Molecules and Cells最新文献_第9页

Microtubule function and dysfunction in the nervous system 神经系统中的微管功能和功能障碍

IF 3.7 3区生物学

Molecules and Cells Pub Date : 2024-10-01 DOI: 10.1016/j.mocell.2024.100111

Eun-Hae Jang , Harryn Choi , Eun-Mi Hur

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Simple methods to determine the dissociation constant, Kd 测定解离常数 Kd 的简单方法。

IF 3.7 3区生物学

Molecules and Cells Pub Date : 2024-10-01 DOI: 10.1016/j.mocell.2024.100112

Donghun Lee, Juwon Kim, Gwangrog Lee

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Editorial Board Members/Copyright 编委会成员/版权

IF 3.7 3区生物学

Molecules and Cells Pub Date : 2024-10-01 DOI: 10.1016/S1016-8478(24)00159-6

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C-TERMINAL DOMAIN PHOSPHATASE-LIKE 1 promotes flowering with TAF15b by repressing the floral repressor gene FLOWERING LOCUS C C-TERMINAL DOMAIN PHOSPHATASE-LIKE 1 通过抑制花抑制基因 FLOWERING LOCUS C，与 TAF15b 一起促进开花。

IF 3.7 3区生物学

Molecules and Cells Pub Date : 2024-10-01 DOI: 10.1016/j.mocell.2024.100114

Jinseul Kyung , Daesong Jeong , Hyunjoo Eom , Jeesoo Kim , Jong-Seo Kim , Ilha Lee

{"title":"C-TERMINAL DOMAIN PHOSPHATASE-LIKE 1 promotes flowering with TAF15b by repressing the floral repressor gene FLOWERING LOCUS C","authors":"Jinseul Kyung , Daesong Jeong , Hyunjoo Eom , Jeesoo Kim , Jong-Seo Kim , Ilha Lee","doi":"10.1016/j.mocell.2024.100114","DOIUrl":"10.1016/j.mocell.2024.100114","url":null,"abstract":"<div><div>Arabidopsis TATA-BINDING PROTEIN-ASSOCIATED FACTOR15b (TAF15b) is a plant-specific component of the transcription factor IID complex. TAF15b is involved in the autonomous pathway for flowering and represses the transcription of FLOWERING LOCUS C (FLC), a major floral repressor in Arabidopsis. While components of the autonomous flowering pathway have been extensively studied, scant attention has been directed toward elucidating the direct transcriptional regulators responsible for repressing FLC transcription. Here, we demonstrate that C-TERMINAL DOMAIN PHOSPHATASE-LIKE 1 (CPL1) is a physical and functional partner of TAF15b, playing a role in FLC repression. CPL1 is a protein phosphatase that dephosphorylates the C-terminal domain of RNA polymerase II (Pol II). Through the immunoprecipitation and mass spectrometry technique, we identified CPL1 as an interacting partner of TAF15b. Similar to taf15b, the cpl1 mutant showed a late-flowering phenotype caused by an increase in FLC levels. Additionally, the increase in cpl1 was correlated with the enrichment of phosphorylated Pol II in the FLC chromatin, as expected. We also discovered that CPL1 and TAF15b share additional common target genes through transcriptome analysis. These results suggest that TAF15b and CPL1 cooperatively repress transcription through the dephosphorylation of Pol II, especially at the FLC locus.</div></div>","PeriodicalId":18795,"journal":{"name":"Molecules and Cells","volume":"47 10","pages":"Article 100114"},"PeriodicalIF":3.7,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142291498","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

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Cover and caption 封面和标题

IF 3.7 3区生物学

Molecules and Cells Pub Date : 2024-10-01 DOI: 10.1016/S1016-8478(24)00157-2

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Linkage of alternative exon assembly in Drosophila TrpA1 transcripts 果蝇 TrpA1 转录本中替代外显子组装的联系。

IF 3.7 3区生物学

Molecules and Cells Pub Date : 2024-10-01 DOI: 10.1016/j.mocell.2024.100110

Eun Jo Du , MinHyuk Lee , Seon Yeong Kim , Se Hoon Park , Hye-Jung Ohk , KyeongJin Kang

{"title":"Linkage of alternative exon assembly in Drosophila TrpA1 transcripts","authors":"Eun Jo Du , MinHyuk Lee , Seon Yeong Kim , Se Hoon Park , Hye-Jung Ohk , KyeongJin Kang","doi":"10.1016/j.mocell.2024.100110","DOIUrl":"10.1016/j.mocell.2024.100110","url":null,"abstract":"<div><div>Drosophila TrpA1 (transient receptor potential ankyrin 1) transcripts are alternatively spliced at 2 distinct sites each with a choice of mutually exclusive exons. The first site determines exon1 encoding the amino terminus to produce either nucleophile-, electrophile- and noxious temperature-gated TRPA1(A) or electrophile- and innocuous warmth-gated TRPA1(B). The second site selects for exon10, resulting in TrpA1 variants with either exon10a or exon10b encoding a domain between the N-terminal ankyrin repeats and the transmembrane segments. Although unbiased assembly would generate TRPA1 with 4 different domain combinations, the functional impact of these alternative domains remains to be thoroughly examined. Here, we find that there is a relatively strong linkage in mRNA splicing between the 2 sites in the case of TrpA1(B), but not TrpA1(A), transcripts. Our semiquantitative assay, consisting of reverse transcription polymerase chain reaction and Sanger sequencing, revealed that exon10b is little coupled with TrpA1(B) transcripts, suggesting that only 3 isoforms, TRPA1(A)-exon10a [denoted as TRPA1(A)], TRPA1(A)-exon10b [TRPA1(A)10b], and TRPA1(B)-exon10a [TRPA1(B)], are present at detectable levels using our method. Interestingly, heterologously expressed TRPA1(A)10b showed elevated sensitivity to low concentrations of N-methyl maleimide, a cysteine-modifying electrophile, compared with other isoforms. Equivalent isoforms in malaria-transmitting Anopheles gambiae displayed a similar pattern of isoform-dependent N-methyl maleimide dose dependences, suggesting that the chemosensory regulation by selective domain assembly is conserved in insect TRPA1s. Thus, alternative RNA splicing of exon10 is coordinated in conjunction with the first exons, regulating chemical sensitivity of insect TRPA1s.</div></div>","PeriodicalId":18795,"journal":{"name":"Molecules and Cells","volume":"47 10","pages":"Article 100110"},"PeriodicalIF":3.7,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142291499","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

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Editorial Board Members/Copyright 编委会成员/版权

IF 3.7 3区生物学

Molecules and Cells Pub Date : 2024-09-01 DOI: 10.1016/S1016-8478(24)00142-0