Molecular Microbiology最新文献_第10页

Diverse mechanisms control amino acid-dependent environmental alkalization by Candida albicans. 控制白念珠菌依赖氨基酸进行环境碱化的机制多种多样。

IF 3.6 2区生物学

Molecular Microbiology Pub Date : 2024-04-01 Epub Date: 2024-01-04 DOI: 10.1111/mmi.15216

Fitz Gerald S Silao, Valerie Diane Valeriano, Erika Uddström, Emilie Falconer, Per O Ljungdahl

{"title":"Diverse mechanisms control amino acid-dependent environmental alkalization by Candida albicans.","authors":"Fitz Gerald S Silao, Valerie Diane Valeriano, Erika Uddström, Emilie Falconer, Per O Ljungdahl","doi":"10.1111/mmi.15216","DOIUrl":"10.1111/mmi.15216","url":null,"abstract":"Candida albicans has the capacity to neutralize acidic growth environments by releasing ammonia derived from the catabolism of amino acids. The molecular components underlying alkalization and its physiological significance remain poorly understood. Here, we present an integrative model with the cytosolic NAD+-dependent glutamate dehydrogenase (Gdh2) as the principal ammonia-generating component. We show that alkalization is dependent on the SPS-sensor-regulated transcription factor STP2 and the proline-responsive activator Put3. These factors function in parallel to derepress GDH2 and the two proline catabolic enzymes PUT1 and PUT2. Consistently, a double mutant lacking STP2 and PUT3 exhibits a severe alkalization defect that nearly phenocopies that of a gdh2-/- strain. Alkalization is dependent on mitochondrial activity and in wild-type cells occurs as long as the conditions permit respiratory growth. Strikingly, Gdh2 levels decrease and cells transiently extrude glutamate as the environment becomes more alkaline. Together, these processes constitute a rudimentary regulatory system that counters and limits the negative effects associated with ammonia generation. These findings align with Gdh2 being dispensable for virulence, and based on a whole human blood virulence assay, the same is true for C. glabrata and C. auris. Using a transwell co-culture system, we observed that the growth and proliferation of Lactobacillus crispatus, a common component of the acidic vaginal microenvironment and a potent antagonist of C. albicans, is unaffected by fungal-induced alkalization. Consequently, although Candida spp. can alkalinize their growth environments, other fungal-associated processes are more critical in promoting dysbiosis and virulent fungal growth.","PeriodicalId":19006,"journal":{"name":"Molecular Microbiology","volume":null,"pages":null},"PeriodicalIF":3.6,"publicationDate":"2024-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139098318","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Endoplasmic reticulum localized TMEM33 domain-containing protein is crucial for all life cycle stages of the malaria parasite. 内质网定位的含 TMEM33 结构域的蛋白质对疟原虫的所有生命周期阶段都至关重要。

IF 3.6 2区生物学

Molecular Microbiology Pub Date : 2024-04-01 Epub Date: 2024-01-18 DOI: 10.1111/mmi.15228

Mohd Kamil, Umit Yasar Kina, Habibe Nur Atmaca, Sinem Unal, Gozde Deveci, Pinar Burak, Ahmed S I Aly

{"title":"Endoplasmic reticulum localized TMEM33 domain-containing protein is crucial for all life cycle stages of the malaria parasite.","authors":"Mohd Kamil, Umit Yasar Kina, Habibe Nur Atmaca, Sinem Unal, Gozde Deveci, Pinar Burak, Ahmed S I Aly","doi":"10.1111/mmi.15228","DOIUrl":"10.1111/mmi.15228","url":null,"abstract":"Endoplasmic reticulum (ER) plays a pivotal role in the regulation of stress responses in multiple eukaryotic cells. However, little is known about the effector mechanisms that regulate stress responses in ER of the malaria parasite. Herein, we aimed to identify the importance of a transmembrane protein 33 (TMEM33)-domain-containing protein in life cycle of the rodent malaria parasite Plasmodium berghei. TMEM33 is an ER membrane-resident protein that is involved in regulating stress responses in various eukaryotic cells. A C-terminal tagged TMEM33 was localized in the ER throughout the blood and mosquito stages of development. Targeted deletion of TMEM33 confirmed its importance for asexual blood stages and ookinete development, in addition to its essential role for sporozoite infectivity in the mammalian host. Pilot scale analysis shows that the loss of TMEM33 results in the initiation of ER stress response and induction of autophagy. Our findings conclude an important role of TMEM33 in the development of all life cycle stages of the malaria parasite, which indicates its potential as an antimalarial target.","PeriodicalId":19006,"journal":{"name":"Molecular Microbiology","volume":null,"pages":null},"PeriodicalIF":3.6,"publicationDate":"2024-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139491860","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Mycoplasma invasion into host cells: An integrated model of infection strategy. 支原体侵入宿主细胞：感染策略的综合模型

IF 3.6 2区生物学

Molecular Microbiology Pub Date : 2024-04-01 Epub Date: 2024-01-31 DOI: 10.1111/mmi.15232

Feichen Xiu, Xinru Li, Lu Liu, Yixuan Xi, Xinchao Yi, Yumeng Li, Xiaoxing You

{"title":"Mycoplasma invasion into host cells: An integrated model of infection strategy.","authors":"Feichen Xiu, Xinru Li, Lu Liu, Yixuan Xi, Xinchao Yi, Yumeng Li, Xiaoxing You","doi":"10.1111/mmi.15232","DOIUrl":"10.1111/mmi.15232","url":null,"abstract":"Mycoplasma belong to the genus Mollicutes and are notable for their small genome sizes (500-1300 kb) and limited biosynthetic capabilities. They exhibit pathogenicity by invading various cell types to survive as intracellular pathogens. Adhesion is a crucial prerequisite for successful invasion and is orchestrated by the interplay between mycoplasma surface adhesins and specific receptors on the host cell membrane. Invasion relies heavily on clathrin- and caveolae-mediated internalization, accompanied by multiple activated kinases, cytoskeletal rearrangement, and a myriad of morphological alterations, such as membrane invagination, nuclear hypertrophy and aggregation, cytoplasmic edema, and vacuolization. Once mycoplasma successfully invade host cells, they establish resilient sanctuaries in vesicles, cytoplasm, perinuclear regions, and the nucleus, wherein specific environmental conditions favor long-term survival. Although lysosomal degradation and autophagy can eliminate most invading mycoplasmas, some viable bacteria can be released into the extracellular environment via exocytosis, a crucial factor in the prolonging infection persistence. This review explores the intricate mechanisms by which mycoplasma invades host cells and perpetuates their elusive survival, with the aim of highlighting the challenge of eradicating this enigmatic bacterium.","PeriodicalId":19006,"journal":{"name":"Molecular Microbiology","volume":null,"pages":null},"PeriodicalIF":3.6,"publicationDate":"2024-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139642560","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Expansion microscopy of apicomplexan parasites. 螺旋体寄生虫的膨胀显微镜。

IF 3.6 2区生物学

Molecular Microbiology Pub Date : 2024-04-01 Epub Date: 2023-08-11 DOI: 10.1111/mmi.15135

Benjamin Liffner, Sabrina Absalon

引用次数: 0

Illuminating bunyavirus entry into host cells with fluorescence. 用荧光照亮布尼亚病毒进入宿主细胞的过程。

IF 3.6 2区生物学

Molecular Microbiology Pub Date : 2024-04-01 Epub Date: 2023-09-13 DOI: 10.1111/mmi.15165

Yu Gu, Pierre-Yves Lozach

引用次数: 0

Functional promiscuity of small multidrug resistance transporters from Staphylococcus aureus, Pseudomonas aeruginosa, and Francisella tularensis. 来自金黄色葡萄球菌、铜绿假单胞菌和土拉弗氏菌的小型耐多药转运体的功能混杂性。

IF 3.6 2区生物学

Molecular Microbiology Pub Date : 2024-04-01 Epub Date: 2024-01-29 DOI: 10.1111/mmi.15231

Peyton J Spreacker, Andrea K Wegrzynowicz, Colin J Porter, Will F Beeninga, Sydnye Demas, Emma N Powers, Katherine A Henzler-Wildman

{"title":"Functional promiscuity of small multidrug resistance transporters from Staphylococcus aureus, Pseudomonas aeruginosa, and Francisella tularensis.","authors":"Peyton J Spreacker, Andrea K Wegrzynowicz, Colin J Porter, Will F Beeninga, Sydnye Demas, Emma N Powers, Katherine A Henzler-Wildman","doi":"10.1111/mmi.15231","DOIUrl":"10.1111/mmi.15231","url":null,"abstract":"Small multidrug resistance transporters efflux toxic compounds from bacteria and are a minimal system to understand multidrug transport. Most previous studies have focused on EmrE, the model SMR from Escherichia coli, finding that EmrE has a broader substrate profile than previously thought and that EmrE may perform multiple types of transport, resulting in substrate-dependent resistance or susceptibility. Here, we performed a broad screen to identify potential substrates of three other SMRs: PAsmr from Pseudomonas aeruginosa; FTsmr from Francisella tularensis; and SAsmr from Staphylococcus aureus. This screen tested metabolic differences in E. coli expressing each transporter versus an inactive mutant, for a clean comparison of sequence and substrate-specific differences in transporter function, and identified many substrates for each transporter. In general, resistance compounds were charged, and susceptibility substrates were uncharged, but hydrophobicity was not correlated with phenotype. Two resistance hits and two susceptibility hits were validated via growth assays and IC50 calculations. Susceptibility is proposed to occur via substrate-gated proton leak, and the addition of bicarbonate antagonizes the susceptibility phenotype, consistent with this hypothesis.","PeriodicalId":19006,"journal":{"name":"Molecular Microbiology","volume":null,"pages":null},"PeriodicalIF":3.6,"publicationDate":"2024-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11023800/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139570168","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Rok from B. subtilis: Bridging genome structure and transcription regulation. 来自枯草杆菌的 Rok：连接基因组结构和转录调控。

IF 3.6 2区生物学

Molecular Microbiology Pub Date : 2024-03-21 DOI: 10.1111/mmi.15250

Amanda M Erkelens, Bert van Erp, Wilfried J J Meijer, Remus T Dame

{"title":"Rok from B. subtilis: Bridging genome structure and transcription regulation.","authors":"Amanda M Erkelens, Bert van Erp, Wilfried J J Meijer, Remus T Dame","doi":"10.1111/mmi.15250","DOIUrl":"https://doi.org/10.1111/mmi.15250","url":null,"abstract":"Bacterial genomes are folded and organized into compact yet dynamic structures, called nucleoids. Nucleoid orchestration involves many factors at multiple length scales, such as nucleoid-associated proteins and liquid-liquid phase separation, and has to be compatible with replication and transcription. Possibly, genome organization plays an intrinsic role in transcription regulation, in addition to classical transcription factors. In this review, we provide arguments supporting this view using the Gram-positive bacterium Bacillus subtilis as a model. Proteins BsSMC, HBsu and Rok all impact the structure of the B. subtilis chromosome. Particularly for Rok, there is compelling evidence that it combines its structural function with a role as global gene regulator. Many studies describe either function of Rok, but rarely both are addressed at the same time. Here, we review both sides of the coin and integrate them into one model. Rok forms unusually stable DNA-DNA bridges and this ability likely underlies its repressive effect on transcription by either preventing RNA polymerase from binding to DNA or trapping it inside DNA loops. Partner proteins are needed to change or relieve Rok-mediated gene repression. Lastly, we investigate which features characterize H-NS-like proteins, a family that, at present, lacks a clear definition.","PeriodicalId":19006,"journal":{"name":"Molecular Microbiology","volume":null,"pages":null},"PeriodicalIF":3.6,"publicationDate":"2024-03-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140175601","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Gene transfer agents: The ambiguous role of selfless viruses in genetic exchange and bacterial evolution. 基因转移剂：无私病毒在基因交换和细菌进化中的模糊角色。

IF 3.6 2区生物学

Molecular Microbiology Pub Date : 2024-03-21 DOI: 10.1111/mmi.15251

Paul Christopher Michael Fogg

{"title":"Gene transfer agents: The ambiguous role of selfless viruses in genetic exchange and bacterial evolution.","authors":"Paul Christopher Michael Fogg","doi":"10.1111/mmi.15251","DOIUrl":"https://doi.org/10.1111/mmi.15251","url":null,"abstract":"Gene transfer agents (GTAs) are genetic elements derived from ancestral bacteriophages that have become domesticated by the host. GTAs are present in diverse prokaryotic organisms, where they can facilitate horizontal gene transfer under certain conditions. Unlike typical bacteriophages, GTAs do not exhibit any preference for the replication or transfer of the genes encoding them; instead, they exhibit a remarkable capacity to package chromosomal, and sometimes extrachromosomal, DNA into virus-like capsids and disseminate it to neighboring cells. Because GTAs resemble defective prophages, identification of novel GTAs is not trivial. The detection of candidates relies on the genetic similarity to known GTAs, which has been fruitful in α-proteobacterial lineages but challenging in more distant bacteria. Here we consider several fundamental questions: What is the true prevalence of GTAs in prokaryote genomes? Given there are high costs for GTA production, what advantage do GTAs provide to the bacterial host to justify their maintenance? How is the bacterial chromosome recognized and processed for inclusion in GTA particles? This article highlights the challenges in comprehensively understanding GTAs' prevalence, function and DNA packaging method. Going forward, broad study of atypical GTAs and use of ecologically relevant conditions are required to uncover their true impact on bacterial chromosome evolution.","PeriodicalId":19006,"journal":{"name":"Molecular Microbiology","volume":null,"pages":null},"PeriodicalIF":3.6,"publicationDate":"2024-03-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140175600","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Bright individuals: Applications of fluorescent protein-based reporter systems in single-cell cellular microbiology. 明亮的个体：基于荧光蛋白的报告系统在单细胞微生物学中的应用。

IF 3.6 2区生物学

Molecular Microbiology Pub Date : 2024-03-01 Epub Date: 2024-01-18 DOI: 10.1111/mmi.15227

Marc Schulte, Luisa Grotheer, Michael Hensel

{"title":"Bright individuals: Applications of fluorescent protein-based reporter systems in single-cell cellular microbiology.","authors":"Marc Schulte, Luisa Grotheer, Michael Hensel","doi":"10.1111/mmi.15227","DOIUrl":"10.1111/mmi.15227","url":null,"abstract":"Activation and function of virulence functions of bacterial pathogens are highly dynamic in time and space, and can show considerable heterogeneity between individual cells in pathogen populations. To investigate the complex events in host-pathogen interactions, single cell analyses are required. Fluorescent proteins (FPs) are excellent tools to follow the fate of individual bacterial cells during infection, and can also be deployed to use the pathogen as a sensor for its specific environment in host cells or host organisms. This Resources describes design and applications of dual fluorescence reporters (DFR) in cellular microbiology. DFR feature constitutively expressed FPs for detection of bacterial cells, and FPs expressed by an environmentally regulated promoter for interrogation of niche-specific cues or nutritional parameters. Variations of the basic design allow the generation of DFR that can be used to analyze, on single cell level, bacterial proliferation during infection, subcellular localization of intracellular bacteria, stress response, or persister state. We describe basic considerations for DFR design and review recent applications of DFR in cellular microbiology.","PeriodicalId":19006,"journal":{"name":"Molecular Microbiology","volume":null,"pages":null},"PeriodicalIF":3.6,"publicationDate":"2024-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139485809","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Recruitment of both the ESCRT and autophagic machineries to ejecting Mycobacterium marinum. 弹出分枝杆菌的ESCRT和自噬机制都被招募。

IF 3.6 2区生物学

Molecular Microbiology Pub Date : 2024-03-01 Epub Date: 2023-05-25 DOI: 10.1111/mmi.15075

Lilli Gerstenmaier, Ombretta Colasanti, Hannah Behrens, Margot Kolonko, Christian Hammann, Monica Hagedorn

引用次数: 0