Effect of culture medium composition and reuse on the growth of Porphyridium cruentum

IF 4.9 2区 生物学 Q1 BIOCHEMICAL RESEARCH METHODS
Florencia Cáceres-Ferroni , María Salinas-García , Silvia Villaró-Cos , Elia Rivera-Sánchez , Tomás Lafarga
{"title":"Effect of culture medium composition and reuse on the growth of Porphyridium cruentum","authors":"Florencia Cáceres-Ferroni ,&nbsp;María Salinas-García ,&nbsp;Silvia Villaró-Cos ,&nbsp;Elia Rivera-Sánchez ,&nbsp;Tomás Lafarga","doi":"10.1016/j.nbt.2025.09.001","DOIUrl":null,"url":null,"abstract":"<div><div>The aim of this study was to develop a fertiliser-based culture medium to reduce production costs and to enhance the sustainability of producing <em>Porphyridium cruentum</em>, a red marine microalga of commercial interest. Additionally, the impact of water recirculation on microalgal growth was assessed. Overall, the results indicated that the nitrogen source significantly affected biomass growth, with sodium nitrate supporting higher biomass productivity (0.23 g·L<sup>−1</sup>·day<sup>−1</sup>), surpassing sodium nitrite (0.18 g·L<sup>−1</sup>·day<sup>−1</sup>) and ammonium chloride (0.14 g·L<sup>−1</sup>·day<sup>−1</sup>). Urea had a negative impact on growth. A N:P molar ratio of 20:1 increased biomass productivity by approximately 24 % compared to the lower ratio studied (8:1) while also reducing phosphorus demand. The optimal medium composition was: 1.75 g·L<sup>−1</sup> NaNO<sub>3</sub>, 0.23 g·L<sup>−1</sup> K<sub>2</sub>HPO<sub>4</sub>·3 H<sub>2</sub>O, 0.04 g·L<sup>−1</sup> CaCl<sub>2</sub>, 0.49 g·L<sup>−1</sup> MgSO<sub>4</sub>·7 H<sub>2</sub>O, and 24.0 mg·L<sup>−1</sup> Karentol®. Lastly, water reutilisation negatively impacted biomass concentration, promoting the accumulation of extracellular organic carbon and bacteria as well as increasing the viscosity and turbidity of the culture.</div></div>","PeriodicalId":19190,"journal":{"name":"New biotechnology","volume":"90 ","pages":"Pages 65-76"},"PeriodicalIF":4.9000,"publicationDate":"2025-09-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"New biotechnology","FirstCategoryId":"5","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S1871678425000834","RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"BIOCHEMICAL RESEARCH METHODS","Score":null,"Total":0}
引用次数: 0

Abstract

The aim of this study was to develop a fertiliser-based culture medium to reduce production costs and to enhance the sustainability of producing Porphyridium cruentum, a red marine microalga of commercial interest. Additionally, the impact of water recirculation on microalgal growth was assessed. Overall, the results indicated that the nitrogen source significantly affected biomass growth, with sodium nitrate supporting higher biomass productivity (0.23 g·L−1·day−1), surpassing sodium nitrite (0.18 g·L−1·day−1) and ammonium chloride (0.14 g·L−1·day−1). Urea had a negative impact on growth. A N:P molar ratio of 20:1 increased biomass productivity by approximately 24 % compared to the lower ratio studied (8:1) while also reducing phosphorus demand. The optimal medium composition was: 1.75 g·L−1 NaNO3, 0.23 g·L−1 K2HPO4·3 H2O, 0.04 g·L−1 CaCl2, 0.49 g·L−1 MgSO4·7 H2O, and 24.0 mg·L−1 Karentol®. Lastly, water reutilisation negatively impacted biomass concentration, promoting the accumulation of extracellular organic carbon and bacteria as well as increasing the viscosity and turbidity of the culture.
培养基组成及重复使用对黄斑紫菜生长的影响
这项研究的目的是开发一种以肥料为基础的培养基,以降低生产成本,并提高生产具有商业价值的红色海洋微藻——cruentum卟啉藻的可持续性。此外,还评估了水循环对微藻生长的影响。总体而言,氮源显著影响生物量生长,硝酸钠支持更高的生物量生产力(0.23 g·L−1·day−1),超过亚硝酸钠(0.18 g·L−1·day−1)和氯化铵(0.14 g·L−1·day−1)。尿素对生长有负面影响。与所研究的较低比例(8:1)相比,氮磷摩尔比为20:1的生物量生产力提高了约24% %,同时也减少了对磷的需求。最佳培养基组成为:1.75 g·L−1 NaNO3、0.23 g·L−1 K2HPO4·3 H2O、0.04 g·L−1 CaCl2、0.49 g·L−1 MgSO4·7 H2O和24.0 mg·L−1 Karentol®。最后,水的再利用对生物量浓度产生了负面影响,促进了细胞外有机碳和细菌的积累,并增加了培养物的粘度和浊度。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
求助全文
约1分钟内获得全文 求助全文
来源期刊
New biotechnology
New biotechnology 生物-生化研究方法
CiteScore
11.40
自引率
1.90%
发文量
77
审稿时长
1 months
期刊介绍: New Biotechnology is the official journal of the European Federation of Biotechnology (EFB) and is published bimonthly. It covers both the science of biotechnology and its surrounding political, business and financial milieu. The journal publishes peer-reviewed basic research papers, authoritative reviews, feature articles and opinions in all areas of biotechnology. It reflects the full diversity of current biotechnology science, particularly those advances in research and practice that open opportunities for exploitation of knowledge, commercially or otherwise, together with news, discussion and comment on broader issues of general interest and concern. The outlook is fully international. The scope of the journal includes the research, industrial and commercial aspects of biotechnology, in areas such as: Healthcare and Pharmaceuticals; Food and Agriculture; Biofuels; Genetic Engineering and Molecular Biology; Genomics and Synthetic Biology; Nanotechnology; Environment and Biodiversity; Biocatalysis; Bioremediation; Process engineering.
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:604180095
Book学术官方微信