.001
Luciana Fernandes Brito , Adshaha Arampu , Fernando Pérez-García , Fatma Ece Altınışık Kaya , Nihat Alpagu Sayar , Berna Sariyar Akbulut , Trygve Brautaset
{"title":"Model-based engineering of Bacillus methanolicus towards de novo polyamine bioproduction from methanol","authors":"Luciana Fernandes Brito ,&nbsp;Adshaha Arampu ,&nbsp;Fernando Pérez-García ,&nbsp;Fatma Ece Altınışık Kaya ,&nbsp;Nihat Alpagu Sayar ,&nbsp;Berna Sariyar Akbulut ,&nbsp;Trygve Brautaset","doi":"10.1016/j.nbt.2025.07.001","DOIUrl":"10.1016/j.nbt.2025.07.001","url":null,"abstract":"<div><div>This study explores the one-carbon feedstock methanol to bolster sustainable bioproduction of valuable polyamines. <em>Bacillus methanolicus</em> MGA3, a methylotroph, stands out as a promising host due to its aptitude for employing methanol to synthesize various chemicals. Our approach used flux balance analysis (FBA) to leverage native <em>B. methanolicus</em> pathways for biosynthesis of the polyamines putrescine and spermidine. Despite possessing the genetic repertoire required for their production, <em>B. methanolicus</em> naturally secretes spermidine but not putrescine. Therefore, we created recombinant strains overexpressing endogenous and heterologous genes for putrescine biosynthesis via the arginine decarboxylase pathway, including arginine decarboxylase (<em>speA</em>) and agmatinase (<em>speB</em>). The <em>B. methanolicus</em> strain PUTEc, overexpressing <em>speAB</em> from <em>Escherichia coli</em> rather than native ones, achieved putrescine production of 47.5 ± 0.8 μM in shake flasks. Towards spermidine production, FBA pointed to overexpressing <em>S</em>-adenosylmethionine decarboxylase (<em>speH</em>) and spermidine synthase (<em>speE</em>) in the PUTEc strain. As a result, production of 83.9 ± 2.7 μM spermidine was achieved from methanol. Subsequently, the PUTEc strain underwent FBA-based screening involving precursor supplementation extracellularly in the growth medium or intracellularly by gene co-overexpression to enhance putrescine production. Overexpression of the endogenous ornithine biosynthesis pathway in the PUTEc strain yielded the highest methanol-based polyamine production in this study: 137.7 ± 1.8 μM putrescine in small-scale shake flask conditions. With further improvements in titer towards several grams per litre, this sustainable bioprocess could supply the steadily growing ∼ $500 M putrescine market. To our knowledge, this is the first proof-of-concept study towards production of putrescine and spermidine from methanol.</div></div>","PeriodicalId":19190,"journal":{"name":"New biotechnology","volume":"89 ","pages":"Pages 91-104"},"PeriodicalIF":4.5,"publicationDate":"2025-07-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144626786","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Hac1p-based inverse secretory pathway engineering (Hi-SPE) of Pichia pastoris for improved glucose oxidase production 基于hac1p的毕赤酵母反分泌途径工程(Hi-SPE)提高葡萄糖氧化酶产量。
IF 4.5 2区 生物学
New biotechnology Pub Date : 2025-07-10 DOI: 10.1016/j.nbt.2025.07.002
Xinyu Zhao , Jiayu Fang , Sijie Yu , Guoxia Liu , Yanping Zhang , Yin Li , Taicheng Zhu
{"title":"Hac1p-based inverse secretory pathway engineering (Hi-SPE) of Pichia pastoris for improved glucose oxidase production","authors":"Xinyu Zhao ,&nbsp;Jiayu Fang ,&nbsp;Sijie Yu ,&nbsp;Guoxia Liu ,&nbsp;Yanping Zhang ,&nbsp;Yin Li ,&nbsp;Taicheng Zhu","doi":"10.1016/j.nbt.2025.07.002","DOIUrl":"10.1016/j.nbt.2025.07.002","url":null,"abstract":"<div><div>Secretion and folding are common bottlenecks in protein expression using eukaryotic systems, and engineering the secretory pathway to enhance host cell capabilities is a key strategy for improving protein secretion. However, secretion is a very complex process, making the identification of likely targets for engineering a formidable task. In this study, using glucose oxidase (GOX) expression in <em>Pichia pastoris</em> (<em>Komagataella phaffii</em>) as a model, we introduce a strategy called Hac1p-based inverse secretory pathway engineering (Hi-SPE). This strategy leverages Hac1p, the actuator of the unfolded protein response, which is a naturally evolved mechanism to cope with protein overload in endoplasmic reticulum (ER) of eukaryotic cells. When combined with comparative transcriptomics, Hi-SPE narrows down the target from several hundred genes in traditional approaches to 20 secretion-related protein genes. Results showed that overexpression of six out of seven selected genes improved GOX secretion, including the co-chaperone, JEM1, which increased GOX expression per OD<sub>600</sub> by 147.6 %. Further optimization through combinatorial expression of secretion-related proteins led to a strain co-expressing <em>JEM1</em>, <em>KAR2</em>, and <em>CNE1</em>, achieving a GOX titer of 1903.2 U/mL in 1-L fed-batch fermentation. Additionally, transcriptomic analysis revealed the physiological effects of <em>JEM1</em> overexpression on <em>P</em>. <em>pastoris</em>. This study highlights Hi-SPE as a powerful strategy for improving protein secretion in eukaryotic systems.</div></div>","PeriodicalId":19190,"journal":{"name":"New biotechnology","volume":"89 ","pages":"Pages 82-90"},"PeriodicalIF":4.5,"publicationDate":"2025-07-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144619442","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Phenolic compounds fingerprints show distinct ligninolytic bacteria behaviours during technical lignins conversion 酚类化合物指纹在木质素技术转化过程中显示出不同的木质素分解细菌行为
IF 4.5 2区 生物学
New biotechnology Pub Date : 2025-06-28 DOI: 10.1016/j.nbt.2025.06.004
Corinne Ivaldi , Brigitte Chabbert , Roland Molinié , David Crônier , David Mathiron , Jean Xavier Fontaine , François Mesnard , Frédéric Velard , Harivony Rakotoarivonina
{"title":"Phenolic compounds fingerprints show distinct ligninolytic bacteria behaviours during technical lignins conversion","authors":"Corinne Ivaldi ,&nbsp;Brigitte Chabbert ,&nbsp;Roland Molinié ,&nbsp;David Crônier ,&nbsp;David Mathiron ,&nbsp;Jean Xavier Fontaine ,&nbsp;François Mesnard ,&nbsp;Frédéric Velard ,&nbsp;Harivony Rakotoarivonina","doi":"10.1016/j.nbt.2025.06.004","DOIUrl":"10.1016/j.nbt.2025.06.004","url":null,"abstract":"<div><div>Lignins, one of the main components of plant cell wall, and by-products of certain industries (paper and wood industries,…) are a renewable source of aromatic molecules. They can be degraded and transformed by microbial and enzymatic processes known to be respectful of the environment. Biological valorization of lignins remains challenging as biocatalysts are not sufficiently effective and efficient. Moreover, the chemical complexity and heterogeneity of lignins are a barrier to their use. Understanding the microbial behaviour on lignins by fingerprinting their efficient transformation could lead to the development of effective biological routes to valorise these aromatic polymers. Ligninolytic bacteria present some interesting features in term of ligninolytic enzymes productions, utilization of aromatic compounds <em>via</em> various intracellular pathways and the productions of molecules of interest from aromatic molecules. In this work, multiple approaches (growth studies, ligninolytic activities production, lignin modifications and phenolic compounds fingerprints) were used to understand the behaviour of two ligninolytic bacteria <em>Pandoraea norimbergensis</em> and <em>Comamonas composti</em>, in presence of lignins with different structures and origins. Results showed dissimilar growths profiles, lignin modifications, consumption and production of phenolic monomers and oligomers according to the bacteria and the lignin used. To achieve efficient transformation of lignins, suitable combination of biocatalysts and lignins is required and the microorganisms used must be selected on the basis of their metabolic capacity, and the structure and composition of lignin.</div></div>","PeriodicalId":19190,"journal":{"name":"New biotechnology","volume":"89 ","pages":"Pages 60-72"},"PeriodicalIF":4.5,"publicationDate":"2025-06-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144513731","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Single-cell proteins polyhydroxyalkanoates-rich microbial biomass from municipal and winery waste as potential additive for aquafeeds 从城市和酿酒厂废料中提取的富含多羟基烷酸盐的微生物生物量作为水产饲料的潜在添加剂
IF 4.5 2区 生物学
New biotechnology Pub Date : 2025-06-21 DOI: 10.1016/j.nbt.2025.06.003
Giulia Adele Tuci , Marco Gottardo , Aditi Parmar Chitharanjan , Gloriana Cardinaletti , Giulia Pascon , Paolo Pavan , Francesco Valentino
{"title":"Single-cell proteins polyhydroxyalkanoates-rich microbial biomass from municipal and winery waste as potential additive for aquafeeds","authors":"Giulia Adele Tuci ,&nbsp;Marco Gottardo ,&nbsp;Aditi Parmar Chitharanjan ,&nbsp;Gloriana Cardinaletti ,&nbsp;Giulia Pascon ,&nbsp;Paolo Pavan ,&nbsp;Francesco Valentino","doi":"10.1016/j.nbt.2025.06.003","DOIUrl":"10.1016/j.nbt.2025.06.003","url":null,"abstract":"<div><div>This study evaluated single-cell protein production from PHA-rich mixed microbial cultures obtained from fermentation and subsequent PHA storage, using urban (namely food waste and municipal sewage sludge; FW-MSS) and agricultural waste (namely wine lees; WL) streams as substrates. FW-MSS fermentation achieved stable short-chain fatty acid (SCFA) production and a high COD<sub>SCFA</sub>/COD<sub>SOL</sub> ratio of 0.77 ± 0.01, which allowed to select a mixed microbial culture (MMC) with intracellular PHA content of 15.1 wt%, which aligns with fish dietary standards and yielded a MMC biomass with a protein level of 55.1 wt% and a balanced essential amino acid (EAA) profile. In contrast, WL fermentation showed lower SCFA content and stability, yielding a MMC with 45.8 wt% of protein along with a high non-conformance rate (53.65 %), and 7.2 wt% PHA, making the resulting MMC more suited as a supplemental protein source. Distinct microbial communities developed in the two SBRs due to different feedstocks, influencing the abundance of PHA-storing bacteria, with no known fish pathogens detected in either sample. Statistical analysis confirmed FW-MSS’s superior product consistency, supporting its potential as a good quality SCP for aquafeed, especially for rainbow trout, as confirmed by its high essential amino acid index (EAAI).</div></div>","PeriodicalId":19190,"journal":{"name":"New biotechnology","volume":"89 ","pages":"Pages 29-39"},"PeriodicalIF":4.5,"publicationDate":"2025-06-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144366897","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
On-demand insulin manufacturing using cell-free systems with an "on-column" conversion approach 按需胰岛素制造使用无细胞系统与“柱上”转换方法。
IF 4.5 2区 生物学
New biotechnology Pub Date : 2025-06-20 DOI: 10.1016/j.nbt.2025.06.002
Shayan G. Borhani , Max Z. Levine , Chandrasekhar Gurramkonda , Yanyan Qu , Dingyin Tao , Christopher A. LeClair , James R. Swartz , Govind Rao
{"title":"On-demand insulin manufacturing using cell-free systems with an \"on-column\" conversion approach","authors":"Shayan G. Borhani ,&nbsp;Max Z. Levine ,&nbsp;Chandrasekhar Gurramkonda ,&nbsp;Yanyan Qu ,&nbsp;Dingyin Tao ,&nbsp;Christopher A. LeClair ,&nbsp;James R. Swartz ,&nbsp;Govind Rao","doi":"10.1016/j.nbt.2025.06.002","DOIUrl":"10.1016/j.nbt.2025.06.002","url":null,"abstract":"<div><div>Recent studies project that the prevalence of diabetes is expected to increase significantly and lead to escalating demand on the insulin supply chain. Despite being the first recombinant therapeutic approved by the FDA, insulin remains challenging to access for many around the globe. Here we report on advancements in manufacturing insulin using cell-free protein synthesis (CFPS) systems to rapidly produce mature desB30-insulin in less than a day. This is a major advance towards decentralizing insulin manufacturing and bringing production closer to the point-of-care, thereby improving diabetic patient accessibility. To this end, a purified cell-free extract, PUREfrex® 2.1, was utilized to synthesize a tagged proinsulin construct that can be captured and converted into mature insulin using an on-column affinity chromatography process. Notably, two chaperones, peptidyl prolyl isomerase (FkpA), and seven kilodalton protein (Skp) were observed to play a critical role during cell-free expression of proinsulin. The proinsulin was then immobilized on a Ni-NTA column where the purification and conversion of cell-free products were performed sequentially to yield desB30-insulin. Following further optimization, this method serves as a time and resource-efficient production process as compared to current methods. When applied simultaneously, the cell-free expression and on-column conversion methods reported here can be adopted to enable rapid on-demand insulin manufacturing in order to improve the accessibility of insulin and prevent future shortages.</div></div>","PeriodicalId":19190,"journal":{"name":"New biotechnology","volume":"89 ","pages":"Pages 51-59"},"PeriodicalIF":4.5,"publicationDate":"2025-06-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144369106","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Improving PHB production in cyanobacteria: Modeling the optimal light regime for growth 提高蓝藻中PHB的生产:模拟生长的最佳光照制度。
IF 4.5 2区 生物学
New biotechnology Pub Date : 2025-06-17 DOI: 10.1016/j.nbt.2025.05.005
I. Perez Couñago , J.M. Fernandez Montenegro , S. Iglesias Moreira , F. Rodriguez Lorenzo , M. Placer Lorenzo , P. Villar Sola , E. Pancorbo González , J. Illade Quinteiro , L. Herrero Castilla , J.A. Álvarez Rodríguez , B. Altamira Algarra , E. Gonzalez Flo , J. Garcia , F. Guedes , M. Lopez-Garcia , S. Muiños-Landin
{"title":"Improving PHB production in cyanobacteria: Modeling the optimal light regime for growth","authors":"I. Perez Couñago ,&nbsp;J.M. Fernandez Montenegro ,&nbsp;S. Iglesias Moreira ,&nbsp;F. Rodriguez Lorenzo ,&nbsp;M. Placer Lorenzo ,&nbsp;P. Villar Sola ,&nbsp;E. Pancorbo González ,&nbsp;J. Illade Quinteiro ,&nbsp;L. Herrero Castilla ,&nbsp;J.A. Álvarez Rodríguez ,&nbsp;B. Altamira Algarra ,&nbsp;E. Gonzalez Flo ,&nbsp;J. Garcia ,&nbsp;F. Guedes ,&nbsp;M. Lopez-Garcia ,&nbsp;S. Muiños-Landin","doi":"10.1016/j.nbt.2025.05.005","DOIUrl":"10.1016/j.nbt.2025.05.005","url":null,"abstract":"<div><div>The production of bioplastics, such as polyhydroxybutyrate (PHB), using cyanobacteria offers a sustainable alternative to conventional plastics. However, achieving economically viable production requires optimizing biomass growth. This study examined four growth models: Gompertz (empirical growth), Baranyi-Roberts (biologically dependent), Monod (nutrient dependent), and Aiba (irradiance dependent). The results indicate that a light-based model more accurately describes cyanobacterial growth and shows potential for optimizing light regimes. Additionally, an estimator was proposed to assess the potential PHB yield within the given biomass. Experiments were conducted to correlate photosynthetic efficiency with biomass production, providing deeper insights into the effects of light on growth. These findings support the development of optimized cultivation strategies, ultimately improving the economic viability of cyanobacteria-based bioplastics.</div></div>","PeriodicalId":19190,"journal":{"name":"New biotechnology","volume":"89 ","pages":"Pages 73-81"},"PeriodicalIF":4.5,"publicationDate":"2025-06-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144485268","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Effect of operating conditions on the microbial selection of P(3HB-co-3HV) producers in open continuous culture 开放式连续培养中操作条件对P(3HB-co-3HV)生产者微生物选择的影响
IF 4.5 2区 生物学
New biotechnology Pub Date : 2025-06-13 DOI: 10.1016/j.nbt.2025.06.001
Matteo Castiello , Laetitia Cavaillé , Mansour Bounouba , Simon Dubos , Claire Dumas , Etienne Paul
{"title":"Effect of operating conditions on the microbial selection of P(3HB-co-3HV) producers in open continuous culture","authors":"Matteo Castiello ,&nbsp;Laetitia Cavaillé ,&nbsp;Mansour Bounouba ,&nbsp;Simon Dubos ,&nbsp;Claire Dumas ,&nbsp;Etienne Paul","doi":"10.1016/j.nbt.2025.06.001","DOIUrl":"10.1016/j.nbt.2025.06.001","url":null,"abstract":"<div><div>From the perspective of producing P(3HB-co-3HV), the selection of PHA accumulating organisms from activated sludge with a feeding incorporating propionate was investigated in open continuous culture under dual carbon and phosphorus limitation conditions. The selected consortia were then harvested in batch cultures with phosphorus (P) deficiency to evaluate their PHA storage potential. Microbial selection exhibited consistent functional stability of PHA production, emphasizing the robustness of this enrichment strategy. A comparison with previous studies revealed that at a given degree of P limitation, the distribution of carbon consumption between cell growth, PHA storage and maintenance reactions was conditioned by the nature of the carbon source. The combination of the nature of the carbon substrate, the degree of P limitation and the dilution rate governs microbial competition. The dominant bacterial genera showed very heterogeneous PHA production capacities. <em>Acinetobacte</em>r spp., whose establishment was favored by the presence of acetate, a high dilution rate, and a low C/P ratio, was found to be a poor producer. In contrast, <em>Malikia</em> spp. and <em>Zoogloea</em> spp., mostly selected with butyrate, a lower dilution rate, and strong P limitation, exhibited high specific PHA production rates.</div></div>","PeriodicalId":19190,"journal":{"name":"New biotechnology","volume":"89 ","pages":"Pages 40-50"},"PeriodicalIF":4.5,"publicationDate":"2025-06-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144302586","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Tailored enzyme expression modifies Shewanella oneidensis biofilms and increases current density 量身定制的酶表达修饰希瓦氏菌生物膜和增加电流密度
IF 4.5 2区 生物学
New biotechnology Pub Date : 2025-06-09 DOI: 10.1016/j.nbt.2025.05.006
Edina Marlen Klein , Hannah Heintz , René Wurst , Christian Jonas Lapp , Miriam Edel , Hendrik Hähl , Karin Jacobs , Johannes Gescher
{"title":"Tailored enzyme expression modifies Shewanella oneidensis biofilms and increases current density","authors":"Edina Marlen Klein ,&nbsp;Hannah Heintz ,&nbsp;René Wurst ,&nbsp;Christian Jonas Lapp ,&nbsp;Miriam Edel ,&nbsp;Hendrik Hähl ,&nbsp;Karin Jacobs ,&nbsp;Johannes Gescher","doi":"10.1016/j.nbt.2025.05.006","DOIUrl":"10.1016/j.nbt.2025.05.006","url":null,"abstract":"<div><div>Biofilm formation is the most effective pathway for electron transfer to anodes in bioelectrochemical systems. However, the mechanisms triggering biofilm formation under anoxic conditions, as well as the architectural and compositional factors that positively influence current generation, are not well understood. Recent findings have shown that riboflavin can function similarly to a quorum sensing molecule in the γ-proteobacterium <em>Shewanella oneidensis</em>. Enhanced biofilm formation induced by riboflavin correlates with increased current densities. Only a limited number of candidate proteins were found to have altered concentrations due to this quorum sensing mechanism. This study demonstrates that the catalytic functions of the UDP-N-acetylglucosamine C4 epimerase WbpP and UDP-N-acetyl-D-glucosamine 6-dehydrogenase WbpA affect biofilm formation and lead to increased current density. Using optical coherence tomography, we found that the expression of each protein individually causes specific, quantifiable changes in biofilm architecture, including biovolume, height, and porosity. However, the current density did not significantly differ when these proteins were expressed alone compared to the control. In contrast, co-expression of WbpP and WbpA resulted in a doubling of current density, closely resembling the increases observed with riboflavin-mediated quorum sensing. We hypothesize that riboflavin-based quorum sensing may lead, through several intermediary steps, to the overproduction of WbpA and WbpP, resulting in better attachment to graphite anodes and thus higher current densities.</div></div>","PeriodicalId":19190,"journal":{"name":"New biotechnology","volume":"89 ","pages":"Pages 1-10"},"PeriodicalIF":4.5,"publicationDate":"2025-06-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144263229","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
0
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
相关产品
×
本文献相关产品
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:604180095
Book学术官方微信