Antimicrobial properties and biotransforming ability of fungal endophytes from Ficus carica L. (Moraceae).

IF 4.6 2区 生物学 Q1 MYCOLOGY
Melisa Isabel Barolo, María Victoria Castelli, Silvia Noelí López
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引用次数: 1

Abstract

The endophytic fungal community associated with leaves of Ficus carica L. (Moraceae) from Argentina was investigated. Fifteen fungal isolates were isolated and identified by molecular methods into the genera Alternaria, Cladosporium, Curvularia, Diaporthe, Epicoccum, Myrothecium, Neofusicoccum, Nigrospora, Preussia and Ustilago. Cladosporium cladosporioides and Curvularia lunata were the most frequently isolated species. The fungal metabolic profiles were obtained by automated TLC and NMR and analysed by PC Analysis. Antifungal and antibacterial activity was assessed by bioautographic assays. In addition, the biotransforming ability of the fungal isolates was tested on F. carica extracts. Five isolates (33.3%) exhibited inhibitory activity against at least one of the microorganisms tested. Most of the fungal endophytes were able to metabolise the flavonoid rutin 1, and the coumarin psoralen 3 present in F. carica extracts. Further investigations of the psoralen biotransforming ability performed by the selected endophyte Alternaria alternata F8 showed the accumulation of the 6,7-furan-hydrocoumaric acid derivative 4 as the main biotransformation product. Our results corroborate that F. carica can live symbiotically with rich and diverse endophytic communities adding insights about their ecological interactions.

桑科无花果内生真菌的抗菌特性和生物转化能力。
对阿根廷桑科无花果叶片内生真菌群落进行了研究。通过分子方法分离鉴定了15株真菌,分别为Alternaria、Cladosporium、Curvularia、Diaporthe、Epicoccum、Myrothecium、Neofusicoccum、Nigrospora、Preussia和Ustilago。枝孢霉(Cladosporium cladosporioides)和弯孢霉(Curvularia lunata)是最常见的分离种。通过自动薄层色谱和核磁共振获得真菌代谢谱,并进行PC分析。用生物自显影法测定其抗真菌和抗菌活性。此外,还测试了分离的真菌对carica提取物的生物转化能力。5个分离株(33.3%)对至少一种微生物表现出抑制活性。大多数真菌内生菌能够代谢牛蒡提取物中的类黄酮芦丁1和香豆素补骨脂素3。对所选内生菌alternnaria alternata F8补骨脂素生物转化能力的进一步研究表明,6,7-呋喃-氢香豆酸衍生物4是主要的生物转化产物。本研究结果证实了船形假丝虫可以与丰富多样的内生菌群落共生,并进一步揭示了它们之间的生态相互作用。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Mycology
Mycology Medicine-Infectious Diseases
CiteScore
9.10
自引率
0.00%
发文量
18
审稿时长
13 weeks
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