Laura Troiani , Alessia Levante , Hannes Russmayer , Hans Marx , Erasmo Neviani , Valentina Bernini , Camilla Lazzi , Michael Sauer
{"title":"高纯度d-乳酸野生型乳酸菌的筛选及生物反应器培养","authors":"Laura Troiani , Alessia Levante , Hannes Russmayer , Hans Marx , Erasmo Neviani , Valentina Bernini , Camilla Lazzi , Michael Sauer","doi":"10.1016/j.nbt.2025.08.005","DOIUrl":null,"url":null,"abstract":"<div><div>Biodegradable polymers are the green alternative to conventional oil-based plastics and have a key role in the achievement of the Sustainable Development Goals of Agenda 2030. Nowadays, polylactic acid (PLA) is one of the most common bioplastics present in the global market. Optically pure D-lactic acid (D-LA) is a valuable monomer for the production of high-performance PLA materials. This study aims to select wild-type lactic acid bacteria capable of producing D-LA with high optical purity and to define an optimized fermentation process in bioreactors to maximize production. A total of 150 LAB strains from the University of Parma Culture Collection (UPCCO) were screened using enzymatic assays and HPLC analysis. Among them, <em>Lactobacillus delbrueckii</em> UPCCO 2214 and <em>Leuconostoc citreum</em> UPCCO 4516 were selected for fed-batch cultivations for their high D-LA production and purity (>97 %). After optimization of fermentation parameters, <em>L. delbrueckii</em> UPCCO 2214 was found to be the most efficient strain for D-LA production, with a yield of 0.74 g/g and a volumetric productivity of 0.96 g/L/h, outperforming <em>L. citreum</em> UPCCO 4516. Bioreactors cultivation has helped to understand microbial production and boost their potentialities. This work supports further investigations and improvements in D-LA production processes to advance the field of biomaterials with benefits for both industry and the environment.</div></div>","PeriodicalId":19190,"journal":{"name":"New biotechnology","volume":"90 ","pages":"Pages 48-55"},"PeriodicalIF":4.9000,"publicationDate":"2025-08-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Screening and bioreactor cultivation of wild-type lactic acid bacteria for high purity D-lactic acid production\",\"authors\":\"Laura Troiani , Alessia Levante , Hannes Russmayer , Hans Marx , Erasmo Neviani , Valentina Bernini , Camilla Lazzi , Michael Sauer\",\"doi\":\"10.1016/j.nbt.2025.08.005\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><div>Biodegradable polymers are the green alternative to conventional oil-based plastics and have a key role in the achievement of the Sustainable Development Goals of Agenda 2030. Nowadays, polylactic acid (PLA) is one of the most common bioplastics present in the global market. Optically pure D-lactic acid (D-LA) is a valuable monomer for the production of high-performance PLA materials. This study aims to select wild-type lactic acid bacteria capable of producing D-LA with high optical purity and to define an optimized fermentation process in bioreactors to maximize production. A total of 150 LAB strains from the University of Parma Culture Collection (UPCCO) were screened using enzymatic assays and HPLC analysis. Among them, <em>Lactobacillus delbrueckii</em> UPCCO 2214 and <em>Leuconostoc citreum</em> UPCCO 4516 were selected for fed-batch cultivations for their high D-LA production and purity (>97 %). After optimization of fermentation parameters, <em>L. delbrueckii</em> UPCCO 2214 was found to be the most efficient strain for D-LA production, with a yield of 0.74 g/g and a volumetric productivity of 0.96 g/L/h, outperforming <em>L. citreum</em> UPCCO 4516. Bioreactors cultivation has helped to understand microbial production and boost their potentialities. This work supports further investigations and improvements in D-LA production processes to advance the field of biomaterials with benefits for both industry and the environment.</div></div>\",\"PeriodicalId\":19190,\"journal\":{\"name\":\"New biotechnology\",\"volume\":\"90 \",\"pages\":\"Pages 48-55\"},\"PeriodicalIF\":4.9000,\"publicationDate\":\"2025-08-20\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"New biotechnology\",\"FirstCategoryId\":\"5\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S1871678425000822\",\"RegionNum\":2,\"RegionCategory\":\"生物学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q1\",\"JCRName\":\"BIOCHEMICAL RESEARCH METHODS\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"New biotechnology","FirstCategoryId":"5","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S1871678425000822","RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"BIOCHEMICAL RESEARCH METHODS","Score":null,"Total":0}
Screening and bioreactor cultivation of wild-type lactic acid bacteria for high purity D-lactic acid production
Biodegradable polymers are the green alternative to conventional oil-based plastics and have a key role in the achievement of the Sustainable Development Goals of Agenda 2030. Nowadays, polylactic acid (PLA) is one of the most common bioplastics present in the global market. Optically pure D-lactic acid (D-LA) is a valuable monomer for the production of high-performance PLA materials. This study aims to select wild-type lactic acid bacteria capable of producing D-LA with high optical purity and to define an optimized fermentation process in bioreactors to maximize production. A total of 150 LAB strains from the University of Parma Culture Collection (UPCCO) were screened using enzymatic assays and HPLC analysis. Among them, Lactobacillus delbrueckii UPCCO 2214 and Leuconostoc citreum UPCCO 4516 were selected for fed-batch cultivations for their high D-LA production and purity (>97 %). After optimization of fermentation parameters, L. delbrueckii UPCCO 2214 was found to be the most efficient strain for D-LA production, with a yield of 0.74 g/g and a volumetric productivity of 0.96 g/L/h, outperforming L. citreum UPCCO 4516. Bioreactors cultivation has helped to understand microbial production and boost their potentialities. This work supports further investigations and improvements in D-LA production processes to advance the field of biomaterials with benefits for both industry and the environment.
期刊介绍:
New Biotechnology is the official journal of the European Federation of Biotechnology (EFB) and is published bimonthly. It covers both the science of biotechnology and its surrounding political, business and financial milieu. The journal publishes peer-reviewed basic research papers, authoritative reviews, feature articles and opinions in all areas of biotechnology. It reflects the full diversity of current biotechnology science, particularly those advances in research and practice that open opportunities for exploitation of knowledge, commercially or otherwise, together with news, discussion and comment on broader issues of general interest and concern. The outlook is fully international.
The scope of the journal includes the research, industrial and commercial aspects of biotechnology, in areas such as: Healthcare and Pharmaceuticals; Food and Agriculture; Biofuels; Genetic Engineering and Molecular Biology; Genomics and Synthetic Biology; Nanotechnology; Environment and Biodiversity; Biocatalysis; Bioremediation; Process engineering.