Mycology最新文献_第5页

Antimicrobial properties and biotransforming ability of fungal endophytes from Ficus carica L. (Moraceae). 桑科无花果内生真菌的抗菌特性和生物转化能力。

IF 4.2 2区生物学

Mycology Pub Date : 2023-01-01 DOI: 10.1080/21501203.2023.2175500

Melisa Isabel Barolo, María Victoria Castelli, Silvia Noelí López

{"title":"Antimicrobial properties and biotransforming ability of fungal endophytes from Ficus carica L. (Moraceae).","authors":"Melisa Isabel Barolo, María Victoria Castelli, Silvia Noelí López","doi":"10.1080/21501203.2023.2175500","DOIUrl":"https://doi.org/10.1080/21501203.2023.2175500","url":null,"abstract":"The endophytic fungal community associated with leaves of Ficus carica L. (Moraceae) from Argentina was investigated. Fifteen fungal isolates were isolated and identified by molecular methods into the genera Alternaria, Cladosporium, Curvularia, Diaporthe, Epicoccum, Myrothecium, Neofusicoccum, Nigrospora, Preussia and Ustilago. Cladosporium cladosporioides and Curvularia lunata were the most frequently isolated species. The fungal metabolic profiles were obtained by automated TLC and NMR and analysed by PC Analysis. Antifungal and antibacterial activity was assessed by bioautographic assays. In addition, the biotransforming ability of the fungal isolates was tested on F. carica extracts. Five isolates (33.3%) exhibited inhibitory activity against at least one of the microorganisms tested. Most of the fungal endophytes were able to metabolise the flavonoid rutin 1, and the coumarin psoralen 3 present in F. carica extracts. Further investigations of the psoralen biotransforming ability performed by the selected endophyte Alternaria alternata F8 showed the accumulation of the 6,7-furan-hydrocoumaric acid derivative 4 as the main biotransformation product. Our results corroborate that F. carica can live symbiotically with rich and diverse endophytic communities adding insights about their ecological interactions.","PeriodicalId":18833,"journal":{"name":"Mycology","volume":"14 2","pages":"108-132"},"PeriodicalIF":4.2,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/c6/5a/TMYC_14_2175500.PMC10161954.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9784068","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Tools and basic procedures of gene manipulation in nematode-trapping fungi. 捕获线虫真菌基因操作的工具和基本程序。

IF 4.2 2区生物学

Mycology Pub Date : 2023-01-01 DOI: 10.1080/21501203.2023.2165186

Shunxian Wang, Xingzhong Liu

{"title":"Tools and basic procedures of gene manipulation in nematode-trapping fungi.","authors":"Shunxian Wang, Xingzhong Liu","doi":"10.1080/21501203.2023.2165186","DOIUrl":"https://doi.org/10.1080/21501203.2023.2165186","url":null,"abstract":"Nematode-trapping fungi (NTF) are the majority of carnivorous microbes to capture nematodes through diverse and sophisticated trapping organs derived from hyphae. They can adopt carnivorous lifestyles in addition to saprophytism to obtain extra-nutrition from nematodes. As a special group of fungi, the NTF are not only excellent model organism for studying lifestyle transition of fungi but also natural resources of exploring biological control of nematodes. However, the carnivorous mechanism of NTF remains poorly understood. Nowadays, the omics studies of NTF have provided numerous genes and pathways that are associated with the phenotypes of carnivorous traits, which need molecular tools to verify. Here, we review the development and progress of gene manipulation tools in NTF, including methodology and strategy of transformation, random gene mutagenesis methods and target gene mutagenesis methods. The principle and practical approach for each method was summarized and discussed, and the basic operational flow for each tool was described. This paper offers a clear reference and instruction for researchers who work on NTF as well as other group of fungi.","PeriodicalId":18833,"journal":{"name":"Mycology","volume":"14 2","pages":"75-90"},"PeriodicalIF":4.2,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/8d/18/TMYC_14_2165186.PMC10161953.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9784071","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 3

Fungal taxonomy: current status and research agendas for the interdisciplinary and globalisation era. 真菌分类学:跨学科和全球化时代的现状和研究议程。

IF 4.2 2区生物学

Mycology Pub Date : 2023-01-01 DOI: 10.1080/21501203.2022.2103194

Li-Wei Zhou, Tom W May

引用次数: 4

Proposal of a new family Pseudodiploösporeaceae fam. nov. (Hypocreales) based on phylogeny of Diploöspora longispora and Paecilomyces penicillatus. 新家庭的提议Pseudodiploösporeaceae fam。11 .(下creales)基于Diploöspora longispora和青霉拟青霉的系统发育。

IF 4.2 2区生物学

Mycology Pub Date : 2023-01-01 DOI: 10.1080/21501203.2022.2143919

Jingzu Sun, Shuang Yu, Yongzhong Lu, Hongwei Liu, Xingzhong Liu

{"title":"Proposal of a new family Pseudodiploösporeaceae fam. nov. (Hypocreales) based on phylogeny of Diploöspora longispora and Paecilomyces penicillatus.","authors":"Jingzu Sun, Shuang Yu, Yongzhong Lu, Hongwei Liu, Xingzhong Liu","doi":"10.1080/21501203.2022.2143919","DOIUrl":"https://doi.org/10.1080/21501203.2022.2143919","url":null,"abstract":"During a field survey of cultivated Morchella mushroom diseases, Diploöspora longispora and Paecilomyces penicillatus, causal agents of pileus rot or white mould disease were detected, which resulted in up to 80% of yield losses. Multi-locus phylogenic analysis revealed that the fungi were affiliated in a distinct clade in Hypocreales. We further constructed a phylogenetic tree with broader sampling in Hypocreales and estimated the divergence times. The D. longispora and P. penicillatus clades were estimated to have diverged from Hypocreaceae around 129 MYA and Pseudodiploösporeaceae fam. nov is herein proposed to accommodate species in this clade. Two new genera, i.e. Pseudodiploöspora and Zelopaecilomyceswere, were introduced based on morphological characteristics and phylogenic relationships of Diploöspora longispora and Paecilomyces penicillatus, respectively. Five new combinations - Pseudodiploöspora cubensis, P. longispora, P. fungicola, P. zinniae, and Zelopaecilomyces penicillatus - were proposed.","PeriodicalId":18833,"journal":{"name":"Mycology","volume":"14 1","pages":"60-73"},"PeriodicalIF":4.2,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9930807/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9314924","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 4

Applying early divergent characters in higher rank taxonomy of Melampsorineae (Basidiomycota, Pucciniales). 早期分化性状在黑霉菌科(担子菌科，担子菌科)高级分类中的应用。

IF 4.2 2区生物学

Mycology Pub Date : 2023-01-01 DOI: 10.1080/21501203.2022.2089262

Peng Zhao, Yan Li, Yuanjie Li, Fang Liu, Junmin Liang, Xin Zhou, Lei Cai

{"title":"Applying early divergent characters in higher rank taxonomy of Melampsorineae (Basidiomycota, Pucciniales).","authors":"Peng Zhao, Yan Li, Yuanjie Li, Fang Liu, Junmin Liang, Xin Zhou, Lei Cai","doi":"10.1080/21501203.2022.2089262","DOIUrl":"https://doi.org/10.1080/21501203.2022.2089262","url":null,"abstract":"Rust fungi in the order Pucciniales represent one of the largest groups of phytopathogens, which occur on mosses, ferns to advanced monocots and dicots. Seven suborders and 18 families have been reported so far, however recent phylogenetic studies have revealed para- or polyphyly of several morphologically defined suborders and families, particularly in Melampsorineae. In this study, a comprehensive phylogenetic framework was constructed based on a molecular phylogeny inferred from rDNA sequences of 160 species belonging to 16 genera in Melampsorineae (i.e. Chrysomyxa, Cerospora, Coleopuccinia, Coleosporium, Cronartium, Hylospora, Melampsora, Melampsorella, Melampsoridium, Milesina, Naohidemyces, Pucciniastrum, Quasipucciniastrum, Rossmanomyces, Thekopsora, Uredinopsis). Our phylogenetic inference indicated that 13 genera are monophyletic with strong supports, while Pucciniastrum is apparently polyphyletic. A new genus, Nothopucciniastrum was therefore established and segregated from Pucciniastrum, with ten new combinations proposed. At the family level, this study further demonstrates the importance of applying morphologies of spore-producing structures (basidia, spermogonia, aecia, uredinia and telia) in higher rank taxonomy, while those traditionally applied spore morphologies (basidiospores, spermatia, aeciospores, urediniospores and teliospores) represent later diverged characters that are more suitable for the taxonomy at generic and species levels. Three new families, Hyalopsoraceae, Nothopucciniastraceae and Thekopsoraceae were proposed based on phylogenetic and morphological distinctions, towards a further revision of Pucciniales in line with the phylogenetic relationships.","PeriodicalId":18833,"journal":{"name":"Mycology","volume":"14 1","pages":"11-36"},"PeriodicalIF":4.2,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9930778/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9329379","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Niche and ecosystem preference of earliest diverging fungi in soils. 土壤中最早分化真菌的生态位和生态系统偏好。

IF 4.2 2区生物学

Mycology Pub Date : 2023-01-01 DOI: 10.1080/21501203.2023.2237047

Jiarui Yang, Juanli Yun, Xingzhong Liu, Wenbin Du, Meichun Xiang

引用次数: 1

Development of a real-time PCR and multiplex PCR assay for the detection and identification of mycotoxigenic fungi in stored maize grains. 建立实时荧光定量PCR和多重荧光定量PCR检测和鉴定玉米储藏中产霉菌毒素真菌的方法。

IF 4.2 2区生物学

Mycology Pub Date : 2023-01-01 DOI: 10.1080/21501203.2023.2213704

Mayasar I Al-Zaban, Ahlam H Alrokban, Mohamed A Mahmoud

{"title":"Development of a real-time PCR and multiplex PCR assay for the detection and identification of mycotoxigenic fungi in stored maize grains.","authors":"Mayasar I Al-Zaban, Ahlam H Alrokban, Mohamed A Mahmoud","doi":"10.1080/21501203.2023.2213704","DOIUrl":"https://doi.org/10.1080/21501203.2023.2213704","url":null,"abstract":"This study aimed to identify important mycotoxigenic fungi and accurate detection of mycotoxin in stored maize grains using molecular methods. The current study also optimised the real-time PCR (RT-PCR) assay. The melting curve was established to identify isolated fungal species of Aspergillus (4), Fusarium (3), Penicillium (3), and Alternaria (one). A multiplex polymerase chain reaction (mPCR) technique was developed for the detection and characterisation of mycotoxin producing fungi, mycotoxin metabolic pathway genes, and the determination of eleven mycotoxins in stored maize grains using high-performance liquid chromatography (HPLC). The mPCR results indicated positive signals for potentially mycotoxigenic fungal species tested of Aspergillus, Fusarium, Penicillium, and Alternaria. A protocol for multiplex reverse transcription-polymerase chain reaction (mRT-PCR) was tested to distinguish between free and contaminated, stored maize with aflatoxin B1 (AFB1). The expression pattern of four aflatoxin biosynthetic pathway genes, AFB1 (aflQ, aflP, aflO, and aflD), was a good marker for contaminated, stored maize grains. HPLC analysis showed that maize grain samples were contaminated with mycotoxins, and the concentration was above the detection level. The results indicate that the polyphasic approach might provide a sensitive, rapid, and accurate method for detecting and identifying mycotoxigenic fungal species and mycotoxins in stored maize grains.","PeriodicalId":18833,"journal":{"name":"Mycology","volume":"14 3","pages":"227-238"},"PeriodicalIF":4.2,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/db/93/TMYC_14_2213704.PMC10424615.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10250972","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Hericium ophelieae sp. nov., a novel species of Hericium (Basidiomycota: Russulales, Hericiaceae) from the Southern Afrotemperate forests of South Africa. 南非南温带森林猴头菌属一新种(担子菌科:狐尾菌属，猴头菌科)。

IF 4.2 2区生物学

Mycology Pub Date : 2023-01-01 DOI: 10.1080/21501203.2023.2191636

B Van der Merwe, P Herrmann, Karin Jacobs

{"title":"Hericium ophelieae sp. nov., a novel species of Hericium (Basidiomycota: Russulales, Hericiaceae) from the Southern Afrotemperate forests of South Africa.","authors":"B Van der Merwe, P Herrmann, Karin Jacobs","doi":"10.1080/21501203.2023.2191636","DOIUrl":"https://doi.org/10.1080/21501203.2023.2191636","url":null,"abstract":"A novel species of Hericium was recently collected in the Afrotemperate forests (Knysna - Amatole region) of Southern Africa. The novel species shares many similar, dentate features common to other species in Hericium, and its basidiome first appears stark white and yellows with age. However, the substrate choice and gloeocystidia and basidiospore sizes of the specimens collected were distinct from other Hericium species. This was confirmed by sequencing the ITS and 28S genetic markers, respectively. The novel species is described as Hericium ophelieae sp. nov. and appears unique as it grows on hardwoods indigenous to Southern Africa. The species has larger basidiospores and wider gloeocystidia compared to its closest relative. H. ophelieae sp. nov. is the first endemic species of the medicinal mushroom genus Hericium to be described from Southern Africa, and the second to be described from Africa, after its closest relative, H. bembedjaense, which was isolated in Cameroon. Although this is the first Hericium to be described from the Southern African region, there are likely others to be discovered, and this study highlights the need for further research into the fungal diversity of Afrotemperate environments.","PeriodicalId":18833,"journal":{"name":"Mycology","volume":"14 2","pages":"133-141"},"PeriodicalIF":4.2,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10161958/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9784065","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Species diversity of pathogenic wood-rotting fungi (Agaricomycetes, Basidiomycota) in China. 中国木材腐烂致病性真菌(木链菌、担子菌)的物种多样性。

IF 4.2 2区生物学

Mycology Pub Date : 2023-01-01 DOI: 10.1080/21501203.2023.2238779

Yuan Yuan, Lu-Sen Bian, Ying-Da Wu, Jia-Jia Chen, Fang Wu, Hong-Gao Liu, Guang-Yu Zeng, Yu-Cheng Dai

{"title":"Species diversity of pathogenic wood-rotting fungi (Agaricomycetes, Basidiomycota) in China.","authors":"Yuan Yuan, Lu-Sen Bian, Ying-Da Wu, Jia-Jia Chen, Fang Wu, Hong-Gao Liu, Guang-Yu Zeng, Yu-Cheng Dai","doi":"10.1080/21501203.2023.2238779","DOIUrl":"https://doi.org/10.1080/21501203.2023.2238779","url":null,"abstract":"Wood-rotting basidiomycetes have been investigated in the Chinese forest ecosystem for the past 30 years. Two hundred and five pathogenic wood-decayers belonging to 9 orders, 30 families, and 74 genera have been found in Chinese native forests, plantations, and gardens. Seventy-two species (accounting for 35% of the total pathogenic species) are reported as pathogenic fungi in China for the first time. Among these pathogens, 184 species are polypores, nine are corticioid fungi, eight are agarics and five are hydnoid basidiomycetes. One hundred and seventy-seven species (accounting for 86%) cause white rot, while 28 species (accounting for 14%) result in brown rot; 157 species grow on angiosperm trees (accounting for 76.5%) and 44 species occur on gymnosperm trees (accounting for 21.5%), only four species inhabit both angiosperms and gymnosperms (accounting for 2%); 95 species are distributed in boreal to temperate forests and 110 in subtropical to tropical forests. In addition, 17 species, including Fomitopsis pinicola, Heterobasidion parviporum, and Phellinidium weirii etc. which were previously treated as pathogenic species in China, do not occur in China according to recent studies. In this paper, the host(s), type of forest, rot type, and distribution of each pathogenic species in China are given.","PeriodicalId":18833,"journal":{"name":"Mycology","volume":"14 3","pages":"204-226"},"PeriodicalIF":4.2,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10424591/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10250971","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Four new species of Phyllosticta from China based on morphological and phylogenetic characterization. 标题基于形态和系统发育特征的中国毛竹属四新种。

IF 4.2 2区生物学

Mycology Pub Date : 2023-01-01 DOI: 10.1080/21501203.2023.2225552

Xiao-Nan Sui, Mei-Jun Guo, Hao Zhou, Cheng-Lin Hou

引用次数: 0