New biotechnology最新文献

{"title":"Characterization and orthogonality assessment of two quorum sensing systems for synthetic biology applications","authors":"Jasmine De Baets,&nbsp;Brecht De Paepe,&nbsp;Marjan De Mey","doi":"10.1016/j.nbt.2025.08.001","DOIUrl":"10.1016/j.nbt.2025.08.001","url":null,"abstract":"<div><div>Quorum sensing systems have a broad range of applications within the field of synthetic biology. However, a bottleneck is the optimization and tuning of these systems due to the lack of standardization and complete characterization. In this research, two quorum sensing systems, namely the LasI/LasR and the EsaI/EsaR system, were fully characterized in the model host organism <em>Escherichia coli</em>. Furthermore, insight was gained in the interplay between the various parts of these systems. To further expand the range of possibilities with these quorum sensing systems, the orthogonality of the two systems was assessed to allow simultaneous use within the same cell without interfering crosstalk. This assessment was performed on three levels: promoter, signal and synthase crosstalk. It was demonstrated that LasR is able to interact with the promoter of the EsaI/EsaR system, albeit to a low extent. Additionally, LasR was able to respond to the autoinducers produced by EsaI. To solve the promoter crosstalk, a nucleotide change was introduced into the binding site of EsaR within the promoter region. Additionally, LasR mutants were created rationally and screened for decreased response to EsaI while retaining functionality. The best performing mutant, LasR(P117S), was further characterized. In conclusion, we have further unlocked the potential of quorum sensing systems for synthetic biology applications by obtaining two functional, characterized and orthogonal quorum sensing systems.</div></div>","PeriodicalId":19190,"journal":{"name":"New biotechnology","volume":"90 ","pages":"Pages 20-35"},"PeriodicalIF":4.9,"publicationDate":"2025-08-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144886643","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Public perception of new plant breeding techniques for sustainable production of feed and food in the Czech Republic","authors":"Naděžda Čadová ,&nbsp;Jaroslav Doležel ,&nbsp;Ivo Frébort","doi":"10.1016/j.nbt.2025.08.002","DOIUrl":"10.1016/j.nbt.2025.08.002","url":null,"abstract":"<div><div>A survey was conducted among 1676 Czech citizens in the autumn of 2024 to explore their attitudes toward different methods of crop improvement, which are based on the modification of genetic information. The study focused on three techniques: classical mutagenesis, insertion of foreign genetic material, and genome editing. The findings revealed that a significant part of the general public was unfamiliar with these technologies and their legal status. A majority of respondents mistakenly believed that currently permitted mutation breeding using radiation or chemical mutagens is illegal, while conversely assuming that techniques strictly regulated in Europe, such as transgenesis and genome editing, are allowed. Despite using different survey methods and question wording, the study pointed to a modest positive shift in public attitudes toward new genetic technologies compared to earlier surveys. For example, support for legalising genome editing rose from 22 % in 2019 to 47.6 % in 2024, and the proportion of respondents who would buy food containing genetically modified ingredients increased from 35 % to 47.4 %. Acceptance of these methods varied across demographic groups, with younger, more educated, and male respondents showing higher levels of support. While many acknowledge potential benefits such as reduced pesticide use, concerns about safety, ethics, and environmental impact remain. In general, the Czech public demonstrates cautious optimism, underscoring the importance of transparency and clear product labelling.</div></div>","PeriodicalId":19190,"journal":{"name":"New biotechnology","volume":"90 ","pages":"Pages 13-19"},"PeriodicalIF":4.9,"publicationDate":"2025-08-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144829657","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Viability assessment and biomass valorization of recombinant Komagataella phaffii","authors":"Sarah Gangl ,&nbsp;Maik Hilgarth ,&nbsp;Christian Spielhaupter ,&nbsp;Frank Schneider ,&nbsp;Christiane Herrmann ,&nbsp;Christina Recher ,&nbsp;Tobias Baumgartner ,&nbsp;Martina Geier ,&nbsp;Birgit Wassermann ,&nbsp;Anton Glieder","doi":"10.1016/j.nbt.2025.07.005","DOIUrl":"10.1016/j.nbt.2025.07.005","url":null,"abstract":"<div><div><em>Komagataella phaffii</em> has become a key player in biotechnology used for the production of heterologous proteins with numerous GRAS notices and QPS status released in the past years. While most of these descriptions and scientific studies focus on strain engineering, products and cultivation processes, this paper provides fundamental data about the general viability of <em>K. phaffii</em> after post-production biomass treatments and co-incubations with non-sterile soil and water samples. A comparison of different <em>K. phaffii</em> strains uncovered a high thermal sensitivity for all tested strains, which was further enhanced under alkaline conditions. A rapid reduction in the viable cell number started at approx. 52 °C and terminated in a full loss of 10⁹ viable cells per milliliter within seconds at 70 °C. Survival rates of untreated non-GMO and GMO <em>K. phaffii</em> strains in water- and soil-slurry-microcosms demonstrated that the number of viable <em>K. phaffii</em> cells decreased rapidly and comparably to a <em>Saccharomyces cerevisiae</em> reference. No mating events were detected under these environmental conditions. These findings supported assumptions that <em>K. phaffii</em> is highly specialized and not a ubiquitous microorganism as described for other yeasts. Finally, intrigued by the low survival outside optimal laboratory conditions, <em>K. phaffii</em> biomass was evaluated in mesophilic anaerobic digestion processes for energy production. Enhanced methane yields and a concomitant loss of viable <em>K. phaffii</em> cells were demonstrated.</div></div>","PeriodicalId":19190,"journal":{"name":"New biotechnology","volume":"90 ","pages":"Pages 1-12"},"PeriodicalIF":4.9,"publicationDate":"2025-08-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144775816","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Creation of colorless transparent tilapia using CRISPR/Cas9 mediated multi-gene mutation","authors":"Guangyuan Liang ,&nbsp;Baoyue Lu ,&nbsp;Shengfei Dai,&nbsp;Minghui Li,&nbsp;Jiawen Yao,&nbsp;Hao Liu,&nbsp;Xiayue Liu,&nbsp;Xingyong Liu,&nbsp;Deshou Wang","doi":"10.1016/j.nbt.2025.07.009","DOIUrl":"10.1016/j.nbt.2025.07.009","url":null,"abstract":"<div><div>Transparent mutant fish have been obtained from small-sized fish (medaka, zebrafish, and killifish), and are often used as experimental models in biological and medical research. However, transparent fish have never been created in medium- or large-sized fish through gene editing. In this study, mutants without xanthophores, erythrophores and pigmented melanophores were first obtained by crossing to form <em>tyrb;csf1ra</em> double mutant tilapia. Subsequently, single mutants lacking reflective platelets without visible iridophores were obtained by mutation of <em>pnp4a</em> and <em>tfec</em> using CRISPR/Cas9 gene editing, the recovery of iridophores were observed in <em>pnp4a</em> mutants but not in <em>tfec</em> mutants in the later stage. In addition, we also found that <em>tfec</em> mutation led to a decrease in number of melanophores and diameter of melanophores/erythrophores. Finally, transparent triple mutant homozygotes without visible pigment cells were obtained by triple mutation of <em>tyrb</em>;<em>csf1ra</em>;<em>pnp4a</em> (named <em>amber</em>) and <em>tyrb</em>;<em>csf1ra</em>;<em>tfec</em> (named <em>ruby</em>), through crossing of double and single mutants. The two mutant lines were no longer transparent at 60 dpf (days post fertilization) and 120 dpf, respectively, due to the recovery of iridophores in the former, and thickening of the body wall in the latter. In summary, this study created two transparent lines without visible pigment cells through aggregation of multiple gene mutation and crossing. The transparent fish is suitable for in vivo imaging, and is currently the only medium-sized transparent fish obtained through gene editing.</div></div>","PeriodicalId":19190,"journal":{"name":"New biotechnology","volume":"89 ","pages":"Pages 163-176"},"PeriodicalIF":4.9,"publicationDate":"2025-07-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144768811","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Biology-aware machine learning for culture medium optimization","authors":"Takamasa Hashizume ,&nbsp;Bei-Wen Ying","doi":"10.1016/j.nbt.2025.07.006","DOIUrl":"10.1016/j.nbt.2025.07.006","url":null,"abstract":"<div><div>Cell culture technologies are widely used in academia and industry, yet optimizing culture media remains an art due to the complexity of cell-medium interactions. Machine learning has emerged as a promising solution, but it is hindered by biological fluctuations and experimental errors. To address these issues, we developed a medium optimization platform that integrates simplified and effective experimental manipulation, error-aware data processing for model training, predictive model construction to enhance accuracy and avoid local optimization, and an efficient optimization framework of active learning. Using this approach, we fine-tuned a 57-component serum-free medium for CHO-K1 cells, in which a total of 364 media were experimentally tested. The reformulated medium achieved approximately 60 % higher cell concentration than commercial alternatives. The improved cell culture is definitive toward CHO-K1, underscoring the platform's precision in targeted cell culture optimization. Our approach offers a robust tool for optimizing complex systems in cell culture and broader experimental studies, as well as in biomedical engineering applications.</div></div>","PeriodicalId":19190,"journal":{"name":"New biotechnology","volume":"89 ","pages":"Pages 141-151"},"PeriodicalIF":4.9,"publicationDate":"2025-07-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144721105","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Using control bias to identify initial targets for bioproduction improvement","authors":"Michael Binns ,&nbsp;Pedro de Atauri ,&nbsp;Marta Cascante ,&nbsp;Constantinos Theodoropoulos","doi":"10.1016/j.nbt.2025.07.008","DOIUrl":"10.1016/j.nbt.2025.07.008","url":null,"abstract":"<div><div>Sensitivity analysis of bioprocess metabolic reaction networks analysis allows the prediction of system parameters such as those associated with the enzyme activity of certain reaction steps which significantly affect the overall production. However, uncertainties in kinetic rate expressions and in the resulting steady-state flux distributions limit the accuracy of these predictions. Starting from minimal information (reaction stoichiometry, and external fluxes in/out of the system and potentially identification of steps at equilibrium) a new preliminary method is proposed using sampling of elasticities and metabolic fluxes to calculate the control bias. The calculated control bias identifies steps which are likely to have positive control, negative control or negligible/uncertain control. This is intended to give initial guidance before further detailed investigation is carried out, identifying targets for any organism to enhance production of valuable chemicals. As a case study, this methodology is applied to succinic acid bioproduction using <em>Actinobacillus succinogenes</em> and analysis successfully reveals the reaction steps having the greatest positive and negative influence on biosuccinic acid production.</div></div>","PeriodicalId":19190,"journal":{"name":"New biotechnology","volume":"89 ","pages":"Pages 130-140"},"PeriodicalIF":4.9,"publicationDate":"2025-07-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144718160","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Future food court in an urban shopping center can supply feedstocks for microbial protein production","authors":"Terhi Viinikanoja ,&nbsp;Eevi Haajanen ,&nbsp;Maria Pajumo,&nbsp;Amanda Lillberg,&nbsp;Emilia Nordlund,&nbsp;Anneli Ritala","doi":"10.1016/j.nbt.2025.07.007","DOIUrl":"10.1016/j.nbt.2025.07.007","url":null,"abstract":"<div><div>Food courts in urban shopping centers can be future sources of feedstock for microbial protein production. This study explored whether filamentous fungi <em>Trichoderma reesei</em>, <em>Paecilomyces variotii</em> and <em>Rhizopus oligosporus</em> can be cultivated on growth media prepared from orange peels, soft drink waste-mix, expired bread or spent coffee grounds, all existing and potential by-products of future food courts. Furthermore, artificial urine to describe human urine as a future nitrogen source was investigated. Side stream pretreatments facilitated sugar releases up to 171 g L⁻¹ (expired bread). Cultivation media were prepared from pretreated side streams with 40 g L⁻¹ sugar. Flask cultivations supplemented with nitrogen and micronutrient increased fungal biomass production. From the nitrogen sources tested, yeast extract (10 g L⁻¹) proved effective in media based on soft drink waste-mix and orange peel juice. For the latter artificial urine (5 g L⁻¹ urea) worked as well. The effects of these nitrogen supplements, along with pH, on biomass production were further optimized using design of experiment methodology and validated in lab-scale bioreactor cultivations. The bioreactor cultivations of <em>P. variotii</em> and <em>T. reesei</em> with soft drink waste-mix or orange peel juice supplemented with yeast extract (13 – 15 g L⁻¹) resulted in good biomass production up to 37.8 g L⁻¹ and 15 g L⁻¹ dry weight with total protein contents reaching 25 % and 35 % for <em>P. variotii</em> and <em>T. reesei</em>, respectively. Amino acid analyses revealed balanced essential amino acid distributions for human consumption, confirming that future food court by-products are viable substrates for sustainable fungal protein production in a circular economy.</div></div>","PeriodicalId":19190,"journal":{"name":"New biotechnology","volume":"89 ","pages":"Pages 152-162"},"PeriodicalIF":4.9,"publicationDate":"2025-07-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144718159","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Conditional guide RNA deactivation by mRNA and small molecule triggers in Saccharomyces cerevisiae","authors":"Chenggang Xi ,&nbsp;Stephen Chiu ,&nbsp;William E. Voje ,&nbsp;James M. Carothers ,&nbsp;Tae Seok Moon","doi":"10.1016/j.nbt.2025.07.004","DOIUrl":"10.1016/j.nbt.2025.07.004","url":null,"abstract":"<div><div>CRISPR interference (CRISPRi) technologies have revolutionized bioengineering by providing precise tools for gene expression modulation, enabling targeted gene perturbation and metabolic pathway optimization. Despite these advances, achieving dynamic control over gene expression by CRISPR-based regulation remains a challenge due to its inherently static nature. Utilizing toehold-mediated strand displacement and ligand-responsive ribozymes (aptazymes), this study introduces switchable guide RNAs (gRNAs) that facilitate tunable gene expression mediated by mRNA or small molecule signals. We demonstrate complete silencing of gRNA via strategically designed 5’ or 3’ extensions that impede the gRNA spacer or the dCas9 handle, with subsequent restoration of function through sequestration or cleavage of the obstructive sequence. The resulting toehold-embedded or aptazyme-embedded gRNAs can be deactivated by specific signals, including two full-length translatable mRNAs and two small molecule triggers, thereby lifting CRISPRi repression on targeted genes. This modular approach allows for gRNA-based biocomputing through multi-layer or multi-input genetic logic gates in <em>Saccharomyces cerevisiae</em>. Offering a versatile strategy for post-CRISPR regulation in response to environmental signals or cellular states, this methodology expands the toolkit in eukaryotic systems for reversible control of gene expression.</div></div>","PeriodicalId":19190,"journal":{"name":"New biotechnology","volume":"89 ","pages":"Pages 105-118"},"PeriodicalIF":4.5,"publicationDate":"2025-07-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144668090","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"PHB in cyanobacteria: analyzing production through images processing and FT-IR techniques.","authors":"Paula Villar Sola ,&nbsp;Juan Fernández Montenegro ,&nbsp;Sandra Iglesias Moreira ,&nbsp;Francisco Rodríguez Lorenzo ,&nbsp;Philippe Vandervorst ,&nbsp;Erika Pancorbo González ,&nbsp;Miguel Placer Lorenzo ,&nbsp;Inés Pérez Couñago ,&nbsp;Santiago Muíños Landín ,&nbsp;Luz Herrero Castilla ,&nbsp;Julio Illade Quinteiro ,&nbsp;Juan A. Álvarez Rodríguez ,&nbsp;Beatriz Altamira Algarra ,&nbsp;Eva Gonzalez Flo ,&nbsp;Joan García","doi":"10.1016/j.nbt.2025.07.003","DOIUrl":"10.1016/j.nbt.2025.07.003","url":null,"abstract":"<div><div>Cyanobacteria have gained significant attention in recent years due to their ability to produce a variety of valuable compounds. One such compound is polyhydroxybutyrate (PHB), a biodegradable polymer with immense potential in various industrial applications. Given that PHB is stored intracellularly, a dedicated process is needed to extract and measure the biopolymer content. Nevertheless, this process is time consuming and requires environmental hazardous chemicals, such as chloroform. In the present work, we present two complementary methods developed to analyze and quantify PHB production in cyanobacteria microbiomes. The first one consists in an image processing applied on images obtained from Transmission Electronic Microscope (TEM), that can potentially be applied to others type of microscope images as confocal, for qualitative assessment. In this case, a segmentation process allows differentiating PHB grains inside cyanobacteria cells. A metric is then established by computing pixels area taken up by PHB in the whole image and in cyanobacteria cells. A good correlation (higher than 0.65) is observed for all indicators as regard to PHB content. The second method relies on Fourier-transform infrared (FTIR) spectroscopy, as a non-destructive and rapid method to analyze PHB. Absorption peaks due to carbonyl, and Amide I and II group characteristics of monomer structure in PHB and cyanobacteria´s protein are observed. A correlation coefficient r² of 0.96 is reached with the linear regression. A comparison between the two techniques is presented and their main advantages for PHB production optimization are explained.</div><div>-------------------------------------------------------------------------------------------------------------------------</div></div>","PeriodicalId":19190,"journal":{"name":"New biotechnology","volume":"89 ","pages":"Pages 119-129"},"PeriodicalIF":4.5,"publicationDate":"2025-07-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144668091","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Model-based engineering of Bacillus methanolicus towards de novo polyamine bioproduction from methanol","authors":"Luciana Fernandes Brito ,&nbsp;Adshaha Arampu ,&nbsp;Fernando Pérez-García ,&nbsp;Fatma Ece Altınışık Kaya ,&nbsp;Nihat Alpagu Sayar ,&nbsp;Berna Sariyar Akbulut ,&nbsp;Trygve Brautaset","doi":"10.1016/j.nbt.2025.07.001","DOIUrl":"10.1016/j.nbt.2025.07.001","url":null,"abstract":"<div><div>This study explores the one-carbon feedstock methanol to bolster sustainable bioproduction of valuable polyamines. <em>Bacillus methanolicus</em> MGA3, a methylotroph, stands out as a promising host due to its aptitude for employing methanol to synthesize various chemicals. Our approach used flux balance analysis (FBA) to leverage native <em>B. methanolicus</em> pathways for biosynthesis of the polyamines putrescine and spermidine. Despite possessing the genetic repertoire required for their production, <em>B. methanolicus</em> naturally secretes spermidine but not putrescine. Therefore, we created recombinant strains overexpressing endogenous and heterologous genes for putrescine biosynthesis via the arginine decarboxylase pathway, including arginine decarboxylase (<em>speA</em>) and agmatinase (<em>speB</em>). The <em>B. methanolicus</em> strain PUTEc, overexpressing <em>speAB</em> from <em>Escherichia coli</em> rather than native ones, achieved putrescine production of 47.5 ± 0.8 μM in shake flasks. Towards spermidine production, FBA pointed to overexpressing <em>S</em>-adenosylmethionine decarboxylase (<em>speH</em>) and spermidine synthase (<em>speE</em>) in the PUTEc strain. As a result, production of 83.9 ± 2.7 μM spermidine was achieved from methanol. Subsequently, the PUTEc strain underwent FBA-based screening involving precursor supplementation extracellularly in the growth medium or intracellularly by gene co-overexpression to enhance putrescine production. Overexpression of the endogenous ornithine biosynthesis pathway in the PUTEc strain yielded the highest methanol-based polyamine production in this study: 137.7 ± 1.8 μM putrescine in small-scale shake flask conditions. With further improvements in titer towards several grams per litre, this sustainable bioprocess could supply the steadily growing ∼ $500 M putrescine market. To our knowledge, this is the first proof-of-concept study towards production of putrescine and spermidine from methanol.</div></div>","PeriodicalId":19190,"journal":{"name":"New biotechnology","volume":"89 ","pages":"Pages 91-104"},"PeriodicalIF":4.5,"publicationDate":"2025-07-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144626786","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}