Journal of Diabetes Investigation最新文献

Letter to the Editor in response to "Predictive value of biliverdin reductase-A and homeostasis model assessment of insulin resistance on mild cognitive impairment in patients with type 2 diabetes". 针对“胆绿素还原酶- a和稳态模型评估胰岛素抵抗对2型糖尿病患者轻度认知障碍的预测价值”致编辑的回复信。

IF 3.2 3区医学

Journal of Diabetes Investigation Pub Date : 2025-06-13 DOI: 10.1111/jdi.70104

Jie Chen, Li Zhou

引用次数: 0

Comment on "Concomitant use of metformin and proton pump inhibitors increases vitamin B12 deficiency risk in type 2 diabetes". 评论“同时使用二甲双胍和质子泵抑制剂会增加2型糖尿病患者维生素B12缺乏的风险”。

IF 3.2 3区医学

Journal of Diabetes Investigation Pub Date : 2025-06-13 DOI: 10.1111/jdi.70103

Kuan-Fu Liao, Shih-Wei Lai

引用次数: 0

Imeglimin improves hyperglycemia and hypoglycemia-induced cell death and mitochondrial dysfunction in immortalized adult mouse Schwann IMS32 cells. 依米霉素改善永活成年小鼠雪旺IMS32细胞高血糖和低血糖诱导的细胞死亡和线粒体功能障碍。

IF 3.2 3区医学

Journal of Diabetes Investigation Pub Date : 2025-06-13 DOI: 10.1111/jdi.70092

Ayako Kato, Wataru Nihei, Hideji Yako, Yasuaki Tatsumi, Tatsuhito Himeno, Masaki Kondo, Yoshiro Kato, Jiro Nakamura, Hideki Kamiya, Kazunori Sango, Koichi Kato

{"title":"Imeglimin improves hyperglycemia and hypoglycemia-induced cell death and mitochondrial dysfunction in immortalized adult mouse Schwann IMS32 cells.","authors":"Ayako Kato, Wataru Nihei, Hideji Yako, Yasuaki Tatsumi, Tatsuhito Himeno, Masaki Kondo, Yoshiro Kato, Jiro Nakamura, Hideki Kamiya, Kazunori Sango, Koichi Kato","doi":"10.1111/jdi.70092","DOIUrl":"https://doi.org/10.1111/jdi.70092","url":null,"abstract":"Aims/introduction: Imeglimin, a novel oral antidiabetic drug, enhances glucose-stimulated insulin secretion, improves insulin sensitivity, and reduces mitochondrial reactive oxygen species (ROS) generation. Diabetic neuropathy is driven by oxidative stress caused by hyperglycemia, with mitochondrial ROS overproduction playing a central role. Hypoglycemia also contributes to oxidative stress. This study evaluates the effects of imeglimin on Schwann cells under high- and low-glucose conditions.Materials and methods: We used IMS32 cells, an immortalized mouse Schwann cell line, to investigate cell survival and mitochondrial function under normal, high-, and low-glucose conditions. Assessments included mitochondrial oxidative stress, cytochrome c release, mitochondrial membrane potential, oxygen consumption rate (OCR), Complex I activity, and ATP synthesis.Results: High- and low-glucose conditions caused cell death, elevated mitochondrial ROS, triggered cytochrome c release, disrupted mitochondrial membrane potential, and increased OCR and Complex I activity, while suppressing ATP synthesis. Imeglimin treatment mitigated cell death, reduced oxidative stress, restored mitochondrial membrane potential, normalized OCR and Complex I activity, and improved ATP synthesis under both glucose conditions.Conclusions: Fluctuations in glucose levels impair mitochondrial function in Schwann cells, contributing to peripheral nerve damage in diabetic neuropathy. Imeglimin demonstrated protective effects by alleviating mitochondrial dysfunction and preventing apoptosis signaling. These findings suggest the potential application of imeglimin in preventing and treating diabetic neuropathy; however, the clinical implications require further investigation.","PeriodicalId":190,"journal":{"name":"Journal of Diabetes Investigation","volume":" ","pages":""},"PeriodicalIF":3.2,"publicationDate":"2025-06-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144281825","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Association of lean metabolic dysfunction-associated steatotic liver disease with carotid plaque progression in patients with type 2 diabetes mellitus. 2型糖尿病患者瘦代谢功能障碍相关脂肪变性肝病与颈动脉斑块进展的关系

IF 3.2 3区医学

Journal of Diabetes Investigation Pub Date : 2025-06-12 DOI: 10.1111/jdi.70050

Youngjoon Kim, Yongin Cho, Yong-Ho Lee, Da Hea Seo, Seong Hee Ahn, Seongbin Hong, So Hun Kim

{"title":"Association of lean metabolic dysfunction-associated steatotic liver disease with carotid plaque progression in patients with type 2 diabetes mellitus.","authors":"Youngjoon Kim, Yongin Cho, Yong-Ho Lee, Da Hea Seo, Seong Hee Ahn, Seongbin Hong, So Hun Kim","doi":"10.1111/jdi.70050","DOIUrl":"https://doi.org/10.1111/jdi.70050","url":null,"abstract":"Introduction: We aimed to investigate whether lean metabolic dysfunction-associated steatotic liver disease (MASLD) is associated with the progression of carotid atherosclerosis in patients with type 2 diabetes mellitus.Materials and methods: We investigated 828 patients with type 2 diabetes mellitus who underwent baseline abdominal and carotid artery ultrasonography, followed by repeat carotid ultrasonography after 6-8 years. MASLD was confirmed by abdominal ultrasonography, and lean body mass was defined as a body mass index <23 kg/m2. Carotid atherosclerosis progression was defined as the appearance of new carotid plaque lesions on repeat ultrasonography. The association between lean MASLD, non-lean MASLD, and the progression of carotid atherosclerosis was investigated.Results: Among the 828 patients, 57 (6.9%), 197 (23.8%), 397 (47.9%), and 177 (21.4%) were classified as lean MASLD, lean without MASLD, non-lean MASLD, and non-lean without MASLD, respectively. After 6-8 years, both lean MASLD (adjusted odds ratio [aOR], 2.57; P = 0.005) and non-lean MASLD (aOR, 1.59; P = 0.029) had a higher risk of atherosclerosis progression than controls (lean without MASLD). No significant difference was observed in the risk of atherosclerosis progression between the lean and non-lean MASLD groups.Discussion: In patients with type 2 diabetes mellitus, both lean and non-lean MASLD were significantly associated with an increased risk of carotid atherosclerosis progression compared to those who were lean without MASLD. This suggests that evaluation of liver steatosis in patients with type 2 diabetes mellitus, regardless of body weight, may help identify individuals at high risk for carotid atherosclerosis progression.","PeriodicalId":190,"journal":{"name":"Journal of Diabetes Investigation","volume":" ","pages":""},"PeriodicalIF":3.2,"publicationDate":"2025-06-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144273790","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Circulating cell-free mitochondrial DNA in diabetes mellitus: Current insights and unexplored frontiers. 糖尿病循环无细胞线粒体DNA：当前的见解和未开发的前沿。

IF 3.2 3区医学

Journal of Diabetes Investigation Pub Date : 2025-06-12 DOI: 10.1111/jdi.70071

Mohammadreza Akbari, Fatemeh Masjedi, Mohammad Nekooeian, Mahintaj Dara, Jamshid Roozbeh

{"title":"Circulating cell-free mitochondrial DNA in diabetes mellitus: Current insights and unexplored frontiers.","authors":"Mohammadreza Akbari, Fatemeh Masjedi, Mohammad Nekooeian, Mahintaj Dara, Jamshid Roozbeh","doi":"10.1111/jdi.70071","DOIUrl":"https://doi.org/10.1111/jdi.70071","url":null,"abstract":"The role and significance of circulating cell-free mitochondrial DNA (ccf-mtDNA)-small fragments of mitochondrial DNA present in plasma-are becoming increasingly clear in various diseases. The release of mtDNA into the plasma results from mitochondrial dysfunction, cell death, and NETosis, all of which are elevated in diabetes and its complications. Due to its resemblance to pathogenic DNA, mitochondrial DNA can activate several immunological pathways, leading to inflammation-a key factor in diabetes and its complications. Studies reveal a significant overlap between the immunological pathways involved in diabetes and those triggered by ccf-mtDNA. Thus, ccf-mtDNA may play a crucial role in the pathophysiology of diabetes and its complications, making it a potential diagnostic, prognostic, and therapeutic target. This review aims to summarize research findings on the role of ccf-mtDNA in diabetes, including gestational diabetes and related complications. Most studies have focused on type 2 diabetes, whereas research on ccf-mtDNA in other forms, including type 1 diabetes, remains limited. By synthesizing these insights, we seek to clarify the role of ccf-mtDNA in diabetes and identify gaps for future research.","PeriodicalId":190,"journal":{"name":"Journal of Diabetes Investigation","volume":" ","pages":""},"PeriodicalIF":3.2,"publicationDate":"2025-06-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144273791","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Diabetes and women's health. 糖尿病与妇女健康。

IF 3.2 3区医学

Journal of Diabetes Investigation Pub Date : 2025-06-10 DOI: 10.1111/jdi.70095

Ronald Cw Ma, Edith Wk Chow, Noel Yh Ng, Takashi Sugiyama, Yuzhi Deng, Wing Hung Tam, So Ling Lau, Liona C Poon, Cuilin Zhang, Usha Sriram

{"title":"Diabetes and women's health.","authors":"Ronald Cw Ma, Edith Wk Chow, Noel Yh Ng, Takashi Sugiyama, Yuzhi Deng, Wing Hung Tam, So Ling Lau, Liona C Poon, Cuilin Zhang, Usha Sriram","doi":"10.1111/jdi.70095","DOIUrl":"https://doi.org/10.1111/jdi.70095","url":null,"abstract":"There is increasing interest in the implementation of precision medicine in diabetes. Gender is an important determinant of health, and there are numerous characteristics of diabetes in women that can be taken into account for the implementation of precision medicine in diabetes. Furthermore, better appreciation of the unique clinical characteristics of diabetes in women, especially in relation to the lifecourse perspective of a woman's life, would greatly enhance public health efforts to address diabetes and its associated risk factors, and to improve the lives of women and the next generation. These include the unique challenges and opportunities linked with polycystic ovary syndrome, gestational diabetes, other pregnancy complications including preeclampsia, and the potential impact of these diagnoses in the offspring of affected women. In this review article, we will summarize some of these key aspects of how diabetes intersects with women's health, and how a holistic, integrative, and lifecourse approach to diabetes and women's health can have a significant impact well beyond just the benefits of achieving better glucose control for the women living with diabetes, but more importantly, toward improved prevention and control of noncommunicable diseases on a population level.","PeriodicalId":190,"journal":{"name":"Journal of Diabetes Investigation","volume":" ","pages":""},"PeriodicalIF":3.2,"publicationDate":"2025-06-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144256923","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

METTL3/YTHDF3 m⁶A axis promotes ferroptosis in diabetic kidney disease by stabilizing TfR1. METTL3/YTHDF3 m6A轴通过稳定TfR1促进糖尿病肾病铁下垂。

IF 3.2 3区医学

Journal of Diabetes Investigation Pub Date : 2025-06-10 DOI: 10.1111/jdi.70094

Jinmei Zhang, Xiaoping Zhou, Bin Wang, Yan Yin, Daihao Wei, Kun Li

{"title":"METTL3/YTHDF3 m6A axis promotes ferroptosis in diabetic kidney disease by stabilizing TfR1.","authors":"Jinmei Zhang, Xiaoping Zhou, Bin Wang, Yan Yin, Daihao Wei, Kun Li","doi":"10.1111/jdi.70094","DOIUrl":"https://doi.org/10.1111/jdi.70094","url":null,"abstract":"Objective: Diabetic kidney disease (DKD) is a common complication of diabetes. N6-Methyladenosine (m6A) modification is a widely studied epigenetic mechanism. Methyltransferase-like (METTL) 3 is a well-studied methyltransferase. This study aimed to investigate the role of METTL3 in DKD and the underlying mechanism.Methods: Thirty-five DKD patients and 28 control volunteers were recruited. Animal and cell DKD models were established. QRT-PCR and Western blot were performed to analyze the expression of METTL3 and fibrosis-related indicators. Cell viability and proliferation were assessed via a cell counting kit-8 and colony formation assays. Ferrous iron (Fe2+), malonaldehyde (MDA), and glutathione (GSH) contents were measured by commercial kits. The interaction between METTL3/YTH N6-methyladenosine RNA binding protein (YTHDF)3 and transferrin receptor-1 (TfR1) was examined through RNA immunoprecipitation and dual-luciferase reporter assays.Results: Results showed that METTL3-mediated m6A modification was elevated in kidney tissues of DKD patients and in high glucose (HG)-treated human renal mesangial cells (HRMCs). Besides, HG-treated HRMCs showed increased ferroptosis. In addition, METTL3 inhibition increased cell proliferation and inhibited ferroptosis in HRMCs. Mechanically, the METTL3/YTHDF3 m6A axis enhanced the stability of TfR1 mRNA. Moreover, YTHDF3 inhibition increased cell proliferation and inhibited ferroptosis in HRMCs. Finally, in vivo study results indicated that METTL3 deficiency inhibited ferroptosis and improved pathological damages.Conclusions: In summary, METTL3/YTHDF3 m6A axis promoted ferroptosis in DKD by stabilizing TfR1, which could provide a reference for DKD treatment.","PeriodicalId":190,"journal":{"name":"Journal of Diabetes Investigation","volume":" ","pages":""},"PeriodicalIF":3.2,"publicationDate":"2025-06-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144256977","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Leptin promoter G2548A variant, elevated plasma leptin levels, and increased risk of Type 2 diabetes with CAD in Iranian patients: A genetic association study. 伊朗患者瘦素启动子G2548A变异、血浆瘦素水平升高和2型糖尿病合并冠心病风险增加：一项遗传关联研究

IF 3.2 3区医学

Journal of Diabetes Investigation Pub Date : 2025-06-10 DOI: 10.1111/jdi.70077

Leila Saremi, Nazanin Ahmadi, Fatemeh Feizy, Shirin Lotfipanah, Mohammad Ebrahim Ghaffari, Zohreh Saltanatpour

{"title":"Leptin promoter G2548A variant, elevated plasma leptin levels, and increased risk of Type 2 diabetes with CAD in Iranian patients: A genetic association study.","authors":"Leila Saremi, Nazanin Ahmadi, Fatemeh Feizy, Shirin Lotfipanah, Mohammad Ebrahim Ghaffari, Zohreh Saltanatpour","doi":"10.1111/jdi.70077","DOIUrl":"https://doi.org/10.1111/jdi.70077","url":null,"abstract":"Background: Type 2 diabetes mellitus (T2DM) is a significant risk factor for coronary artery disease (CAD). Leptin polymorphism is known to be associated with obesity and Type 2 diabetes mellitus. This study aimed to investigate the possible links between a specific leptin polymorphism (LEP 2548G/A) and plasma leptin level with the risk of Type 2 diabetes mellitus patients with CAD in the Iranian population for the first time.Methods and results: A total of 150 patients with Type 2 diabetes mellitus and CAD were included in the study, along with 150 nondiabetic controls. Blood samples were collected from participants. Biochemical analysis and genotyping were done using the polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) method. The plasma leptin level was higher in females in the case group. There was a positive correlation between females and obese diabetic subjects (P < 0.001). Regarding the single-nucleotide polymorphism in the leptin gene promoter, we found that the variant genotypes AA and GG were associated with a twofold (OR = 2.09, P = 0.006) and fourfold (OR = 4.2, P = 0.017) increased risk of Type 2 diabetes mellitus patients with CAD, respectively. The GG genotype was also associated with obesity and higher plasma leptin level in the case group (OR = 4.92, P < 0.001).Conclusions: Leptin G2548A and plasma leptin levels may contribute to Type 2 diabetes with CAD in the Iranian population.","PeriodicalId":190,"journal":{"name":"Journal of Diabetes Investigation","volume":" ","pages":""},"PeriodicalIF":3.2,"publicationDate":"2025-06-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144256924","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Blood glucose screening in dental clinics as an opportunity for detection of diabetes and prediabetes: The Kyoutou Dental and Diabetes (KDD) Study. 在牙科诊所进行血糖筛查作为检测糖尿病和前驱糖尿病的机会：弯头牙科和糖尿病（KDD）研究。

IF 3.2 3区医学

Journal of Diabetes Investigation Pub Date : 2025-06-06 DOI: 10.1111/jdi.70093

Nozomi Harai, Masato Tawata, Hiroyuki Nakamura, Takahito Fujitani, Shinji Akiyama, Tomoyuki Takagi, Kazuki Itsumura, Eri Shukuzawa, Syuuichi Saotome, Jin Mikami, Akira Ozawa, Kouki Oka, Kenji Mitsuzuka, Ai Isoyama, Minoru Nakazato, Eizou Okubo, Norihiko Yokomori, Kaoru Aida, Tadao Ooka, Kyoichiro Tsuchiya

{"title":"Blood glucose screening in dental clinics as an opportunity for detection of diabetes and prediabetes: The Kyoutou Dental and Diabetes (KDD) Study.","authors":"Nozomi Harai, Masato Tawata, Hiroyuki Nakamura, Takahito Fujitani, Shinji Akiyama, Tomoyuki Takagi, Kazuki Itsumura, Eri Shukuzawa, Syuuichi Saotome, Jin Mikami, Akira Ozawa, Kouki Oka, Kenji Mitsuzuka, Ai Isoyama, Minoru Nakazato, Eizou Okubo, Norihiko Yokomori, Kaoru Aida, Tadao Ooka, Kyoichiro Tsuchiya","doi":"10.1111/jdi.70093","DOIUrl":"https://doi.org/10.1111/jdi.70093","url":null,"abstract":"Aims/introduction: This study aimed to determine the prevalence and characteristics of positive blood glucose screening for diabetes or prediabetes among dental patients.Materials and methods: This prospective cohort study recruited 749 dental patients aged 18 years and older from October 1, 2023 to August 9, 2024. Patients undergoing treatment for diabetes were excluded. Blood glucose measurements were taken using fingerstick, with screening positivity defined as fasting blood glucose levels of 100 mg/dL or more or non-fasting blood glucose levels of 140 mg/dL or more. Positive individuals were referred to internal medicine clinics. The background and dental results of the positive and negative screening groups were compared, and multivariate logistic regression was performed.Results: Participants' average age was 54.5 ± 16.2 years, with 369 men (49.2%) and 19 women (2.5%) with a history of diabetes. Blood glucose screening was positive in 139 individuals (18.6%). Among the 34 patients who visited the internal medicine clinics, eight were diagnosed with diabetes and 10 with prediabetes. Patients in the positive group were significantly older, included more men, had more individuals with a history of diabetes, and exhibited a higher bleeding on probing (BOP) rate and alveolar bone loss (BL). The BOP rate and BL remained significant after adjustment.Conclusions: Blood glucose measurements in dental clinics may provide a practical and valuable opportunity for the early detection and intervention of diabetes and prediabetes.","PeriodicalId":190,"journal":{"name":"Journal of Diabetes Investigation","volume":" ","pages":""},"PeriodicalIF":3.2,"publicationDate":"2025-06-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144232786","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Tracing the molecular landscape of diabetic nephropathy: Insights from machine learning and experiment verification. 追踪糖尿病肾病的分子景观：来自机器学习和实验验证的见解。

IF 3.2 3区医学

Journal of Diabetes Investigation Pub Date : 2025-06-05 DOI: 10.1111/jdi.70026

Shahad W Kattan, Ahmed M Basri, Mohammad H Alhashmi, Amany I Almars, Reem Hasaballah Alhasani, Ifat Alsharif, Ikhlas A Sindi, Ahmad H Mufti, Iman S Abumansour, Nasser A Elhawary, Aishah Abdullah Qahtani, Hailah M Almohaimeed

{"title":"Tracing the molecular landscape of diabetic nephropathy: Insights from machine learning and experiment verification.","authors":"Shahad W Kattan, Ahmed M Basri, Mohammad H Alhashmi, Amany I Almars, Reem Hasaballah Alhasani, Ifat Alsharif, Ikhlas A Sindi, Ahmad H Mufti, Iman S Abumansour, Nasser A Elhawary, Aishah Abdullah Qahtani, Hailah M Almohaimeed","doi":"10.1111/jdi.70026","DOIUrl":"https://doi.org/10.1111/jdi.70026","url":null,"abstract":"Objective: Diabetes is a chronic disease resulting from insufficient insulin secretion or impaired insulin function. Diabetic nephropathy (DN) is one of the most common complications of diabetes and a leading cause of end-stage renal disease. Early diagnosis of DN is crucial for timely intervention and effective disease management.Methods: Gene expression profiles GSE142025 and GSE220226 were retrieved from the GEO database and combined into a metadata cohort, while GSE189007 was obtained as an independent validation dataset. Differentially expressed genes (DEGs) were identified in 46 glomerular samples from DN patients and 31 control samples. Gene Ontology (GO) and Disease Ontology (DO) enrichment analyses, gene set enrichment analysis (GSEA), least absolute shrinkage and selection operator (LASSO) regression, support vector machine-recursive feature elimination (SVM-RFE) analysis, and area under the curve (AUC) calculations were performed.Results: A total of 109 DEGs were identified. Among them, DUSP1, EGR1, FPR1, G6PC, GDF15, LOX, LPL, PRKAR2B, PTGDS, and TPPP3 were selected as potential diagnostic biomarkers for DN. These biomarkers exhibited a positive correlation with immune cell infiltration. Experimental validation identified LOX as the most promising novel diagnostic biomarker for DN. This study provides new insights into the early diagnosis, pathogenesis, and molecular mechanisms of DN.","PeriodicalId":190,"journal":{"name":"Journal of Diabetes Investigation","volume":" ","pages":""},"PeriodicalIF":3.2,"publicationDate":"2025-06-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144223858","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0