Molecular Brain最新文献

Circadian cilia transcriptome in mouse brain across physiological and pathological states. 小鼠大脑中跨越生理和病理状态的昼夜节律纤毛转录组

IF 3.3 3区医学

Molecular Brain Pub Date : 2024-09-20 DOI: 10.1186/s13041-024-01143-0

Kiki Chen, Kousha Changizi Ashtiani, Roudabeh Vakil Monfared, Pierre Baldi, Amal Alachkar

{"title":"Circadian cilia transcriptome in mouse brain across physiological and pathological states.","authors":"Kiki Chen, Kousha Changizi Ashtiani, Roudabeh Vakil Monfared, Pierre Baldi, Amal Alachkar","doi":"10.1186/s13041-024-01143-0","DOIUrl":"10.1186/s13041-024-01143-0","url":null,"abstract":"Primary cilia are dynamic sensory organelles that continuously undergo structural modifications in response to environmental and cellular signals, many of which exhibit rhythmic patterns. Building on our previous findings of rhythmic cilia-related gene expression in diurnal primates (baboon), this study extends the investigation to the nocturnal mouse brain to identify circadian patterns of cilia gene expression across brain regions. We used computational techniques and transcriptomic data from four publicly available databases, to examine the circadian expression of cilia-associated genes within six brain areas: brainstem, cerebellum, hippocampus, hypothalamus, striatum, and suprachiasmatic nucleus. Our analysis reveals that a substantial proportion of cilia transcripts exhibit circadian rhythmicity across the examined regions, with notable overrepresentation in the striatum, hippocampus, and cerebellum. We also demonstrate region-specific variations in the abundance and timing of circadian cilia genes' peaks, indicating an adaptation to the distinct physiological roles of each brain region. Additionally, we show that the rhythmic patterns of cilia transcripts are shifted under various physiological and pathological conditions, including modulation of the dopamine system, high-fat diet, and epileptic conditions, indicating the adaptable nature of cilia transcripts' oscillation. While limited to a few mouse brain regions, our study provides initial insights into the distinct circadian patterns of cilia transcripts and highlights the need for future research to expand the mapping across wider brain areas to fully understand the role of cilia's spatiotemporal dynamics in brain functions.","PeriodicalId":18851,"journal":{"name":"Molecular Brain","volume":null,"pages":null},"PeriodicalIF":3.3,"publicationDate":"2024-09-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11414107/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142291530","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

TRPM4 inhibition slows neuritogenesis progression of cortical neurons 抑制 TRPM4 可减缓大脑皮层神经元的神经发生进程

IF 3.6 3区医学

Molecular Brain Pub Date : 2024-09-12 DOI: 10.1186/s13041-024-01140-3

Denise Riquelme, Nicole Juanchuto-Viertel, Carlos Álamos, Elias Leiva-Salcedo

引用次数: 0

Simulated weightlessness procedure, head-down bed rest has reversible effects on the metabolism of rhesus macaque. 模拟失重过程、低头卧床休息对猕猴的新陈代谢有可逆的影响。

IF 3.3 3区医学

Molecular Brain Pub Date : 2024-09-03 DOI: 10.1186/s13041-024-01133-2

Yuting Li, Xu Zhang, Zhen Xu, Xixia Chu, Zhiqiang Hu, Zhengyang Ye, Caiqin Li, Zhenbo Wang, Bin Zeng, Jingyu Pan, Qian Zhao, Chengbin Zhou, Zhaohui Lan, Guanghan Kan, Guang He, Xiaodan Xu, Weidong Li

{"title":"Simulated weightlessness procedure, head-down bed rest has reversible effects on the metabolism of rhesus macaque.","authors":"Yuting Li, Xu Zhang, Zhen Xu, Xixia Chu, Zhiqiang Hu, Zhengyang Ye, Caiqin Li, Zhenbo Wang, Bin Zeng, Jingyu Pan, Qian Zhao, Chengbin Zhou, Zhaohui Lan, Guanghan Kan, Guang He, Xiaodan Xu, Weidong Li","doi":"10.1186/s13041-024-01133-2","DOIUrl":"10.1186/s13041-024-01133-2","url":null,"abstract":"It is a consensus in the international manned space field that factors such as microgravity during the space flight can cause anxiety, depression and other important brain function abnormalities in astronauts. However, the neural mechanism at the molecular level is still unclear. Due to the limitations of research conditions, studies of biological changes in the primate brain have been comparatively few. We took advantage of -6° head-down bed rest (HDBR), one of the most implemented space analogues on the ground, to investigate the effects of simulated weightlessness on non-human primate brain metabolites. The Rhesus Macaque monkeys in the experiment were divided into three groups: the control group, the 42-day simulated weightlessness group with HDBR, and the recovery group, which had 28 days of free activity in the home cage after the HDBR. Liquid chromatography-mass spectrometry (LC-MS) was used to perform metabolomics analysis on specific brain areas of the monkeys under three experimental conditions. Our results show that simulated weightlessness can cause neurotransmitter imbalances, the amino acid and energy metabolism disorders, and hormone disturbances. But these metabolomics changes are reversible after recovery. Our study suggests that long-term brain damage in space flight might be reversible at the metabolic level. This lays a technical foundation for ensuring brain health and enhancing the brain function in future space studies.","PeriodicalId":18851,"journal":{"name":"Molecular Brain","volume":null,"pages":null},"PeriodicalIF":3.3,"publicationDate":"2024-09-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11370317/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142126172","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Absence of ATG9A and synaptophysin demixing on Rab5 mutation-induced giant endosomes. Rab5突变诱导的巨型内体上缺乏ATG9A和突触素脱混。

IF 3.3 3区医学

Molecular Brain Pub Date : 2024-09-02 DOI: 10.1186/s13041-024-01132-3

Jiyoung Choi, Yumei Wu, Daehun Park

引用次数: 0

Electroacupuncture reduces inflammatory damage following cerebral ischemia-reperfusion by enhancing ABCA1-mediated efferocytosis in M2 microglia. 电针通过提高 M2 小胶质细胞 ABCA1 介导的排泄功能减轻脑缺血再灌注后的炎症损伤。

IF 3.3 3区医学

Molecular Brain Pub Date : 2024-09-02 DOI: 10.1186/s13041-024-01135-0

Yu-Sha Liao, Tie-Chun Zhang, Yu-Qi Tang, Pei Yu, Ya-Ning Liu, Jing Yuan, Ling Zhao

{"title":"Electroacupuncture reduces inflammatory damage following cerebral ischemia-reperfusion by enhancing ABCA1-mediated efferocytosis in M2 microglia.","authors":"Yu-Sha Liao, Tie-Chun Zhang, Yu-Qi Tang, Pei Yu, Ya-Ning Liu, Jing Yuan, Ling Zhao","doi":"10.1186/s13041-024-01135-0","DOIUrl":"10.1186/s13041-024-01135-0","url":null,"abstract":"Ischemic stroke (IS) is a severe cerebrovascular disease with high disability and mortality rates, where the inflammatory response is crucial to its progression and prognosis. Efferocytosis, the prompt removal of dead cells, can reduce excessive inflammation after IS injury. While electroacupuncture (EA) has been shown to decrease inflammation post-ischemia/reperfusion (I/R), its link to efferocytosis is unclear. Our research identified ATP-binding cassette transporter A1 (Abca1) as a key regulator of the engulfment process of efferocytosis after IS by analyzing public datasets and validating findings in a mouse model, revealing its close ties to IS progression. We demonstrated that EA can reduce neuronal cell death and excessive inflammation caused by I/R. Furthermore, EA treatment increased Abca1 expression, prevented microglia activation, promoted M2 microglia polarization, and enhanced their ability to phagocytose injured neurons in I/R mice. This suggests that EA's modulation of efferocytosis could be a potential mechanism for reducing cerebral I/R injury, making regulators of efferocytosis steps a promising therapeutic target for EA benefits.","PeriodicalId":18851,"journal":{"name":"Molecular Brain","volume":null,"pages":null},"PeriodicalIF":3.3,"publicationDate":"2024-09-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11367741/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142120239","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Felodipine attenuates neuroinflammatory responses and tau hyperphosphorylation through JNK/P38 signaling in tau-overexpressing AD mice. 非洛地平通过JNK/P38信号转导减轻tau过表达AD小鼠的神经炎症反应和tau高磷酸化。

IF 3.3 3区医学

Molecular Brain Pub Date : 2024-09-02 DOI: 10.1186/s13041-024-01137-y

Jeong-Woo Hwang, Jeongha Kim, Jin-Hee Park, Jinhan Nam, Ji-Yeong Jang, Aran Jo, Hyun-Ju Lee, Hyang-Sook Hoe

{"title":"Felodipine attenuates neuroinflammatory responses and tau hyperphosphorylation through JNK/P38 signaling in tau-overexpressing AD mice.","authors":"Jeong-Woo Hwang, Jeongha Kim, Jin-Hee Park, Jinhan Nam, Ji-Yeong Jang, Aran Jo, Hyun-Ju Lee, Hyang-Sook Hoe","doi":"10.1186/s13041-024-01137-y","DOIUrl":"10.1186/s13041-024-01137-y","url":null,"abstract":"We previously demonstrated that felodipine, an L-type calcium channel blocker, inhibits LPS-mediated neuroinflammatory responses in BV2 microglial cells and wild-type mice. However, the effects of felodipine on tau pathology, a hallmark of Alzheimer's disease (AD), have not been explored yet. Therefore, in the present study, we determined whether felodipine affects neuroinflammation and tau hyperphosphorylation in 3-month-old P301S transgenic mice (PS19), an early phase AD mice model for tauopathy. Felodipine administration decreased tauopathy-mediated microglial activation and NLRP3 expression in PS19 mice but had no effect on tauopathy-associated astrogliosis. In addition, felodipine treatment significantly reduced tau hyperphosphorylation at S202/Thr205 and Thr212/Ser214 residues via inhibiting JNK/P38 signaling in PS19 mice. Collectively, our results suggest that felodipine significantly ameliorates tau hyper-phosphorylation and tauopathy-associated neuroinflammatory responses in AD mice model for tauopathy and could be a novel therapeutic agent for AD.","PeriodicalId":18851,"journal":{"name":"Molecular Brain","volume":null,"pages":null},"PeriodicalIF":3.3,"publicationDate":"2024-09-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11367747/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142120240","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Running-induced neurogenesis reduces CA1 perineuronal net density without substantial temporal delay. 奔跑诱导的神经发生降低了CA1神经元周围的净密度，但没有明显的时间延迟。

IF 3.3 3区医学

Molecular Brain Pub Date : 2024-09-02 DOI: 10.1186/s13041-024-01138-x

Dylan J Terstege, Duneesha Goonetilleke, Cindy K Barha, Jonathan R Epp

{"title":"Running-induced neurogenesis reduces CA1 perineuronal net density without substantial temporal delay.","authors":"Dylan J Terstege, Duneesha Goonetilleke, Cindy K Barha, Jonathan R Epp","doi":"10.1186/s13041-024-01138-x","DOIUrl":"10.1186/s13041-024-01138-x","url":null,"abstract":"Aerobic exercise has many effects on brain function, particularly at the hippocampus. Exercise has been shown to increase the rate of adult neurogenesis within the dentate gyrus and decrease the density of perineuronal nets in area CA1. The relationship between the rate of neurogenesis and the density of perineuronal nets in CA1 is robust; however, these studies only ever examined these effects across longer time scales, with running manipulations of 4 weeks or longer. With such long periods of manipulation, the precise temporal nature of the relationship between running-induced neurogenesis and reduced perineuronal net density in CA1 is unknown. Here, we provided male and female mice with home cage access to running wheels for 0, 1, 2, or 4 weeks and quantified hippocampal neurogenesis and CA1 perineuronal net density. In doing so, we observed a 2-week delay period prior to the increase in neurogenesis, which coincided with the same delay prior to decreased CA1 perineuronal net density. These results highlight the closely linked temporal relationship between running-induced neurogenesis and decreased perineuronal net expression in CA1.","PeriodicalId":18851,"journal":{"name":"Molecular Brain","volume":null,"pages":null},"PeriodicalIF":3.3,"publicationDate":"2024-09-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11370122/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142120252","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Altered brain connectivity in mild cognitive impairment is linked to elevated tau and phosphorylated tau, but not to GAP-43 and Amyloid-β measurements: a resting-state fMRI study. 轻度认知障碍患者大脑连通性的改变与 tau 和磷酸化 tau 的升高有关，但与 GAP-43 和淀粉样蛋白-β 的测量无关：一项静息态 fMRI 研究。

IF 3.3 3区医学

Molecular Brain Pub Date : 2024-08-30 DOI: 10.1186/s13041-024-01136-z

Mohammad Sadeghi, Ali Azargoonjahromi, Hamide Nasiri, Arash Yaghoobi, Maryam Sadeghi, Seyedeh Saeideh Chavoshi, Shilan Baghaeikia, Nastaran Mahzari, Arina Valipour, Romina Razeghi Oskouei, Farshad Shahkarami, Fatemeh Amiri, Mahsa Mayeli

{"title":"Altered brain connectivity in mild cognitive impairment is linked to elevated tau and phosphorylated tau, but not to GAP-43 and Amyloid-β measurements: a resting-state fMRI study.","authors":"Mohammad Sadeghi, Ali Azargoonjahromi, Hamide Nasiri, Arash Yaghoobi, Maryam Sadeghi, Seyedeh Saeideh Chavoshi, Shilan Baghaeikia, Nastaran Mahzari, Arina Valipour, Romina Razeghi Oskouei, Farshad Shahkarami, Fatemeh Amiri, Mahsa Mayeli","doi":"10.1186/s13041-024-01136-z","DOIUrl":"10.1186/s13041-024-01136-z","url":null,"abstract":"Mild Cognitive Impairment (MCI) is a neurological condition characterized by a noticeable decline in cognitive abilities that falls between normal aging and dementia. Along with some biomarkers like GAP-43, Aβ, tau, and P-tau, brain activity and connectivity are ascribed to MCI; however, the link between brain connectivity changes and such biomarkers in MCI is still being investigated. This study explores the relationship between biomarkers like GAP-43, Aβ, tau, and P-tau, and brain connectivity. We enrolled 25 Participants with normal cognitive function and 23 patients with MCI. Levels of GAP-43, Aβ1-42, t-tau, and p-tau181p in the CSF were measured, and functional connectivity measures including ROI-to-voxel (RV) correlations and the DMN RV-ratio were extracted from the resting-state fMRI data. P-values below 0.05 were considered significant. The results showed that in CN individuals, higher connectivity within the both anterior default mode network (aDMN) and posterior DMN (pDMN) was associated with higher levels of the biomarker GAP-43. In contrast, MCI individuals showed significant negative correlations between DMN connectivity and levels of tau and P-tau. Notably, no significant correlations were found between Aβ levels and connectivity measures in either group. These findings suggest that elevated levels of GAP-43 indicate increased functional connectivity in aDMN and pDMN. Conversely, elevated levels of tau and p-tau can disrupt connectivity through various mechanisms. Thus, the accumulation of tau and p-tau can lead to impaired neuronal connectivity, contributing to cognitive decline.","PeriodicalId":18851,"journal":{"name":"Molecular Brain","volume":null,"pages":null},"PeriodicalIF":3.3,"publicationDate":"2024-08-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11363600/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142109621","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Astrocyte-derived dominance winning reverses chronic stress-induced depressive behaviors. 源自星形胶质细胞的优势胜势可逆转慢性压力诱发的抑郁行为

IF 3.3 3区医学

Molecular Brain Pub Date : 2024-08-27 DOI: 10.1186/s13041-024-01134-1

Kyungchul Noh, Junyoung Oh, Woo-Hyun Cho, Minkyu Hwang, Sung Joong Lee

{"title":"Astrocyte-derived dominance winning reverses chronic stress-induced depressive behaviors.","authors":"Kyungchul Noh, Junyoung Oh, Woo-Hyun Cho, Minkyu Hwang, Sung Joong Lee","doi":"10.1186/s13041-024-01134-1","DOIUrl":"10.1186/s13041-024-01134-1","url":null,"abstract":"Individuals with low social status are at heightened risk of major depressive disorder (MDD), and MDD also influences social status. While the interrelationship between MDD and social status is well-defined, the behavioral causality between these two phenotypes remains unexplored. Here, we investigated the behavioral relationships between depressive and dominance behaviors in male mice exposed to chronic restraint stress and the role of medial prefrontal cortex (mPFC) astrocytes in these behaviors. Chronic restraint stress induced both depressive and submissive behaviors. Chemogenetic mPFC astrocyte activation significantly enhanced dominance in chronic stress-induced submissive mice by increasing the persistence of defensive behavior, although it did not affect depressive behaviors. Notably, repetitive winning experiences following mPFC astrocyte stimulation exerted anti-depressive effects in chronic restraint stress-induced depressive mice. These data indicate that mPFC astrocyte-derived winning experience renders anti-depressive effects, and may offer a new strategy for treating depression caused by low status in social hierarchies by targeting mPFC astrocytes.","PeriodicalId":18851,"journal":{"name":"Molecular Brain","volume":null,"pages":null},"PeriodicalIF":3.3,"publicationDate":"2024-08-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11351830/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142080961","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Protocadherin 19 regulates axon guidance in the developing Xenopus retinotectal pathway. 原粘连蛋白19调节发育中的章鱼视网膜通路的轴突导向。

IF 3.3 3区医学

Molecular Brain Pub Date : 2024-08-22 DOI: 10.1186/s13041-024-01130-5

Jane Jung, Jugeon Park, Sihyeon Park, Chul Hoon Kim, Hosung Jung

引用次数: 0