New biotechnology最新文献

{"title":"Locus-specific quantification reveals dynamics of ribosomal ac4C modifications","authors":"Ningning Qin,&nbsp;Yin Wang,&nbsp;Qiaoling Yuan,&nbsp;Li Chen,&nbsp;Guo-Sheng Qu","doi":"10.1016/j.nbt.2026.01.003","DOIUrl":"10.1016/j.nbt.2026.01.003","url":null,"abstract":"<div><div>Eukaryotic ribosomes carry two evolutionarily conserved N4-acetylcytidine (ac4C) modifications within critical functional regions. However, fundamental questions remain regarding their deposition kinetics, potential dynamics, and functional interplay with other rRNA modifications. Here, using ac4C-Sanger, we quantitatively profiled ribosomal ac4C modifications across various rRNA precursors and in mature rRNA under varied conditions in <em>Saccharomyces cerevisiae</em>. We show that ac4C accumulates progressively during 18S rRNA maturation, with distinct kinetics at each site. Moreover, ribosomal ac4C levels are dynamic and respond to growth phase and thermal stress in a site-specific manner. Changes in modifications under heat stress are accompanied by alterations in translation fidelity and efficiency, although evidence is still lacking to establish a causal relationship. Genetic perturbation of the box C/D snoRNA snR40, which guides 2′-O-methylation Gm1271, revealed a differential effect on both ac4C sites, despite the spatial proximity of Gm1271 to only one of them. Beyond ac4C-Sanger, we also demonstrate that High-Resolution Melting (HRM) and SELECT (a single-base elongation- and ligation-based qPCR amplification method) can serve as orthogonal approaches to effectively detect and quantitatively measure ac4C at known sites. Collectively, our work establishes ribosomal ac4C as a dynamic epitranscriptomic mark, suggests intricate interdependencies governing its biogenesis, and provides accessible tools for its investigation, thereby underscoring the integrated and adaptive nature of the ribosomal modification landscape.</div></div>","PeriodicalId":19190,"journal":{"name":"New biotechnology","volume":"92 ","pages":"Pages 62-73"},"PeriodicalIF":4.9,"publicationDate":"2026-05-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145934410","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Microalgal cultivation on ricotta cheese whey: Modulating nutrient supply during cultivation to improve culture growth","authors":"Paolo Capano,&nbsp;Gaia Santini,&nbsp;Natascia Biondi,&nbsp;Giacomo Sampietro,&nbsp;Liliana Rodolfi","doi":"10.1016/j.nbt.2026.01.007","DOIUrl":"10.1016/j.nbt.2026.01.007","url":null,"abstract":"<div><div>Dairy industry generates significant effluent streams, comprising wastewaters and nutrient-rich by-products such as cheese whey, which pose environmental challenges for disposal, but also offer valorisation opportunities. Microalgae cultivation on whey represents a sustainable biotechnological approach that couples biomass production with effluent treatment. This study aimed at optimizing microalgal cultivation using ricotta cheese whey as substrate. Eighteen microalgal strains, mainly belonging to <em>Scenedesmus</em> and <em>Chlorella</em>, were initially screened for their ability to grow on whey. Selected strains were further tested in 300-mL photobioreactors under different management options: Low Whey Load (LWL), High Whey Load (HWL), Salinity and Micronutrient (S&amp;M), and Moderate Whey Load (MWL) trials. Results showed that most of the tested strains grew better when cultivated on diluted whey than on standard synthetic media, with increases from 56 to 590 % during the first days, and with high removal efficiencies. Several issues were identified during the trials, including nutrient shortage, salinity increase, micronutrients deficiency and potential inhibitory effects of whey compounds, which have hindered long term cultivation of microalgae on whey. In MWL trial, optimization of whey concentration (12 % v/v), replenishment strategy (30 % of the culture volume every 3-days), and micronutrient supplementation overcame these issues extending the cultivation period and improving biomass yield (203.6 billion cells L⁻¹ whey⁻¹ with the best strain). Overall, this work underscores the importance of carefully optimize microalgal cultivation management to maximise exploitation of dairy by-products and minimize growth-limiting effects of whey accumulation, thus offering a promising starting point for large-scale applications of whey in microalgae cultivation.</div></div>","PeriodicalId":19190,"journal":{"name":"New biotechnology","volume":"92 ","pages":"Pages 25-39"},"PeriodicalIF":4.9,"publicationDate":"2026-05-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145959356","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Zero-shot deep learning with multi-objective optimization improves thermostability of zearalenone hydrolase and xylanase","authors":"Fan Wu ,&nbsp;Rui Wu ,&nbsp;Linghui Chen ,&nbsp;Quan Chen ,&nbsp;Haiyan Liu","doi":"10.1016/j.nbt.2026.01.009","DOIUrl":"10.1016/j.nbt.2026.01.009","url":null,"abstract":"<div><div>Enhancing enzyme thermostability is crucial for industrial applications requiring robust performance under extreme conditions. Structure-based protein design models excel at improving thermal stability but often compromise enzymatic activity, while sequence-based models better preserve activity but struggle to enhance thermostability. It is challenging to efficiently generate multi-site mutants with both improved thermostability and intact activity using minimal experimental effort. Here, we used zearalenone hydrolase (RmZHD) and xylanase as model systems to evaluate different strategies for multi-site mutation design: (i) structure-based design with the ABACUS-R model, (ii) sequence-based design with the ProGen2 or MSA Transformer, (iii) integrated approaches combining either ProGen2 or MSA Transformer with ABACUS-R via Markov Chain Monte Carlo sampling with multi-objective scoring. Results showed that designing with ABACUS-R increased thermal stability by ∼15°C but caused 80 to 100 % activity loss. Sequence-based designs retained ∼19 % wild-type activity but failed to improve thermostability. Notably, zero-shot designs from integrating ABACUS-R with MSA Transformer achieved significant thermostability gains (<em>ΔT</em>_<em>m</em> ∼8°C) while preserving &gt; 95 % wild-type activity. This highlights the potential of combining sequence-and structure-based deep learning models for developing industrially relevant thermostable enzymes.</div></div>","PeriodicalId":19190,"journal":{"name":"New biotechnology","volume":"92 ","pages":"Pages 51-61"},"PeriodicalIF":4.9,"publicationDate":"2026-05-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146019193","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Ability of a microfluidic droplets system to assess the dynamics of Green Fluorescence Protein (GFP) production by Escherichia coli for modelling and bioprocess purposes","authors":"Hassan El Itawi ,&nbsp;Aya Zoghlami,&nbsp;Madeleine Charbonnier,&nbsp;Yusmel González-Hernández,&nbsp;Firas Ghali,&nbsp;Rafik Balti,&nbsp;Patrick Perré","doi":"10.1016/j.nbt.2026.01.006","DOIUrl":"10.1016/j.nbt.2026.01.006","url":null,"abstract":"<div><div>Understanding microbial growth and metabolism under controlled environments is critical for both fundamental research and bioprocess development. In this study, we present a cost-effective droplet-based microfluidic device enabling high-throughput screening of GFP expression in <em>Escherichia coli</em> (<em>E. coli</em>) under varying glucose concentrations. Fluorinated Ethylene Propylene (FEP) tubing was selected for its low cost and compatibility with stable water-in-oil emulsification, facilitating robust droplet generation. The system achieved a 33333-fold reduction in media consumption compared to traditional Erlenmeyer flask cultures. Growth kinetics and GFP expression were assessed in both Erlenmeyer flasks and microdroplets, showing high qualitative correlation between platforms. Low glucose levels (5–10 g/L) supported rapid initial growth and early GFP production, followed by a fluorescence decline due to nutrient depletion. In contrast, higher glucose concentrations (25–50 g/L) prolonged the exponential phase and enhanced GFP production per unit biomass, though growth was slowed by overflow metabolism. In microdroplets, delayed GFP expression at 25 and 50 g/L were observed, and parallel bioreactor experiments confirmed that this delay is caused by oxygen limitation at high glucose concentrations. Importantly, the microfluidic device enables controlled variation of oxygen availability simply by adjusting droplet size or generation frequency, providing a powerful means to probe oxygen-sensitive metabolic behaviors. These results validate the microfluidic platform’s ability to mimic Erlenmeyer flask-scale dynamics, while uniquely allowing precise modulation of oxygen transfer conditions at the microscale<strong>.</strong> The system offers a reliable, miniaturized alternative for optimizing microbial bioprocesses with drastically reduced reagent use and increased experimental throughput.</div></div>","PeriodicalId":19190,"journal":{"name":"New biotechnology","volume":"92 ","pages":"Pages 12-24"},"PeriodicalIF":4.9,"publicationDate":"2026-05-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145985253","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Utilization and valorization of lignin and lignin-derived compounds by Pseudomonas putida KT2440: A new role for glutathione peroxidase","authors":"Qing Zhou ,&nbsp;Annabel Fransen ,&nbsp;Paolo Innocenti ,&nbsp;Arthur F.J. Ram ,&nbsp;Johannes H. de Winde","doi":"10.1016/j.nbt.2026.01.002","DOIUrl":"10.1016/j.nbt.2026.01.002","url":null,"abstract":"<div><div>Lignin, a complex natural aromatic polymer, poses significant challenges to its efficient degradation, hindering the utilization of biomass for many industrial applications. Bacterial degradation of lignin may offer a promising solution to this challenge. This project aimed at elucidating the function of secreted oxidative enzymes from <em>Pseudomonas putida</em> involved in degradation and utilization of lignin and lignin-derived compounds. Using CRISPR-Cas9 and CRISPR-Cas3 systems, the putative lignin-degrading versatile peroxidase gene (VP; <em>PP</em>_<em>1686</em>, originally annotated as glutathione peroxidase GPx) and dye-decolorizing peroxidase gene (<em>PP_3248</em>) were individually knocked out from <em>P. putida</em> KT2440. The ∆PP_1686 mutant exhibited impaired growth and utilization of lignin-derived compounds. This correlated with reduced expression of p-hydroxybenzoate hydroxylase <em>pobA</em> and of DNA repair modules, alongside compensatory upregulation of energy and redox supply pathways. This work expands our knowledge on bacterial glutathione peroxidase by presenting a role beyond ROS scavenging. This work revealed the importance of <em>P. putida</em> VP/GPx in maintaining redox balance while supporting lignin-derived aromatic metabolism, offering new targets for future investigation into stress–metabolism crosstalk and lignin valorization strategies.</div></div>","PeriodicalId":19190,"journal":{"name":"New biotechnology","volume":"92 ","pages":"Pages 1-11"},"PeriodicalIF":4.9,"publicationDate":"2026-05-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145950227","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Harnessing design of experiments and automation significantly improves transient protein production in Chinese hamster ovary cells and accelerates drug discovery","authors":"Jing Ke ,&nbsp;Mandy Li ,&nbsp;Sultan Yilmaz,&nbsp;Yan Liu,&nbsp;Hang Thi Thu Nguyen,&nbsp;Pamela Zhang,&nbsp;Laurence Fayadat-Dilman,&nbsp;Jeanne E. Baker","doi":"10.1016/j.nbt.2026.01.010","DOIUrl":"10.1016/j.nbt.2026.01.010","url":null,"abstract":"<div><div>Chinese Hamster Ovary (CHO) cells produce the majority of approved therapeutic proteins and are the de facto platform for rapid, milligram-scale production via transient expression during early biologics discovery. However, performance is highly sensitive to transfection composition, feed strategy, and execution details; and vendor protocols often underperform without platform-specific optimization. We integrated Design of Experiments (DOE) with liquid-handling automation to develop a rapid, data-driven optimization workflow for CHO transient expression. Key factors and ranges were defined for an IgG1 expression and then extended to additional modalities, encompassing coding DNA, PEI transfection reagent, Feed A/B and glucose, inert filler DNA, and the DNA-PEI pipetting sequence that governs brief pre-complexing. Using Response Surface Methodology executed on an automated liquid handler, we generated structured datasets and fitted predictive models that accurately captured main effects, interactions, and curvature. The optimized conditions increased average volumetric productivity by approximately twofold, improved harvest viability, and reduced coding DNA input by about 70 % without detectable loss of product quality (SEC main-peak purity) within our tests. The settings transferred across multiple biologics modalities with consistent gains in titer. Automation eliminates variable‑volume manual pipetting in complex layouts, reduced handling errors, enabled precise short‑duration pre‑complexing steps, and accelerated model‑driven refinement. This approach shorten iteration cycles and de-risks early-stage process development for candidate therapeutic proteins.</div></div>","PeriodicalId":19190,"journal":{"name":"New biotechnology","volume":"92 ","pages":"Pages 40-50"},"PeriodicalIF":4.9,"publicationDate":"2026-05-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146019116","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Corrigendum to “More than meets the eye: Microalgal-bacterial association in polyhydroxybutyrate (PHB) accumulating cultures of Desmodesmus communis”[New Biotechnol 91 (2026), 192–200]","authors":"Martina Franchini ,&nbsp;Mara Simonazzi ,&nbsp;Federica Costantini ,&nbsp;Valentina Papa ,&nbsp;Rossella Pistocchi ,&nbsp;Stefano Ratti ,&nbsp;Chiara Samorì ,&nbsp;Laura Pezzolesi","doi":"10.1016/j.nbt.2026.01.013","DOIUrl":"10.1016/j.nbt.2026.01.013","url":null,"abstract":"","PeriodicalId":19190,"journal":{"name":"New biotechnology","volume":"92 ","pages":"Page 74"},"PeriodicalIF":4.9,"publicationDate":"2026-05-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146078932","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Precision glycoform engineering: Combining plant and in vitro systems for tailored biopharmaceutical production","authors":"Mijke R. Sweers,&nbsp;Ruud H.P. Wilbers,&nbsp;Arjen Schots,&nbsp;Pieter Nibbering","doi":"10.1016/j.nbt.2026.01.014","DOIUrl":"10.1016/j.nbt.2026.01.014","url":null,"abstract":"<div><div>Protein biopharmaceuticals play a key role in providing effective, targeted, and personalized therapies for diverse diseases, while also preventing and mitigating a broad range of infections. N-glycosylation is a key post-translational modification influencing the biological activity of many protein-based therapeutics, yet structure–function relationships of N-glycans remain poorly understood due to challenges in producing homogeneous glycoforms. Current go-to production hosts, mammalian and yeast cells, often yield heterogeneous glycan profiles and require extensive genetic manipulation. Alternative production hosts such as the plant <em>Nicotiana benthamiana</em>, provide more homogeneous glycosylation and flexibility through transient expression, but are limited in the generation of certain glycoforms. <em>In vitro</em> glycoengineering can overcome these limitations but is time consuming and requires expensive resources. In this study, we show that by combining <em>in planta</em> and <em>in vitro</em> glycoengineering strategies, we can quickly produce a wide range of homogeneous glycoforms of pharmaceutical proteins with high mannose, paucimannose, hybrid and complex N-glycan structures. Using <em>N. benthamiana</em> as a transient expression host, we produced two pharmaceutical glycoproteins — the monoclonal antibody rituximab and the helminth vaccine candidate OoASP-1 — and modified them <em>in vitro</em> using <em>Escherichia coli</em> produced glycoenzymes. The combination of these two glycoengineering systems minimizes the amount of time and resources required, while maintaining high glycan homogeneity. This scalable, flexible, and cost-effective platform opens the door to glycan structure–function relationship studies and can support rational design of next-generation biopharmaceuticals.</div></div>","PeriodicalId":19190,"journal":{"name":"New biotechnology","volume":"92 ","pages":"Pages 100-114"},"PeriodicalIF":4.9,"publicationDate":"2026-05-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146125709","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Advanced stable cell line development based on REMBAC (Rapid Efficient Manifold BACulovirus Transduction)","authors":"Sophie Huber,&nbsp;Sandra Diaz Sanchez,&nbsp;Gordana Wozniak-Knopp,&nbsp;Reingard Grabherr,&nbsp;Manuel Reithofer","doi":"10.1016/j.nbt.2026.01.011","DOIUrl":"10.1016/j.nbt.2026.01.011","url":null,"abstract":"<div><div>The BacMam platform is a scalable and efficient gene delivery system for mammalian cells, enabling the production of recombinant proteins or highly complex structures like bionanoparticles. This study represents the first comprehensive investigation to evaluate the performance of the BacMam platform in a common producer cell line HEK293–6E, on stable transgene expression mediated by the REMBAC (rapid efficient manifold baculovirus transduction) cassette. In this study, six transient high expression cassettes were adopted for stable gene expression and evaluated in HEK293–6E and Vero cells. The constructs included either the wild-type viral CMV promoter, a methylation-resistant mutant of the CMV promoter, or the mammalian EF-1α promoter. To further enhance expression, endogenous mammalian introns or viral long terminal repeats (LTRs) were included, along with β-globin insulators placed at both the 5′ and 3′ ends of the cassette. Additionally, the constructs were equipped with two antibiotic resistance genes, Hygromycin and G418, and a full-length Woodchuck Hepatitis Virus Posttranscriptional Regulatory Element (WPRE), known to improve mRNA stability and enhance protein expression by modulating RNA structure <span><span>[1]</span></span>.The results indicated that transduction of HEK293–6E cells achieved higher efficiency, resulted in more clones with a stably integrated expression cassette, and stronger protein expression than transduction of Vero cells. The inclusion of β-globin insulators significantly enhanced gene expression in HEK293–6E cells, while their effect in Vero cells was less pronounced. These findings highlight the importance of cell line selection and genetic design in optimizing recombinant protein production.</div></div>","PeriodicalId":19190,"journal":{"name":"New biotechnology","volume":"92 ","pages":"Pages 75-78"},"PeriodicalIF":4.9,"publicationDate":"2026-05-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146093607","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Canola meal protein hydrolysate as a sustainable medium for heterotrophic cultivation of Euglena gracilis","authors":"Ifeyomi Olobayotan ,&nbsp;Nirpesh Dhakal ,&nbsp;Bishnu Acharya ,&nbsp;Darren R. Korber","doi":"10.1016/j.nbt.2026.01.008","DOIUrl":"10.1016/j.nbt.2026.01.008","url":null,"abstract":"<div><div><em>Euglena gracilis</em>, a Generally Recognized as Safe (GRAS) microorganism, is of growing biotechnological interest due to its ability to accumulate diverse cellular metabolites under heterotrophic conditions. This study evaluated canola meal protein hydrolysate (CMPH) as a protein-derived organic nitrogen source to partially substitute conventional complex supplements (yeast extract and tryptone) in heterotrophic <em>E. gracilis</em> cultivation. CMPH was produced by enzymatic hydrolysis of canola meal using trypsin (3000 mL⁻¹, pH 8.0, 37 °C, 24 h). The hydrolysate contained 74.28 ± 5.92 % protein (micro-Kjeldahl), and X-ray fluorescence analysis indicated phosphorus (6.8 %), calcium (16.7 %), potassium (1.7 %), and magnesium (1.2 %) as major mineral components. A Box–Behnken design coupled with response surface methodology (BBD-RSM) was applied to optimize growth conditions in CMPH-supplemented medium. Cultures inoculated at 1.0 × 10⁵ cells mL⁻¹ were incubated at 25 °C in the dark for 168 h. RSM identified pH 6.8, 1 g L⁻¹ CMPH, and 120 h as statistically optimal conditions within the tested design space, yielding 9.8 × 10⁵ cells mL⁻¹ in the absence of added glucose, with a specific growth rate of 0.69 ± 0.23 d⁻¹. Higher cell densities were obtained at increased CMPH concentrations and upon glucose supplementation, reaching levels comparable to standard Hutner’s medium. These results indicate that the RSM-derived optimum reflects efficient nitrogen utilization rather than maximal biomass accumulation. Overall, CMPH demonstrates potential as a functional organic nitrogen supplement for heterotrophic <em>E. gracilis</em> cultivation, supporting the valorization of canola meal as a protein-rich agricultural by-product.</div></div>","PeriodicalId":19190,"journal":{"name":"New biotechnology","volume":"92 ","pages":"Pages 89-99"},"PeriodicalIF":4.9,"publicationDate":"2026-05-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146044124","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}