Molecular Microbiology最新文献_第9页

LC3B labeling of the parasitophorous vacuole membrane of Plasmodium berghei liver stage parasites depends on the V-ATPase and ATG16L1 伯格希氏疟原虫肝期寄生虫寄生液泡膜的 LC3B 标记取决于 V-ATP 酶和 ATG16L1

IF 3.6 2区生物学

Molecular Microbiology Pub Date : 2024-04-04 DOI: 10.1111/mmi.15259

Annina Bindschedler, Jacqueline Schmuckli-Maurer, Sophie Buchser, Tara D. Fischer, Rahel Wacker, Tim Davalan, Jessica Brunner, Volker T. Heussler

{"title":"LC3B labeling of the parasitophorous vacuole membrane of Plasmodium berghei liver stage parasites depends on the V-ATPase and ATG16L1","authors":"Annina Bindschedler, Jacqueline Schmuckli-Maurer, Sophie Buchser, Tara D. Fischer, Rahel Wacker, Tim Davalan, Jessica Brunner, Volker T. Heussler","doi":"10.1111/mmi.15259","DOIUrl":"https://doi.org/10.1111/mmi.15259","url":null,"abstract":"The protozoan parasite Plasmodium, the causative agent of malaria, undergoes an obligatory stage of intra-hepatic development before initiating a blood-stage infection. Productive invasion of hepatocytes involves the formation of a parasitophorous vacuole (PV) generated by the invagination of the host cell plasma membrane. Surrounded by the PV membrane (PVM), the parasite undergoes extensive replication. During intracellular development in the hepatocyte, the parasites provoke the Plasmodium-associated autophagy-related (PAAR) response. This is characterized by a long-lasting association of the autophagy marker protein, and ATG8 family member, LC3B with the PVM. LC3B localization at the PVM does not follow the canonical autophagy pathway since upstream events specific to canonical autophagy are dispensable. Here, we describe that LC3B localization at the PVM of Plasmodium parasites requires the V-ATPase and its interaction with ATG16L1. The WD40 domain of ATG16L1 is crucial for its recruitment to the PVM. Thus, we provide new mechanistic insight into the previously described PAAR response targeting Plasmodium liver stage parasites.","PeriodicalId":19006,"journal":{"name":"Molecular Microbiology","volume":null,"pages":null},"PeriodicalIF":3.6,"publicationDate":"2024-04-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140346497","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The Lrs14 family of DNA-binding proteins as nucleoid-associated proteins in the Crenarchaeal order Sulfolobales Lrs14 DNA 结合蛋白家族是 Crenarchaeal Sulfolobales 目中的核相关蛋白

IF 3.6 2区生物学

Molecular Microbiology Pub Date : 2024-04-03 DOI: 10.1111/mmi.15260

Veerke De Kock, Eveline Peeters, Rani Baes

{"title":"The Lrs14 family of DNA-binding proteins as nucleoid-associated proteins in the Crenarchaeal order Sulfolobales","authors":"Veerke De Kock, Eveline Peeters, Rani Baes","doi":"10.1111/mmi.15260","DOIUrl":"https://doi.org/10.1111/mmi.15260","url":null,"abstract":"Organization of archaeal chromatin combines bacterial, eukaryotic, and unique characteristics. Many archaeal lineages harbor a wide diversity of small and highly expressed nucleoid-associated proteins, which are involved in DNA structuring. In Sulfolobales, representing model organisms within the Crenarchaeota, Sul7d, Cren7, Sul10a, and Sul12a are well-characterized nucleoid-associated proteins. Here, we combine evidence that the Lrs14 family of DNA binders is part of the repertoire of nucleoid-associated proteins in Sulfolobales. Lrs14-encoding genes are widespread within genomes of different members of the Sulfolobales, typically encoded as four to nine homologs per genome. The Lrs14 proteins harbor a winged helix-turn-helix DNA-binding domain and are typified by a coiled–coil dimerization. They are characterized by distinct sequence- and structure-based features, including redox-sensitive motifs and residues targeted for posttranslational modification, allowing a further classification of the family into five conserved clusters. Lrs14-like proteins have unique DNA-organizing properties. By binding to the DNA nonsequence specifically and in a highly cooperative manner, with a slight preference for AT-rich promoter regions, they introduce DNA kinks and are able to affect transcription of adjacent transcription units either positively or negatively. Genes encoding Lrs14-type proteins display considerable differential expression themselves in response to various stress conditions, with certain homologs being specific to a particular stressor. Taken together, we postulate that members of the Lrs14 family can be considered nucleoid-associated proteins in Sulfolobales, combining a DNA-structuring role with a global gene expression role in response to stress conditions.","PeriodicalId":19006,"journal":{"name":"Molecular Microbiology","volume":null,"pages":null},"PeriodicalIF":3.6,"publicationDate":"2024-04-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140343323","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Whole-genome CRISPR screens to understand Apicomplexan-host interactions. 通过全基因组 CRISPR 筛选了解表皮复合菌与宿主的相互作用。

IF 3.6 2区生物学

Molecular Microbiology Pub Date : 2024-04-01 Epub Date: 2024-01-15 DOI: 10.1111/mmi.15221

Eva Hesping, Justin A Boddey

引用次数: 0

Molecular mapping of virus-infected cells with immunogold and metal-tagging transmission electron microscopy. 用免疫金和金属标记透射电子显微镜对病毒感染细胞进行分子定位。

IF 3.6 2区生物学

Molecular Microbiology Pub Date : 2024-04-01 Epub Date: 2023-10-21 DOI: 10.1111/mmi.15182

Martin Sachse, Isabel Fernández de Castro, Raquel Tenorio, Cristina Risco

{"title":"Molecular mapping of virus-infected cells with immunogold and metal-tagging transmission electron microscopy.","authors":"Martin Sachse, Isabel Fernández de Castro, Raquel Tenorio, Cristina Risco","doi":"10.1111/mmi.15182","DOIUrl":"10.1111/mmi.15182","url":null,"abstract":"Transmission electron microscopy (TEM) has been essential to study virus-cell interactions. The architecture of viral replication factories, the principles of virus assembly and the components of virus egress pathways are known thanks to the contribution of TEM methods. Specially, when studying viruses in cells, methodologies for labeling proteins and other macromolecules are important tools to correlate morphology with function. In this review, we present the most widely used labeling method for TEM, immunogold, together with a lesser known technique, metal-tagging transmission electron microscopy (METTEM) and how they can contribute to study viral infections. Immunogold uses the power of antibodies and electron dense, colloidal gold particles while METTEM uses metallothionein (MT), a metal-binding protein as a clonable tag. MT molecules build gold nano-clusters inside cells when these are incubated with gold salts. We describe the necessary controls to confirm that signals are specific, the advantages and limitations of both methods, and show some examples of immunogold and METTEM of cells infected with viruses.","PeriodicalId":19006,"journal":{"name":"Molecular Microbiology","volume":null,"pages":null},"PeriodicalIF":3.6,"publicationDate":"2024-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49679941","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

An introduction to scanning transmission electron microscopy for the study of protozoans. 研究原生动物的扫描透射电子显微镜入门。

IF 3.6 2区生物学

Molecular Microbiology Pub Date : 2024-04-01 Epub Date: 2023-12-23 DOI: 10.1111/mmi.15213

Sylvain Trépout, Marie-Laure Sgarra, Sergio Marco, Georg Ramm

{"title":"An introduction to scanning transmission electron microscopy for the study of protozoans.","authors":"Sylvain Trépout, Marie-Laure Sgarra, Sergio Marco, Georg Ramm","doi":"10.1111/mmi.15213","DOIUrl":"10.1111/mmi.15213","url":null,"abstract":"Since its inception in the 1930s, transmission electron microscopy (TEM) has been a powerful method to explore the cellular structure of parasites. TEM usually requires samples of <100 nm thick and with protozoans being larger than 1 μm, their study requires resin embedding and ultrathin sectioning. During the past decade, several new methods have been developed to improve, facilitate, and speed up the structural characterisation of biological samples, offering new imaging modalities for the study of protozoans. In particular, scanning transmission electron microscopy (STEM) can be used to observe sample sections as thick as 1 μm thus becoming an alternative to conventional TEM. STEM can also be performed under cryogenic conditions in combination with cryo-electron tomography providing access to the study of thicker samples in their native hydrated states in 3D. This method, called cryo-scanning transmission electron tomography (cryo-STET), was first developed in 2014. This review presents the basic concepts and benefits of STEM methods and provides examples to illustrate the potential for new insights into the structure and ultrastructure of protozoans.","PeriodicalId":19006,"journal":{"name":"Molecular Microbiology","volume":null,"pages":null},"PeriodicalIF":3.6,"publicationDate":"2024-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138885449","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Genome-wide profiling of piggyBac transposon insertion mutants reveals loss of the F₁F₀ ATPase complex causes fluconazole resistance in Candida glabrata. 对piggyBac转座子插入突变体的全基因组分析表明，F1 F0 ATPase复合物的缺失会导致草绿色念珠菌对氟康唑产生抗性。

IF 3.6 2区生物学

Molecular Microbiology Pub Date : 2024-04-01 Epub Date: 2024-01-19 DOI: 10.1111/mmi.15229

Eve W L Chow, Yabing Song, Haitao Wang, Xiaoli Xu, Jiaxin Gao, Yue Wang

{"title":"Genome-wide profiling of piggyBac transposon insertion mutants reveals loss of the F1F0 ATPase complex causes fluconazole resistance in Candida glabrata.","authors":"Eve W L Chow, Yabing Song, Haitao Wang, Xiaoli Xu, Jiaxin Gao, Yue Wang","doi":"10.1111/mmi.15229","DOIUrl":"10.1111/mmi.15229","url":null,"abstract":"Invasive candidiasis caused by non-albicans species has been on the rise, with Candida glabrata emerging as the second most common etiological agent. Candida glabrata possesses an intrinsically lower susceptibility to azoles and an alarming propensity to rapidly develop high-level azole resistance during treatment. In this study, we have developed an efficient piggyBac (PB) transposon-mediated mutagenesis system in C. glabrata to conduct genome-wide genetic screens and applied it to profile genes that contribute to azole resistance. When challenged with the antifungal drug fluconazole, PB insertion into 270 genes led to significant resistance. A large subset of these genes has a role in the mitochondria, including almost all genes encoding the subunits of the F1F0 ATPase complex. We show that deleting ATP3 or ATP22 results in increased azole resistance but does not affect susceptibility to polyenes and echinocandins. The increased azole resistance is due to increased expression of PDR1 that encodes a transcription factor known to promote drug efflux pump expression. Deleting PDR1 in the atp3Δ or atp22Δ mutant resulted in hypersensitivity to fluconazole. Our results shed light on the mechanisms contributing to azole resistance in C. glabrata. This PB transposon-mediated mutagenesis system can significantly facilitate future genome-wide genetic screens.","PeriodicalId":19006,"journal":{"name":"Molecular Microbiology","volume":null,"pages":null},"PeriodicalIF":3.6,"publicationDate":"2024-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139502682","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

TbsP and TrmB jointly regulate gapII to influence cell development phenotypes in the archaeon Haloferax volcanii. TbsP和TrmB共同调控gapII，影响火山古菌（Haloferax volcanii）的细胞发育表型。

IF 3.6 2区生物学

Molecular Microbiology Pub Date : 2024-04-01 Epub Date: 2024-01-11 DOI: 10.1111/mmi.15225

Rylee K Hackley, Sungmin Hwang, Jake T Herb, Preeti Bhanap, Katie Lam, Angie Vreugdenhil, Cynthia L Darnell, Mar Martinez Pastor, Johnathan H Martin, Julie A Maupin-Furlow, Amy K Schmid

{"title":"TbsP and TrmB jointly regulate gapII to influence cell development phenotypes in the archaeon Haloferax volcanii.","authors":"Rylee K Hackley, Sungmin Hwang, Jake T Herb, Preeti Bhanap, Katie Lam, Angie Vreugdenhil, Cynthia L Darnell, Mar Martinez Pastor, Johnathan H Martin, Julie A Maupin-Furlow, Amy K Schmid","doi":"10.1111/mmi.15225","DOIUrl":"10.1111/mmi.15225","url":null,"abstract":"Microbial cells must continually adapt their physiology in the face of changing environmental conditions. Archaea living in extreme conditions, such as saturated salinity, represent important examples of such resilience. The model salt-loving organism Haloferax volcanii exhibits remarkable plasticity in its morphology, biofilm formation, and motility in response to variations in nutrients and cell density. However, the mechanisms regulating these lifestyle transitions remain unclear. In prior research, we showed that the transcriptional regulator, TrmB, maintains the rod shape in the related species Halobacterium salinarum by activating the expression of enzyme-coding genes in the gluconeogenesis metabolic pathway. In Hbt. salinarum, TrmB-dependent production of glucose moieties is required for cell surface glycoprotein biogenesis. Here, we use a combination of genetics and quantitative phenotyping assays to demonstrate that TrmB is essential for growth under gluconeogenic conditions in Hfx. volcanii. The ∆trmB strain rapidly accumulated suppressor mutations in a gene encoding a novel transcriptional regulator, which we name trmB suppressor, or TbsP (a.k.a. \"tablespoon\"). TbsP is required for adhesion to abiotic surfaces (i.e., biofilm formation) and maintains wild-type cell morphology and motility. We use functional genomics and promoter fusion assays to characterize the regulons controlled by each of TrmB and TbsP, including joint regulation of the glucose-dependent transcription of gapII, which encodes an important gluconeogenic enzyme. We conclude that TrmB and TbsP coregulate gluconeogenesis, with downstream impacts on lifestyle transitions in response to nutrients in Hfx. volcanii.","PeriodicalId":19006,"journal":{"name":"Molecular Microbiology","volume":null,"pages":null},"PeriodicalIF":3.6,"publicationDate":"2024-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11023807/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139417604","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Electron microscopy: The key to resolve RNA viruses replication organelles. 电子显微镜：解析RNA病毒复制细胞器的关键。

IF 3.6 2区生物学

Molecular Microbiology Pub Date : 2024-04-01 Epub Date: 2023-09-30 DOI: 10.1111/mmi.15173

Debora Stelitano, Mirko Cortese

引用次数: 0

Caught in the act: In situ visualization of bacterial secretion systems by cryo-electron tomography. 在行动中捕获:通过冷冻电子断层扫描细菌分泌系统的原位可视化。

IF 3.6 2区生物学

Molecular Microbiology Pub Date : 2024-04-01 Epub Date: 2023-11-17 DOI: 10.1111/mmi.15186

Camille Keck, Jost Enninga, Léa Swistak

引用次数: 0

Super-resolution fluorescence microscopy for investigating bacterial cell biology. 用于研究细菌细胞生物学的超分辨率荧光显微镜。

IF 3.6 2区生物学

Molecular Microbiology Pub Date : 2024-04-01 Epub Date: 2023-12-01 DOI: 10.1111/mmi.15203

Alexander Carsten, Manuel Wolters, Martin Aepfelbacher

引用次数: 0