Brief guide to detecting ferroptosis

Abstract

Ferroptosis is an iron-dependent, lipid-peroxidation-driven form of regulated cell death that is distinct from apoptosis and necroptosis. Its involvement in various diseases highlights the need for reliable detection strategies. We provide a concise guide for ferroptosis detection, outlining key mechanisms, including iron metabolism, lipid remodeling, and antioxidant failure. Cellular, biochemical, genetic, and morphological methods, including viability assays, lipid reactive oxygen species probes, and electron microscopy, have been used to identify ferroptosis in vitro and in vivo. A multiparametric approach is emphasized to ensure the specificity and reproducibility.