Nanoscale Advances最新文献

{"title":"Reduction-responsive immobilised and protected enzymes†","authors":"Congyu Wu, Seyed Amirabbas Nazemi, Natascha Santacroce, Jenny A. Sahlin, Laura Suter-Dick and Patrick Shahgaldian","doi":"10.1039/D4NA00580E","DOIUrl":"10.1039/D4NA00580E","url":null,"abstract":"<p >We report a synthetic strategy to produce nano-immobilised and organosilica-shielded enzymes of which the biocatalytic activity is, by design, chemically enhanced under reductive conditions. The enzymes were immobilised onto silica nanoparticles through a reduction-responsive crosslinker and further shielded in an organosilica layer of controlled thickness. Under reducing conditions, disulphide bonds linking the protein to the carrier material were reduced, triggering enzyme activation. The organosilica shield prevents the enzymes from leaching from the nanobiocatalysts and preserves their integrity.</p>","PeriodicalId":18806,"journal":{"name":"Nanoscale Advances","volume":" 1","pages":" 89-93"},"PeriodicalIF":4.6,"publicationDate":"2024-11-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11603382/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142770391","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"材料科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"<i>In vitro</i> β-catenin attenuation by a mefloquine-loaded core-shell nano emulsion strategy to suppress liver cancer cells.","authors":"Priyadarshini Mohapatra, Natarajan Chandrasekaran","doi":"10.1039/d4na00547c","DOIUrl":"10.1039/d4na00547c","url":null,"abstract":"<p><p>Liver cancer, with its robust metastatic propensity, imposes a substantial global health burden of around 800 000 new cases annually. Mutations in the Wnt/β-catenin pathway genes are common in liver cancer, driving over 80% of cases. Targeting this pathway could potentially lead to better treatments. The aim of the present study was to develop a novel strategy for targeting the Wnt/β-catenin pathway while blocking the growth and division, of liver cancer cells and downregulating gene expression. This was achieved by formulating a repurposed drug (mefloquine)-loaded garlic nano-emulsion (GNE) with gold nanoparticles (GNPs) as a core-shell nano-emulsion (MQ/GNE-GNP). The biocompatible core-shell nano-emulsion (MQ/GNE-GNP) exhibited a size distribution in the range of 50-100 nm, high stability, excellent hydrophilicity, good biosafety, and sustained release. Human liver cancer cells were exposed to MQ/GNE, GNPs, and MQ/GNE-GNP at varying concentrations, and the effects were assessed through analysis of the cytotoxicity, reactive oxygen species, cell death, cell cycle analysis, and gene expression studies. It was found that MQ/GNE-GNP arrested HepG2 cells in the sub G0/G1phase and induced apoptosis. The anticancer efficacy of the core-shell nano-emulsion (MQ/GNE-GNP) resulted in higher cell death in the AO/PI staining studies, demonstrating its greater anticancer efficacy. The administration of MQ/GNE-GNP downregulated the overall expression of nuclear β-catenin, thereby suppressing the Wnt/β-catenin pathway. The protein expression level of Wnt 1 was upregulated, while β-catenin expression was significantly decreased. The core-shell nano-emulsion, incorporating a repurposed drug, could disrupt the β-catenin connections in the Wnt/β-catenin pathway. In conclusion, MQ/GNE-GNP could be a promising core-shell nano emulsion for the effective treatment of liver cancer by targeting the Wnt/β-catenin pathway.</p>","PeriodicalId":18806,"journal":{"name":"Nanoscale Advances","volume":" ","pages":"748-765"},"PeriodicalIF":4.6,"publicationDate":"2024-11-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11601157/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142750959","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"材料科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Self-assembled rosette nanotubes from tetra guanine-cytosine modules†","authors":"Usha D. Hemraz, Takeshi Yamazaki, Mounir El-Bakkari, Jae-Young Cho and Hicham Fenniri","doi":"10.1039/D4NA00567H","DOIUrl":"10.1039/D4NA00567H","url":null,"abstract":"<p >Self-assembly of small molecules into supramolecular architectures is a sustainable alternative to new advanced material design. Herein, the design and synthesis of a self-assembling system containing four covalently linked hybrid guanine and cytosine (G∧C) units that were connected through bifunctional amines are reported. These tetra G∧C motifs were characterized and self-assembled in water and methanol to produce discrete nanostructures. Each module has 24 sites for intermolecular hydrogen bonding and it is proposed that in solution the four G∧C units per molecule align into a linear stack which in turn self-assembles into a hexameric super-helix held together by 72 intermolecular hydrogen bonds. Stacking of these nano-helices led to the formation of quad rosette nanotubes.</p>","PeriodicalId":18806,"journal":{"name":"Nanoscale Advances","volume":" 1","pages":" 281-287"},"PeriodicalIF":4.6,"publicationDate":"2024-11-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11600576/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142751067","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"材料科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Layer-dependent Schottky contact at TaX₂-BY (X = S, Se, Te; Y = P, As, Sb) van der Waals interfaces.","authors":"Israr Ul Haq, A Mustaqeem, B Ali, M Umair Ashraf, U Khan, Muhammad Idrees, M Shafiq, Yousef Mohammed Alanazi, B Amin","doi":"10.1039/d4na00688g","DOIUrl":"10.1039/d4na00688g","url":null,"abstract":"<p><p>The mechanical, thermal and dynamical stabilities, electronic structure, contact type, and height of the barrier at the interface of TaX₂ (X = S, Se, Te) and BY (Y = P, As, Sb) metal-semiconductor (MS) contact are investigated <i>via</i> first principles calculations. Binding energies, mechanical properties, phonon spectra and <i>ab initio</i> molecular dynamics (AIMD) simulations confirm the stabilities of these systems. TaX₂-BY (X = S, Se, Te; Y = P, As, Sb) MS van der Waals heterostructures (vdWHs) are found to be metal with a Schottky contact at the interface. Formation of the n-type Schottky contact at the interface of TaX₂-BY (X = S, Se, Te; Y = P, As, Sb) MS vdWHs favors electron conduction over hole conduction. Small (higher) effective mass (carrier mobility) make TaS₂-BSb, TaSe₂-BSb and TaTe₂-BSb MS vdWHs, potential candidates for high speed nanoelectronic applications. Bader charge analysis shows that at the interface of TaX₂-BY (X = S, Se, Te; Y = P, As, Sb) MS vdWHs, in TaX₂ (BP, BAs) the electrons transfer from the TaX₂ layer to the BP and BAs layer, while in TaX₂ (BSb) the electrons transfer from the BSb layer to TaX₂ layer.</p>","PeriodicalId":18806,"journal":{"name":"Nanoscale Advances","volume":" ","pages":"808-818"},"PeriodicalIF":4.6,"publicationDate":"2024-11-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11638763/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142829435","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"材料科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Recent advances in irradiation-mediated synthesis and tailoring of inorganic nanomaterials for photo-/electrocatalysis","authors":"Shoushuang Huang, Can Yue, Kajsa Uvdal and Zhangjun Hu","doi":"10.1039/D4NA00806E","DOIUrl":"10.1039/D4NA00806E","url":null,"abstract":"<p >Photo-/electrocatalysis serves as a cornerstone in addressing global energy shortages and environmental pollution, where the development of efficient and stable catalysts is essential yet challenging. Despite extensive efforts, it's still a formidable task to develop catalysts with excellent catalytic behaviours, stability, and low cost. Because of its high precision, favorable controllability and repeatability, radiation technology has emerged as a potent and versatile strategy for the synthesis and modification of nanomaterials. Through meticulous control of irradiation parameters, including energy, fluence and ion species, various inorganic photo-/electrocatalysts can be effectively synthesized with tailored properties. It also enables the efficient adjustment of physicochemical characteristics, such as heteroatom-doping, defect generation, heterostructure construction, micro/nanostructure control, and so on, all of which are beneficial for lowering reaction energy barriers and enhancing energy conversion efficiency. This review comprehensively outlines the principles governing radiation effects on inorganic catalysts, followed by an in-depth discussion of recent advancements in irradiation-enhanced catalysts for various photo-/electrocatalytic applications, such as hydrogen and oxygen evolution reactions, oxygen reduction reactions, and photocatalytic applications. Furthermore, the challenges associated with ionizing and non-ionizing radiation are discussed and potential avenues for future development are outlined. By summarizing and articulating these innovative strategies, we aim to inspire further development of sustainable energy and environmental solutions to drive a greener future.</p>","PeriodicalId":18806,"journal":{"name":"Nanoscale Advances","volume":" 2","pages":" 384-418"},"PeriodicalIF":4.6,"publicationDate":"2024-11-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11601122/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142750960","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"材料科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Long-chain surface-modified red-emitting carbon dots as fluorescent additives for 3D printing vat-photopolymerization†","authors":"Simone Maturi, Andrea Baschieri, Erica Locatelli, Martina Buccioli, Mauro Comes Franchini and Letizia Sambri","doi":"10.1039/D4NA00617H","DOIUrl":"10.1039/D4NA00617H","url":null,"abstract":"<p >Carbon dots have recently attracted tremendous scientific attention thanks to their enhanced luminescence properties, photostability and low toxicity. In particular, red-emitting carbon dots (RCDs) are assuming increasing importance in biomedical applications, such as bio-imaging and phototherapy. At the same time, the possibility to create functional and complex objects by means of vat-photopolymerization-based three-dimensional (3D) printing techniques is continuously growing. This work describes the synthesis of long-chain surface-modified red emitting carbon dots, L-RCDs by esterification of RCDs, obtained from green reagents with a new solvothermal synthesis, and their employment as fluorescent additives in two formulations of photopolymerizable resins. The printing process proceeded smoothly in all cases, and red-emitting objects with different mechanical properties have been successfully obtained.</p>","PeriodicalId":18806,"journal":{"name":"Nanoscale Advances","volume":" 2","pages":" 448-455"},"PeriodicalIF":4.6,"publicationDate":"2024-11-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11615732/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142786183","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"材料科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Near-infrared DNA biosensors based on polysulfonate coatings for the sensitive detection of microRNAs","authors":"Xianghang Lin, Yang Yang, Wenzhang Zhu, Xiaorong He and Yunliang Liu","doi":"10.1039/D4NA00712C","DOIUrl":"10.1039/D4NA00712C","url":null,"abstract":"<p >MicroRNAs (miRNAs) play crucial roles in the regulation of immune cell differentiation and the immune response during allergic rhinitis (AR). Studies have shown that miRNA-155 is significantly upregulated in AR pathogenesis. Therefore, miRNA-155 can be used as a biomarker for AR diagnosis. Although fluorescent biosensors based on upconversion nanoparticles (UCNPs) have made significant advances in the detection of miRNAs, developing UCNPs with polymer coatings, efficient surface passivation, and DNA functionalization for hybrid sensing in biological media remains challenging. Herein, hairpin DNA1 (H1) is modified into a thin polysulfonic acid layer on UCNPs by sulfonamide bonds, and the fluorescence of the UCNPs is quenched by the fluorescence resonance energy transfer (FRET) process of BHQ3 carried by H1. When the target miRNA-155 is present, the hairpin structure of H1 is opened, allowing BHQ3 to move away from the UCNP surface, and the fluorescence of UCNP is restored. At the same time, hairpin DNA1 (H2) can combine with H1 to replace the miRNA-155 that is bound to H1 with the help of the opening stem ring structure of H1, and the replaced miRNA-155 can continue to react with H1 to amplify the fluorescence signal. Under the optimal experimental conditions, the linear range of miRNA-155 is 0.01–3 nM, with a detection limit of 1.14 pM. Furthermore, the constructed biosensor has been applied to determine miRNA-155 in serum samples, and the spiked recoveries range from 99.8% to 104.8%, which indicates that the developed assay has potential applications in monitoring allergic rhinitis or other miRNA related diseases.</p>","PeriodicalId":18806,"journal":{"name":"Nanoscale Advances","volume":" 2","pages":" 549-559"},"PeriodicalIF":4.6,"publicationDate":"2024-11-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11618855/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142801397","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"材料科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Study on the therapeutic effect and some immune factors by methotrexate modified superparamagnetic nanoparticles in rat mammary tumors†","authors":"Li Huang, Xing Zhao, Jun Zhang, Jiquan Zhang, Weike Liao, Yanhua Fan, Jintian Tang, Zhixu He, Fuping Gao and Weiwei Ouyang","doi":"10.1039/D4NA00295D","DOIUrl":"10.1039/D4NA00295D","url":null,"abstract":"<p > <em>Objective</em>: this study investigates the efficacy, immunological impact, and preliminary safety of methotrexate (MTX) modified magnetic Fe<small>₃</small>O<small>₄</small> nanoparticles in thermochemotherapy for mammary tumors in rats. <em>Methods</em>: transmission electron microscopy images revealed that the MTX-modified magnetic Fe<small>₃</small>O<small>₄</small> nanoparticles are nearly spherical, approximately 10 nm in diameter. Chemically co-precipitated PEI-modified magnetic nanoparticles were utilized for thermotherapy, while MTX-modified nanoparticles were employed for thermochemotherapy. These nanoparticles were locally injected into the Walker-256 tumor tissues of Wistar rats. The experimental design included twelve groups, encompassing various protocols of thermotherapy and thermochemotherapy at 47 °C and 42 °C, a group receiving only MTX nanoparticle chemotherapy, and several control groups. The biodistribution of residual magnetic nanoparticles was assessed in vital organs such as the heart, liver, lungs, kidneys, and brain. <em>Results</em>: demonstrated that these magnetic nanoparticles primarily accumulated in the tumor's central region and were unevenly distributed at the margins. The nanoparticles were capable of penetrating tumor cells but were more dispersed around them. Importantly, no residual magnetic nanoparticles were detected in vital organs. Significant tumor reduction and prolonged survival times were observed in the 47 °C thermochemotherapy group, the 47 °C thermotherapy group and the repeated 42 °C thermochemotherapy group. Additionally, significant increases in IL-2 and IFN-γ levels, along with a decrease in IL-4 levels, were detected in the 47 °C thermochemotherapy and 47 °C thermotherapy groups. <em>Conclusion</em>: MTX-modified Fe<small>₃</small>O<small>₄</small> magnetic nanoparticles demonstrate potential as an effective medium for thermochemotherapy. They are safe, tolerable, contribute to prolonged survival, and enhance immune functions in tumor-bearing rats without leaving residues in vital organs. These results provide a promising foundation for future cancer treatment research.</p>","PeriodicalId":18806,"journal":{"name":"Nanoscale Advances","volume":" 2","pages":" 601-613"},"PeriodicalIF":4.6,"publicationDate":"2024-11-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11622858/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142801409","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"材料科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Electrostatic adsorptive loading of ciprofloxacin and metronidazole on chitosan nanoparticles to prolong the drug delivery process with preserved antibacterial activities: formulation and characterization","authors":"Fatema Tuj Jahura, Farhana Khanam Ferdousi, Abu Hena Mostofa Kamal, Anwar Ul-Hamid, Md. Qamrul Ehsan and Mohammad Abul Hossain","doi":"10.1039/D4NA00548A","DOIUrl":"10.1039/D4NA00548A","url":null,"abstract":"<p >This study presents the formulation and evaluation of chitosan-based homogeneous nanoparticles of ciprofloxacin (CP) and metronidazole (MTZ) with improved loading efficiency to enhance the controlled release of drug within the human body as well as for the enhancement of the antibacterial activity. The drug-loaded chitosan nanoparticles (CSNPs) were prepared using deacetylated chitosan extracted from shrimp shells. The characterization of the drug-loaded CSNPs were performed by FTIR, XRD, SEM and TEM analyses. The association efficiencies of the drug-loaded CSNPs were found to be 93% ± 3% and 89% ± 3% for ciprofloxacin and metronidazole, respectively. TEM analysis confirmed the formation of homogeneous nano-sized particles of 0.1–1.0, 0.3–1.3, and 0.4–1.5 nm for CSNPs, CP-CSNPs and MTZ-CSNPs, respectively, which elucidated the adsorptive loading of the drug molecules on the surface of the chitosan nanoparticles. A comparison of the surface charges of the above nanoparticles suggested that the electrostatic adsorption dominated the drug loading process. Both of the drug-loaded CSNPs showed sustained release of drugs after an initial rapid release in the <em>in vitro</em> release kinetic studies. Thus, the adsorption of drugs on the surface of CSNPs resulted in the sustained release of the drugs from CSNPs without encapsulation. Positive results of the antibacterial activities of the CP-loaded CSNPs (CP-CSNPs) against both Gram-positive and Gram-negative bacteria were observed.</p>","PeriodicalId":18806,"journal":{"name":"Nanoscale Advances","volume":" 2","pages":" 621-633"},"PeriodicalIF":4.6,"publicationDate":"2024-11-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11626630/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142806530","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"材料科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Protein corona formation on different-shaped CdSe/CdS semiconductor nanocrystals†","authors":"Kunisato Kuroi, Yuta Kanazawa, Akane Shinaridome, Yuna Yasuda, Minkyo Jung, Chan-Gi Pack and Fumihiko Fujii","doi":"10.1039/D4NA00696H","DOIUrl":"10.1039/D4NA00696H","url":null,"abstract":"<p >Nanoparticles (NPs) have been widely studied and applied in medical and pharmaceutical fields. When NPs enter the <em>in vivo</em> environment, they are covered with protein molecules to form the so-called “protein corona”. Because NPs and proteins are comparable in size, the shape of NPs has a significant impact on NP–protein interactions. Although NPs of various shapes have been synthesized, how the shape of NPs affects the protein corona is poorly understood, and little is known about the underlying molecular mechanism. In the present study, we synthesized spherical, football-shaped, and rod-shaped semiconductor nanocrystals (SNCs) as model NPs and compared their interaction with human serum albumin (HSA) using fluorescence correlation spectroscopy, fluorescence quenching, Fourier-transform infrared spectroscopy, and thermodynamic analysis. Based on the binding enthalpy and entropy and secondary structural changes of HSA, with the help of hydrodynamic diameter changes of SNCs, we concluded that HSA adopts a conformation or orientation that is appropriate for the local curvature of SNCs. This study demonstrates the effect of NP shape on their interaction with proteins and provides a mechanistic perspective.</p>","PeriodicalId":18806,"journal":{"name":"Nanoscale Advances","volume":" 2","pages":" 560-571"},"PeriodicalIF":4.6,"publicationDate":"2024-11-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11621831/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142801401","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"材料科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}