高纯度d-乳酸野生型乳酸菌的筛选及生物反应器培养

IF 4.9 2区 生物学 Q1 BIOCHEMICAL RESEARCH METHODS
Laura Troiani , Alessia Levante , Hannes Russmayer , Hans Marx , Erasmo Neviani , Valentina Bernini , Camilla Lazzi , Michael Sauer
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引用次数: 0

摘要

可生物降解聚合物是传统油基塑料的绿色替代品,在实现《2030年议程》可持续发展目标方面发挥着关键作用。目前,聚乳酸(PLA)是全球市场上最常见的生物塑料之一。光纯d -乳酸(D-LA)是生产高性能PLA材料的有价值的单体。本研究旨在选择能够生产高光学纯度D-LA的野生型乳酸菌,并确定优化的生物反应器发酵工艺,以最大限度地提高产量。采用酶法和高效液相色谱法对150株来自帕尔马大学(University of Parma)的LAB菌株进行筛选。其中,因D-LA产量高、纯度高(>97 %),选择delbrueckii Lactobacillus UPCCO 2214和Leuconostoc citreum UPCCO 4516进行补料分批培养。发酵参数优化后,L. delbrueckii UPCCO 2214是产D-LA效率最高的菌株,产率为0.74 g/g,体积产率为0.96 g/L/h,优于L. citreum UPCCO 4516。生物反应器的培养有助于了解微生物的生产并提高其潜力。这项工作支持进一步研究和改进D-LA生产工艺,以促进生物材料领域的发展,为工业和环境带来好处。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Screening and bioreactor cultivation of wild-type lactic acid bacteria for high purity D-lactic acid production
Biodegradable polymers are the green alternative to conventional oil-based plastics and have a key role in the achievement of the Sustainable Development Goals of Agenda 2030. Nowadays, polylactic acid (PLA) is one of the most common bioplastics present in the global market. Optically pure D-lactic acid (D-LA) is a valuable monomer for the production of high-performance PLA materials. This study aims to select wild-type lactic acid bacteria capable of producing D-LA with high optical purity and to define an optimized fermentation process in bioreactors to maximize production. A total of 150 LAB strains from the University of Parma Culture Collection (UPCCO) were screened using enzymatic assays and HPLC analysis. Among them, Lactobacillus delbrueckii UPCCO 2214 and Leuconostoc citreum UPCCO 4516 were selected for fed-batch cultivations for their high D-LA production and purity (>97 %). After optimization of fermentation parameters, L. delbrueckii UPCCO 2214 was found to be the most efficient strain for D-LA production, with a yield of 0.74 g/g and a volumetric productivity of 0.96 g/L/h, outperforming L. citreum UPCCO 4516. Bioreactors cultivation has helped to understand microbial production and boost their potentialities. This work supports further investigations and improvements in D-LA production processes to advance the field of biomaterials with benefits for both industry and the environment.
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来源期刊
New biotechnology
New biotechnology 生物-生化研究方法
CiteScore
11.40
自引率
1.90%
发文量
77
审稿时长
1 months
期刊介绍: New Biotechnology is the official journal of the European Federation of Biotechnology (EFB) and is published bimonthly. It covers both the science of biotechnology and its surrounding political, business and financial milieu. The journal publishes peer-reviewed basic research papers, authoritative reviews, feature articles and opinions in all areas of biotechnology. It reflects the full diversity of current biotechnology science, particularly those advances in research and practice that open opportunities for exploitation of knowledge, commercially or otherwise, together with news, discussion and comment on broader issues of general interest and concern. The outlook is fully international. The scope of the journal includes the research, industrial and commercial aspects of biotechnology, in areas such as: Healthcare and Pharmaceuticals; Food and Agriculture; Biofuels; Genetic Engineering and Molecular Biology; Genomics and Synthetic Biology; Nanotechnology; Environment and Biodiversity; Biocatalysis; Bioremediation; Process engineering.
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