New biotechnology最新文献_第2页

Applicability of a laccase from the eucalypt wood endophytic fungus Hormonema sp. CECT-13092 for advanced bioethanol production 桉树木材内生真菌 Hormonema sp. CECT-13092 的漆酶在高级生物乙醇生产中的适用性。

IF 4.5 2区生物学

New biotechnology Pub Date : 2025-03-07 DOI: 10.1016/j.nbt.2025.03.001

Luisa García-Fuentevilla, María E. Eugenio, Raquel Martín-Sampedro, David Ibarra

{"title":"Applicability of a laccase from the eucalypt wood endophytic fungus Hormonema sp. CECT-13092 for advanced bioethanol production","authors":"Luisa García-Fuentevilla, María E. Eugenio, Raquel Martín-Sampedro, David Ibarra","doi":"10.1016/j.nbt.2025.03.001","DOIUrl":"10.1016/j.nbt.2025.03.001","url":null,"abstract":"<div><div>This work studies, for the first time, the potential of a laccase from the endophyte fungus <em>Hormonema</em> sp. CECT-13092, compared to a laccase from the saprophyte fungus <em>Trametes villosa</em>, for delignification and detoxification of steam-exploded eucalypt to improve subsequent bioethanol production. Regarding laccase delignification, the use of <em>Hormonema</em> sp. and <em>T. villosa</em> laccases did not show evidence of delignification of steam-exploded material, and rather low glucose and xylose recoveries were obtained during saccharification assays of laccase-treated samples compared with their respective controls. With regard to laccase detoxification, the reduction of the total phenolic inhibitors content presents in steam-exploded material by both laccases (phenols removal of 47 % and 60 % by <em>Hormonema</em> sp. and <em>T. villosa</em> laccases<em>,</em> respectively), triggered the fermentation by <em>Saccharomyces cerevisiae</em> of laccase-treated samples when 0.2 g L<sup>−1</sup> of inoculum was used during a simultaneous saccharification and fermentation (SSF) process. Moreover, when the inoculum was increased from 0.2 to 1.0 g L<sup>−1</sup>, both laccases shortened the yeast lag phase during the SSF process. Then, faster glucose consumption and ethanol production rates (ethanol productivity values of 0.25 and 0.28 g L<sup>−1</sup> h<sup>−1</sup> for <em>T. villosa</em> and <em>Hormonema</em> sp. laccases, respectively, compared to 0.02 g L<sup>−1</sup> h<sup>−1</sup> for control samples) were noticed. This fact proves the high potential of this new entophytic fungal laccase for bioethanol production enhancement, comparable to commercial laccases.</div></div>","PeriodicalId":19190,"journal":{"name":"New biotechnology","volume":"87 ","pages":"Pages 60-71"},"PeriodicalIF":4.5,"publicationDate":"2025-03-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143586427","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Utilization of lactose and whey permeate for the sustainable production of polyhydroxyalkanoates by Hydrogenophaga pseudoflava DSM1034

IF 4.5 2区生物学

New biotechnology Pub Date : 2025-03-03 DOI: 10.1016/j.nbt.2025.02.009

Lorenzo Favaro , Sergio Casella , Elettra Parro , Giuliana Franzosi , Valentina Rodighiero , Marina Basaglia

{"title":"Utilization of lactose and whey permeate for the sustainable production of polyhydroxyalkanoates by Hydrogenophaga pseudoflava DSM1034","authors":"Lorenzo Favaro , Sergio Casella , Elettra Parro , Giuliana Franzosi , Valentina Rodighiero , Marina Basaglia","doi":"10.1016/j.nbt.2025.02.009","DOIUrl":"10.1016/j.nbt.2025.02.009","url":null,"abstract":"<div><div>The utilization of agri-food wastes holds significant importance from both environmental and economic standpoints. Whey permeate, a by-product of cheese manufacturing with high lactose content, could be considered a promising substrate for microbial growth to yield value-added products. Whey permeate was therefore investigated as a potential feedstock for the production of polyhydroxyalkanoates (PHAs) by <em>Hydrogenophaga pseudoflava</em> DSM1034. Although the ability of this microorganism to grow and accumulate PHAs on whey permeate has been previously demonstrated, the PHAs yields were notably low. Therefore, a meticulous inoculum optimization was performed, taking into consideration adaptation to lactose-containing substrates, initial bacterial concentration, and growth kinetics. In media containing lactose as a carbon source, the lag phase, exceeding 40 hours in non-optimized conditions, decreased to only 4 hours. In whey permeate, a cell dry weight (CDW) of 5.49 ± 0.08 g/L was obtained. PHAs fraction in CDW and PHAs yield were 52.48 ± 3.14 % of CDW and 0.144 g/g of consumed sugar, respectively. Furthermore, these results were achieved in flask without hydrolysis of lactose contained in the permeate. Moreover, no galactose remained unutilized in the spent broth demonstrating that <em>H. pseudoflava</em> DSM1034 metabolized both sugars contained in lactose. Additionally, in culture media containing mixtures of glucose and galactose, microbial growth was lower compared to growth on comparable amounts of lactose. This study underscores the potential of whey permeate as a valuable resource for the production of PHAs, offering a sustainable solution for the utilization of dairy industry by-products, mitigating its environmental impact and promoting sustainable resource utilization.</div></div>","PeriodicalId":19190,"journal":{"name":"New biotechnology","volume":"87 ","pages":"Pages 72-81"},"PeriodicalIF":4.5,"publicationDate":"2025-03-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143567767","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

From the lab to the field and closer to the market: Production of the biopolymer cyanophycin in plants

IF 4.5 2区生物学

New biotechnology Pub Date : 2025-02-28 DOI: 10.1016/j.nbt.2025.02.006

Jana Huckauf , Ursula Weisenfeld , Inge Broer

{"title":"From the lab to the field and closer to the market: Production of the biopolymer cyanophycin in plants","authors":"Jana Huckauf , Ursula Weisenfeld , Inge Broer","doi":"10.1016/j.nbt.2025.02.006","DOIUrl":"10.1016/j.nbt.2025.02.006","url":null,"abstract":"<div><div>A range of studies has investigated the production of biopolymers in plants but a comprehensive assessment of feasibility and environmental safety and consumer acceptance is lacking. This review delivers such an assessment. It describes the establishment of the production in tobacco and potato, the analysis of lead events in the greenhouse and in the field, the establishment and upscaling of effective isolation processes and storage conditions, taking the cyanobacterial storage peptide cyanophycin (CGP) as an example. The paper lists several industrial and medical applications of CGP and its building blocks Arg-Asp-dipeptides. This production is especially interesting because the CGP content can exceed 10 % of the dry weight (dw) in the greenhouse and still deliver 4 g per plant in the field. Furthermore, risk assessment of CGP production in potatoes <em>in vitro</em>, <em>in vivo</em>, in the greenhouse, and in the field showed no relevant differences concerning environment or consumer safety compared with the near isogenic control. A consumer choice analysis in four European countries showed a preference for biodegradable CGP in food-wrapping materials over conventional plastic wrapping. Although data on economic feasibility is lacking, CGP as a renewable, biodegradable and CO<sub>2</sub>-neutrally produced compound, is preferable over fossil fuels in many applications.</div></div>","PeriodicalId":19190,"journal":{"name":"New biotechnology","volume":"87 ","pages":"Pages 29-38"},"PeriodicalIF":4.5,"publicationDate":"2025-02-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143520650","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Microalgal biorefineries in sustainable biofuel production and other high-value products

IF 4.5 2区生物学

New biotechnology Pub Date : 2025-02-27 DOI: 10.1016/j.nbt.2025.02.007

Swapnamoy Dutta , Sampriti Kataki , Ishita Banerjee , Cheryl Bernice Pohrmen , Krishna Kumar Jaiswal , Amit K. Jaiswal

{"title":"Microalgal biorefineries in sustainable biofuel production and other high-value products","authors":"Swapnamoy Dutta , Sampriti Kataki , Ishita Banerjee , Cheryl Bernice Pohrmen , Krishna Kumar Jaiswal , Amit K. Jaiswal","doi":"10.1016/j.nbt.2025.02.007","DOIUrl":"10.1016/j.nbt.2025.02.007","url":null,"abstract":"<div><div>Microalgae has been emerging as a promising solution against the backdrop of the global need for sustainable, eco-friendly alternatives. This review article analyses the use of photosynthetic microalgae as an important resource for sustainable biofuel and high value bioproduct production, emphasizing the potential of self-sustaining microalgae biorefineries. A closed-loop, integrated multi-product producing microalgal biorefinery approach could significantly reduce the indicated negative environmental and energy impact from standalone microalgal biofuel generation. The economic feasibility of these biorefineries is linked to their recovery rate, improved by integrating various unit operations as well as multiple product dimensions under optimal conditions, enhancing resource recovery, process efficiency, and profitability. This approach ensures profitability and ubiquitous implementation of microalgal biorefineries, offering a sustainable solution to market demands. In conclusion, making microalgae biorefineries a major player in sustainable bioeconomy underscores the necessity of interdisciplinary research to surmount current challenges and completely realize their advantages.</div></div>","PeriodicalId":19190,"journal":{"name":"New biotechnology","volume":"87 ","pages":"Pages 39-59"},"PeriodicalIF":4.5,"publicationDate":"2025-02-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143537474","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Microfluidic method for rapidly determining the protein and lipid yield of microalgae

IF 4.5 2区生物学

New biotechnology Pub Date : 2025-02-21 DOI: 10.1016/j.nbt.2025.02.001

Shubhanvit Mishra , Ting-Yu Pan , Yi-Ju Liu , Chi-Shuo Chen , Da-Jeng Yao

{"title":"Microfluidic method for rapidly determining the protein and lipid yield of microalgae","authors":"Shubhanvit Mishra , Ting-Yu Pan , Yi-Ju Liu , Chi-Shuo Chen , Da-Jeng Yao","doi":"10.1016/j.nbt.2025.02.001","DOIUrl":"10.1016/j.nbt.2025.02.001","url":null,"abstract":"<div><div>Microalgae are a promising source of green energy. They produce valuable bioproducts, such as proteins and lipids, and remove atmospheric carbon. In this study, we developed a microfluidic chip for culturing and screening for microalgae with high protein and lipid contents. Over 30 microalgae cultures can be grown in parallel in hanging drops on the chip and separately subjected to various experimental treatments, such as random mutations or different light levels. The microfluidic chip has a simple design and was fabricated from optically transparent polymethyl methacrylate; it could be easily operated without complex equipment (e.g., a syringe pump). The design was experimentally validated by culturing Cyanidium sp. and performing in-situ fluorescein measurements of its protein content after various ultraviolet and illumination treatments; significant increases in protein yield were observed for selected mutants (approximately 36 %) and further increased with optimized illumination (2500 lux; approximately 35 %). Moreover, Botryococcus braunii cultured in hanging drops that were separately immobilized using a biocompatible agarose gel for in-situ Raman spectroscopy measurements that rapidly determined the lipid composition. Our developed single-drop Raman spectroscopy method not only quantified the lipid content but also revealed its detailed chemical composition. In summary, the designed microfluidic chip is suitable for in-situ fluorescent assays and Raman microspectroscopy and is a compelling solution for high-throughput screening of algal lipids and proteins, reducing the labor required for breeding mutant algae and optimizing algal cultivation.</div></div>","PeriodicalId":19190,"journal":{"name":"New biotechnology","volume":"87 ","pages":"Pages 20-28"},"PeriodicalIF":4.5,"publicationDate":"2025-02-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143482671","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Biosynthesis of biodegradable plastic, poly(3HB-co-3HP), using methane as sole carbon source in metabolically engineered type II methanotroph, Methylosinus trichosporium OB3b

IF 4.5 2区生物学

New biotechnology Pub Date : 2025-02-19 DOI: 10.1016/j.nbt.2025.02.004

Diep Thi Ngoc Nguyen , Ok Kyung Lee , Jeong-Geol Na , Eun Yeol Lee

{"title":"Biosynthesis of biodegradable plastic, poly(3HB-co-3HP), using methane as sole carbon source in metabolically engineered type II methanotroph, Methylosinus trichosporium OB3b","authors":"Diep Thi Ngoc Nguyen , Ok Kyung Lee , Jeong-Geol Na , Eun Yeol Lee","doi":"10.1016/j.nbt.2025.02.004","DOIUrl":"10.1016/j.nbt.2025.02.004","url":null,"abstract":"<div><div>We report a biosynthetic method for producing poly(3-hydroxybutyrate-co-3-hydroxypropionate) [P(3HB-co-3HP)] copolymer from methane alone; we developed a 3-hydroxypropionate (3HP) biosynthetic pathway starting from methane in <em>Methylosinus trichosporium</em> OB3b and overexpressed 3HP-CoA transferase to optimize 3HP incorporation into P(3HB-co-3HP) copolymers. Upon comparing the β-alanine and malonyl-CoA pathways, we discovered that the latter showed greater potential for the formation of 3HP monomers. In addition, we examined the activities of 3HP-CoA synthetases from three distinct sources and found that the enzyme from <em>Metallosphaera sedula</em> was the most effective for 3HP incorporation. Using methane as the sole carbon source in a shake-flask culture, we developed recombinant strains and found that the best strain was OB3b-MCRM-3S, which formed a P(3HB-co-3HP) copolymer, up to 25.62 % of the biomass, with a maximum 3HP level of 9.02 mol%. Our research demonstrates the successful production of a biopolymer, the P(3HB-co-3HP) copolymer, via methane bioconversion using methanotrophs.</div></div>","PeriodicalId":19190,"journal":{"name":"New biotechnology","volume":"87 ","pages":"Pages 12-19"},"PeriodicalIF":4.5,"publicationDate":"2025-02-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143472612","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Antibacterial and biodegradable PLA-based nanofibers loaded with natural phenolic monoterpenes for sustainable biomedical or food application

IF 4.5 2区生物学

New biotechnology Pub Date : 2025-02-18 DOI: 10.1016/j.nbt.2025.02.005

Lucie Bartošová , Magda Janalíková , Jana Sedlaříková , Alena Opálková Šišková , Katarína Kozics , Michaela Blažíčková , Lucie Matošková , Marek Koutný , Pavel Pleva

{"title":"Antibacterial and biodegradable PLA-based nanofibers loaded with natural phenolic monoterpenes for sustainable biomedical or food application","authors":"Lucie Bartošová , Magda Janalíková , Jana Sedlaříková , Alena Opálková Šišková , Katarína Kozics , Michaela Blažíčková , Lucie Matošková , Marek Koutný , Pavel Pleva","doi":"10.1016/j.nbt.2025.02.005","DOIUrl":"10.1016/j.nbt.2025.02.005","url":null,"abstract":"<div><div>Antibacterial biodegradable PLA-based nanofibers loaded with phenolic monoterpenes - thymol, eugenol, carvacrol, and cinnamaldehyde, were prepared by electrospinning. The effect of bioactive molecule on the surface, thermal, morphological, and biological properties has been investigated about the potential pharmaceutical and food processing applications. Fiber diameters ranged from 320 nm for PLA fibrous mat up to 480 nm for PLA membrane with 6 % thymol. All the prepared active nanofibers exhibited hydrophobic surfaces with a slightly decreasing contact angle after the incorporation of phenols. Antimicrobial testing proved a strong efficiency against <em>Escherichia coli</em> and <em>Staphylococcus aureus</em>, depending on the specific type and content of the bioactive compound. A significant biofilm formation reduction of bioactive PLA nanofibers was revealed against tested microorganisms. Modification of PLA fibers with active molecules did not significantly affect the biodegradation kinetics in comparison to PLA samples with their absence. This study demonstrates the high potential of newly developed PLA-based/phenol nanofibrous membranes for use as antibacterial and antifouling systems applicable in wound dressings and food packaging.</div></div>","PeriodicalId":19190,"journal":{"name":"New biotechnology","volume":"87 ","pages":"Pages 1-11"},"PeriodicalIF":4.5,"publicationDate":"2025-02-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143468737","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Genomic analysis and process optimization for nigericin production in a newly-isolated antimicrobial Streptomyces

IF 4.5 2区生物学

New biotechnology Pub Date : 2025-02-17 DOI: 10.1016/j.nbt.2025.02.002

Kwangjin Jang , Heung-Soon Park , Yeong-Chan Kim , Si-Sun Choi , Youngbin Baek , Eung-Soo Kim

{"title":"Genomic analysis and process optimization for nigericin production in a newly-isolated antimicrobial Streptomyces","authors":"Kwangjin Jang , Heung-Soon Park , Yeong-Chan Kim , Si-Sun Choi , Youngbin Baek , Eung-Soo Kim","doi":"10.1016/j.nbt.2025.02.002","DOIUrl":"10.1016/j.nbt.2025.02.002","url":null,"abstract":"<div><div><em>Streptomyces</em>, the dominant species of actinomycetes, contribute to a large proportion of bioactive natural products. In our previous study, over 2400 culture extracts were subjected to bioassays against <em>Candida albicans</em> and <em>Staphylococcus aureus</em>. Among these, <em>Streptomyces javensis</em> named Inha503, was found to produce nigericin, which exhibits antimicrobial activity against gram-positive bacteria, such as <em>Micrococcus luteus</em> and <em>S. aureus</em>. This study aimed to understand the genomic features of <em>S. javensis</em> Inha503 and optimize the downstream process to improve the titer of nigericin derivatives. Identification of the nigericin biosynthetic gene cluster (BGC) in the chromosome was confirmed using whole-genome bioinformatics and core BGC knockout results. The cultivation time was optimized to 10 days, and nigericin derivatives were identified and quantified using LC/MS. Among the seven-production media for <em>Streptomyces</em>, the highest production of nigericin was obtained in the R5 medium, which exhibited a 1.75-fold increase compared to the previous conditions. Additionally, the nigericin extraction process was optimized using ethyl acetate as a single solvent and a mixture of ethyl acetate and ethanol in a 7:3 ratio. The highest titer of nigericin derivatives were obtained in R5 medium after 10 days with extraction using a mixture of ethyl acetate and ethanol, implying that optimized purification processes with genetic insights of <em>S. javensis</em> Inha503 as a promising platform for bioactive compound production such as nigericin.</div></div>","PeriodicalId":19190,"journal":{"name":"New biotechnology","volume":"86 ","pages":"Pages 97-106"},"PeriodicalIF":4.5,"publicationDate":"2025-02-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143454305","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Efficient surface display of single-chain variable fragments against tumor necrosis factor α on engineered probiotic Saccharomyces boulardii and its application in alleviating intestinal inflammation in vivo

IF 4.5 2区生物学

New biotechnology Pub Date : 2025-02-15 DOI: 10.1016/j.nbt.2025.02.003

Kanghee Han , Ji Sun Park , Young-Woo Kim , Woohyuk Lee , Kyeongsoon Park , Sun-Ki Kim

{"title":"Efficient surface display of single-chain variable fragments against tumor necrosis factor α on engineered probiotic Saccharomyces boulardii and its application in alleviating intestinal inflammation in vivo","authors":"Kanghee Han , Ji Sun Park , Young-Woo Kim , Woohyuk Lee , Kyeongsoon Park , Sun-Ki Kim","doi":"10.1016/j.nbt.2025.02.003","DOIUrl":"10.1016/j.nbt.2025.02.003","url":null,"abstract":"<div><div><em>Saccharomyces boulardii</em> is a probiotic that can alleviate inflammation in the gut. In this study, a novel surface display system was developed by employing two different single-chain variable fragments (scFvs) against human tumor necrosis factor α as model anti-inflammatory proteins. We first optimized the expression levels of the scFvs by selecting strong constitutive promoters, of which expression cassettes were integrated into the <em>S. boulardii</em> chromosome using the CRISPR/Cas9-based genome editing system. As the signal peptide and anchoring motif are key factors affecting the display efficiency of target proteins, we next sought to find their optimal combination for the development of an efficient surface display system in <em>S. boulardii</em>. Among various combinations of signal peptide and anchoring motif, the engineered <em>S. boulardii</em>, which displayed scFvs using the SED1 signal peptide and DAN4 glycophosphatidylinositol domain (derived from <em>Saccharomyces cerevisiae</em>) as the signal peptide and anchoring motif, respectively, exhibited the highest display efficiency. Finally, the anti-inflammatory effects of the engineered <em>S. boulardii</em> strains displaying a high yield of scFvs, including a low disease activity index score, prevention of colon shortening, and reduction in pro-inflammatory cytokines, were validated using a colitis mouse model. Therefore, we believe that our approach has potential applications in the development of engineered <em>S. boulardii</em> displaying other valuable proteins.</div></div>","PeriodicalId":19190,"journal":{"name":"New biotechnology","volume":"86 ","pages":"Pages 107-114"},"PeriodicalIF":4.5,"publicationDate":"2025-02-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143441551","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Conceptual design and environmental evaluation of the Biorefinery approach for R-phycoerythrin extraction and purification

IF 4.5 2区生物学

New biotechnology Pub Date : 2025-01-28 DOI: 10.1016/j.nbt.2025.01.010

Ana Arias , Eduardo Entrena-Barbero , Tanel Ilmjärv , Riccardo Paoli , Francesco Romagnoli , Gumersindo Feijoo , Maria Teresa Moreira

{"title":"Conceptual design and environmental evaluation of the Biorefinery approach for R-phycoerythrin extraction and purification","authors":"Ana Arias , Eduardo Entrena-Barbero , Tanel Ilmjärv , Riccardo Paoli , Francesco Romagnoli , Gumersindo Feijoo , Maria Teresa Moreira","doi":"10.1016/j.nbt.2025.01.010","DOIUrl":"10.1016/j.nbt.2025.01.010","url":null,"abstract":"<div><div>Marine algae are considered promising resources both at present and in the near future. Their availability, together with their molecular structure and properties, increases their applicability in various sectors: food and feed, cosmetics, pharmaceuticals and bioenergy. However, the \"bio\" qualification does not always imply a lower impact compared to fossil-based process schemes. Therefore, to verify the suitability of algae-based scenarios from a sustainable and circular perspective, it is necessary to assess their sustainability potential through process modelling (scaling up from laboratory scale to evaluate their potential at commercial level), environmental assessment (using the Life Cycle Assessment (LCA) method) and circularity analysis (by quantifying circularity indicators focusing on recovery, waste management and effective use of resources). In this context, this research report focused on the techno-economic assessment (TEA) and LCA of three alternative scenarios based on the extraction of R-phycoerythrin from offshore harvested macroalgae: water extraction followed by enzymatic digestion (S01), ultrasound-assisted extraction (S02) and water extraction (S03). In addition, the evaluation of environmental, social and circularity indicators and the application of the Greenness Grid methodology were included. According to the results obtained, S01 is the most promising alternative among the three scenarios due to its process productivity, lower environmental impact and potential sustainable scenario score according to the Green Chemistry assessment. Regarding the economic perspective, S03 is the only one that does not reach economic viability. Future studies should focus on improving process efficiency, promoting the use of renewable energy resources and supporting technological progress in emerging extraction processes.</div></div>","PeriodicalId":19190,"journal":{"name":"New biotechnology","volume":"86 ","pages":"Pages 73-86"},"PeriodicalIF":4.5,"publicationDate":"2025-01-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143066951","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0