MycologyPub Date : 2023-01-01DOI: 10.1080/21501203.2022.2103194
Li-Wei Zhou, Tom W May
{"title":"Fungal taxonomy: current status and research agendas for the interdisciplinary and globalisation era.","authors":"Li-Wei Zhou, Tom W May","doi":"10.1080/21501203.2022.2103194","DOIUrl":"https://doi.org/10.1080/21501203.2022.2103194","url":null,"abstract":"<p><p>Fungal taxonomy is a fundamental discipline that aims to recognise all fungi and their kinships. Approximately 5% of a practical estimate of 2.2-3.8 million species globally are currently known, and consequently the Fungal Tree of Life (FTOL) is very incompletely reconstructed. With the advances of new technologies, mycology is marching into the interdisciplinary and globalisation era. To make fungal taxonomy relevant, innovative sampling methods and phylogenomics analyses should be performed to reconstruct a much more comprehensive FTOL. In association with this densely sampled FTOL, multiomics will reveal what drives fungal species diversification and how fungal traits evolve to adapt to various environments, while metagenomics will facilitate the understanding and protection of the ecological functions of fungi. A coordinated approach to pursuing these research agendas that includes conceiving of and costing a mission to describe all the fungi on the planet will unlock potential of fungi to support sustainable development of our society.</p>","PeriodicalId":18833,"journal":{"name":"Mycology","volume":"14 1","pages":"52-59"},"PeriodicalIF":4.2,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9930751/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9314923","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
MycologyPub Date : 2023-01-01DOI: 10.1080/21501203.2022.2143919
Jingzu Sun, Shuang Yu, Yongzhong Lu, Hongwei Liu, Xingzhong Liu
{"title":"Proposal of a new family <i>Pseudodiploösporeaceae</i> fam. nov. (<i>Hypocreales</i>) based on phylogeny of <i>Diploöspora longispora</i> and <i>Paecilomyces penicillatus</i>.","authors":"Jingzu Sun, Shuang Yu, Yongzhong Lu, Hongwei Liu, Xingzhong Liu","doi":"10.1080/21501203.2022.2143919","DOIUrl":"https://doi.org/10.1080/21501203.2022.2143919","url":null,"abstract":"<p><p>During a field survey of cultivated <i>Morchella</i> mushroom diseases, <i>Diploöspora longispora</i> and <i>Paecilomyces penicillatus</i>, causal agents of pileus rot or white mould disease were detected, which resulted in up to 80% of yield losses. Multi-locus phylogenic analysis revealed that the fungi were affiliated in a distinct clade in <i>Hypocreales</i>. We further constructed a phylogenetic tree with broader sampling in <i>Hypocreales</i> and estimated the divergence times. The <i>D. longispora</i> and <i>P. penicillatus</i> clades were estimated to have diverged from <i>Hypocreaceae</i> around 129 MYA and <i>Pseudodiploösporeaceae</i> fam. nov is herein proposed to accommodate species in this clade. Two new genera, i.e. <i>Pseudodiploöspora</i> and <i>Zelopaecilomyceswere,</i> were introduced based on morphological characteristics and phylogenic relationships of <i>Diploöspora</i> longispora and <i>Paecilomyces penicillatus</i>, respectively. Five new combinations - <i>Pseudodiploöspora cubensis, P. longispora, P. fungicola, P. zinniae</i>, and <i>Zelopaecilomyces penicillatus</i> - were proposed.</p>","PeriodicalId":18833,"journal":{"name":"Mycology","volume":"14 1","pages":"60-73"},"PeriodicalIF":4.2,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9930807/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9314924","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
MycologyPub Date : 2023-01-01DOI: 10.1080/21501203.2022.2089262
Peng Zhao, Yan Li, Yuanjie Li, Fang Liu, Junmin Liang, Xin Zhou, Lei Cai
{"title":"Applying early divergent characters in higher rank taxonomy of <i>Melampsorineae</i> (<i>Basidiomycota, Pucciniales</i>).","authors":"Peng Zhao, Yan Li, Yuanjie Li, Fang Liu, Junmin Liang, Xin Zhou, Lei Cai","doi":"10.1080/21501203.2022.2089262","DOIUrl":"https://doi.org/10.1080/21501203.2022.2089262","url":null,"abstract":"<p><p>Rust fungi in the order <i>Pucciniales</i> represent one of the largest groups of phytopathogens, which occur on mosses, ferns to advanced monocots and dicots. Seven suborders and 18 families have been reported so far, however recent phylogenetic studies have revealed para- or polyphyly of several morphologically defined suborders and families, particularly in <i>Melampsorineae</i>. In this study, a comprehensive phylogenetic framework was constructed based on a molecular phylogeny inferred from rDNA sequences of 160 species belonging to 16 genera in <i>Melampsorineae</i> (i.e. <i>Chrysomyxa, Cerospora, Coleopuccinia, Coleosporium, Cronartium, Hylospora, Melampsora, Melampsorella, Melampsoridium, Milesina, Naohidemyces, Pucciniastrum, Quasipucciniastrum, Rossmanomyces, Thekopsora, Uredinopsis</i>). Our phylogenetic inference indicated that 13 genera are monophyletic with strong supports, while <i>Pucciniastrum</i> is apparently polyphyletic. A new genus, <i>Nothopucciniastrum</i> was therefore established and segregated from <i>Pucciniastrum</i>, with ten new combinations proposed. At the family level, this study further demonstrates the importance of applying morphologies of spore-producing structures (basidia, spermogonia, aecia, uredinia and telia) in higher rank taxonomy, while those traditionally applied spore morphologies (basidiospores, spermatia, aeciospores, urediniospores and teliospores) represent later diverged characters that are more suitable for the taxonomy at generic and species levels. Three new families, <i>Hyalopsoraceae, Nothopucciniastraceae</i> and <i>Thekopsoraceae</i> were proposed based on phylogenetic and morphological distinctions, towards a further revision of <i>Pucciniales</i> in line with the phylogenetic relationships.</p>","PeriodicalId":18833,"journal":{"name":"Mycology","volume":"14 1","pages":"11-36"},"PeriodicalIF":4.2,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9930778/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9329379","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
MycologyPub Date : 2023-01-01DOI: 10.1080/21501203.2023.2237047
Jiarui Yang, Juanli Yun, Xingzhong Liu, Wenbin Du, Meichun Xiang
{"title":"Niche and ecosystem preference of earliest diverging fungi in soils.","authors":"Jiarui Yang, Juanli Yun, Xingzhong Liu, Wenbin Du, Meichun Xiang","doi":"10.1080/21501203.2023.2237047","DOIUrl":"https://doi.org/10.1080/21501203.2023.2237047","url":null,"abstract":"<p><p>Within the supergroup Rotosphaeromycetes, or \"Holomycota\"/\"Nucletmycea\", there are several well-recognised unicellular clades in the earliest diverging fungi (EDF). However, we know little about their occurrence. Here, we investigated EDF in the rhizosphere and bulk soils from cropland, forest, orchard, and wetland ecosystems around the Beijing-Hebei area, China, to illustrate their niche and ecosystem preference. More than 500 new operational taxonomic units (OTUs) of EDF were detected based on the 18S rRNA genes. Microsporida and Aphelida constitute dominant groups, whereas Rozellosporida was quite rare. Although the EDF community was site-specific, the soil chemical characteristics, vegetation, and other eukaryotic microorganisms were the key factors driving the occurrence of EDF. Moreover, the stochastic process consisted the most of the EDF community assembly.</p>","PeriodicalId":18833,"journal":{"name":"Mycology","volume":"14 3","pages":"239-255"},"PeriodicalIF":4.2,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/ff/5e/TMYC_14_2237047.PMC10424602.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10565652","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
MycologyPub Date : 2023-01-01DOI: 10.1080/21501203.2023.2213704
Mayasar I Al-Zaban, Ahlam H Alrokban, Mohamed A Mahmoud
{"title":"Development of a real-time PCR and multiplex PCR assay for the detection and identification of mycotoxigenic fungi in stored maize grains.","authors":"Mayasar I Al-Zaban, Ahlam H Alrokban, Mohamed A Mahmoud","doi":"10.1080/21501203.2023.2213704","DOIUrl":"https://doi.org/10.1080/21501203.2023.2213704","url":null,"abstract":"<p><p>This study aimed to identify important mycotoxigenic fungi and accurate detection of mycotoxin in stored maize grains using molecular methods. The current study also optimised the real-time PCR (RT-PCR) assay. The melting curve was established to identify isolated fungal species of <i>Aspergillus</i> (4), <i>Fusarium</i> (3), <i>Penicillium</i> (3), and <i>Alternaria</i> (one). A multiplex polymerase chain reaction (mPCR) technique was developed for the detection and characterisation of mycotoxin producing fungi, mycotoxin metabolic pathway genes, and the determination of eleven mycotoxins in stored maize grains using high-performance liquid chromatography (HPLC). The mPCR results indicated positive signals for potentially mycotoxigenic fungal species tested of <i>Aspergillus, Fusarium, Penicillium</i>, and <i>Alternaria</i>. A protocol for multiplex reverse transcription-polymerase chain reaction (mRT-PCR) was tested to distinguish between free and contaminated, stored maize with aflatoxin B1 (AFB1). The expression pattern of four aflatoxin biosynthetic pathway genes, AFB1 (<i>aflQ, aflP, aflO</i>, and <i>aflD</i>), was a good marker for contaminated, stored maize grains. HPLC analysis showed that maize grain samples were contaminated with mycotoxins, and the concentration was above the detection level. The results indicate that the polyphasic approach might provide a sensitive, rapid, and accurate method for detecting and identifying mycotoxigenic fungal species and mycotoxins in stored maize grains.</p>","PeriodicalId":18833,"journal":{"name":"Mycology","volume":"14 3","pages":"227-238"},"PeriodicalIF":4.2,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/db/93/TMYC_14_2213704.PMC10424615.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10250972","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
MycologyPub Date : 2023-01-01DOI: 10.1080/21501203.2023.2191636
B Van der Merwe, P Herrmann, Karin Jacobs
{"title":"Hericium ophelieae sp. nov., a novel species of Hericium (Basidiomycota: Russulales, Hericiaceae) from the Southern Afrotemperate forests of South Africa.","authors":"B Van der Merwe, P Herrmann, Karin Jacobs","doi":"10.1080/21501203.2023.2191636","DOIUrl":"https://doi.org/10.1080/21501203.2023.2191636","url":null,"abstract":"<p><p>A novel species of <i>Hericium</i> was recently collected in the Afrotemperate forests (Knysna - Amatole region) of Southern Africa. The novel species shares many similar, dentate features common to other species in <i>Hericium</i>, and its basidiome first appears stark white and yellows with age. However, the substrate choice and gloeocystidia and basidiospore sizes of the specimens collected were distinct from other <i>Hericium</i> species. This was confirmed by sequencing the ITS and 28S genetic markers, respectively. The novel species is described as <i>Hericium ophelieae</i> sp. nov. and appears unique as it grows on hardwoods indigenous to Southern Africa. The species has larger basidiospores and wider gloeocystidia compared to its closest relative. <i>H. ophelieae</i> sp. nov. is the first endemic species of the medicinal mushroom genus <i>Hericium</i> to be described from Southern Africa, and the second to be described from Africa, after its closest relative, <i>H. bembedjaense</i>, which was isolated in Cameroon. Although this is the first <i>Hericium</i> to be described from the Southern African region, there are likely others to be discovered, and this study highlights the need for further research into the fungal diversity of Afrotemperate environments.</p>","PeriodicalId":18833,"journal":{"name":"Mycology","volume":"14 2","pages":"133-141"},"PeriodicalIF":4.2,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10161958/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9784065","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Species diversity of pathogenic wood-rotting fungi (Agaricomycetes, Basidiomycota) in China.","authors":"Yuan Yuan, Lu-Sen Bian, Ying-Da Wu, Jia-Jia Chen, Fang Wu, Hong-Gao Liu, Guang-Yu Zeng, Yu-Cheng Dai","doi":"10.1080/21501203.2023.2238779","DOIUrl":"https://doi.org/10.1080/21501203.2023.2238779","url":null,"abstract":"<p><p>Wood-rotting basidiomycetes have been investigated in the Chinese forest ecosystem for the past 30 years. Two hundred and five pathogenic wood-decayers belonging to 9 orders, 30 families, and 74 genera have been found in Chinese native forests, plantations, and gardens. Seventy-two species (accounting for 35% of the total pathogenic species) are reported as pathogenic fungi in China for the first time. Among these pathogens, 184 species are polypores, nine are corticioid fungi, eight are agarics and five are hydnoid basidiomycetes. One hundred and seventy-seven species (accounting for 86%) cause white rot, while 28 species (accounting for 14%) result in brown rot; 157 species grow on angiosperm trees (accounting for 76.5%) and 44 species occur on gymnosperm trees (accounting for 21.5%), only four species inhabit both angiosperms and gymnosperms (accounting for 2%); 95 species are distributed in boreal to temperate forests and 110 in subtropical to tropical forests. In addition, 17 species, including <i>Fomitopsis pinicola</i>, <i>Heterobasidion parviporum</i>, and <i>Phellinidium weirii</i> etc. which were previously treated as pathogenic species in China, do not occur in China according to recent studies. In this paper, the host(s), type of forest, rot type, and distribution of each pathogenic species in China are given.</p>","PeriodicalId":18833,"journal":{"name":"Mycology","volume":"14 3","pages":"204-226"},"PeriodicalIF":4.2,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10424591/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10250971","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Four new species of <i>Phyllosticta</i> from China based on morphological and phylogenetic characterization.","authors":"Xiao-Nan Sui, Mei-Jun Guo, Hao Zhou, Cheng-Lin Hou","doi":"10.1080/21501203.2023.2225552","DOIUrl":"https://doi.org/10.1080/21501203.2023.2225552","url":null,"abstract":"<p><p><i>Phyllosticta</i> (<i>Phyllostictaceae, Botryosphaeriales</i>) species are widely distributed globally and constitute a diverse group of pathogenic and endophytic fungi associated with a broad range of plant hosts. In this study, four new species of <i>Phyllosticta</i>, i.e. <i>P. endophytica, P. jiangxiensis, P. machili</i>, and <i>P. xinyuensis</i>, were described using morphological characteristics and multi-locus phylogeny based on the internal transcribed spacer region (ITS) with intervening 5.8S rRNA gene, large subunit of rRNA gene (nrLSU), translation elongation factor 1-alpha gene (<i>tef1</i>), actin gene (<i>act</i>), and glyceraldehyde-3-phosphate dehydrogenase gene (<i>gapdh</i>). <i>Phyllosticta machili</i> is the first species of this genus reported to infect plants of the <i>Machilus</i> genus.</p>","PeriodicalId":18833,"journal":{"name":"Mycology","volume":"14 3","pages":"190-203"},"PeriodicalIF":4.2,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10424619/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10547145","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
MycologyPub Date : 2023-01-01DOI: 10.1080/21501203.2022.2096144
Meng Pan, Lu Lin, Chengming Tian, Xinlei Fan
{"title":"Identification and pathogenicity of six fungal species causing canker and dieback disease on golden rain tree in Beijing, China.","authors":"Meng Pan, Lu Lin, Chengming Tian, Xinlei Fan","doi":"10.1080/21501203.2022.2096144","DOIUrl":"https://doi.org/10.1080/21501203.2022.2096144","url":null,"abstract":"<p><p>Golden rain trees (<i>Koelreuteria paniculata</i>) are largely cultivated because of their important ornamental, medicinal, and economic value. However, they are affected by canker and dieback disease to a large extent. To determine the fungi associated with canker and dieback disease of golden rain trees, isolations were obtained from diseased branches and twigs during 2019 and 2020 in greenbelts and nurseries in Beijing, China. Isolates were identified as six species (<i>Allocryptovalsa castaneicola, Botryosphaeria dothidea, Cytospora koelreutericola</i> sp. nov., <i>Dothiorella acericola, Eutypella citricola</i>, and <i>Peroneutypa scoparia</i>) based on morphological features and phylogenetic analyses of ITS, <i>act, rpb2, tef1-α</i>, and <i>tub2</i>. The results of pathogenicity tests indicated that all fungi produced discoloration and <i>Botryosphaeria dothidea</i> was highly aggressive to golden rain tree. In conclusion, this study explored the taxonomy, phylogeny, and pathogenicity of different fungal species associated with canker and dieback disease on golden rain tree and provided fundamental knowledge to improve disease management.</p>","PeriodicalId":18833,"journal":{"name":"Mycology","volume":"14 1","pages":"37-51"},"PeriodicalIF":4.2,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9930857/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9329374","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Metabolomics analysis of mycelial exudates provides insights into fungal antagonists of <i>Armillaria</i>.","authors":"Jian Zhan, Jing Yuan, Jianwei Liu, Fengming Zhang, Fuqiang Yu, Yanliang Wang","doi":"10.1080/21501203.2023.2238753","DOIUrl":"https://doi.org/10.1080/21501203.2023.2238753","url":null,"abstract":"<p><p>The genus <i>Armillaria</i> has high edible and medical values, with zones of antagonism often occurring when different species are paired in culture on agar media, while the antagonism-induced metabolic alteration remains unclear. Here, the metabolome of mycelial exudates of two Chinese <i>Armillaria</i> biological species, C and G, co-cultured or cultured separately was analysed to discover the candidate biomarkers and the key metabolic pathways involved in <i>Armillaria</i> antagonists. A total of 2,377 metabolites were identified, mainly organic acids and derivatives, lipids and lipid-like molecules, and organoheterocyclic compounds. There were 248 and 142 differentially expressed metabolites between group C-G and C, C-G, and G, respectively, and fourteen common differentially expressed metabolites including malate, uracil, Leu-Gln-Arg, etc. Metabolic pathways like TCA cycle and pyrimidine metabolism were significantly affected by C-G co-culture. Additionally, 156 new metabolites (largely organic acids and derivatives) including 32 potential antifungal compounds, primarily enriched into biosynthesis of secondary metabolites pathways were identified in C-G co-culture mode. We concluded that malate and uracil could be used as the candidate biomarkers, and TCA cycle and pyrimidine metabolism were the key metabolic pathways involved in <i>Armillaria</i> antagonists. The metabolic changes revealed in this study provide insights into the mechanisms underlying fungal antagonists.</p>","PeriodicalId":18833,"journal":{"name":"Mycology","volume":"14 3","pages":"264-274"},"PeriodicalIF":4.2,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/02/ff/TMYC_14_2238753.PMC10424624.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10547148","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}