Molecules and Cells最新文献_第3页

The role of transcription factors in prostate cancer progression 转录因子在前列腺癌进展中的作用。

IF 3.7 3区生物学

Molecules and Cells Pub Date : 2025-02-10 DOI: 10.1016/j.mocell.2025.100193

Jongeun Lee , Yoontae Lee

引用次数: 0

Biallelic variants of SEMA3F are associated with nonsyndromic hearing loss SEMA3F的双等位基因变异与非综合征性听力损失有关。

IF 3.7 3区生物学

Molecules and Cells Pub Date : 2025-02-03 DOI: 10.1016/j.mocell.2025.100190

Sun Young Joo , Hyehyun Min , Jung Ah Kim , Se Jin Kim , Seung Hyun Jang , Ho Lee , Kyu Min Kim , Je Kyung Seong , Jae Young Choi , Jinsei Jung , Jinwoong Bok , Heon Yung Gee

{"title":"Biallelic variants of SEMA3F are associated with nonsyndromic hearing loss","authors":"Sun Young Joo , Hyehyun Min , Jung Ah Kim , Se Jin Kim , Seung Hyun Jang , Ho Lee , Kyu Min Kim , Je Kyung Seong , Jae Young Choi , Jinsei Jung , Jinwoong Bok , Heon Yung Gee","doi":"10.1016/j.mocell.2025.100190","DOIUrl":"10.1016/j.mocell.2025.100190","url":null,"abstract":"<div><div>It is crucial to manage hearing loss and its associated public health impacts. In this study, we aimed to understand the role of Sema3f in the development and maintenance of the auditory system. Inner ear-specific <em>Sema3f</em> knockout mice exhibited hearing loss at 8 weeks with an elevated threshold for auditory brainstem response and an absent threshold for distortion product optoacoustic emission tests. Additionally, an increased number of outer hair cells and abnormal patterns of spiral ganglion neuron projections in the outer hair cell regions were observed. Through the analyses of sequencing data from 558 families with hearing loss, we identified biallelic variants of <em>SEMA3F</em>, which encodes semaphorin-3F, in one of the families. In the family, the proband showed profound progressive nonsyndromic hearing loss with congenital onset. In vitro analysis revealed that the identified missense variants decreased the furin-mediated processing of SEMA3F and abolished the cellular abilities of SEMA3F, which collapsed the filamentous actin cytoskeleton in human umbilical vein-derived endothelial cells. Our data suggest that <em>SEMA3F</em> is essential for normal hearing and is associated with nonsyndromic hearing loss in humans.</div></div>","PeriodicalId":18795,"journal":{"name":"Molecules and Cells","volume":"48 3","pages":"Article 100190"},"PeriodicalIF":3.7,"publicationDate":"2025-02-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143256124","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A brief guide for gene delivery to the brain using adeno-associated viral vectors 使用腺相关病毒载体向大脑输送基因的简要指南。

IF 3.7 3区生物学

Molecules and Cells Pub Date : 2025-02-02 DOI: 10.1016/j.mocell.2025.100189

Seungwan Han , Eun Mo Yang , Eun-Mi Hur

引用次数: 0

eIF2α phosphorylation-ATF4 axis-mediated transcriptional reprogramming mitigates mitochondrial impairment during ER stress eIF2α磷酸化- atf4轴介导的转录重编程减轻内质网应激时线粒体损伤。

IF 3.7 3区生物学

Molecules and Cells Pub Date : 2025-02-01 DOI: 10.1016/j.mocell.2024.100176

Hien Thi Le , Jiyoung Yu , Hee Sung Ahn , Mi-Jeong Kim , In Gyeong Chae , Hyun-Nam Cho , Juhee Kim , Hye-Kyung Park , Hyuk Nam Kwon , Han-Jung Chae , Byoung Heon Kang , Jeong Kon Seo , Kyunggon Kim , Sung Hoon Back

{"title":"eIF2α phosphorylation-ATF4 axis-mediated transcriptional reprogramming mitigates mitochondrial impairment during ER stress","authors":"Hien Thi Le , Jiyoung Yu , Hee Sung Ahn , Mi-Jeong Kim , In Gyeong Chae , Hyun-Nam Cho , Juhee Kim , Hye-Kyung Park , Hyuk Nam Kwon , Han-Jung Chae , Byoung Heon Kang , Jeong Kon Seo , Kyunggon Kim , Sung Hoon Back","doi":"10.1016/j.mocell.2024.100176","DOIUrl":"10.1016/j.mocell.2024.100176","url":null,"abstract":"<div><div>Eukaryotic translation initiation factor 2α (eIF2α) phosphorylation, which regulates all 3 unfolded protein response pathways, helps maintain cellular homeostasis and overcome endoplasmic reticulum (ER) stress through transcriptional and translational reprogramming. However, transcriptional regulation of mitochondrial homeostasis by eIF2α phosphorylation during ER stress is not fully understood. Here, we report that the eIF2α phosphorylation-activating transcription factor 4 (ATF4) axis is required for the expression of multiple transcription factors, including nuclear factor erythroid 2-related factor 2 and its target genes responsible for mitochondrial homeostasis during ER stress. eIF2α phosphorylation-deficient (<em>A/A</em>) cells displayed dysregulated mitochondrial dynamics and mitochondrial DNA replication, decreased expression of oxidative phosphorylation complex proteins, and impaired mitochondrial functions during ER stress. ATF4 overexpression suppressed impairment of mitochondrial homeostasis in <em>A/A</em> cells during ER stress by promoting the expression of downstream transcription factors and their target genes. Our findings underscore the importance of the eIF2α phosphorylation-ATF4 axis for maintaining mitochondrial homeostasis through transcriptional reprogramming during ER stress.</div></div>","PeriodicalId":18795,"journal":{"name":"Molecules and Cells","volume":"48 2","pages":"Article 100176"},"PeriodicalIF":3.7,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11786836/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142932314","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Autophagic signatures in peripheral blood mononuclear cells from Parkinson's disease patients 帕金森病患者外周血单个核细胞的自噬特征。

IF 3.7 3区生物学

Molecules and Cells Pub Date : 2025-02-01 DOI: 10.1016/j.mocell.2024.100173

Myung Shin Lee , Jae Whan Kim , Don Gueu Park , Hansol Heo , Juyeong Kim , Jung Han Yoon , Jaerak Chang

{"title":"Autophagic signatures in peripheral blood mononuclear cells from Parkinson's disease patients","authors":"Myung Shin Lee , Jae Whan Kim , Don Gueu Park , Hansol Heo , Juyeong Kim , Jung Han Yoon , Jaerak Chang","doi":"10.1016/j.mocell.2024.100173","DOIUrl":"10.1016/j.mocell.2024.100173","url":null,"abstract":"<div><div>Parkinson's disease (PD) is a progressive neurodegenerative disorder characterized by motor impairments and the accumulation of misfolded α-synuclein. Dysregulation of the autophagy-lysosomal pathway (ALP), responsible for degrading misfolded proteins, has been implicated in PD pathogenesis. However, current diagnostic approaches rely heavily on motor symptoms, which occur due to substantial neurodegeneration, limiting early detection and intervention. This study investigated the potential of ALP-associated proteins in peripheral blood mononuclear cells (PBMCs) as diagnostic biomarkers for early-stage PD. Quantitative analysis revealed a significant reduction in optineurin levels in PBMCs from PD patients, and the expression levels of various ALP-associated proteins were tightly correlated, suggesting a coordinated dysregulation of the pathway. Correlation analyses revealed associations between ALP-associated features and clinical characteristics, such as age of onset and motor impairment. Furthermore, the study identified multiple positive correlations among ALP-associated proteins and functional readouts, highlighting the interconnectivity within the pathway. Notably, a PBMC biomarker model incorporating lysosomal-associated membrane protein 1 and optineurin exhibited high diagnostic accuracy (86%) in distinguishing PD patients from controls. These findings highlight the potential of ALP-associated protein signatures in PBMCs as novel diagnostic biomarkers for early detection and intervention in PD, offering insights into the systemic manifestations of the disease.</div></div>","PeriodicalId":18795,"journal":{"name":"Molecules and Cells","volume":"48 2","pages":"Article 100173"},"PeriodicalIF":3.7,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11786884/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142895808","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Tumor cells ectopically expressing the membrane-bound form of IL-7 develop an antitumor immune response efficiently in a colon carcinoma model 在结肠癌模型中，异位表达IL-7膜结合形式的肿瘤细胞有效地产生抗肿瘤免疫反应。

IF 3.7 3区生物学

Molecules and Cells Pub Date : 2025-02-01 DOI: 10.1016/j.mocell.2024.100175

Hee-Su Shin , Hyejin Kim , Soon-Gyu Kwon , Hayyoung Lee , Jie-Oh Lee , Young Sang Kim

{"title":"Tumor cells ectopically expressing the membrane-bound form of IL-7 develop an antitumor immune response efficiently in a colon carcinoma model","authors":"Hee-Su Shin , Hyejin Kim , Soon-Gyu Kwon , Hayyoung Lee , Jie-Oh Lee , Young Sang Kim","doi":"10.1016/j.mocell.2024.100175","DOIUrl":"10.1016/j.mocell.2024.100175","url":null,"abstract":"<div><div>Various approaches employing cytokines and cytokine gene–modified tumor cells have been explored to induce antitumor responses, yet their widespread application has been limited due to efficacy concerns and adverse effects. In this study, interleukin-7 was engineered for expression both as a natural secretory form (sIL-7) and as a membrane-bound form fused with the B7.1 type I transmembrane protein (mbIL-7/B7) on CT26 colon cancer cells. Analysis of the resulting cell clones demonstrated that ectopically expressed sIL-7 and mbIL-7/B7 both retained similar capacities to induce the expansion and activation of CD8<sup>+</sup> T cells and to enhance antitumor responses in vitro. While the sIL-7 or mbIL-7/B7 clones showed similar growth in culture, the mbIL-7/B7 clone exhibited lower tumorigenicity in mice compared with the sIL-7 clone or wild-type CT26 cells. Specifically, the mbIL-7/B7 clone failed to form tumors in approximately 60% of the mice injected with it. Moreover, 80% of mice that rejected the mbIL-7/B7 clone developed long-term systemic immunity against CT26 cells. Analysis of immune cells within the tumor masses revealed significant increases in CD4<sup>+</sup> T cells, CD8<sup>+</sup> T cells, and dendritic cells in tumors formed by the mbIL-7/B7 clone compared to those formed by the sIL-7 clone. These findings suggest that the membrane-bound form of IL-7 with B7.1 is more effective than the secretory form in establishing antitumor immunity within the tumor microenvironment. Our strategy of expressing the mbIL-7/B7 chimera holds promise as a novel approach for tumor therapy, particularly in cases requiring IL-7 supplementation.</div></div>","PeriodicalId":18795,"journal":{"name":"Molecules and Cells","volume":"48 2","pages":"Article 100175"},"PeriodicalIF":3.7,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142914973","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Essential resources and best practices for laboratory mouse research 实验室小鼠研究的基本资源和最佳实践。

IF 3.7 3区生物学

Molecules and Cells Pub Date : 2025-02-01 DOI: 10.1016/j.mocell.2025.100178

Rosa Haque , Aysenur Deniz Song , Jongsun Lee , Seung-Jae V. Lee , Jae Myoung Suh

引用次数: 0

Cancer prognosis using base excision repair genes 碱基切除修复基因对癌症预后的影响。

IF 3.7 3区生物学

Molecules and Cells Pub Date : 2025-02-01 DOI: 10.1016/j.mocell.2025.100186

Jeongeun Kim , Su-Jin Kang , Nayoon Jo , Seung-Jin Kim , Sunbok Jang

{"title":"Cancer prognosis using base excision repair genes","authors":"Jeongeun Kim , Su-Jin Kang , Nayoon Jo , Seung-Jin Kim , Sunbok Jang","doi":"10.1016/j.mocell.2025.100186","DOIUrl":"10.1016/j.mocell.2025.100186","url":null,"abstract":"<div><div>The base excision repair (BER) pathway is a critical mechanism in genomic stability. This review investigates the role of the BER pathway in advanced cancer therapies considering the pivotal role of genetic factors in cancer patient responses and prognosis. BER factors significantly influence genetic instability and cancer prognosis, as well as the effectiveness of chemotherapy and radiation therapy. In various cancers such as breast, colon, lung, and bladder, BER factors have shown potential as critical biological markers for predicting cancer outcomes. This study focuses on the polymorphisms and expression levels of key BER genes, including OGG1, XRCC1, APE1, and Polβ. Our findings demonstrate that the expression levels of BER genes and proteins are closely associated with the risk, progression, treatment response, and prognosis of various cancers. These insights could improve cancer treatments and aid in the development of drugs targeting BER proteins. Ongoing research in this field requires extensive statistical analyses and large-scale prospective studies to effectively utilize BER protein levels. Ultimately, these results suggest that the BER pathway represents a potential target for cancer diagnosis, prognostic prediction, and the development of personalized therapeutic strategies. This paves the way for effective cancer treatment in the future.</div></div>","PeriodicalId":18795,"journal":{"name":"Molecules and Cells","volume":"48 2","pages":"Article 100186"},"PeriodicalIF":3.7,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143008576","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Cover and caption 封面及标题

IF 3.7 3区生物学

Molecules and Cells Pub Date : 2025-02-01 DOI: 10.1016/S1016-8478(25)00018-4

引用次数: 0

Editorial Board Members/Copyright 编辑委员会成员/版权

IF 3.7 3区生物学

Molecules and Cells Pub Date : 2025-02-01 DOI: 10.1016/S1016-8478(25)00020-2

引用次数: 0