Xin Ouyang, Senhua Chen, Qiling Chen, Heng Guo, Lan Liu, Hongju Liu, Chong Yan
{"title":"抗炎多酮类化合物来源于内生真菌Hypomontagnella sp. TX-09。","authors":"Xin Ouyang, Senhua Chen, Qiling Chen, Heng Guo, Lan Liu, Hongju Liu, Chong Yan","doi":"10.1080/21501203.2024.2397600","DOIUrl":null,"url":null,"abstract":"<p><p>Four new lactones, including hypomonacid A (<b>1</b>) and hypomonone A-C (<b>4-6</b>), as well as nine known polyketide analogues (<b>2-3</b> and <b>7-13</b>) were obtained from endophytic fungus <i>Hypomontagnella</i> sp. TX-09 derived from <i>Santalum album</i>. Their planar structures were extensively established by analysing HRESIMS and NMR spectroscopic data. Stereochemistry of new compounds was determined by X-ray diffraction analysis and modified Mosher's method in combination with quantum-chemical ECD calculation. In addition, compounds <b>1</b> and <b>2</b> showed anti-inflammatory activity by inhibition of lipopolysaccharide (LPS)-induced NO production in RAW264.7 cells at 50 μmol/L without cytotoxicity. Among them, compound <b>1</b> inhibited the production of LPS-stimulated inflammation in mouse macrophage RAW264.7 cells by suppressing the expression of iNOS, TNF-α, IL-1β, and IL-6.</p>","PeriodicalId":18833,"journal":{"name":"Mycology","volume":"16 2","pages":"918-928"},"PeriodicalIF":4.4000,"publicationDate":"2024-10-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12096673/pdf/","citationCount":"0","resultStr":"{\"title\":\"Anti-inflammatory polyketides from <i>Santalum album</i> derived endophytic fungus <i>Hypomontagnella</i> sp. TX-09.\",\"authors\":\"Xin Ouyang, Senhua Chen, Qiling Chen, Heng Guo, Lan Liu, Hongju Liu, Chong Yan\",\"doi\":\"10.1080/21501203.2024.2397600\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>Four new lactones, including hypomonacid A (<b>1</b>) and hypomonone A-C (<b>4-6</b>), as well as nine known polyketide analogues (<b>2-3</b> and <b>7-13</b>) were obtained from endophytic fungus <i>Hypomontagnella</i> sp. TX-09 derived from <i>Santalum album</i>. Their planar structures were extensively established by analysing HRESIMS and NMR spectroscopic data. Stereochemistry of new compounds was determined by X-ray diffraction analysis and modified Mosher's method in combination with quantum-chemical ECD calculation. In addition, compounds <b>1</b> and <b>2</b> showed anti-inflammatory activity by inhibition of lipopolysaccharide (LPS)-induced NO production in RAW264.7 cells at 50 μmol/L without cytotoxicity. Among them, compound <b>1</b> inhibited the production of LPS-stimulated inflammation in mouse macrophage RAW264.7 cells by suppressing the expression of iNOS, TNF-α, IL-1β, and IL-6.</p>\",\"PeriodicalId\":18833,\"journal\":{\"name\":\"Mycology\",\"volume\":\"16 2\",\"pages\":\"918-928\"},\"PeriodicalIF\":4.4000,\"publicationDate\":\"2024-10-16\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12096673/pdf/\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Mycology\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.1080/21501203.2024.2397600\",\"RegionNum\":2,\"RegionCategory\":\"生物学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"2025/1/1 0:00:00\",\"PubModel\":\"eCollection\",\"JCR\":\"Q1\",\"JCRName\":\"MYCOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Mycology","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.1080/21501203.2024.2397600","RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2025/1/1 0:00:00","PubModel":"eCollection","JCR":"Q1","JCRName":"MYCOLOGY","Score":null,"Total":0}
引用次数: 0
摘要
从Santalum album中的内生真菌Hypomontagnella sp. x -09中获得了4个新的内酯，包括hypomononone A(1)和hypoomonone A- c(4-6)，以及9个已知的聚酮类似物（2-3和7-13）。通过分析hremam和NMR光谱数据，广泛地确定了它们的平面结构。通过x射线衍射分析和改进的Mosher法结合量子化学ECD计算确定了新化合物的立体化学性质。此外，化合物1和2在50 μmol/L浓度下，通过抑制脂多糖（LPS）诱导的RAW264.7细胞NO生成而显示抗炎活性，且无细胞毒性。其中，化合物1通过抑制iNOS、TNF-α、IL-1β和IL-6的表达，抑制lps刺激小鼠巨噬细胞RAW264.7细胞炎症的产生。
Anti-inflammatory polyketides from Santalum album derived endophytic fungus Hypomontagnella sp. TX-09.
Four new lactones, including hypomonacid A (1) and hypomonone A-C (4-6), as well as nine known polyketide analogues (2-3 and 7-13) were obtained from endophytic fungus Hypomontagnella sp. TX-09 derived from Santalum album. Their planar structures were extensively established by analysing HRESIMS and NMR spectroscopic data. Stereochemistry of new compounds was determined by X-ray diffraction analysis and modified Mosher's method in combination with quantum-chemical ECD calculation. In addition, compounds 1 and 2 showed anti-inflammatory activity by inhibition of lipopolysaccharide (LPS)-induced NO production in RAW264.7 cells at 50 μmol/L without cytotoxicity. Among them, compound 1 inhibited the production of LPS-stimulated inflammation in mouse macrophage RAW264.7 cells by suppressing the expression of iNOS, TNF-α, IL-1β, and IL-6.
求助内容：
应助结果提醒方式：
京公网安备 11010802042870号
