Mycorrhiza最新文献_第6页

A membrane-separated bi-compartmented root organ culture system enables scalable in vitro growth of Gigaspora sp. and Scutellospora reticulata. 膜分离的双室根器官培养系统使Gigaspora sp.和Scutellospora reticulata的离体生长可扩展。

IF 4 2区生物学

Mycorrhiza Pub Date : 2026-05-29 DOI: 10.1007/s00572-026-01273-4

Bérengère Bastogne, Nicolas Schtickzelle, Stéphane Declerck, Ismahen Lalaymia

{"title":"A membrane-separated bi-compartmented root organ culture system enables scalable in vitro growth of Gigaspora sp. and Scutellospora reticulata.","authors":"Bérengère Bastogne, Nicolas Schtickzelle, Stéphane Declerck, Ismahen Lalaymia","doi":"10.1007/s00572-026-01273-4","DOIUrl":"10.1007/s00572-026-01273-4","url":null,"abstract":"Root Organ Cultures (ROC) of arbuscular mycorrhizal fungi (AMF) have greatly advanced the study of these obligate biotrophs and provide a reliable source of clean material for inoculum production. While ROC in bi-compartmented systems are well established for Rhizophagus and Glomus species, they are rarely successful for species within the Gigasporaceae which are generally grown in mono-compartmented in vitro cultures systems. Here, we developed an innovative membrane-separated bi-compartmented root organ culture system (MSB-ROC system), in which a 15 μm nylon mesh physically separates the root compartment from the hyphal compartment. The system was compared to the classical mono-compartmented in vitro culture system using two Gigasporaceae: Gigaspora sp. MUCL 52331 and Scutellospora reticulata CNPAB11. We demonstrated that, in both systems, auxiliary cell and spore production followed sigmoidal growth patterns (lag, log and stationary phase) but reached significantly higher abundances in the MSB-ROC system. Auxiliary cell abundance increased four-fold in Gigaspora sp. and two-fold in S. reticulata, while spore abundance increased seven-fold and two-fold (i.e., a mean of 131 and 82 spores of Gigaspora sp. and S. reticulata), respectively. These results indicate that physically separating the root and hyphal compartments enhances extraradical growth and sporulation, making the MSB-ROC system an efficient tool for non-destructive monitoring of fungal development, ecological and physiological studies, spore production, and related molecular studies.","PeriodicalId":18965,"journal":{"name":"Mycorrhiza","volume":"36 3","pages":""},"PeriodicalIF":4.0,"publicationDate":"2026-05-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148055769","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Partial mycoheterotrophy in the arbuscular mycorrhizal Gentiana squarrosa (Gentianaceae) demonstrated by coculture assays using C₃ and C₄ plants. 用C3和C4植物共培养试验证实了丛枝菌根龙胆（Gentiana squarrosa）的部分分枝异养。

IF 4 2区生物学

Mycorrhiza Pub Date : 2026-05-28 DOI: 10.1007/s00572-026-01271-6

Masahide Yamato, Moe Sasuga, Keito Shimabukuro, Ryota Kusakabe, Kenji Suetsugu

{"title":"Partial mycoheterotrophy in the arbuscular mycorrhizal Gentiana squarrosa (Gentianaceae) demonstrated by coculture assays using C3 and C4 plants.","authors":"Masahide Yamato, Moe Sasuga, Keito Shimabukuro, Ryota Kusakabe, Kenji Suetsugu","doi":"10.1007/s00572-026-01271-6","DOIUrl":"10.1007/s00572-026-01271-6","url":null,"abstract":"Partial mycoheterotrophy is a nutritional mode in which green plants supplement photosynthetically derived carbon with fungal carbon obtained from associated fungi. In green arbuscular mycorrhizal (AM) plants, however, detecting partial mycoheterotrophy using natural ¹³C abundance is challenging, because the δ¹³C signatures of AM fungi often resemble those of their host plants. To overcome this limitation, we exploited the large natural δ¹³C contrast between C₃ and C₄ plants as an intrinsic tracer and tested for the incorporation of donor-derived carbon into the shoots of Gentiana squarrosa (Gentianaceae). Using an AM fungus commonly associated with G. squarrosa, we conducted coculture experiments with either a C₃ or C₄ companion plant. A compartmentalized U-shaped coculture system was employed, in which the roots of the companion plant were separated from G. squarrosa seedlings by a nylon mesh that permitted only hyphal passage, thereby minimizing alternative carbon transfer pathways, such as indirect transfer via CO₂ released by donor root respiration. Shoot δ¹³C values of G. squarrosa were significantly higher when seedlings were connected to a C₄ companion than when connected to a C₃ companion. Furthermore, among individuals grown with a C₄ companion, shoot dry mass was strongly and positively correlated with shoot δ¹³C values. These results demonstrate the incorporation of donor-derived carbon into G. squarrosa via AM fungal networks, providing robust evidence of partial mycoheterotrophy and suggesting that the mycoheterotrophy contributed to growth in this AM-associated species.","PeriodicalId":18965,"journal":{"name":"Mycorrhiza","volume":"36 3","pages":""},"PeriodicalIF":4.0,"publicationDate":"2026-05-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13219101/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148043062","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Comparative analyses of transcriptionally regulated downstream genes between arbuscular mycorrhizal and ectomycorrhizal symbioses in the single host plant Eucalyptus grandis. 巨桉丛枝菌根与外生菌根共生体下游转录调控基因的比较分析。

IF 4 2区生物学

Mycorrhiza Pub Date : 2026-05-25 DOI: 10.1007/s00572-026-01272-5

Takaya Tominaga, Yoshiki Hayashi, Tomomi Noguchi, Hironori Kaminaka

{"title":"Comparative analyses of transcriptionally regulated downstream genes between arbuscular mycorrhizal and ectomycorrhizal symbioses in the single host plant Eucalyptus grandis.","authors":"Takaya Tominaga, Yoshiki Hayashi, Tomomi Noguchi, Hironori Kaminaka","doi":"10.1007/s00572-026-01272-5","DOIUrl":"10.1007/s00572-026-01272-5","url":null,"abstract":"In nature, some tree species interact with both arbuscular mycorrhizal (AM) Glomeromycotina fungi and ectomycorrhizal (ECM) Basidiomycota/Ascomycota fungi, and are termed dual mycorrhizal plants. Although the AM-upregulated genes and their functions have been well studied, those of ECM symbiosis remain unclear, despite their essential roles in forest ecosystems. Therefore, this study aimed to compare symbiosis-regulated downstream genes in the dual mycorrhizal model tree, Eucalyptus grandis, during fully developed AM and ECM symbioses. First, we conducted a comparative transcriptomic analysis and found a distinct transcriptional profile between E. grandis AM and ECM roots. Notably, none of the examined AM-related downstream genes were upregulated in the ECM roots. To identify ECM-specific genes and their expression patterns, comparative genomic analysis was performed. This study identified several gene families, including NAC transcription factors, that significantly expanded across the examined ECM lineages. Interestingly, we identified some ECM-promoted NAC transcription factors in the ECM roots of E. grandis, Populus trichocarpa, and Castanea mollissima. Moreover, none of the Eucalyptus NAC genes were transcriptionally promoted during AM symbiosis. Taken together, our results indicate that the downstream pathways necessary for the establishment of AM and ECM symbioses would be distinct.","PeriodicalId":18965,"journal":{"name":"Mycorrhiza","volume":"36 3","pages":""},"PeriodicalIF":4.0,"publicationDate":"2026-05-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148015988","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

AMF-induced salinity tolerance in durum wheat is associated with transcriptomic modulation of the Cation/Calcium Exchanger 1 (CCX1). amf诱导的硬粒小麦耐盐性与阳离子/钙交换器1 （CCX1）的转录组调节有关。

IF 4 2区生物学

Mycorrhiza Pub Date : 2026-05-19 DOI: 10.1007/s00572-026-01269-0

Neda Zavarshani, Mehdi Zarei, Roohollah Shamloo-Dashtpagerdi, Ali Dadkhodaie, Sedigheh Safarzadeh Shirazi

{"title":"AMF-induced salinity tolerance in durum wheat is associated with transcriptomic modulation of the Cation/Calcium Exchanger 1 (CCX1).","authors":"Neda Zavarshani, Mehdi Zarei, Roohollah Shamloo-Dashtpagerdi, Ali Dadkhodaie, Sedigheh Safarzadeh Shirazi","doi":"10.1007/s00572-026-01269-0","DOIUrl":"10.1007/s00572-026-01269-0","url":null,"abstract":"Salinity is a major abiotic stress that severely constrains durum wheat (Triticum turgidum subsp. durum) productivity. Although the beneficial effects of arbuscular mycorrhizal fungi (AMF) on plant performance under salt stress are well established, the molecular mechanisms underlying this symbiosis remain insufficiently characterized. In this study, we applied an integrated transcriptomic and network-based approach to identify regulatory components associated with AMF-mediated salt stress responses in durum wheat. Comparative RNA-Seq analysis between salinity-stressed and AMF-treated plants revealed 572 differentially expressed genes (DEGs). Network topology analysis identified the Cation/Calcium Exchanger 1 (TdCCX1) as a candidate hub gene with high centrality, suggesting a potential integrative role in ion and stress signaling pathways. Promoter analysis of TdCCX1 revealed multiple stress- and symbiosis-related cis-elements, indicating dual regulatory control. Physiological and biochemical validation in a salinity-susceptible durum wheat genotype showed that AMF inoculation, particularly with Rhizophagus irregularis, significantly improved shoot and root biomass, enhanced K⁺/Na⁺ homeostasis, increased phosphorus accumulation, and reduced oxidative damage under salinity. Notably, TdCCX1 expression was strongly induced by salt stress but significantly attenuated in AMF-colonized plants. These findings suggest that AMF colonization modulates ion-exchange-related transcriptional networks, potentially reducing the plant's reliance on energy-intensive internal transport processes. This study provides transcriptome-guided insights into AMF-host interactions under salinity and highlights TdCCX1 as a promising candidate for future functional studies.","PeriodicalId":18965,"journal":{"name":"Mycorrhiza","volume":"36 3","pages":""},"PeriodicalIF":4.0,"publicationDate":"2026-05-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147973948","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Ectomycorrhizal fungal diversity may be influenced by arbuscular mycorrhizal trees in mixed temperate forests. 温带混交林外生菌根真菌多样性可能受到丛枝菌根树的影响。

IF 4 2区生物学

Mycorrhiza Pub Date : 2026-05-11 DOI: 10.1007/s00572-026-01267-2

Andrew M Cortese, Andrew C Eagar, Sara M Moledor, Kurt A Smemo, Richard P Phillips, Christopher B Blackwood

{"title":"Ectomycorrhizal fungal diversity may be influenced by arbuscular mycorrhizal trees in mixed temperate forests.","authors":"Andrew M Cortese, Andrew C Eagar, Sara M Moledor, Kurt A Smemo, Richard P Phillips, Christopher B Blackwood","doi":"10.1007/s00572-026-01267-2","DOIUrl":"10.1007/s00572-026-01267-2","url":null,"abstract":"Mycorrhizal fungi are critical plant mutualists that influence nutrient cycling, yet we have an incomplete understanding of factors driving their diversity and function. While it's presumed that diverse host plant communities support diverse mycorrhizal fungal communities, abiotic factors and non-host plants may also be important. We investigated whether ectomycorrhizal fungal (ECMF) communities in soils and roots varied among plots differing in tree and soil properties in three mixed ectomycorrhizal (ECM)-arbuscular mycorrhizal (AM) forests in NY, USA. We used mixed-effects models and multivariate tests to investigate ECMF taxonomic and functional responses to (1) ECM tree dominance, (2) taxonomic and phylogenetic diversity of ECM and AM trees, and (3) soil properties. ECMF taxonomic diversity was most strongly related to ECM tree dominance and the diversity of AM - but not ECM - trees. ECMF functional diversity was mostly related to soil factors and to a lesser extent tree communities. In most cases, soil nitrogen negatively influenced ECMF diversity. Collectively, our results suggest non-host (i.e., AM) tree diversity is associated with increased ECMF diversity, potentially by modifying soil conditions and resource availability. As such, mixed plots dominated by trees associating with different mycorrhizal types may be hotspots for ECMF diversity, with consequences for ecosystem functioning.","PeriodicalId":18965,"journal":{"name":"Mycorrhiza","volume":"36 3","pages":""},"PeriodicalIF":4.0,"publicationDate":"2026-05-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13158259/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147868979","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Indigenous inoculant dampens the impact of remediation of heavy metal polluted soil on arbuscular mycorrhizal fungal communities. 乡土接种剂抑制重金属污染土壤修复对丛枝菌根真菌群落的影响。

IF 4 2区生物学

Mycorrhiza Pub Date : 2026-05-09 DOI: 10.1007/s00572-026-01268-1

Nataša Šibanc, Dave R Clark, Marjetka Suhadolc, Domen Leštan, Alex J Dumbrell, Irena Maček

{"title":"Indigenous inoculant dampens the impact of remediation of heavy metal polluted soil on arbuscular mycorrhizal fungal communities.","authors":"Nataša Šibanc, Dave R Clark, Marjetka Suhadolc, Domen Leštan, Alex J Dumbrell, Irena Maček","doi":"10.1007/s00572-026-01268-1","DOIUrl":"10.1007/s00572-026-01268-1","url":null,"abstract":"The finite resource of fertile soil can take thousands of years to develop. Non-degradable toxic metals (heavy metals), pose a significant health risk due to their persistence in the environment. Soil washing with EDTA (ethylenediaminetetraacetic acid) has been shown to remove toxic metals (Pb, Zn, Cd), but this process can also have a significant impact on soil microbial diversity. In this study, we investigated, using molecular methods (18S rRNA amplicon sequencing), the development of the arbuscular mycorrhizal (AM) fungal communities in the roots of Lolium perenne after a soil remediation treatment with EDTA. Plants were grown on two soil types (calcareous and acidic), in remediated and control (unremediated, polluted) soils, and with or without the addition of a local environmental inoculant (grassland soil and roots). The addition of the inoculant increased the richness and evenness of AM fungi, and reduced the compositional dissimilarity of communities between remediated and unremediated soils. Disturbance from the remediation process and inoculation, lead to a shift in the identity of dominant taxa reflecting different traits among the AM fungi. This suggested two main mechanisms behind community development in remediated soils, i.e. priority effects and competitive exclusion among dominant taxa. These findings emphasise the importance of nature-based solutions (indigenous environmental inoculants) and AM fungi for sustainable soil management practices. This is particularly important for restoring soil biodiversity in sites that have been exposed to multiple disturbances, which may include contaminated sites and soil remediation, as well as conventional agricultural systems, and urban soils.","PeriodicalId":18965,"journal":{"name":"Mycorrhiza","volume":"36 3","pages":""},"PeriodicalIF":4.0,"publicationDate":"2026-05-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13156140/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147856844","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Deciphering bacterial community composition and function at critical interfaces of plant-arbuscular mycorrhizal fungi-bacterial holobiont. 解读植物-丛枝菌根真菌-细菌全息共生体关键界面的细菌群落组成和功能。

IF 4 2区生物学

Mycorrhiza Pub Date : 2026-05-08 DOI: 10.1007/s00572-026-01265-4

Letian Wang, Jiachao Zhou, Wenjun Wei, Timothy S George, Gu Feng

{"title":"Deciphering bacterial community composition and function at critical interfaces of plant-arbuscular mycorrhizal fungi-bacterial holobiont.","authors":"Letian Wang, Jiachao Zhou, Wenjun Wei, Timothy S George, Gu Feng","doi":"10.1007/s00572-026-01265-4","DOIUrl":"10.1007/s00572-026-01265-4","url":null,"abstract":"Arbuscular mycorrhizal (AM) fungi connect plant roots and soil bacteria, forming a cross-kingdom holobiont driven by plant-derived carbon flux and soil-derived nutrient flux. This holobiont encompasses not only roots and rhizosphere but also interfaces expanded by slender AM fungal hyphae. Our understanding of the microbiomes across these interconnected interfaces remains limited and fragmented. We used a split-root microcosm to inoculate the same maize root system with three AM fungal species, establishing a simplified holobiont. Amplicon sequencing, Biolog-ECO plates, and 13CO₂ labelling revealed the bacterial diversity and community-level physiological profiles (CLPPs). Bacterial communities colonizing non-mycorrhizal roots, mycorrhizal roots, and hyphae exhibited distinct structures and CLPPs. AM fungal species significantly influenced these bacterial communities, particularly in the mycorrhizosphere and hyphosphere, where notable changes occurred in key nutrient-cycling groups, such as phosphate solubilizers and nitrogen fixers. The diversity of the hyphosphere microbiome was closely aligned with fungal phylogeny. Additionally, a stable core microbiome persisted across all interfaces within the plant-AM fungi-bacterial holobiont, with key taxa such as Pseudomonas and Bacillus harboring the P-mobilizing genes encoding quinoprotein glucose dehydrogenase (gcd) and β-propeller phytase (bpp), highlighting their functional importance in nutrient cycling. Our study provides a comprehensive and precise hyphal-scale characterization of microbial communities across key interfaces, offering detailed insights into plant-microbial dynamics driving nutrient cycling and ecosystem functioning.","PeriodicalId":18965,"journal":{"name":"Mycorrhiza","volume":"36 3","pages":""},"PeriodicalIF":4.0,"publicationDate":"2026-05-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147840291","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Root-derived AMF communities modulate growth and nutrient dynamics in grapevine rootstocks. 根源AMF群落调节葡萄砧木生长和营养动态。

IF 4 2区生物学

Mycorrhiza Pub Date : 2026-05-07 DOI: 10.1007/s00572-026-01264-5

Romy Moukarzel, Hayley J Ridgway, Lauren Waller, Alexis Guerin-Laguette, Natalia Cripps-Guazzone, E Eirian Jones

{"title":"Root-derived AMF communities modulate growth and nutrient dynamics in grapevine rootstocks.","authors":"Romy Moukarzel, Hayley J Ridgway, Lauren Waller, Alexis Guerin-Laguette, Natalia Cripps-Guazzone, E Eirian Jones","doi":"10.1007/s00572-026-01264-5","DOIUrl":"10.1007/s00572-026-01264-5","url":null,"abstract":"Arbuscular mycorrhizal fungi (AMF) play vital roles in sustainable agriculture, yet evidence linking AMF community composition to plant benefits remains limited. To address this gap, we inoculated two commercial rootstocks (Schwarzmann and 5 C) with AMF communities recovered from different rootstocks from one site to determine effects on plant growth parameters and physiological responses. A glasshouse experiment using a 'home' and 'away' approach was designed to examine the interaction between rootstock variety and different AMF communities, including those from their own ('home') and other rootstocks' rhizosphere soils ('away'). Our results showed that rootstocks grown in their 'home' AMF communities exhibited greater above and below ground biomass compared to 'away' AMF communities, highlighting rootstock specificity in selecting AMF communities. AMF communities increased chlorophyll content and nutrient uptake (copper, boron) in grapevine leaves, where AMF communities dominated by Funneliformis sp., Ambispora sp. followed by Glomus spp. were associated with enhanced grapevine growth. This study enhances our understanding of community-level AMF-grapevine interactions and highlight the ecosystem services these fungi provide. Future research is needed using grafted plants to evaluate their response with different scions following AMF inoculation and to assess the effects of these AMF communities on berry biochemical composition.","PeriodicalId":18965,"journal":{"name":"Mycorrhiza","volume":"36 3","pages":""},"PeriodicalIF":4.0,"publicationDate":"2026-05-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13152886/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147840251","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Terfezia claveryi MAT locus characterization uncovers evolutionary insights about sexual reproduction of Pezizomycetes and reveals mating type dynamics in mycorrhizal plants. 特费齐亚（Terfezia claveryi） MAT位点的特征揭示了特费齐亚菌有性生殖的进化见解，揭示了菌根植物的交配类型动态。

IF 4 2区生物学

Mycorrhiza Pub Date : 2026-05-07 DOI: 10.1007/s00572-026-01266-3

Laura Andreu-Ardil, Ángel L Guarnizo, Alfonso Navarro-Ródenas, Francisco Arenas, Manuela Pérez-Gilabert, José Eduardo Marqués-Gálvez, Francesco Paolocci, Asunción Morte

{"title":"Terfezia claveryi MAT locus characterization uncovers evolutionary insights about sexual reproduction of Pezizomycetes and reveals mating type dynamics in mycorrhizal plants.","authors":"Laura Andreu-Ardil, Ángel L Guarnizo, Alfonso Navarro-Ródenas, Francisco Arenas, Manuela Pérez-Gilabert, José Eduardo Marqués-Gálvez, Francesco Paolocci, Asunción Morte","doi":"10.1007/s00572-026-01266-3","DOIUrl":"10.1007/s00572-026-01266-3","url":null,"abstract":"Terfezia claveryi is a hypogeous fungus that forms desert truffles through ectendomycorrhizal symbiosis with Cistaceae plants in arid and semiarid environments. The study presented herein elucidates the organization and structure of the mating type (MAT) locus in this species and the spatio-temporal dynamics of T. claveryi strains in Helianthemum almeriense mycorrhizal plants and soil from nursery to field. MAT genes are the master loci controlling sexual reproduction and development in fungi. Our findings demonstrate that T. claveryi is a haploid and heterothallic species as its strains harbor and express either TcMAT1-1-1 or TcMAT1-2-1 genes as revealed by genome sequencing and RNAseq analyses. DNA-binding motifs located in their respective promoter regions appear to play a major role in the regulation of reproductive processes. The α-box and HMG-box domains are highly conserved along the Pezizomycetes and their strong structural similarity despite its poor sequence similarity supports a common evolutionary origin. Moreover, we set out a PCR-based approach to monitor the dynamics of T. claveryi strains of opposite mating type on mycorrhizal plants and soil. T. claveryi mycorrhizal plants at the nursery stage presented strains of both mating types, whereas a notable dominance of strains with the TcMAT1-1-1 gene was observed in field stage. Altogether, this research provides insights about genetic regulation and evolution of the MAT locus within the Pezizomycetes, and the reproductive biology of this important desert truffle, along with reliable markers to track the spatio-temporal distribution of strains of opposite mating types.","PeriodicalId":18965,"journal":{"name":"Mycorrhiza","volume":"36 3","pages":""},"PeriodicalIF":4.0,"publicationDate":"2026-05-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13152902/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147840327","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Ecotoxicological effect of imidacloprid on spore germination of phylogenetically distinct arbuscular mycorrhizal fungi species. 吡虫啉对不同系统发育的丛枝菌根真菌孢子萌发的生态毒理学影响。

IF 4 2区生物学

Mycorrhiza Pub Date : 2026-04-28 DOI: 10.1007/s00572-026-01263-6

Taynara Gomes Pires, Luís Carlos Iuñes de Oliveira Filho, Aline de Liz Ronsani, Osmar Klauberg-Filho

{"title":"Ecotoxicological effect of imidacloprid on spore germination of phylogenetically distinct arbuscular mycorrhizal fungi species.","authors":"Taynara Gomes Pires, Luís Carlos Iuñes de Oliveira Filho, Aline de Liz Ronsani, Osmar Klauberg-Filho","doi":"10.1007/s00572-026-01263-6","DOIUrl":"10.1007/s00572-026-01263-6","url":null,"abstract":"Arbuscular mycorrhizal fungi (AMF) establish a mutualistic symbiosis with most terrestrial plants. Mycorrhizal colonization begins with fungal spore germination in soil, a critical stage for symbiosis establishment, and is therefore highly susceptible. Among the compounds that may negatively affect AMF, imidacloprid has been identified as a potential stressor. In this context, the present study aimed to investigate the effects of the insecticide imidacloprid, as the active ingredient, on the pre-symbiotic phase of Acaulospora morrowiae, Gigaspora margarita, and Rhizophagus clarus. The substrate used for the in vitro assays was tropical artificial soil (TAS). The concentrations tested were 0, 2, 4, 8, 16, 32, 64, 128, 256, and 512 mg imidacloprid kg–1 of TAS for A. morrowiae and G. margarita; and 0, 1, 2, 4, 8, 16, 32, 64, 128, and 256 mg imidacloprid kg–1 of TAS for R. clarus. Imidacloprid reduced spore germination in all AMF species across all tested concentrations. However, A. morrowiae was the most sensitive species, with an IC50 of 23 mg kg–1. Although all AMF species evaluated in this study showed inhibition of spore germination upon exposure to imidacloprid, their responses differed among species, likely due to distinct morphological characteristics.","PeriodicalId":18965,"journal":{"name":"Mycorrhiza","volume":"36 3","pages":""},"PeriodicalIF":4.0,"publicationDate":"2026-04-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13124759/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147776689","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0