Molecules and Cells最新文献

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IF 3.7 3区生物学

Molecules and Cells Pub Date : 2024-09-01 DOI: 10.1016/S1016-8478(24)00142-0

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Cover and caption

IF 3.7 3区生物学

Molecules and Cells Pub Date : 2024-09-01 DOI: 10.1016/S1016-8478(24)00140-7

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Natural allelic diversity of the calcium signaling regulators in plants 植物钙信号调节因子的天然等位基因多样性。

IF 3.7 3区生物学

Molecules and Cells Pub Date : 2024-08-02 DOI: 10.1016/j.mocell.2024.100104

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Cover and caption 封面和标题

IF 3.7 3区生物学

Molecules and Cells Pub Date : 2024-08-01 DOI: 10.1016/S1016-8478(24)00130-4

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Corrigendum to “Sizzled (Frzb3) physically interacts with noncanonical Wnt ligands to inhibit gastrulation cell movement” [Volume 47, Issue 6, June 2024, 100068. start page – end page] 更正："Sizzled（Frzb3）与非经典Wnt配体的物理相互作用抑制了胃形成细胞的移动"[第47卷第6期，2024年6月，100068. 开始页 - 结束页]。

IF 3.7 3区生物学

Molecules and Cells Pub Date : 2024-08-01 DOI: 10.1016/j.mocell.2024.100088

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Decrease of alpha-crystallin A by miR-325-3p in retinal cells under blue light exposure 在蓝光照射下，视网膜细胞中的 miR-325-3p 会降低α-晶体蛋白 A 的含量。

IF 3.7 3区生物学

Molecules and Cells Pub Date : 2024-08-01 DOI: 10.1016/j.mocell.2024.100091

{"title":"Decrease of alpha-crystallin A by miR-325-3p in retinal cells under blue light exposure","authors":"","doi":"10.1016/j.mocell.2024.100091","DOIUrl":"10.1016/j.mocell.2024.100091","url":null,"abstract":"<div>Exposure to blue light can lead to retinal degeneration, causing adverse effects on eye health. Although the loss of retinal cells due to blue light exposure has been observed, the precise molecular mechanisms underlying this process remain poorly understood. In this study, we investigate the role of alpha-crystallin A (CRYAA) in neuro-retinal degeneration and their regulation by blue light. We observed significant apoptotic cell death in both the retina of rats and the cultured neuro-retinal cells. The expressions of Cryaa mRNA and protein were significantly downregulated in the retina exposed to blue light. We identified that miR-325-3p reduces Cryaa mRNA and protein by binding to its 3′-untranslated region. Upregulation of miR-325-3p destabilized Cryaa mRNA and suppresses CRYAA, whereas downregulation of miR-325-3p increased both expressions. Blue light-induced neuro-retinal cell death was alleviated by CRYAA overexpression. These results highlight the critical role of Cryaa mRNA and miR-325-3p molecular axis in blue light-induced retinal degeneration. Consequently, targeting CRYAA and miR-325-3p presents a potential strategy for protecting against blue light-induced retinal degeneration.</div>","PeriodicalId":18795,"journal":{"name":"Molecules and Cells","volume":null,"pages":null},"PeriodicalIF":3.7,"publicationDate":"2024-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S101684782400116X/pdfft?md5=017abe8e4402241b5ce9d97d3299f88e&pid=1-s2.0-S101684782400116X-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141600618","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

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Brief guidelines for zebrafish embryotoxicity tests 斑马鱼胚胎毒性试验简要指南。

IF 3.7 3区生物学

Molecules and Cells Pub Date : 2024-08-01 DOI: 10.1016/j.mocell.2024.100090

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Editorial Board Members/Copyright 编委会成员/版权

IF 3.7 3区生物学

Molecules and Cells Pub Date : 2024-08-01 DOI: 10.1016/S1016-8478(24)00132-8

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Comparative analysis on natural variants of fire blight resistance protein FB_MR5 indicates distinct effector recognition mechanisms 火疫病抗性蛋白 FB_MR5 天然变体的比较分析表明了不同的效应识别机制。

IF 3.7 3区生物学

Molecules and Cells Pub Date : 2024-08-01 DOI: 10.1016/j.mocell.2024.100094

{"title":"Comparative analysis on natural variants of fire blight resistance protein FB_MR5 indicates distinct effector recognition mechanisms","authors":"","doi":"10.1016/j.mocell.2024.100094","DOIUrl":"10.1016/j.mocell.2024.100094","url":null,"abstract":"<div>FB_MR5 is a nucleotide-binding domain and leucine-rich repeat protein identified from wild apple species Malus × robusta 5 conferring disease resistance to bacterial fire blight. FB_MR5 (hereafter MrMR5) recognizes the cysteine protease effector EaAvrRpt2 secreted from the causal agent of bacterial fire blight, Erwinia amylovora. We previously reported that MrMR5 is activated by the C-terminal cleavage product (ACP3) of Malus domestica RIN4 (MdRIN4) produced by EaAvrRpt2-directed proteolysis. We show that MbMR5 from a wild apple species Malus baccata shares 99.4% amino acid sequence identity with MrMR5. Surprisingly, transient expression of MbMR5 in Nicotiana benthamiana showed autoactivity in contrast to MrMR5. Domain swap and mutational analyses revealed that 1 amino acid polymorphism in the MbMR5 CC domain is critical in enhancing autoactivity. We further demonstrated that MrMR5 carrying 7 amino acid polymorphisms present in MbMR5 is not activated by MdRIN4 ACP3 but recognizes AvrRpt2 without MdRIN4 in N. benthamiana. Our findings indicate that naturally occurring polymorphisms of MR5 natural variants can confer its cell death-inducing activity and the effector recognition mechanism likely due to altered compatibility with RIN4.</div>","PeriodicalId":18795,"journal":{"name":"Molecules and Cells","volume":null,"pages":null},"PeriodicalIF":3.7,"publicationDate":"2024-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S1016847824001195/pdfft?md5=fbe0e04bb859342ae475fcf9befb2246&pid=1-s2.0-S1016847824001195-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141727530","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

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Extracellular flux assay (Seahorse assay): Diverse applications in metabolic research across biological disciplines 细胞外通量测定法（海马测定法）：生物学科代谢研究中的多样化应用。

IF 3.7 3区生物学

Molecules and Cells Pub Date : 2024-08-01 DOI: 10.1016/j.mocell.2024.100095

{"title":"Extracellular flux assay (Seahorse assay): Diverse applications in metabolic research across biological disciplines","authors":"","doi":"10.1016/j.mocell.2024.100095","DOIUrl":"10.1016/j.mocell.2024.100095","url":null,"abstract":"<div>Metabolic networks are fundamental to cellular processes, driving energy production, biosynthesis, redox regulation, and cellular signaling. Recent advancements in metabolic research tools have provided unprecedented insights into cellular metabolism. Among these tools, the extracellular flux analyzer stands out for its real-time measurement of key metabolic parameters: glycolysis, mitochondrial respiration, and fatty acid oxidation, leading to its widespread use. This review provides a comprehensive summary of the basic principles and workflow of the extracellular flux assay (the Seahorse assay) and its diverse applications. We highlight the assay's versatility across various biological models, including cancer cells, immunocytes, Caenorhabditis elegans, tissues, isolated mitochondria, and three-dimensional structures such as organoids, and summarize key considerations for using extracellular flux assay in these models. Additionally, we discuss the limitations of the Seahorse assay and propose future directions for its development. This review aims to enhance the understanding of extracellular flux assay and its significance in biological studies.</div>","PeriodicalId":18795,"journal":{"name":"Molecules and Cells","volume":null,"pages":null},"PeriodicalIF":3.7,"publicationDate":"2024-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S1016847824001201/pdfft?md5=f73d489097c87d20278cdae10d915f5d&pid=1-s2.0-S1016847824001201-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141734646","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0