Matteo Castiello , Laetitia Cavaillé , Mansour Bounouba , Simon Dubos , Claire Dumas , Etienne Paul
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引用次数: 0
Abstract
From the perspective of producing P(3HB-co-3HV), the selection of PHA accumulating organisms from activated sludge with a feeding incorporating propionate was investigated in open continuous culture under dual carbon and phosphorus limitation conditions. The selected consortia were then harvested in batch cultures with phosphorus (P) deficiency to evaluate their PHA storage potential. Microbial selection exhibited consistent functional stability of PHA production, emphasizing the robustness of this enrichment strategy. A comparison with previous studies revealed that at a given degree of P limitation, the distribution of carbon consumption between cell growth, PHA storage and maintenance reactions was conditioned by the nature of the carbon source. The combination of the nature of the carbon substrate, the degree of P limitation and the dilution rate governs microbial competition. The dominant bacterial genera showed very heterogeneous PHA production capacities. Acinetobacter spp., whose establishment was favored by the presence of acetate, a high dilution rate, and a low C/P ratio, was found to be a poor producer. In contrast, Malikia spp. and Zoogloea spp., mostly selected with butyrate, a lower dilution rate, and strong P limitation, exhibited high specific PHA production rates.
期刊介绍:
New Biotechnology is the official journal of the European Federation of Biotechnology (EFB) and is published bimonthly. It covers both the science of biotechnology and its surrounding political, business and financial milieu. The journal publishes peer-reviewed basic research papers, authoritative reviews, feature articles and opinions in all areas of biotechnology. It reflects the full diversity of current biotechnology science, particularly those advances in research and practice that open opportunities for exploitation of knowledge, commercially or otherwise, together with news, discussion and comment on broader issues of general interest and concern. The outlook is fully international.
The scope of the journal includes the research, industrial and commercial aspects of biotechnology, in areas such as: Healthcare and Pharmaceuticals; Food and Agriculture; Biofuels; Genetic Engineering and Molecular Biology; Genomics and Synthetic Biology; Nanotechnology; Environment and Biodiversity; Biocatalysis; Bioremediation; Process engineering.