Molecules and Cells最新文献_第2页

IF 3.7 3区生物学

Molecules and Cells Pub Date : 2025-05-29 DOI: 10.1016/j.mocell.2025.100234

Woojin Hong, Seokjun G. Ha, Hyunwoo C. Kwon, Seung-Jae V. Lee

引用次数: 0

Conformational flexibility of His200 enables catalytic activity in the T200H mutant of carbonic anhydrase II His200的构象灵活性使碳酸酐酶II的T200H突变体具有催化活性。

IF 3.7 3区生物学

Molecules and Cells Pub Date : 2025-05-27 DOI: 10.1016/j.mocell.2025.100226

Seon Woo Lim , Hannah Jeong , Gwang Ho Kim , Duyoung Min , Jin Kyun Kim , Chae Un Kim

{"title":"Conformational flexibility of His200 enables catalytic activity in the T200H mutant of carbonic anhydrase II","authors":"Seon Woo Lim , Hannah Jeong , Gwang Ho Kim , Duyoung Min , Jin Kyun Kim , Chae Un Kim","doi":"10.1016/j.mocell.2025.100226","DOIUrl":"10.1016/j.mocell.2025.100226","url":null,"abstract":"<div><div>Carbonic anhydrase II (CAII) is one of the most efficient enzymes known, catalyzing the reversible hydration of CO<sub>2</sub> to regulate pH and facilitate CO<sub>2</sub> transport in biological systems. Its exceptional catalytic rate depends on a highly ordered active site composed of a Zn<sup>2+</sup> ion and a hydrogen-bonded water network that supports substrate binding, proton transfer, and product release. Among the residues maintaining this network, Thr200 plays a crucial role by stabilizing key water molecules. To investigate the structural and functional consequences of perturbing this network, we examined the T200H mutant of CAII using high-pressure cryocooling and X-ray crystallography under CO<sub>2</sub> pressures of 0, 5, and 20 atm. The crystallographic snapshots captured the resting (T200H-0atm), substrate-bound (T200H-20atm), and product-bound (T200H-5atm) states of the T200H mutant. In the resting state, His200 disrupts the active site by displacing essential water molecules (W1 and W2), thereby impairing the proton transfer pathway. However, the substrate- and product-bound states reveal that His200 exhibits conformational flexibility, allowing partial restoration of the water network required for catalysis. These findings suggest that His200 functions as a dynamic gatekeeper, modulating access of water, substrate, and product to the active site. This structural plasticity explains how the T200H mutant retains partial catalytic activity despite a mutation that would otherwise severely hinder function. Our results provide new insights into active-site dynamics in CAII and offer a foundation for designing isoform-specific inhibitors or engineered carbonic anhydrase variants with tunable catalytic properties.</div></div>","PeriodicalId":18795,"journal":{"name":"Molecules and Cells","volume":"48 7","pages":"Article 100226"},"PeriodicalIF":3.7,"publicationDate":"2025-05-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144181494","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Brief guide to Caenorhabditis elegans survival assays 秀丽隐杆线虫存活测定的简要指南。

IF 3.7 3区生物学

Molecules and Cells Pub Date : 2025-05-26 DOI: 10.1016/j.mocell.2025.100232

Hyunwoo C. Kwon, Seung-Jae V. Lee

引用次数: 0

Brief guide to assays for measuring health parameters using Caenorhabditis elegans 用秀丽隐杆线虫测定健康参数的试验指南。

IF 3.7 3区生物学

Molecules and Cells Pub Date : 2025-05-26 DOI: 10.1016/j.mocell.2025.100233

Seungjae Hwang, Jongsun Lee, Seung-Jae V. Lee

引用次数: 0

Cover and caption 封面及标题

IF 3.7 3区生物学

Molecules and Cells Pub Date : 2025-05-21 DOI: 10.1016/S1016-8478(25)00052-4

引用次数: 0

Editorial Board Members/Copyright 编辑委员会成员/版权

IF 3.7 3区生物学

Molecules and Cells Pub Date : 2025-05-21 DOI: 10.1016/S1016-8478(25)00053-6

引用次数: 0

MicroRNA-196a increases apoptosis in B cells through downregulation of FOXO1 MicroRNA-196a通过下调fox01增加B细胞凋亡。

IF 3.7 3区生物学

Molecules and Cells Pub Date : 2025-05-20 DOI: 10.1016/j.mocell.2025.100223

Soyoung Kim , Mina Han , Hyun Ju Hwang , Young-Ho Ahn , Ho Joon Im , Sang-Hyun Hwang , Kyung-Nam Koh , Nayoung Kim

{"title":"MicroRNA-196a increases apoptosis in B cells through downregulation of FOXO1","authors":"Soyoung Kim , Mina Han , Hyun Ju Hwang , Young-Ho Ahn , Ho Joon Im , Sang-Hyun Hwang , Kyung-Nam Koh , Nayoung Kim","doi":"10.1016/j.mocell.2025.100223","DOIUrl":"10.1016/j.mocell.2025.100223","url":null,"abstract":"<div><div>MicroRNAs (miRNAs) are key regulators of cancer pathogenesis, and their expression is often dysregulated in cancer cells. The role of miR-196a-5p has been investigated in various types of cancers. However, it is relatively less understood in B-cell malignancies. This study aimed to investigate the role of miR-196a-5p in B cells by using a human diffuse large B-cell lymphoma cell line, SU-DHL-6, and mouse B lymphocytes. The enforced expression of miR-196a in SU-DHL-6 cells increased daunorubicin-mediated apoptosis. Luciferase assay revealed that FOXO1 was a direct target of miR-196a-5p in SU-DHL-6 cells. The mRNA and protein expression of FOXO1 was downregulated in miR-196a-overexpressing SU-DHL-6 cells. In addition, miR-196a-5p was highly expressed in mouse bone marrow cells, compared with that of splenic B cells, and FOXO1 expression was negatively correlated with miR-196a-5p level. miR-196a-5p was upregulated by B-cell receptor stimulation, which was inversely correlated with FOXO1 expression in splenic B cells. Apoptosis was increased when miR-196a-5p was upregulated in murine primary B cells. These results identify miR-196a-5p as a post-transcriptional regulator of FOXO1 and indicate its importance in regulating B-cell malignancies and activation.</div></div>","PeriodicalId":18795,"journal":{"name":"Molecules and Cells","volume":"48 7","pages":"Article 100223"},"PeriodicalIF":3.7,"publicationDate":"2025-05-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144128161","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A novel high-throughput single-molecule technique DNA curtain: Applications for DNA metabolism 一种新的高通量单分子技术DNA幕：在DNA代谢中的应用。

IF 3.7 3区生物学

Molecules and Cells Pub Date : 2025-05-20 DOI: 10.1016/j.mocell.2025.100224

Soyeong An , Youngseo Kim , Ja Yil Lee

引用次数: 0

Effects of heterozygous SMG1 mutations on nonsense-mediated mRNA decay in human pluripotent stem cell model 杂合SMG1突变对人多能干细胞模型中无义介导的mRNA衰变的影响。

IF 3.7 3区生物学

Molecules and Cells Pub Date : 2025-05-20 DOI: 10.1016/j.mocell.2025.100225

Chanyoung Lee , Jin Sook Lee , Yejin Kwon , Aeri Shin , Tae Yeong Jeong , Jiyun Yang , Jung Woo Hwang , Hyeong-In Kim , Hee-Jung Choi , Yoon Ki Kim , Murim Choi , Kyoungmi Kim , Woong Sun , Jong-Hee Chae

{"title":"Effects of heterozygous SMG1 mutations on nonsense-mediated mRNA decay in human pluripotent stem cell model","authors":"Chanyoung Lee , Jin Sook Lee , Yejin Kwon , Aeri Shin , Tae Yeong Jeong , Jiyun Yang , Jung Woo Hwang , Hyeong-In Kim , Hee-Jung Choi , Yoon Ki Kim , Murim Choi , Kyoungmi Kim , Woong Sun , Jong-Hee Chae","doi":"10.1016/j.mocell.2025.100225","DOIUrl":"10.1016/j.mocell.2025.100225","url":null,"abstract":"<div><div>Nonsense-mediated mRNA decay (NMD) eliminates transcripts containing premature termination codons, thereby preventing errors in protein synthesis. Serine/threonine-protein kinase SMG1 is a crucial kinase for NMD response, interacting with other regulatory proteins such as SMG8 and SMG9. We identified a de novo heterozygous variant in <em>SMG1</em> p.Gln2398Glu (c.7192C>G) in a patient with global developmental delay, facial dysmorphism, and oculomotor apraxia. Thus, stem cell models with <em>SMG1</em> mutations using gene editing technology were established to address the functional consequences of this mutation. While mutations causing the reduction in <em>SMG1</em> gene dosage by alterations in splicing (c.7192_7194delinsGAA; GAA/+) or frameshift (c.4331_4337del; KO/+) led to a mild but significant reduction of NMD activity, NMD activity was not altered in cells with the <em>SMG1</em> GAG/+ mutation. Furthermore, cortical organoids from hPSC<sup>GAA/+</sup> exhibited size reduction compared with the control (CTL) or GAG/+, suggesting that reduced NMD activity can affect nervous system development. These findings suggest that hypomorphic <em>SMG1</em> mutations can cause reduced NMD activity and subsequent biological responses, while the mutation found in the patient alone may not be sufficient to induce pathological symptoms.</div></div>","PeriodicalId":18795,"journal":{"name":"Molecules and Cells","volume":"48 7","pages":"Article 100225"},"PeriodicalIF":3.7,"publicationDate":"2025-05-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144128159","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

SimpleViz: A user-friendly, web-based tool for publication-ready data visualization in bioinformatics SimpleViz：一个用户友好的，基于web的工具，用于生物信息学中出版就绪的数据可视化。

IF 3.7 3区生物学

Molecules and Cells Pub Date : 2025-05-08 DOI: 10.1016/j.mocell.2025.100222

Byeong Seob Oh , Juhee Kim , Minjeong Kwon , Jiwon Bang , Kwang-Jun Lee , Eun-Jin Lee , Yong-Joon Cho

{"title":"SimpleViz: A user-friendly, web-based tool for publication-ready data visualization in bioinformatics","authors":"Byeong Seob Oh , Juhee Kim , Minjeong Kwon , Jiwon Bang , Kwang-Jun Lee , Eun-Jin Lee , Yong-Joon Cho","doi":"10.1016/j.mocell.2025.100222","DOIUrl":"10.1016/j.mocell.2025.100222","url":null,"abstract":"<div><div>Large-scale datasets are central to bioinformatics research, creating a demand for intuitive visualization tools that transform complex data into accessible graphics. Existing visualization software often comes with high costs or requires coding expertise, limiting accessibility for many researchers. To address this gap, we introduce SimpleViz, a free, web-based platform that enables the creation of professional-quality figures without the need for programming skills. SimpleViz offers gene-level analysis of RNA-seq data and core visualization types such as box/violin/dot plots, volcano plots, principal component analysis plots, and heatmaps, with extensive customization options for detailed adjustments and built-in statistical comparisons. Developed on a Shiny interface, SimpleViz simplifies the process of data upload, visualization generation, and customization, ensuring that users can produce tailored visuals suited for publication. With plans for continuous improvement based on user feedback, SimpleViz provides an adaptable, accessible solution that meets evolving data analysis needs in biomedical research.</div></div>","PeriodicalId":18795,"journal":{"name":"Molecules and Cells","volume":"48 7","pages":"Article 100222"},"PeriodicalIF":3.7,"publicationDate":"2025-05-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143971358","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0