New biotechnology最新文献

{"title":"Exploring environmental factors affecting assistive technology strategies in mathematics learning for students with physical disabilities.","authors":"Paola Esquivel, Lynn McGarvey, Shanon Phelan, Kim Adams","doi":"10.1080/17483107.2022.2062465","DOIUrl":"10.1080/17483107.2022.2062465","url":null,"abstract":"<p><strong>Purpose: </strong>To explore the environmental facilitators and barriers affecting K-12 students with physical disabilities when using two assistive technology (AT) strategies, LEGO Mindstorms robots and a computer, in mathematics lessons.</p><p><strong>Materials and methods: </strong>A qualitative multiple case study with three students with physical disabilities was conducted. The participants did five lessons in concepts they were studying in school with each AT strategy in a controlled environment. Observations of the lessons, and parent and teacher interviews after the last session were collected.</p><p><strong>Results: </strong>The AT strategies acted as facilitators because they were easy to use, participants could participate actively, and parents and school staff wanted to implement them. However, the strategies presented some barriers since the students required more time to complete the lessons with the robot, and some computer programs were not compatible with the students' skills. Also, barriers such as lack of technical knowledge on the part of parents and teachers, distractions in the environment, and funding issues were reported.</p><p><strong>Conclusion: </strong>This study may be useful for rehabilitation staff and teachers who are considering implementing AT to support the participation of students with disabilities. Personnel needs to be trained to use the AT strategies, and several modifications may be necessary for the satisfactory use of the devices.IMPLICATIONS FOR REHABILITATIONHands-on manipulation promotes the active learning of early mathematical concepts. Students with disabilities may have the opportunity to participate in active learning through assistive technology (AT).Knowledge about environmental factors that may facilitate or limit the use of AT in the classroom will help teaching staff make decisions when implementing these strategies with students with disabilities.The use of an AT strategy may depend on the objectives the rehabilitation staff and teachers want to achieve during a task. For instance, the robot could be used for tailoring a task according to the student's needs and abilities. On the other hand, computer programs could be considered if time and ease of use are important factors.</p>","PeriodicalId":19190,"journal":{"name":"New biotechnology","volume":"27 1","pages":"66-77"},"PeriodicalIF":2.2,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"76854745","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Novel β-galactosidase activity and first crystal structure of Glycoside Hydrolase family 154","authors":"Lisanne Hameleers ,&nbsp;Tjaard Pijning ,&nbsp;Brandon B. Gray ,&nbsp;Régis Fauré ,&nbsp;Edita Jurak","doi":"10.1016/j.nbt.2023.12.011","DOIUrl":"10.1016/j.nbt.2023.12.011","url":null,"abstract":"<div><p>Polysaccharide Utilization Loci (PULs) are physically linked gene clusters conserved in the Gram-negative phylum of Bacteroidota and are valuable sources for Carbohydrate Active enZyme (CAZyme) discovery. This study focuses on BD-β-Gal, an enzyme encoded in a metagenomic PUL and member of the Glycoside Hydrolase family 154 (GH154). BD-β-Gal showed <em>exo-</em>β-galactosidase activity with regiopreference for hydrolyzing β-<span>d</span>-(1,6) glycosidic linkages. Notably, it exhibited a preference for <span>d</span>-glucopyranosyl (<span>d</span>-Glc<em>p</em>) over <span>d</span>-galactopyranosyl (<span>d</span>-Gal<em>p</em>) and <span>d</span>-fructofuranosyl (<span>d</span>-Fru<em>f</em>) at the reducing end of the investigated disaccharides. In addition, we determined the high resolution crystal structure of BD-β-Gal, thus providing the first structural characterization of a GH154 enzyme. Surprisingly, this revealed an (α/α)₆ topology, which has not been observed before for β-galactosidases. BD-β-Gal displayed low structural homology with characterized CAZymes, but conservation analysis suggested that the active site was located in a central cavity, with conserved E73, R252, and D253 as putative catalytic residues. Interestingly, BD-β-Gal has a tetrameric structure and a flexible loop from a neighboring protomer may contribute to its reaction specificity. Finally, we showed that the founding member of GH154, BT3677 from <em>Bacteroides thetaiotaomicron</em>, described as β-glucuronidase, displayed <em>exo</em>-β-galactosidase activity like BD-β-Gal but lacked a tetrameric structure.</p></div>","PeriodicalId":19190,"journal":{"name":"New biotechnology","volume":"80 ","pages":"Pages 1-11"},"PeriodicalIF":5.4,"publicationDate":"2023-12-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S1871678423000791/pdfft?md5=6b953918e4fd385feafde80b47c680e7&pid=1-s2.0-S1871678423000791-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139074592","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Co-culture platform for tuning of cancer receptor density allows for evaluation of bispecific immune cell engagers","authors":"Aman Mebrahtu ,&nbsp;Gustav Aniander ,&nbsp;Alessandro Mega ,&nbsp;Mona Moradi Barzadd ,&nbsp;Niklas Berndt Thalén ,&nbsp;Lindvi Gudmundsdotter ,&nbsp;Eva Backström Rydin ,&nbsp;Anna Sandegren ,&nbsp;Fredrik Y. Frejd ,&nbsp;Johan Rockberg","doi":"10.1016/j.nbt.2023.12.012","DOIUrl":"10.1016/j.nbt.2023.12.012","url":null,"abstract":"<div><p>Cancer immunotherapy, where a patient’s immune system is harnessed to eradicate cancer cells selectively, is a leading strategy for cancer treatment. However, successes with immune checkpoint inhibitors (ICI) are hampered by reported systemic and organ-specific toxicities and by two-thirds of the patients being non-responders or subsequently acquiring resistance to approved ICIs. Hence substantial efforts are invested in discovering novel targeted immunotherapies aimed at reduced side-effects and improved potency. One way is utilizing the dual targeting feature of bispecific antibodies, which have made them increasingly popular for cancer immunotherapy. Easy and predictive screening methods for activation ranking of candidate drugs in tumor contra non-tumor environments are however lacking. Herein, we present a cell-based assay mimicking the tumor microenvironment by co-culturing B cells with engineered human embryonic kidney 293 T cells (HEK293T), presenting a controllable density of platelet-derived growth factor receptor β (PDGFRβ). A target density panel with three different surface protein levels on HEK293T cells was established by genetic constructs carrying regulatory elements limiting RNA translation of PDGFRβ. We employed a bispecific antibody-affibody construct called an AffiMab capable of binding PDGFRβ on cancer cells and CD40 expressed by B cells as a model. Specific activation of CD40-mediated signaling of immune cells was demonstrated with the two highest receptor-expressing cell lines, Level 2/3 and Level 4, while low-to-none in the low-expressing cell lines. The concept of receptor tuning and the presented co-culture protocol may be of general utility for assessing and developing novel bi-specific antibodies for immuno-oncology applications.</p></div>","PeriodicalId":19190,"journal":{"name":"New biotechnology","volume":"79 ","pages":"Pages 120-126"},"PeriodicalIF":5.4,"publicationDate":"2023-12-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S1871678423000808/pdfft?md5=f8e128c85cbe654f910fcdf16afac59f&pid=1-s2.0-S1871678423000808-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139070860","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Biorefinery of brewery spent grain to obtain bioproducts with high value-added in the market","authors":"David Outeiriño ,&nbsp;Iván Costa-Trigo ,&nbsp;Aida Ochogavias ,&nbsp;Ricardo Pinheiro de Souza Oliveira ,&nbsp;Nelson Pérez Guerra ,&nbsp;José Manuel Salgado ,&nbsp;José Manuel Domínguez","doi":"10.1016/j.nbt.2023.12.010","DOIUrl":"10.1016/j.nbt.2023.12.010","url":null,"abstract":"<div><p>The brewery industry is under economic and environmental pressure to minimize residual management costs, particularly brewery spent grain (BSG), which accounts for 80–85% (w/w) of the total by-products generated. BSG is a lignocellulosic material primarily composed of carbohydrates, proteins and lipids. Developing a biorefinery model for conversion of BSG into value-added products is a plausible idea. Previous work optimized the pretreatment of BSG with the ionic liquid [N_1112OH][Gly] and further release of fermentable sugar-containing solutions by enzymatic hydrolysis, using an enzymatic cocktail obtained by solid-state fermentation of BSG with <em>Aspergillus brasiliensis</em> CECT 2700 and <em>Trichoderma reesei</em> CECT 2414. The current work ends the biorefinery process, studying the fermentation of these culture media with two LAB strains, <em>Lactobacillus pentosus</em> CECT 4023 and <em>Lactobacillus plantarum</em> CECT 221, from which the production of organic acids, bacteriocins, and microbial biosurfactants (mBS) was obtained. In addition to the bacteriocin activity observed, a mass balance of the whole biorefinery process quantified the production of 106.4 g lactic acid and 6.76 g mBS with <em>L. plantarum</em> and 116.1 g lactic acid and 4.65 g mBS with <em>L. pentosus</em> from 1 kg of dry BSG. Thus, BSG shows a great potential for waste valorization, playing a major role in the implementation of biomass biorefineries in circular bioeconomy.</p></div>","PeriodicalId":19190,"journal":{"name":"New biotechnology","volume":"79 ","pages":"Pages 111-119"},"PeriodicalIF":5.4,"publicationDate":"2023-12-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S187167842300078X/pdfft?md5=a76eaa8a6c4dd12b9b7485351fb50cbf&pid=1-s2.0-S187167842300078X-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139070750","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"In pursuit of a minimal CHO genome: Establishment of large-scale genome deletions","authors":"Tobias Jerabek ,&nbsp;Linus Weiß ,&nbsp;Hannah Fahrion ,&nbsp;Nikolas Zeh ,&nbsp;Nadja Raab ,&nbsp;Benjamin Lindner ,&nbsp;Simon Fischer ,&nbsp;Kerstin Otte","doi":"10.1016/j.nbt.2023.12.007","DOIUrl":"10.1016/j.nbt.2023.12.007","url":null,"abstract":"<div><p>Chinese hamster ovary (CHO) cells are the most commonly used mammalian cell line for the production of complex therapeutic glycoproteins. As CHO cells have evolved as part of a multicellular organism, they harbor many cellular functions irrelevant for their application as production hosts in industrial bioprocesses. Consequently, CHO cells have been the target for numerous genetic engineering efforts in the past, but a tailored host cell chassis holistically optimized for its specific task in a bioreactor is still missing. While the concept of genome reduction has already been successfully applied to bacterial production cells, attempts to create higher eukaryotic production hosts exhibiting reduced genomes have not been reported yet. Here, we present the establishment and application of a large-scale genome deletion strategy for targeted excision of large genomic regions in CHO cells. We demonstrate the feasibility of genome reduction in CHO cells using optimized CRISPR/Cas9 based experimental protocols targeting large non-essential genomic regions with high efficiency. Achieved genome deletions of non-essential genetic regions did not introduce negative effects on bioprocess relevant parameters, although we conducted the largest reported genomic excision of 864 kilobase pairs in CHO cells so far. The concept presented serves as a directive to accelerate the development of a significantly genome-reduced CHO host cell chassis paving the way for a next generation of CHO cell factories.</p></div>","PeriodicalId":19190,"journal":{"name":"New biotechnology","volume":"79 ","pages":"Pages 100-110"},"PeriodicalIF":5.4,"publicationDate":"2023-12-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S1871678423000754/pdfft?md5=61771dcaec320ae3b670e1e43b896980&pid=1-s2.0-S1871678423000754-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139058562","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Post-translational secretion stress regulation in Bacillus subtilis is controlled by intra- and extracellular proteases","authors":"Ayşegül Öktem,&nbsp;Dicky A. Pranoto,&nbsp;Jan Maarten van Dijl","doi":"10.1016/j.nbt.2023.12.009","DOIUrl":"10.1016/j.nbt.2023.12.009","url":null,"abstract":"<div><p>The Gram-positive bacterium <em>Bacillus subtilis</em> is a prolific producer of industrial enzymes that are effectively harvested from the fermentation broth. However, the high capacity of <em>B. subtilis</em> for protein secretion has so far not been exploited to the full due to particular bottlenecks, including product degradation by extracellular proteases and counterproductive secretion stress responses. To unlock the <em>Bacillus</em> secretion pathway for difficult-to-produce proteins, various cellular interventions have been explored, including genome engineering. Our previous research has shown a superior performance of genome-reduced <em>B. subtilis</em> strains in the production of staphylococcal antigens compared to the parental strain 168. This was attributed, at least in part, to redirected secretion stress responses, including the presentation of elevated levels of the quality control proteases HtrA and HtrB that also catalyse protein folding. Here we show that this relates to the elimination of two homologous serine proteases, namely the cytosolic protease AprX and the extracellular protease AprE. This unprecedented posttranslational regulation of secretion stress effectors, like HtrA and HtrB, by the concerted action of cytosolic and extracellular proteases has important implications for the biotechnological application of microbial cell factories. In <em>B. subtilis</em>, this conclusion is underscored by extracellular degradation of the staphylococcal antigen IsaA by both AprX and AprE. Extracellular activity of the cytosolic protease AprX is remarkable since it shows that not only extracellular, but also intracellular proteases impact extracellular product levels. We therefore conclude that intracellular proteases represent new targets for improved recombinant protein production in microbial cell factories like <em>B. subtilis</em>.</p></div>","PeriodicalId":19190,"journal":{"name":"New biotechnology","volume":"79 ","pages":"Pages 71-81"},"PeriodicalIF":5.4,"publicationDate":"2023-12-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S1871678423000778/pdfft?md5=4b2e3c074f4104811bb12d8e91b10a92&pid=1-s2.0-S1871678423000778-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139070798","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Inefficient transcription is a production bottleneck for artificial therapeutic BiTE® proteins","authors":"Tobias Jerabek ,&nbsp;Madina Burkhart ,&nbsp;Selina Goetz ,&nbsp;Benedikt Greck ,&nbsp;Anika Menthe ,&nbsp;Ruediger Neef ,&nbsp;Kerstin Otte","doi":"10.1016/j.nbt.2023.12.008","DOIUrl":"10.1016/j.nbt.2023.12.008","url":null,"abstract":"<div><p>Antibodies are potent biopharmaceuticals used to treat severe diseases, including cancers. During the past decade, more complex modalities have been developed including bispecific T-cell engager (BiTE®) molecules, e.g. by Amgen. However, non-natural and complex molecule formats often prove to be difficult-to-express (DTE), which is the case for BiTE® molecules. Due to the growing importance of multispecific modalities such as half-life extended (HLE) BiTE® and HLE dual-targeting bispecific T-cell engager (dBiTE) molecules, this artificial class of therapeutic proteins was investigated for molecular bottlenecks in stable production cell lines, by analyzing all relevant steps of recombinant protein production. As a result, drastically reduced intracellular BiTE® molecule-encoding mRNA levels were identified as a potential production bottleneck. Using <em>in vitro</em> transcription (IVT), the transcription rate of the BiTE® molecule-encoding mRNA was identified as the root cause for reduced amounts of intracellular mRNA. In an attempt to improve the transcription rate of a BiTE® molecule, it could be demonstrated that the artificial and special structure of the BiTE® molecule was not the rate limiting step for reduced IVT rate. However, modulation of the primary DNA sequence led to significant improvement of IVT rate. The analyses presented provide insight into the HLE BiTE® / HLE d(BiTE®) class of DTE proteins and perhaps into other classes of DTE proteins, and therefore may lead to identification of further production bottlenecks and optimization strategies to overcome manufacturability challenges associated with various complex therapeutics.</p></div>","PeriodicalId":19190,"journal":{"name":"New biotechnology","volume":"79 ","pages":"Pages 91-99"},"PeriodicalIF":5.4,"publicationDate":"2023-12-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S1871678423000766/pdfft?md5=559b80361300714655d45d6d4e3f5c43&pid=1-s2.0-S1871678423000766-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139052188","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Pre-analytical properties of different respiratory viruses for PCR-based detection: Comparative analysis of sampling devices and sample stabilization solutions","authors":"Melina Hardt ,&nbsp;Franziska Kaiser ,&nbsp;Thorsten Voss ,&nbsp;Uwe Oelmüller ,&nbsp;Kurt Zatloukal","doi":"10.1016/j.nbt.2023.12.005","DOIUrl":"10.1016/j.nbt.2023.12.005","url":null,"abstract":"<div><p>After the decline of the COVID-19 pandemic, health systems were challenged by the simultaneous prevalence of different respiratory viruses causing a wide overlap in symptoms. This increased the demand for multi-virus diagnostic tests which require suitable pre-analytical workflow solutions in order to receive valid diagnostic results. In this context, the effects of specimen storage duration and temperature on the RNA/DNA copy number stability of influenza A/B, RSV A/B, SARS-CoV-2 and adenovirus were examined for four commercially available transport swab systems and saliva collection devices. The respiratory viruses were more stable in the saliva collection devices than in the transport swab systems when stored at RT or 37 °C for up to 96 h. Moreover, no differences between viral nucleic acid stability of enveloped and non-enveloped viruses were observed. The infectivity of all enveloped viruses could be inactivated by the saliva collection device from PreAnalytiX. The Norgen saliva device completely inactivated influenza A/B, while RSV A/B were partially inactivated. The non-enveloped adenovirus was inactivated by a reduction factor of 10E+ 4 in both saliva collection devices. All respiratory viruses remained infectious in the transport swab systems. Two possible transport medium additives were tested which inactivated or strongly reduced viral replication of tested enveloped viruses but had no effect on the non-enveloped adenovirus. Finally the implementation of multi-target detection procedures involving a direct amplification approach was successfully tested by spike-in of all enveloped viruses simultaneously into transport swab systems. This fast and reproducible setup presents a valuable solution for future implementations in multi-virus testing strategies.</p></div>","PeriodicalId":19190,"journal":{"name":"New biotechnology","volume":"79 ","pages":"Pages 60-70"},"PeriodicalIF":5.4,"publicationDate":"2023-12-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S1871678423000730/pdfft?md5=caf3a680483bb2cb06adabb5d6aeb756&pid=1-s2.0-S1871678423000730-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139026804","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Site-directed neutralizing antibodies targeting structural sites on SARS-CoV-2 spike protein","authors":"Xiaofeng Li ,&nbsp;Liudmila Kulakova ,&nbsp;Kezzia Jones ,&nbsp;Eric A. Toth ,&nbsp;Marina Kirkland Mitchell ,&nbsp;Qiana Mendez ,&nbsp;Michael P. Weiner ,&nbsp;Thomas R. Fuerst","doi":"10.1016/j.nbt.2023.12.004","DOIUrl":"10.1016/j.nbt.2023.12.004","url":null,"abstract":"<div><p>‘Epivolve’ (<u>epi</u>tope e<u>vol</u>ution) is an innovative paratope-evolving technology using a haptenated peptide or protein immunogen as a means of directing the <em>in vivo</em> immune response to specifically targeted sites at a one amino acid residue resolution. Guided by protein structural analysis, Epivolve technology was tested to develop site-directed neutralizing antibodies (nAbs) in a systematic fashion against the SARS-CoV-2 Receptor Binding Domain (RBD). Thirteen solvent-exposed sites covering the ACE2 receptor-binding interface were targeted. Immunogens composed of each targeted site were used to immunize rabbits in separate cohorts. <em>In vivo</em> site-directed immune responses against all 13 targets were demonstrated by B cell secreted IgG and recombinant IgG testing. One site, SL13 (Y505) which mutates from tyrosine to histidine in the SARS-CoV-2 Omicron variant, was chosen as a proof-of-concept (PoC) model for further functional monoclonal antibody development. Epivolve technology demonstrated the capabilities of generating pan-variant antibodies and nAbs against the SARS-CoV-2 primary strain and the Omicron variant.</p></div>","PeriodicalId":19190,"journal":{"name":"New biotechnology","volume":"80 ","pages":"Pages 27-36"},"PeriodicalIF":5.4,"publicationDate":"2023-12-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S1871678423000729/pdfft?md5=af4e497332d22b8dd3fec32ec70fac4e&pid=1-s2.0-S1871678423000729-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138830677","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Phytostabilization of metal(loid)s by ten emergent macrophytes following a 90-day exposure to industrially contaminated groundwater","authors":"Blanca Velasco-Arroyo ,&nbsp;Sandra Curiel-Alegre ,&nbsp;Aqib Hassan Ali Khan ,&nbsp;Carlos Rumbo ,&nbsp;Daniel Pérez-Alonso ,&nbsp;Carlos Rad ,&nbsp;Herwig De Wilde ,&nbsp;Alfredo Pérez-de-Mora ,&nbsp;Rocío Barros","doi":"10.1016/j.nbt.2023.12.003","DOIUrl":"10.1016/j.nbt.2023.12.003","url":null,"abstract":"<div><p>Better understanding of macrophyte tolerance under long exposure times in real environmental matrices is crucial for phytoremediation and phytoattenuation strategies for aquatic systems. The metal(loid) attenuation ability of 10 emergent macrophyte species (<em>Carex riparia</em>, <em>Cyperus longus</em>, <em>Cyperus rotundus</em>, <em>Iris pseudacorus</em>, <em>Juncus effusus</em>, <em>Lythrum salicaria</em>, <em>Menta aquatica</em>, <em>Phragmites australis</em>, <em>Scirpus holoschoenus</em>, and <em>Typha angustifolia</em>) was investigated using real groundwater from an industrial site, over a 90-day exposure period. A “phytobial” treatment was included, with 3 plant growth-promoting rhizobacterial strains. Plants exposed to the polluted water generally showed similar or reduced aerial biomass compared to the controls, except for <em>C. riparia.</em> This species, along with <em>M. aquatica,</em> exhibited improved biomass after bioaugmentation. Phytoremediation mechanisms accounted for more than 60% of As, Cd, Cu, Ni, and Pb removal, whilst abiotic mechanisms contributed to ∼80% removal of Fe and Zn. Concentrations of metal(loid)s in the roots were generally between 10–100 times higher than in the aerial parts. The macrophytes in this work can be considered “underground attenuators”, more appropriate for rhizostabilization strategies, especially <em>L. salicaria, M. aquatica, S. holoschoenus,</em> and <em>T. angustifolia</em>. For <em>I. pseudacorus, C. longus</em>, and <em>C. riparia;</em> harvesting the aerial parts could be a complementary phytoextraction approach to further remove Pb and Zn. Of all the plants, <em>S. holoschoenus</em> showed the best balance between biomass production and uptake of multiple metal(loid)s. Results also suggest that multiple phytostrategies may be possible for the same plant depending on the final remedial aim. Phytobial approaches need to be further assessed for each macrophyte species.</p></div>","PeriodicalId":19190,"journal":{"name":"New biotechnology","volume":"79 ","pages":"Pages 50-59"},"PeriodicalIF":5.4,"publicationDate":"2023-12-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S1871678423000717/pdfft?md5=7f9945b9a05ea049aeb1c7c5437ba0c5&pid=1-s2.0-S1871678423000717-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138830676","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}