New biotechnology最新文献_第2页

Utilization and valorization of lignin and lignin-derived compounds by Pseudomonas putida KT2440: A new role for glutathione peroxidase 恶臭假单胞菌KT2440对木质素及其衍生物的利用和增值：谷胱甘肽过氧化物酶的新作用

IF 4.9 2区生物学

New biotechnology Pub Date : 2026-05-25 Epub Date: 2026-01-11 DOI: 10.1016/j.nbt.2026.01.002

Qing Zhou , Annabel Fransen , Paolo Innocenti , Arthur F.J. Ram , Johannes H. de Winde

{"title":"Utilization and valorization of lignin and lignin-derived compounds by Pseudomonas putida KT2440: A new role for glutathione peroxidase","authors":"Qing Zhou , Annabel Fransen , Paolo Innocenti , Arthur F.J. Ram , Johannes H. de Winde","doi":"10.1016/j.nbt.2026.01.002","DOIUrl":"10.1016/j.nbt.2026.01.002","url":null,"abstract":"<div><div>Lignin, a complex natural aromatic polymer, poses significant challenges to its efficient degradation, hindering the utilization of biomass for many industrial applications. Bacterial degradation of lignin may offer a promising solution to this challenge. This project aimed at elucidating the function of secreted oxidative enzymes from <em>Pseudomonas putida</em> involved in degradation and utilization of lignin and lignin-derived compounds. Using CRISPR-Cas9 and CRISPR-Cas3 systems, the putative lignin-degrading versatile peroxidase gene (VP; <em>PP</em>_<em>1686</em>, originally annotated as glutathione peroxidase GPx) and dye-decolorizing peroxidase gene (<em>PP_3248</em>) were individually knocked out from <em>P. putida</em> KT2440. The ∆PP_1686 mutant exhibited impaired growth and utilization of lignin-derived compounds. This correlated with reduced expression of p-hydroxybenzoate hydroxylase <em>pobA</em> and of DNA repair modules, alongside compensatory upregulation of energy and redox supply pathways. This work expands our knowledge on bacterial glutathione peroxidase by presenting a role beyond ROS scavenging. This work revealed the importance of <em>P. putida</em> VP/GPx in maintaining redox balance while supporting lignin-derived aromatic metabolism, offering new targets for future investigation into stress–metabolism crosstalk and lignin valorization strategies.</div></div>","PeriodicalId":19190,"journal":{"name":"New biotechnology","volume":"92 ","pages":"Pages 1-11"},"PeriodicalIF":4.9,"publicationDate":"2026-05-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145950227","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Harnessing design of experiments and automation significantly improves transient protein production in Chinese hamster ovary cells and accelerates drug discovery 利用实验设计和自动化显著提高中国仓鼠卵巢细胞瞬时蛋白的产生和加速药物的发现。

IF 4.9 2区生物学

New biotechnology Pub Date : 2026-05-25 Epub Date: 2026-01-20 DOI: 10.1016/j.nbt.2026.01.010

Jing Ke , Mandy Li , Sultan Yilmaz, Yan Liu, Hang Thi Thu Nguyen, Pamela Zhang, Laurence Fayadat-Dilman, Jeanne E. Baker

{"title":"Harnessing design of experiments and automation significantly improves transient protein production in Chinese hamster ovary cells and accelerates drug discovery","authors":"Jing Ke , Mandy Li , Sultan Yilmaz, Yan Liu, Hang Thi Thu Nguyen, Pamela Zhang, Laurence Fayadat-Dilman, Jeanne E. Baker","doi":"10.1016/j.nbt.2026.01.010","DOIUrl":"10.1016/j.nbt.2026.01.010","url":null,"abstract":"<div><div>Chinese Hamster Ovary (CHO) cells produce the majority of approved therapeutic proteins and are the de facto platform for rapid, milligram-scale production via transient expression during early biologics discovery. However, performance is highly sensitive to transfection composition, feed strategy, and execution details; and vendor protocols often underperform without platform-specific optimization. We integrated Design of Experiments (DOE) with liquid-handling automation to develop a rapid, data-driven optimization workflow for CHO transient expression. Key factors and ranges were defined for an IgG1 expression and then extended to additional modalities, encompassing coding DNA, PEI transfection reagent, Feed A/B and glucose, inert filler DNA, and the DNA-PEI pipetting sequence that governs brief pre-complexing. Using Response Surface Methodology executed on an automated liquid handler, we generated structured datasets and fitted predictive models that accurately captured main effects, interactions, and curvature. The optimized conditions increased average volumetric productivity by approximately twofold, improved harvest viability, and reduced coding DNA input by about 70 % without detectable loss of product quality (SEC main-peak purity) within our tests. The settings transferred across multiple biologics modalities with consistent gains in titer. Automation eliminates variable‑volume manual pipetting in complex layouts, reduced handling errors, enabled precise short‑duration pre‑complexing steps, and accelerated model‑driven refinement. This approach shorten iteration cycles and de-risks early-stage process development for candidate therapeutic proteins.</div></div>","PeriodicalId":19190,"journal":{"name":"New biotechnology","volume":"92 ","pages":"Pages 40-50"},"PeriodicalIF":4.9,"publicationDate":"2026-05-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146019116","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Corrigendum to “More than meets the eye: Microalgal-bacterial association in polyhydroxybutyrate (PHB) accumulating cultures of Desmodesmus communis”[New Biotechnol 91 (2026), 192–200] “不只是满足于眼睛：微藻-细菌在聚羟基丁酸（PHB）积累培养中的联系”的勘误表[新生物技术91 (2026),192-200]

IF 4.9 2区生物学

New biotechnology Pub Date : 2026-05-25 Epub Date: 2026-01-28 DOI: 10.1016/j.nbt.2026.01.013

Martina Franchini , Mara Simonazzi , Federica Costantini , Valentina Papa , Rossella Pistocchi , Stefano Ratti , Chiara Samorì , Laura Pezzolesi

引用次数: 0

Precision glycoform engineering: Combining plant and in vitro systems for tailored biopharmaceutical production 精密糖苷工程：结合植物和体外系统为量身定制的生物制药生产。

IF 4.9 2区生物学

New biotechnology Pub Date : 2026-05-25 Epub Date: 2026-02-03 DOI: 10.1016/j.nbt.2026.01.014

Mijke R. Sweers, Ruud H.P. Wilbers, Arjen Schots, Pieter Nibbering

{"title":"Precision glycoform engineering: Combining plant and in vitro systems for tailored biopharmaceutical production","authors":"Mijke R. Sweers, Ruud H.P. Wilbers, Arjen Schots, Pieter Nibbering","doi":"10.1016/j.nbt.2026.01.014","DOIUrl":"10.1016/j.nbt.2026.01.014","url":null,"abstract":"<div><div>Protein biopharmaceuticals play a key role in providing effective, targeted, and personalized therapies for diverse diseases, while also preventing and mitigating a broad range of infections. N-glycosylation is a key post-translational modification influencing the biological activity of many protein-based therapeutics, yet structure–function relationships of N-glycans remain poorly understood due to challenges in producing homogeneous glycoforms. Current go-to production hosts, mammalian and yeast cells, often yield heterogeneous glycan profiles and require extensive genetic manipulation. Alternative production hosts such as the plant <em>Nicotiana benthamiana</em>, provide more homogeneous glycosylation and flexibility through transient expression, but are limited in the generation of certain glycoforms. <em>In vitro</em> glycoengineering can overcome these limitations but is time consuming and requires expensive resources. In this study, we show that by combining <em>in planta</em> and <em>in vitro</em> glycoengineering strategies, we can quickly produce a wide range of homogeneous glycoforms of pharmaceutical proteins with high mannose, paucimannose, hybrid and complex N-glycan structures. Using <em>N. benthamiana</em> as a transient expression host, we produced two pharmaceutical glycoproteins — the monoclonal antibody rituximab and the helminth vaccine candidate OoASP-1 — and modified them <em>in vitro</em> using <em>Escherichia coli</em> produced glycoenzymes. The combination of these two glycoengineering systems minimizes the amount of time and resources required, while maintaining high glycan homogeneity. This scalable, flexible, and cost-effective platform opens the door to glycan structure–function relationship studies and can support rational design of next-generation biopharmaceuticals.</div></div>","PeriodicalId":19190,"journal":{"name":"New biotechnology","volume":"92 ","pages":"Pages 100-114"},"PeriodicalIF":4.9,"publicationDate":"2026-05-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146125709","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Canola meal protein hydrolysate as a sustainable medium for heterotrophic cultivation of Euglena gracilis 油菜籽粕蛋白水解物在浅叶草芥异养培养中的可持续应用。

IF 4.9 2区生物学

New biotechnology Pub Date : 2026-05-25 Epub Date: 2026-01-22 DOI: 10.1016/j.nbt.2026.01.008

Ifeyomi Olobayotan , Nirpesh Dhakal , Bishnu Acharya , Darren R. Korber

{"title":"Canola meal protein hydrolysate as a sustainable medium for heterotrophic cultivation of Euglena gracilis","authors":"Ifeyomi Olobayotan , Nirpesh Dhakal , Bishnu Acharya , Darren R. Korber","doi":"10.1016/j.nbt.2026.01.008","DOIUrl":"10.1016/j.nbt.2026.01.008","url":null,"abstract":"<div><div><em>Euglena gracilis</em>, a Generally Recognized as Safe (GRAS) microorganism, is of growing biotechnological interest due to its ability to accumulate diverse cellular metabolites under heterotrophic conditions. This study evaluated canola meal protein hydrolysate (CMPH) as a protein-derived organic nitrogen source to partially substitute conventional complex supplements (yeast extract and tryptone) in heterotrophic <em>E. gracilis</em> cultivation. CMPH was produced by enzymatic hydrolysis of canola meal using trypsin (3000 mL<sup>−1</sup>, pH 8.0, 37 °C, 24 h). The hydrolysate contained 74.28 ± 5.92 % protein (micro-Kjeldahl), and X-ray fluorescence analysis indicated phosphorus (6.8 %), calcium (16.7 %), potassium (1.7 %), and magnesium (1.2 %) as major mineral components. A Box–Behnken design coupled with response surface methodology (BBD-RSM) was applied to optimize growth conditions in CMPH-supplemented medium. Cultures inoculated at 1.0 × 10<sup>5</sup> cells mL<sup>−1</sup> were incubated at 25 °C in the dark for 168 h. RSM identified pH 6.8, 1 g L<sup>−1</sup> CMPH, and 120 h as statistically optimal conditions within the tested design space, yielding 9.8 × 10<sup>5</sup> cells mL<sup>−1</sup> in the absence of added glucose, with a specific growth rate of 0.69 ± 0.23 d<sup>−1</sup>. Higher cell densities were obtained at increased CMPH concentrations and upon glucose supplementation, reaching levels comparable to standard Hutner’s medium. These results indicate that the RSM-derived optimum reflects efficient nitrogen utilization rather than maximal biomass accumulation. Overall, CMPH demonstrates potential as a functional organic nitrogen supplement for heterotrophic <em>E. gracilis</em> cultivation, supporting the valorization of canola meal as a protein-rich agricultural by-product.</div></div>","PeriodicalId":19190,"journal":{"name":"New biotechnology","volume":"92 ","pages":"Pages 89-99"},"PeriodicalIF":4.9,"publicationDate":"2026-05-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146044124","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Advanced stable cell line development based on REMBAC (Rapid Efficient Manifold BACulovirus Transduction) 基于REMBAC（快速高效的多种杆状病毒转导）的高级稳定细胞系的开发。

IF 4.9 2区生物学

New biotechnology Pub Date : 2026-05-25 Epub Date: 2026-01-29 DOI: 10.1016/j.nbt.2026.01.011

Sophie Huber, Sandra Diaz Sanchez, Gordana Wozniak-Knopp, Reingard Grabherr, Manuel Reithofer

{"title":"Advanced stable cell line development based on REMBAC (Rapid Efficient Manifold BACulovirus Transduction)","authors":"Sophie Huber, Sandra Diaz Sanchez, Gordana Wozniak-Knopp, Reingard Grabherr, Manuel Reithofer","doi":"10.1016/j.nbt.2026.01.011","DOIUrl":"10.1016/j.nbt.2026.01.011","url":null,"abstract":"<div><div>The BacMam platform is a scalable and efficient gene delivery system for mammalian cells, enabling the production of recombinant proteins or highly complex structures like bionanoparticles. This study represents the first comprehensive investigation to evaluate the performance of the BacMam platform in a common producer cell line HEK293–6E, on stable transgene expression mediated by the REMBAC (rapid efficient manifold baculovirus transduction) cassette. In this study, six transient high expression cassettes were adopted for stable gene expression and evaluated in HEK293–6E and Vero cells. The constructs included either the wild-type viral CMV promoter, a methylation-resistant mutant of the CMV promoter, or the mammalian EF-1α promoter. To further enhance expression, endogenous mammalian introns or viral long terminal repeats (LTRs) were included, along with β-globin insulators placed at both the 5′ and 3′ ends of the cassette. Additionally, the constructs were equipped with two antibiotic resistance genes, Hygromycin and G418, and a full-length Woodchuck Hepatitis Virus Posttranscriptional Regulatory Element (WPRE), known to improve mRNA stability and enhance protein expression by modulating RNA structure <span><span>[1]</span></span>.The results indicated that transduction of HEK293–6E cells achieved higher efficiency, resulted in more clones with a stably integrated expression cassette, and stronger protein expression than transduction of Vero cells. The inclusion of β-globin insulators significantly enhanced gene expression in HEK293–6E cells, while their effect in Vero cells was less pronounced. These findings highlight the importance of cell line selection and genetic design in optimizing recombinant protein production.</div></div>","PeriodicalId":19190,"journal":{"name":"New biotechnology","volume":"92 ","pages":"Pages 75-78"},"PeriodicalIF":4.9,"publicationDate":"2026-05-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146093607","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Innovative Enzyme-based Seed Coatings for Wheat: Impacts on Plant Growth, P and N Uptake, and Rhizosphere Enzyme Profile. 新型酶基小麦种包衣：对植物生长、磷氮吸收和根际酶谱的影响

IF 4.9 2区生物学

New biotechnology Pub Date : 2026-05-07 DOI: 10.1016/j.nbt.2026.05.003

Natividad Ortega, María C Pilar-Izquierdo, Rodrigo Álvarez-Domingo, Andrea Martín-Pablo, María Medrano, María D Busto

{"title":"Innovative Enzyme-based Seed Coatings for Wheat: Impacts on Plant Growth, P and N Uptake, and Rhizosphere Enzyme Profile.","authors":"Natividad Ortega, María C Pilar-Izquierdo, Rodrigo Álvarez-Domingo, Andrea Martín-Pablo, María Medrano, María D Busto","doi":"10.1016/j.nbt.2026.05.003","DOIUrl":"https://doi.org/10.1016/j.nbt.2026.05.003","url":null,"abstract":"<p><p>Global efforts to achieve sustainable agriculture demand innovative solutions that reduce reliance on chemical fertilizers without compromising crop performance. Enzyme-based seed coatings represent a promising, ecofriendly strategy to enhance nutrient use efficiency and promote early plant vigor in cereals. This study investigated the effects of applying industrial-grade amidohydrolases (A) and phosphatases (Ph) to wheat seeds through pelleting and film-coating techniques. Biodegradable and biosafe materials were used, including zeolite (Z) as filler and methylcellulose (MC), gum arabic, and polyvinyl alcohol (PVA) as binders. The enzyme-based film-coating (F_MC1@Ph/A) increased seedling vigor index (up to 74%) and root length (up to 36%) compared to the uncoated seeds. Shoot length increased (up to 21%) in both pelleted seeds containing zeolite, methylcellulose, and polyvinyl alcohol (P_Z15-MC2-PVA@Ph/A) and film-coated seeds (F_MC1@Ph/A). Additionally, the F_MC1@Ph/A coating increased biomass by up to 47%. Plants from P_Z15-MC2-PVA@Ph/A- and F_MC1@Ph/A-coated seeds showed higher P uptake (50% and 38%, respectively) compared to uncoated seeds, while N uptake remained unaffected except for P_Z15MC2-PVA@Ph/A coating at 14 days after planting. Enzyme-based seed coatings also stimulated rhizosphere enzyme activities during the early stages of growth. Principal component analysis and Pearson correlation studies were conducted across the different seed-coating procedures. Overall, the findings highlight the potential of enzyme-based seed coatings to enhance sustainability in cereal systems. However, as proof of concept performed under controlled conditions, the study presents limitations, and further research is necessary before real-world applications.</p>","PeriodicalId":19190,"journal":{"name":"New biotechnology","volume":" ","pages":""},"PeriodicalIF":4.9,"publicationDate":"2026-05-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147864268","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Adaptive laboratory evolution of Actinobacillus succinogenes for enhanced acid tolerance and succinic acid production. 琥珀酸放线杆菌增强耐酸能力和琥珀酸产量的适应性实验室进化。

IF 4.9 2区生物学

New biotechnology Pub Date : 2026-04-22 DOI: 10.1016/j.nbt.2026.04.002

Min-Seo Park, Minseok Cha, Dong-Seong Kim, Ji-Bong Choi, Young-Lok Cha, Soo-Jung Kim

{"title":"Adaptive laboratory evolution of Actinobacillus succinogenes for enhanced acid tolerance and succinic acid production.","authors":"Min-Seo Park, Minseok Cha, Dong-Seong Kim, Ji-Bong Choi, Young-Lok Cha, Soo-Jung Kim","doi":"10.1016/j.nbt.2026.04.002","DOIUrl":"https://doi.org/10.1016/j.nbt.2026.04.002","url":null,"abstract":"<p><p>The acid tolerance and succinic acid (SA) production in Actinobacillus succinogenes 130Z were improved using adaptive laboratory evolution (ALE). A three-phase continuous culture strategy was employed, gradually decreasing the pH from 6.3 to 5.4. During the ALE process, the medium composition and CO<sub>2</sub> supply were optimized to mitigate acid stress. To relieve acid stress and maintain optimal ionic strength, 0.1M NaCl was supplied, and after maintaining a continuous CO<sub>2</sub> supply during ALE, it was optimized to 2bar and 0.1 vvm. The evolved strain, BAT1, demonstrated superior performance in batch fermentation at pH 5.6, producing 12.8g/L of SA within 48hours, indicating a substantial increase compared to the wild-type strain (4.9g/L). Whole-genome sequencing revealed critical mutations in transport systems (BCCT and NupC/NupG), membrane maintenance proteins (LrgB and acyltransferase), and metabolic regulators (DksA and NAD+ kinase). This study suggests that the efficacy of ALE in developing robust strains for low-pH fermentation and provides potential genetic targets for metabolic engineering of acid tolerance.</p>","PeriodicalId":19190,"journal":{"name":"New biotechnology","volume":" ","pages":""},"PeriodicalIF":4.9,"publicationDate":"2026-04-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147777257","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

More than meets the eye: microalgal-bacterial association in polyhydroxybutyrate (PHB) accumulating cultures of Desmodesmus communis 超过满足眼睛：微藻-细菌联合在聚羟基丁酸（PHB）积累培养的桥丝霉。

IF 4.9 2区生物学

New biotechnology Pub Date : 2026-03-25 Epub Date: 2026-01-08 DOI: 10.1016/j.nbt.2026.01.005

Martina Franchini , Mara Simonazzi , Federica Costantini , Valentina Papa , Rossella Pistocchi , Stefano Ratti , Chiara Samorì , Laura Pezzolesi

{"title":"More than meets the eye: microalgal-bacterial association in polyhydroxybutyrate (PHB) accumulating cultures of Desmodesmus communis","authors":"Martina Franchini , Mara Simonazzi , Federica Costantini , Valentina Papa , Rossella Pistocchi , Stefano Ratti , Chiara Samorì , Laura Pezzolesi","doi":"10.1016/j.nbt.2026.01.005","DOIUrl":"10.1016/j.nbt.2026.01.005","url":null,"abstract":"<div><div>The focus on microalgae within a circular economy framework has been growing due to their ability to utilize inexpensive substrates and produce high-value biomolecules. Among these, the biopolymer polyhydroxybutyrate (PHB) can be accumulated as a storage compound in several microalgal biomasses under nutrient- and light-limiting conditions. Herein, the Chlorophyta <em>Desmodesmus communis</em> was grown under mixotrophic conditions (low light, phosphorus-free medium, 1 g L⁻¹ sodium acetate) in a 1 L semicontinuous system over 25 days. PHB accumulation progressively increased, reaching 57 % w/w on day 25. Since mixotrophic conditions also favor bacterial growth, the associated bacterial community was analyzed through 16S rRNA gene metabarcoding at different time points (day 0, 12, 21, 25) to investigate its potential contribution to PHB accumulation. A selection in bacterial genera was observed after prolonged cultivation. Moreover, antibiotic treatments markedly reduced bacterial diversity and PHB content, indicating a central bacterial role in PHB production and highlighting key genera likely involved. Environmental Scanning Electron Microscopy (ESEM) and Transmission Electron Microscopy (TEM) revealed close interactions between algal and bacterial cells, suggesting a potential endophytic presence of PHB-accumulating bacteria within <em>D. communis</em> cells. These findings propose a new perspective on microalgae-associated bacterial communities, viewing them not as contaminants but as essential contributors to microalgal growth and valuable metabolites production. The results emphasize the importance of considering the bacteria-microalgae consortium in PHB production research, rather than focusing solely on photoautotrophic organisms.</div></div>","PeriodicalId":19190,"journal":{"name":"New biotechnology","volume":"91 ","pages":"Pages 192-200"},"PeriodicalIF":4.9,"publicationDate":"2026-03-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145949060","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Automated optimization of resin selection, wash parameters, and elution parameters for protein A-based purifications 自动优化树脂选择，洗涤参数和洗脱参数的蛋白质为基础的纯化。

IF 4.9 2区生物学

New biotechnology Pub Date : 2026-03-25 Epub Date: 2025-11-11 DOI: 10.1016/j.nbt.2025.11.004

Gracie K. Anderson , Mark Hicks , Penny Hamlyn , L. Andrew Lee , Patrick A. Kates

{"title":"Automated optimization of resin selection, wash parameters, and elution parameters for protein A-based purifications","authors":"Gracie K. Anderson , Mark Hicks , Penny Hamlyn , L. Andrew Lee , Patrick A. Kates","doi":"10.1016/j.nbt.2025.11.004","DOIUrl":"10.1016/j.nbt.2025.11.004","url":null,"abstract":"<div><div>Automated protein purification is essential for meeting the growing demands of high-throughput sample processing capabilities, especially when screening multiple parameters. For instance, protein A-based affinity chromatography is an important downstream antibody purification process that can be automated to maintain high reproducibility with minimized human error. This purification step can clear undesirable host cell proteins (HCP) from Chinese hamster ovary (CHO) cells and capture target monoclonal antibodies, developed for a broad assortment of therapeutic applications, from antibody-expressing cell culture fluid. Selecting optimized parameters for protein A purification is crucial to ensuring productive performance in terms of optimal binding, prevention of undesirable effects from host cell protein, and product aggregate reduction. Herein, we demonstrate a faster and lower cost automated micro-purification workflow to accelerate the screening and selection of resin, wash, and elution parameters for protein A chromatography. In testing three monoclonal antibodies (mAb) and one bispecific monoclonal antibody (bsAb), we observed marked variability in purity and HCP clearance based on the wash buffer and elution pH. We also report that modified intermediate wash buffers containing sodium chloride minimized HCP contamination of eluted samples while preserving high monomer yields. Additionally, we identify differences in performance between resins, including the ability of Praesto Jetted A50 HipH to maintain higher yields at elution pH at which the yields of other resins declined (>pH 4.2). These results highlight the utility of automated microscale purification in reducing time and resource consumption involved in optimizing and executing downstream antibody bioprocessing.</div></div>","PeriodicalId":19190,"journal":{"name":"New biotechnology","volume":"91 ","pages":"Pages 17-26"},"PeriodicalIF":4.9,"publicationDate":"2026-03-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145513561","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0