Molecular Microbiology最新文献_第8页

Molecular Microbiology of Microbiomes 微生物组的分子微生物学

IF 3.6 2区生物学

Molecular Microbiology Pub Date : 2024-09-11 DOI: 10.1111/mmi.15308

Pierre Santucci

引用次数: 0

Lactobacillus gasseri and Gardnerella vaginalis produce extracellular vesicles that contribute to the function of the vaginal microbiome and modulate host-Trichomonas vaginalis interactions. 加塞乳杆菌和阴道加德纳菌产生的细胞外囊泡有助于发挥阴道微生物群的功能，并调节宿主与阴道毛滴虫之间的相互作用。

IF 2.6 2区生物学

Molecular Microbiology Pub Date : 2024-09-01 Epub Date: 2023-07-24 DOI: 10.1111/mmi.15130

Anastasiia Artuyants, Jiwon Hong, Priscila Dauros-Singorenko, Anthony Phillips, Augusto Simoes-Barbosa

{"title":"Lactobacillus gasseri and Gardnerella vaginalis produce extracellular vesicles that contribute to the function of the vaginal microbiome and modulate host-Trichomonas vaginalis interactions.","authors":"Anastasiia Artuyants, Jiwon Hong, Priscila Dauros-Singorenko, Anthony Phillips, Augusto Simoes-Barbosa","doi":"10.1111/mmi.15130","DOIUrl":"10.1111/mmi.15130","url":null,"abstract":"Trichomonas vaginalis is an extracellular protozoan parasite of the human urogenital tract, responsible for a prevalent sexually transmitted infection. Trichomoniasis is accompanied by a dysbiotic microbiome that is characterised by the depletion of host-protective commensals such as Lactobacillus gasseri, and the flourishing of a bacterial consortium that is comparable to the one seen for bacterial vaginosis, including the founder species Gardnerella vaginalis. These two vaginal bacteria are known to have opposite effects on T. vaginalis pathogenicity. Studies on extracellular vesicles (EVs) have been focused on the direction of a microbial producer (commensal or pathogen) to a host recipient, and largely in the context of the gut microbiome. Here, taking advantage of the simplicity of the human cervicovaginal microbiome, we determined the molecular cargo of EVs produced by L. gasseri and G. vaginalis and examined how these vesicles modulate the interaction of T. vaginalis and host cells. We show that these EVs carry a specific cargo of proteins, which functions can be attributed to the opposite roles that these bacteria play in the vaginal biome. Furthermore, these bacterial EVs are delivered to host and protozoan cells, modulating host-pathogen interactions in a way that mimics the opposite effects that these bacteria have on T. vaginalis pathogenicity. This is the first study to describe side-by-side the protein composition of EVs produced by two bacteria belonging to the opposite spectrum of a microbiome and to demonstrate that these vesicles modulate the pathogenicity of a protozoan parasite. Such as in trichomoniasis, infections and dysbiosis co-occur frequently resulting in significant co-morbidities. Therefore, studies like this provide the knowledge for the development of antimicrobial therapies that aim to clear the infection while restoring a healthy microbiome.","PeriodicalId":19006,"journal":{"name":"Molecular Microbiology","volume":" ","pages":"357-371"},"PeriodicalIF":2.6,"publicationDate":"2024-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9860987","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Simvastatin induces human gut bacterial cell surface genes. 辛伐他汀诱导人类肠道细菌细胞表面基因。

IF 2.6 2区生物学

Molecular Microbiology Pub Date : 2024-09-01 Epub Date: 2023-09-15 DOI: 10.1111/mmi.15151

Veronica Escalante, Renuka R Nayak, Cecilia Noecker, Joel Babdor, Matthew Spitzer, Adam M Deutschbauer, Peter J Turnbaugh

{"title":"Simvastatin induces human gut bacterial cell surface genes.","authors":"Veronica Escalante, Renuka R Nayak, Cecilia Noecker, Joel Babdor, Matthew Spitzer, Adam M Deutschbauer, Peter J Turnbaugh","doi":"10.1111/mmi.15151","DOIUrl":"10.1111/mmi.15151","url":null,"abstract":"Drugs intended to target mammalian cells can have broad off-target effects on the human gut microbiota with potential downstream consequences for drug efficacy and side effect profiles. Yet, despite a rich literature on antibiotic resistance, we still know very little about the mechanisms through which commensal bacteria evade non-antibiotic drugs. Here, we focus on statins, one of the most prescribed drug types in the world and an essential tool in the prevention and treatment of high circulating cholesterol levels. Prior work in humans, mice, and cell culture support an off-target effect of statins on human gut bacteria; however, the genetic determinants of statin sensitivity remain unknown. We confirmed that simvastatin inhibits the growth of diverse human gut bacterial strains grown in communities and in pure cultures. Drug sensitivity varied between phyla and was dose-dependent. We selected two representative simvastatin-sensitive species for more in-depth analysis: Eggerthella lenta (phylum: Actinobacteriota) and Bacteroides thetaiotaomicron (phylum: Bacteroidota). Transcriptomics revealed that both bacterial species upregulate genes in response to simvastatin that alter the cell membrane, including fatty acid biogenesis (E. lenta) and drug efflux systems (B. thetaiotaomicron). Transposon mutagenesis identified a key efflux system in B. thetaiotaomicron that enables growth in the presence of statins. Taken together, these results emphasize the importance of the bacterial cell membrane in countering the off-target effects of host-targeted drugs. Continued mechanistic dissection of the various mechanisms through which the human gut microbiota evades drugs will be essential to understand and predict the effects of drug administration in human cohorts and the potential downstream consequences for health and disease.","PeriodicalId":19006,"journal":{"name":"Molecular Microbiology","volume":" ","pages":"372-386"},"PeriodicalIF":2.6,"publicationDate":"2024-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10940213/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10234832","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Space, the final frontier: The spatial component of phytoplankton-bacterial interactions. 空间，最后的边界：浮游植物-细菌相互作用的空间成分。

IF 2.6 2区生物学

Molecular Microbiology Pub Date : 2024-09-01 Epub Date: 2024-07-06 DOI: 10.1111/mmi.15293

Clara Martínez-Pérez, Sophie T Zweifel, Roberto Pioli, Roman Stocker

{"title":"Space, the final frontier: The spatial component of phytoplankton-bacterial interactions.","authors":"Clara Martínez-Pérez, Sophie T Zweifel, Roberto Pioli, Roman Stocker","doi":"10.1111/mmi.15293","DOIUrl":"10.1111/mmi.15293","url":null,"abstract":"Microscale interactions between marine phytoplankton and bacteria shape the microenvironment of individual cells, impacting their physiology and ultimately influencing global-scale biogeochemical processes like carbon and nutrient cycling. In dilute environments such as the ocean water column, metabolic exchange between microorganisms likely requires close proximity between partners. However, the biological strategies to achieve this physical proximity remain an understudied aspect of phytoplankton-bacterial associations. Understanding the mechanisms by which these microorganisms establish and sustain spatial relationships and the extent to which spatial proximity is necessary for interactions to occur, is critical to learning how spatial associations influence the ecology of phytoplankton and bacterial communities. Here, we provide an overview of current knowledge on the role of space in shaping interactions among ocean microorganisms, encompassing behavioural and metabolic evidence. We propose that characterising phytoplankton-bacterial interactions from a spatial perspective can contribute to a mechanistic understanding of the establishment and maintenance of these associations and, consequently, an enhanced ability to predict the impact of microscale processes on ecosystem-wide phenomena.","PeriodicalId":19006,"journal":{"name":"Molecular Microbiology","volume":" ","pages":"331-346"},"PeriodicalIF":2.6,"publicationDate":"2024-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141545048","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Neuroactive metabolites modulated by the gut microbiota in honey bees. 蜜蜂肠道微生物群对神经活性代谢物的调节作用

IF 2.6 2区生物学

Molecular Microbiology Pub Date : 2024-09-01 Epub Date: 2023-09-17 DOI: 10.1111/mmi.15167

Amélie Cabirol, Silvia Moriano-Gutierrez, Philipp Engel

引用次数: 0

From vacant to vivid: The nutritional landscape drives infant gut microbiota establishment. 从空缺到生动：营养状况推动婴儿肠道微生物群的建立。

IF 2.6 2区生物学

Molecular Microbiology Pub Date : 2024-09-01 Epub Date: 2024-07-23 DOI: 10.1111/mmi.15296

Reut Melki, Yael Litvak

引用次数: 0

Pilot study on nasal microbiota dynamics and MRSA carriage of a pig cohort housed on straw bedding. 关于使用稻草垫料饲养的猪群鼻腔微生物群动态和 MRSA 携带情况的试点研究。

IF 2.6 2区生物学

Molecular Microbiology Pub Date : 2024-09-01 Epub Date: 2023-07-31 DOI: 10.1111/mmi.15136

Natalie Effelsberg, Iris Kobusch, Hannah Schollenbruch, Sabrina Linnemann, Corinna Bang, Andre Franke, Robin Köck, Marc Boelhauve, Alexander Mellmann

{"title":"Pilot study on nasal microbiota dynamics and MRSA carriage of a pig cohort housed on straw bedding.","authors":"Natalie Effelsberg, Iris Kobusch, Hannah Schollenbruch, Sabrina Linnemann, Corinna Bang, Andre Franke, Robin Köck, Marc Boelhauve, Alexander Mellmann","doi":"10.1111/mmi.15136","DOIUrl":"10.1111/mmi.15136","url":null,"abstract":"Methicillin-resistant Staphylococcus aureus (MRSA) can be transmitted between pigs and humans on farms. Hence, the reduction of MRSA carriage in pigs could decrease the risk of zoonotic transmission. Recently, straw bedding has been found to significantly reduce MRSA carriage in pigs. The mechanisms behind this effect remain unclear but changes in the nasal microbiome may play a role. In this exploratory study, the nasal microbiota of pigs kept on straw was examined using V1/V2 16S rRNA gene sequencing. Nasal swabs were collected from 13 pigs at six different time points during the course of a full fattening cycle resulting in 74 porcine samples. In addition, straw samples were collected at each time point. Eleven out of 13 pigs were MRSA positive at housing-in. We found a strong temporal pattern in the microbial communities. Both microbial diversity and abundance of Staphylococcus species peaked in week 5 after introduction to the straw stable decreased in week 10, when all pigs turned MRSA-negative, and increased again toward the end of the fattening period. These findings show that the introduction of pigs into a new environment has a huge impact on their nasal microbiota, which might lead to unfavorable conditions for MRSA. Moreover, other Staphylococcus species may play a role in eliminating MRSA carriage. We designed a follow-up study including two different husbandry systems to further assess these effects.","PeriodicalId":19006,"journal":{"name":"Molecular Microbiology","volume":" ","pages":"403-412"},"PeriodicalIF":2.6,"publicationDate":"2024-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10286894","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Unlocking the potential of microbiome editing: A review of conjugation-based delivery. 释放微生物组编辑的潜力：基于共轭的递送综述。

IF 2.6 2区生物学

Molecular Microbiology Pub Date : 2024-09-01 Epub Date: 2023-09-02 DOI: 10.1111/mmi.15147

Pedro Dorado-Morales, Morgan Lambérioux, Didier Mazel

{"title":"Unlocking the potential of microbiome editing: A review of conjugation-based delivery.","authors":"Pedro Dorado-Morales, Morgan Lambérioux, Didier Mazel","doi":"10.1111/mmi.15147","DOIUrl":"10.1111/mmi.15147","url":null,"abstract":"In recent decades, there has been a rapid increase in the prevalence of multidrug-resistant pathogens, posing a challenge to modern antibiotic-based medicine. This has highlighted the need for novel treatments that can specifically affect the target microorganism without disturbing other co-inhabiting species, thus preventing the development of dysbiosis in treated patients. Moreover, there is a pressing demand for tools to effectively manipulate complex microbial populations. One of the approaches suggested to address both issues was to use conjugation as a tool to modify the microbiome by either editing the genome of specific bacterial species and/or the removal of certain taxonomic groups. Conjugation involves the transfer of DNA from one bacterium to another, which opens up the possibility of introducing, modifying or deleting specific genes in the recipient. In response to this proposal, there has been a significant increase in the number of studies using this method for gene delivery in bacterial populations. This MicroReview aims to provide a detailed overview on the use of conjugation for microbiome engineering, and at the same time, to initiate a discussion on the potential, limitations and possible future directions of this approach.","PeriodicalId":19006,"journal":{"name":"Molecular Microbiology","volume":" ","pages":"273-283"},"PeriodicalIF":2.6,"publicationDate":"2024-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10137854","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The optimization of microbial functions through rational environmental manipulations. 通过合理的环境操作优化微生物功能。

IF 2.6 2区生物学

Molecular Microbiology Pub Date : 2024-09-01 Epub Date: 2024-02-19 DOI: 10.1111/mmi.15236

Álvaro Sánchez, Andrea Arrabal, Magdalena San Román, Juan Díaz-Colunga

{"title":"The optimization of microbial functions through rational environmental manipulations.","authors":"Álvaro Sánchez, Andrea Arrabal, Magdalena San Román, Juan Díaz-Colunga","doi":"10.1111/mmi.15236","DOIUrl":"10.1111/mmi.15236","url":null,"abstract":"Microorganisms play a central role in biotechnology and it is key that we develop strategies to engineer and optimize their functionality. To this end, most efforts have focused on introducing genetic manipulations in microorganisms which are then grown either in monoculture or in mixed-species consortia. An alternative strategy to optimize microbial processes is to rationally engineer the environment in which microbes grow. The microbial environment is multidimensional, including factors such as temperature, pH, salinity, nutrient composition, etc. These environmental factors all influence the growth and phenotypes of microorganisms and they generally \"interact\" with one another, combining their effects in complex, non-additive ways. In this piece, we overview the origins and consequences of these \"interactions\" between environmental factors and discuss how they have been built into statistical, bottom-up predictive models of microbial function to identify optimal environmental conditions for monocultures and microbial consortia. We also overview alternative \"top-down\" approaches, such as genetic algorithms, to finding optimal combinations of environmental factors. By providing a brief summary of the state of this field, we hope to stimulate further work on the rational manipulation and optimization of the microbial environment.","PeriodicalId":19006,"journal":{"name":"Molecular Microbiology","volume":" ","pages":"294-303"},"PeriodicalIF":2.6,"publicationDate":"2024-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139900189","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

An Extracellular, Ca2+‐Activated Nuclease (EcnA) Mediates Transformation in a Naturally Competent Archaeon 细胞外 Ca2+ 激活的核酸酶（EcnA）介导自然能力古菌的转化

IF 3.6 2区生物学

Molecular Microbiology Pub Date : 2024-08-31 DOI: 10.1111/mmi.15311

Dallas R. Fonseca, Leslie A. Day, Kathryn K. Crone, Kyle C. Costa

{"title":"An Extracellular, Ca2+‐Activated Nuclease (EcnA) Mediates Transformation in a Naturally Competent Archaeon","authors":"Dallas R. Fonseca, Leslie A. Day, Kathryn K. Crone, Kyle C. Costa","doi":"10.1111/mmi.15311","DOIUrl":"https://doi.org/10.1111/mmi.15311","url":null,"abstract":"Transformation, the uptake of DNA directly from the environment, is a major driver of gene flow in microbial populations. In bacteria, DNA uptake requires a nuclease that processes dsDNA to ssDNA, which is subsequently transferred into the cell and incorporated into the genome. However, the process of DNA uptake in archaea is still unknown. Previously, we cataloged genes essential to natural transformation in Methanococcus maripaludis, but few homologs of bacterial transformation‐associated genes were identified. Here, we characterize one gene, MMJJ_16440 (named here as ecnA), to be an extracellular nuclease. We show that EcnA is Ca2+‐activated, present on the cell surface, and essential for transformation. While EcnA can degrade several forms of DNA, the highest activity was observed with ssDNA as a substrate. Activity was also observed with circular dsDNA, suggesting that EcnA is an endonuclease. This is the first biochemical characterization of a transformation‐associated protein in a member of the archaeal domain and suggests that both archaeal and bacterial transformation initiate in an analogous fashion.","PeriodicalId":19006,"journal":{"name":"Molecular Microbiology","volume":"54 1","pages":""},"PeriodicalIF":3.6,"publicationDate":"2024-08-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142101034","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0