Mycology最新文献_第5页

Screening for cellulases and preliminary optimisation of glucose tolerant β-glucosidase production and characterisation. 纤维素酶的筛选和葡萄糖耐受性β-葡萄糖苷酶的初步优化生产和表征。

IF 4.2 2区生物学

Mycology Pub Date : 2023-01-01 DOI: 10.1080/21501203.2022.2155261

Nivisti Singh, Bruce Sithole, Roshini Govinden

{"title":"Screening for cellulases and preliminary optimisation of glucose tolerant β-glucosidase production and characterisation.","authors":"Nivisti Singh, Bruce Sithole, Roshini Govinden","doi":"10.1080/21501203.2022.2155261","DOIUrl":"https://doi.org/10.1080/21501203.2022.2155261","url":null,"abstract":"The search for a novel microbial producer of cellulases including a glucose tolerant β-glucosidase is a challenge as most are inhibited by their product glucose. This study aims to screen for cellulolytic fungi using qualitative and quantitative screening methods. Primary screening revealed 34 of 46 fungal isolates with β-glucosidase activity. Eleven and 13 of these also displayed endoglucanase and exoglucanase activities, respectively. During secondary screening, this number was reduced to 26 β-glucosidase producers with 13 also having endoglucanase and exoglucanase activities. Isolate C1 displayed enhanced production of β-glucosidases in the presence of 0.05 M glucose (69% higher activity). Optimisation of growth conditions for β-glucosidase production by one variable at a time experiments improved production for (isolates) PS1 (64%), MB5 (84%), and C2 (69%). Isolate PS1 identified as Chaetomella sp. BBA70074 displayed the highest tolerance to glucose, retaining 10% of β-glucosidase activity in the presence of 0.8 M glucose. Tolerance to glucose increased to 14% when produced under optimal conditions. β-Glucosidase had a molecular weight of 170 kDa with a pH and temperature optima of 6 and 70°C, respectively. Future studies will include optimisation of the production of the glucose tolerant enzyme by Chaetomella sp. BBA70074.","PeriodicalId":18833,"journal":{"name":"Mycology","volume":"14 2","pages":"91-107"},"PeriodicalIF":4.2,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10161942/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9485713","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 3

A critical role of farnesol in the modulation of Amphotericin B and Aureobasidin A antifungal drug susceptibility. 法尼醇在两性霉素B和Aureobasidin A抗真菌药物敏感性调节中的关键作用。

IF 4.2 2区生物学

Mycology Pub Date : 2022-10-28 eCollection Date: 2022-01-01 DOI: 10.1080/21501203.2022.2138599

Venkatramanan Mahendrarajan, Vinay Kumar Bari

{"title":"A critical role of farnesol in the modulation of Amphotericin B and Aureobasidin A antifungal drug susceptibility.","authors":"Venkatramanan Mahendrarajan, Vinay Kumar Bari","doi":"10.1080/21501203.2022.2138599","DOIUrl":"https://doi.org/10.1080/21501203.2022.2138599","url":null,"abstract":"Candida albicans and its related species can cause opportunistic infections such as \"candidiasis\" in immunocompromised individuals with a high morbidity and mortality rate. Several antifungal drugs available in the market are often used to treat infections caused by pathogenic fungi. However, in fungi, the development of resistance against these drugs quickly evolved. Candida is a dimorphic fungus that can switch between yeast to hyphae form, requires an active biosynthesis of membrane constituents. Sphingolipid and ergosterol molecules, are the major fungal plasma membrane components, and their interaction with the antifungal drug can modulate drug susceptibility. A lipophilic compound farnesol acts as a quorum-sensing molecule synthesised by the isoprenoid biosynthesis pathway in the fungal pathogen Candida. Farnesol is secreted in a cell density-dependent manner inhibits hyphae germination and biofilm formation. In this study, we have investigated whether the farnesol molecules affect the drug susceptibility of the antifungal drug Amphotericin B (AmB) which mainly binds with ergosterol, and Aureobasidin A (AbA), a complex sphingolipid biosynthesis inhibitor. Our studies revealed that a non-toxic and low concentration of farnesol can reduce the efficacy of AmB and AbA on yeast cells. This reduction is probably through the alteration in the complex sphingolipid biosynthesis and ATP-binding cassette (ABC) type membrane transport activity. These findings may shed light on a new direction to explore the role of lipid molecules in the antifungal drug resistance mechanisms in pathogenic yeast.","PeriodicalId":18833,"journal":{"name":"Mycology","volume":"13 4","pages":"305-317"},"PeriodicalIF":4.2,"publicationDate":"2022-10-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9673792/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40485814","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 4

Mycobiota and mycotoxin-producing fungi in southern California: their colonisation and in vitro interactions. 南加州的菌群和产生霉菌毒素的真菌:它们的定植和体外相互作用。

IF 4.2 2区生物学

Mycology Pub Date : 2022-07-27 eCollection Date: 2022-01-01 DOI: 10.1080/21501203.2022.2104950

Paris Chakravarty

{"title":"Mycobiota and mycotoxin-producing fungi in southern California: their colonisation and in vitro interactions.","authors":"Paris Chakravarty","doi":"10.1080/21501203.2022.2104950","DOIUrl":"https://doi.org/10.1080/21501203.2022.2104950","url":null,"abstract":"Fungal contamination in water-damaged houses has become a major concern because of their potential health effects. During our survey in 100 water-damaged houses in southern California, we have reported 42 outdoor and 14 indoor fungal species throughout the year. Six commonly occurring indoor fungal species are Alternaria alternata, Aspergillus niger, Chaetomium globosum, Cladosporium herbarum, Penicillium chrysogenum and Stachybotrys chartarum. In the damp building materials, S. chartarum was found to be associated with A. niger, C. globosum and P. chrysogenum but not with A. alternata and C. herbarum. Stachybotrys chartarum showed strong antagonistic effect against A. alternata and C. herbarum and significantly inhibited in vitro growth of A. alternata and C. herbarum but had no effect on A. niger, C. globosum and P. chrysogenum. Two trichothecenes, produced by Stachybotrys sp., trichodermin and trichodermol, significantly inhibited spore germination and in vitro growth of A. alternata and C. herbarum but had no effect on A. niger, C. globosum, P. chrysogenum and S. chartarum. In the damp building materials (drywall, ceiling tile, and oak wood), S. chartarum significantly inhibited the growth of A. alternata and C. herbarum and had no effect on A. niger, C. globosum and P. chrysogenum in these substrata.","PeriodicalId":18833,"journal":{"name":"Mycology","volume":"13 4","pages":"293-304"},"PeriodicalIF":4.2,"publicationDate":"2022-07-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9673783/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40485810","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

New insights into the genus Gyroporus (Gyroporaceae, Boletales), with establishment of four new sections and description of five new species from China. 对石龙子属（石龙子科，Boletales）的新认识，建立了四个新部分，并描述了来自中国的五个新种。

IF 4.6 2区生物学

Mycology Pub Date : 2022-07-05 eCollection Date: 2022-01-01 DOI: 10.1080/21501203.2022.2094012

Ming Zhang, De-Chun Xie, Chao-Qun Wang, Wang Qiu Deng, Tai-Hui Li

{"title":"New insights into the genus Gyroporus (Gyroporaceae, Boletales), with establishment of four new sections and description of five new species from China.","authors":"Ming Zhang, De-Chun Xie, Chao-Qun Wang, Wang Qiu Deng, Tai-Hui Li","doi":"10.1080/21501203.2022.2094012","DOIUrl":"10.1080/21501203.2022.2094012","url":null,"abstract":"Species of Gyroporus from southern China were studied in this study. Based on morphology and molecular phylogenetic analyses of DNA sequences from the nuclear ribosomal internal transcribed spacer (ITS), the nuclear ribosomal large subunit (nrLSU), and the mitochondrial adenosine triphosphate ATP synthase subunit 6 (atp6), Gyroporus was divided into four main branches in the phylogenetic tree, and four sections were firstly proposed i.e. Gyroporus sect. Castaneus, G. sect. Cyanescens, G. sect. Longicystidiatus and G. sect. Pallidus. Five new species, i.e. G. alboluteus, G. atrocyanescens, G. pseudolongicystidiatus, G. pallidus and G. subcaerulescens, were revealed from China, and their phylogenetic positions were also analysed. Among them, G. alboluteus and G. pallidus were nested into the sect. Pallidus, although morphologically similar to G. castaneus; G. atrocyanescens and G. subcaerulescens, with obvious cyanescent oxidation reactions, were nested into the sect. Cyanescens; and G. pseudolongicystidiatus characterised by its long cystidia and was nested into the sect. Longicystidiatus. The new species were formally described and illustrated in the present study, and a key to the sections and species of Gyroporus in China was provided.","PeriodicalId":18833,"journal":{"name":"Mycology","volume":"13 3","pages":"223-242"},"PeriodicalIF":4.6,"publicationDate":"2022-07-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9354645/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40609163","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Correction notice. 更正通知。

IF 4.2 2区生物学

Mycology Pub Date : 2022-06-22 eCollection Date: 2022-01-01 DOI: 10.1080/21501203.2022.2073697

引用次数: 0

Isolation, screening, preliminary optimisation and characterisation of thermostable xylanase production under submerged fermentation by fungi in Durban, South Africa. 南非德班地区真菌深层发酵产热稳定木聚糖酶的分离、筛选、初步优化和特性研究。

IF 4.2 2区生物学

Mycology Pub Date : 2022-06-20 eCollection Date: 2022-01-01 DOI: 10.1080/21501203.2022.2079745

Priyashini Dhaver, Brett Pletschke, Bruce Sithole, Roshini Govinden

{"title":"Isolation, screening, preliminary optimisation and characterisation of thermostable xylanase production under submerged fermentation by fungi in Durban, South Africa.","authors":"Priyashini Dhaver, Brett Pletschke, Bruce Sithole, Roshini Govinden","doi":"10.1080/21501203.2022.2079745","DOIUrl":"https://doi.org/10.1080/21501203.2022.2079745","url":null,"abstract":"Fungi are renowned for their ability to produce extracellular enzymes into their surrounding environment. Xylanases are hydrolytic enzymes capable of xylan degradation. The objectives of this study were to isolate, screen for potential xylanolytic fungi from soil and tree bark samples from three locations in South Africa and to determine their growth conditions for maximum xylanase production. Forty-six isolates were obtained based on clearing zone formation on xylan-enriched agar plates using Congo red indicator. Xylanase activity was quantified during submerged fermentation. Isolate MS5, identified as Trichoderma harzianum with the highest enzyme activity (38.17 U/ml) was selected for further studies based on thermophilic properties (70°C) and pH (5.0). The culture conditions; incubation period (5 days), agitation speed (160 rpm) wheat bran (1%) and ammonium sulphate (1.2%) were optimised further. Biochemical characterisation of the crude enzyme revealed two pH and temperature optima (pH 6.0 at 60°C and 70°C, pH 8.0 at 55°C and 75°C). The enzyme retained >70% activity after 4 h at pH 6.0 at 70°C. SDS-PAGE revealed multiple protein bands with a prominent band at 70 kDa. Substrate Native PAGE revealed multiple isoforms between 55 and 130 kDa. This enzyme will be beneficial for applications in the animal feed and biofuels industries.","PeriodicalId":18833,"journal":{"name":"Mycology","volume":"13 4","pages":"271-292"},"PeriodicalIF":4.2,"publicationDate":"2022-06-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9673795/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40485811","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 9

What is the correct scientific name for "Fuling" medicinal mushroom? “涪陵”药菇的正确学名是什么?

IF 4.2 2区生物学

Mycology Pub Date : 2022-06-20 eCollection Date: 2022-01-01 DOI: 10.1080/21501203.2022.2089755

Viktor Papp, Yu-Cheng Dai

引用次数: 1

Preparation, structural characterisation, and antioxidant activities of polysaccharides from eight boletes (Boletales) in tropical China. 中国热带地区八种牛肝菌多糖的制备、结构特征和抗氧化活性。

IF 4.6 2区生物学

Mycology Pub Date : 2022-05-06 eCollection Date: 2022-01-01 DOI: 10.1080/21501203.2022.2069172

Run Tian, Hui Chai, Jun-Qiang Qiu, Zhi-Qun Liang, Hui-Jing Xie, Yong Wang, Nian-Kai Zeng

{"title":"Preparation, structural characterisation, and antioxidant activities of polysaccharides from eight boletes (Boletales) in tropical China.","authors":"Run Tian, Hui Chai, Jun-Qiang Qiu, Zhi-Qun Liang, Hui-Jing Xie, Yong Wang, Nian-Kai Zeng","doi":"10.1080/21501203.2022.2069172","DOIUrl":"10.1080/21501203.2022.2069172","url":null,"abstract":"Polysaccharides in boletes (Boletales) are economically significant to both function food and medicinal industries. The polysaccharides were extracted from the fruit bodies of eight boletes, namely, Aureoboletus longicollis, Butyriboletus hainanensis, Crocinoboletus rufoaureus, Hemioporus japonicus, Neoboletus infuscatus, Neoboletus obscureumbrinus, Tylopilus otsuensis, Xanthoconium fusciceps, which were collected from tropical China; their physicochemical properties and antioxidant activities were characterised and evaluated, respectively. The results revealed that the polysaccharides among the eight boletes were mainly composed of glucose, mannose, and galactose, with a broad molecular weight range, and contained a pyranose ring revealed by FT-IR and NMR spectral analyses. Many factors such as different species of boletes, geographic conditions, molecular weight, configuration, and monosaccharide content may affect the antioxidant power of polysaccharides, simultaneously, instead of one single factor. The antioxidant activities of the polysaccharides were measured according to in vitro assays of DPPH scavenging, superoxide anion scavenging, and ferrous ion reducing tests. The polysaccharide of C. rufoaureus has greatly superior antioxidant activity and it could serve as potential functional food or medicine.","PeriodicalId":18833,"journal":{"name":"Mycology","volume":"13 3","pages":"195-206"},"PeriodicalIF":4.6,"publicationDate":"2022-05-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9354634/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40609162","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Novel indole diketopiperazine stereoisomers from a marine-derived fungus Aspergillus sp. 海洋源真菌曲霉中的新型吲哚二酮哌嗪立体异构体

IF 4.6 2区生物学

Mycology Pub Date : 2022-04-29 eCollection Date: 2023-01-01 DOI: 10.1080/21501203.2022.2069173

Xinyang Li, Jinzhong Xu, Pinmei Wang, Wanjing Ding

引用次数: 0

Lignicolous freshwater fungi in Yunnan Province, China: an overview 云南木质素淡水真菌研究进展

IF 4.2 2区生物学

Mycology Pub Date : 2022-04-03 DOI: 10.1080/21501203.2022.2058638

Hongyi Shen, D. Bao, D. J. Bhat, H. Su, Zong-Long Luo

引用次数: 5