Environmental Control of Queuosine Levels in Streptococcus mutanstRNAs

IF 2.6 2区生物学 Q3 BIOCHEMISTRY & MOLECULAR BIOLOGY

Molecular Microbiology Pub Date : 2024-12-25 DOI:10.1111/mmi.15336

Marshall Jaroch, Kathryn Savage, Paul Kuipers, Jo Marie Bacusmo, Jennifer Hu, Jingjing Sun, Peter C. Dedon, Kelly C. Rice, Valérie de Crécy‐Lagard

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Abstract

Queuosine (Q) is a modification of the wobble base in tRNAs that decode NA(C/U) codons. It is ubiquitous in bacteria, including many pathogens. Streptococcus mutans is an early colonizer of dental plaque biofilm and a key player in dental caries. Using a combination of genetic and physiological approaches, the predicted Q synthesis and salvage pathways were validated in this organism. These experiments confirmed that S. mutans can synthesize Q de novo through similar pathways found in Bacillus subtilis and Escherichia coli. However, S. mutans has a distinct salvage pathway compared to these model organisms, as it uses a transporter belonging to the energy coupling factor (ECF) family controlled by a preQ1‐dependent riboswitch. Furthermore, Q levels in this oral pathogen depended heavily on the media composition, suggesting that micronutrients can affect Q‐mediated translation efficiency.

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变形链球菌strnas中排队苷水平的环境控制

Queuosine (Q)是trna中对NA（C/U）密码子进行解码的摆动碱基的修饰。它在细菌中无处不在，包括许多病原体。变形链球菌是牙菌斑生物膜的早期定植者，是龋病的重要参与者。利用遗传和生理方法的结合，预测的Q合成和回收途径在该生物体中得到验证。这些实验证实，变形链球菌可以通过与枯草芽孢杆菌和大肠杆菌相似的途径合成Q de novo。然而，与这些模式生物相比，变形链球菌具有独特的挽救途径，因为它使用属于能量偶联因子（ECF）家族的转运体，该转运体由preQ1依赖的核糖开关控制。此外，这种口腔病原体的Q水平很大程度上取决于培养基的组成，这表明微量营养素可以影响Q介导的翻译效率。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Molecular Microbiology 生物-生化与分子生物学

CiteScore

7.20

自引率

5.60%

发文量

132

审稿时长

1.7 months

期刊介绍： Molecular Microbiology, the leading primary journal in the microbial sciences, publishes molecular studies of Bacteria, Archaea, eukaryotic microorganisms, and their viruses. Research papers should lead to a deeper understanding of the molecular principles underlying basic physiological processes or mechanisms. Appropriate topics include gene expression and regulation, pathogenicity and virulence, physiology and metabolism, synthesis of macromolecules (proteins, nucleic acids, lipids, polysaccharides, etc), cell biology and subcellular organization, membrane biogenesis and function, traffic and transport, cell-cell communication and signalling pathways, evolution and gene transfer. Articles focused on host responses (cellular or immunological) to pathogens or on microbial ecology should be directed to our sister journals Cellular Microbiology and Environmental Microbiology, respectively.