Molecular Brain最新文献_第6页

Database-assisted screening of autism spectrum disorder related gene set. 数据库辅助筛选自闭症谱系障碍相关基因组。

IF 3.3 3区医学

Molecular Brain Pub Date : 2024-08-09 DOI: 10.1186/s13041-024-01127-0

Éva Kereszturi

{"title":"Database-assisted screening of autism spectrum disorder related gene set.","authors":"Éva Kereszturi","doi":"10.1186/s13041-024-01127-0","DOIUrl":"10.1186/s13041-024-01127-0","url":null,"abstract":"Autism spectrum disorder (ASD) is a neurodevelopmental condition characterized by social and communication difficulties, along with repetitive behaviors. While genetic factors play a significant role in ASD, the precise genetic landscape remains complex and not fully understood, particularly in non-syndromic cases. The study performed an in silico comparison of three genetic databases. ClinVar, SFARI Gene, and AutDB were utilized to identify relevant gene subset and genetic variations associated with non-syndromic ASD. Gene set enrichment analysis (GSEA) and protein-protein interaction (PPI) network analysis were conducted to elucidate the biological significance of the identified genes. The integrity of ASD-related gene subset and the distribution of their variations were statistically assessed. A subset of twenty overlapping genes potentially specific for non-syndromic ASD was identified. GSEA revealed enrichment of biological processes related to neuronal development and differentiation, synaptic function, and social skills, highlighting their importance in ASD pathogenesis. PPI network analysis demonstrated functional relationships among the identified genes. Analysis of genetic variations showed predominance of rare variants and database-specific distribution patterns. The results provide valuable insights into the genetic landscape of ASD and outline the genes and biological processes involved in the condition, while taking into account that the study relied exclusively on in silico analyses, which may be subject to biases inherent to database methodologies. Further research incorporating multi-omics data and experimental validation is warranted to enhance our understanding of non-syndromic ASD genetics and facilitate the development of targeted research, interventions and therapies.","PeriodicalId":18851,"journal":{"name":"Molecular Brain","volume":"17 1","pages":"55"},"PeriodicalIF":3.3,"publicationDate":"2024-08-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11316361/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141913314","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Determinants of interactions of a novel next-generation gabapentinoid NVA1309 and mirogabalin with the Cavα2δ-1 subunit. 新型新一代加巴喷丁胺 NVA1309 和 mirogabalin 与 Cavα2δ-1 亚基相互作用的决定因素。

IF 3.3 3区医学

Molecular Brain Pub Date : 2024-08-07 DOI: 10.1186/s13041-024-01129-y

Ivana A Souza, Maria A Gandini, Md Yousof Ali, Franz Kricek, George Skouteris, Gerald W Zamponi

引用次数: 0

Astrocytic activation increases blood flow in the adult olfactory bulb. 星形胶质细胞的激活会增加成人嗅球的血流量。

IF 3.3 3区医学

Molecular Brain Pub Date : 2024-08-06 DOI: 10.1186/s13041-024-01126-1

Takashi Ogino, Masakazu Agetsuma, Masato Sawada, Hiroyuki Inada, Junichi Nabekura, Kazunobu Sawamoto

引用次数: 0

Research progress of brain organoids in the field of diabetes. 糖尿病领域的脑器官研究进展。

IF 3.3 3区医学

Molecular Brain Pub Date : 2024-08-06 DOI: 10.1186/s13041-024-01123-4

Ying Su, Aimei Liu, Hongguang Chen, Qingjie Chen, Bo Zhao, Runze Gao, Kangwei Zhang, Tie Peng, Zhenwang Zhang, Changhan Ouyang, Dan Zhu

引用次数: 0

Involvement of posterior hypothalamic CaMKII-positive neurons in ADHD-like behaviors in mice. 下丘脑后部 CaMKII 阳性神经元参与了小鼠的多动症样行为。

IF 3.3 3区医学

Molecular Brain Pub Date : 2024-08-05 DOI: 10.1186/s13041-024-01122-5

Changwoo Lee, Changsu Woo, Gyeong Ryeong Ma, Kyuhyun Choi, Shin Jung Kang, Ki Soon Shin

引用次数: 0

Intraplantar aminoglutethimide, a P450scc inhibitor, reduced the induction of mechanical allodynia in a rat model of thrombus-induced ischemic pain. 在血栓诱发缺血性疼痛的大鼠模型中，P450scc抑制剂氨鲁米特可减少机械异感的诱导。

IF 3.3 3区医学

Molecular Brain Pub Date : 2024-08-02 DOI: 10.1186/s13041-024-01125-2

Soon-Gu Kwon, Hoon-Seong Choi, Seo-Yeon Yoon, Dae-Hyun Roh, Jang-Hern Lee

{"title":"Intraplantar aminoglutethimide, a P450scc inhibitor, reduced the induction of mechanical allodynia in a rat model of thrombus-induced ischemic pain.","authors":"Soon-Gu Kwon, Hoon-Seong Choi, Seo-Yeon Yoon, Dae-Hyun Roh, Jang-Hern Lee","doi":"10.1186/s13041-024-01125-2","DOIUrl":"10.1186/s13041-024-01125-2","url":null,"abstract":"Neuroactive steroids (NASs) directly affect neuronal excitability. Despite their role in the nervous system is intimately linked to pain control, knowledge is currently limited. This study investigates the peripheral involvement of NASs in chronic ischemic pain by targeting the cytochrome P450 side-chain cleavage enzyme (P450scc). Using a rat model of hind limb thrombus-induced ischemic pain (TIIP), we observed an increase in P450scc expression in the ischemic hind paw skin. Inhibiting P450scc with intraplantar aminoglutethimide (AMG) administration from post-operative day 0 to 3 significantly reduced the development of mechanical allodynia. However, AMG administration from post-operative day 3 to 6 did not affect established mechanical allodynia. In addition, we explored the role of the peripheral sigma-1 receptor (Sig-1R) by co-administering PRE-084 (PRE), a Sig-1R agonist, with AMG. PRE reversed the analgesic effects of AMG during the induction phase. These findings indicate that inhibiting steroidogenesis with AMG alleviates peripheral ischemic pain during the induction phase via Sig-1Rs.","PeriodicalId":18851,"journal":{"name":"Molecular Brain","volume":"17 1","pages":"50"},"PeriodicalIF":3.3,"publicationDate":"2024-08-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11295590/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141879113","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Neurexin-3 in the paraventricular nucleus of the hypothalamus regulates body weight and glucose homeostasis independently of food intake. 下丘脑室旁核中的 Neurexin-3 与食物摄入无关，可调节体重和葡萄糖稳态。

IF 3.3 3区医学

Molecular Brain Pub Date : 2024-08-01 DOI: 10.1186/s13041-024-01124-3

Mingdao Mu, Haoyu Sun, Shuyan Geng, Tianxiang Xu, Chuanyao Sun, Zixu Zhang, Sibie Meng, Moyi Li, An Liu, Zhiyuan Yang, Wei Xie

{"title":"Neurexin-3 in the paraventricular nucleus of the hypothalamus regulates body weight and glucose homeostasis independently of food intake.","authors":"Mingdao Mu, Haoyu Sun, Shuyan Geng, Tianxiang Xu, Chuanyao Sun, Zixu Zhang, Sibie Meng, Moyi Li, An Liu, Zhiyuan Yang, Wei Xie","doi":"10.1186/s13041-024-01124-3","DOIUrl":"10.1186/s13041-024-01124-3","url":null,"abstract":"Neurexin-3 (Nrxn3) has been genetically associated with obesity, but the underlying neural mechanisms remain poorly understood. This study aimed to investigate the role of Nrxn3 in the paraventricular nucleus of the hypothalamus (PVN) in regulating energy balance and glucose homeostasis. We found that Nrxn3 expression in the PVN was upregulated in response to metabolic stressors, including cold exposure and fasting. Using Cre-loxP technology, we selectively ablated Nrxn3 in CaMKIIα-expressing neurons of the PVN in male mice. This genetic manipulation resulted in marked weight gain attributable to increased adiposity and impaired glucose tolerance, without affecting food intake. Our findings identify PVN CaMKIIα-expressing neurons as a critical locus where Nrxn3 modulates energy balance by regulating adipogenesis and glucose metabolism, independently of appetite. These results reveal a novel neural mechanism potentially linking Nrxn3 dysfunction to obesity pathogenesis, suggesting that targeting PVN Nrxn3-dependent neural pathways may inform new therapeutic approaches for obesity prevention and treatment.","PeriodicalId":18851,"journal":{"name":"Molecular Brain","volume":"17 1","pages":"49"},"PeriodicalIF":3.3,"publicationDate":"2024-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11295692/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141875361","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Systemic inflammation attenuates the repair of damaged brains through reduced phagocytic activity of monocytes infiltrating the brain. 全身性炎症会降低浸润大脑的单核细胞的吞噬活性，从而削弱受损大脑的修复能力。

IF 3.3 3区医学

Molecular Brain Pub Date : 2024-07-29 DOI: 10.1186/s13041-024-01116-3

Sushil Gaire, Jiawei An, Haijie Yang, Keon Ah Lee, Manisha Dumre, Eun Jeong Lee, Sang-Myun Park, Eun-Hye Joe

{"title":"Systemic inflammation attenuates the repair of damaged brains through reduced phagocytic activity of monocytes infiltrating the brain.","authors":"Sushil Gaire, Jiawei An, Haijie Yang, Keon Ah Lee, Manisha Dumre, Eun Jeong Lee, Sang-Myun Park, Eun-Hye Joe","doi":"10.1186/s13041-024-01116-3","DOIUrl":"10.1186/s13041-024-01116-3","url":null,"abstract":"In this study, we examined how systemic inflammation affects repair of brain injury. To this end, we created a brain-injury model by stereotaxic injection of ATP, a damage-associated molecular pattern component, into the striatum of mice. Systemic inflammation was induced by intraperitoneal injection of lipopolysaccharide (LPS-ip). An analysis of magnetic resonance images showed that LPS-ip reduced the initial brain injury but slowed injury repair. An immunostaining analysis using the neuronal marker, NeuN, showed that LPS-ip delayed removal of dead/dying neurons, despite the fact that LPS-ip enhanced infiltration of monocytes, which serve to phagocytize dead cells/debris. Notably, infiltrating monocytes showed a widely scattered distribution. Bulk RNAseq analyses showed that LPS-ip decreased expression of genes associated with phagocytosis, with PCR and immunostaining of injured brains confirming reduced levels of Cd68 and Clec7a, markers of phagocytic activity, in monocytes. Collectively, these results suggest that systemic inflammation affects properties of blood monocytes as well as brain cells, resulting in delay in clearing damaged cells and activating repair processes.","PeriodicalId":18851,"journal":{"name":"Molecular Brain","volume":"17 1","pages":"47"},"PeriodicalIF":3.3,"publicationDate":"2024-07-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11288066/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141792929","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

β-hydroxybutyrate and ischemic stroke: roles and mechanisms. β-羟丁酸与缺血性中风：作用与机制。

IF 3.3 3区医学

Molecular Brain Pub Date : 2024-07-29 DOI: 10.1186/s13041-024-01119-0

Ge Feng, Zongkai Wu, Leyi Yang, Kaimeng Wang, Hebo Wang

引用次数: 0

Spared nerve injury leads to reduced activity of neurons projecting from the ventrolateral periaqueductal gray to the locus coeruleus. 幸免神经损伤会导致从腹外侧下腹灰质投射到神经节的神经元活动减弱。

IF 3.3 3区医学

Molecular Brain Pub Date : 2024-07-24 DOI: 10.1186/s13041-024-01121-6

Wing Lam Yu, Zizhen Zhang, Gerald W Zamponi

{"title":"Spared nerve injury leads to reduced activity of neurons projecting from the ventrolateral periaqueductal gray to the locus coeruleus.","authors":"Wing Lam Yu, Zizhen Zhang, Gerald W Zamponi","doi":"10.1186/s13041-024-01121-6","DOIUrl":"10.1186/s13041-024-01121-6","url":null,"abstract":"The ventrolateral periaqueductal gray (vlPAG) serves as a central hub for descending pain modulation. It receives upstream projections from the medial prefrontal cortex (mPFC) and the ventrolateral orbitofrontal cortex (vlOFC), and projects downstream to the locus coeruleus (LC) and the rostroventral medulla (RVM). While much research has focused on upstream circuits and the LC-RVM connection, less is known about the PAG-LC circuit and its involvement in neuropathic pain. Here we examined the intrinsic electrophysiological properties of vlPAG-LC projecting neurons in Sham and spared nerve injury (SNI) operated mice. Injection of the retrotracer Cholera Toxin Subunit B (CTB-488) into the LC allowed the identification of LC-projecting neurons in the vlPAG. Electrophysiological recordings from CTB-488 positive cells revealed that both GABAergic and glutamatergic cells that project to the LC exhibited reduced intrinsic excitability after peripheral nerve injury. By contrast, CTB-488 negative cells did not exhibit alterations in firing properties after SNI surgery. An SNI-induced reduction of LC projecting cells was confirmed with c-fos labeling. Hence, SNI induces plasticity changes in the vlPAG that are consistent with a reduction in the descending modulation of pain signals.","PeriodicalId":18851,"journal":{"name":"Molecular Brain","volume":"17 1","pages":"46"},"PeriodicalIF":3.3,"publicationDate":"2024-07-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11267953/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141759836","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0