Brief guide to Caenorhabditis elegans imaging and quantification

IF 6.5 3区生物学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY

Molecules and Cells Pub Date : 2025-06-29 DOI:10.1016/j.mocell.2025.100249

Hyemin Min, Gyutae Park, Seung-Jae V. Lee

引用次数: 0

Abstract

Caenorhabditis elegans is a powerful model organism for fluorescence imaging to study gene regulation and cellular biomarkers. This concise MiniResource introduces essential imaging techniques for live and fixed C. elegans, sample preparations, microscopy, and common applications. We also outline basic image analysis workflows using open-source software. This guideline serves as a practical resource for researchers who seek implementing fluorescence imaging with C. elegans.

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秀丽隐杆线虫成像与定量简介。

秀丽隐杆线虫是荧光成像研究基因调控和细胞生物标志物的有力模式生物。这个简洁的MiniResource介绍了活体和固定秀丽隐杆线虫，样品制备，显微镜和常见应用的基本成像技术。我们还概述了使用开源软件的基本图像分析工作流程。本指南作为一个实用的资源，为研究人员寻求实现荧光成像与秀丽隐杆线虫。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Molecules and Cells 生物-生化与分子生物学

CiteScore

6.60

自引率

10.50%

发文量

审稿时长

2.3 months

期刊介绍： Molecules and Cells is an international on-line open-access journal devoted to the advancement and dissemination of fundamental knowledge in molecular and cellular biology. It was launched in 1990 and ISO abbreviation is "Mol. Cells". Reports on a broad range of topics of general interest to molecular and cell biologists are published. It is published on the last day of each month by the Korean Society for Molecular and Cellular Biology.