Molecular Microbiology最新文献

Correction to "Galactomannan Utilization by Cellvibrio japonicus Relies on a Single Essential α-Galactosidase Encoded by the aga27A Gene". 修正“日本Cellvibrio japonicus利用半乳糖甘露聚糖依赖于aga27A基因编码的单一α-半乳糖苷酶”。

IF 2.6 2区生物学

Molecular Microbiology Pub Date : 2025-05-29 DOI: 10.1111/mmi.15372

引用次数: 0

FlhG Cooperates With the Cell Cycle Regulator GpsB to Confine Peritrichous Flagella in B. subtilis. FlhG与细胞周期调节因子GpsB协同抑制枯草芽孢杆菌中有营养的鞭毛。

IF 3.6 2区生物学

Molecular Microbiology Pub Date : 2025-05-19 DOI: 10.1111/mmi.15375

Anita Dornes,Patrica Bedrunka,Benjamin Pillet,Dieter Kressler,Thomas Heimerl,Jan Pané-Farré,Gert Bange

{"title":"FlhG Cooperates With the Cell Cycle Regulator GpsB to Confine Peritrichous Flagella in B. subtilis.","authors":"Anita Dornes,Patrica Bedrunka,Benjamin Pillet,Dieter Kressler,Thomas Heimerl,Jan Pané-Farré,Gert Bange","doi":"10.1111/mmi.15375","DOIUrl":"https://doi.org/10.1111/mmi.15375","url":null,"abstract":"Number and arrangement of flagella, the bacterial locomotion organelles, are species-specific and serve as key taxonomic markers. The FlhG ATPase (also: YlxH, FleN), along with FlhF, plays pivotal roles in determining flagellation patterns. In Bacillus subtilis, FlhG and FlhF govern the spatial arrangement of peritrichous flagella. FlhG aids in flagellar assembly by interacting with the flagellar C-ring protein FliY, yet the molecular implications of this interaction have been unclear. Our study reveals that the ATP-dependent FlhG homodimer interacts with the C-terminal domain of GpsB, a cell cycle regulator, which recruits the peptidoglycan synthase PBP1 (also: ponA) to sites of cell wall elongation. A deletion of gpsB leads to dysregulation of the flagellation pattern mimicking the effects of a flhG deletion strain. The finding that GpsB can interact simultaneously with FlhG and PBP1, combined with the observation that GpsB and FliY can simultaneously interact with FlhG, strongly argues for a model in which FlhG confines flagella biosynthesis to regions of active cell wall biosynthesis. Thus, the FlhG-GpsB interaction appears to enable the locally restrained stimulation of the GTPase FlhF, known for its role to localize flagella in various bacterial species.","PeriodicalId":19006,"journal":{"name":"Molecular Microbiology","volume":"83 1","pages":""},"PeriodicalIF":3.6,"publicationDate":"2025-05-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144087646","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Pseudomonas syringae Socially Induced Swimming Motility Requires the Molybdenum Cofactor 丁香假单胞菌社会性诱导的游泳运动需要钼辅助因子

IF 3.6 2区生物学

Molecular Microbiology Pub Date : 2025-05-19 DOI: 10.1111/mmi.15378

Zichu Yang, Bryan Swingle

{"title":"Pseudomonas syringae Socially Induced Swimming Motility Requires the Molybdenum Cofactor","authors":"Zichu Yang, Bryan Swingle","doi":"10.1111/mmi.15378","DOIUrl":"https://doi.org/10.1111/mmi.15378","url":null,"abstract":"Social interactions among bacteria can induce behaviors that affect their fitness and influence how complex communities assemble. Here we report a new socially induced motility behavior that we refer to as baited expansion in Pseudomonas syringae pv. tomato DC3000 (Pst DC3000), a plant pathogenic bacterium. We found Pst DC3000 displayed strongly induced swimming motility towards nearby colonies of Dickeya dianthicola or Escherichia coli. We developed a controlled system to visualize and characterize the development of baited expansion. Our results provide evidence that baited expansion behavior occurs in response to a chemical gradient established and maintained by the bait colony. We also found this behavior correlated with distinct transcriptional profiles and identified molybdenum cofactor (Moco) and a Moco‐utilizing oxidoreductase as crucial factors facilitating the baited expansion behavior.","PeriodicalId":19006,"journal":{"name":"Molecular Microbiology","volume":"1 1","pages":""},"PeriodicalIF":3.6,"publicationDate":"2025-05-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144097134","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Genome-Wide Analysis of DtxR and HrrA Regulons Reveals Novel Targets and a High Level of Interconnectivity Between Iron and Heme Regulatory Networks in Corynebacterium glutamicum 谷氨酸棒状杆菌DtxR和HrrA调控子的全基因组分析揭示了铁和血红素调控网络之间的新靶点和高水平互联性

IF 3.6 2区生物学

Molecular Microbiology Pub Date : 2025-05-16 DOI: 10.1111/mmi.15376

Aileen Krüger, Ulrike Weber, Julia Frunzke

{"title":"Genome-Wide Analysis of DtxR and HrrA Regulons Reveals Novel Targets and a High Level of Interconnectivity Between Iron and Heme Regulatory Networks in Corynebacterium glutamicum","authors":"Aileen Krüger, Ulrike Weber, Julia Frunzke","doi":"10.1111/mmi.15376","DOIUrl":"https://doi.org/10.1111/mmi.15376","url":null,"abstract":"Iron is vital for most organisms, serving as a cofactor in enzymes, regulatory proteins, and respiratory cytochromes. In Corynebacterium glutamicum, iron and heme homeostasis are tightly interconnected and controlled by the global regulators DtxR and HrrA. While DtxR senses intracellular Fe2+, HrrSA is activated by heme. This study provides the first genome-wide analysis of DtxR and HrrA binding dynamics under varying iron and heme conditions using chromatin affinity purification and sequencing (ChAP-Seq). We revealed 25 novel DtxR targets and 210 previously unrecognized HrrA targets. Among these, metH, encoding homocysteine methyltransferase, and xerC, encoding a tyrosine recombinase, were bound by DtxR exclusively under heme conditions, underscoring condition-dependent variation. Activation of metH by DtxR links iron metabolism to methionine synthesis, potentially relevant for the mitigation of oxidative stress. Beyond novel targets, 16 shared targets between DtxR and HrrA, some with overlapping operator sequences, highlight their interconnected regulons. Strikingly, we demonstrate the significance of weak ChAP-Seq peaks that are often disregarded in global approaches, but feature an impact of the regulator on differential gene expression. These findings emphasize the importance of genome-wide profiling under different conditions to uncover novel targets and shed light on the complexity and dynamic nature of bacterial regulatory networks.","PeriodicalId":19006,"journal":{"name":"Molecular Microbiology","volume":"43 1","pages":""},"PeriodicalIF":3.6,"publicationDate":"2025-05-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144066349","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Heterologous Surface Display Reveals Conserved Complement Inhibition and Functional Diversification of Borrelia burgdorferi Elp Proteins 异源表面展示揭示伯氏疏螺旋体Elp蛋白的保守补体抑制和功能多样化

IF 3.6 2区生物学

Molecular Microbiology Pub Date : 2025-05-16 DOI: 10.1111/mmi.15369

Nathan Hill, Lara M. Matulina, Cameron MacIntyre, M. Amine Hassani, Sheila Thomas, Matteo Luban, Isabelle Ward, Amina Abdalla, John M. Leong, Brandon L. Garcia, Jacob E. Lemieux

{"title":"Heterologous Surface Display Reveals Conserved Complement Inhibition and Functional Diversification of Borrelia burgdorferi Elp Proteins","authors":"Nathan Hill, Lara M. Matulina, Cameron MacIntyre, M. Amine Hassani, Sheila Thomas, Matteo Luban, Isabelle Ward, Amina Abdalla, John M. Leong, Brandon L. Garcia, Jacob E. Lemieux","doi":"10.1111/mmi.15369","DOIUrl":"https://doi.org/10.1111/mmi.15369","url":null,"abstract":"Lyme disease is a tick-borne spirochetosis with diverse clinical manifestations. Genotypic and phenotypic variation among Borrelia burgdorferi strains correlates with variable manifestations of Lyme disease in humans; this diversity is attributed in part to variation in surface-exposed lipoproteins, which are targets of the human antibody response and contribute to tissue adhesion, immune evasion, and other host interactions. Many B. burgdorferi lipoproteins are encoded as multi-copy gene families, such as the OspE/F-like leader peptide (Elp) protein family, which inhibits classical complement activation by binding complement C1s. To characterize Elp allelic variants, we adapted the Pseudomonas syringae ice nucleation protein (INP) system to present B. burgdorferi lipoproteins on the surface of Escherichia coli. Using this system, we identified interactions with classical complement proteins and mapped binding regions, then validated interactions using recombinant proteins and B. burgdorferi surface display. We also discovered a novel potential interaction between Elp proteins and the mammalian basement membrane protein perlecan, thus revealing a bifunctional nature of Elps. Our findings indicate that Elps have undergone functional diversification while maintaining classical complement inhibition mediated by potent and conserved C1s binding and demonstrate that E. coli surface display offers an efficient, cost-effective, and relatively high-throughput approach to characterize B. burgdorferi lipoproteins.","PeriodicalId":19006,"journal":{"name":"Molecular Microbiology","volume":"11 1","pages":""},"PeriodicalIF":3.6,"publicationDate":"2025-05-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144066647","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

ModE Regulates Alternative Nitrogenase Expression in the Methanogen Methanosarcina acetivorans. ModE对产甲烷菌活性甲烷菌替代氮酶表达的调控。

IF 3.6 2区生物学

Molecular Microbiology Pub Date : 2025-05-12 DOI: 10.1111/mmi.15377

Melissa Chanderban,Daniel J Lessner

{"title":"ModE Regulates Alternative Nitrogenase Expression in the Methanogen Methanosarcina acetivorans.","authors":"Melissa Chanderban,Daniel J Lessner","doi":"10.1111/mmi.15377","DOIUrl":"https://doi.org/10.1111/mmi.15377","url":null,"abstract":"All methanogens that can fix nitrogen use molybdenum (Mo) nitrogenase. Some methanogens, including Methanosarcina acetivorans, also contain alternative vanadium- and iron-nitrogenases, encoded by the vnf and anf operons, respectively. These nitrogenases are produced when there is insufficient Mo to support Mo-nitrogenase activity. The factors that control the expression of the alternative nitrogenases in response to Mo availability are unknown in methanogens. Here we show that ModE is the regulator that represses transcription of the vnf and anf operons in M. acetivorans when cells are grown with Mo. CRISPRi repression of modE results in a significant increase in the transcription of the vnf and anf operons as well as the detection of Fe-nitrogenase during nitrogen fixation in the presence of Mo. Gel shift assays with recombinant ModE demonstrated that ModE binds a specific sequence motif upstream of the vnf and anf operons, as well as other genes and operons related to nitrogen fixation and Mo transport. However, purified ModE does not contain Mo, and the addition of Mo does not alter the affinity of ModE for DNA, indicating M. acetivorans ModE may not directly bind Mo. This study shows that ModE is the primary Mo-responsive regulator of alternative nitrogenase expression in M. acetivorans, but other factor(s) are likely involved in directly sensing Mo.","PeriodicalId":19006,"journal":{"name":"Molecular Microbiology","volume":"137 1","pages":""},"PeriodicalIF":3.6,"publicationDate":"2025-05-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143932897","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The Pxp Complex Detoxifies 5-Oxoproline and Promotes the Growth of Clostridioides difficile Pxp复合物解毒5-氧脯氨酸并促进艰难梭菌的生长

IF 3.6 2区生物学

Molecular Microbiology Pub Date : 2025-05-08 DOI: 10.1111/mmi.15373

Cheyenne D. Lee, Arshad Rizvi, Zavier A. Carter, Adrianne N. Edwards, Shonna M. McBride

{"title":"The Pxp Complex Detoxifies 5-Oxoproline and Promotes the Growth of Clostridioides difficile","authors":"Cheyenne D. Lee, Arshad Rizvi, Zavier A. Carter, Adrianne N. Edwards, Shonna M. McBride","doi":"10.1111/mmi.15373","DOIUrl":"https://doi.org/10.1111/mmi.15373","url":null,"abstract":"Clostridioides difficile is an anaerobic enteric pathogen that disseminates in the environment as a dormant spore. For C. difficile and other sporulating bacteria, the initiation of sporulation is a regulated process that prevents spore formation under favorable growth conditions. In Bacillus subtilis, one such mechanism for preventing sporulation is the prokaryotic 5-oxoprolinase, PxpB (KipI), which impedes the activation of the main sporulation kinase. In addition, PxpB functions as part of a complex that detoxifies the intermediate metabolite, 5-oxoproline (OP), a harmful by-product of glutamic acid and its derivatives. In this study, we investigate the orthologous Pxp proteins in C. difficile to determine their roles in the regulation of sporulation and metabolism. Through deletion of the pxpAGBC operon, we show that, unlike in B. subtilis, the Pxp (Kip) proteins have no significant impact on sporulation. However, we found that the pxp operon encodes a functional oxoprolinase that facilitates detoxification of OP. Furthermore, our data demonstrate that PxpAGBC not only detoxifies OP but also allows OP to be used as a nutrient source that supports the growth of C. difficile, thereby facilitating the conversion of a toxic by-product of metabolism into an energy source.","PeriodicalId":19006,"journal":{"name":"Molecular Microbiology","volume":"37 1","pages":""},"PeriodicalIF":3.6,"publicationDate":"2025-05-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143920960","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Luminal Phospholipase D Attacks Bacterial Membranes in Dictyostelium discoideum Phagosomes 腔内磷脂酶D攻击盘状盘牙基钢菌吞噬体中的细菌膜

IF 3.6 2区生物学

Molecular Microbiology Pub Date : 2025-05-04 DOI: 10.1111/mmi.15367

Otmane Lamrabet, Raphael Munoz-Ruiz, Imen Ayadi, Alixia Bourbon, Erwann Pain, Joseph Oddy, Pierre Cosson

引用次数: 0

The Arms Race Between Actinobacillus pleuropneumoniae and Its Genetic Environment: A Comprehensive Analysis of Its Defensome and Mobile Genetic Elements 胸膜肺炎放线杆菌与其遗传环境的军备竞赛：其防御和移动遗传因素的综合分析

IF 3.6 2区生物学

Molecular Microbiology Pub Date : 2025-05-03 DOI: 10.1111/mmi.15374

Giarlã Cunha da Silva, Ciro César Rossi

{"title":"The Arms Race Between Actinobacillus pleuropneumoniae and Its Genetic Environment: A Comprehensive Analysis of Its Defensome and Mobile Genetic Elements","authors":"Giarlã Cunha da Silva, Ciro César Rossi","doi":"10.1111/mmi.15374","DOIUrl":"https://doi.org/10.1111/mmi.15374","url":null,"abstract":"Actinobacillus pleuropneumoniae is the causative agent of pleuropneumonia in swine, a highly contagious and economically significant disease. The genetic variability of A. pleuropneumoniae complicates disease control efforts, as it enables rapid adaptation to various stressors, including antimicrobial treatments. To better understand the molecular mechanisms underlying this adaptability, we investigated the role of the bacterial defensome and its relationship with mobile genetic elements (MGEs), such as prophages, plasmids, and integrative conjugative elements (ICEs). Using bioinformatic tools, we identified a diverse and rich defensome in A. pleuropneumoniae, with an average of 16 different defense systems per strain. We found that CRISPR-Cas systems, along with other defense mechanisms, are actively involved in restricting the entry of foreign genetic material, playing a crucial role in bacterial adaptation. Additionally, we characterized several novel prophages and examined their distribution across different strains, revealing their potential contribution to the bacterium's evolutionary success. Our findings underscore the complex interplay between the bacterium's defense systems and MGEs, shedding light on how A. pleuropneumoniae maintains genetic diversity while also safeguarding itself against external threats. These insights provide a better understanding of the genetic factors that influence the pathogen's adaptability and highlight potential avenues for more effective disease control strategies.","PeriodicalId":19006,"journal":{"name":"Molecular Microbiology","volume":"109 1","pages":""},"PeriodicalIF":3.6,"publicationDate":"2025-05-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143901813","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Identification of EppR, a Second Repressor of Error-Prone DNA Polymerase Genes in Acinetobacter baumannii 鲍曼不动杆菌易出错DNA聚合酶第二抑制基因EppR的鉴定

IF 3.6 2区生物学

Molecular Microbiology Pub Date : 2025-04-19 DOI: 10.1111/mmi.15368

Brian Nguyen, Carly Ching, Ashley MacGuire, Pranav Casula, Connor Newman, Faith Finley, Veronica G. Godoy

{"title":"Identification of EppR, a Second Repressor of Error-Prone DNA Polymerase Genes in Acinetobacter baumannii","authors":"Brian Nguyen, Carly Ching, Ashley MacGuire, Pranav Casula, Connor Newman, Faith Finley, Veronica G. Godoy","doi":"10.1111/mmi.15368","DOIUrl":"https://doi.org/10.1111/mmi.15368","url":null,"abstract":"Acinetobacter baumannii is an opportunistic pathogen causing several infections that are increasingly difficult to treat due to its ability to rapidly gain antibiotic resistances. These resistances can arise due to mutations through the activity of error-prone DNA polymerases, such as DNA polymerase V (DNA Pol V) in response to DNA damage. The regulation of the DNA damage response (DDR) in A. baumannii is not completely understood; the regulation of genes encoding multiple copies of DNA Pol V is not fully characterized. Through genome-wide mutagenesis, we have identified a novel TetR-like family regulator of the umuDC and umuC genes, which we have named Error-prone polymerase regulator (EppR). We have found that EppR represses the expression of the genes encoding DNA Pol V and itself through direct binding to an EppR motif in their promoters. Lastly, we show that EppR also regulates UmuDAb, previously identified as a regulator of genes encoding DNA Pol V. These two gene products are functionally required to ensure regulation of the expression of the two umuDC, the two umuC genes as well as the regulators umuDAb and eppR genes. With these results, we propose a model in which multiple transcription factors regulate the expression of all these genes.","PeriodicalId":19006,"journal":{"name":"Molecular Microbiology","volume":"41 1","pages":""},"PeriodicalIF":3.6,"publicationDate":"2025-04-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143849527","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0