从构象角度看细菌基因组的组织原理

IF 2.6 2区生物学 Q3 BIOCHEMISTRY & MOLECULAR BIOLOGY

Molecular Microbiology Pub Date : 2024-06-22 DOI:10.1111/mmi.15290

Sokrich Ponndara, Mounia Kortebi, Frédéric Boccard, Stéphanie Bury-Moné, Virginia S. Lioy

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引用次数: 0

摘要

细菌的染色体是大分子，需要高度压实才能装入细胞内。染色体压实必须促进和维持关键的生物过程，如基因表达和 DNA 交易（复制、重组、修复和分离）。几十年来，细菌中的染色体和染色质三维组织一直是一个难题。染色体构象捕获与深度测序（Hi-C）以及其他 "omics "方法的结合，使我们能够剖析形成细菌染色体组织的结构层，从DNA拓扑学到全局染色体结构。在此，我们回顾了使用 Hi-C 的最新发现，并讨论了细菌基因组折叠的主要特征。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

Principles of bacterial genome organization, a conformational point of view

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Principles of bacterial genome organization, a conformational point of view

Bacterial chromosomes are large molecules that need to be highly compacted to fit inside the cells. Chromosome compaction must facilitate and maintain key biological processes such as gene expression and DNA transactions (replication, recombination, repair, and segregation). Chromosome and chromatin 3D-organization in bacteria has been a puzzle for decades. Chromosome conformation capture coupled to deep sequencing (Hi-C) in combination with other “omics” approaches has allowed dissection of the structural layers that shape bacterial chromosome organization, from DNA topology to global chromosome architecture. Here we review the latest findings using Hi-C and discuss the main features of bacterial genome folding.

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来源期刊

Molecular Microbiology 生物-生化与分子生物学

CiteScore

7.20

自引率

5.60%

发文量

132

审稿时长

1.7 months

期刊介绍： Molecular Microbiology, the leading primary journal in the microbial sciences, publishes molecular studies of Bacteria, Archaea, eukaryotic microorganisms, and their viruses. Research papers should lead to a deeper understanding of the molecular principles underlying basic physiological processes or mechanisms. Appropriate topics include gene expression and regulation, pathogenicity and virulence, physiology and metabolism, synthesis of macromolecules (proteins, nucleic acids, lipids, polysaccharides, etc), cell biology and subcellular organization, membrane biogenesis and function, traffic and transport, cell-cell communication and signalling pathways, evolution and gene transfer. Articles focused on host responses (cellular or immunological) to pathogens or on microbial ecology should be directed to our sister journals Cellular Microbiology and Environmental Microbiology, respectively.