Molecular Microbiology最新文献_第8页

The immune interactions of gut glycans and microbiota in health and disease 肠道糖和微生物群在健康和疾病中的免疫相互作用

IF 3.6 2区生物学

Molecular Microbiology Pub Date : 2024-05-04 DOI: 10.1111/mmi.15267

Mahmut Demirturk, Mukaddes Sena Cinar, Fikri Y. Avci

引用次数: 0

The binding affinity-dependent inhibition of cell growth and viability by DNA sulfur-binding domains. DNA 硫结合结构域对细胞生长和活力的抑制作用与结合亲和力有关。

IF 3.6 2区生物学

Molecular Microbiology Pub Date : 2024-05-01 Epub Date: 2024-03-13 DOI: 10.1111/mmi.15249

Yuli Wang, Fulin Ge, Jinling Liu, Wenyue Hu, Guang Liu, Zixin Deng, Xinyi He

{"title":"The binding affinity-dependent inhibition of cell growth and viability by DNA sulfur-binding domains.","authors":"Yuli Wang, Fulin Ge, Jinling Liu, Wenyue Hu, Guang Liu, Zixin Deng, Xinyi He","doi":"10.1111/mmi.15249","DOIUrl":"10.1111/mmi.15249","url":null,"abstract":"Increasing evidence suggests that DNA phosphorothioate (PT) modification serves several purposes in the bacterial host, and some restriction enzymes specifically target PT-DNA. PT-dependent restriction enzymes (PDREs) bind PT-DNA through their DNA sulfur binding domain (SBD) with dissociation constants (KD) of 5 nM~1 μM. Here, we report that SprMcrA, a PDRE, failed to dissociate from PT-DNA after cleavage due to high binding affinity, resulting in low DNA cleavage efficiency. Expression of SBDs in Escherichia coli cells with PT modification induced a drastic loss of cell viability at 25°C when both DNA strands of a PT site were bound, with one SBD on each DNA strand. However, at this temperature, SBD binding to only one PT DNA strand elicited a severe growth lag rather than lethality. This cell growth inhibition phenotype was alleviated by raising the growth temperature. An in vitro assay mimicking DNA replication and RNA transcription demonstrated that the bound SBD hindered the synthesis of new DNA and RNA when using PT-DNA as the template. Our findings suggest that DNA modification-targeting proteins might regulate cellular processes involved in DNA metabolism in addition to being components of restriction-modification systems and epigenetic readers.","PeriodicalId":19006,"journal":{"name":"Molecular Microbiology","volume":" ","pages":"971-983"},"PeriodicalIF":3.6,"publicationDate":"2024-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140120166","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Involvement of Escherichia coli YbeX/CorC in ribosomal metabolism. 大肠杆菌 YbeX/CorC 参与核糖体代谢的情况

IF 2.6 2区生物学

Molecular Microbiology Pub Date : 2024-05-01 Epub Date: 2024-03-17 DOI: 10.1111/mmi.15248

İsmail Sarıgül, Amata Žukova, Emel Alparslan, Sille Remm, Margus Pihlak, Niilo Kaldalu, Tanel Tenson, Ülo Maiväli

引用次数: 0

Bacteriophage lambda site-specific recombination. 噬菌体λ位点特异性重组。

IF 3.6 2区生物学

Molecular Microbiology Pub Date : 2024-05-01 Epub Date: 2024-02-19 DOI: 10.1111/mmi.15241

Gregory D Van Duyne, Arthur Landy

引用次数: 0

In Staphylococcus aureus, the acyl-CoA synthetase MbcS supports branched-chain fatty acid synthesis from carboxylic acid and aldehyde precursors. 在金黄色葡萄球菌中，酰基-CoA 合成酶 MbcS 支持从羧酸和醛前体合成支链脂肪酸。

IF 2.6 2区生物学

Molecular Microbiology Pub Date : 2024-05-01 Epub Date: 2024-02-16 DOI: 10.1111/mmi.15237

Marcelle C Dos Santos Ferreira, Augustus Pendleton, Won-Sik Yeo, Fabiana C Málaga Gadea, Danna Camelo, Maeve McGuire, Shaun R Brinsmade

{"title":"In Staphylococcus aureus, the acyl-CoA synthetase MbcS supports branched-chain fatty acid synthesis from carboxylic acid and aldehyde precursors.","authors":"Marcelle C Dos Santos Ferreira, Augustus Pendleton, Won-Sik Yeo, Fabiana C Málaga Gadea, Danna Camelo, Maeve McGuire, Shaun R Brinsmade","doi":"10.1111/mmi.15237","DOIUrl":"10.1111/mmi.15237","url":null,"abstract":"In the human pathogen Staphylococcus aureus, branched-chain fatty acids (BCFAs) are the most abundant fatty acids in membrane phospholipids. Strains deficient for BCFAs synthesis experience auxotrophy in laboratory culture and attenuated virulence during infection. Furthermore, the membrane of S. aureus is among the main targets for antibiotic therapy. Therefore, determining the mechanisms involved in BCFAs synthesis is critical to manage S. aureus infections. Here, we report that the overexpression of SAUSA300_2542 (annotated to encode an acyl-CoA synthetase) restores BCFAs synthesis in strains lacking the canonical biosynthetic pathway catalyzed by the branched-chain α-keto acid dehydrogenase (BKDH) complex. We demonstrate that the acyl-CoA synthetase activity of MbcS activates branched-chain carboxylic acids (BCCAs), and is required by S. aureus to utilize the isoleucine derivative 2-methylbutyraldehyde to restore BCFAs synthesis in S. aureus. Based on the ability of some staphylococci to convert branched-chain aldehydes into their respective BCCAs and our findings demonstrating that branched-chain aldehydes are in fact BCFAs precursors, we propose that MbcS promotes the scavenging of exogenous BCCAs and mediates BCFA synthesis via a de novo alternative pathway.","PeriodicalId":19006,"journal":{"name":"Molecular Microbiology","volume":" ","pages":"865-881"},"PeriodicalIF":2.6,"publicationDate":"2024-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11167679/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139747070","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Histidine kinase-mediated cross-regulation of the vancomycin-resistance operon in Clostridioides difficile 组氨酸激酶介导的难辨梭状芽孢杆菌抗万古霉素操作子的交叉调节

IF 3.6 2区生物学

Molecular Microbiology Pub Date : 2024-05-01 DOI: 10.1111/mmi.15273

Boris R. Belitsky

{"title":"Histidine kinase-mediated cross-regulation of the vancomycin-resistance operon in Clostridioides difficile","authors":"Boris R. Belitsky","doi":"10.1111/mmi.15273","DOIUrl":"https://doi.org/10.1111/mmi.15273","url":null,"abstract":"The dipeptide D-Ala-D-Ala is an essential component of peptidoglycan and the target of vancomycin. Most Clostridioides difficile strains possess the vanG operon responsible for the synthesis of D-Ala-D-Ser, which can replace D-Ala-D-Ala in peptidoglycan. The C. difficile vanG operon is regulated by a two-component system, VanRS, but is not induced sufficiently by vancomycin to confer resistance to this antibiotic. Surprisingly, in the absence of the VanS histidine kinase (HK), the vanG operon is still induced by vancomycin and also by another antibiotic, ramoplanin, in a VanR-dependent manner. This suggested the cross-regulation of VanR by another HK or kinases that are activated in the presence of certain lipid II-targeting antibiotics. We identified these HKs as CD35990 and CD22880. However, mutations in either or both HKs did not affect the regulation of the vanG operon in wild-type cells suggesting that intact VanS prevents the cross-activation of VanR by non-cognate HKs. Overproduction of VanR in the absence of VanS, CD35990, and CD22880 led to high expression of the vanG operon indicating that VanR can potentially utilize at least one more phosphate donor for its activation. Candidate targets of CD35990- and CD22880-mediated regulation in the presence of vancomycin or ramoplanin were identified by RNA-Seq.","PeriodicalId":19006,"journal":{"name":"Molecular Microbiology","volume":"198 1","pages":""},"PeriodicalIF":3.6,"publicationDate":"2024-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140817487","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The regulatory network comprising ArcAB-RpoS-RssB influences motility in Vibrio cholerae. 由 ArcAB-RpoS-RssB 组成的调控网络影响霍乱弧菌的运动能力。

IF 2.6 2区生物学

Molecular Microbiology Pub Date : 2024-05-01 Epub Date: 2024-02-07 DOI: 10.1111/mmi.15235

Martina Wölflingseder, Vera H Fengler, Verena Standhartinger, Gabriel E Wagner, Joachim Reidl

{"title":"The regulatory network comprising ArcAB-RpoS-RssB influences motility in Vibrio cholerae.","authors":"Martina Wölflingseder, Vera H Fengler, Verena Standhartinger, Gabriel E Wagner, Joachim Reidl","doi":"10.1111/mmi.15235","DOIUrl":"10.1111/mmi.15235","url":null,"abstract":"The diarrheal disease cholera is caused by the versatile and responsive bacterium Vibrio cholerae, which is capable of adapting to environmental changes. Among others, the alternative sigma factor RpoS activates response pathways, including regulation of motility- and chemotaxis-related genes under nutrient-poor conditions in V. cholerae. Although RpoS has been well characterised, links between RpoS and other regulatory networks remain unclear. In this study, we identified the ArcAB two-component system to control rpoS transcription and RpoS protein stability in V. cholerae. In a manner similar to that seen in Escherichia coli, the ArcB kinase not only activates the response regulator ArcA but also RssB, the anti-sigma factor of RpoS. Our results demonstrated that, in V. cholerae, RssB is phosphorylated by ArcB, which subsequently activates RpoS proteolysis. Furthermore, ArcA acts as a repressor of rpoS transcription. Additionally, we determined that the cysteine residue at position 180 of ArcB is crucial for signal recognition and activity. Thus, our findings provide evidence linking RpoS response to the anoxic redox control system ArcAB in V. cholerae.","PeriodicalId":19006,"journal":{"name":"Molecular Microbiology","volume":" ","pages":"850-864"},"PeriodicalIF":2.6,"publicationDate":"2024-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139697933","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Stearoyl-CoA desaturase regulates organelle biogenesis and hepatic merozoite formation in Plasmodium berghei. 硬脂酰-CoA 去饱和酶调控疟原虫细胞器的生物发生和肝丝虫的形成

IF 2.6 2区生物学

Molecular Microbiology Pub Date : 2024-05-01 Epub Date: 2024-02-28 DOI: 10.1111/mmi.15246

Sunil Kumar Narwal, Akancha Mishra, Raksha Devi, Ankit Ghosh, Hadi Hasan Choudhary, Satish Mishra

{"title":"Stearoyl-CoA desaturase regulates organelle biogenesis and hepatic merozoite formation in Plasmodium berghei.","authors":"Sunil Kumar Narwal, Akancha Mishra, Raksha Devi, Ankit Ghosh, Hadi Hasan Choudhary, Satish Mishra","doi":"10.1111/mmi.15246","DOIUrl":"10.1111/mmi.15246","url":null,"abstract":"Plasmodium is an obligate intracellular parasite that requires intense lipid synthesis for membrane biogenesis and survival. One of the principal membrane components is oleic acid, which is needed to maintain the membrane's biophysical properties and fluidity. The malaria parasite can modify fatty acids, and stearoyl-CoA Δ9-desaturase (Scd) is an enzyme that catalyzes the synthesis of oleic acid by desaturation of stearic acid. Scd is dispensable in P. falciparum blood stages; however, its role in mosquito and liver stages remains unknown. We show that P. berghei Scd localizes to the ER in the blood and liver stages. Disruption of Scd in the rodent malaria parasite P. berghei did not affect parasite blood stage propagation, mosquito stage development, or early liver-stage development. However, when Scd KO sporozoites were inoculated intravenously or by mosquito bite into mice, they failed to initiate blood-stage infection. Immunofluorescence analysis revealed that organelle biogenesis was impaired and merozoite formation was abolished, which initiates blood-stage infections. Genetic complementation of the KO parasites restored merozoite formation to a level similar to that of WT parasites. Mice immunized with Scd KO sporozoites confer long-lasting sterile protection against infectious sporozoite challenge. Thus, the Scd KO parasite is an appealing candidate for inducing protective pre-erythrocytic immunity and hence its utility as a GAP.","PeriodicalId":19006,"journal":{"name":"Molecular Microbiology","volume":" ","pages":"940-953"},"PeriodicalIF":2.6,"publicationDate":"2024-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139990715","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Live to fight another day: The bacterial nucleoid under stress 为新的一天而战压力下的细菌核仁

IF 3.6 2区生物学

Molecular Microbiology Pub Date : 2024-05-01 DOI: 10.1111/mmi.15272

Azra M. Walker, Elio A. Abbondanzieri, Anne S. Meyer

引用次数: 0

Regulation of late-acting operons by three transcription factors and a CRISPR-Cas component during Myxococcus xanthus development. 黄肉球菌发育过程中三个转录因子和一个 CRISPR-Cas 组件对晚期作用操作子的调控。

IF 3.6 2区生物学

Molecular Microbiology Pub Date : 2024-05-01 Epub Date: 2024-03-25 DOI: 10.1111/mmi.15252

Shreya Saha, Lee Kroos

{"title":"Regulation of late-acting operons by three transcription factors and a CRISPR-Cas component during Myxococcus xanthus development.","authors":"Shreya Saha, Lee Kroos","doi":"10.1111/mmi.15252","DOIUrl":"10.1111/mmi.15252","url":null,"abstract":"Upon starvation, rod-shaped Myxococcus xanthus bacteria form mounds and then differentiate into round, stress-resistant spores. Little is known about the regulation of late-acting operons important for spore formation. C-signaling has been proposed to activate FruA, which binds DNA cooperatively with MrpC to stimulate transcription of developmental genes. We report that this model can explain regulation of the fadIJ operon involved in spore metabolism, but not that of the spore coat biogenesis operons exoA-I, exoL-P, and nfsA-H. Rather, a mutation in fruA increased the transcript levels from these operons early in development, suggesting negative regulation by FruA, and a mutation in mrpC affected transcript levels from each operon differently. FruA bound to all four promoter regions in vitro, but strikingly each promoter region was unique in terms of whether or not MrpC and/or the DNA-binding domain of Nla6 bound, and in terms of cooperative binding. Furthermore, the DevI component of a CRISPR-Cas system is a negative regulator of all four operons, based on transcript measurements. Our results demonstrate complex regulation of sporulation genes by three transcription factors and a CRISPR-Cas component, which we propose produces spores suited to withstand starvation and environmental insults.","PeriodicalId":19006,"journal":{"name":"Molecular Microbiology","volume":" ","pages":"1002-1020"},"PeriodicalIF":3.6,"publicationDate":"2024-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140207297","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0