Transcriptome-wide analysis for glucocorticoid receptor-mediated mRNA decay reveals various classes of target transcripts

IF 3.7 3区生物学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY

Molecules and Cells Pub Date : 2024-11-01 DOI:10.1016/j.mocell.2024.100130

Sung Ho Boo , Min-Kyung Shin , Hongseok Ha , Jae-Sung Woo , Yoon Ki Kim

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引用次数: 0

Abstract

The glucocorticoid receptor (GR) can bind to DNA or RNA, eliciting transcriptional activation/repression or rapid messenger RNA (mRNA) degradation, respectively. Although GR-mediated transcriptional regulation has been well-characterized, the molecular details of rapid mRNA degradation induced by glucocorticoids are not yet fully understood. Here, we demonstrate that glucocorticoid-induced GR-mediated mRNA decay (GMD) takes place in the nucleus and the cytoplasm, acting on pre-mRNAs and mRNAs. We also performed cross-linking and immunoprecipitation coupled with high-throughput sequencing analysis for GMD factors (GR, YBX1, and HRSP12) and mRNA sequencing analysis to identify endogenous GMD substrates. Our comprehensive coupled with high-throughput sequencing and mRNA sequencing analyses reveal that a range of cellular transcripts containing a common binding site for GR, YBX1, and HRSP12 are preferential targets for GMD, suggesting possible new functions of GMD in various biological events.

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对糖皮质激素受体介导的 mRNA 衰减进行的全转录组分析揭示了各类目标转录本。

糖皮质激素受体（GR）可与 DNA 或 RNA 结合，分别引起转录激活/抑制或 mRNA 快速降解。尽管GR介导的转录调控已被充分描述，但糖皮质激素（GC）诱导的mRNA快速降解的分子细节尚未完全清楚。在这里，我们证明了 GC 诱导的 GR 介导的 mRNA 降解（GMD）发生在细胞核和细胞质中，作用于前 mRNA 和 mRNA。我们还对 GMD 因子（GR、YBX1 和 HRSP12）进行了交联和免疫沉淀以及高通量测序（CLIP-seq）分析，并对 mRNA 进行了测序（mRNA-seq）分析，以确定内源性 GMD 底物。我们全面的CLIP-seq和mRNA测序分析表明，一系列含有GR、YBX1和HRSP12共同结合位点的细胞转录本是GMD的首选靶标，这表明GMD在各种生物事件中可能具有新的功能。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Molecules and Cells 生物-生化与分子生物学

CiteScore

6.60

自引率

10.50%

发文量

审稿时长

2.3 months

期刊介绍： Molecules and Cells is an international on-line open-access journal devoted to the advancement and dissemination of fundamental knowledge in molecular and cellular biology. It was launched in 1990 and ISO abbreviation is "Mol. Cells". Reports on a broad range of topics of general interest to molecular and cell biologists are published. It is published on the last day of each month by the Korean Society for Molecular and Cellular Biology.