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IF 3.7 3区生物学

Molecules and Cells Pub Date : 2025-07-18 DOI: 10.1016/S1016-8478(25)00082-2

引用次数: 0

Editorial Board Members/Copyright 编辑委员会成员/版权

IF 3.7 3区生物学

Molecules and Cells Pub Date : 2025-07-18 DOI: 10.1016/S1016-8478(25)00083-4

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Crucial roles of calcium ATPases and phosphoinositides: Insights into pathophysiology and therapeutic strategies 钙atp酶和磷酸肌苷的关键作用：病理生理学和治疗策略的见解。

IF 6.5 3区生物学

Molecules and Cells Pub Date : 2025-07-11 DOI: 10.1016/j.mocell.2025.100254

Hyun-Oh Gu , Seung Wan Noh , Ok-Hee Kim , Byung-Chul Oh

{"title":"Crucial roles of calcium ATPases and phosphoinositides: Insights into pathophysiology and therapeutic strategies","authors":"Hyun-Oh Gu , Seung Wan Noh , Ok-Hee Kim , Byung-Chul Oh","doi":"10.1016/j.mocell.2025.100254","DOIUrl":"10.1016/j.mocell.2025.100254","url":null,"abstract":"<div><div>Calcium (Ca²⁺) serves as a pivotal intracellular messenger, influencing a diverse array of cellular processes, including muscle contraction, neurotransmission, and hormone secretion. It also plays a critical role in the regulation of gene expression. Intracellular Ca²⁺ levels are stringently controlled and maintained within a narrow physiological range, primarily by plasma membrane Ca<sup>2+</sup>-ATPases, sarco-/endoplasmic reticulum Ca<sup>2+</sup>-ATPases, and secretory pathway Ca<sup>2+</sup>-ATPases. These ATPases orchestrate the influx, efflux, and sequestration of Ca²⁺ across cellular compartments, thereby ensuring cellular functionality and survival. This review delves into the intricate interplay between Ca²⁺ and phosphoinositides, essential lipid signaling molecules that modulate Ca<sup>2+</sup>-ATPase activities and link Ca²⁺ signaling to a wide range of cellular functions. By examining the molecular dynamics of Ca<sup>2+</sup>-ATPases and their regulatory interactions with phosphoinositides, we discuss their roles under both physiological and pathological conditions, highlighting how disturbances in these interactions contribute to disease. Furthermore, we explore the potential of targeting these Ca²⁺ regulatory mechanisms as a therapeutic strategy for diseases characterized by Ca²⁺ dysregulation, providing insights into future research directions and clinical applications.</div></div>","PeriodicalId":18795,"journal":{"name":"Molecules and Cells","volume":"48 9","pages":"Article 100254"},"PeriodicalIF":6.5,"publicationDate":"2025-07-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144626726","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Tumor-derived CD84 promotes growth of acute myeloid leukemia cells via regulating nonhomologous DNA end-joining pathway 肿瘤源性CD84通过调节非同源DNA末端连接途径促进急性髓系白血病细胞生长。

IF 3.7 3区生物学

Molecules and Cells Pub Date : 2025-07-07 DOI: 10.1016/j.mocell.2025.100253

Xiang Li , Bo Chen , Jiajing Zhou , Chunling Li , Yue Yu , Erdi Zhao , Xingli Wu , Changzhu Jin , Minjing Li , Jiankai Feng , Leilei Lin , Yancun Yin

{"title":"Tumor-derived CD84 promotes growth of acute myeloid leukemia cells via regulating nonhomologous DNA end-joining pathway","authors":"Xiang Li , Bo Chen , Jiajing Zhou , Chunling Li , Yue Yu , Erdi Zhao , Xingli Wu , Changzhu Jin , Minjing Li , Jiankai Feng , Leilei Lin , Yancun Yin","doi":"10.1016/j.mocell.2025.100253","DOIUrl":"10.1016/j.mocell.2025.100253","url":null,"abstract":"<div><div>CD84, a member of the signaling lymphocyte activation molecule immunoglobulin superfamily, has been identified as playing a significant role in regulating various immune cell activities. However, its intrinsic role in cancer cells remains largely unknown. We aim to explore the direct role of CD84 in acute myeloid leukemia (AML) progression and to clarify the underlying molecular mechanisms involved in nonhomologous end-joining (NHEJ) repair. Herein, we found that CD84 is frequently upregulated in various types of AML and leukemia initiation cells (LICs)-enriched cells. Knockdown or blocking of CD84 significantly inhibits the growth and induces the apoptosis of AML cells. Moreover, knockdown of <em>CD84</em> significantly delays AML progression and prolongs the survival of the xenografted mice in vivo. Mechanistically, CD84 promotes the expression of NHEJ core factors by recruiting SAP and activating the AKT signaling pathway. Knockdown of <em>CD84</em> inhibits NHEJ repair in AML cells via regulating the expression of NHEJ core factors, including <em>PRKDC</em>, <em>LIG4</em>, <em>XRCC5</em>, and <em>DCLRE1C.</em> Subsequently, this leads to double-strand breaks accumulation and cell apoptosis. Importantly, CD84 is required for the proliferation and self-renewal of human LICs. In conclusion, CD84 plays important roles in AML growth and progression through promoting NHEJ repair. Targeting CD84 may be a potential approach for inhibiting AML development and eliminating LICs.</div></div>","PeriodicalId":18795,"journal":{"name":"Molecules and Cells","volume":"48 9","pages":"Article 100253"},"PeriodicalIF":3.7,"publicationDate":"2025-07-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144601023","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Cyclotides as novel plant-derived scaffolds for orally active cyclic peptide therapeutics 环肽作为口服活性环肽治疗的新型植物源支架。

IF 3.7 3区生物学

Molecules and Cells Pub Date : 2025-07-04 DOI: 10.1016/j.mocell.2025.100252

Youbong Hyun

{"title":"Cyclotides as novel plant-derived scaffolds for orally active cyclic peptide therapeutics","authors":"Youbong Hyun","doi":"10.1016/j.mocell.2025.100252","DOIUrl":"10.1016/j.mocell.2025.100252","url":null,"abstract":"<div><div>Cyclotides are a group of plant-derived small peptides, characterized by a head-to-tail cyclic backbone and 3 conserved cysteine knots. The unique structure endows cyclotides with exceptional chemical stability and intrinsic cell-penetrating capacities, allowing them to serve as orally active agents for host defense. These properties also position cyclotides as ideal scaffolds for the design of novel edible drugs. This review provides a comprehensive overview of the discovery, distribution, and evolutionary divergence of cyclotides in flowering plants, while also discussing their classification and applications in structure-based engineering for various purposes. Additionally, recent advancements in the biological and pharmaceutical activities of both naturally occurring and synthetic “designer” cyclotides are summarized. The complexities of the cyclotide biosynthetic pathway have posed challenges for their synthesis in non-native organisms. Thus, the article concludes by exploring the latest strategies for large-scale cyclotide production in plants, highlighting <em>Viola</em> species as rich sources for future cyclotide engineering.</div></div>","PeriodicalId":18795,"journal":{"name":"Molecules and Cells","volume":"48 9","pages":"Article 100252"},"PeriodicalIF":3.7,"publicationDate":"2025-07-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144575904","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A practical guide to ordering C. elegans strains for biological research 生物学研究中秀丽隐杆线虫菌株排序实用指南。

IF 3.7 3区生物学

Molecules and Cells Pub Date : 2025-07-02 DOI: 10.1016/j.mocell.2025.100251

Yeon-Ji Park , Kyeong Min Moon , Kyuhyung Kim

引用次数: 0

N-Terminal deleted isoforms of E3 ligase RNF220 are ubiquitously expressed and required for mouse muscle differentiation E3连接酶RNF220 n端缺失异构体普遍表达，是小鼠肌肉分化所必需的。

IF 3.7 3区生物学

Molecules and Cells Pub Date : 2025-07-01 DOI: 10.1016/j.mocell.2025.100250

SeokGyeong Choi , Sojung Ha , Donald J. Wolfgeher , Jee Won Kim , Young-Hyun Go , Hyuk-Jin Cha , Gyu-Un Bae , Stephen J. Kron , Woo-Young Kim

{"title":"N-Terminal deleted isoforms of E3 ligase RNF220 are ubiquitously expressed and required for mouse muscle differentiation","authors":"SeokGyeong Choi , Sojung Ha , Donald J. Wolfgeher , Jee Won Kim , Young-Hyun Go , Hyuk-Jin Cha , Gyu-Un Bae , Stephen J. Kron , Woo-Young Kim","doi":"10.1016/j.mocell.2025.100250","DOIUrl":"10.1016/j.mocell.2025.100250","url":null,"abstract":"<div><div>Four isoform peptides of the novel E3 ligase ring finger protein 220 (RNF220) have been identified in humans. However, all of the previous studies have predominantly focused on isoform 1 (the full-length form), which consists of 566 amino acids. Here, we show that a shorter isoform, which is 308 amino acids lacking most of the N-terminus (human isoform 4; mouse isoform 3; ΔN-RNF220), is the predominant and ubiquitously expressed variant that warrants functional investigation. Both isoform 1 and ΔN-RNF220 are expressed in the brain; however, ΔN-RNF220 is the major isoform expressed in all other tissues in mice. Consistently, H3K4me3 ChIP-seq data from ENCODE reveal that the transcription start site for ΔN-RNF220 demonstrates broader and stronger activity across human tissues than that of isoform 1. ΔN-RNF220 produces 2 peptides (4a and 4b) through alternative translation initiation, with isoform 4b displaying distinct subcellular localization, subnuclear structures and interaction with a nuclear protein WDR5. Notably, during embryonic stem cell differentiation into neural stem cells, isoform 1 expression increases, whereas ΔN-RNF220 expression decreases. In murine myoblasts, ΔN-RNF220 is the sole expressed isoform and is required for MyoD and myogenin expression, as well as for muscle differentiation. Our findings highlight ΔN-RNF220 as the ubiquitously and highly expressed variant, likely playing a fundamental role across tissues while exhibiting functional differences from isoform 1. These results emphasize the critical importance of ΔN-RNF220 in future studies investigating the biological functions of RNF220.</div></div>","PeriodicalId":18795,"journal":{"name":"Molecules and Cells","volume":"48 9","pages":"Article 100250"},"PeriodicalIF":3.7,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144560537","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Brief guide to Caenorhabditis elegans imaging and quantification 秀丽隐杆线虫成像与定量简介。

IF 3.7 3区生物学

Molecules and Cells Pub Date : 2025-06-29 DOI: 10.1016/j.mocell.2025.100249

Hyemin Min, Gyutae Park, Seung-Jae V. Lee

引用次数: 0

Advancing precision diagnosis in autism: Insights from large-scale genomic studies 推进自闭症的精确诊断：来自大规模基因组研究的见解。

IF 3.7 3区生物学

Molecules and Cells Pub Date : 2025-06-26 DOI: 10.1016/j.mocell.2025.100248

Soo-Whee Kim , Joon-Yong An

{"title":"Advancing precision diagnosis in autism: Insights from large-scale genomic studies","authors":"Soo-Whee Kim , Joon-Yong An","doi":"10.1016/j.mocell.2025.100248","DOIUrl":"10.1016/j.mocell.2025.100248","url":null,"abstract":"<div><div>Autism spectrum disorder (ASD) is a neurodevelopmental condition with a complex genetic basis. Large-scale whole-exome sequencing and whole-genome sequencing studies, with increasing sample sizes and improved ancestral diversity, have significantly advanced the discovery of ASD-associated genes. In addition to identifying coding variants, whole-genome sequencing has facilitated the detection of risk noncoding variants in regulatory elements such as enhancers, promoters, and untranslated regions, prompting experimental validation of their functional impact on neurodevelopment. A deeper understanding of ASD genetic liability has revealed the interplay between rare and common variants. Moreover, genetic liability varies by sex and phenotype profile, underscoring the complexity of ASD’s genetic architecture. While the clinical application of these genomic insights remains in early stages, progress has been made in gene-based therapeutic development, the interpretation of noncoding risk variants, and the use of polygenic score for risk stratification. In this review, we summarize key findings from large-scale genomic studies, explore the role of coding and noncoding variants in ASD, and discuss emerging opportunities for translating these discoveries into clinical practice.</div></div>","PeriodicalId":18795,"journal":{"name":"Molecules and Cells","volume":"48 8","pages":"Article 100248"},"PeriodicalIF":3.7,"publicationDate":"2025-06-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144528883","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Optogenetics to biomolecular phase separation in neurodegenerative diseases 神经退行性疾病的光遗传学与生物分子相分离。

IF 3.7 3区生物学

Molecules and Cells Pub Date : 2025-06-22 DOI: 10.1016/j.mocell.2025.100247

Kyung Hwan Park, Kyung Won Kim

{"title":"Optogenetics to biomolecular phase separation in neurodegenerative diseases","authors":"Kyung Hwan Park, Kyung Won Kim","doi":"10.1016/j.mocell.2025.100247","DOIUrl":"10.1016/j.mocell.2025.100247","url":null,"abstract":"<div><div>Neurodegenerative diseases involve toxic protein aggregation. Recent evidence suggests that biomolecular phase separation, a process in which proteins and nucleic acids form dynamic, liquid-like condensates, plays a key role in this aggregation. Optogenetics, originally developed to control neuronal activity with light, has emerged as a powerful tool to investigate phase separation in living systems. This is achieved by fusing disease-associated proteins to light-sensitive oligomerization domains, enabling researchers to induce or reverse condensate formation with precise spatial and temporal control. This review highlights how optogenetic systems such as OptoDroplet are being used to dissect the mechanisms of neurodegenerative disease. We examine how these tools have been applied in models of neurodegenerative diseases, such as amyotrophic lateral sclerosis, Alzheimer’s, Parkinson’s, and Huntington’s disease. These studies implicate small oligomeric aggregates as key drivers of toxicity and highlight new opportunities for therapeutic screening. Finally, we discuss advances in light-controlled dissolution of condensates and future directions for applying optogenetics to combat neurodegeneration. By enabling precise, dynamic control of protein phase behavior in living systems, optogenetic approaches provide a powerful framework for elucidating disease mechanisms and informing the development of targeted therapies.</div></div>","PeriodicalId":18795,"journal":{"name":"Molecules and Cells","volume":"48 8","pages":"Article 100247"},"PeriodicalIF":3.7,"publicationDate":"2025-06-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144485135","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0