Nature Biomedical Engineering最新文献_第7页

Evolving adeno-associated viruses for gene transfer to the kidney via cross-species cycling of capsid libraries 进化的腺相关病毒通过衣壳库的跨物种循环将基因转移到肾脏

IF 28.1 1区医学

Nature Biomedical Engineering Pub Date : 2025-02-05 DOI: 10.1038/s41551-024-01341-0

Alan Rosales, Leo O. Blondel, Joshua Hull, Qimeng Gao, Nihal Aykun, Jennifer L. Peek, Alejandra Vargas, Sophia Fergione, Mingqing Song, Matthew H. Wilson, Andrew S. Barbas, Aravind Asokan

{"title":"Evolving adeno-associated viruses for gene transfer to the kidney via cross-species cycling of capsid libraries","authors":"Alan Rosales, Leo O. Blondel, Joshua Hull, Qimeng Gao, Nihal Aykun, Jennifer L. Peek, Alejandra Vargas, Sophia Fergione, Mingqing Song, Matthew H. Wilson, Andrew S. Barbas, Aravind Asokan","doi":"10.1038/s41551-024-01341-0","DOIUrl":"https://doi.org/10.1038/s41551-024-01341-0","url":null,"abstract":"The difficulty of delivering genes to the kidney has limited the translation of genetic medicines, particularly for the more than 10% of the global population with chronic kidney disease. Here we show that new variants of adeno-associated viruses (AAVs) displaying robust and widespread transduction in the kidneys of mice, pigs and non-human-primates can be obtained by evolving capsid libraries via cross-species cycling in different kidney models. Specifically, the new variants, AAV.k13 and AAV.k20, were enriched from the libraries following sequential intravenous cycling through mouse and pig kidneys, ex vivo cycling in human organoid cultures, and ex vivo machine perfusion in isolated kidneys from rhesus macaques. The two variants transduced murine kidneys following intravenous administration, with selective tropism for proximal tubules, and led to markedly higher transgene expression than parental AAV9 vectors in proximal tubule epithelial cells within human organoid cultures and in autotransplanted pig kidneys. Following ureteral delivery, AAV.k20 efficiently transduced kidneys in pigs and macaques. The AAV.k13 and AAV.k20 variants are promising vectors for therapeutic gene-transfer applications in kidney diseases and transplantation.","PeriodicalId":19063,"journal":{"name":"Nature Biomedical Engineering","volume":"123 1","pages":""},"PeriodicalIF":28.1,"publicationDate":"2025-02-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143124768","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Author Correction: Enhanced control of a brain–computer interface by tetraplegic participants via neural-network-mediated feature extraction 作者更正：通过神经网络介导的特征提取增强四肢瘫痪参与者脑机接口的控制

IF 28.1 1区医学

Nature Biomedical Engineering Pub Date : 2025-01-29 DOI: 10.1038/s41551-025-01355-2

Benyamin Haghi, Tyson Aflalo, Spencer Kellis, Charles Guan, Jorge A. Gamez de Leon, Albert Yan Huang, Nader Pouratian, Richard A. Andersen, Azita Emami

引用次数: 0

Urinary bioorthogonal reporters for the monitoring of the efficacy of chemotherapy for lung cancer and of associated kidney injury 用于监测肺癌化疗疗效及相关肾损伤的尿生物正交报告

IF 28.1 1区医学

Nature Biomedical Engineering Pub Date : 2025-01-29 DOI: 10.1038/s41551-024-01340-1

Penghui Cheng, Ziling Zeng, Jing Liu, Si Si Liew, Yuxuan Hu, Mengke Xu, Kanyi Pu

{"title":"Urinary bioorthogonal reporters for the monitoring of the efficacy of chemotherapy for lung cancer and of associated kidney injury","authors":"Penghui Cheng, Ziling Zeng, Jing Liu, Si Si Liew, Yuxuan Hu, Mengke Xu, Kanyi Pu","doi":"10.1038/s41551-024-01340-1","DOIUrl":"https://doi.org/10.1038/s41551-024-01340-1","url":null,"abstract":"The utility of urinary tests for the monitoring of the treatment efficacy and adverse events of anticancer therapies is constrained by the low concentration of relevant urinary biomarkers. Here we report, using mice with lung cancer and treated with chemotherapy, of a urinary fluorescence test for the concurrent monitoring of the levels of a tumour biomarker (cathepsin B) and of a biomarker of chemotherapy-induced kidney injury (N-acetyl-β-d-glucosaminidase). The test involves two intratracheally administered urinary reporters leveraging caged bioorthogonal click handles for the biomarker-dependent activation of ‘clickability’ and renal clearance, and the bioorthogonal click reaction of each renally cleared reporter with paired fluorescence indicators in the collected urine. In mouse models of chemotherapy-treated orthotopic lung cancer and of cisplatin-induced kidney injury, lower urinary fluorescence signals (which can be measured by a smartphone camera) for tumour and kidney injury levels positively correlated with animal weight gain and survival time. Biomarker-activated bioorthogonal click reactivity and renal clearance combined with bioorthogonally triggered fluorescence in vitro may enable specific, sensitive and rapid urinary assays for the monitoring of other physiopathological processes.","PeriodicalId":19063,"journal":{"name":"Nature Biomedical Engineering","volume":"40 1","pages":""},"PeriodicalIF":28.1,"publicationDate":"2025-01-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143055073","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Author Correction: Endocisternal interfaces for minimally invasive neural stimulation and recording of the brain and spinal cord 作者更正：用于大脑和脊髓微创神经刺激和记录的腔内界面

IF 28.1 1区医学

Nature Biomedical Engineering Pub Date : 2025-01-27 DOI: 10.1038/s41551-025-01356-1

Joshua C. Chen, Abdeali Dhuliyawalla, Robert Garcia, Ariadna Robledo, Joshua E. Woods, Fatima Alrashdan, Sean O’Leary, Adam Husain, Anthony Price, Scott Crosby, Michelle M. Felicella, Ajay K. Wakhloo, Patrick Karas, Nicole Provenza, Wayne Goodman, Sameer A. Sheth, Sunil A. Sheth, Jacob T. Robinson, Peter Kan

引用次数: 0

A generalist model for enhancing brain MRIs 增强脑核磁共振成像的通才模型

IF 28.1 1区医学

Nature Biomedical Engineering Pub Date : 2025-01-24 DOI: 10.1038/s41551-024-01320-5

Yael Balbastre, Bruce Fischl

引用次数: 0

Author Correction: Patterned gastrointestinal monolayers with bilateral access as observable models of parasite gut infection 作者更正：具有双侧通道的胃肠道单层模式作为寄生虫肠道感染的可观察模型

IF 28.1 1区医学

Nature Biomedical Engineering Pub Date : 2025-01-20 DOI: 10.1038/s41551-025-01351-6

Moritz Hofer, Maria A. Duque-Correa, Matthias P. Lutolf

引用次数: 0

Author Correction: Enhancing the immunogenicity of lipid-nanoparticle mRNA vaccines by adjuvanting the ionizable lipid and the mRNA 作者更正：通过佐剂可电离脂质和mRNA来增强脂质纳米颗粒mRNA疫苗的免疫原性

IF 26.8 1区医学

Nature Biomedical Engineering Pub Date : 2025-01-16 DOI: 10.1038/s41551-025-01347-2

Bowen Li, Allen Yujie Jiang, Idris Raji, Caroline Atyeo, Theresa M. Raimondo, Akiva G. R. Gordon, Luke H. Rhym, Tahoura Samad, Corina MacIsaac, Jacob Witten, Haseeb Mughal, Taras M. Chicz, Yue Xu, Ryan P. McNamara, Sangeeta Bhatia, Galit Alter, Robert Langer, Daniel G. Anderson