Molecules and Cells最新文献

{"title":"Editorial Board Members/Copyright","authors":"","doi":"10.1016/S1016-8478(24)00088-8","DOIUrl":"https://doi.org/10.1016/S1016-8478(24)00088-8","url":null,"abstract":"","PeriodicalId":18795,"journal":{"name":"Molecules and Cells","volume":"47 4","pages":"Article 100063"},"PeriodicalIF":3.8,"publicationDate":"2024-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S1016847824000888/pdfft?md5=8424cfb38dba60fd4671abf001550a8c&pid=1-s2.0-S1016847824000888-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140649870","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Cdx1 and Gsc distinctly regulate the transcription of BMP4 target gene ventx3.2 by directly binding to the proximal promoter region in Xenopus gastrulae","authors":"Ravi Shankar Goutam ,&nbsp;Vijay Kumar ,&nbsp;Unjoo Lee ,&nbsp;Jaebong Kim","doi":"10.1016/j.mocell.2024.100058","DOIUrl":"10.1016/j.mocell.2024.100058","url":null,"abstract":"<div><p>A comprehensive regulatory network of transcription factors controls the dorsoventral patterning of the body axis in developing vertebrate embryos. Bone morphogenetic protein signaling is essential for activating the Ventx family of homeodomain transcription factors, which regulates embryonic patterning and germ layer identity during <em>Xenopus</em> gastrulation. Although Ventx1.1 and Ventx2.1 of the <em>Xenopus</em> Ventx family have been extensively investigated, Ventx3.2 remains largely understudied. Therefore, this study aimed to investigate the transcriptional regulation of <em>ventx3.2</em> during the embryonic development of <em>Xenopus</em>. We used goosecoid (Gsc) genome-wide chromatin immunoprecipitation-sequencing data to isolate and replicate the promoter region of <em>ventx3.</em>2. Serial deletion and site-directed mutagenesis were used to identify the <em>cis</em>-acting elements for Gsc and caudal type homeobox 1 (Cdx1) within the <em>ventx3.2</em> promoter. Cdx1 and Gsc differentially regulated <em>ventx3.2</em> transcription in this study. Additionally, positive <em>cis</em>-acting and negative response elements were observed for Cdx1 and Gsc, respectively, within the 5′ flanking region of the <em>ventx3.2</em> promoter<em>.</em> This result was corroborated by mapping the active Cdx1 response element (CRE) and Gsc response element (GRE). Moreover, a point mutation within the CRE and GRE completely abolished the activator and repressive activities of Cdx1 and Gsc, respectively. Furthermore, the chromatin immunoprecipitation-polymerase chain reaction confirmed the direct binding of Cdx1 and Gsc to the CRE and GRE, respectively. Inhibition of Cdx1 and Gsc activities at their respective functional regions, namely, the ventral marginal zone and dorsal marginal zone, reversed their effects on <em>ventx3.2</em> transcription. These results indicate that Cdx1 and Gsc modulate <em>ventx3.2</em> transcription in the ventral marginal zone and dorsal marginal zone by directly binding to the promoter region during <em>Xenopus</em> gastrulation.</p></div>","PeriodicalId":18795,"journal":{"name":"Molecules and Cells","volume":"47 4","pages":"Article 100058"},"PeriodicalIF":3.8,"publicationDate":"2024-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S1016847824000827/pdfft?md5=20cacf8f5dbf640799f8dae09845ee78&pid=1-s2.0-S1016847824000827-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140207213","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Molecular tools for recording and intervention of neuronal activity","authors":"Kisang Eom ,&nbsp;Jinhwan Jung ,&nbsp;Byungsoo Kim ,&nbsp;Jung Ho Hyun","doi":"10.1016/j.mocell.2024.100048","DOIUrl":"10.1016/j.mocell.2024.100048","url":null,"abstract":"<div><p>Observing the activity of neural networks is critical for the identification of learning and memory processes, as well as abnormal activities of neural circuits in disease, particularly for the purpose of tracking disease progression. Methodologies for describing the activity history of neural networks using molecular biology techniques first utilized genes expressed by active neurons, followed by the application of recently developed techniques including optogenetics and incorporation of insights garnered from other disciplines, including chemistry and physics. In this review, we will discuss ways in which molecular biological techniques used to describe the activity of neural networks have evolved along with the potential for future development.</p></div>","PeriodicalId":18795,"journal":{"name":"Molecules and Cells","volume":"47 4","pages":"Article 100048"},"PeriodicalIF":3.8,"publicationDate":"2024-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S1016847824000669/pdfft?md5=d7df7b69ae079ec66c4ada2dcece8b15&pid=1-s2.0-S1016847824000669-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140194204","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The structural basis of eukaryotic chaperonin TRiC/CCT: Action and folding","authors":"Hyunmin Kim ,&nbsp;Junsun Park ,&nbsp;Soung-Hun Roh","doi":"10.1016/j.mocell.2024.100012","DOIUrl":"10.1016/j.mocell.2024.100012","url":null,"abstract":"<div><p>Accurate folding of proteins in living cells often requires the cooperative support of molecular chaperones. Eukaryotic group II chaperonin <em>T</em>ailless complex polypeptide 1-<em>Ri</em>ng <em>C</em>omplex (TRiC) accomplishes this task by providing a folding chamber for the substrate that is regulated by an Adenosine triphosphate (ATP) hydrolysis-dependent cycle. Once delivered to and recognized by TRiC, the nascent substrate enters the folding chamber and undergoes folding and release in a stepwise manner. During the process, TRiC subunits and cochaperones such as prefoldin and phosducin-like proteins interact with the substrate to assist the overall folding process in a substrate-specific manner. Coevolution between the components is supposed to consult the binding specificity and ultimately expand the substrate repertoire assisted by the chaperone network. This review describes the TRiC chaperonin and the substrate folding process guided by the TRiC network in cooperation with cochaperones, specifically focusing on recent progress in structural analyses.</p></div>","PeriodicalId":18795,"journal":{"name":"Molecules and Cells","volume":"47 3","pages":"Article 100012"},"PeriodicalIF":3.8,"publicationDate":"2024-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S1016847824000050/pdfft?md5=7ee908f61d56c06d2b40f6158675e2fe&pid=1-s2.0-S1016847824000050-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139571096","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"“A broken heart” becomes sleepless, literally","authors":"Sun-Kyung Lee,&nbsp;Seung Hyun Kim,&nbsp;Joohong Ahnn","doi":"10.1016/j.mocell.2024.100009","DOIUrl":"10.1016/j.mocell.2024.100009","url":null,"abstract":"","PeriodicalId":18795,"journal":{"name":"Molecules and Cells","volume":"47 3","pages":"Article 100009"},"PeriodicalIF":3.8,"publicationDate":"2024-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S1016847824000025/pdfft?md5=93524ec9826afec1783fd7aae78f4844&pid=1-s2.0-S1016847824000025-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139425031","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"NK cell-activating receptor NKp46 does not participate in the development of obesity-induced inflammation and insulin resistance","authors":"Gracia Nathalie ,&nbsp;Beatriz Dal Santo Francisco Bonamichi ,&nbsp;Jieun Kim ,&nbsp;Jiwon Jeong ,&nbsp;Haneul Kang ,&nbsp;Emirrio Reinaldie Hartland ,&nbsp;Eveline Eveline ,&nbsp;Jongsoon Lee","doi":"10.1016/j.mocell.2023.100007","DOIUrl":"10.1016/j.mocell.2023.100007","url":null,"abstract":"<div><p>Recent evidence establishes a pivotal role for obesity-induced inflammation in precipitating insulin resistance and type-2 diabetes. Central to this process is the proinflammatory M1 adipose-tissue macrophages (ATMs) in epididymal white adipose tissue (eWAT). Notably, natural killer (NK) cells are a crucial regulator of ATMs since their cytokines induce ATM recruitment and M1 polarization. The importance of NK cells is shown by the strong increase in NK-cell numbers in eWAT, and by studies showing that removing and expanding NK cells respectively improve and worsen obesity-induced insulin resistance. It has been suggested that NK cells are activated by unknown ligands on obesity-stressed adipocytes that bind to NKp46 (encoded by <em>Ncr1</em>), which is an activating NK-cell receptor. This was supported by a study showing that NKp46-knockout mice have improved obesity-induced inflammation/insulin resistance. We therefore planned to use the NKp46-knockout mice to further elucidate the molecular mechanism by which NKp46 mediates eWAT NK-cell activation in obesity. We confirmed that obesity increased eWAT NKp46⁺ NK-cell numbers and NKp46 expression in wild-type mice and that NKp46-knockout ablated these responses. Unexpectedly, however, NKp46-knockout mice demonstrated insulin resistance similar to wild-type mice, as shown by fasting blood glucose/insulin levels and glucose/insulin tolerance tests. Obesity-induced increases in eWAT ATM numbers and proinflammatory gene expression were also similar. Thus, contrary to previously published results, NKp46 does not regulate obesity-induced insulin resistance. It is therefore unclear whether NKp46 participates in the development of obesity-induced inflammation and insulin resistance. This should be considered when elucidating the obesity-mediated molecular mechanisms that activate NK cells.</p></div>","PeriodicalId":18795,"journal":{"name":"Molecules and Cells","volume":"47 3","pages":"Article 100007"},"PeriodicalIF":3.8,"publicationDate":"2024-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S1016847823262573/pdfft?md5=92c51ad9b6f24b4662e602190a1658e0&pid=1-s2.0-S1016847823262573-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139194550","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Mitochondrial sirtuins: Energy dynamics and cancer metabolism","authors":"Hojun Lee,&nbsp;Haejin Yoon","doi":"10.1016/j.mocell.2024.100029","DOIUrl":"10.1016/j.mocell.2024.100029","url":null,"abstract":"<div><p>Mitochondria are pivotal for energy regulation and are linked to cancer. Mitochondrial sirtuins, (Sirtuin) SIRT3, SIRT4, and SIRT5, play crucial roles in cancer metabolism. This review explores their impact on cellular processes, with a focus on the NAD+ interplay and the modulation of their enzymatic activities. The varied roles of SIRT3, SIRT4, and SIRT5 in metabolic adaptation and cancer are outlined, emphasizing their tumor suppressor or oncogenic nature. We propose new insights into sirtuin biology, and cancer therapeutics, suggesting an integrated proteomics and metabolomics approach for a comprehensive understanding of mitochondrial sirtuins in cancer.</p></div>","PeriodicalId":18795,"journal":{"name":"Molecules and Cells","volume":"47 2","pages":"Article 100029"},"PeriodicalIF":3.8,"publicationDate":"2024-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S1016847824000347/pdfft?md5=ff336639478f1c759c4ed56f6be7fa7a&pid=1-s2.0-S1016847824000347-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139707268","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Adipose tissue macrophage heterogeneity in the single-cell genomics era","authors":"Haneul Kang,&nbsp;Jongsoon Lee","doi":"10.1016/j.mocell.2024.100031","DOIUrl":"10.1016/j.mocell.2024.100031","url":null,"abstract":"<div><p>It is now well-accepted that obesity-induced inflammation plays an important role in the development of insulin resistance and type 2 diabetes. A key source of the inflammation is the murine epididymal and human visceral adipose tissue. The current paradigm is that obesity activates multiple proinflammatory immune cell types in adipose tissue, including adipose-tissue macrophages (ATMs), T Helper 1 (Th1) T cells, and natural killer (NK) cells, while concomitantly suppressing anti-inflammatory immune cells such as T Helper 2 (Th2) T cells and regulatory T cells (Tregs). A key feature of the current paradigm is that obesity induces the anti-inflammatory M2 ATMs in lean adipose tissue to polarize into proinflammatory M1 ATMs. However, recent single-cell transcriptomics studies suggest that the story is much more complex. Here we describe the single-cell genomics technologies that have been developed recently and the emerging results from studies using these technologies. While further studies are needed, it is clear that ATMs are highly heterogeneous. Moreover, while a variety of ATM clusters with quite distinct features have been found to be expanded by obesity, none truly resemble classical M1 ATMs. It is likely that single-cell transcriptomics technology will further revolutionize the field, thereby promoting our understanding of ATMs, adipose-tissue inflammation, and insulin resistance and accelerating the development of therapies for type 2 diabetes.</p></div>","PeriodicalId":18795,"journal":{"name":"Molecules and Cells","volume":"47 2","pages":"Article 100031"},"PeriodicalIF":3.8,"publicationDate":"2024-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S1016847824000360/pdfft?md5=9d4d8b6a6fbfbd98ac4af9f33a94570c&pid=1-s2.0-S1016847824000360-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139735645","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The emerging era of multidisciplinary metabolism research","authors":"Jongsoon Lee","doi":"10.1016/j.mocell.2024.100032","DOIUrl":"10.1016/j.mocell.2024.100032","url":null,"abstract":"","PeriodicalId":18795,"journal":{"name":"Molecules and Cells","volume":"47 2","pages":"Article 100032"},"PeriodicalIF":3.8,"publicationDate":"2024-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S1016847824000372/pdfft?md5=1d018bd00770cdf1d9fd9a626afba2fa&pid=1-s2.0-S1016847824000372-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139900154","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Altered lipid metabolism as a predisposing factor for liver metastasis in MASLD","authors":"So Jung Kim ,&nbsp;Jeongeun Hyun","doi":"10.1016/j.mocell.2024.100010","DOIUrl":"10.1016/j.mocell.2024.100010","url":null,"abstract":"<div><p>Recently, the incidence of metabolic dysfunction-associated steatotic liver disease (MASLD) is increasing due to the high prevalence of metabolic conditions, such as obesity and type 2 diabetes mellitus. Steatotic liver is a hotspot for cancer metastasis in MASLD. Altered lipid metabolism, a hallmark of MASLD, remodels the tissue microenvironment, making it conducive to the growth of metastatic liver cancer. Tumors exacerbate the dysregulation of hepatic metabolism by releasing extracellular vesicles and particles into the liver. Altered lipid metabolism influences the proliferation, differentiation, and functions of immune cells, contributing to the formation of an immunosuppressive and metastasis-prone liver microenvironment in MASLD. This review discusses the mechanisms by which the steatotic liver promotes liver metastasis progression, focusing on its role in fostering an immunosuppressive microenvironment in MASLD. Furthermore, this review highlights lipid metabolism manipulation strategies for the therapeutic management of metastatic liver cancer.</p></div>","PeriodicalId":18795,"journal":{"name":"Molecules and Cells","volume":"47 2","pages":"Article 100010"},"PeriodicalIF":3.8,"publicationDate":"2024-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S1016847824000037/pdfft?md5=0f96546f4bc43b3ce064cb2fe952565d&pid=1-s2.0-S1016847824000037-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139491671","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}