New biotechnology最新文献

{"title":"Ruminal microbial responses to Moringa oleifera feed in lactating goats (Capra hircus): A metagenomic exploration","authors":"Chitra Nehra ,&nbsp;Vemula Harshini ,&nbsp;Nitin Shukla ,&nbsp;Priyank Chavda ,&nbsp;Minal Bhure ,&nbsp;Kaksha Savaliya ,&nbsp;Sonal Patil ,&nbsp;Tejas Shah ,&nbsp;Ramesh Pandit ,&nbsp;Niteen V. Patil ,&nbsp;Ashutosh K. Patel ,&nbsp;Subhash Kachhawaha ,&nbsp;Ram N. Kumawat ,&nbsp;Madhvi Joshi ,&nbsp;Chaitanya G. Joshi","doi":"10.1016/j.nbt.2025.01.006","DOIUrl":"10.1016/j.nbt.2025.01.006","url":null,"abstract":"<div><div>The purpose of the current study was to explore the effects of <em>Moringa oleifera</em> feed on the taxonomy and function of the rumen microbial community, and further to evaluate its impact on milk yield and body weight in lactating goats. Nineteen goats were divided into moringa leaf diet (ML; n = 10) and masoor straw (MS; n = 9) groups. For each group fortnight milk yield and body weight was recorded. Rumen solid and liquid fraction samples were processed for metagenomic shotgun sequencing and further analysed. The pairwise comparison between the two groups showed a significant increase (p-value- &lt;0.01) in milk yield of the ML goats after the 4th fortnight interval onwards. The metagenomic analysis revealed <em>Bacteroidetes</em> and <em>Firmicutes</em> are the most abundant phyla, with increased <em>Bacteroidetes</em> in response to the moringa diet. The ML group exhibited a reduction in microbial diversity, with an increase in <em>Prevetolla</em> and <em>Bacteroidales</em> populations which are positively associated with carbohydrate, protein, and VFA metabolism, and an increased proportions of <em>Treponema sp., Ruminococcus sp., Ruminobacter amylophilus,</em> and <em>Aeromonas</em>, indicating improved cellulose and nitrogen metabolism. KEGG analysis revealed significant changes in microbial gene pool and metabolic pathways, particularly in carbohydrate metabolism, propanoate metabolism, and fatty acid synthesis genes. These microbial and functional shifts are correlated with improvements in milk yield, growth rates, and potentially reduced methane emissions.This study highlighted the potential benefits of feeding moringa in the animal production system. However, furthermore experimental evidence including genetic and environmental effects is needed for a comprehensive understanding of moringa feed's impact on goat health and productivity.</div></div>","PeriodicalId":19190,"journal":{"name":"New biotechnology","volume":"86 ","pages":"Pages 87-96"},"PeriodicalIF":4.5,"publicationDate":"2025-01-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143047376","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Screening a 681-membered yeast collection for the secretion of proteins with antifungal activity","authors":"Alicia Maciá Valero ,&nbsp;Fatemehalsadat Tabatabaeifar ,&nbsp;Sonja Billerbeck","doi":"10.1016/j.nbt.2025.01.008","DOIUrl":"10.1016/j.nbt.2025.01.008","url":null,"abstract":"<div><div>Fungal pathogens pose a threat to human health and food security. Few antifungals are available and resistance to all has been reported. Novel strategies to control plant and human pathogens as well as food spoilers are urgently required. Environmental yeasts provide a functionally diverse, yet underexploited potential for fungal control based on their natural competition via the secretion of proteins and other small molecules such as iron chelators, volatile organic compounds or biosurfactants. However, there is a lack of standardized workflows to systematically access application-relevant yeast-based compounds and understand their molecular functioning. Towards this goal, we developed a workflow to identify and characterize yeast isolates that are active against spoilage yeasts and relevant human and plant pathogens, herein focusing on discovering yeasts that secrete antifungal proteins. The workflow includes the classification of the secreted molecules and cross-comparison of their antifungal capacity using an independent synthetic calibrant. Our workflow delivered a collection of 681 yeasts of which 212 isolates (31 %) displayed antagonism against at least one target strain. While 57.5 % of the active yeasts showed iron-depended antagonism, likely due to pulcherrimin-like iron chelators, 31.7 % secreted antifungal proteins. Those yeast candidates clustered within twelve OTUs, showed narrow and broad target spectra, and several showed a broad pH and temperature activity profile. Given the tools for yeast biotechnology and protein engineering available, our collection can serve as a rich starting point for genetic and molecular characterization of the various antifungal phenotypes, their mode of action and their future exploitation.</div></div>","PeriodicalId":19190,"journal":{"name":"New biotechnology","volume":"86 ","pages":"Pages 55-72"},"PeriodicalIF":4.5,"publicationDate":"2025-01-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143059649","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Optimization of genome editing by CRISPR ribonucleoprotein for high efficiency of germline transmission of Sox9 in zebrafish","authors":"Kangning Yang ,&nbsp;Le Cai ,&nbsp;Yu Zhao,&nbsp;Hanhua Cheng,&nbsp;Rongjia Zhou","doi":"10.1016/j.nbt.2025.01.009","DOIUrl":"10.1016/j.nbt.2025.01.009","url":null,"abstract":"<div><div>Primordial germ cells (PGCs) are the first germline stem cells to emerge during early embryonic development and are essential for the propagation and survival of species. Genome editing creates mutagenesis possibilities in vivo, but the generation of precise mutations in PGCs is still challenging. Here, we report an optimized approach for highly efficient genome editing via introducing biallelic variations in early embryos in zebrafish. We adopted an extended, GC-rich, and chemically modified sgRNA along with microinjection of the CRISPR ribonucleoprotein (RNP) complex into the yolk sac at the 1-cell stage. We found that genome editing of <em>Sox9a</em> generated a high proportion of heterozygotes with edited alleles in the F₁ generation, indicating biallelic editing. Deep sequencing and mapping the edited cells from early embryos to future tissues revealed that the edited founder has a dominantly edited allele, <em>sox9a</em> M1, accounting for over 99 % of alleles in the testis. Specifically, all offspring of the founder inherited the edited allele, suggesting nearly complete editing of the alleles in early germline cells. Overall, the optimization delineates biallelic editing of <em>sox9a</em> in early embryos and transmission of edited alleles to offspring, thus presenting a method to create a desired genetic mutation line of <em>Sox9a</em> avoiding lengthy traditional crossbreeding.</div></div>","PeriodicalId":19190,"journal":{"name":"New biotechnology","volume":"86 ","pages":"Pages 47-54"},"PeriodicalIF":4.5,"publicationDate":"2025-01-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143029209","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Utilization of acid whey and oat pomace in succinic acid fermentation","authors":"Corina Kleps ,&nbsp;Ralf Malchow ,&nbsp;Judith Ettinger ,&nbsp;Julia Dalichow ,&nbsp;Roland Schneider ,&nbsp;Joachim Venus ,&nbsp;Daniel Pleissner","doi":"10.1016/j.nbt.2025.01.007","DOIUrl":"10.1016/j.nbt.2025.01.007","url":null,"abstract":"<div><h3>Aim</h3><div>of this study was to investigate the by-products acid whey and oat pomace as nutrient sources for succinic acid production by <em>Actinobacillus succinogenes</em>. Both by-products provide carbon sources in form of glucose and/or lactose without any pre-treatment. Yields of succinic acid per g total sugars consumed after 24 h were between 0.6 and 0.7 in control medium, acid whey, and in acid whey/oat pomace mixtures. A yield of more than 0.8 g per g was found in oat pomace after 24 h, which further increased to 1.0 g per g after 48 h. For the fermentation carried out with acid whey and oat pomace mixed at a ratio of 1:1 a productivity of 0.52 g L⁻¹ h⁻¹ was obtained. The productivities in control medium, acid whey, oat pomace, acid whey/oat pomace (2:1), and acid whey/oat pomace (3:1) were 16 %, 75 %, 48 %, 46 %, and 48 % less, respectively, indicating the necessity of finding the right balance of nutrients. The results of this study contribute to the decentralized utilization of food residues and even, despite the high value of succinic acid as platform chemical, to a recirculation into new food products.</div></div>","PeriodicalId":19190,"journal":{"name":"New biotechnology","volume":"86 ","pages":"Pages 31-38"},"PeriodicalIF":4.5,"publicationDate":"2025-01-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143008790","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Machine learning reveals novel compound for the improved production of chitooligosaccharides in Escherichia coli","authors":"Friederike Mey ,&nbsp;Gaetan De Waele ,&nbsp;Wouter Demeester ,&nbsp;Chiara Guidi ,&nbsp;Dries Duchi ,&nbsp;Tomek Diederen ,&nbsp;Hanne Kochuyt ,&nbsp;Kirsten Van Huffel ,&nbsp;Nicola Zamboni ,&nbsp;Willem Waegeman ,&nbsp;Marjan De Mey","doi":"10.1016/j.nbt.2025.01.005","DOIUrl":"10.1016/j.nbt.2025.01.005","url":null,"abstract":"<div><div>In order to improve predictability of outcome and reduce costly rounds of trial-and-error, machine learning models have been of increasing importance in the field of synthetic biology. Besides applications in predicting genome annotation, process parameters and transcription initiation frequency, such models have also been of help for pathway optimization. The latter is a common strategy in metabolic engineering and improves the production of a desirable compound by optimizing enzyme expression levels of the production pathway. However, engineering steps might not lead to sufficient improvement, and bottlenecks may remain hidden among the hundreds of metabolic reactions occurring in a living cell, especially if the production pathway is highly interconnected with other parts of the cell’s metabolism. Here, we use the synthesis of chitooligosaccharides (COS) to show that the production from such complex pathways can be improved by using machine learning models and feature importance analysis to find new compounds with an impact on COS production. We screened <em>Escherichia coli</em> libraries of engineered transcription regulators with an expected broad range of metabolic diversity and trained several machine learning models to predict COS production titers. Subsequent feature analysis led to the finding of iron, whose addition we could show improved COS production in vivo up to two-fold. Additionally, the analysis revealed important clues for future engineering steps.</div></div>","PeriodicalId":19190,"journal":{"name":"New biotechnology","volume":"86 ","pages":"Pages 39-46"},"PeriodicalIF":4.5,"publicationDate":"2025-01-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143008505","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Increasing the sustainability of photoautotrophic microalgae production by minimising freshwater requirements","authors":"Elia Rivera-Sánchez ,&nbsp;Silvia Villaró-Cos ,&nbsp;María Salinas-García ,&nbsp;Tomás Lafarga","doi":"10.1016/j.nbt.2025.01.004","DOIUrl":"10.1016/j.nbt.2025.01.004","url":null,"abstract":"<div><div>There are now several companies that are producing microalgae such as <em>Arthrospira platensis</em>, <em>Chlorella vulgaris,</em> and <em>Dunaliella salina</em>, among others. They are cultivated mainly in large-scale raceway and tubular photobioreactors. Microalgae production represents a sustainable alternative to conventional biomass production. Microalgae can be used to manufacture agricultural products, animal feed, food and other commercial products. The water requirements for cultivating microalgae are significant, exceeding 1 m³·kg⁻¹. This value varies depending on the production strategy. One of the main reasons for water loss is evaporation, which is influenced by the photobioreactor location, the season, and the operating conditions. Efforts are being made to reduce water requirements and make microalgae production economically viable and more environmentally friendly. Several strategies are being investigated for reducing freshwater use in microalgae cultivation; these include reusing the culture medium and producing microalgae using seawater or wastewater. Such strategies not only reduce water consumption, but also reduce nutrient consumption and costs while increasing sustainability.</div></div>","PeriodicalId":19190,"journal":{"name":"New biotechnology","volume":"86 ","pages":"Pages 14-24"},"PeriodicalIF":4.5,"publicationDate":"2025-01-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143008800","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Advances in the development of microarray patches in biomedicine","authors":"Pooyan Makvandi ,&nbsp;Rezvan Jamaledin ,&nbsp;Mohammad Mofidfar ,&nbsp;Xiangdong Wang ,&nbsp;Tae Seok Moon","doi":"10.1016/j.nbt.2025.01.003","DOIUrl":"10.1016/j.nbt.2025.01.003","url":null,"abstract":"<div><div>Microarray patches (MAPs) have been employed to deliver therapeutic payloads and for detection purposes. Research has been conducted to develop novel designs in material chemistry and the architecture of microarray, which have opened up the possibility for broader applications of MAPs. However, MAPs have yet to be clinically implemented fully. Addressing the current challenges and maximizing opportunities will pave the way to translate the relevant technologies from bench to bedside.</div></div>","PeriodicalId":19190,"journal":{"name":"New biotechnology","volume":"86 ","pages":"Pages 25-30"},"PeriodicalIF":4.5,"publicationDate":"2025-01-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143008794","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Harnessing nature's palette: Exploring photosynthetic pigments for sustainable biotechnology","authors":"Roxana Ángeles ,&nbsp;João Carvalho ,&nbsp;Ingrid Hernández-Martínez ,&nbsp;Marcia Morales-Ibarría ,&nbsp;Joana C. Fradinho ,&nbsp;Maria A.M. Reis ,&nbsp;Raquel Lebrero","doi":"10.1016/j.nbt.2025.01.001","DOIUrl":"10.1016/j.nbt.2025.01.001","url":null,"abstract":"<div><div>Photosynthetic microorganisms such as cyanobacteria, microalgae, and anoxygenic phototrophic bacteria (APB) have emerged as sustainable and economic biotechnology platforms due to their ability to transform energy from light into chemicals through photosynthesis. The light is absorbed by photosynthetic pigment-protein antenna complexes which are composed of pigments such as bacteriochlorophylls (BChl) and carotenoids in APB, and chlorophylls (Chl), phycobiliproteins (PBP), and carotenoids in cyanobacteria and microalgae. These photosynthetic pigments are essential in the physiology of photosynthetic microorganisms and offer significant health benefits due to their potent antioxidant activity, with properties that include anticancer, antiaging, antiproliferative, anti-inflammatory, and neuroprotective effects. This review first provides an overview of current advances in photosynthetic pigment synthesis and the latest strategies to increase pigment content in cyanobacteria, microalgae, and APB. It then delves into the pigment production process, covering biosynthetic pathways, critical environmental parameters, and extraction methods. Finally, the potential marketability of photosynthetic pigments together with current limitations are discussed.</div></div>","PeriodicalId":19190,"journal":{"name":"New biotechnology","volume":"85 ","pages":"Pages 84-102"},"PeriodicalIF":4.5,"publicationDate":"2025-01-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142952263","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The microalgal sector in Europe: Towards a sustainable bioeconomy","authors":"Irene Gallego ,&nbsp;Nikola Medic ,&nbsp;Jakob Skov Pedersen ,&nbsp;Praveen Kumar Ramasamy ,&nbsp;Johan Robbens ,&nbsp;Elke Vereecke ,&nbsp;Jörg Romeis","doi":"10.1016/j.nbt.2025.01.002","DOIUrl":"10.1016/j.nbt.2025.01.002","url":null,"abstract":"<div><div>Microalgae are a diverse group of photosynthetic microorganisms that can be exploited to produce sustainable food and feed products, alleviate environmental pollution, or sequester CO₂ to mitigate climate change, among other uses. To optimize resource use and integrate industrial waste streams, it is essential to consider factors such as the biology and cultivation parameters of the microalgal strains, as well as the cultivation system and processing technologies employed. This paper reviews the main commercial applications of microalgae (including cyanobacteria) and examines the biological and biotechnological aspects critical to the sustainable processing of microalgal biomass and its derived compounds. We also provide an up-to-date overview of the microalgal sector in Europe considering the strain, cultivation system and commercial application. We have identified 146 different microalgal-derived products from 66 European microalgae producers, and 49 additional companies that provide services and technologies, such as optimization and scalability of the microalgal production. The most widely cultivated microalga is ‘spirulina’ (<em>Limnospira</em> spp.), followed by <em>Chlorella</em> spp. and <em>Nannochloropsis</em> spp., mainly for human consumption and cosmetics. The preferred cultivation system in Europe is the photobioreactor. Finally, we discuss the logistic and regulatory challenges of producing microalgae at industrial scale, particularly in the European Union, and explore the potential of new genomic techniques and bioprocessing to foster a sustainable bioeconomy in the microalgal sector.</div></div>","PeriodicalId":19190,"journal":{"name":"New biotechnology","volume":"86 ","pages":"Pages 1-13"},"PeriodicalIF":4.5,"publicationDate":"2025-01-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142952266","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Towards polyethylene terephthalate valorisation into PHB using an engineered Comamonas testosteroni strain","authors":"Francisco J. Molpeceres-García,&nbsp;David Sanz-Mata,&nbsp;Alejandro García-Miro,&nbsp;Alicia Prieto,&nbsp;Jorge Barriuso","doi":"10.1016/j.nbt.2024.12.005","DOIUrl":"10.1016/j.nbt.2024.12.005","url":null,"abstract":"<div><div>The abundant production of plastic materials, coupled with their recalcitrant nature, makes plastic waste a major challenge as a pollutant. Polyethylene terephthalate (PET) is a polyester formed by polycondensation of terephthalic acid (TPA) and ethylene glycol (EG). This plastic polymer can be completely depolymerized to its monomers using microbial enzymes. In this study, we verified <em>in silico</em> and <em>in vivo</em> that the bacterium <em>Comamonas testosteroni</em> RW31 is able to assimilate TPA and to produce the bioplastic polyhydroxybutyrate (PHB). This bacterium was engineered to heterologously express a fusion of the PET-degrading enzymes FAST-PETase and <em>Is</em>MHETase. We verified that our strain successfully secretes the enzymes and depolymerize PET both <em>in vitro</em> and <em>in vivo</em>, achieving a weight loss of 37.1 % and 0.83 %, respectively. We also studied its capacity to form biofilm. Furthermore, our strain can employ bis(2-hydroxyethyl) terephthalate (BHET), an intermediate of PET degradation, as feedstock to accumulate PHB up to 12.03 % of its dry weight in 14 h. Our findings highlight <em>C. testosteroni</em> RW31 as a promising chassis for synthetic biology strategies aimed at upcycling PET waste.</div></div>","PeriodicalId":19190,"journal":{"name":"New biotechnology","volume":"85 ","pages":"Pages 75-83"},"PeriodicalIF":4.5,"publicationDate":"2024-12-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142872484","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}